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Determination of pantothenic acid in foods by optical biosensor immunoassay.
- Source :
-
Journal of AOAC International [J AOAC Int] 2005 Jul-Aug; Vol. 88 (4), pp. 1008-14. - Publication Year :
- 2005
-
Abstract
- An optical biosensor inhibition immunoassay was developed using a specific pantothenic acid-binding protein for the quantitation of free pantothenic acid (vitamin B5) in foodstuffs. Samples were prepared by a simple extraction procedure in buffer, and vitamin content was estimated against authentic calibrants in the same buffer. Performance parameters included a working range of 10-5000 ng/mL, a limit of detection of 4.4 ng/mL, precision relative standard deviation of 5.4-7.1% over a range of concentrations, and recoveries > 95% in the matrixes tested. A wide range of foodstuffs, including National Institute of Standards and Technology reference samples, were tested in 3 independent laboratories and the results were compared with microbiological assay and liquid chromatography/mass spectrometry (LC/MS) methods. The results indicate that the biosensor technique is appropriate for the estimation of pantothenic acid in a wide range of foodstuffs.
- Subjects :
- Biosensing Techniques instrumentation
Calibration
Chromatography, Liquid methods
Dose-Response Relationship, Drug
Food Analysis
Hydrogen-Ion Concentration
Immunoassay instrumentation
Infant Formula chemistry
Mass Spectrometry methods
Surface Plasmon Resonance
Biosensing Techniques methods
Dietary Supplements analysis
Immunoassay methods
Pantothenic Acid analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1060-3271
- Volume :
- 88
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of AOAC International
- Publication Type :
- Academic Journal
- Accession number :
- 16152915