1. Detection of streptomycin residues in whole milk using an optical immunobiosensor
- Author
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Christopher T. Elliott, Ferguson Jp, Baxter Ga, and Mary C. O’Connor
- Subjects
Detection limit ,Residue (complex analysis) ,Maximum Residue Limit ,Chromatography ,Coefficient of variation ,General Chemistry ,Biosensing Techniques ,Sensitivity and Specificity ,Defatting ,Drug Residues ,Anti-Bacterial Agents ,chemistry.chemical_compound ,Milk ,chemistry ,Streptomycin ,medicine ,Animals ,General Agricultural and Biological Sciences ,Dihydrostreptomycin ,medicine.drug ,Antibacterial agent - Abstract
The development of an assay for the detection of streptomycin residues in pasteurized whole milk using an optical biosensor (Biacore) is reported. Streptomycin−adipic hydrazide coupled to bovine thyroglobulin was used to produce a sheep polyclonal antibody. The antibody displayed excellent cross-reactivity with dihydrostreptomycin (106%). There was no significant cross-reaction with other aminoglycosides or common antibiotics. Streptomycin was also immobilized onto a CM5 sensor chip to provide a stable, reusable surface. The developed assay permitted the direct analysis of whole milk samples (∼3.5% fat) without prior centrifugation and defatting. Results were available in 5 min. The limit of detection of the assay was determined as 4.1 ng/mL, well below the European maximum residue limit (MRL) of 200 ng/mL. Repeatability (or coefficient of variation) between runs was determined as 3.5% (100 ng/mL; 0.5 × MRL), 5.7% (200 ng/mL; MRL), and 7.6% (400 ng/mL; 2 × MRL). Keywords: Biosensor; streptomycin; drug res...
- Published
- 2001