46 results on '"Belloy, M."'
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2. Dépistage néonatal ciblé de la drépanocytose : bilan de cinq années d’expérience dans le nord-francilien
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Ducrocq, R, Benkerrou, M, Brahimi, L, Belloy, M, Briard, M.L, Vilmer, E, and Elion, J
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- 2001
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3. Erythroid Hypoplasia Due to Chronic Infection with Parvovirus B19
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Belloy, M., Morinet, F., Blondin, G., Courouce, A. M., Peyrol, Y., and Vilmer, E.
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- 1990
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4. Infection bactérienne de grade 4 transmise par transfusion
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Hauser, L., primary, Menasie, S., additional, Raoult, L., additional, Ait Oubelli, N., additional, Belloy, M., additional, Avran, D., additional, Beyloune, A., additional, Simonet, M., additional, Pangon, B., additional, Bierling, P., additional, and Bonacorsi, S., additional
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- 2015
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5. Altérations de la structure épithéliale des cellules tubulaires rénales en réponse au shear stress
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Maggiorani, D., primary, Belloy, M., additional, Casemayou, A., additional, Dissard, R., additional, Bellière, J., additional, Caubet, C., additional, Bascands, J.L., additional, Schanstra, J., additional, and Buffin-Meyer, B., additional
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- 2014
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6. P-190 – Papillomavirus Humain et vaccination: que savent les adolescents? Parcours prédiagnostique des enfants et adolescents atteints de cancer
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Tatencloux, S., Mosseri, V., Papillard-Maréchal, S., Mesples, B., Pellegrino, B., Belloy, M., Jimenez, I., Algret, N., Levy, D., Michon, J., and Orbach, D.
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- 2015
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7. Hétérogénéité génotypique du déficit en G6PD à l’Ouest de l’Afrique Sub-saharienne
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Messie, K., Pasquaud, O., Benkerrou, Malika, Belloy, M., Lahary, A., Cotton, Frédéric, Elion, J., Ducrocq, R., Messie, K., Pasquaud, O., Benkerrou, Malika, Belloy, M., Lahary, A., Cotton, Frédéric, Elion, J., and Ducrocq, R.
- Abstract
Congrès 2000 de la Société Française d’Hématologie – Paris 12-14/03/2000, info:eu-repo/semantics/published
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- 2000
8. Hétérogénéité génotypique du déficit en G6PD à l’Ouest de l’Afrique Sub-saharienne
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Congrès 2000 de la Société Française d’Hématologie (12-14/03/2000: Paris), Messie, K., Pasquaud, O., Benkerrou, Malika, Belloy, M., Lahary, A., Cotton, Frédéric, Elion, J., Ducrocq, R., Congrès 2000 de la Société Française d’Hématologie (12-14/03/2000: Paris), Messie, K., Pasquaud, O., Benkerrou, Malika, Belloy, M., Lahary, A., Cotton, Frédéric, Elion, J., and Ducrocq, R.
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Hématologie 2000; 6(HS):45, info:eu-repo/semantics/nonPublished
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- 2000
9. Maladie de Kawasaki et dengue : association fortuite ?
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Tourneux, P., primary, Dufillot, D., additional, Boussemart, T., additional, and Belloy, M., additional
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- 2002
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10. Three-Year Follow-Up of Hydroxyurea Treatment in Severely Ill Children with Sickle Cell Disease
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de Montalembert, M., primary, Belloy, M., additional, Bernaudin, F., additional, Gouraud, F., additional, Capdeville, R., additional, Mardini, R., additional, Philippe, N., additional, Jais, J. P., additional, Bardakdjian, J., additional, Ducrocq, R., additional, Maier-Redelsperger, M., additional, Elion, J., additional, Labie, D., additional, and Girot, R., additional
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- 1997
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11. Prévention et traitement des troubles érectiles de la drépanocytose
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Bachir, D, primary, Virag, R, additional, Lee, K, additional, Belloy, M, additional, de Montalembert, M, additional, Denis, L, additional, Broyart, A, additional, Girot, R, additional, and Galactéros, F, additional
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- 1997
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12. Traitement curatif et préventif du priapisme dans la drépanocytose avec l'étiléfrine oral et intracaverneux
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Bachir, D, primary, Virag, R, additional, Lee, K, additional, Godeau, B, additional, Belloy, M, additional, and Galactéros, F, additional
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- 1995
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13. Beta-globin gene cluster haplotype and alpha-thalassemia do not correlate with the acute clinical manifestations of sickle cell disease in children [letter]
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de Montalembert, M, primary, Maier-Redelsperger, M, additional, Girot, R, additional, Belloy, M, additional, Vilmer, E, additional, Ducrocq, R, additional, Guidal, C, additional, and Elion, J, additional
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- 1993
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14. The relative importance of the X-linked FCP locus and beta-globin haplotypes in determining haemoglobin F levels: a study of SS patients homozygous for betaS haplotypes
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Chang, Y.P.C., Maier-Redelsperger, M., Smith, K.D., Contu, L., Ducrocq, R., Montalembert, M. de, Belloy, M., Elion, J., Dover, G. J, and Girot, R.
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Five factors have been hypothesized to influence the 20-fold variation in fetal haemoglobin (Hb F) levels in sickle cell anaemia (SS): age sex, alpha-globin gene number, beta-globin haplotype, and the X-linked F-cell production locus (FCP) that regulates the production of Hb F containing erythrocytes (F cells). We analysed the association of these factors with Hb F levels in 112 SS patients living in France who are homozygous for the three common African beta-globin haplotypes (Benin, Bantu or Central African Republic and Senegal). We found that: (1) FCP accounts for about 40% of the overall variation in Hb F levels, (2) when the FCP influence is removed, beta-globin haplotype is associated with 14% of the remaining Hb F variation, and (3) the other factors have little influence. Comparison with our previous study of SS individuals in Jamaica leads to the following conclusions: (1) the X-linked FCP locus is a major determinant of Hb F levels in SS disease, (2) factors linked to the beta-globin haplotype have only a small effect on the variation in Hb F levels, in either the homozygous or heterozygous state, and (3) approximately half of the variation in Hb F levels still remains to be explained.
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- 1997
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15. Variation in fetal hemoglobin parameters and predicted hemoglobin S polymerization in sickle cell children in the first two years of life: Parisian prospective study on sickle cell disease
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Maier-Redelsperger, M., Noguchi, C. T., Montalembert, M., Rodgers, G. P., Alan N Schechter, Gourbil, A., Blanchard, D., Jais, J. P., Ducrocq, R., Peltier, J. -Y, Cottat, M. -C, Lacaille, F., Belloy, M., Elion, J., Labie, D., and Girot, R.
16. Childhood sickle cell crises: clinical severity, inflammatory markers and the role of interleukin-8
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Maryse ETIENNE-JULAN, Belloy, M. -S, Decastel, M., Dougaparsad, S., Ravion, S., and Hardy-Dessourees, M. -D
17. Titus : tragédie en cinq actes, avec des observations sur la poësie dramatique adressées à M. de Voltaire.
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Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775. and Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775.
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Title vignette; head- and tailpieces.
18. Le siège de Calais : tragédie / par M. de Belloy ; ... suivie de notes historiques.
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Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775. and Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775.
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In verse. Représentée pour la première fois, par les comédiens français ordinaires du roi, le 13 février 1765. Signatures: a? A-G? H?. Publisher's advertisements: p. [2] at end.
19. Le siège de Calais : tragédie ... / par M. de Belloy; représentée pour la première fois, par les Comédiens français ordinaires du Roi, le 13 fèvrier 1765 ; suivie de notes historiques.
