1. Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines
- Author
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Marko Popovic, Amiram Grinvald, Brian M. Salzberg, Srdjan D. Antic, Knut Holthoff, Marco Canepari, Dejan Zecevic, Kaspar E. Vogt, Arthur Konnerth, Canepari, Marco, Canepari, M, Zecevic, D, ANTE-INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies, Division of Pharmacology and Neurobiology, Biozentrum [Basel, Suisse], University of Basel (Unibas)-University of Basel (Unibas)-Biozentrum [Basel, Suisse], University of Basel (Unibas)-University of Basel (Unibas), Department of Cellular and Molecular Physiology, Yale School of Medicine [New Haven, Connecticut] (YSM), University of Basel (Unibas), Hans Berger Klinik für Neurologie, Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], Institute of Neuroscience and Center for Integrated Protein Science, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), School of Medicine, University of Pennsylvania, Department of Neurobiology, Weizmann Institute of Science [Rehovot, Israël], Department of Neuroscience, UConn Health Center, Farmington, Canepari, Zecevic, Yale University School of Medicine, and University of Pennsylvania [Philadelphia]
- Subjects
Time Factors ,Dendritic spine ,Light ,Dendritic Spines ,Nanotechnology ,Biology ,Article ,Membrane Potentials ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Biological neural network ,Animals ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Axon ,Fluorescent Dyes ,030304 developmental biology ,Action potential initiation ,Membrane potential ,0303 health sciences ,Dendritic spike ,Lasers ,Axons ,Voltage-Sensitive Dye Imaging ,Bivalvia ,medicine.anatomical_structure ,Synapses ,Nerve cells ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Neuron ,Nerve Net ,Single-Cell Analysis ,Neuroscience ,030217 neurology & neurosurgery - Abstract
A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on how exactly are signals integrated by individual nerve cells functioning as complex operational units. Regional electrical properties of branching neuronal processes which determine the input-output function of any neuron are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor, at multiple sites, subthreshold events as they travel from the sites of origin (synaptic contacts on distal dendrites) and summate at particular locations to influence action potential initiation. It became possible recently to carry out this type of measurement using high-resolution multisite recording of membrane potential changes with intracellular voltage-sensitive dyes. This chapter reviews the development and foundation of the method of voltage-sensitive dye recording from individual neurons. Presently, this approach allows monitoring membrane potential transients from all parts of the dendritic tree as well as from axon collaterals and individual dendritic spines.
- Published
- 2011