31 results on '"Besgen P"'
Search Results
2. Therapy-resistant dry itchy eyes
- Author
-
Rima Wardeh, Volker Besgen, and Walter Sekundo
- Subjects
Ophthalmology ,RE1-994 - Abstract
Abstract An 8 years old male presented to our clinic with dry eye symptomes. Different therapiy attemps were made in the last few months and did not lead to any improvement. Examining this patient revealed multiple signs of vitamin A deficiency, which could confirmed by laboratory examination. The initial substitution of vitamin A led to a fast rehabilitation and a following nutrition consulting kept the patient symptom-free over 6 month follow up. Vitamin A deficiency -although rare in the developed countries- is an importent differential diagnosis of the dry eye especially in children. Vitamin A deficiency not only causes ocular manifistaion, but also general symptoms. Dietary change and initial subtitution is the key element for a fast and sustaining improvement.
- Published
- 2019
- Full Text
- View/download PDF
3. 'Transductive Urbanism' A Method for the Analysis of the Relational Infrastructure of Malled Metropolitan Centres in Auckland, New Zealand
- Author
-
Manfredo Manfredini, Xin Tian, Ross Jenner, and Asu Besgen
- Subjects
Architecture ,NA1-9428 - Abstract
This article discusses the theoretical framework and methodology developed for research on comparative urbanism devoted to emerging phenomena in rapidly expanding cities. It focuses on problems related to the right to the city, particularly addressing questions of participation in the production of space. The exploration of progressive social and spatial fragmentation of urban environments concentrates on the peculiar changes occurring in the main nodes of Auckland, the largest polycentric urban area of New Zealand. Primary places of this urban transformation are the shopping malls of latest generation that dominate the newly formed metropolitan centres. The presented methodology aims to interpret the heterotopic spatial introversions of these centres and describe their specific forms of spatial transductions. Its specific analytical methods are designed to provide indications of the emerging transformation of the role of public space in the interpersonal sphere of sociability. This involves the exploration of the agency of new digital media as instruments for the emergence of independent and autonomous recombinant forms in the new urban condition. The methods are combined to analyse and compare conceptions and experiences of physical, social and eidetic spatialities. Their application is expected to provide empirical support to the theoretically hypothesised, strong correlation between the increasing dis-embedding strength of territorial infrastructure and the emergence of networked “representational” re-combinations occurring with the latest ‘malling’ form.
- Published
- 2017
- Full Text
- View/download PDF
4. Therapy-resistant dry itchy eyes
- Author
-
Wardeh, Rima, Besgen, Volker, and Sekundo, Walter
- Published
- 2019
- Full Text
- View/download PDF
5. Aphake Artisan/Verisyse-Intraokularlinsen als retropupillare Implantate: Videobeitrag
- Author
-
Besgen, V. and Sekundo, W.
- Published
- 2017
- Full Text
- View/download PDF
6. Electroretinogram and visual field changes in a case of birdshot chorioretinopathy
- Author
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Elbaz, Hisham, Besgen, Volker, Rechberger, Klara, Sekundo, Walter, and Apfelstedt-Sylla, Eckart
- Published
- 2017
- Full Text
- View/download PDF
7. To Find Is To Choose, To Choose Is To Create: Culture(s) in Cinematic and Architectural Space(s)
- Author
-
Asu Besgen
- Subjects
Visual arts ,N1-9211 ,Communication. Mass media ,P87-96 - Abstract
In Wikipedia, which is the symbol of global and virtual source in today’s research culture, culture is defined in three different ways: It is the excellence of taste in fine arts and humanities; an integrated pattern of human knowledge, belief, and behavior that depends upon the capacity for symbolic thought and social learning; and, the set of shared attitudes, values, goals, and practices that characterizes an institution, organization or group. With another generalization, culture is the evolved human capacity to classify and represent experiences with symbols, and to act imaginatively and creatively; moreover, it is the distinct ways that people living in different parts of the world classified and represented their experiences.
- Published
- 2012
- Full Text
- View/download PDF
8. Vorderabschnittbefund mit weitreichenden Folgen
- Author
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Besgen, V., Seipelt, P., and Wenner, Y.