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Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775. and Belloy, M. de (Pierre-Laurent Buyrette), 1727-1775.
20. Association of Rare APOE Missense Variants V236E and R251G with Risk of Alzheimer Disease
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Le Guen, Yann, Belloy, Michael E, Eger, Sarah J, Mir, Pablo, Moreno, Fermin, Pastor, Pau, Piñol-Ripoll, Gerard, Molina-Porcel, Laura, Pérez-Tur, Jordi, Rodríguez-Rodríguez, Eloy, Royo, Jose Luís, Sánchez-Valle, Raquel, Dichgans, Martin, Rasmussen, Katrine Laura, Rujescu, Dan, Thomassen, Jesper Qvist, Deleuze, Jean-François, He, Zihuai, Napolioni, Valerio, Amouyel, Philippe, Jessen, Frank, Kehoe, Patrick Gavin, van Duijn, Cornelia, Grenier-Boley, Benjamin, Tsolaki, Magda, Sánchez-Juan, Pascual, Sleegers, Kristel, Ingelsson, Martin, Rossi, Giacomina, Hiltunen, Mikko, Sims, Rebecca, van der Flier, Wiesje M, Ramirez, Alfredo, Andreassen, Ole A, de Rojas, Itziar, Frikke-Schmidt, Ruth, Williams, Julie, Ruiz, Agustín, Lambert, Jean-Charles, Greicius, Michael D, Members of the EADB, GR@ACE, DEGESCO, DemGene, GERAD, Groups, EADI, Arosio, Beatrice, Benussi, Luisa, Boland, Anne, Castillo-Morales, Atahualpa, Borroni, Barbara, Caffarra, Paolo, Daian, Delphine, Daniele, Antonio, Debette, Stéphanie, Dufouil, Carole, Düzel, Emrah, Galimberti, Daniela, Giedraitis, Vilmantas, Grimmer, Timo, Jansen, Iris, Graff, Caroline, Grünblatt, Edna, Hanon, Olivier, Hausner, Lucrezia, Heilmann-Heimbach, Stefanie, Holstege, Henne, Hort, Jakub, Jürgen, Deckert, Kuulasmaa, Teemu, van der Lugt, Aad, Nicolas, Aude, Masullo, Carlo, Mecocci, Patrizia, Mehrabian, Shima, de Mendonça, Alexandre, Moebus, Susanne, Nacmias, Benedetta, Nicolas, Gael, Olaso, Robert, Papenberg, Goran, Parnetti, Lucilla, Bellenguez, Céline, Pasquier, Florence, Peters, Oliver, Pijnenburg, Yolande A L, Popp, Julius, Rainero, Innocenzo, Ramakers, Inez, Riedel-Heller, Steffi, Scarmeas, Nikolaos, Scheltens, Philip, Scherbaum, Norbert, Dalmasso, Carolina, Schneider, Anja, Seripa, Davide, Soininen, Hilkka, Solfrizzi, Vincenzo, Spalletta, Gianfranco, Squassina, Alessio, van Swieten, John, Tegos, Thomas J, Tremolizzo, Lucio, Verhey, Frans, Küçükali, Fahri, Vyhnalek, Martin, Wiltfang, Jens, Boada, Mercè, García-González, Pablo, Puerta, Raquel, Real, Luis M, Álvarez, Victoria, Bullido, María J, Clarimon, Jordi, García-Alberca, José María, Neurology, Amsterdam Neuroscience - Neurodegeneration, VU University medical center, APH - Personalized Medicine, APH - Methodology, National Institutes of Health (US), National Institute on Aging (US), European Research Council, Pérez-Tur, Jordi [0000-0002-9111-1712], RS: MHeNs - R1 - Cognitive Neuropsychiatry and Clinical Neuroscience, Psychology 2, Psychiatrie & Neuropsychologie, MUMC+: MA Med Staf Spec Psychiatrie (9), Le Guen, Y, Belloy, M, Grenier-Boley, B, de Rojas, I, Castillo-Morales, A, Jansen, I, Nicolas, A, Bellenguez, C, Dalmasso, C, Küçükali, F, Eger, S, Rasmussen, K, Thomassen, J, Deleuze, J, He, Z, Napolioni, V, Amouyel, P, Jessen, F, Kehoe, P, van Duijn, C, Tsolaki, M, Sánchez-Juan, P, Sleegers, K, Ingelsson, M, Rossi, G, Hiltunen, M, Sims, R, van der Flier, W, Ramirez, A, Andreassen, O, Frikke-Schmidt, R, Williams, J, Ruiz, A, Lambert, J, Greicius, M, Arosio, B, Benussi, L, Boland, A, Borroni, B, Caffarra, P, Daian, D, Daniele, A, Debette, S, Dufouil, C, Düzel, E, Galimberti, D, Giedraitis, V, Grimmer, T, Graff, C, Grünblatt, E, Hanon, O, Hausner, L, Heilmann-Heimbach, S, Holstege, H, Hort, J, Jürgen, D, Kuulasmaa, T, van der Lugt, A, Masullo, C, Mecocci, P, Mehrabian, S, de Mendonça, A, Moebus, S, Nacmias, B, Nicolas, G, Olaso, R, Papenberg, G, Parnetti, L, Pasquier, F, Peters, O, Pijnenburg, Y, Popp, J, Rainero, I, Ramakers, I, Riedel-Heller, S, Scarmeas, N, Scheltens, P, Scherbaum, N, Schneider, A, Seripa, D, Soininen, H, Solfrizzi, V, Spalletta, G, Squassina, A, van Swieten, J, Tegos, T, Tremolizzo, L, Verhey, F, Vyhnalek, M, Wiltfang, J, Boada, M, García-González, P, Puerta, R, Real, L, Álvarez, V, Bullido, M, Clarimon, J, García-Alberca, J, Mir, P, Moreno, F, Pastor, P, Piñol-Ripoll, G, Molina-Porcel, L, Pérez-Tur, J, Rodríguez-Rodríguez, E, Royo, J, Sánchez-Valle, R, Dichgans, M, Rujescu, D, Epidemiology, Radiology & Nuclear Medicine, Graduate School, Medical Psychology, APH - Quality of Care, Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), EADB Group, GR@ACE Groupp, DEGESCO Group, DemGene Group, GERAD Group, EADI Group, Repositório da Universidade de Lisboa, and Pérez-Tur, Jordi
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Male ,Genotype ,Apolipoprotein E2 ,epidemiology [Alzheimer Disease] ,Apolipoprotein E4 ,Medizin ,genetics [Alzheimer Disease] ,APOLIPOPROTEIN-E ,Apolipoproteins E ,Settore BIO/13 - Biologia Applicata ,Alzheimer Disease ,genetics [Apolipoprotein E2] ,Humans ,BEHAVIORAL DEFICITS ,ddc:610 ,Age of Onset ,PROGRESS ,Alleles ,genetics [Apolipoprotein E4] ,Alzheimer's disease, genetics, APOE, risk factor ,A-BETA ,Original Investigation ,Settore MED/26 - NEUROLOGIA ,ALLELE ,genetics [Apolipoproteins E] ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Female ,INFERENCE ,Neurology (clinical) ,Human medicine - Abstract
34 páginas, 2 tablas, 2 figuras. 2 ficheros con material suplementario. Data used in preparation of this manuscript can be obtained upon application at: - dbGaP (https://www.ncbi.nlm.nih.gov/gap/advanced_search/) - NIAGADS and NIAGADS DSS (https://www.niagads.org/) - LONI (https://ida.loni.usc.edu/) - Synapse (https://adknowledgeportal.synapse.org/) - RADC Rush (https://www.radc.rush.edu/) - NACC (https://naccdata.org/) - UK Biobank (https://biobank.ndph.ox.ac.uk/showcase/), The APOE ε2 and APOE ε4 alleles are the strongest protective and risk-increasing, respectively, genetic variants for late-onset Alzheimer disease (AD). However, the mechanisms linking APOE to AD-particularly the apoE protein's role in AD pathogenesis and how this is affected by APOE variants-remain poorly understood. Identifying missense variants in addition to APOE ε2 and APOE ε4 could provide critical new insights, but given the low frequency of additional missense variants, AD genetic cohorts have previously been too small to interrogate this question robustly., This work was supported by the National Institute of Health and National Institute of Aging grants AG060747 (MDG), AG066206 (ZH), AG066515 (ZH, MDG), the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie (grant agreement No. 890650, YLG), the Alzheimer’s Association (AARF-20-683984, MEB), and the Iqbal Farrukh and Asad Jamal Fund, a grant from the EU Joint Programme – Neurodegenerative Disease Research (European Alzheimer DNA BioBank, EADB; JPND). Inserm UMR1167 is also funded by the Inserm, Institut Pasteur de Lille, Lille Métropole Communauté Urbaine, and the French government’s LABEX DISTALZ program (development of innovative strategies for a transdisciplinary approach to Alzheimer’s disease). EADB thank the study participants, researchers, and staff for collecting and contributing to the data, the high-performance computing service at the University of Lille, and the staff at CEA-CNRGH for their help with sample preparation and genotyping, and technical assistance. Additional funders of individual investigators and institutions who contributed to data collection and genotyping are provided in the Supplemental Online Content.
