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Molecular case studies (MCSs) are open educational resources that use a storytelling approach to engage students in biomolecular structure-function explorations, at the interface of biology and chemistry. Although MCSs are developed for a particular target audience with specific learning goals, they are suitable for implementation in multiple disciplinary course contexts. Detailed teaching notes included in the case study help instructors plan and prepare for their implementation in diverse contexts. A newly developed MCS was simultaneously implemented in a biochemistry and a molecular parasitology course at two different institutions. Instructors participating in this cross-institutional and multidisciplinary implementation collaboratively identified the need for quick and effective ways to bridge the gap between the MCS authors' vision and the implementing instructor's interpretation of the case-related molecular structure-function discussions. Augmented reality (AR) is an interactive and engaging experience that has been used effectively in teaching molecular sciences. Its accessibility and ease-of-use with smart devices (e.g., phones and tablets) make it an attractive option for expediting and improving both instructor preparation and classroom implementation of MCSs. In this work, we report the incorporation of ready-to-use AR objects as checkpoints in the MCS. Interacting with these AR objects facilitated instructor preparation, reduced students' cognitive load, and provided clear expectations for their learning. Based on our classroom observations, we propose that the incorporation of AR in MCSs can facilitate its successful implementation, improve the classroom experience for educators and students, and make MCSs more broadly accessible in diverse curricular settings.

This article aims to simplify and facilitate the process of practical teaching of enzyme kinetics by utilizing minimal teaching laboratory requirements. Simultaneously, it ensures that students comprehend the enzyme kinetics experiment effectively. The focus is on teaching students how to estimate the maximum velocity (Vmax) and Michaelis constant (Km) of [beta]-fructofuranosidase enzyme (also known as invertase) isolated from dry yeast. The invertase enzyme catalyzes the hydrolysis of sucrose substrate into glucose and fructose, employing the Michaelis-Menten approach of evaluating invertase enzyme kinetics as well as Lineweaver-Burk linear graphic approach of evaluating the Michaelis-Menten enzyme kinetics. The practical experiment seeks to reinforce the concepts of initial velocity dependence on substrate concentration. The data presented in the work were generated from a genuine practical biochemistry course enrolled by second-year undergraduate students in the Department of Pharmacy and the Department of Medical Laboratory Science. While there were minor variations in the invertase enzyme kinetic parameters among students, they successfully carried out the experiment. The students accurately estimated the Vmax and Km of the invertase enzyme in the sucrose hydrolysis chemical reaction. Moreover, they demonstrated an understanding of the meanings of the kinetic parameters (Km and Vmax) and the utility of the Lineweaver-Burk plot.

The present study has aimed to develop and validate a protein concept inventory (PCI) consisting of 25 multiple-choice (MC) questions to assess students' understanding of protein, which is a fundamental concept across different biology disciplines. The development process of the PCI involved a literature review to identify protein-related content, validation interviews to iteratively validate and refine the created items (n = 26), and data collection from a large sample (n = 291) for statistical analysis. An expert interview was held with two different field experts regarding the content validity of the draft PCI tool, the suitability of the options, and the clarity of the items. Free choice format (multiple marking) was used to answer the developed MC items. In scoring these items, positive points were given to correct options, and negative points were given to incorrect options. Evidence regarding the psychometric properties of the PCI trial form was collected through factor analysis, group differentiation, internal consistency, and item analysis using quantitative data. The evidence collected demonstrates that the validity and reliability of the PCI as a measurement tool have been confirmed. PCI's scoring approach and the use of response patterns created by multiple markings in teaching are discussed.

Plant metabolite compounds have been applied on plant cellular metabolism, produces organic and inorganic compounds, primary and secondary bioactive molecules, such as glucose, amino acids, fat acids, alkaloids, flavonoids, and terpenoids. Undergraduate biology students learn about the plant terpenoid assisted by bioinformatics to store, manage, and interpret the molecular information about these compounds. This research aims to observe the students' understanding of terpenoid through implementing bioinformatics approach. Besides, to investigate how practice the bioinformatics technology in learning terpenoid may impact students' comprehension of plant metabolism domain and their acquisition of bioinformatics inquiry skills. Bioinformatics is a computational database that relies on digital repositories of molecular biology informations. Data analysis was in the form of quantitative and qualitative descriptive using module learning resources based on terpenoid research and assisted by bioinformatics. The results indicate that students' comprehension of learning and understanding terpenoid has improved, identification and analysis processes of article reviews showed that students were able to discuss and interpret research finding in silico bioinformatics using molecular docking procedures. Assessment of bioinformatics skills showed that all undergraduate biology students could follow the direct instructions well, answer the questions, practice the dry-lab experimental, and formulate the conclusion correctly.

Learning media is a tool that makes it easier for educators to convey material to students effectively and efficiently to improve the quality of learning. Biology subjects in the essential enzymes and cell metabolism competencies require precision and accuracy, so appropriate learning media are needed. Booklets are a medium that can make it easier for students to learn. They are small, attractive, practical, simple, and easy to carry anywhere. This research aims to determine the suitability of booklet media for enzyme material and cell metabolism based on the acute toxicity test of Zuriat seed extract. This research's stages are determining potential problems, collecting information, designing booklets, validating booklets, revising booklets, and making booklets. The initial stage of making the booklet is to carry out an acute toxicity test of Zuriat seed extract and design the contents of the booklet. The suitability of the booklet is seen from the assessment carried out by five validators. The research instrument uses a validation sheet. The aspects assessed include format, content, and language. The research results show that the booklet can be declared suitable as a learning medium. The value for the format, content, and language aspects is 1. The results of the validation show the CVI value is 1. It can be concluded that the class XII booklet on enzymes and cell metabolism is based on the acute toxicity test Zuriat seed extract as a learning medium, which can be declared feasible.