- Published
- 2016
- Full Text
- View/download PDF
9. Zur Versorgungssituation und konservativen Therapie des okulären vernarbenden Schleimhautpemphigoids in Deutschland
- Author
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Yaïci, Rémi, Roth, Mathias, Juergens, Lukas, Al Nawaiseh, Sami, Burkhard, Dick, Besgen, Volker, Fuest, Matthias, Girbardt, Christian, Hampel, Ulrike, Heichel, Jens, Heiligenhaus, Arnd, Herwig-Carl, Martina C, Kakkassery, Vinodh, Kontopoulou, Kalliopi, Löffler, Karin U, Maier, Philip Christian, Nölle, Bernhard, Pach, Johanna, Paul, Sebastian, Pleyer, Uwe, Pöllmann, Michael, Saeger, Mark, Schmidt, Enno, Siebelmann, Sebastian, Sokolenko, Ekatarina, Strudel, Lisa, Stübiger, Nicole, Tarhan, Melih, Theuersbacher, Johanna, van Oterendorp, Christian, Walker, Marten, Wiecha, Carolin, Wykrota, Agata Anna, and Geerling, Gerd
- Abstract
Hintergrund:Die okuläre Beteiligung bei vernarbendem Schleimhautpemphigoid (SHP) ist mit einer Prävalenz von 25 Fällen je 1 Mio. Einwohner und damit ca. 2100 Patienten in ganz Deutschland selten. Die Diagnosestellung kann – besonders in Abwesenheit anderer Beteiligungen – schwierig und die Therapie komplex und langwierig sein. Nicht selten kommen Immunsuppressiva zum Einsatz. Aufgrund der Komplexität von Diagnose und Therapie sind SHP-Patienten meist an entsprechend spezialisierte Zentren angebunden. Ziel dieses Projektes war die Erfassung der aktuellen augenärztlichen Versorgungssituation von Patienten mit SHP in Deutschland. Methoden:Eine papierbasierte Umfrage wurde konzipiert und im April 2020 an alle Universitätsaugenkliniken und weitere potenzielle Zentren versandt. Gefragt wurde nach dem Bestehen einer spezialisierten Sprechstunde, der jährlichen Gesamtzahl der betreuten Patienten, der jährlichen Anzahl von neu diagnostizierten Patienten, den klinischen Kooperationspartnern in Diagnostik und Therapie sowie nach der angewendeten lokalen und systemischen Therapie. Ergebnisse:Von insgesamt 44 angeschrieben Kliniken erfolgten 28 (64%) vollständige Rückmeldungen. Im Mittel werden in den Kliniken 27 ± 42 (0–200) Patienten betreut und jährlich pro Zentrum 3,6 ± 2,2 (0–10) neue Fälle diagnostiziert. Dies entspricht einer Gesamtpatientenzahl von 741 Patienten. Lediglich 9 (32%) der antwortenden Kliniken bieten eine spezialisierte SHP-Sprechstunde an. 93% der Zentren kooperieren mit der lokalen Klinik für Dermatologie. 79% führen die serologische und histologische Diagnostik intern durch. Etwa die Hälfte der Zentren (n = 16) wendet ein standardisiertes Therapieschema an. Systemisch werden Glukokortikoide (66,7%) am häufigsten verwendet, gefolgt von Mycophenolatmofetil und Dapson (57,1 %), Rituximab (33,3%), Azathioprin und Cyclophosphamid (28,6%) sowie Methotrexat (19,0%). Am seltensten werden i.v. Immunoglobuline eingesetzt (14,3 %). Schlussfolgerung:Mit dieser Umfrage unter deutschen augenärztlichen Zentren wurden Daten von etwa einem Drittel der geschätzten Gesamtzahl aller in Deutschland an einem SHP erkrankten Menschen erhoben. Dabei handelt es sich vermutlich ausschließlich um Patienten mit mindestens einer okulären Beteiligung. Aktuell wird eine augenärztliche SHP-Registerstudie etabliert, um die Epidemiologie und Versorgungssituation besser zu erfassen und langfristig zu verbessern.