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- 2022
21. RESPONSE
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de Montalembert, M., Belloy, M., Elion, J., and Girot, R.
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- 1994
22. Protective function and differentiation cues of brain-resident CD8+ T cells during surveillance of latent Toxoplasma gondii infection.
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Porte R, Belloy M, Audibert A, Bassot E, Aïda A, Alis M, Miranda-Capet R, Jourdes A, van Gisbergen KPJM, Masson F, and Blanchard N
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- Animals, Mice, Latent Infection immunology, Latent Infection parasitology, Antigens, CD metabolism, Antigens, CD immunology, Antigens, CD genetics, Mice, Inbred C57BL, Female, CD8-Positive T-Lymphocytes immunology, Toxoplasma immunology, Brain immunology, Brain parasitology, Cell Differentiation immunology, Toxoplasmosis immunology, Toxoplasmosis parasitology
- Abstract
Chronic Toxoplasma gondii infection induces brain-resident CD8+ T cells (bTr), but the protective functions and differentiation cues of these cells remain undefined. Here, we used a mouse model of latent infection by T. gondii leading to effective CD8+ T cell-mediated parasite control. Thanks to antibody depletion approaches, we found that peripheral circulating CD8+ T cells are dispensable for brain parasite control during chronic stage, indicating that CD8+ bTr are able to prevent brain parasite reactivation. We observed that the retention markers CD69, CD49a, and CD103 are sequentially acquired by brain parasite-specific CD8+ T cells throughout infection and that a majority of CD69/CD49a/CD103 triple-positive (TP) CD8+ T cells also express Hobit, a transcription factor associated with tissue residency. This TP subset develops in a CD4+ T cell-dependent manner and is associated with effective parasite control during chronic stage. Conditional invalidation of Transporter associated with Antigen Processing (TAP)-mediated major histocompatibility complex (MHC) class I presentation showed that presentation of parasite antigens by glutamatergic neurons and microglia regulates the differentiation of CD8+ bTr into TP cells. Single-cell transcriptomic analyses revealed that resistance to encephalitis is associated with the expansion of stem-like subsets of CD8+ bTr. In summary, parasite-specific brain-resident CD8+ T cells are a functionally heterogeneous compartment which autonomously ensure parasite control during T. gondii latent infection and which differentiation is shaped by neuronal and microglial MHC I presentation. A more detailed understanding of local T cell-mediated immune surveillance of this common parasite is needed for harnessing brain-resident CD8+ T cells in order to enhance control of chronic brain infections., Competing Interests: Competing interests statement:The authors declare no competing interest.
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- 2024
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23. APOE loss-of-function variants: Compatible with longevity and associated with resistance to Alzheimer's Disease pathology.
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Chemparathy A, Guen YL, Chen S, Lee EG, Leong L, Gorzynski J, Xu G, Belloy M, Kasireddy N, Tauber AP, Williams K, Stewart I, Wingo T, Lah J, Jayadev S, Hales C, Peskind E, Child DD, Keene CD, Cong L, Ashley E, Yu CE, and Greicius MD
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The ε4 allele of apolipoprotein E ( APOE ) is the strongest genetic risk factor for sporadic Alzheimer's Disease (AD). Knockdown of this allele may provide a therapeutic strategy for AD, but the effect of APOE loss-of-function (LoF) on AD pathogenesis is unknown. We searched for APOE LoF variants in a large cohort of older controls and patients with AD and identified six heterozygote carriers of APOE LoF variants. Five carriers were controls (ages 71-90) and one was an AD case with an unremarkable age-at-onset between 75-79. Two APOE ε3/ε4 controls (Subjects 1 and 2) carried a stop-gain affecting the ε4 allele. Subject 1 was cognitively normal at 90+ and had no neuritic plaques at autopsy. Subject 2 was cognitively healthy within the age range 75-79 and underwent lumbar puncture at between ages 75-79 with normal levels of amyloid. The results provide the strongest human genetics evidence yet available suggesting that ε4 drives AD risk through a gain of abnormal function and support knockdown of APOE ε4 or its protein product as a viable therapeutic option., Competing Interests: Declaration of interests The authors declare no competing interests.
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- 2023
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24. Delayed haemolytic transfusion reaction in paediatric patients with sickle cell disease: A retrospective study in a French national reference centre.
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Rossi M, Pirenne F, Le Roux E, Smaïne D, Belloy M, Eyssette-Guerreau S, Couque N, Holvoet L, Ithier G, Brousse V, Koehl B, Faye A, Benkerrou M, and Missud F
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- Humans, Child, Retrospective Studies, Blood Transfusion, Anemia, Sickle Cell complications, Anemia, Sickle Cell therapy, Stroke prevention & control, Transfusion Reaction etiology
- Abstract
Delayed haemolytic transfusion reaction (DHTR) is a life-threatening haemolytic anaemia following red blood cell transfusion in patients with sickle cell disease, with only scarce data in children. We retrospectively analysed 41 cases of DHTR in children treated between 2006 and 2020 in a French university hospital. DHTR manifested at a median age of 10.5 years, symptoms occurred a median of 8 days after transfusion performed for an acute event (63%), before surgery (20%) or in a chronic transfusion programme (17%). In all, 93% of patients had painful crisis. Profound anaemia (median 49 g/L), low reticulocyte count (median 140 ×10
9 /L) and increased lactate dehydrogenase (median 2239 IU/L) were observed. Antibody screening was positive in 51% of patients, and more frequent when there was a history of alloimmunisation. Although no deaths were reported, significant complications occurred in 51% of patients: acute chest syndrome (12 patients), cholestasis (five patients), stroke (two patients) and kidney failure (two patients). A further transfusion was required in 23 patients and corticosteroids were used in 21 to reduce the risk of additional haemolysis. In all, 13 patients subsequently received further transfusions with recurrence of DHTR in only two. The study affords a better overview of DHTR and highlights the need to establish guidelines for its management in children., (© 2022 British Society for Haematology and John Wiley & Sons Ltd.)- Published
- 2023
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25. Delayed hemolytic transfusion reaction in children with sickle cell disease: first 5-year retrospective study in mainland France.