This study was done to find out what students thought about the variables of perceived control, perceived learning, perceived benefit, interest, motivation, affective components, attitude, behavioral components, and learning experience when using digital games in information and communication technologies courses. As a case study, the study was created. 30 students from the biochemistry department made up the study's research group. The tool for gathering data was a semi-structured interview form. Data analysis was done using the content analysis method. When the study's results were discussed, students claimed that playing digital games improved their perceptions of their level of control, interest in the subject, motivation, perceived value of the course, learning experiences, and learning facilitation. Students also mentioned that they felt a variety of positive emotions while using digital games, that they wanted to play them constantly and willingly, and that their attitudes had improved. To increase and enhance students' interest in the course, motivation, positive attitudes and emotions, desire, and learning experiences, digital games can be used in technology-focused courses.

Background: ADHD is highly comorbid with Delayed Sleep Phase Syndrome (DSPS). Both are associated with obesity and diabetes, which can be caused by long-term dysregulations of appetite and glucose metabolism. This study explores hormones involved in these processes and the effects of chronotherapeutic interventions in a small sample of adults with ADHD and DSPS. Methods: Exploratory, secondary analysis of data from the PhASE study, a three-armed randomized clinical trial, are presented, including 37 adults (18-53 years) with ADHD and DSPS receiving three weeks of 0.5 mg/day (1) placebo, (2) melatonin, or (3) melatonin plus 30 minutes of bright light therapy (BLT). Leptin (appetite-suppressing), ghrelin (appetite-stimulating), insulin, insulin-like growth factor-1 (IGF-1), and glucose were measured from blood collected at 08:00 hours. Salivary cortisol was collected during the first 30 minutes after awakening and self-reported appetite was assessed. Results: Baseline leptin and IGF-1 levels were higher than reference ranges, and ghrelin and cortisol levels were lower, while insulin and glucose were normal. Melatonin treatment decreased leptin and insulin. Other outcomes remained unchanged and melatonin + BLT had no effects. Conclusion: Due to the small sample size and exploratory nature of the study, results should be interpreted with caution. Overall, these results show no strong indications for dysregulation of appetite and glucose metabolism to suggest high risk of obesity and diabetes in this small sample of adults with ADHD and DSPS. However, baseline appetite was suppressed, likely because measurements took place in the early morning which could be considered the biological night for this study population. Melatonin treatment seemed to cause subtle changes in appetite-regulating hormones suggesting increased appetite. Chronotherapeutic treatment may affect appetite-regulating hormones by advancing the biological rhythm and/or altering eating behaviors, but this remains to be investigated in larger samples using detailed food diaries.

Intra-individual response time variability (IIRTV) during cognitive performance is increasingly recognized as an important indicator of attentional control (AC) and related brain region function. However, what determinants contribute to preschoolers' IIRTV received little attention. The present study explored the interaction of dopaminergic polygenic composite score (DPCS) and the parent-child relationship in relation to preschoolers' IIRTV. In the initial sample, 452 preschoolers (M age = 5.17, SD = 0.92) participated in the study. The modified Flanker task was used to evaluate children's IIRTV and their parents were requested to complete the Parent-Child Relationship Scale to assess the parent-child relationship (closeness/conflict). DNA data were extracted from children's saliva samples, and a DPCS was created by the number of "COMT," "DAT1," and "DRD2" alleles associated with lower dopamine levels. Results showed that DPCS significantly interacted with the parent-child closeness to impact preschoolers' IIRTV. Specifically, preschoolers with higher DPCS exhibited lower IIRTV under higher levels of the parent-child closeness, and greater IIRTV under lower levels of the parent-child closeness compared to those with lower DPCS, which supported the differential susceptibility theory (DST). A direct replication attempt with 280 preschoolers (M age = 4.80, SD = 0.86) was conducted to investigate whether the results were in accordance with our exploratory outcomes. The interactive effect of DPCS and the parent--child closeness on IIRTV was confirmed. Additionally, the significant interactive effect of DPCS and the parent-child conflict on IIRTV was found in the replication study. The findings indicate that preschoolers' IIRTV, as an indicator of AC and related brain region function, is influenced by the interactions of dopaminergic genotypes and the parent-child relationship.

Given the severe pollution caused by traditional plastics, biodegradable plastic products (BPPs) have received great attention and are appearing in people's lives in the form of packaging bags, tableware, etc. Whether a wide variety of BPPs can truly degrade depends on the environment. Aerobic composting is the arbitration method to evaluate the degradability of BPPs in a premarket review, so only the aerobic degradability of BPPs can be guaranteed. Anaerobic digestion has become an irreplaceable part of the global carbon cycle and a mainstream technique to dispose of organic wastes. However, little attention has been given to the anaerobic degradation of BPPs, which may cause environmental pollution if BPPs are not degraded within a short time period. Students are not aware of these issues. Therefore, the teacher carried out teaching reforms in biochemistry course by designing anaerobic degradation of commercially available BPPs as a laboratory exercise for undergraduate students to form a correct understanding of the actual anaerobic degradation performance of BPPs, establish a more comprehensive theoretical knowledge framework of catabolism in biochemistry course, and train their hands-on ability and operation skills. This paper provides an introduction to the experimental design and teaching process of the anaerobic degradation of BPPs, and the results show that the degradation degree and rate are closely related to the components of BPPs. The results of the quizzes and questionnaires show that teaching reform is not only beneficial for deepening students' impression and understanding of anaerobic catabolism from ubiquitous BPPs in everyday life but also helpful for promoting their environmental awareness by selecting and using BPPs with better degradability. This teaching reform offers references and guidance for the teaching practice in courses like biochemistry, environmental microbiology, environmental chemistry, and waste treatment.