- Published
- 2023
- Full Text
- View/download PDF
10. Analysis of the paired TCR α- and β-chains of single human T cells.
- Author
-
Song-Min Kim, Latika Bhonsle, Petra Besgen, Jens Nickel, Anna Backes, Kathrin Held, Sigrid Vollmer, Klaus Dornmair, and Joerg C Prinz
- Subjects
Medicine ,Science - Abstract
Analysis of the paired i.e. matching TCR α- and β-chain rearrangements of single human T cells is required for a precise investigation of clonal diversity, tissue distribution and specificity of protective and pathologic T-cell mediated immune responses. Here we describe a multiplex RT-PCR based technology, which for the first time allows for an unbiased analysis of the complete sequences of both α- and β-chains of TCR from single T cells. We validated our technology by the analysis of the pathologic T-cell infiltrates from tissue lesions of two T-cell mediated autoimmune diseases, psoriasis vulgaris (PV) and multiple sclerosis (MS). In both disorders we could detect various T cell clones as defined by multiple T cells with identical α- and β-chain rearrangements distributed across the tissue lesions. In PV, single cell TCR analysis of lesional T cells identified clonal CD8(+) T cell expansions that predominated in the epidermis of psoriatic plaques. An MS brain lesion contained two dominant CD8(+) T-cell clones that extended over the white and grey matter and meninges. In both diseases several clonally expanded T cells carried dual TCRs composed of one Vβ and two different Vα-chain rearrangements. These results show that our technology is an efficient instrument to analyse αβ-T cell responses with single cell resolution in man. It should facilitate essential new insights into the mechanisms of protective and pathologic immunity in many human T-cell mediated conditions and allow for resurrecting functional TCRs from any αβ-T cell of choice that can be used for investigating their specificity.
- Published
- 2012
- Full Text
- View/download PDF
11. Die läsionale T-Zell-Antwort der Psoriasis ist klonal und gegen HLA-Cw6-präsentierte Autoantigene gerichtet: FV08/03
- Author
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Kim, S M, Siewert, K, Besgen, P, Nickel, J, Vollmer, S, Dornmair, K, and Prinz, J C
- Published
- 2011
12. Streptococcal infection distinguishes different types of psoriasis
- Author
-
Weisenseel, P, Laumbacher, B, Besgen, P, Ludolph-Hauser, D, Herzinger, T, Roecken, M, Wank, R, and Prinz, J C
- Published
- 2002
13. 310 Unopposed IL-36 activity promotes clonal CD4+ T-cell responses with IL-17A production in generalized pustular psoriasis
- Author
-
Arakawa, A., primary, Vollmer, S., additional, Besgen, P., additional, Summer, B., additional, Ruzicka, T., additional, Thomas, P., additional, and Prinz, J.C., additional
- Published
- 2017
- Full Text
- View/download PDF
14. 281 Generalized pustular psoriasis combines signs of autoinflammation and antigen-driven Th17 responses
- Author
-
Arakawa, A., primary, Vollmer, S., additional, Besgen, P., additional, Galinski, A., additional, Summer, B., additional, Thomas, P., additional, Spannagl, M., additional, Ruzicka, T., additional, and Prinz, J.C., additional
- Published
- 2016
- Full Text
- View/download PDF
15. 284 HLA-C*06:02 mediates an autoimmune response against melanocytes in psoriasis
- Author
-
Arakawa, A., primary, Siewert, K., additional, Stöhr, J., additional, Besgen, P., additional, Vollmer, S., additional, Thomas, P., additional, Dornmair, K., additional, and Prinz, J.C., additional
- Published
- 2016
- Full Text
- View/download PDF
16. Unopposed IL-36 Activity Promotes Clonal CD4+T-Cell Responses with IL-17A Production in Generalized Pustular Psoriasis
- Author
-
Arakawa, Akiko, Vollmer, Sigrid, Besgen, Petra, Galinski, Adrian, Summer, Burkhard, Kawakami, Yoshio, Wollenberg, Andreas, Dornmair, Klaus, Spannagl, Michael, Ruzicka, Thomas, Thomas, Peter, and Prinz, Jörg C.
- Abstract
Generalized pustular psoriasis (GPP) is the most severe psoriasis variant. Mutations in the IL-36 antagonist IL36RN, in CARD14or AP1S3provide genetic evidence for autoinflammatory etiology but cannot explain its pathogenesis completely. Here we demonstrate that unopposed IL-36 signaling promotes antigen-driven and likely pathogenic T-helper type 17 (Th17) responses in GPP. We observed that CD4+T cells in blood and skin lesions of GPP patients were characterized by intense hyperproliferation, production of the GPP key mediator, IL-17A, and highly restricted TCR repertoires with identical T-cell clones in blood and skin lesions, indicating antigen-driven T-cell expansions. The clonally expanded CD4+T cells were major producers of IL-17A. IL-36 signaling substantially enhanced TCR-mediated proliferation of CD4+T cells. Moreover, GPP patients showed preferences for HLA-DRB1∗14, HLA-DQB1∗05, and HLA-DQB1∗03. We conclude that in GPP unopposed IL-36 signaling and certain HLA-class II alleles may cooperate in promoting antigen-driven Th17 responses, which in the obvious absence of exogenous triggers may reflect autoimmune reactions. This study reveals a pathogenic pathway where innate immune dysregulation promotes T-cell−mediated inflammation in GPP.