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Falguière C, Allali S, Khazem B, Kamdem A, Arnaud C, Belloy M, Guitton C, Odièvre MH, Pertuisel S, Dumesnil C, Guillaumat C, Garrec N, Gauthier A, Mahe P, Soussan-Banini V, Le-Carrer L, Merlin E, David A, Pellegrino B, Paillard C, Brasme JF, Lagarde M, Pirenne F, and Pondarre C
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- Child, Humans, Retrospective Studies, France epidemiology, Hemolysis, Isoantibodies, Anemia, Sickle Cell epidemiology, Anemia, Sickle Cell therapy, Transfusion Reaction epidemiology
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- 2023
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26. Varicella post-exposure management for pediatric oncology patients.
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Costa G, Orbach D, Saulpic J, Sarda-Thibault H, Hanslik T, Brethon B, Tabone MD, Raimbault S, Papillard S, Guillaumat C, Nathanson S, Pellegrino B, Belloy M, Mesples B, Trioche P, Jaber H, Raimondo G, Gilet C, and Cohen-Gogo S
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- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Male, Prospective Studies, Treatment Outcome, Chickenpox complications, Chickenpox prevention & control, Guideline Adherence statistics & numerical data, Neoplasms complications, Post-Exposure Prophylaxis standards, Practice Guidelines as Topic
- Abstract
Introduction: The objective was to evaluate health care providers' (HCP) adherence to and efficacy of varicella post-exposure prophylaxis (PEP) recommendations. It was an observational, prospective, multicenter study set in Ile-de-France, France., Methods: All children under 18 with a cancer diagnosis, currently or within 3months of receiving cancer treatment, regardless of varicella zoster virus (VZV) serostatus or previous personal history of varicella, were eligible. Study participants with significant exposure were reviewed prospectively for PEP indications. Main outcome measures were the percentage of exposure situations for which HCP were guideline-compliant, the proportion of available VZV serostatuses and the incidence of breakthrough varicella after different PEP approaches., Results: A total of 51 patients from 15 centers were enrolled after 52 exposure episodes. Median age at exposure was 5 years (range, 1-15). Exposure within the household led to 38% of episodes. Prophylactic treatment consisted in specific anti-VZV immunoglobulins (V-ZIG) (n=19) or in oral aciclovir (n=15). No prophylactic treatment was given for 18 patients (in compliance, n=16). In compliance with guidelines, 17 patients received V-ZIG, 11 did not develop varicella (65%, [95% CI, 39-90%]); 15 received aciclovir, 13 did not develop varicella (87%, [95% CI, 67-100%]). Breakthrough varicella occurred in 11 patients, with simple clinical course in all cases; in 8/47 (17%) episodes when PEP was guideline-compliant versus 3/5 (60%) when not., Discussion: Recommendations have been respected and are efficient. PEP needs to be standardized and a study carried out to define the optimal approach. Anti-VZV immunization of seronegative family members should be encouraged., (Copyright © 2022 Société Française du Cancer. Published by Elsevier Masson SAS. All rights reserved.)
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- 2022
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27. A Toxic Friend: Genotoxic and Mutagenic Activity of the Probiotic Strain Escherichia coli Nissle 1917.
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Nougayrède JP, Chagneau CV, Motta JP, Bossuet-Greif N, Belloy M, Taieb F, Gratadoux JJ, Thomas M, Langella P, and Oswald E
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- Animals, CHO Cells, Cricetulus, Escherichia coli Infections microbiology, Escherichia coli Proteins metabolism, Female, Genomic Islands, HeLa Cells, Humans, Mice, Mutation, DNA Damage, Epithelial Cells microbiology, Escherichia coli genetics, Escherichia coli Proteins genetics, Genome, Bacterial, Mutagenesis, Probiotics
- Abstract
The probiotic Escherichia coli strain Nissle 1917 (DSM 6601, Mutaflor), generally considered beneficial and safe, has been used for a century to treat various intestinal diseases. However, Nissle 1917 hosts in its genome the pks pathogenicity island that codes for the biosynthesis of the genotoxin colibactin. Colibactin is a potent DNA alkylator, suspected to play a role in colorectal cancer development. We show in this study that Nissle 1917 is functionally capable of producing colibactin and inducing interstrand cross-links in the genomic DNA of epithelial cells exposed to the probiotic. This toxicity was even exacerbated with lower doses of the probiotic, when the exposed cells started to divide again but exhibited aberrant anaphases and increased gene mutation frequency. DNA damage was confirmed in vivo in mouse models of intestinal colonization, demonstrating that Nissle 1917 produces the genotoxin in the gut lumen. Although it is possible that daily treatment of adult humans with their microbiota does not produce the same effects, administration of Nissle 1917 as a probiotic or as a chassis to deliver therapeutics might exert long-term adverse effects and thus should be considered in a risk-versus-benefit evaluation. IMPORTANCE Nissle 1917 is sold as a probiotic and considered safe even though it has been known since 2006 that it harbors the genes for colibactin synthesis. Colibactin is a potent genotoxin that is now linked to causative mutations found in human colorectal cancer. Many papers concerning the use of this strain in clinical applications ignore or elude this fact or misleadingly suggest that Nissle 1917 does not induce DNA damage. Here, we demonstrate that Nissle 1917 produces colibactin in vitro and in vivo and induces mutagenic DNA damage. This is a serious safety concern that must not be ignored in the interests of patients, the general public, health care professionals, and ethical probiotic manufacturers.
- Published
- 2021
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28. Evaluation of Outcomes and Quality of Care in Children with Sickle Cell Disease Diagnosed by Newborn Screening: A Real-World Nation-Wide Study in France.
- Author
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Brousse V, Arnaud C, Lesprit E, Quinet B, Odièvre MH, Etienne-Julan M, Guillaumat C, Elana G, Belloy M, Garnier N, Chamouine A, Dumesnil C, Montalembert M, Pondarre C, Bernaudin F, Couque N, Boutin E, Bardakjian J, Djennaoui F, Ithier G, Benkerrou M, and Thuret I
- Abstract
This study's objective was to assess, on a national scale, residual risks of death, major disease-related events, and quality of care during the first five years in children diagnosed at birth with sickle cell disease (SCD). Data were retrospectively collected from medical files of all children with SCD born between 2006-2010 in France. Out of 1792 eligible subjects, 1620 patients (71.8% SS or S/beta°-thalassemia -SB°-) had available follow-up data, across 69 centers. Overall probability of survival by five years was 98.9%, with 12/18 deaths related to SCD. Probability of overt stroke by five years in SS/SB° patients was 1.1%, while transcranial Doppler (TCD) was performed in 81% before three years of age. A total of 26 patients had meningitis/septicemia (pneumococcal in eight cases). Prophylactic penicillin was started at a median age of 2.2 months and 87% of children had received appropriate conjugate pneumococcal vaccination at one year. By five years, the probability of survival without SCD-related events was 10.7% for SS/SB° patients. In contrast, hydroxyurea was prescribed in 13.7% and bone marrow transplant performed in nine patients only. In this study, residual risks of severe complications were low, probably resulting from a good national TCD, vaccination, and healthcare system coverage. Nonetheless, burden of disease remained high, stressing the need for disease-modifying or curative therapy., Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
- Published
- 2019
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29. Bottom-up sensory processing can induce negative BOLD responses and reduce functional connectivity in nodes of the default mode-like network in rats.
