2,389 results on '"Bizzotto A"'
Search Results
2. Increasing situational awareness through nowcasting of the reproduction number
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Bizzotto, Andrea, Guzzetta, Giorgio, Marziano, Valentina, del Manso, Martina, Urdiales, Alberto Mateo, Petrone, Daniele, Cannone, Andrea, Sacco, Chiara, Poletti, Piero, Manica, Mattia, Zardini, Agnese, Trentini, Filippo, Fabiani, Massimo, Bella, Antonino, Riccardo, Flavia, Pezzotti, Patrizio, Ajelli, Marco, and Merler, Stefano
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Quantitative Biology - Populations and Evolution - Abstract
The time varying reproduction number R is a critical variable for situational awareness during infectious disease outbreaks, but delays between infection and reporting hinder its accurate estimation in real time. We propose a nowcasting method for improving the timeliness and accuracy of R estimates, based on comparisons of successive versions of surveillance databases. The method was validated against COVID-19 surveillance data collected in Italy over an 18-month period. Compared to traditional methods, the nowcasted reproduction number reduced the estimation delay from 13 to 8 days, while maintaining a better accuracy. Moreover, it allowed anticipating the detection of periods of epidemic growth by between 6 and 23 days. The method offers a simple and generally applicable tool to improve situational awareness during an epidemic outbreak, allowing for informed public health response planning.
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- 2023
3. Dibujar in situ: estudio de una experiencia corporal para investigar el espacio vivido
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Lucas Javier Bizzotto
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cognición encarnada ,encuentros ,fenomenología ,percepción ,Architecture ,NA1-9428 - Abstract
Este trabajo analiza el acto de dibujar como una experiencia corporal situada de percepción, comprensión y comunicación del espacio vivido. Es sabido que las experiencias son la base para renovar el examen, la interpretación y la teoría arquitectónica. Numerosos estudios han señalado que dibujar juega un papel central en la naturaleza de la cognición y conceptualización arquitectónica. Así, los dibujos son herramientas no solo para proyectar, sino también para generar nuevos conocimientos. Se sostiene aquí que dibujar, como un acto de consciencia corporal, encarna lo percibido por el dibujante y se presenta como relato de experiencia. Las conclusiones sugieren que dibujar in situ favorece una serie de encuentros y, como investigación del espacio vivido, es una forma peculiar de cognición corporal que se externaliza para comunicar una experiencia singular.
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- 2024
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4. MICROPHERRET: MICRObial PHEnotypic tRait ClassifieR using Machine lEarning Techniques
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Edoardo Bizzotto, Sofia Fraulini, Guido Zampieri, Esteban Orellana, Laura Treu, and Stefano Campanaro
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Functional classification ,Machine learning ,Microbial genome ,Metagenome ,Methanogenesis ,Environmental sciences ,GE1-350 ,Microbiology ,QR1-502 - Abstract
Abstract Background In recent years, there has been a rapid increase in the number of microbial genomes reconstructed through shotgun sequencing, and obtained by newly developed approaches including metagenomic binning and single-cell sequencing. However, our ability to functionally characterize these genomes by experimental assays is orders of magnitude less efficient. Consequently, there is a pressing need for the development of swift and automated strategies for the functional classification of microbial genomes. Results The present work leverages a suite of supervised machine learning algorithms to establish a range of 86 metabolic and other ecological functions, such as methanotrophy and plastic degradation, starting from widely obtainable microbial genome annotations. Tests performed on independent datasets demonstrated robust performance across complete, fragmented, and incomplete genomes above a 70% completeness level for most of the considered functions. Application of the algorithms to the Biogas Microbiome database yielded predictions broadly consistent with current biological knowledge and correctly detecting functionally-related nuances of archaeal genomes. Finally, a case study focused on acetoclastic methanogenesis demonstrated how the developed machine learning models can be refined or expanded with models describing novel functions of interest. Conclusions The resulting tool, MICROPHERRET, incorporates a total of 86 models, one for each tested functional class, and can be applied to high-quality microbial genomes as well as to low-quality genomes derived from metagenomics and single-cell sequencing. MICROPHERRET can thus aid in understanding the functional role of newly generated genomes within their micro-ecological context.
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- 2024
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5. Classification of bioactive peptides: A systematic benchmark of models and encodings
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Edoardo Bizzotto, Guido Zampieri, Laura Treu, Pasquale Filannino, Raffaella Di Cagno, and Stefano Campanaro
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Bioactive peptide ,Functional classification ,Machine learning ,Sequence encoding ,Systematic evaluation ,Biotechnology ,TP248.13-248.65 - Abstract
Bioactive peptides are short amino acid chains possessing biological activity and exerting physiological effects relevant to human health. Despite their therapeutic value, their identification remains a major problem, as it mainly relies on time-consuming in vitro tests. While bioinformatic tools for the identification of bioactive peptides are available, they are focused on specific functional classes and have not been systematically tested on realistic settings. To tackle this problem, bioactive peptide sequences and functions were here gathered from a variety of databases to generate a unified collection of bioactive peptides from microbial fermentation. This collection was organized into nine functional classes including some previously studied and some unexplored such as immunomodulatory, opioid and cardiovascular peptides. Upon assessing their sequence properties, four alternative encoding methods were tested in combination with a multitude of machine learning algorithms, from basic classifiers like logistic regression to advanced algorithms like BERT. Tests on a total of 171 models showed that, while some functions are intrinsically easier to detect, no single combination of classifiers and encoders worked universally well for all classes. For this reason, we unified all the best individual models for each class and generated CICERON (Classification of bIoaCtive pEptides fRom micrObial fermeNtation), a classification tool for the functional classification of peptides. State-of-the-art classifiers were found to underperform on our realistic benchmark dataset compared to the models included in CICERON. Altogether, our work provides a tool for real-world peptide classification and can serve as a benchmark for future model development.
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- 2024
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6. MICROPHERRET: MICRObial PHEnotypic tRait ClassifieR using Machine lEarning Techniques
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Bizzotto, Edoardo, Fraulini, Sofia, Zampieri, Guido, Orellana, Esteban, Treu, Laura, and Campanaro, Stefano
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- 2024
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7. Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12
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Santin, Anna, Collura, Flavio, Singh, Garima, Morlino, Maria Silvia, Bizzotto, Edoardo, Bellan, Alessandra, Gupte, Ameya Pankaj, Favaro, Lorenzo, Campanaro, Stefano, Treu, Laura, and Morosinotto, Tomas
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- 2024
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8. GBA1 inactivation in oligodendrocytes affects myelination and induces neurodegenerative hallmarks and lipid dyshomeostasis in mice
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Gregorio, Ilaria, Russo, Loris, Torretta, Enrica, Barbacini, Pietro, Contarini, Gabriella, Pacinelli, Giada, Bizzotto, Dario, Moriggi, Manuela, Braghetta, Paola, Papaleo, Francesco, Gelfi, Cecilia, Moro, Enrico, and Cescon, Matilde
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- 2024
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9. Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12
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Anna Santin, Flavio Collura, Garima Singh, Maria Silvia Morlino, Edoardo Bizzotto, Alessandra Bellan, Ameya Pankaj Gupte, Lorenzo Favaro, Stefano Campanaro, Laura Treu, and Tomas Morosinotto
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Cyanobacteria ,Poly-3-hydroxybutyrate ,Light ,Starvation ,Transcriptomics ,Biotechnology ,TP248.13-248.65 ,Fuel ,TP315-360 - Abstract
Abstract Background Microbial biopolymers such as poly-3-hydroxybutyrate (PHB) are emerging as promising alternatives for sustainable production of biodegradable bioplastics. Their promise is heightened by the potential utilisation of photosynthetic organisms, thus exploiting sunlight and carbon dioxide as source of energy and carbon, respectively. The cyanobacterium Synechocystis sp. B12 is an attractive candidate for its superior ability to accumulate high amounts of PHB as well as for its high-light tolerance, which makes it extremely suitable for large-scale cultivation. Beyond its practical applications, B12 serves as an intriguing model for unravelling the molecular mechanisms behind PHB accumulation. Results Through a multifaceted approach, integrating physiological, genomic and transcriptomic analyses, this work identified genes involved in the upregulation of chlorophyll biosynthesis and phycobilisome degradation as the possible candidates providing Synechocystis sp. B12 an advantage in growth under high-light conditions. Gene expression differences in pentose phosphate pathway and acetyl-CoA metabolism were instead recognised as mainly responsible for the increased Synechocystis sp. B12 PHB production during nitrogen starvation. In both response to strong illumination and PHB accumulation, Synechocystis sp. B12 showed a metabolic modulation similar but more pronounced than the reference strain, yielding in better performances. Conclusions Our findings shed light on the molecular mechanisms of PHB biosynthesis, providing valuable insights for optimising the use of Synechocystis in economically viable and sustainable PHB production. In addition, this work supplies crucial knowledge about the metabolic processes involved in production and accumulation of these molecules, which can be seminal for the application to other microorganisms as well.