- Published
- 2018
- Full Text
- View/download PDF
17. Melanocyte antigen triggers autoimmunity in human psoriasis
- Author
-
Arakawa, Akiko, Siewert, Katherina, Stöhr, Julia, Besgen, Petra, Kim, Song-Min, Rühl, Geraldine, Nickel, Jens, Vollmer, Sigrid, Thomas, Peter, Krebs, Stefan, Pinkert, Stefan, Spannagl, Michael, Held, Kathrin, Kammerbauer, Claudia, Besch, Robert, Dornmair, Klaus, and Prinz, Jörg C.
- Abstract
Psoriasis vulgaris is a common T cell–mediated inflammatory skin disease with a suspected autoimmune pathogenesis. The human leukocyte antigen (HLA) class I allele, HLA-C*06:02, is the main psoriasis risk gene. Epidermal CD8+ T cells are essential for psoriasis development. Functional implications of HLA-C*06:02 and mechanisms of lesional T cell activation in psoriasis, however, remained elusive. Here we identify melanocytes as skin-specific target cells of an HLA-C*06:02–restricted psoriatic T cell response. We found that a Vα3S1/Vβ13S1 T cell receptor (TCR), which we had reconstituted from an epidermal CD8+ T cell clone of an HLA-C*06:02–positive psoriasis patient specifically recognizes HLA-C*06:02–positive melanocytes. Through peptide library screening, we identified ADAMTS-like protein 5 (ADAMTSL5) as an HLA-C*06:02–presented melanocytic autoantigen of the Vα3S1/Vβ13S1 TCR. Consistent with the Vα3S1/Vβ13S1-TCR reactivity, we observed numerous CD8+ T cells in psoriasis lesions attacking melanocytes, the only epidermal cells expressing ADAMTSL5. Furthermore, ADAMTSL5 stimulation induced the psoriasis signature cytokine, IL-17A, in CD8+ T cells from psoriasis patients only, supporting a role as psoriatic autoantigen. This unbiased analysis of a TCR obtained directly from tissue-infiltrating CD8+ T cells reveals that in psoriasis HLA-C*06:02 directs an autoimmune response against melanocytes through autoantigen presentation. We propose that HLA-C*06:02 may predispose to psoriasis via this newly identified autoimmune pathway.
- Published
- 2015
- Full Text
- View/download PDF
18. Teaching/Learning Strategies Through Art: Art and Basic Design Education
- Author
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Besgen, Asu, Kuloglu, Nilgun, and Fathalizadehalemdari, Sara
- Abstract
“The ultimate aim of any creative activity is building… architects, sculptors, painters, we all must become craftsmen again… no essential difference exists between the artist and the craftsman, the artist is a craftsman of heightened awareness… but the basis of craftsmanship is indispensable to all artists. It is the prime source of all creative work”, Walter Gropius, from Bauhaus Manifesto of Foundation/Basic Course, 1919.“Basic Design”, also known as “Foundation Courses” or “Enseignement Préliminaire”, formed the basis of the pedagogy of the classical schools of design and architecture. That is, the “Vhutemas”, the “Bauhaus”, the “Chicago Bauhaus”, and the “Ulm School” (Hochschule Fur Gestaltung). This teaching approach, which has undergone multiple changes since the 1920s, lost much of its importance from the 1960s onwards, and at times even disappeared from educational programs in Germany. However, the past 20 years or so have witnessed a rebirth of “Basic Design Education” through many debates and pedagogical propositions (Boucharenc, 2006). In the light of this truth, it can be said that, the basic design has the same importance at all disciplines, although it differentiates on applications in the educations, and this importance is being increased day by day. In the context of this research, especially it will be focused on the basic design education in art and architecture and this concept will be related with these disciplines. The aim of this research is to put forward the right definitions for basic design concept and to evaluate it with relating its own elements and principles, if the design steps are built with a concept, it will be able to see the differences in its properties and right thought, in producing an idea and the effect of composing a concept on defining design power.