- Author
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Hinz R, Peeters LM, Shah D, Missault S, Belloy M, Vanreusel V, Malekzadeh M, Verhoye M, Van der Linden A, and Keliris GA
- Subjects
- Animals, Brain Mapping, Magnetic Resonance Imaging, Male, Neural Pathways physiology, Photic Stimulation, Rats, Long-Evans, Attention physiology, Brain physiology, Visual Perception physiology
- Abstract
The default mode network is a large-scale brain network that is active during rest and internally focused states and deactivates as well as desynchronizes during externally oriented (top-down) attention demanding cognitive tasks. However, it is not sufficiently understood if salient stimuli, able to trigger bottom-up attentional processes, could also result in similar reduction of activity and functional connectivity in the DMN. In this study, we investigated whether bottom-up sensory processing could influence the default mode-like network (DMLN) in rats. DMLN activity was examined using block-design visual functional magnetic resonance imaging (fMRI) while its synchronization was investigated by comparing functional connectivity during a resting versus a continuously stimulated brain state by unpredicted light flashes. We demonstrated that the BOLD response in DMLN regions was decreased during visual stimulus blocks and increased during blanks. Furthermore, decreased inter-network functional connectivity between the DMLN and visual networks as well as decreased intra-network functional connectivity within the DMLN was observed during the continuous visual stimulation. These results suggest that triggering of bottom-up attention mechanisms in sedated rats can lead to a cascade similar to top-down orienting of attention in humans and is able to deactivate and desynchronize the DMLN., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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30. Robust Control of a Brain-Persisting Parasite through MHC I Presentation by Infected Neurons.
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Salvioni A, Belloy M, Lebourg A, Bassot E, Cantaloube-Ferrieu V, Vasseur V, Blanié S, Liblau RS, Suberbielle E, Robey EA, and Blanchard N
- Subjects
- Animals, Antigens, Protozoan immunology, Brain cytology, Brain parasitology, Cell Line, Cells, Cultured, Histocompatibility Antigens Class I genetics, Humans, Male, Mice, Mice, Inbred C57BL, Neurons immunology, Neurons parasitology, Protozoan Proteins immunology, Toxoplasma immunology, Toxoplasma pathogenicity, Antibodies, Protozoan immunology, Brain immunology, Histocompatibility Antigens Class I immunology, Toxoplasmosis, Cerebral immunology
- Abstract
Control of CNS pathogens by CD8 T cells is key to avoid fatal neuroinflammation. Yet, the modalities of MHC I presentation in the brain are poorly understood. Here, we analyze the antigen presentation mechanisms underlying CD8 T cell-mediated control of the Toxoplasma gondii parasite in the CNS. We show that MHC I presentation of an efficiently processed model antigen (GRA6-OVA), even when not expressed in the bradyzoite stage, reduces cyst burden and dampens encephalitis in C57BL/6 mice. Antigen presentation assays with infected primary neurons reveal a correlation between lower MHC I presentation of tachyzoite antigens by neurons and poor parasite control in vivo. Using conditional MHC I-deficient mice, we find that neuronal MHC I presentation is required for robust restriction of T. gondii in the CNS during chronic phase, showing the importance of MHC I presentation by CNS neurons in the control of a prevalent brain pathogen., (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
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31. Quasi-periodic patterns contribute to functional connectivity in the brain.
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Abbas A, Belloy M, Kashyap A, Billings J, Nezafati M, Schumacher EH, and Keilholz S
- Subjects
- Adult, Brain Mapping methods, Female, Humans, Image Processing, Computer-Assisted methods, Male, Young Adult, Brain physiology, Nerve Net physiology, Neural Pathways physiology
- Abstract
Functional connectivity is widely used to study the coordination of activity between brain regions over time. Functional connectivity in the default mode and task positive networks is particularly important for normal brain function. However, the processes that give rise to functional connectivity in the brain are not fully understood. It has been postulated that low-frequency neural activity plays a key role in establishing the functional architecture of the brain. Quasi-periodic patterns (QPPs) are a reliably observable form of low-frequency neural activity that involve the default mode and task positive networks. Here, QPPs from resting-state and working memory task-performing individuals were acquired. The spatiotemporal pattern, strength, and frequency of the QPPs between the two groups were compared and the contribution of QPPs to functional connectivity in the brain was measured. In task-performing individuals, the spatiotemporal pattern of the QPP changes, particularly in task-relevant regions, and the QPP tends to occur with greater strength and frequency. Differences in the QPPs between the two groups could partially account for the variance in functional connectivity between resting-state and task-performing individuals. The QPPs contribute strongly to connectivity in the default mode and task positive networks and to the strength of anti-correlation seen between the two networks. Many of the connections affected by QPPs are also disrupted during several neurological disorders. These findings contribute to understanding the dynamic neural processes that give rise to functional connectivity in the brain and how they may be disrupted during disease., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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32. Molecular Imaging of Immune Cell Dynamics During De- and Remyelination in the Cuprizone Model of Multiple Sclerosis by [ 18 F]DPA-714 PET and MRI.
- Author
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Zinnhardt B, Belloy M, Fricke IB, Orije J, Guglielmetti C, Hermann S, Wagner S, Schäfers M, Van der Linden A, and Jacobs AH
- Subjects
- Animals, Brain diagnostic imaging, Brain metabolism, Cuprizone toxicity, Female, Fluorine Radioisotopes, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL, Multiple Sclerosis etiology, Multiple Sclerosis immunology, Myelin Sheath metabolism, Multiple Sclerosis diagnostic imaging, Neuroglia immunology, Positron-Emission Tomography, Pyrazoles pharmacokinetics, Pyrimidines pharmacokinetics, Radiopharmaceuticals pharmacokinetics
- Abstract
Background : Activation and dysregulation of innate, adaptive and resident immune cells in response to damage determine the pathophysiology of demyelinating disorders. Among the plethora of involved cells, microglia/macrophages and astrocytes play an important role in the pathogenesis of demyelinating disorders. The in-depth investigation of the spatio-temporal profile of these cell types in vivo may inform about the exact disease state and localization as well as may allow to monitor therapeutic modulation of the components of the neuroinflammatory response during the course of multiple sclerosis (MS). In this study, we aimed to non-invasively decipher the degree and temporal profile of neuroinflammation (TSPO - [
18 F]DPA-714 PET) in relation to selected magnetic resonance imaging (MRI) parameters (T2 maps) in the cuprizone (CPZ)-induced model of demyelination. Methods: C57Bl6 ( n=30 ) mice were fed with a standard chow mixed with 0.2% (w/w) CPZ for 4 ( n=10 ; demyelination) and 6 weeks ( n=10 ; spontaneous remyelination). The degree of neuroinflammation at de- and remyelination was assessed by [18 F]DPA-714 PET, multi-echo T2 MRI, autoradiography and immunohistochemistry. Results : CPZ-induced brain alterations were confirmed by increase of T2 relaxation times in both white and grey matter after 3 and 5 weeks of CPZ. Peak [18 F]DPA-714 was found in the corpus callosum (CC, white matter), the hippocampus (HC, grey matter) and thalamus (grey matter) after 4 weeks of CPZ treatment and declined after 6 weeks of CPZ. Ex vivo autoradiography and dedicated immunofluorescence showed demyelination/remyelination with corresponding increased/decreased TSPO levels in the CC and hippocampus, confirming the spatial distribution of [18 F]DPA-714 in vivo . The expression of TSPO microglia and astrocytes is time-dependent in this model. Microglia predominantly express TSPO at demyelination, while the majority of astrocytes express TSPO during remyelination. The combination of PET- and MRI-based imaging biomarkers demonstrated the regional and temporal development of the CPZ model-associated neuroinflammatory response in grey and white matter regions. Conclusions : The combination of [18 F]DPA-714 PET and T2 mapping may allow to further elucidate the regional and temporal profile of inflammatory signals depending on the myelination status, although the underlying inflammatory microenvironment changes. A combination of the described imaging biomarkers may facilitate the development of patient-tailored strategies for immunomodulatory and neuro-restorative therapies in MS., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.- Published
- 2019
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33. Protocol for HeLa Cells Infection with Escherichia coli Strains Producing Colibactin and Quantification of the Induced DNA-damage.