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- 2024
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10. Predicting prostate cancer progression with a Multi-lncRNA expression-based risk score and nomogram integrating ISUP grading
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Sabrina Ledesma-Bazan, Florencia Cascardo, Juan Bizzotto, Santiago Olszevicki, Elba Vazquez, Geraldine Gueron, and Javier Cotignola
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Genetics ,QH426-470 - Abstract
Prostate cancer is a highly heterogeneous disease; therefore, estimating patient prognosis accurately is challenging due to the lack of biomarkers with sufficient specificity and sensitivity. One of the current challenges lies in integrating genomic and transcriptomic data with clinico-pathological features and in incorporating their application in everyday clinical practice. Therefore, we aimed to model a risk score and nomogram containing long non-coding RNA (lncRNA) expression and clinico-pathological data to better predict the probability of prostate cancer progression. We performed bioinformatics analyses to identify lncRNAs differentially expressed across various prostate cancer stages and associated with progression-free survival. This information was further integrated into a prognostic risk score and nomogram containing transcriptomic and clinico-pathological features to estimate the risk of disease progression. We used RNA-seq data from 5 datasets from public repositories (total n = 178) comprising different stages of prostate cancer: pre-treatment primary prostate adenocarcinomas, post-treatment tumors and metastatic castration resistant prostate cancer. We found 30 lncRNAs with consistent differential expression in all comparisons made using two R-based packages. Multivariate progression-free survival analysis including the ISUP group as covariate, revealed that 7/30 lncRNAs were significantly associated with time-to-progression. Next, we combined the expression of these 7 lncRNAs into a multi-lncRNA score and dichotomized the patients into low- or high-score. Patients with a high-score showed a 4-fold risk of disease progression (HR = 4.30, 95 %CI = 2.66–6.97, p = 3.1e-9). Furthermore, we modelled a combined risk-score containing information on the multi-lncRNA score and ISUP group. We found that patients with a high-risk score had nearly 8-fold risk of progression (HR = 7.65, 95 %CI = 4.05–14.44, p = 3.4e-10). Finally, we created and validated a nomogram to help uro-oncologists to better predict patient's risk of progression at 3- and 5-years post-diagnosis. In conclusion, the integration of lncRNA expression data and clinico-pathological features of prostate tumors into predictive models might aid in tailored disease risk assessment and treatment for patients with prostate cancer.
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- 2024
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11. Variable positive end-expiratory pressure in an experimental model of acute respiratory distress syndrome: an advanced ventilation modality
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Roberta Südy, John Diaper, Davide Bizzotto, Rafaelle Dellàca, Ferenc Petak, Walid Habre, and Andre Dos Santos Rocha
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gas exchange ,lung function ,lung oxygenation index ,variable ventilation ,ventilator-induced lung injury ,Anesthesiology ,RD78.3-87.3 - Abstract
Background: Introducing variability in tidal volume, ventilatory frequency, or both is beneficial during mechanical ventilation in acute respiratory distress syndrome (ARDS). We investigated whether applying cycle-by-cycle variability in the positive end-expiratory pressure (PEEP) exerts beneficial effect on lung function in a model of ARDS. Methods: Rabbits with lung injury were randomly allocated to receive mechanical ventilation for 6 h by applying a pressure-controlled mode with constant PEEP of 7 cm H2O (PC group: n=6) or variable PEEP (VEEP) with a coefficient of variation of 21.4%, range 4–10 cm H2O (PC-VEEP group; n=6). Lung oxygenation index (Pao2/FiO2) after 6 h of ventilation (H6) was the primary outcome and respiratory mechanics, lung volume, intrapulmonary shunt, and lung inflammatory markers were secondary outcomes. Results: After lung injury, both groups presented moderate-to-severe ARDS (Pao2/FiO2
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- 2024
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12. Increasing situational awareness through nowcasting of the reproduction number
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Andrea Bizzotto, Giorgio Guzzetta, Valentina Marziano, Martina Del Manso, Alberto Mateo Urdiales, Daniele Petrone, Andrea Cannone, Chiara Sacco, Piero Poletti, Mattia Manica, Agnese Zardini, Filippo Trentini, Massimo Fabiani, Antonino Bella, Flavia Riccardo, Patrizio Pezzotti, Marco Ajelli, and Stefano Merler
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reproduction number ,situational awareness ,epidemic surveillance ,nowcasting ,outbreaks ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundThe time-varying reproduction number R is a critical variable for situational awareness during infectious disease outbreaks; however, delays between infection and reporting of cases hinder its accurate estimation in real-time. A number of nowcasting methods, leveraging available information on data consolidation delays, have been proposed to mitigate this problem.MethodsIn this work, we retrospectively validate the use of a nowcasting algorithm during 18 months of the COVID-19 pandemic in Italy by quantitatively assessing its performance against standard methods for the estimation of R.ResultsNowcasting significantly reduced the median lag in the estimation of R from 13 to 8 days, while concurrently enhancing accuracy. Furthermore, it allowed the detection of periods of epidemic growth with a lead of between 6 and 23 days.ConclusionsNowcasting augments epidemic awareness, empowering better informed public health responses.
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- 2024
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13. Sorting and Grading
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Bizzotto, Jacopo and Vigier, Adrien
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Economics - Theoretical Economics - Abstract
We propose a framework to assess how to optimally sort and grade students of heterogenous ability. Potential employers face uncertainty regarding an individual's productive value. Knowing which school an individual went to is useful for two reasons: firstly, average student ability may differ across schools; secondly, different schools may use different grading rules and thus provide varying incentives to exert effort. An optimal school system exhibits coarse stratification with respect to ability, and more lenient grading at the top-tier schools than at the bottom-tier schools. Our paper contributes to the ongoing policy debate on tracking in secondary schools., Comment: 27 pages, 1 figure
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- 2022
14. GBA1 inactivation in oligodendrocytes affects myelination and induces neurodegenerative hallmarks and lipid dyshomeostasis in mice
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Ilaria Gregorio, Loris Russo, Enrica Torretta, Pietro Barbacini, Gabriella Contarini, Giada Pacinelli, Dario Bizzotto, Manuela Moriggi, Paola Braghetta, Francesco Papaleo, Cecilia Gelfi, Enrico Moro, and Matilde Cescon
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Parkinson’s disease ,Gaucher disease ,Oligodendrocyte ,White matter ,β-glucocerebrosidase ,Myelination ,Neurology. Diseases of the nervous system ,RC346-429 ,Geriatrics ,RC952-954.6 - Abstract
Abstract Background Mutations in the β-glucocerebrosidase (GBA1) gene do cause the lysosomal storage Gaucher disease (GD) and are among the most frequent genetic risk factors for Parkinson’s disease (PD). So far, studies on both neuronopathic GD and PD primarily focused on neuronal manifestations, besides the evaluation of microglial and astrocyte implication. White matter alterations were described in the central nervous system of paediatric type 1 GD patients and were suggested to sustain or even play a role in the PD process, although the contribution of oligodendrocytes has been so far scarcely investigated. Methods We exploited a system to study the induction of central myelination in vitro, consisting of Oli-neu cells treated with dibutyryl-cAMP, in order to evaluate the expression levels and function of β-glucocerebrosidase during oligodendrocyte differentiation. Conduritol-B-epoxide, a β-glucocerebrosidase irreversible inhibitor was used to dissect the impact of β-glucocerebrosidase inactivation in the process of myelination, lysosomal degradation and α-synuclein accumulation in vitro. Moreover, to study the role of β-glucocerebrosidase in the white matter in vivo, we developed a novel mouse transgenic line in which β-glucocerebrosidase function is abolished in myelinating glia, by crossing the Cnp1-cre mouse line with a line bearing loxP sequences flanking Gba1 exons 9–11, encoding for β-glucocerebrosidase catalytic domain. Immunofluorescence, western blot and lipidomic analyses were performed in brain samples from wild-type and knockout animals in order to assess the impact of genetic inactivation of β-glucocerebrosidase on myelination and on the onset of early neurodegenerative hallmarks, together with differentiation analysis in primary oligodendrocyte cultures. Results Here we show that β-glucocerebrosidase inactivation in oligodendrocytes induces lysosomal dysfunction and inhibits myelination in vitro. Moreover, oligodendrocyte-specific β-glucocerebrosidase loss-of-function was sufficient to induce in vivo demyelination and early neurodegenerative hallmarks, including axonal degeneration, α-synuclein accumulation and astrogliosis, together with brain lipid dyshomeostasis and functional impairment. Conclusions Our study sheds light on the contribution of oligodendrocytes in GBA1-related diseases and supports the need for better characterizing oligodendrocytes as actors playing a role in neurodegenerative diseases, also pointing at them as potential novel targets to set a brake to disease progression.