It has been shown that active learning strategies are effective in teaching complex STEM concepts. In this study, we developed and implemented a laboratory experiment for teaching the concepts of Boolean logic gates, molecular beacon probes, molecular computing, DNA logic gates, microRNA, and molecular diagnosis of hepatocellular carcinoma, which are related to DNA molecular computing, an interdisciplinary cutting-edge research technology in biochemistry, synthetic biology, computer science, and medicine. The laboratory experience takes about110-140 min and consists of a multiple-choice pretest (15 min), introductory lecture (20 min), wet laboratory experiment (60-90min), and a post-test (15 min). Students are tasked to experimentally construct three molecular logic circuits made of DNA oligonucleotides and use them for the fluorescence-based detection of microRNA markers related to diagnostics of hepatocellular carcinoma. The class was taught to undergraduate students from freshman to senior academic levels majoring in chemistry, biochemistry, biotechnology, and biomedical sciences. Students were engaged during the session and motivated to learn more about the research technology. A comparison of students' scores on the pretest and post-test demonstrated improvement in knowledge of the concepts taught. Visual observation of the fluorescence readout led to a straightforward interpretation of the results. The laboratory experiment is portable; it uses inexpensive nontoxic reagents and thus can be employed outside a laboratory room for outreach and science popularization purposes.

Purpose: To investigate serum levels of VEGF, IGF-1 and FGF-2, and relationships with several clinical characteristics in children and adolescents with autism spectrum disorder (ASD) with and without bipolar disorder (BD). Method: 40 subjects with ASD + BD as study group, and 40 subjects with ASD as control group were included. Serum levels of VEGF, IGF-1, and FGF-2 were measured using commercial enzyme-linked immunosorbent assay kits. Results: The study group was significantly higher than the control group in terms of ASD severity, self-harming behavior and sleep disturbance. Serum VEGF and FGF-2 levels were significantly higher in the ASD + BD group than in the control group. There was no significant difference in serum IGF-1 levels between the two groups. There was no correlation between VEGF, IGF-1 and FGF-2 serum levels and ASD severity in the study group. However there was a negative correlation between VEGF levels and age at first diagnosis of BD, and a positive correlation between IGF-1 levels and the number of bipolar episodes in the study group. Conclusion: Growth factors like VEGF and FGF-2 may be potential biomarkers of bipolar disorder in young subjects with ASD. Given the difficulty of clinical management of BD in young subjects with ASD, potential biomarkers would help clinicians in the diagnosis and follow up of BD in this special population. Further research is needed whether VEGF and FGF-2 can be potential biomarkers in the clinical management of young subjects with ASD and BD.

Team-based learning (TBL) is a highly intense active learning pedagogy that uses a cycle of preclass preparation, formative assessment for readiness, mini-lectures, and complex team exercises. Consistent with the literature on active learning, prior research on TBL consistently shows its benefits for student outcomes as compared with previous lecture. However, little work has examined student outcomes disaggregated by demographic variable. We analyzed assessment and student demographic data (i.e., binary gender, racial/ethnic group, generational status) in three semesters of TBL in an upper-division biochemistry course to understand the degree to which performance could be predicted by student demographics. We use theoretical and empirical research from social psychology to hypothesize that the intense interpersonal interactions of TBL could activate psychological threats, the effects of which would be measurable as differences in student performance that correlate with demographic variables. Our regression analysis did not support this hypothesis. This null result invites a deeper discussion on how we measure the potential effects of active learning on student outcomes, particularly given how important it is to account for intersectional and invisible identities.

The central dogma of molecular biology describes the transfer of genetic information from nucleic acids to proteins and stipulates that the system cannot work in the reverse direction. As a fundamental principle in biology, the dogma is as influential as it is controversial. Some commentators have debated the central dogma's empirical accuracy because they believe that some exceptions are incompatible with the central dogma. We investigated these exceptions challenging the central dogma and conclude that they do not violate the central dogma. The central dogma is still a common model used to describe and study the relationship between genes and proteins. This is one of the greatest established achievements in modern biology.

Are children from "Eastern" cultures less emotionally expressive and reactive than children from "Western" cultures? To answer this, we used a multi-level and multi-contextual approach to understand variations in emotion displays and cortisol reactivity among preschoolers living in China and the United States. One hundred two preschoolers from China (N = 58; 55% males) and the United States (N = 44, 48% males) completed three (i.e., control, interpersonal-related, and achievement-related) emotion-challenging paradigms over 3 days. Behavioral emotion expressions were coded, and salivary cortisol was sampled 30 minutes before and across 90 minutes post-task. Without considering context, Chinese preschoolers displayed fewer levels of positive and negative emotion expressions relative to their United States counterparts. However, Chinese preschoolers displayed similar levels of expressions as their United States counterparts during an achievement-related challenge that is more salient to their sociocultural emphases and showed higher negative emotion expressions in this challenge, relative to other contexts. Moreover, only the achievement-related challenge elicited increased cortisol levels among Chinese preschoolers, and this was correlated with higher levels of negative expressions. For US preschoolers, no cortisol increase was observed in any challenging paradigms, nor was cortisol associated with emotional expressions. Findings counter prior notions that East Asian children are generally less emotionally expressive. Instead, an achievement-related challenge elicited higher emotion expression and cortisol reactivity among Chinese preschoolers, suggesting that children's emotion expression and biological reactivity may be most responsive to contexts salient to their socio-cultural environments. We discuss the importance of considering cultural contexts when studying emotion regulation.

Background: Lipid metabolism plays an essential role in nervous system development. Cholesterol deficiency leads to a variety of neurodevelopmental disorders, such as autism spectrum disorder and fragile X syndrome. There have been a lot of efforts to search for biological markers associated with and causal to ADHD, among which lipid is one possible etiological factor that is quite widely studied. We aimed to evaluate the causal relationship between lipids traits, lipid-lowering drugs, and attention deficit hyperactivity disorder (ADHD) outcomes using Mendelian randomization (MR) studies. Methods: We used summary data from genome-wide association studies to explore the causal relationships between circulating lipid related traits and ADHD. Then, quantitative trait loci for the expression of lipid-lowering drug target genes and genetic variants associated with lipid traits were extracted. Summary-data-based MR and inverse-variance-weighted MR (IVWMR) were used to investigate the correlation between the expression of these drug-target genes and ADHD. Results: After rigorous screening, 939 instrumental variables were finally included for univariable mendelian randomization analysis. However, there is no correlation between lipid profile and ADHD risk. Drug target analysis by IVW-MR method observed that APOB-mediated low-density lipoprotein cholesterol was associated with lower ADHD risk (odds ratio [OR] = 0.90, 95% confidence interval [CI] [0.84, 0.97]; p = .007), whereas "LPL"-mediated triglycerides levels were associated with a higher risk of ADHD (OR = 1.13, 95% CI [1.06, 1.21]; p < .001). Conclusion: Our results suggest that "APOB" gene and "LPL" gene may be candidate drug target genes for the treatment of ADHD.