- Author
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Bossuet-Greif N, Belloy M, Boury M, Oswald E, and Nougayrede JP
- Abstract
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death. Here we describe a simple method to demonstrate the genotoxicity of bacteria producing colibactin following a short infection of cultured mammalian cells with pks
+ E. coli ., (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.)- Published
- 2017
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34. [Care pathways before diagnosis in children and adolescents with malignancies].
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Tatencloux S, Mosseri V, Papillard-Maréchal S, Mesples B, Pellegrino B, Belloy M, Jimènez I, Algret N, Levy D, Michon J, and Orbach D
- Subjects
- Adolescent, Bone Neoplasms complications, Bone Neoplasms diagnosis, Bone Neoplasms epidemiology, Brain Neoplasms complications, Brain Neoplasms diagnosis, Brain Neoplasms epidemiology, Cancer Care Facilities, Cancer Pain etiology, Child, Child, Preschool, Delayed Diagnosis statistics & numerical data, Emigrants and Immigrants statistics & numerical data, Family Characteristics, Female, France, General Practice statistics & numerical data, Humans, Infant, Infant, Newborn, Male, Mesenchymoma complications, Mesenchymoma diagnosis, Mesenchymoma epidemiology, Neoplasms complications, Neoplasms epidemiology, Retrospective Studies, Sarcoma diagnosis, Sarcoma epidemiology, Socioeconomic Factors, Symptom Assessment, Young Adult, Delayed Diagnosis adverse effects, Neoplasms diagnosis
- Abstract
Objective: to describe medical care pathways between first symptoms and first oncologic consultation in children and adolescents with solid cancers in order to analyze a possible relationship between delayed diagnosis and its potential consequences., Methods: Retrospective study on patients aged less than 25 years at first consultation in the oncology department of pediatric, adolescent and young adult in Institut Curie during one year. Were collected data on cancer characteristics, components of care pathways, and sociodemographic parents' characteristics., Results: Hundred and six patients were selected, with median age of 6 years. Most represented tumor was low-grade cerebral tumor (17.0%). Pain was the most frequent type of disorder observed as first sign (34.3% of patients). First signs were unspecific in only 27.6% of cases. Most patients were first seen by a general practitioner (29.3%). Median total time to diagnosis was one month [ranges: 0-64]. Median number of consultations before referral to oncology expert was 2 [0-7]. Retrospective analysis found a possible delayed diagnosis in 44.3% of patients, with potential vital and functional risks estimated respectively at 14.1 and 20.7% of overall population. Time to diagnosis was shorter if father was of foreign nationality vs. French (34 days vs. 72 days, P<0.05), and longer if parents were separated (74.5 days vs. 42.5 days, P<0.03)., Conclusions: Overall time to diagnosis is quite fast, even if first signs of pediatric cancers are very polymorphic. Some medical and sociodemographic factors could influence characteristics of care pathways., (Copyright © 2016 Société Française du Cancer. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2017
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35. Improvement of medical care in a cohort of newborns with sickle-cell disease in North Paris: impact of national guidelines.
- Author
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Couque N, Girard D, Ducrocq R, Boizeau P, Haouari Z, Missud F, Holvoet L, Ithier G, Belloy M, Odièvre MH, Benemou M, Benhaim P, Retali B, Bensaid P, Monier B, Brousse V, Amira R, Orzechowski C, Lesprit E, Mangyanda L, Garrec N, Elion J, Alberti C, Baruchel A, and Benkerrou M
- Subjects
- Acute Chest Syndrome etiology, Child, Child, Preschool, Cohort Studies, Female, Follow-Up Studies, Humans, Hydroxyurea therapeutic use, Infant, Newborn, Male, Paris, Retrospective Studies, Stroke etiology, Thalassemia, Anemia, Sickle Cell complications, Anemia, Sickle Cell drug therapy, Anemia, Sickle Cell mortality, Guideline Adherence, Quality Improvement standards
- Abstract
We conducted a retrospective study on newborns with sickle-cell disease (SCD), born 1995-2009, followed in a multicentre hospital-based network. We assessed patient outcomes, medical care and compliance with the national guidelines published in December 2005. Data from 1033 patients (742 SS/Sβ°-thalassaemia) with 6776 patient-years of follow-up were analysed (mean age 7·1 ± 3·9 years). SCD-related deaths (n = 13) occurred only in SS-genotype patients at a median age of 23·1 months, mainly due to acute anaemia (n = 5, including 2 acute splenic sequestrations) and infection (n = 3). Treatment non-compliance was associated with a 10-fold higher risk of SCD-related death (P = 0·01). Therapeutic intensification was provided for all stroke patients (n = 12), almost all patients with abnormal transcranial Doppler (TCD) (n = 76) or with >1 acute chest syndrome/lifetime (n = 64) and/or ≥3 severe vaso-occlusive crises/year (n = 100). Only 2/3 of patients with baseline haemoglobin <70 g/l received intensification, mainly for other severity criteria. Overall, hydroxycarbamide was under-prescribed, given to 2/3 of severe vaso-occlusive patients and 1/3 of severely anaemic patients. Nevertheless, introduction of the on-line guidelines was concomitant with an improvement in medical care in the 2006-2009 cohort with a trend towards increased survival at 5 years, from 98·3% to 99·2%, significantly increased TCD coverage (P = 0·004) and earlier initiation of intensification of therapy (P ≤ 0·01)., (© 2016 John Wiley & Sons Ltd.)
- Published
- 2016
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36. Fatal transfusion-transmitted infection due to Citrobacter koseri.
- Author
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Hauser L, Menasie S, Bonacorsi S, Raoult L, AitOubelli N, Belloy M, Avran D, Beyloune A, Simonet M, Billard-Pomares T, Pangon B, and Bierling P
- Abstract
Background: Transfusion-transmitted bacterial infection (TTBI) is still one of the most feared complications of blood transfusion., Case Report: We report a fatal case involving an 8-year-old child with congenital dyskeratosis complicated by severe aplastic anemia who was regularly transfused with platelet (PLT) concentrates for 5 years. The patient received an apheresis PLT concentrate (APC) on Day 0 due to thrombocytopenia complicated by mucocutaneous hemorrhage. Thirty minutes after the start of the transfusion, bradycardia and dyspnea appeared, quickly followed by chills, nausea, vomiting, headache, and hyperthermia. TTBI was suspected and the patient was immediately treated with intravascular antibiotherapy. On Day 3, the patient developed severe acute respiratory distress syndrome leading to death on Day 7. Patient blood cultures and APC cultures were both positive for Citrobacter koseri., Results: The donor was a 19-year-old woman. She had previously given blood. No infectious symptom was reported during the medical interviews before and after the donation and no postdonation information was received. On the day of the donation (Day -2), her white blood cell count was 5.83 × 10
9 /L. She came back on Day 8 to undergo additional tests. The cultures from blood, stool, urine, the skin of the inside of the elbow at the point of needle insertion, and ear samples were all negative for C. koseri. However, a nasal sample was positive for C. koseri., Conclusion: The isolates from the donor's blood cultures, the APC bag, the attached tube, and the donor's nasal sample all gave identical profiles; they were thus identified as the same strain and the TTBI was confirmed., (© 2016 AABB.)- Published
- 2016
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37. Shear Stress-Induced Alteration of Epithelial Organization in Human Renal Tubular Cells.