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- 2024
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15. The Limits of Limited Commitment
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Bizzotto, Jacopo, Hinnosaar, Toomas, and Vigier, Adrien
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Economics - Theoretical Economics - Abstract
We study limited strategic leadership. A collection of subsets covering the leader's action space determines her commitment opportunities. We characterize the outcomes resulting from all possible commitment structures of this kind. If the commitment structure is an interval partition, then the leader's payoff is bounded by her Stackelberg and Cournot payoffs. However, under more general commitment structures the leader may obtain a payoff that is less than her minimum Cournot payoff. We apply our results to study information design problems in leader-follower games where a mediator communicates information about the leader's action to the follower.
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- 2022
16. Gut microbiota depletion delays somatic peripheral nerve development and impairs neuromuscular junction maturation
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Matilde Cescon, Giovanna Gambarotta, Sonia Calabrò, Chiara Cicconetti, Francesca Anselmi, Svenja Kankowski, Luisa Lang, Marijana Basic, Andre Bleich, Silvia Bolsega, Matthias Steglich, Salvatore Oliviero, Stefania Raimondo, Dario Bizzotto, Kirsten Haastert-Talini, and Giulia Ronchi
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Germ-free mice ,gnotobiotic mice ,peripheral nerve development ,skeletal muscle ,myelin ,Schwann cells ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Gut microbiota is responsible for essential functions in human health. Several communication axes between gut microbiota and other organs via neural, endocrine, and immune pathways have been described, and perturbation of gut microbiota composition has been implicated in the onset and progression of an emerging number of diseases. Here, we analyzed peripheral nerves, dorsal root ganglia (DRG), and skeletal muscles of neonatal and young adult mice with the following gut microbiota status: a) germ-free (GF), b) gnotobiotic, selectively colonized with 12 specific gut bacterial strains (Oligo-Mouse-Microbiota, OMM12), or c) natural complex gut microbiota (CGM). Stereological and morphometric analyses revealed that the absence of gut microbiota impairs the development of somatic median nerves, resulting in smaller diameter and hypermyelinated axons, as well as in smaller unmyelinated fibers. Accordingly, DRG and sciatic nerve transcriptomic analyses highlighted a panel of differentially expressed developmental and myelination genes. Interestingly, the type III isoform of Neuregulin1 (NRG1), known to be a neuronal signal essential for Schwann cell myelination, was overexpressed in young adult GF mice, with consequent overexpression of the transcription factor Early Growth Response 2 (Egr2), a fundamental gene expressed by Schwann cells at the onset of myelination. Finally, GF status resulted in histologically atrophic skeletal muscles, impaired formation of neuromuscular junctions, and deregulated expression of related genes. In conclusion, we demonstrate for the first time a gut microbiota regulatory impact on proper development of the somatic peripheral nervous system and its functional connection to skeletal muscles, thus suggesting the existence of a novel ‘Gut Microbiota-Peripheral Nervous System-axis.’
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- 2024
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17. Salbutamol repurposing ameliorates neuromuscular junction defects and muscle atrophy in Col6a1−/− mouse model of collagen VI‐related myopathies
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Sonia Calabrò, Leonardo Nogara, Yongzhi Jian, Manuel Valentin, Dario Bizzotto, Paola Braghetta, Loris Russo, Lisa Gambarotto, Bert Blaauw, Said Hashemolhosseini, Paolo Bonaldo, and Matilde Cescon
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Medicine (General) ,R5-920 - Published
- 2024
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18. Variable positive end-expiratory pressure in an experimental model of acute respiratory distress syndrome: an advanced ventilation modality
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Südy, Roberta, Diaper, John, Bizzotto, Davide, Dellàca, Rafaelle, Petak, Ferenc, Habre, Walid, and Dos Santos Rocha, Andre
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- 2024
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19. Phytotherapy Might Have a Role in Reducing Unnecessary Prostate Biopsies: Results from an Exploratory, Randomized Controlled Trial of Two Different Phytotherapeutic Agents
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Tommaso Cai, Irene Tamanini, Marco Puglisi, Leonardo Bizzotto, Michele Rizzo, Giovanni Liguori, Luca Gallelli, Alessandro Palmieri, and Truls E. Bjerklund Johansen
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prostate-specific antigen ,prostate cancer ,diagnosis ,inflammation ,phytotherapy ,Medicine (General) ,R5-920 - Abstract
Background: We aimed to evaluate the impact of two different phytotherapeutic agents on decision making regarding prostate biopsy for patients with higher-than-normal prostate-specific antigen (PSA) levels. Methods: From June 2022 to May 2023, all patients attending two urological institutions due to higher-than-normal PSA levels were randomized to receive either oral capsules of Curcuma Longa, Boswellia, Pinus pinaster and Urtica dioica (Group A) or Serenoa Repens 320 mg (Group B) for 3 months. At the follow-up visit after 3 months, all patients underwent PSA tests and multiparametric magnetic resonance imaging (mpMRI). Results: In the per-protocol analysis, data from 66 patients in Group A and 76 in Group B were analyzed. Fifty patients in Group A (75.7%) showed a significant reduction in total PSA compared to forty-nine in Group B (64.4%) (p < 0.001). Twenty-eight patients had PI-RADS III or higher in mpMRI: twelve in Group A and fourteen in Group B. Twenty-three patients (34.8%) in Group A and fifty-nine (77.6%) in Group B (p < 0.001) underwent prostate biopsy based on the mpMRI findings and PSA values. Three patients in Group A showed a significant reduction in total PSA values while having positive mpMRI findings (6%) compared with nine in Group B (19.5%) (p < 0.001). On the contrary, 7 patients in Group A did not show significant reduction in total PSA values and had negative mpMRI findings (43%) compared to 22 in Group B (81.4%) (p < 0.001). Conclusions: In conclusion, a three-month course of a combination of Curcuma Longa, Boswellia, Pinus pinaster and Urtica dioica seems to be an interesting tool to avoid unnecessary prostate biopsies among men with higher-than-normal PSA levels.
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- 2024
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20. Reseña del Proyecto 'PANEX-Youth: Adaptações de jovens em comunidades vulneráveis para sobreviver e recuperar da Covid-19'
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Luciana Bizzotto, Yorman Paredes, Leonardo Musumeci, and Leandro Giatti
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Medicine (General) ,R5-920 ,Public aspects of medicine ,RA1-1270 - Published
- 2023
21. Galectin-1 Attenuates PDGF-Mediated AKT Signaling in Retinal Pigment Epithelial Cells
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Martina Bizzotto, Annabella Ostermaier, Caspar Liesenhoff, Wenxiu Ma, Arie Geerlof, Siegfried G. Priglinger, Claudia S. Priglinger, and Andreas Ohlmann
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galectin-1 ,galectin-1 deficiency ,AKT signaling ,pAKT ,ERK signaling ,pERK ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Galectins have the potential to interact with transmembrane glycoproteins to modulate their functions. Since galectin-1 interacts with PDGF-Rβ, we analyzed the effect of galectin-1 on PDGF-BB-mediated AKT signaling in primary human retinal pigment epithelial (RPE) cells and galectin-1-deficient immortalized human RPE cells (LGALS1−/−/ARPE-19) following incubation with PDGF-BB and galectin-1. Expression and localization of galectin-1, PDGF-Rβ and pAKT were investigated using western blot analysis and immunohistochemical staining. Cell proliferation of RPE cells was analyzed using BrdU ELISA. Following treatment of human RPE cells with human recombinant (hr)-galectin-1 and PDGF-BB, an intense clustering of PDGF-Rβ and colocalization with galectin-1 were detected. By Western blot analysis and immunocytochemistry of human RPE cells, an enhanced PDGF-BB-mediated expression of pAKT was observed, which was substantially reduced by additional incubation with hr-galectin-1. Vice versa, in LGALS1−/−/ARPE-19 cells, the PDGF-BB-induced pAKT signal was enhanced compared to wild-type cells. Furthermore, a decreased expression of PDGF-Rβ in human RPE cells was observed after treatment with PDGF-BB and hr-galectin-1, while in untreated LGALS1−/−/ARPE-19 cells, its constitutive expression was increased. In addition, after treatment of RPE cells with hr-galectin-1, the PDGF-BB-induced proliferation was markedly reduced. In summary, galectin-1 has the distinct potential to reduce PDGF-mediated pAKT signaling and proliferation in human RPE cells—an effect that is most likely facilitated via a decreased expression of PDGF-Rβ.