Gamification has a strong track record of improving student engagement and learning in the chemical sciences. Meta-analyses of different approaches to gamification have highlighted that providing a game fiction, encouraging students to work in teams, and breaking games into smaller "quests" are particularly effective. Here, we aimed to increase students' engagement and learning with four analytical biochemistry techniques (high-performance liquid chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blot, and mass spectrometry) by creating a game that required them to use every method to solve a "murder mystery". Our game was based on the popular games "Mafia" and "Among Us" and introduced an original game fiction relevant to our setting. Students attending the formative gamified session gave highly positive feedback. They indicated that they enjoyed the session and increased confidence in the methods involved. Free text comments praised many of the elements deliberately introduced into the game. To our surprise, the main criticism was that the session was not challenging enough. We developed a revised session that required students to undertake more detailed data interpretation, which students reported gave the expected increase in difficulty. All materials and code for running our session and generating new mysteries with original data are available online.

Investigating and understanding novel antibacterial agents is a necessary task as there is a constant increase in the number of multidrug-resistant bacterial species. The use of nanotechnology to combat drug-resistant bacteria is an important research area. The laboratory experiment described herein demonstrates that changes in the nanostructure of a material lead to significantly different antibacterial efficacies. Silver has been known to be an effective antibacterial agent throughout history, but its therapeutic uses are limited when present as either the bulk material or cations in solution. Silver nanoparticles (AgNPs)and DNA-templated silver nanoclusters (DNA-AgNCs) are both nanostructured silver materials that show vastly different antibacterial activities when incubated with "E. coli" in liquid culture. This work aims to provide students with hands-on experience in the synthesis and characterization of nanomaterials and basic microbiology skills; moreover, it is applicable to undergraduate and graduate curricula.

Industrial drug discovery teams encompass scientists from multiple specialties and require participants to communicate effectively across disciplinary boundaries. In this paper, we present an undergraduate or graduate classroom simulation of this environment. Over a series of five workshops, student teams of mixed scientific backgrounds perform five iterations of the chemistry cycle of small molecule drug discovery. Students analyze physicochemical, structural, and (fictional) assay data and use these to design new compounds for testing. Simulated assay results are returned to students who use the information in the design of subsequent compounds. After workshop 5, each team selects a single lead compound, supported by a potential synthetic route, a portfolio of assay data, and logical scientific decision-making. Our exercise provides students with opportunities for hands-on student-responsive data handing, team-building, and technical knowledge acquisition--all within an industrially relevant scientific scenario.

Access to high-quality early music education programs may mitigate the effects of poverty on the hypothalamic-pituitary-adrenal (HPA) axis, but fundamental questions remain about the role of early educators in conveying these benefits. In the current study, we measured the basal or resting cortisol levels of 76 children (M[subscript age] = 4.17 years; 42% female) over the course of the school day while they attended a Head Start preschool that included early music education classes. The results of a series of hierarchical linear models (HLMs) indicated that child-directed music and movement activities during these classes were associated with lower levels of cortisol (relative to teacher-directed activities; B = -0.019, p = 0.013), as were higher quality teacher-child interactions (B = -0.018, p = 0.013); both associations were moderated by child age. We discuss the implications of these results for future educational neuroscience research that seeks to inform early education programs for young children placed at risk by poverty.

Rheumatic diseases are known to be associated with the development of metabolic syndrome, which increases mortality rates due to cardiovascular complications. Although a variety of self-management programs for rheumatic diseases have been developed, few have concentrated on metabolic syndrome. This study aimed to develop and verify a blended (a mixture of telephone and online interventions) metabolic syndrome self-management program. The program was developed in four stages: analysis to identify program contents, website design, website development, and validity testing. A quasi-experimental nonequivalent control group, pretest-posttest design was adopted to verify the program effectiveness in 54 patients with rheumatic disease. The program was initially implemented via telephone for 4 weeks and then self-administered via a web-based platform for 20 weeks. Indices of metabolic syndrome and self-management and quality of life scores were measured as outcome variables. Data were collected three times: before intervention, after 4 weeks of telephone interventions, and after 20 weeks of online self-interventions. The devised program had a significant overall effect on metabolic syndrome indices, metabolic syndrome-related self-management behaviors, and quality of life. Detailed analysis showed the program effectively reduced body mass index, waist circumference, systolic blood pressure, and blood glucose levels and improved metabolic syndrome self-management behaviors. Based on our findings, it can be expected that the use of this program may retard or prevent the progression of metabolic syndrome by improving some metabolic syndrome indices and metabolic syndrome-related self-management behaviors, which are key components of care in rheumatic disease patients with metabolic syndrome. This web-based program appears to be beneficial in public health care settings because it is cost-effective, readily available, and may provide long-term support.

Biology is perhaps the most complex of the sciences, given the incredible variety of chemical species that are interconnected in spatial and temporal pathways that are daunting to understand. Their interconnections lead to emergent properties such as memory, consciousness, and recognition of self and non-self. To understand how these interconnected reactions lead to cellular life characterized by activation, inhibition, regulation, homeostasis, and adaptation, computational analyses and simulations are essential, a fact recognized by the biological communities. At the same time, students struggle to understand and apply binding and kinetic analyses for the simplest reactions such as the irreversible first-order conversion of a single reactant to a product. This likely results from cognitive difficulties in combining structural, chemical, mathematical, and textual descriptions of binding and catalytic reactions. To help students better understand dynamic reactions and their analyses, we have introduced two kinds of interactive graphs and simulations into the online educational resource, Fundamentals of Biochemistry, a LibreText biochemistry book. One is available for simple binding and kinetic reactions. The other displays progress curves (concentrations vs. time) for simple reactions and complex metabolic and signal transduction pathways. Users can move sliders to change dissociation and kinetic constants as well as initial concentrations and see instantaneous changes in the graphs. They can also export data into a spreadsheet for further processing, such as producing derivative Lineweaver-Burk and traditional Michaelis-Menten graphs of initial velocity (v[subscript 0]) versus substrate concentration.