- Author
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Maggiorani D, Dissard R, Belloy M, Saulnier-Blache JS, Casemayou A, Ducasse L, Grès S, Bellière J, Caubet C, Bascands JL, Schanstra JP, and Buffin-Meyer B
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Animals, Cadherins metabolism, Claudin-2 metabolism, Epithelial Cells metabolism, Humans, Kidney metabolism, Kidney Tubules metabolism, Mice, Tubulin metabolism, Zonula Occludens-1 Protein metabolism, beta Catenin metabolism, Cilia metabolism, Epithelial Cells cytology, Kidney cytology, Kidney Tubules cytology, Stress, Mechanical, Tight Junctions metabolism
- Abstract
Tubular epithelial cells in the kidney are continuously exposed to urinary fluid shear stress (FSS) generated by urine movement and recent in vitro studies suggest that changes of FSS could contribute to kidney injury. However it is unclear whether FSS alters the epithelial characteristics of the renal tubule. Here, we evaluated in vitro and in vivo the influence of FSS on epithelial characteristics of renal proximal tubular cells taking the organization of junctional complexes and the presence of the primary cilium as markers of epithelial phenotype. Human tubular cells (HK-2) were subjected to FSS (0.5 Pa) for 48 h. Control cells were maintained under static conditions. Markers of tight junctions (Claudin-2, ZO-1), Par polarity complex (Pard6), adherens junctions (E-Cadherin, β-Catenin) and the primary cilium (α-acetylated Tubulin) were analysed by quantitative PCR, Western blot or immunocytochemistry. In response to FSS, Claudin-2 disappeared and ZO-1 displayed punctuated and discontinuous staining in the plasma membrane. Expression of Pard6 was also decreased. Moreover, E-Cadherin abundance was decreased, while its major repressors Snail1 and Snail2 were overexpressed, and β-Catenin staining was disrupted along the cell periphery. Finally, FSS subjected-cells exhibited disappeared primary cilium. Results were confirmed in vivo in a uninephrectomy (8 months) mouse model where increased FSS induced by adaptive hyperfiltration in remnant kidney was accompanied by both decreased epithelial gene expression including ZO-1, E-cadherin and β-Catenin and disappearance of tubular cilia. In conclusion, these results show that proximal tubular cells lose an important number of their epithelial characteristics after long term exposure to FSS both in vitro and in vivo. Thus, the changes in urinary FSS associated with nephropathies should be considered as potential insults for tubular cells leading to disorganization of the tubular epithelium.
- Published
- 2015
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38. Temporal dynamics of interferon gamma responses in children evaluated for tuberculosis.
- Author
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Herrmann JL, Belloy M, Porcher R, Simonney N, Aboutaam R, Lebourgeois M, Gaudelus J, De Losangeles L, Chadelat K, Scheinmann P, Beydon N, Fauroux B, Bingen M, Terki M, Barraud D, Cruaud P, Offredo C, Ferroni A, Berche P, Moissenet D, Vuthien H, Doit C, Bingen E, and Lagrange PH
- Subjects
- Adolescent, Adult, BCG Vaccine immunology, Child, Child, Preschool, Female, France epidemiology, Humans, Infant, Infant, Newborn, Male, Prospective Studies, ROC Curve, Reagent Kits, Diagnostic, Time Factors, Tuberculosis epidemiology, Tuberculosis prevention & control, Interferon-gamma immunology, Tuberculosis diagnosis, Tuberculosis immunology
- Abstract
Background: Development of T-cells based-Interferon gamma (IFNgamma) assays has offered new possibilities for the diagnosis of latent tuberculosis infection (LTBI) and active disease in adults. Few studies have been performed in children, none in France. With reference to the published data on childhood TB epidemiology in the Paris and Ile de France Region, we considered it important to evaluate the performance of IGRA (QuantiFERON TB Gold In Tube(R), QF-TB-IT) in the diagnosis and the follow-up through treatment of LTBI and active TB in a cohort of French children., Methodology/principal Findings: 131 children were recruited during a prospective and multicentre study (October 2005 and May 2007; Ethical Committee St Louis Hospital, Paris, study number 2005/32). Children were sampled at day 0, 10, 30, 60 (except Healthy Contacts, HC) and 90 for LTBI and HC, and a further day 120, and day 180 for active TB children. Median age was 7.4 years, with 91% of the children BCG vaccinated. LTBI and active TB children undergoing therapy produced significant higher IFNgamma values after 10 days of treatment (p = 0.035). In addition, IFNgamma values were significantly lower at the end of treatment compared to IFNgamma values at day 0, although the number of positive patients was not significantly different between day 0 and end of treatment., Conclusions/ Significance: By following quantitative IFNgamma values in each enrolled child with LTBI or active TB and receiving treatment, we were able to detect an increase in the IFNgamma response at day 10 of treatment which might allow the confirmation of a diagnosis. In addition, a decline in IFNgamma values during treatment makes it possible for clinicians to monitor the effect of preventive or curative therapy.
- Published
- 2009
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39. [Kawasaki disease epidemiology in Guadeloupe].
- Author
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Tourneux P, Dufillot D, Belloy M, Boralevi F, Cevallos L, and Krim G
- Subjects
- Age Distribution, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Aspirin therapeutic use, Child, Child, Preschool, Echocardiography, Female, Fever etiology, Guadeloupe epidemiology, Humans, Immunoglobulins, Intravenous therapeutic use, Incidence, Infant, Leukocytosis etiology, Male, Mucocutaneous Lymph Node Syndrome diagnosis, Mucocutaneous Lymph Node Syndrome drug therapy, Mucocutaneous Lymph Node Syndrome etiology, Patient Selection, Population Surveillance, Retrospective Studies, Risk Factors, Seasons, Sex Distribution, Thrombocytosis etiology, Treatment Outcome, Mucocutaneous Lymph Node Syndrome epidemiology
- Abstract
Objective: To study the incidence of Kawasaki disease in the population of the French West Indies., Methods: Fifty-six children where enrolled between January 1, 1995 and December 31, 2000), in this retrospective study in Guadeloupe (French West Indies), according to the diagnostic criteria of the American Heart Association., Results: There were 31 boys and 25 girls. Their mean age at the time of diagnosis was 26.5 +/- 22 months. The mean incidence was 25.4/100 000 children aged under 5, per year. Cardiac involvement was noted in 17.8% of the cases during the first 3 months, with good outcome in all the children followed-up., Conclusion: The incidence of Kawasaki disease in Guadeloupe is high. The absence of epidemiological, clinical or biological predictive criteria of cardiac involvement should prompt the early use of immunoglobulins, notably in atypical presentations of the disease.
- Published
- 2005
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40. [Kawasaki disease and dengue: a fortuitous association?].
- Author
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Tourneux P, Dufillot D, Boussemart T, and Belloy M
- Subjects
- Age Factors, Child, Preschool, Dengue diagnosis, Female, Humans, Infant, Male, Mucocutaneous Lymph Node Syndrome diagnosis, Retrospective Studies, Sex Factors, Dengue complications, Mucocutaneous Lymph Node Syndrome complications
- Published
- 2002
- Full Text
- View/download PDF
41. [Neonatal screening for sickle cell anemia: evaluation of a five-year experience in an area of northern Paris].