- Published
- 2015
- Full Text
- View/download PDF
19. Teaching/Learning Strategies Through Art: Painting and Basic Design Education
- Author
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Besgen, Asu
- Abstract
The art and architecture relationship is discussed within the context of historical process since the first mankind product. Moreover, the art and architecture are mentioned as the most important components through the history of civilisation. Art is a diverse range of human activities and the products of those activities; which depend on the creation of images or objects in fields including painting, sculpture, photography, and other visual media and also architecture. Each artwork is the product of its artist's creativity. The artist forms his artwork through his lifetime experiences in a subjective way by the help of artistic expression, which is as same as for an architect and his architectural work. The art of painting as an art form is the specific shape, or quality an artistic expression takes, which is as same as for an architectural form, and the methods used through for a painting is nearly as same as the methods that an architect uses for his architecture. In the view of this phenomenon, the paper focuses on the relationship between painting and architecture through basic design education. The common elements and principles in painting and architecture will be held in details with examples, to put forward the importance of relationship between two fields, in the aim of proposing a new teaching/learning strategy for basic design education. In the light of this aim, mainly the “space” concept, which is the most common element for an artistic and architectural work will be exemplified with the course practices produced at Karadeniz Technical University, Department of Architecture, Trabzon, Turkey.
- Published
- 2015
- Full Text
- View/download PDF
20. Metamorphism in culture and housing desing: Turkey as an example.
- Author
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Ozdemir, Ilkay Masat and Gencosmanoglu, Asu Besgen
- Subjects
CULTURE ,HOUSING ,SOCIAL change - Abstract
Abstract: Lawrence maintains about all architectural environments and housing that, they are both in a relationship with culture and they are a total configuration of social, demographical, psychological, human behavioral and environmental structure. Moreover, in analyzing this complex structure, he emphasizes to examine it within two perspectives: design-meaning and use. Consequently, the basic components, which affect housing design, are classified in three main topics: cultural, social, and psychological. In the light of Lawrence''s ideas, under the topic of the role of culture and tradition in the development of housing, this paper aims to define the basic Turkish traditional housing principles with slogans and important examples. In this content, cultural, social and psychological components in traditional Turkish houses are held under the heading of the development of traditional Turkish house. The organization rules, the effects of the basic psycho-social components…etc., and the presence of these principles are criticized with Turkish housing examples before and after 1980. The chosen period in this study is not coincidental. The aim of choosing the periods before and after 1980 has a special meaning in Turkey''s economic, politic and social life. Choosing these two basic periods, will not only point out the changes—like a metamorphism—in cultural life, but in architectural needs in Turkish houses. Therefore, in the aim of analyzing cultural changes and their effects on housing design, the architectural meanings in the elements of Turkish houses are put forward in details, in order to make some estimation for the future of changing Turkish architectural life. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
21. Criticizing architectural education through abstraction
- Author
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Gencosmanoglu, Asu Besgen and Nezor, Seda
- Abstract
As a result of the researches conducted so far, it is determined that the individuals who have received architectural education, gain a differentiation in perception, and therefore, the architectural education is somehow directive and distinctive. For this reason, in the paper, it is tried to analyze the differences of the perception and expression of abstraction by the effects of architectural education.
- Published
- 2010
- Full Text
- View/download PDF
22. Learning, Teaching and Administration in Design Education: DESIGNtrain Project: Training Tools for Developing Design Education
- Author
-
Gencosmanoglu, Asu Besgen
- Abstract
The paper targets, not only to introduce DESIGNtrain Project, which was completed within the scope of European Union, Life- Long Learning: Leonardo da Vinci Program, by ten partners from five European countries in 2009, but its aims and results within the topics of learning, teaching and administration in design education, as well.
- Published
- 2010
- Full Text
- View/download PDF
23. CO2-Angiographie: Entwicklung und Erprobung einer tierexperimentell einsetzbaren Gas-Dosiervorrichtung Carbon dioxide Angiography: Development and Testing of a Gas Metering Device for use in Animal Experiments
- Author
-
Krasny, R. and Besgen, J. H.
- Published
- 1990
- Full Text
- View/download PDF
24. C02-Angiographie: Blutflußmessung mit injizierten Gasblasen - Carbon Dioxide Angiography: Blood Flow Measurement with Injected Gas Bubbles
- Author
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Pott, H., Schmitz-Rode, Th., Besgen, J. H., Schirmer, O., and Wintz, T.