Designing a relevant and engaging curriculum for biochemistry undergraduates can be challenging for topics which are at the periphery of the subject. We have used the framework of context-based learning as a means of assessing understanding of quantum theory in a group of students in their junior year. Our context, the role of retinol in skincare, provides a basis for the simple application of quantum mechanical principles to a biological context in an adaptation of the polyene in a box concept. As part of the learner journey, they gain experience of practical computational chemistry, which provided an in silico alternative to traditional laboratory work during the SARS-CoV-19 pandemic. Student feedback was overwhelmingly positive, and this approach is now firmly embedded in the undergraduate curriculum.

This article presents a study on the implementation of a virtual escape-room game as a novel teaching methodology in biochemistry education. The game aimed to engage students in producing monoclonal antibodies against SARS-CoV-2 while reinforcing theoretical concepts and fostering teamwork. Three versions of the game were tested, incorporating modifications to address student feedback on and improve the overall experience. The study employed a satisfaction survey to gather insights from students regarding their perception of the game. Results showed that the implementation of answer flexibility using RegEx had a significant positive impact on student satisfaction and motivation. The introduction of RegEx allowed for a more realistic and immersive gaming experience, as students could provide varied answers while still being evaluated correctly. Overall, the findings highlight the effectiveness of the game's design, the suitability of the Google Forms platform for distance learning, and the importance of incorporating answer flexibility through RegEx. These results provide valuable guidance for educators seeking to enhance student engagement and satisfaction through the use of escape-room games in biochemistry education.

Alcohol consumption has profound effects on behavior, such as impaired judgment, addiction or even death. It is estimated that alcohol contributes to around three million deaths worldwide, 13.5% of them in young people with ages between 20 and 39 years. Consequently, it is necessary to raise awareness among college and high school students of the risk related to alcohol drinking. The small nematode "Caenorhabditis elegans" is an animal widely used as a model organism to study nearly all aspects of Biochemistry. It is a powerful tool to test the potential bioactivity and molecular mechanisms of natural compounds and drugs "in vivo." Therefore, it is an interesting topic to include in an undergraduate course of Biotechnology, Biochemistry or Biology students among other scientific vocations. "C. elegans" is also used as a neurobiological model to evaluate substances' neurotoxicity and behavioral effects. The proposed experiment introduces students to the handling of this preclinical model and to the evaluation of behavioral alterations induced by chemicals in scientific research. The effects of different doses of ethanol on "C. elegans" behavior are studied using a versatile chemotaxis assay. This laboratory experiment is suitable for an undergraduate course. The practical session can be used in the global strategies of information and awareness of educational centres to mitigate the impact of alcohol abuse among students, both in formal courses or in Science fairs or exhibitions.

Meta-analysis is a useful tool in clinical research, as it combines the results of multiple clinical studies to improve precision when answering a particular scientific question. While there has been a substantial increase in publications using meta-analysis in various clinical research topics, the number of published meta-analyses in metabolomics is significantly lower compared to other omics disciplines. Metabolomics is the study of small chemical compounds in living organisms, which provides important insights into an organism's phenotype. However, the wide variety of compounds and the different experimental methods used in metabolomics make it challenging to perform a thorough meta-analysis. Additionally, there is a lack of consensus on reporting statistical estimates, and the high number of compound naming synonyms further complicates the process. Easy-Amanida is a new tool that combines two R packages, "amanida" and "webchem", to enable meta-analysis of aggregate statistical data, like p-value and fold-change, while ensuring the compounds naming harmonization. The Easy-Amanida app is implemented in Shiny, an R package add-on for interactive web apps, and provides a workflow to optimize the naming combination. This article describes all the steps to perform the meta-analysis using Easy-Amanida, including an illustrative example for interpreting the results. The use of aggregate statistics metrics extends the use of Easy-Amanida beyond the metabolomics field.

Outreach science labs aim to promote students' interest. Previous research has often suggested that performing experiments in such labs has a positive effect on their interest. However, these studies often lack a comparison to the effects of performing them at school. This research gap was addressed in the present study. The sample consisted of 402 upper-secondary level students (age: M = 16.53 years, SD = 0.80 years) who performed three experiments on the topic of enzymology either in an outreach science lab (n = 203) or at school (n = 199). Contrary to the assumption, experimentation at the outreach science lab did not outperform experimentation at school in terms of students' psychological state of interest in the comparison to the school setting. Surprisingly, differences in the value-related component of the psychological state of interest were even found in favor of the school treatment.

Diabetes mellitus and obesity are major public health issues that significantly impact the health care system. The next generation of health care providers will need a deep understanding of the pathophysiology of these diseases if we are to prevent, treat, and eventually cure these diseases and ease the burden on patients and the health care system. Physiology core concepts are a set of core principles, or "big ideas," identified by physiology educators that are thought to promote long-term retention, create a deeper understanding, and help with formation of critical thinking skills. Here we describe our scaffolded teaching approach in an upper year undergraduate pathophysiology course to educate students about these two diseases and discuss how learning about the basis of these highly integrative diseases from the biochemical to whole body level is a meaningful tool in the physiology educator toolbox to reinforce physiology core concepts. This teaching strategy is designed to engage students in the scientific process and hone their problem-solving skills such that they are hopefully equipped to treat and eventually cure these diseases as they move forward in their careers.