- Author
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Ducrocq R, Benkerrou M, Brahimi L, Belloy M, Briard ML, Vilmer E, and Elion J
- Subjects
- Anemia, Sickle Cell epidemiology, False Positive Reactions, Female, France epidemiology, Humans, Incidence, Infant, Newborn, Male, Urban Population, Anemia, Sickle Cell diagnosis, Mass Screening
- Abstract
Unlabelled: We report a five-year experience of targeted neonatal screening for sickle cell disease in the northern part of the Paris area as well as the follow-up procedure of screened patients., Population: This geographic area in France is characterized by a high frequency of populations at risk for sickle cell disease., Results: Among 115,480 tested newborns, 250 patients were diagnosed (frequency: 1/462). The quality of the screening was attested by the high frequency (5.34%) of newborn carriers for a hemoglobin abnormality (n = 6168). We developed an optimized strategy which avoids the majority of pitfalls (false positive and false negative responses), except for S/HPFH. More than 95% of sickle cell disease was followed, the majority by medical sickle cell disease experts from hospitals. Only two deaths were recorded during this time period., Conclusion: We demonstrate the efficiency of targeting the proposed methodological strategy and the follow-up of affected newborns, a major argument demonstrating the importance of newborn screening.
- Published
- 2001
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42. Acute clinical events in 299 homozygous sickle cell patients living in France. French Study Group on Sickle Cell Disease.
- Author
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Neonato MG, Guilloud-Bataille M, Beauvais P, Bégué P, Belloy M, Benkerrou M, Ducrocq R, Maier-Redelsperger M, de Montalembert M, Quinet B, Elion J, Feingold J, and Girot R
- Subjects
- Acute Disease, Adolescent, Anemia, Sickle Cell genetics, Child, Child, Preschool, Cohort Studies, Female, France epidemiology, Genotype, Humans, Male, Meningitis complications, Meningitis epidemiology, Osteomyelitis complications, Osteomyelitis epidemiology, Phenotype, Risk Factors, Sepsis complications, Sepsis epidemiology, Stroke complications, Stroke epidemiology, alpha-Thalassemia complications, alpha-Thalassemia genetics, Anemia, Sickle Cell complications
- Abstract
A subset of 299 patients with homozygous sickle cell anaemia, enrolled in the cohort of the French Study Group on sickle cell disease (SCD), was investigated in this study. The majority of patients were children (mean age 10.1 +/- 5.8 yr) of first generation immigrants from Western and Central Africa, the others originated from the French West Indies (20.2%). We report the frequency of the main clinical events (mean follow-up 4.2 +/- 2.2 yr). The prevalence of meningitis-septicaemia and osteomyelitis was, respectively, 11.4% and 12% acute chest syndrome was observed in 134 patients (44.8%). Twenty patients (6.7%) developed stroke with peak prevalence at 10-15 yr of age. One hundred and seventy-two patients (58%) suffered from one or more painful sickle cell crises, while the others (42.5%) never suffered from pain. The overall frequency of acute anaemic episodes was 50.5%, (acute aplastic anaemia 46%; acute splenic sequestration 26%). A group of 27 patients were asymptomatic (follow-up > 3 yr). Epistatic mechanisms influencing SCD were studied. Coinherited alpha-thalassemia strongly reduced the risk of stroke (p <0.001) and increased that of painful crises (p < 0.02). There was a low prevalence of Senegal and Bantu (CAR) betas-chromosomes in patients with meningitis (p <0.04) and osteomyelitis (p < 0.03). Prevalence of Senegal betas-chromosomes was lower in the asymptomatic group of 27 patients (p < 0.02). The patients come from a population of unmixed immigrants in whom the beta-globin gene haplotype strongly reflects the geographic origin and identifies subgroups with a homogenous genetic background. Thus the observed effects might result more from differences in as yet unidentified determinants in the genetic background than from the direct linkage with differences in the beta-globin gene locus.
- Published
- 2000
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43. The relative importance of the X-linked FCP locus and beta-globin haplotypes in determining haemoglobin F levels: a study of SS patients homozygous for beta S haplotypes.
- Author
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Chang YP, Maier-Redelsperger M, Smith KD, Contu L, Ducroco R, de Montalembert M, Belloy M, Elion J, Dover GJ, and Girot R
- Subjects
- Adolescent, Adult, Anemia, Sickle Cell blood, Child, Child, Preschool, Female, Genetic Linkage, Haplotypes, Humans, Infant, Male, Phenotype, Regression Analysis, Reticulocytes pathology, X Chromosome, Anemia, Sickle Cell genetics, Fetal Hemoglobin analysis, Globins genetics
- Abstract
Five factors have been hypothesized to influence the 20-fold variation in fetal haemoglobin (Hb F) levels in sickle cell anaemia (SS): age sex, alpha-globin gene number, beta-globin haplotype, and the X-linked F-cell production locus (FCP) that regulates the production of Hb F containing erythrocytes (F cells). We analysed the association of these factors with Hb F levels in 112 SS patients living in France who are homozygous for the three common African beta-globin haplotypes (Benin, Bantu or Central African Republic and Senegal). We found that: (1) FCP accounts for about 40% of the overall variation in Hb F levels, (2) when the FCP influence is removed, beta-globin haplotype is associated with 14% of the remaining Hb F variation, and (3) the other factors have little influence. Comparison with our previous study of SS individuals in Jamaica leads to the following conclusions: (1) the X-linked FCP locus is a major determinant of Hb F levels in SS disease, (2) factors linked to the beta-globin haplotype have only a small effect on the variation in Hb F levels, in either the homozygous or heterozygous state, and (3)approximately half of the variation in Hb F levels still remains to be explained.
- Published
- 1997
- Full Text
- View/download PDF
44. [Prevention and treatment of erectile disorders in sickle cell disease].
- Author
-
Bachir D, Virag R, Lee K, Belloy M, de Montalembert M, Denis L, Broyart A, Girot R, and Galactéros F
- Subjects
- Adolescent, Adrenergic alpha-Agonists administration & dosage, Adrenergic alpha-Agonists therapeutic use, Adult, Child, Child, Preschool, Etilefrine administration & dosage, Etilefrine therapeutic use, Humans, Male, Priapism physiopathology, Priapism prevention & control, Time Factors, Priapism therapy, Sickle Cell Trait complications
- Abstract
Priapism is a frequent and serious cause of morbidity in males with sickle cell disease. Acute priapism (AP) is preceeded in two-thirds of the cases by repeated minor events called stuttering priapism (SP). Since 1994, we have used a specific approach to prevent the commonly devastating effects of AP, using the alpha adrenergic agent etilefrine. Treatment of AP has been simplified (drainage without aspiration followed by one or two intracavernous injections (ICI) of 10 mg of etilefrine, until detumescence). For SP lasting more than one hour or causing pain, we use oral etilefrine and/or self ICI. This strategy was effective in five patients seen having AP, 21 patients with SP; it is simple, cheap, and avoids surgical procedure and transfusion. Moreover, erectile dysfunction, present in three patients, has been treated safely by ICI of protaglandins.
- Published
- 1997
- Full Text
- View/download PDF
45. [Thalassemias and sickle cell anemias].
- Author
-
Belloy M
- Subjects
- France epidemiology, Humans, Anemia, Sickle Cell diagnosis, Anemia, Sickle Cell epidemiology, Anemia, Sickle Cell therapy, Emigration and Immigration, Thalassemia diagnosis, Thalassemia epidemiology, Thalassemia therapy
- Published
- 1992
46. [Sickle cell disease].
- Author
-
Belloy M
- Subjects
- Anemia, Sickle Cell physiopathology, Anemia, Sickle Cell therapy, Humans, Parents education, Anemia, Sickle Cell complications
- Published
- 1991
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