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- 2024
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22. Classification of bioactive peptides: A systematic benchmark of models and encodings
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Bizzotto, Edoardo, Zampieri, Guido, Treu, Laura, Filannino, Pasquale, Di Cagno, Raffaella, and Campanaro, Stefano
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- 2024
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23. Analysis of somatic mutations in 131 human brains reveals aging-associated hypermutability
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Bae, Taejeong, Fasching, Liana, Wang, Yifan, Shin, Joo Heon, Suvakov, Milovan, Jang, Yeongjun, Norton, Scott, Dias, Caroline, Mariani, Jessica, Jourdon, Alexandre, Wu, Feinan, Panda, Arijit, Pattni, Reenal, Chahine, Yasmine, Yeh, Rebecca, Roberts, Rosalinda C, Huttner, Anita, Kleinman, Joel E, Hyde, Thomas M, Straub, Richard E, Walsh, Christopher A, Urban, Alexander E, Leckman, James F, Weinberger, Daniel R, Vaccarino, Flora M, Abyzov, Alexej, Park, Peter J, Sestan, Nenad, Weinberger, Daniel, Moran, John V, Gage, Fred H, Gleeson, Joseph, Mathern, Gary, Courchesne, Eric, Roy, Subhojit, Chess, Andrew J, Akbarian, Schahram, Bizzotto, Sara, Coulter, Michael, D’Gama, Alissa, Ganz, Javier, Hill, Robert, Huang, August Yue, Khoshkhoo, Sattar, Kim, Sonia, Lee, Alice, Lodato, Michael, Maury, Eduardo A, Miller, Michael, Borges-Monroy, Rebeca, Rodin, Rachel, Zhou, Zinan, Bohrson, Craig, Chu, Chong, Cortes-Ciriano, Isidro, Dou, Yanmei, Galor, Alon, Gulhan, Doga, Kwon, Minseok, Luquette, Joe, Sherman, Maxwell, Viswanadham, Vinay, Jones, Attila, Rosenbluh, Chaggai, Cho, Sean, Langmead, Ben, Thorpe, Jeremy, Erwin, Jennifer, Jaffe, Andrew, McConnell, Michael, Narurkar, Rujuta, Paquola, Apua, Shin, Jooheon, Straub, Richard, Molitor, Cindy, Peters, Mette, Linker, Sara, Reed, Patrick, Wang, Meiyan, Urban, Alexander, Zhou, Bo, Zhu, Xiaowei, Serres Amero, Aitor, Juan, David, Lobon, Irene, Marques-Bonet, Tomas, Solis Moruno, Manuel, Garcia Perez, Raquel, Povolotskaya, Inna, Soriano, Eduardo, Antaki, Danny, and Averbuj, Dan
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Biotechnology ,Brain Disorders ,Intellectual and Developmental Disabilities (IDD) ,Genetics ,Autism ,Pediatric ,Mental Health ,Human Genome ,Underpinning research ,2.1 Biological and endogenous factors ,Aetiology ,1.1 Normal biological development and functioning ,Mental health ,Aging ,Autistic Disorder ,Brain ,Enhancer Elements ,Genetic ,Gene Expression Regulation ,Humans ,Mutagenesis ,Mutation ,Protein Binding ,Transcription Factors ,Whole Genome Sequencing ,Brain Somatic Mosaicism Network§ ,General Science & Technology - Abstract
We analyzed 131 human brains (44 neurotypical, 19 with Tourette syndrome, 9 with schizophrenia, and 59 with autism) for somatic mutations after whole genome sequencing to a depth of more than 200×. Typically, brains had 20 to 60 detectable single-nucleotide mutations, but ~6% of brains harbored hundreds of somatic mutations. Hypermutability was associated with age and damaging mutations in genes implicated in cancers and, in some brains, reflected in vivo clonal expansions. Somatic duplications, likely arising during development, were found in ~5% of normal and diseased brains, reflecting background mutagenesis. Brains with autism were associated with mutations creating putative transcription factor binding motifs in enhancer-like regions in the developing brain. The top-ranked affected motifs corresponded to MEIS (myeloid ectopic viral integration site) transcription factors, suggesting a potential link between their involvement in gene regulation and autism.
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- 2022
24. Presentation on the methodology used to calculate the self-rescuing index for communities downstream mining dams
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de Oliveira, Natália Maria, Bizzotto Gomes, Francisco, Gontijo Oliveira, Ewerton, Castelo Branco, Felipe, Junqueira, José Cláudio, Rocha Godinho, Beatriz, Santos, Bruno, and Batista, César
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- 2024
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25. Predicting prostate cancer progression with a Multi-lncRNA expression-based risk score and nomogram integrating ISUP grading
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Ledesma-Bazan, Sabrina, Cascardo, Florencia, Bizzotto, Juan, Olszevicki, Santiago, Vazquez, Elba, Gueron, Geraldine, and Cotignola, Javier
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- 2024
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26. The interaction of patients’ physical status and time to endoscopy on mortality risk in patients with upper gastrointestinal bleeding: A national prospective cohort study
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Amitrano, L, Anderloni, A, Andriulli, A, Annese, V, Baldassarre, G, Bargiggia, S, Balzano, A, Bazzoli, F, Bennato, R, Bianco, M A, Bizzotto, A, Boarino, V, Bonanomi, AG, Borgheresi, P, Bresci, G, Buffoli, F, Buscarini, E, Castrignanò, G, Cavallaro, LG, Cesaro, P, Chirico, A, Cipolletta, F, Cipolletta, L, Conigliaro, R, Conte, D, Costamagna, G, Covello, F, D'Amico, G, De Fanis, C, De Filippo, FR, de Franchis, R, Dell‘Era, A, De Nigris, F, De Matthaeis, M, Di Giorgio, P, Di Giulio, E, Esposito, P, Ferraris, L, Filippino, A, Franceschi, M, Furio, L, Germana’, B, Grassia, R, Imperiali, G, Lamanda, R, Lauri, A, Londoni, C, Mangiafico, S, Manno, M, Marmo, C, Merighi, A, Meroni, R, Metrangolo, S, Montalbano, L M, Napolitano, G, Nucci, A, Orsini, L, Parente, F, Parravicini, M, Paterlini, A, Pumpo, R, Purita, L, Repici, A, Riccioni, ME, Russo, A, Segato, S, Sorrentino, I, Spinzi, G, Spotti, D, Tortora, A, Tomba, C, Triossi, O, Zagari, RM, Zambelli, A, Bucci, Cristina, Marmo, Clelia, Soncini, Marco, Riccioni, Maria Elena, Laursen, Stig B., Gralnek, Ian M., and Marmo, Riccardo
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- 2024
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27. Uptake of cyclic C6O4 in maize and tomato: Results from a greenhouse study
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Bizzotto, Elisa C., Libralato, Giovanni, de Natale, Antonino, Scanferla, Petra, Vighi, Marco, and Marcomini, Antonio
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- 2024
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28. Buffering against exposure to mental health misinformation in online communities on Facebook: the interplay of depression literacy and expert moderation
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Bizzotto, Nicole, de Bruijn, Gert-Jan, and Schulz, Peter Johannes
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- 2023
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29. CBD hydroxyquinone photo-isomerises to a highly reactive intermediate
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Thomson, Brodie. J., Hanna, Summer, Schwarzenberg, Adrian, Kiani, Pirouz, Bizzotto, Dan, Kennepohl, Pierre, Davies, Ashley, Roggen, Markus, and Sammis, Glenn M.