- Published
- 1992
- Full Text
- View/download PDF
25. 310 Unopposed IL-36 activity promotes clonal CD4+T-cell responses with IL-17A production in generalized pustular psoriasis
- Author
-
Arakawa, A., Vollmer, S., Besgen, P., Summer, B., Ruzicka, T., Thomas, P., and Prinz, J.C.
- Published
- 2017
- Full Text
- View/download PDF
26. Servopneumatischer Blutpumpenantrieb
- Author
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Backé, W., Kolvenbach, H., and Besgen, J. H.
- Published
- 1988
- Full Text
- View/download PDF
27. Unopposed IL-36 Activity Promotes Clonal CD4 + T-Cell Responses with IL-17A Production in Generalized Pustular Psoriasis.
- Author
-
Arakawa A, Vollmer S, Besgen P, Galinski A, Summer B, Kawakami Y, Wollenberg A, Dornmair K, Spannagl M, Ruzicka T, Thomas P, and Prinz JC
- Subjects
- Adult, Aged, Autoantigens immunology, Female, Histocompatibility Antigens Class II immunology, Histocompatibility Antigens Class II metabolism, Humans, Interleukin-1 immunology, Interleukin-17 immunology, Interleukins genetics, Interleukins immunology, Male, Middle Aged, Psoriasis genetics, Psoriasis pathology, Receptors, Antigen, T-Cell immunology, Receptors, Antigen, T-Cell metabolism, Skin immunology, Skin metabolism, Skin pathology, Th17 Cells metabolism, Young Adult, Autoimmunity, Interleukin-1 metabolism, Interleukin-17 metabolism, Psoriasis immunology, Th17 Cells immunology
- Abstract
Generalized pustular psoriasis (GPP) is the most severe psoriasis variant. Mutations in the IL-36 antagonist IL36RN, in CARD14 or AP1S3 provide genetic evidence for autoinflammatory etiology but cannot explain its pathogenesis completely. Here we demonstrate that unopposed IL-36 signaling promotes antigen-driven and likely pathogenic T-helper type 17 (Th17) responses in GPP. We observed that CD4
+ T cells in blood and skin lesions of GPP patients were characterized by intense hyperproliferation, production of the GPP key mediator, IL-17A, and highly restricted TCR repertoires with identical T-cell clones in blood and skin lesions, indicating antigen-driven T-cell expansions. The clonally expanded CD4+ T cells were major producers of IL-17A. IL-36 signaling substantially enhanced TCR-mediated proliferation of CD4+ T cells. Moreover, GPP patients showed preferences for HLA-DRB1∗14, HLA-DQB1∗05, and HLA-DQB1∗03. We conclude that in GPP unopposed IL-36 signaling and certain HLA-class II alleles may cooperate in promoting antigen-driven Th17 responses, which in the obvious absence of exogenous triggers may reflect autoimmune reactions. This study reveals a pathogenic pathway where innate immune dysregulation promotes T-cell-mediated inflammation in GPP., (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
28. The effect of phototherapy on systemic inflammatory process in patients with plaque psoriasis.
- Author
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Batycka-Baran A, Besgen P, Wolf R, Szepietowski JC, and Prinz JC
- Subjects
- Adult, Aged, Cytokines genetics, Cytokines metabolism, Female, Humans, Inflammation prevention & control, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear metabolism, Leukocytes, Mononuclear radiation effects, Male, Middle Aged, Psoriasis metabolism, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction, Ultraviolet Rays, Phototherapy, Psoriasis therapy
- Abstract
Psoriasis is a common, chronic immune-mediated inflammatory disease. The inflammatory process in psoriasis has systemic effects and may influence the development of psoriatic comorbidities. The systemic action of phototherapy in patients with psoriasis has been so far poorly elucidated. We aimed to investigate the expression of genes encoding selected psoriasis-related cytokines in peripheral blood mononuclear cells (PBMCs) isolated from patients with psoriasis before and after treatment with phototherapy. 17 patients with mild to moderate plaque psoriasis were treated with narrow band-UVB (NB-UVB), 8 patients with moderate to severe plaque psoriasis with bath-psoralen-ultraviolet A therapy (PUVA). PBMCs were isolated by Ficoll gradient density centrifugation. Expression of genes encoding TNF-α, IL-17A, IL-6, IL-1 β, INF-γ, and IL-10 in PBMCs of patients with psoriasis before and after phototherapy was analyzed with quantitative RT-PCR. Treatment with NB-UVB therapy led to a significant decrease in IL-17A, TNF-α, and IL-6 mRNA levels in PBMCs (p=0.003; p=0.042; p=0.019, respectively). Following treatment with bath-PUVA therapy, we observed a significant decrease in TNF-α and IL-6 mRNA levels in PBMCs (p=0.031, p=0.035, respectively). Treatment with phototherapy in patients with psoriasis may affect systemic inflammation by downregulation of the expression of genes encoding proinflammatory cytokines in PBMCs, implicated in the development of psoriasis and psoriatic comorbidities., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
