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Parallel optical read-out of micromechanical pillars applied to prostate specific membrane antigen detection
- Source :
- Biosensors & bioelectronics 72 (2015): 393–399. doi:10.1016/j.bios.2015.05.026, info:cnr-pdr/source/autori:Tardivo, Martina; Toffoli, Valeria; Fracasso, Giulio; Borin, Daniele; Dal Zilio, Simone; Colusso, Andrea; Carrato, Sergio; Scoles, Giacinto; Meneghetti, Moreno; Colombatti, Marco; Lazzarino, Marco/titolo:Parallel optical read-out of micromechanical pillars applied to prostate specific membrane antigen detection/doi:10.1016%2Fj.bios.2015.05.026/rivista:Biosensors & bioelectronics/anno:2015/pagina_da:393/pagina_a:399/intervallo_pagine:393–399/volume:72
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Micro and nanomechanical resonators represent a promising platform for proteins label-free detection because of their extreme sensitivity, fast response and low cost. Micro-pillars are columnar resonators that can be easily arranged in dense arrays of several thousand sensors in a squared mm. To exploit such a large density, however, a method for tracking independently micropillars resonance frequency is required. Here we present a detection method based on CCD imaging and software image analysis, which can measure the resonance frequency of tens of pillars in parallel. Acquiring simultaneously the frequency shift of up to 40 sensors and applying a proper statistical analysis, we were able to overcome the variability of the single measures improving the device sensitivity at low analyte concentration range. As a proof of concept, this method has been tested for the detection of a tumor marker, the Prostate Specific Membrane Antigen (PSMA). Pillars have been functionalized with an antibody against PSMA. The tumor marker (PSMA) has been detected in a range of concentrations between 300 pM and 100 nM, in buffer and in diluted bovine serum. The sensitivity of our method was limited only by the affinity constant of the antigen–antibody recognition. Moreover, this detection technique demonstrated to be effective in the 1–6 nM range, which is the window of PSMA concentration of clinical interest.
- Subjects :
- Male
Analyte
Materials science
Biomedical Engineering
Biophysics
Frequency shift
Nanotechnology
Biosensing Techniques
Tracking (particle physics)
Buffer (optical fiber)
parallel optical read-out detection
Resonator
Limit of Detection
Biomarkers, Tumor
PSMA
Electrochemistry
Glutamate carboxypeptidase II
Humans
Micromechanical sensors
Parallel optical read-out detection
Prostate specific membrane antigen
Biotechnology
Micromechanical sensors Parallel optical read-out detection Prostate Specific Membrane Antigen
Prostate
Prostatic Neoplasms
Equipment Design
General Medicine
Micro-Electrical-Mechanical Systems
Prostate-Specific Antigen
biosensors
prostate cancer
Prostate Specific Membrane Antigen
Antibodies, Immobilized
Biosensor
Sensitivity (electronics)
Biomedical engineering
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....52887c5e3ee2c1d3768d6674f46e091f