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- 2023
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30. Childhood obesity inequality in northeast China: joint effect of social economic status and school neighborhood environment
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Liu, Yang, Trude, Angela Cristina Bizzotto, Song, Shenzhi, Jiang, Nan, Wang, Shihan, Gittelsohn, Joel, and Wen, Deliang
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- 2023
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31. Normal meninges harbor oncogenic somatic mutations in meningioma-driver genes
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Boetto, Julien, Plu, Isabelle, Ducos, Yohan, Blouin, Antoine, Teranishi, Yu, Bizzotto, Sara, Kalamarides, Michel, and Peyre, Matthieu
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- 2023
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32. Buffering against exposure to mental health misinformation in online communities on Facebook: the interplay of depression literacy and expert moderation
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Nicole Bizzotto, Gert-Jan de Bruijn, and Peter Johannes Schulz
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Online communities ,Misinformation ,Mental health ,Depression literacy ,Expert moderation ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The proliferation of health misinformation on social media is a growing public health concern. Online communities for mental health (OCMHs) are also considered an outlet for exposure to misinformation. This study explored the impact of the self-reported volume of exposure to mental health misinformation in misinformation agreement and the moderating effects of depression literacy and type of OCMHs participation (expert vs. peer-led). Methods Participants (n = 403) were recruited in Italian-speaking OCMHs on Facebook. We conducted regression analyses using PROCESS macro (moderated moderation, Model 3). Measures included: the Depression Literacy Questionnaire (Griffiths et al., 2004), the self-reported misinformation exposure in the OCMHs (3 items), and misinformation agreement with the exposure items (3 items). Whether participants were members of expert or peer-led OCMHs was also investigated. Results The final model explained the 12% variance in the agreement. There was a positive and significant relationship between misinformation exposure and misinformation agreement (β = 0.3221, p
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- 2023
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33. Contrasting somatic mutation patterns in aging human neurons and oligodendrocytes
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Ganz, Javier, Luquette, Lovelace J., Bizzotto, Sara, Miller, Michael B., Zhou, Zinan, Bohrson, Craig L., Jin, Hu, Tran, Antuan V., Viswanadham, Vinayak V., McDonough, Gannon, Brown, Katherine, Chahine, Yasmine, Chhouk, Brian, Galor, Alon, Park, Peter J., and Walsh, Christopher A.
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- 2024
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34. Cytokine mRNA expression in the bronchoalveolar lavage cells from horses affected by different equine asthma subtypes
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Lo Feudo, Chiara Maria, Stucchi, Luca, Bazzocchi, Chiara, Consiglio, Anna Lange, Comazzi, Stefano, Cozzi, Maria Cristina, Gusmara, Claudia, Gaspari, Giulia, Cialini, Chiara, Bizzotto, Davide, Dellacà, Raffaele, and Ferrucci, Francesco
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- 2024
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35. Toxicity and bioaccumulation of the fluorosurfactant cC6O4 in the earthworm Eisenia foetida (Savigny, 1826)
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Bizzotto, Elisa Chiara, Libralato, Giovanni, Breda, Silvia, Siciliano, Antonietta, Scanferla, Petra, Vighi, Marco, and Marcomini, Antonio
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- 2024
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36. Fate and effects of a new generation fluorosurfactant (cC6O4) in freshwater mesocosms
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Rico, Andreu, Crettaz-Minaglia, Melina, García-Astillero, Ariadna, Bizzotto, Elisa, and Vighi, Marco
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- 2024
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37. Comprehensive identification of somatic nucleotide variants in human brain tissue
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Wang, Yifan, Bae, Taejeong, Thorpe, Jeremy, Sherman, Maxwell A, Jones, Attila G, Cho, Sean, Daily, Kenneth, Dou, Yanmei, Ganz, Javier, Galor, Alon, Lobon, Irene, Pattni, Reenal, Rosenbluh, Chaggai, Tomasi, Simone, Tomasini, Livia, Yang, Xiaoxu, Zhou, Bo, Akbarian, Schahram, Ball, Laurel L, Bizzotto, Sara, Emery, Sarah B, Doan, Ryan, Fasching, Liana, Jang, Yeongjun, Juan, David, Lizano, Esther, Luquette, Lovelace J, Moldovan, John B, Narurkar, Rujuta, Oetjens, Matthew T, Rodin, Rachel E, Sekar, Shobana, Shin, Joo Heon, Soriano, Eduardo, Straub, Richard E, Zhou, Weichen, Chess, Andrew, Gleeson, Joseph G, Marquès-Bonet, Tomas, Park, Peter J, Peters, Mette A, Pevsner, Jonathan, Walsh, Christopher A, Weinberger, Daniel R, Vaccarino, Flora M, Moran, John V, Urban, Alexander E, Kidd, Jeffrey M, Mills, Ryan E, and Abyzov, Alexej
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Biological Sciences ,Bioinformatics and Computational Biology ,Genetics ,Neurosciences ,Human Genome ,1.1 Normal biological development and functioning ,Underpinning research ,Generic health relevance ,Alleles ,Brain ,Chromosome Mapping ,Computational Biology ,Genetic Association Studies ,Genetic Variation ,Genomics ,Germ Cells ,High-Throughput Nucleotide Sequencing ,Humans ,Organ Specificity ,Polymorphism ,Single Nucleotide ,Brain Somatic Mosaicism Network ,Environmental Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
BackgroundPost-zygotic mutations incurred during DNA replication, DNA repair, and other cellular processes lead to somatic mosaicism. Somatic mosaicism is an established cause of various diseases, including cancers. However, detecting mosaic variants in DNA from non-cancerous somatic tissues poses significant challenges, particularly if the variants only are present in a small fraction of cells.ResultsHere, the Brain Somatic Mosaicism Network conducts a coordinated, multi-institutional study to examine the ability of existing methods to detect simulated somatic single-nucleotide variants (SNVs) in DNA mixing experiments, generate multiple replicates of whole-genome sequencing data from the dorsolateral prefrontal cortex, other brain regions, dura mater, and dural fibroblasts of a single neurotypical individual, devise strategies to discover somatic SNVs, and apply various approaches to validate somatic SNVs. These efforts lead to the identification of 43 bona fide somatic SNVs that range in variant allele fractions from ~ 0.005 to ~ 0.28. Guided by these results, we devise best practices for calling mosaic SNVs from 250× whole-genome sequencing data in the accessible portion of the human genome that achieve 90% specificity and sensitivity. Finally, we demonstrate that analysis of multiple bulk DNA samples from a single individual allows the reconstruction of early developmental cell lineage trees.ConclusionsThis study provides a unified set of best practices to detect somatic SNVs in non-cancerous tissues. The data and methods are freely available to the scientific community and should serve as a guide to assess the contributions of somatic SNVs to neuropsychiatric diseases.
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- 2021
38. Minimum curvature path planning for a dual stage positioning system*.
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Marco Monte, Roberto Oboe, Emanuele Siego, Davide Pilastro, and Stefano Bizzotto
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- 2023
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39. Trem2 expression in microglia is required to maintain normal neuronal bioenergetics during development
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Tagliatti, Erica, Desiato, Genni, Mancinelli, Sara, Bizzotto, Matteo, Gagliani, Maria C., Faggiani, Elisa, Hernández-Soto, Rebeca, Cugurra, Andrea, Poliseno, Paola, Miotto, Matteo, Argüello, Rafael J., Filipello, Fabia, Cortese, Katia, Morini, Raffaella, Lodato, Simona, and Matteoli, Michela
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- 2024
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40. FEEDS, the Food wastE biopEptiDe claSsifier: From microbial genomes and substrates to biopeptides function
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Centurion, Victor Borin, Bizzotto, Edoardo, Tonini, Stefano, Filannino, Pasquale, Di Cagno, Raffaella, Zampieri, Guido, and Campanaro, Stefano
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- 2024
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41. FRET Imaging of Nonuniformly Distributed DNA SAMs on Gold Reveals the Role Played by the Donor/Acceptor Ratio and the Local Environment in Measuring the Rate of Hybridization
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Adrian Jan Grzędowski, Tianxiao Ma, and Dan Bizzotto
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Medical technology ,R855-855.5 ,Chemistry ,QD1-999 - Abstract
Mixed DNA SAMs labeled with a fluorophore (either AlexaFluor488 or AlexaFluor647) were prepared on a single crystal gold bead electrode using potential-assisted thiol exchange and studied using Förster resonance energy transfer (FRET). A measure of the local environment of the DNA SAM (e.g., crowding) was possible using FRET imaging on these surfaces since electrodes prepared this way have a range of surface densities (ΓDNA). The FRET signal was strongly dependent on ΓDNA and on the ratio of AlexaFluor488 to AlexaFluor647 used to make the DNA SAM, which were consistent with a model of FRET in 2D systems. FRET was shown to provide a direct measure of the local DNA SAM arrangement on each crystallographic region of interest providing a direct assessment of the probe environment and its influence on the rate of hybridization. The kinetics of duplex formation for these DNA SAMs was also studied using FRET imaging over a range of coverages and DNA SAM compositions. Hybridization of the surface-bound DNA increased the average distance between the fluorophore label and the gold electrode surface and decreased the distance between the donor (D) and acceptor (A), both of which result in an increase in FRET intensity. This increase in FRET was modeled using a second order Langmuir adsorption rate equation, reflecting the fact that both D and A labeled DNA are required to become hybridized to observe a FRET signal. The self-consistent analysis of the hybridization rates on low and high coverage regions on the same electrode showed that the low coverage regions achieved full hybridization 5× faster than the higher coverage regions, approaching rates typically found in solution. The relative increase in FRET intensity from each region of interest was controlled by manipulating the donor to acceptor composition of the DNA SAM without changing the rate of hybridization. The FRET response can be optimized by controlling the coverage and the composition of the DNA SAM sensor surface and could be further improved with the use of a FRET pair with a larger (e.g., > 5 nm) Förster radius.