Competency-based physiology and biochemistry education can benefit from the creative integration of imaginative narratives into traditional teaching methods. This paper proposes an innovative model using a pen and palm analogy to visualize enzyme function theories. The pen (substrate) must fit snugly into the palm (enzyme's active site) for catalysis to occur, akin to induced-fit theory. Pressing the pen's top button with the thumb represents the strain needed to convert substrate (pen with nib inside) into product (pen with nub out, ready to write). By leveraging everyday objects creatively, students can enhance their understanding and engagement with enzymatic reactions.

Sanger sequencing, also known as dideoxy sequencing, is a widely used method for DNA sequencing, particularly for cloned plasmids and clinical samples. This technique requires a combination of essential biochemistry skills, such as a chain-termination reaction, gel electrophoresis, and fluorescence detection. Unfortunately, there is a lack of activities that replicate the Sanger sequencing process for students to learn and practice these skills. To address this issue, a manipulative-based Sanger sequencing activity was developed that incorporates colorful pop beads to demonstrate a chain-termination reaction, separation of products, and fluorescence detection. The beads represent deoxynucleotides and dideoxynucleotides, allowing for a visual representation of DNA fragment generation. This kinesthetic learning activity offers a high visual impact for students, aiding in their understanding of the Sanger sequencing process, and can also be used to illustrate polymerase chain reaction (PCR)-based techniques.

The three-dimensional nature of macromolecules is often difficult for undergraduate students to grasp. This leads to difficulties in understanding key concepts in Biochemistry, such as protein function and conformational change. Virtual reality (VR) technologies, which can aid students in three-dimensional visualizations, have been shown to increase student motivation, but published reports do not universally agree about whether VR improves student comprehension. Here we present the implementation of a VR experience that was designed to complement existing biochemistry experiments and an analysis of both student engagement with and understanding of the material presented in VR. Results indicate that students enjoyed this interactive, immersive activity and suggest evidence of increased understanding. However, the effectiveness of the VR experience--and effective assessment of such an experience--may depend on a number of factors.

Electrophoresis is integral to analytical and biochemistry experiences in undergraduate education; however, fundamental principles of the method are often taught in upper-level laboratories through hands-on experiences. A laboratory activity is reported that teaches the concepts of electrophoretic mobility and electroosmotic flow. A single reuseable instrument, called a mini-E, costs 37 USD and consists of a DC power supply, a voltmeter, platinum electrodes, and a chip cast in polydimethylsiloxane. This activity uses common reagents costing only 0.02 USD per student. Experiments are devised that allow students to investigate the properties of electrophoretic flow and electroosmotic flow by separating the two commonly used food dyeing agents Brilliant Blue FCF and Allura Red AC in vinegar and in a solution of ammonium hydroxide. A dark-purple mixture of these dyes is separated into red and blue bands that are easily visualized. The migration order of the dyes differs when the separation is performed under conditions of reversed polarity and suppressed electroosmotic flow (vinegar) compared to conditions of normal polarity and active electroosmotic flow (ammonium hydroxide). When delivered to chemistry majors, students had a significant gain in their ability to apply the concepts of electroosmosis and electrophoresis to predict analyte migration. Although this activity targets upper-level chemistry content, it can also be adapted for other laboratory experiences.

The high level of consumption of processed foods has an effect on human health. The consumption of doughnuts, pastries, fried chicken, French fried potatoes, snack chips, and imitation cheese is high among teenagers. These foods are generally rich in fats, although the focus has often been on the quantity rather than the quality of these fats. These products often contain saturated and trans fatty acids, which have adverse effects on triglycerides and cholesterol levels and raise lipoprotein(a) levels in plasma. These modifications are associated with the risk of developing certain health conditions, such as heart disease, stroke, type 2 diabetes, and obesity. It is important to note that not all fats have this negative effect on health. Dietary fats are an essential part that cannot be substituted for a healthy diet. A diet rich in healthy fats, such as monounsaturated and polyunsaturated fats, has an important benefit in human health. For this reason, it is crucial to read, identify, and interpret food labels in processed food to identify the presence of saturated or trans fats and choose healthier alternatives. Therefore, in this workshop, students in the first year of baccalaureate extracted oils and fats from a range of processed foods, quantifying and characterizing their nature by analyzing their fatty acid composition by gas chromatography. Subsequently, students compared their results according to the information given on the food package label and the literature. Finally, the students gave a talk on the work done in the laboratory and the knowledge gained from the students in lower grades, third and fourth years of high school, to raise awareness among these students.

Retrospective alcohol use data are prone to recall bias, a limitation that could be addressed with real-time ecological momentary assessment (EMA) tools. We aimed to (1) introduce a simple (single-click) EMA methodology for collecting real-time alcohol use data, and (2) investigate the EMA methodology's performance relative to established alcohol use data collection tools. In March-April 2021, we sampled undergraduate students (n = 84) and collected a week of alcohol use data. Participants entered their real-time drinking start times using our EMA methodology, self-reported their drinking details in daily surveys, and a subsample recorded their breath alcohol concentration (BrAC) using smart breathalyzers. We estimated the accuracy of our EMA methodology in collecting alcohol use data relative to data collected by daily surveys and breathalyzers. Overall, 199 drinking events were recorded with the EMA methodology. Numbers of drinks recorded with the EMA methodology were correlated with self-reported daily surveys (r = 0.82, p < 0.001) and BrAC readings (r = 0.69, p < 0.001). Sensitivity and specificity of the EMA methodology in detecting heavy drinking relative to daily surveys were 82% (95% CI [67%, 92%]) and 97% (95% CI [85%, 100%]), respectively. These were 74% (95% CI: [64%, 83%]) and 92% (95% CI: [85%, 96%]) for binge drinking. Similar results were found when we used breathalyzers as the reference standard test. We developed an EMA methodology for collecting real-time alcohol use data (alcohol drinking start-time, frequency, magnitude, patterns, and pace). Our findings support the utility of our EMA methodology in collecting alcohol use data among college students.