29. Analysis of the paired TCR α- and β-chains of single human T cells.
- Author
-
Kim SM, Bhonsle L, Besgen P, Nickel J, Backes A, Held K, Vollmer S, Dornmair K, and Prinz JC
- Subjects
- Antigen-Presenting Cells immunology, DNA Primers genetics, Flow Cytometry, Humans, Multiple Sclerosis immunology, Psoriasis immunology, Skin immunology, Genes, T-Cell Receptor alpha genetics, Genes, T-Cell Receptor beta genetics, Multiplex Polymerase Chain Reaction methods, Reverse Transcriptase Polymerase Chain Reaction methods, T-Lymphocytes metabolism
- Abstract
Analysis of the paired i.e. matching TCR α- and β-chain rearrangements of single human T cells is required for a precise investigation of clonal diversity, tissue distribution and specificity of protective and pathologic T-cell mediated immune responses. Here we describe a multiplex RT-PCR based technology, which for the first time allows for an unbiased analysis of the complete sequences of both α- and β-chains of TCR from single T cells. We validated our technology by the analysis of the pathologic T-cell infiltrates from tissue lesions of two T-cell mediated autoimmune diseases, psoriasis vulgaris (PV) and multiple sclerosis (MS). In both disorders we could detect various T cell clones as defined by multiple T cells with identical α- and β-chain rearrangements distributed across the tissue lesions. In PV, single cell TCR analysis of lesional T cells identified clonal CD8(+) T cell expansions that predominated in the epidermis of psoriatic plaques. An MS brain lesion contained two dominant CD8(+) T-cell clones that extended over the white and grey matter and meninges. In both diseases several clonally expanded T cells carried dual TCRs composed of one Vβ and two different Vα-chain rearrangements. These results show that our technology is an efficient instrument to analyse αβ-T cell responses with single cell resolution in man. It should facilitate essential new insights into the mechanisms of protective and pathologic immunity in many human T-cell mediated conditions and allow for resurrecting functional TCRs from any αβ-T cell of choice that can be used for investigating their specificity.
- Published
- 2012
- Full Text
- View/download PDF
30. Ezrin, maspin, peroxiredoxin 2, and heat shock protein 27: potential targets of a streptococcal-induced autoimmune response in psoriasis.
- Author
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Besgen P, Trommler P, Vollmer S, and Prinz JC
- Subjects
- Amino Acid Sequence, Animals, Autoantigens immunology, Autoimmune Diseases blood, Cell Line, Transformed, Cell Line, Tumor, Cells, Cultured, Cytoskeletal Proteins blood, Epitopes, T-Lymphocyte immunology, HSP27 Heat-Shock Proteins blood, Humans, Keratinocytes immunology, Keratinocytes metabolism, Keratinocytes pathology, Molecular Sequence Data, Peroxiredoxins blood, Psoriasis blood, Psoriasis microbiology, Rabbits, Serpins blood, Streptococcal Infections blood, Streptococcal Vaccines administration & dosage, Streptococcal Vaccines immunology, Streptococcus pyogenes immunology, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets microbiology, Autoimmune Diseases immunology, Autoimmune Diseases microbiology, Cytoskeletal Proteins immunology, HSP27 Heat-Shock Proteins immunology, Peroxiredoxins immunology, Psoriasis immunology, Serpins immunology, Streptococcal Infections immunology
- Abstract
Psoriasis is an HLA-Cw6-associated T cell-mediated autoimmune disease of the skin that is often triggered by streptococcal angina. To identify keratinocyte proteins, which may become psoriatic autoantigens as the result of an immune response against streptococci, rabbits were immunized with heat-killed Streptococcus pyogenes. Streptococcal immunization induced Ab formation against various human keratinocyte proteins. Sera from psoriasis patients reacted against several of these proteins as well. Common serologic reactivities of rabbits and patients included the proteins ezrin, maspin, peroxiredoxin 2 (PRDX2), heat shock protein (hsp)27, and keratin 6. When used for stimulation of blood lymphocytes, ezrin, maspin, PRDX2, and hsp27 induced increased T cell activation in psoriasis patients, which was particularly evident for HLA-Cw6(+) individuals. Ag-specific T cell lines generated with these proteins consisted predominantly of CD8(+) T cells and used TCR beta-chain rearrangements, which were highly homologous to those expanded within the corresponding skin lesion. Several immunodominant epitopes on the different proteins could be defined according to sequence alignments with the whole genome of S. pyogenes. Our data indicate that maspin, ezrin, PRDX2, hsp27, and potentially keratin 6 could act as autoantigens of a streptococcal-induced autoimmune response and represent targets of the exaggerated T cell response in psoriasis. Additionally, ezrin and hsp27 might constitute antigenic links between psoriasis and inflammatory bowel disease, uveitis, or arteriosclerosis, which are clinically associated.