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- 2023
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42. FEEDS, the Food wastE biopEptiDe claSsifier: From microbial genomes and substrates to biopeptides function
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Victor Borin Centurion, Edoardo Bizzotto, Stefano Tonini, Pasquale Filannino, Raffaella Di Cagno, Guido Zampieri, and Stefano Campanaro
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Bioactive peptides ,Proteases ,Digestion ,Food waste ,Bacteria ,Yeasts ,Biotechnology ,TP248.13-248.65 - Abstract
The production of biopeptides from food waste through microbial fermentation faces challenges arising from the diverse proteolytic abilities of microorganisms and substrate variability, impacting both the quality and yield of generated biopeptides. To address these challenges, preliminary in-silico bioinformatics analyses play a crucial role in evaluating suitable substrates and proteases for the fermentation process. However, existing tools lack comprehensive predictive capabilities for relevant proteases, substrate performance assessment, and final biopeptide family characterization. To overcome these limitations, we developed FEEDS (Food wastE biopEptiDe claSsifier), a novel biopeptide prediction and classification tool. FEEDS predicts biopeptide families based on microbial genome protease profiles and substrate composition during proteolysis. The tool also employs a machine learning approach for functional biopeptide classification. Results from testing on 1000 microbial genomes demonstrate the effectiveness of biopeptide classification, particularly in categorizing peptides derived from substrates like Hordeum vulgare and Vitis vinifera seed storage proteins. In addition to biopeptide classification, our study delves into the distinctive protease profiles of bacteria and yeast genomes. Bacterial genomes exhibited 60 to 100 proteases across 40–55 families. Contrastingly, yeast genomes displayed a more evenly distributed pattern with 150 to 160 protease-encoding genes across 60 to 67 families, surpassing bacterial counts. Remarkably, a substantial portion of yeast proteases (∼66 %) was secreted. Moreover, our integration of a machine learning methodology within the FEEDS pipeline proved highly effective, achieving over 80 % accuracy in predicting the function of peptides derived from seed storage proteins. Notably, longer peptide sequences exceeding 20 amino acids consistently displayed a higher probability of correct assignment compared to shorter counterparts.
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- 2024
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43. Holistic Multi-scale Imaging of Oxygen Reduction Reaction Catalyst Degradation in Operational Fuel Cells
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Martens, Isaac, Vamvakeros, Antonis, Martinez, Nicolas, Chattot, Raphaël, Pusa, Janne, Blanco, Maria Valeria, Fisher, Elizabeth A., Asset, Tristan, Escribano, Sylvie, Micoud, Fabrice, Starr, Tim, Coelho, Alan, Honkimäki, Veijo, Bizzotto, Dan, Wilkinson, David P., Jacques, Simon D. M., Maillard, Frédéric, Dubau, Laetitia, Lyonnard, Sandrine, Morin, Arnaud, and Drnec, Jakub
- Subjects
Physics - Applied Physics ,Condensed Matter - Materials Science - Abstract
Wide proliferation of low temperature hydrogen fuel cell systems, a key part of the hydrogen economy, is hindered by degradation of the platinum cathode catalyst. Here, we provide a device level assessment of the molecular scale catalyst degradation phenomena, using advanced operando X-ray scattering tomography tailored for device-scale imaging. Each cell component, including the catalyst, carbon support, polymer electrolyte, and liquid water can be simultaneously mapped, allowing for deep correlative analysis. Chemical and thermal gradients formed inside the operating fuel cell produce highly heterogeneous degradation of the catalyst nanostructure, which can be linked to the macroscale design of the flow field and water distribution in the cell materials. Striking differences in catalyst degradation are observed between operating fuel cell devices and the liquid cell routinely used for catalyst stability studies, highlighting the rarely studied but crucial impact of the complex operating environment on the catalyst degradation phenomena. This degradation knowledge gap highlights the necessity of multimodal in situ characterization of real devices when assessing the performance and durability of electrocatalysts., Comment: 63 pages, 40 figures
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- 2020
44. Landmarks of human embryonic development inscribed in somatic mutations
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Bizzotto, Sara, Dou, Yanmei, Ganz, Javier, Doan, Ryan N, Kwon, Minseok, Bohrson, Craig L, Kim, Sonia N, Bae, Taejeong, Abyzov, Alexej, Network†, NIMH Brain Somatic Mosaicism, Park, Peter J, and Walsh, Christopher A
- Subjects
Biological Sciences ,Bioinformatics and Computational Biology ,Biomedical and Clinical Sciences ,Genetics ,Human Genome ,Pediatric ,Biotechnology ,Stem Cell Research - Nonembryonic - Non-Human ,Stem Cell Research ,2.1 Biological and endogenous factors ,Aetiology ,Underpinning research ,1.1 Normal biological development and functioning ,Generic health relevance ,Adolescent ,Adult ,Cell Division ,Cell Lineage ,Clone Cells ,Embryonic Development ,Female ,Gastrula ,Gastrulation ,Genetic Variation ,Germ Layers ,Humans ,Male ,Mutation ,Neural Stem Cells ,Neurons ,Organogenesis ,Polymorphism ,Single Nucleotide ,Prosencephalon ,Single-Cell Analysis ,Whole Genome Sequencing ,NIMH Brain Somatic Mosaicism Network ,General Science & Technology - Abstract
Although cell lineage information is fundamental to understanding organismal development, very little direct information is available for humans. We performed high-depth (250×) whole-genome sequencing of multiple tissues from three individuals to identify hundreds of somatic single-nucleotide variants (sSNVs). Using these variants as "endogenous barcodes" in single cells, we reconstructed early embryonic cell divisions. Targeted sequencing of clonal sSNVs in different organs (about 25,000×) and in more than 1000 cortical single cells, as well as single-nucleus RNA sequencing and single-nucleus assay for transposase-accessible chromatin sequencing of ~100,000 cortical single cells, demonstrated asymmetric contributions of early progenitors to extraembryonic tissues, distinct germ layers, and organs. Our data suggest onset of gastrulation at an effective progenitor pool of about 170 cells and about 50 to 100 founders for the forebrain. Thus, mosaic mutations provide a permanent record of human embryonic development at very high resolution.
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- 2021
45. CBD hydroxyquinone photo-isomerises to a highly reactive intermediate
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Brodie. J. Thomson, Summer Hanna, Adrian Schwarzenberg, Pirouz Kiani, Dan Bizzotto, Pierre Kennepohl, Ashley Davies, Markus Roggen, and Glenn M. Sammis
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Medicine ,Science - Abstract
Abstract The legalisation of hemp has led to wide commercial availability of cannabidiol (CBD)-containing products. Here we show that the CBD-hydroxyquinone (HU-331), a readily formed oxidation product and common impurity in CBD isolates, undergoes a previously unknown photo-isomerisation to produce a highly reactive intermediate in solution. Studies supported by calculations indicate that this intermediate rapidly reacts with oxygen to form a multitude of cannabinoid products. The purple colour observed in light-aged CBD-containing solutions is largely due to the anions of these by-products and is not significantly due to the HU-331 anion. Our findings suggest that these uncharacterized cannabinoid derivatives can be present in CBD-containing e-liquids and solutions that have been stored under ambient light conditions, calling for quality control processes that manage HU-331 contamination.