The authors, a chemistry professor and a librarian, used a qualitative survey to assess student perceptions of a Wikipedia editing assignment that they included in a large upper-level biochemistry course. The assignment was initially intended as a public-facing alternative to a short research paper, emphasizing information literacy and scientific literacy. The goal of the survey was to use the results to enhance the assignment. The results of the survey and research for the literature review inspired a novel approach to the assignment using the perspective of metaliteracy. This approach encourages students to think critically about their role as scholars in a participatory environment.

The COVID-19 pandemic crisis has modified teaching and learning throughout the world. The developments in Information Communication Technology tools have played a critical role in the education community. During the pandemic, the organization and conduction of theory classes in Universities across India were managed reasonably on one side, but the problem existed in laboratory courses. A singlepoint portal is needed to organize and conduct a laboratory course in a virtual mode. While creating a virtual lab (based on the syllabus) would solve the need, creating such would be a tedious task for the teachers, requiring special skills and resources. Google Sites is a free website application tool that allows users to create and edit files online while collaborating with others in real-time. The application includes other Google applications such as Google Docs, Google Spreadsheets, Google Slides, Google Forms, Google Drawings, and Google Keep. Google Sites also accepts any online links or files, which is handy for creating a simplified virtual curation lab for free using common online resources.

Undergraduate courses such as biochemistry were prompted to embrace online distance learning triggered by the incursion of the COVID-19 pandemic. This study worked to help solve the issues and concerns related to teaching biochemistry as a foundational course for dental and other health-sciences programs in the status of the educational system. The case study method was deemed a good way to focus on the objectives of this research because the theme was dentistry students' perceptions and experiences dealing with the opportunities and difficulties that the COVID-19 pandemic brought with it in this circumstance. 11 purposively sampled dentistry students served as the participants of the study. Data were gathered through an online platform with open-ended questions which were thematically analyzed afterward. The identified opportunities were lumped into ROTE: Research works, Open and independent learning, Technology for education, and Education for health. Challenges encountered were grouped as DARE: Demand for technology, Acquisition of knowledge, Remote learning, and Environmental condition for studying. It is worth noting that the participants were enlightened on the academic and personal benefits of biochemistry online distance learning. The researchers appeal to future researchers and policymakers to anchor forthcoming steps to further augment the online teaching and learning experiences of teachers and learners.

Specifications grading is a relatively recent approach to assessing student learning. In this approach, students make progress toward completion of a course by demonstrating mastery of specific skills or material. The assessment tools are short, frequent exercises that can be attempted multiple times until mastered. This contrasts with the traditional, exam-based assessment of student learning. There are multiple benefits to the specifications grading-based strategy, including reduced test anxiety, better knowledge retention, and increased flexibility. In this study, specifications grading was implemented into an upper-level biochemistry course at a private, liberal arts university. The student cohort consisted almost exclusively of junior and senior biochemistry, biology, and chemistry majors. Students earned points for demonstrating mastery on each of 12 short quizzes in addition to points earned from laboratory exercises and on the cumulative final exam. Student attitudes were assessed using three surveys that were administered at the beginning, middle, and end of the course. The survey results indicated that the students had overall favorable opinions of the specifications grading approach and its use in this course. A comparison of student performance on the quizzes to their performance on the final exam showed that the students learned and retained the course material. Combining the survey and performance data, we demonstrated that the students' perceptions of their learning correlated well with their performances on the specifications grading tools. Together, these results indicated that specifications grading is an effective approach to assessing student learning and to maintaining student enthusiasm in an upper-level biochemistry course.

A renovation incorporated Innovative Learning Environments' design principles. The exploratory study used a Basic Mixed Method, Convergent Parallel Research Design. Behavioral observations and photographic tracing, two surveys (pre- and post-intervention), interviews, and instrument testing. Overall findings: (a) students and educators are excited to come to school, (b) far less negative/destructive behavioral issues, and (c) a sense of pride/belonging imbued. Specifically: (a) ILE designs at the ergo-dynamic furniture level are not as intuitive as hoped, (b) teaching practices guide the use of solutions, (c) students made intuitive use of the ergo-dynamic products in seated positions at both macro and micro levels, and (d) students still had too much sedentary time. Recommendation - provide holistic understanding for all stakeholders of ILEs' positive impact.

The aim of this current research was to investigate airborne fungi in indoor environments at primary schools. The study also examined the correlation between these fungi and building characteristics, as well as some geographical and meteorological parameters. To achieve this aim, a passive sampling method was used. In this study, 148 samples were collected from the indoor environments of 24 schools located in Qom, Iran. To collect the samples, passive sampling was performed using Petri plates containing Sabouraud dextrose agar (SDA). The characteristics of school buildings were evaluated by checklist. The mean ± SD fungal load of indoor air in selected schools was found to be 10.1 ± 14.0 colonies (CFU/dm2 / h). According to the IMA standard, the majority of the classes (71.7%) were in very good condition. The dominant species were as follows: "Aspergillus niger," "Aspergillus candidus," and "Aspergillus flavus." Furthermore, the fungal load of girls' schools was significantly higher than that of the boys' schools (p < 0.05). The correlation analysis using the Pearson test showed that there was a direct correlation between the mean fungal load of classrooms and the number of students (p < 0.01). The highest concentration of fungi was found on the ground floor and in poor ventilation conditions (p < 0.05). During the study, it was found that schools located in the western part of Qom, Iran, had a higher concentration of fungi. This can be attributed to their exposure to the prevailing winds and the penetration of outdoor dusty air into indoor environments. The large number of students in each class and the inappropriate ventilation, which are the causes of airborne culturable fungi of these classes, call for the need for proper operation of school buildings.

Creative thinking skills are recognised 21 century skills. This study aimed to determine whether students' creative thinking skills in biochemistry courses are improved through scaffolding-based problem-based learning. The study employed a mixed methods approach with a sample of 113 chemistry education programme students at Jambi University. An observation sheet instrument was used to gauge students' creative thinking skills during learning, test questions were used to gauge them after the implementation of Problem Based-learning, and an interview instrument was used to see how students responded once PBL learning had been implemented. Descriptive statistical testing techniques were applied to data between classes. It was found that there were significant differences in students' creative thinking skills after scaffolding-based PBL learning had been carried out. The biochemistry learning process was found to be conducive to scaffolding in problem-based learning. Providing scaffolding in problem-based learning also has a positive impact on students because this study proves that students become more active learners in education because the problem-solving process requires students to express opinions and exchange ideas.