- Published
- 2010
- Full Text
- View/download PDF
31. Identical TCR beta-chain rearrangements in streptococcal angina and skin lesions of patients with psoriasis vulgaris.
- Author
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Diluvio L, Vollmer S, Besgen P, Ellwart JW, Chimenti S, and Prinz JC
- Subjects
- Adult, Amino Acid Sequence, Antigens, Differentiation, T-Lymphocyte, Antigens, Neoplasm genetics, Base Sequence, Clone Cells, Humans, Membrane Glycoproteins genetics, Molecular Sequence Data, Multigene Family, Palatine Tonsil immunology, Palatine Tonsil metabolism, Palatine Tonsil pathology, Psoriasis surgery, Receptors, Antigen, T-Cell, alpha-beta genetics, Receptors, Lymphocyte Homing genetics, Remission Induction, Rheumatic Heart Disease pathology, Rheumatic Heart Disease surgery, Severity of Illness Index, Streptococcal Infections pathology, Streptococcal Infections surgery, Streptococcus pyogenes immunology, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets pathology, Tonsillectomy, Tonsillitis immunology, Tonsillitis pathology, Tonsillitis surgery, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Psoriasis immunology, Psoriasis pathology, Receptors, Antigen, T-Cell, alpha-beta biosynthesis, Receptors, Antigen, T-Cell, alpha-beta isolation & purification, Rheumatic Heart Disease immunology, Streptococcal Infections immunology, T-Lymphocyte Subsets metabolism
- Abstract
Tonsillar infection with Streptococcus pyogenes may induce several nonsuppurative autoimmune sequelae. The precise pathogenetic mechanisms behind this clinically well-established association are still unresolved. Using TCR analysis, we sought to identify a link between streptococcal tonsillitis and the T cell-mediated autoimmune response in psoriasis. Three patients with streptococcal-induced psoriasis underwent tonsillectomy. Using size spectratyping and sequencing of TCR beta-chain variable region gene (TCRBV) rearrangements, we compared the TCR usage of psoriatic skin lesions, blood, tonsils, and tonsillar T cells fractionated according to the expression of the skin address in "cutaneous lymphocyte-associated Ag" (CLA). TCRBV-size spectratype analysis of the blood lymphocytes, tonsils, and the CLA-negative tonsillar T cells revealed largely unselected T cell populations. Instead, TCRBV gene families of the psoriatic lesions and skin-homing CLA-positive tonsillar T cells displayed highly restricted spectratypes. Sequencing of TCRBV cDNA identified various clonal TCRBV rearrangements within the psoriatic lesions that indicated Ag-driven T cell expansion. Several of these clonotypes were also detected within the tonsils and, in one of the patients, within the small subset of CLA-positive tonsillar T cells, suggesting that T cells from the same T cell clones were simultaneously present within skin and tonsillar tissue. Because after tonsillectomy psoriasis cleared in all three patients our observations indicate that T cells may connect psoriatic inflammation to streptococcal angina. They suggest that the chronic streptococcal immune stimulus within the tonsils could act as a source for pathogenic T cells in poststreptococcal disorders, and they may help to explain why eliminating this source with tonsillectomy may improve streptococcal-induced sequelae.
- Published
- 2006
- Full Text
- View/download PDF
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