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- 2023
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46. Childhood obesity inequality in northeast China: joint effect of social economic status and school neighborhood environment
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Yang Liu, Angela Cristina Bizzotto Trude, Shenzhi Song, Nan Jiang, Shihan Wang, Joel Gittelsohn, and Deliang Wen
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Childhood obesity ,Social economic status ,Neighborhood environment ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Obesogenic environment is important in driving obesity epidemic. Children spend large amount of their time in schools. School neighborhood environment, as well as its interaction with socioeconomic status (SES) needs to be explored to provide evidence for children obesity prevention policies. Methods Objective anthropometric measurement, a household structured questionnaire, and school geospatial analyses were carried out on 3670 children (aged 9–12 years) of 26 schools in northeast China. Interaction between SES inter-categorical intersectionality group and school neighborhood environment was tested for the effect on children’s body mass index z scores (z-BMI) and waist–hip ratio z scores (z-WHR), following formulation of SES inter-categorical intersectionality group based on household wealth, parental education, and parental occupation. Results SES groups formed by household wealth, parental education and parental occupation was associated with z-BMI and z-WHR for girls. Those from moderate wealth & self-employed (M&S) families had the highest adjusted z-BMI and z-WHR among all SES groups. School neighborhood environment factors interacted with SES groups in association with WHR for girls. Number of school neighborhood supermarkets and residential sites were negatively associated with z-WHR for girls from M&S families (β= -0.45 (95%CI: -0.76, -0.15) for supermarkets; β= -0.01 (95%CI: -0.03, 0.00) for residential sites). Number of school neighborhood convenience stores and public transport stops were positively associated with z-WHR for girls from M&S families (β = 0.02 (95%CI: 0.00, 0.03) for convenience stores; β = 0.23 (95%CI: 0.15, 0.31) for public transport stops). While non-significant association was found for number of vegetable stores. Conclusion Girls from moderate wealth & self-employed families may be the group susceptible to school neighborhood environment. Local policies targeted at improving the school neighborhood environment may be one avenue for reducing socioeconomic disparities in obesity especially for girls.
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- 2023
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47. Modification of NMC811 with titanium for enhanced cycling and high-voltage stability
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Bizzotto, Francesco, Dachraoui, Walid, Grissa, Rabeb, Zhao, Wengao, Pagani, Francesco, Querel, Edouard, Kühnel, Ruben-Simon, and Battaglia, Corsin
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- 2023
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48. Schizophrenia-associated somatic copy-number variants from 12,834 cases reveal recurrent NRXN1 and ABCB11 disruptions
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Marshall, Christian R., Merico, Daniele, Thiruvahindrapuram, Bhooma, Wang, Zhouzhi, Scherer, Stephen W., Howrigan, Daniel P, Ripke, Stephan, Bulik-Sullivan, Brendan, Farh, Kai-How, Fromer, Menachem, Goldstein, Jacqueline I., Huang, Hailiang, Lee, Phil, Daly, Mark J., Neale, Benjamin M., Belliveau, Richard A., Jr., Bergen, Sarah E., Bevilacqua, Elizabeth, Chambert, Kimberley D., O'Dushlaine, Colm, Scolnick, Edward M., Smoller, Jordan W., Moran, Jennifer L., Palotie, Aarno, Petryshen, Tracey L., Wu, Wenting, Greer, Douglas S., Antaki, Danny, Shetty, Aniket, Gujral, Madhusudan, Brandler, William M., Malhotra, Dheeraj, Fuentes Fajarado, Karin V., Maile, Michelle S., Holmans, Peter A., Carrera, Noa, Craddock, Nick, Escott-Price, Valentina, Georgieva, Lyudmila, Hamshere, Marian L., Kavanagh, David, Legge, Sophie E., Pocklington, Andrew J., Richards, Alexander L., Ruderfer, Douglas M., Williams, Nigel M., Kirov, George, Owen, Michael J., Pinto, Dalila, Cai, Guiqing, Davis, Kenneth L., Drapeau, Elodie, Friedman, Joseph I, Haroutunian, Vahram, Parkhomenko, Elena, Reichenberg, Abraham, Silverman, Jeremy M., Buxbaum, Joseph D., Domenici, Enrico, Agartz, Ingrid, Djurovic, Srdjan, Mattingsdal, Morten, Melle, Ingrid, Andreassen, Ole A., Jönsson, Erik G., Söderman, Erik, Albus, Margot, Alexander, Madeline, Laurent, Claudine, Levinson, Douglas F., Amin, Farooq, Atkins, Joshua, Cairns, Murray J., Scott, Rodney J., Tooney, Paul A., Wu, Jing Qin, Bacanu, Silviu A., Bigdeli, Tim B., Reimers, Mark A., Webb, Bradley T., Wolen, Aaron R., Wormley, Brandon K., Kendler, Kenneth S., Riley, Brien P., Kähler, Anna K., Magnusson, Patrik K.E., Hultman, Christina M., Bertalan, Marcelo, Hansen, Thomas, Olsen, Line, Rasmussen, Henrik B., Werge, Thomas, Mattheisen, Manuel, Black, Donald W., Bruggeman, Richard, Buccola, Nancy G., Buckner, Randy L., Roffman, Joshua L., Byerley, William, Cahn, Wiepke, Kahn, René S, Strengman, Eric, Ophoff, Roel A., Carr, Vaughan J., Catts, Stanley V., Henskens, Frans A., Loughland, Carmel M., Michie, Patricia T., Pantelis, Christos, Schall, Ulrich, Jablensky, Assen V., Kelly, Brian J., Campion, Dominique, Cantor, Rita M., Cheng, Wei, Cloninger, C. Robert, Svrakic, Dragan M, Cohen, David, Cormican, Paul, Donohoe, Gary, Morris, Derek W., Corvin, Aiden, Gill, Michael, Crespo-Facorro, Benedicto, Crowley, James J., Farrell, Martilias S., Giusti-Rodríguez, Paola, Kim, Yunjung, Szatkiewicz, Jin P., Williams, Stephanie, Curtis, David, Pimm, Jonathan, Gurling, Hugh, McQuillin, Andrew, Davidson, Michael, Weiser, Mark, Degenhardt, Franziska, Forstner, Andreas J., Herms, Stefan, Hoffmann, Per, Hofman, Andrea, Cichon, Sven, Nöthen, Markus M., Del Favero, Jurgen, DeLisi, Lynn E., McCarley, Robert W., Levy, Deborah L., Mesholam-Gately, Raquelle I., Seidman, Larry J., Dikeos, Dimitris, Papadimitriou, George N., Dinan, Timothy, Duan, Jubao, Sanders, Alan R., Gejman, Pablo V., Gershon, Elliot S., Dudbridge, Frank, Eichhammer, Peter, Eriksson, Johan, Salomaa, Veikko, Essioux, Laurent, Fanous, Ayman H., Knowles, James A., Pato, Michele T., Pato, Carlos N., Frank, Josef, Meier, Sandra, Schulze, Thomas G., Strohmaier, Jana, Witt, Stephanie H., Rietschel, Marcella, Franke, Lude, Karjalainen, Juha, Freedman, Robert, Olincy, Ann, Freimer, Nelson B., Purcell, Shaun M., Roussos, Panos, Stahl, Eli A., Sklar, Pamela, Giegling, Ina, Hartmann, Annette M., Konte, Bettina, Rujescu, Dan, Godard, Stephanie, Hirschhorn, Joel N., Pers, Tune H., Price, Alkes, Esko, Tõnu, Gratten, Jacob, Lee, S. Hong, Visscher, Peter M., Wray, Naomi R., Mowry, Bryan J., de Haan, Lieuwe, Meijer, Carin J., Hansen, Mark, Ikeda, Masashi, Iwata, Nakao, Joa, Inge, Kalaydjieva, Luba, Keller, Matthew C., Kennedy, James L., Zai, Clement C., Knight, Jo, Lerer, Bernard, Liang, Kung-Yee, Lieberman, Jeffrey, Stroup, T. Scott, Lönnqvist, Jouko, Suvisaari, Jaana, Maher, Brion S., Maier, Wolfgang, Mallet, Jacques, McDonald, Colm, McIntosh, Andrew M., Blackwood, Douglas H.R., Metspalu, Andres, Milani, Lili, Milanova, Vihra, Mokrab, Younes, Collier, David A., Müller-Myhsok, Bertram, Murphy, Kieran C., Murray, Robin M., Powell, John, Myin-Germeys, Inez, Van Os, Jim, Nenadic, Igor, Nertney, Deborah A., Nestadt, Gerald, Pulver, Ann E., Nicodemus, Kristin K., Nisenbaum, Laura, Nordin, Annelie, Adolfsson, Rolf, O'Callaghan, Eadbhard, Oh, Sang-Yun, O'Neill, F. Anthony, Paunio, Tiina, Pietiläinen, Olli, Perkins, Diana O., Quested, Digby, Savitz, Adam, Li, Qingqin S., Schwab, Sibylle G., Shi, Jianxin, Spencer, Chris C.A., Thirumalai, Srinivas, Veijola, Juha, Waddington, John, Walsh, Dermot, Wildenauer, Dieter B., Bramon, Elvira, Darvasi, Ariel, Posthuma, Danielle, St. Clair, David, Shanta, Omar, Klein, Marieke, Park, Peter J., Weinberger, Daniel, Moran, John V., Gage, Fred H., Vaccarino, Flora M., Gleeson, Joseph, Mathern, Gary, Courchesne, Eric, Roy, Subhojit, Bizzotto, Sara, Coulter, Michael, Dias, Caroline, D'Gama, Alissa, Ganz, Javier, Hill, Robert, Huang, August