Student ethnicity is one of the internal factors that influence student learning. Bacteriology is a course that examines prokaryotic unicellular living things and is practiced in laboratories that need to implement projects. The aim of this research is to know the distribution of students based on their ethnicity. The research sample was students who programmed the bacteriology course in the Biology Education Study Program at Mulawarman University, in the even semester of the 2022/2023 academic year. The sampling technique is purposive sampling. The research instrument is a questionnaire. The data analysis technique is percentage. The results of the data analysis show that there are various student ethnicities. There are four ethnic groups that dominate other ethnic groups, namely: Javanese (25.5%), Bugis (18.8%), Kutai (16.4%), and Banjar (14.9%). Completion of projects in groups whose members are heterogeneous based on ethnicity allows the participation of every member of the group. The conclusion is that there are various student ethnicities who program bacteriology courses, namely those consisting of ethnic Javanese, Bugis, Kutai, Banjar, Berau, Toraja, Paser, Mandar, Batak, Palembang, and Dayak. It is necessary to add a larger sample size for similar research in order to obtain more information.

Laboratory course activities often focus on "cookbook style," so their activities have not yet integrated with scientific thinking as an essential aspect. In this research, the laboratory experience of microbial analysis techniques was redesigned using alternative inquiry to involve students in isolating and characterising yeast to make the bread rise. This research aimed to determine the effect of implementing inquiry-based laboratory experiences on improving students' technical skills and the structure and characteristics of laboratory courses. The method used a single case study to obtain a complete picture regarding implementing laboratory course redesign. Laboratory experience activities consisted of pre-laboratory (reinforcement of theory) and investigation (preparation of methods, laboratory investigation activities, data analysis and presentation, and writing written reports). Overall, our results suggest that inquiry-based laboratory experiences can improve students' microbial analysis technique skills in strong collaboration. In addition, students considered several benefits from this learning, such as developing designs and conducting laboratory investigations close to the original context. With these results, educators can use the redesign of this laboratory course without applying full "course-based undergraduate research experiences" to students.

Biochemistry is becoming increasingly crucial for undergraduates majoring in biology, chemistry, agriculture, and medicine. In recent years, much research has been carried out on promoting this course's teaching outcomes. However, the knowledge structure of undergraduate biochemistry teaching research has yet to be formed. Here, 715 validated publications from 2012 to 2021 were retrieved from the Web of Science Core Collection. The annual publications, journals/cited journals, countries, institutions, authors/cited authors, keywords, and cited references were analyzed using CiteSpace. As revealed in our study, the United States is the most productive country, and "Biochemistry and Molecular Biology Education," "the Journal of Chemical Education," and "CBE Life Sciences Education" are the most influential journals. Most studies focused on introducing or designing creative laboratory courses to improve students' hands-on abilities. Interestingly, we found that the studies in this period can be divided into two stages: the research topics in the first stage were highly diverse, and in the second stage, the subfield of course-based undergraduate research experiences attracted much attention. Taken together, this study offers insights into the historical development of undergraduate biochemistry educational research, which will have implications for scientists conducting future investigations in this promising field and teachers for better learning outcomes.

Project-Enhanced learning is an excellent way to facilitate student-centered learning along with traditional lecture-based learning. In this Class and Home problem, an example of Project-Enhanced learning is provided that can be used in the Mass and Energy Balances (MEB) course. The students solve this problem as part of a group while receiving feedback from the professor and the teaching assistants. The overall learning objectives of this problem fulfill multiple ABET requirements.

Etiological beliefs of depression have differing impacts on motivation, hope, and treatment expectations. However, it is unclear where people are exposed to these beliefs. Objective: This study examined beliefs about depression and their relations to symptoms, attitudes about depression, and treatment preferences. Participants: 426 undergraduates attending a large midwestern university. Methods: Participants completed an online survey asking about causes of depression, if and where they had heard about the "chemical imbalance" explanation of depression, attitudes about depression, as well as measures of their symptoms, treatment history, and hypothetical treatment preferences. Results: Sixty-two percent of the sample had heard of the chemical imbalance explanation, most commonly from the classroom. Biochemical beliefs about depression were most strongly endorsed among participants with a family history of depression and who had had personal experience with treatment. The chemical imbalance belief was uniquely related to dysfunctional beliefs about depression. Etiological beliefs were largely unrelated to treatment preferences. Conclusion: College students are exposed to models of mental health that may not be ideal for treatment and recovery.

Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and cellular biology to pharmacology. However, because enzyme kinetics involves concepts rarely employed in other areas of biology, it can be challenging for students and researchers. Traditional graphical analysis was replaced by computational analysis, requiring another skill not core to many life sciences curricula. Computational analysis can be time-consuming and difficult in free software (e.g., R) or require costly software (e.g., GraphPad Prism). We present Enzyme Kinetics Analysis (EKA), a web-tool to augment teaching and learning and streamline EKA. EKA is an interactive and free tool for analyzing enzyme kinetic data and improving student learning through simulation, built using R and RStudio's ShinyApps. EKA provides kinetic models (Michaelis-Menten, Hill, simple reversible inhibition models, ternary-complex, and ping-pong) for users to fit experimental data, providing graphical results and statistics. Additionally, EKA enables users to input parameters and create data and graphs, to visualize changes to parameters (e.g., K[subscript M] or number of measurements). This function is designed for students learning kinetics but also for researchers to design experiments. EKA (enzyme-kinetics.shinyapps.io/enzkinet_webpage/) provides a simple, interactive interface for teachers, students, and researchers to explore enzyme kinetics. It gives researchers the ability to design experiments and analyze data without specific software requirements.