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Identical TCR beta-chain rearrangements in streptococcal angina and skin lesions of patients with psoriasis vulgaris.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2006 Jun 01; Vol. 176 (11), pp. 7104-11. - Publication Year :
- 2006
-
Abstract
- Tonsillar infection with Streptococcus pyogenes may induce several nonsuppurative autoimmune sequelae. The precise pathogenetic mechanisms behind this clinically well-established association are still unresolved. Using TCR analysis, we sought to identify a link between streptococcal tonsillitis and the T cell-mediated autoimmune response in psoriasis. Three patients with streptococcal-induced psoriasis underwent tonsillectomy. Using size spectratyping and sequencing of TCR beta-chain variable region gene (TCRBV) rearrangements, we compared the TCR usage of psoriatic skin lesions, blood, tonsils, and tonsillar T cells fractionated according to the expression of the skin address in "cutaneous lymphocyte-associated Ag" (CLA). TCRBV-size spectratype analysis of the blood lymphocytes, tonsils, and the CLA-negative tonsillar T cells revealed largely unselected T cell populations. Instead, TCRBV gene families of the psoriatic lesions and skin-homing CLA-positive tonsillar T cells displayed highly restricted spectratypes. Sequencing of TCRBV cDNA identified various clonal TCRBV rearrangements within the psoriatic lesions that indicated Ag-driven T cell expansion. Several of these clonotypes were also detected within the tonsils and, in one of the patients, within the small subset of CLA-positive tonsillar T cells, suggesting that T cells from the same T cell clones were simultaneously present within skin and tonsillar tissue. Because after tonsillectomy psoriasis cleared in all three patients our observations indicate that T cells may connect psoriatic inflammation to streptococcal angina. They suggest that the chronic streptococcal immune stimulus within the tonsils could act as a source for pathogenic T cells in poststreptococcal disorders, and they may help to explain why eliminating this source with tonsillectomy may improve streptococcal-induced sequelae.
- Subjects :
- Adult
Amino Acid Sequence
Antigens, Differentiation, T-Lymphocyte
Antigens, Neoplasm genetics
Base Sequence
Clone Cells
Humans
Membrane Glycoproteins genetics
Molecular Sequence Data
Multigene Family
Palatine Tonsil immunology
Palatine Tonsil metabolism
Palatine Tonsil pathology
Psoriasis surgery
Receptors, Antigen, T-Cell, alpha-beta genetics
Receptors, Lymphocyte Homing genetics
Remission Induction
Rheumatic Heart Disease pathology
Rheumatic Heart Disease surgery
Severity of Illness Index
Streptococcal Infections pathology
Streptococcal Infections surgery
Streptococcus pyogenes immunology
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets pathology
Tonsillectomy
Tonsillitis immunology
Tonsillitis pathology
Tonsillitis surgery
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
Psoriasis immunology
Psoriasis pathology
Receptors, Antigen, T-Cell, alpha-beta biosynthesis
Receptors, Antigen, T-Cell, alpha-beta isolation & purification
Rheumatic Heart Disease immunology
Streptococcal Infections immunology
T-Lymphocyte Subsets metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 176
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 16709873
- Full Text :
- https://doi.org/10.4049/jimmunol.176.11.7104