Yue, Khoshkhoo, Sattar, Kim, Sonia, Lodato, Michael, Miller, Michael, Borges-Monroy, Rebeca, Rodin, Rachel, Zhou, Zinan, Bohrson, Craig, Chu, Chong, Cortes-Ciriano, Isidro, Dou, Yanmei, Galor, Alon, Gulhan, Doga, Kwon, Minseok, Luquette, Joe, Viswanadham, Vinay, Jones, Attila, Rosenbluh, Chaggai, Cho, Sean, Langmead, Ben, Thorpe, Jeremy, Erwin, Jennifer, Jaffe, Andrew, McConnell, Michael, Narurkar, Rujuta, Paquola, Apua, Shin, Jooheon, Straub, Richard, Abyzov, Alexej, Bae, Taejeong, Jang, Yeongjun, Wang, Yifan, Gage, Fred, Linker, Sara, Reed, Patrick, Wang, Meiyan, Urban, Alexander, Zhou, Bo, Zhu, Xiaowei, Pattni, Reenal, Amero, Aitor Serres, Juan, David, Lobon, Irene, Marques-Bonet, Tomas, Moruno, Manuel Solis, Perez, Raquel Garcia, Povolotskaya, Inna, Soriano, Eduardo, Averbuj, Dan, Ball, Laurel, Breuss, Martin, Yang, Xiaoxu, Chung, Changuk, Emery, Sarah B., Flasch, Diane A., Kidd, Jeffrey M., Kopera, Huira C., Kwan, Kenneth Y., Mills, Ryan E., Moldovan, John B., Sun, Chen, Zhao, Xuefang, Zhou, Weichen, Frisbie, Trenton J., Cherskov, Adriana, Fasching, Liana, Jourdon, Alexandre, Pochareddy, Sirisha, Scuderi, Soraya, Sestan, Nenad, Maury, Eduardo A., Sherman, Maxwell A., Genovese, Giulio, Gilgenast, Thomas G., Kamath, Tushar, Burris, S.J., Rajarajan, Prashanth, Flaherty, Erin, Akbarian, Schahram, Chess, Andrew, McCarroll, Steven A., Loh, Po-Ru, Phillips-Cremins, Jennifer E., Brennand, Kristen J., Macosko, Evan Z., Walters, James T.R., O’Donovan, Michael, Sullivan, Patrick, Sebat, Jonathan, Lee, Eunjung A., and Walsh, Christopher A.
- Published
- 2023
- Full Text
- View/download PDF
49. Measuring Implicit Gender Stereotypes Using the Preschool Auditory Stroop
- Author
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Gonzalez, Antonya Marie, Block, Katharina, Oh, Hee Jae Julie, Bizzotto, Riley, and Baron, Andrew Scott
- Abstract
Numerous studies suggest that by elementary school, children have implicit and explicit gender stereotypes about the toys, activities, roles, and abilities associated with boys vs. girls. Furthermore, these stereotypes have been shown to affect children's goals and behaviors, leading them to pursue activities that are associated with their own gender and avoid those that are not. The majority of previous research examining the development of children's implicit gender stereotypes has used the Implicit Association Test, which measures two associations simultaneously. Thus, it is often unclear which association is driving children's gender stereotypes, which hampers the ability to effectively target harmful associations for bias change. The current research uses the Preschool Auditory Stroop, an adaptation of the Auditory Stroop, to measure distinct implicit gender stereotypes in three to seven-year-old children. Across two studies, the first using human voices and the second using computer-generated voices, children were faster to respond when female-stereotypical words were paired with female voices and male-stereotypical words were paired with male voices. These results indicate that children have implicit gender stereotypes as early as age three. Furthermore, results suggested that the magnitude of these implicit gender stereotypes was comparable across our age range. This study indicates that implicit gender stereotypes are present in children as young as three, and results suggest that this methodology can be used in future research to chart the trajectory of distinct implicit gender stereotypes across development.
- Published
- 2022
- Full Text
- View/download PDF
50. Investigation of the critical specific gravity for cables and pipelines during various backfilling and installation processes
- Author
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Bizzotto, Tommaso, Brown, Michael, Brennan, Andrew, Powell, Toby, and Chandler, Howard W.
- Subjects
624.1 ,pipeline flotation ,uplift ,cable ,backfill plough ,drag coefficient ,Fine grained soils ,flotation ,granular material ,jet trencher ,mobilisation ,Pipeline installation ,pipeline ,ploughs ,trenching ,undrained shear strength - Abstract
Embedment of pipeline and cables in the seabed is achieved to protect them from issues such as loading by currents, damage from fishing trawler vessels and to provide thermal insulation. Depending on the type of soil and on financial and operational constraints, the embedment of pipelines/cables can be achieved using either ploughing or jet trenching with remotely operated vehicles. In this thesis the issues arising while embedding pipelines and cables using jet trenching and backfill ploughing were investigated by means of scaled physical model tests. The purpose of the investigation was to optimise the specific gravity (SG) of pipelines and cables to minimise flotation or movement during installation. The issues under considerations were the flotation of pipelines/cables in high moisture content fine grained materials due to jet trenching and the uplift of pipelines/cables due to backfilling in granular materials at different speeds. The effect of embedment depth and trench width on the soil resistance to the flotation of pipelines/cables were studied. Two types of test, free flotation and pull-out tests were used for validation at two different model scales. To summarise the results in a unified framework a relationship between undrained shear strength and liquidity index was calibrated using two fine grained materials at very high moisture contents. While the existing literature lacks a criterion to define flotation, a standard framework to quantify the mobilised resistance as a function of the movement of the pipeline/cable was developed to represent uplift displacement due to flotation. This was required as no abrupt flotation event actually occurred. The pipeline typically moved and then stopped as greater soil resistance was mobilised. This allows customisation of the flotation criterion to different industry needs (i.e. varying the allowable pipeline or cable displacement). Design charts and tools are provided to estimate undrained shear strength at high moisture content and resistance provided by soils at different embedment depth and trench width. These can be used to determine the lowest safe SG of pipelines/cables which will not exceed the maximum allowable movement. The effects of backfilling velocity on soil and pipeline or cable movement and the forces exerted by the soil on the pipeline/cable were also studied in separate testing. Two underwater plain strain model tests, at 7.5th and 50th scale, were employed to verify the scalability of the results. The effect of backfill ploughing in granular material was investigated using accelerometers and pore pressure transducers to quantify the magnitude and duration of forces on the model pipes. The soil flow behaviour and mechanisms were monitored via high speed video recording. The aim of the tests was to provide characterisation of the backfilling mechanism and quantify the forces acting on the pipe, whilst investigating their velocity dependency. The type of backfilling mechanism and the force acting on the pipe were found to be dependent on the backfilling velocity and scalable via the Froude number (Fr) calculated using plough velocity and pipe diameter. The changing point was found to be at Fr=0.1. The pore pressure measurements confirmed the dependency on the ploughing velocity of both the backfilling mechanism and the forces acting on the embedded product. Results from the model backfill ploughing tests were interpreted and used to calibrate an empirical correlation of upward forces as a function of backfilling velocity. The results of the pore pressure instrumentation were used to predict the duration of the uplift force action. These correlations allow the use of the model test results to determine the mechanical properties of pipelines/cables required to achieve correct embedment depth during installation to minimise movement during installation. Estimation of the most influential mechanical properties of pipelines/cables being installed is provided via simple structural analysis implementing Euler beam equation to represent the pipepline/cable being backfilled.
- Published
- 2020
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