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Feathers originate as protofeathers before birds, in pterosaurs and basal dinosaurs. What characterizes a feather is not only its outgrowth, but its barb cells differentiation and a set of beta-corneous proteins. Reticula appear concomitantly with feathers, as small bumps on plantar skin, made only of keratins. Avian scales, with their own set of beta-corneous proteins, appear more recently than feathers on the shank, and only in some species. In the chick embryo, when feather placodes form, all the non-feather areas of the integument are already specified. Among them, midventral apterium, cornea, reticula, and scale morphogenesis appear to be driven by negative regulatory mechanisms, which modulate the inherited capacity of the avian ectoderm to form feathers. Successive dermal/epidermal interactions, initiated by the Wnt/β-catenin pathway, and involving principally Eda/Edar , BMP, FGF 20 and Shh signaling, are responsible for the formation not only of feather, but also of scale placodes and reticula, with notable differences in the level of Shh, and probably FGF20 expressions. This sequence is a dynamic and labile process, the turning point being the FGF20 expression by the placode. This epidermal signal endows its associated dermis with the memory to aggregate and to stimulate the morphogenesis that follows, involving even a re-initiation of the placode. • Feather formation occurs by negative feedback mechanisms in non-feather areas. • FGF20 signal triggers epigenetic modifications of dermal fibroblasts potency. • Reticula are not scales and could not be considered as cutaneous appendages. • The first stage of feather evolution is suggested to correspond to a single barb. [ABSTRACT FROM AUTHOR]

Stingray envenomation is a common presenting complaint for coastal emergency departments in the United States. Currently, radiograph is the gold standard to evaluate for a retained stingray barb, but ultrasound may be a useful tool to detect retained barbs. To determine if emergency medicine residents could use ultrasound to identify stingray barbs embedded in animal tissue models. A secondary objective was to determine if resident experience affected their ability to detect stingray barbs. Thirty-two emergency medicine residents participated in the study. After a short didactic session on foreign body identification with ultrasound, they rotated through six simulation stations and were asked to identify whether a stingray barb was present in pig and chicken tissue models. They were given 2 min per model to identify the presence, size, and depth of a stingray barb. Pre- and postexperiment surveys were collected to assess the residents' level of experience and confidence regarding foreign body identification using ultrasound. Residents accurately identified barbs in chicken drumsticks with a sensitivity of 72.92% (95% confidence interval [CI] 63.89–81.48) and a specificity of 64.58% (95% CI 54.16–74.08), and in pig's feet with a sensitivity of 50.00% (95% CI 39.62–60.38) and specificity of 68.75% (95% CI 58.48–77.82). There was no statistically significant difference regarding accuracy for any outcome measured based on experience or level of training. The use of point-of-care ultrasound by novice sonographers lacks sensitivity to identify retained stingray barbs in animal models and is not significantly impacted by resident experience with point-of-care ultrasound. [ABSTRACT FROM AUTHOR]

Catfish injuries to the upper extremity following fishing activities are common in the southern United States, especially because noodling is commonplace in this region. Noodling is when a fisher will stick their hand into an area where a catfish is guarding its eggs and grab the catfish by its mouth. Different mechanisms of injury, including envenomation and spine embedment, can occur and ultimately lead to different patient presentations, including the retention of foreign bodies or infection. Literature reviews of catfish injuries primarily report the retention of foreign bodies within soft tissues, infection, and envenomation. We present the first case report of a patient who sustained a ring finger proximal phalangeal physeal injury involving the growth cartilage caused by a penetration injury from a catfish barb. A novel method for safely extracting these barbs with no subsequent growth arrest or range of motion limitation is also presented.

Simple Summary: Bred over centuries in the Maghreb region, on a corridor between the Arab and the Western world, the North African Barb horse has been touched by many influences in the course of history. The present study investigated the paternally inherited Y chromosome in today´s Barbs and Arab-Barbs collected from North Africa and Europe, with the aim to link genetic patterns and narrative history. A broad Y chromosomal spectrum was observed, as well as regional disparities among populations. Y chromosomal patterns illustrated a tight connection of Barb horses with Arabians and several other breeds, including Thoroughbreds. Besides, results depict footprints of past migrations between North Africa and the Iberian Peninsula. In horses, demographic patterns are complex due to historical migrations and eventful breeding histories. Particularly puzzling is the ancestry of the North African horse, a founding horse breed, shaped by numerous influences throughout history. A genetic marker particularly suitable to investigate the paternal demographic history of populations is the non-recombining male-specific region of the Y chromosome (MSY). Using a recently established horse MSY haplotype (HT) topology and KASP™ genotyping, we illustrate MSY HT spectra of 119 Barb and Arab-Barb males, collected from the Maghreb region and European subpopulations. All detected HTs belonged to the Crown haplogroup, and the broad MSY spectrum reflects the wide variety of influential stallions throughout the breed's history. Distinct HTs and regional disparities were characterized and a remarkable number of early introduced lineages were observed. The data indicate recent refinement with Thoroughbred and Arabian patrilines, while 57% of the dataset supports historical migrations between North Africa and the Iberian Peninsula. In the Barb horse, we detected the HT linked to Godolphin Arabian, one of the Thoroughbred founders. Hence, we shed new light on the question of the ancestry of one Thoroughbred patriline. We show the strength of the horse Y chromosome as a genealogical tool, enlighten recent paternal history of North African horses, and set the foundation for future studies on the breed and the formation of conservation breeding programs. [ABSTRACT FROM AUTHOR]

Abstract Background Sri Lanka is a continental island separated from India by the Palk Strait, a shallow-shelf sea, which was emergent during periods of lowered sea level. Its biodiversity is concentrated in its perhumid south-western ‘wet zone’. The island’s freshwater fishes are dominated by the Cyprinidae, characterized by small diversifications of species derived from dispersals from India. These include five diminutive, endemic species of Pethia (P. bandula, P. cumingii, P. melanomaculata, P. nigrofasciata, P. reval), whose evolutionary history remains poorly understood. Here, based on comprehensive geographic sampling, we explore the phylogeny, phylogeography and morphological diversity of the genus in Sri Lanka. Results The phylogenetic analyses, based on mitochondrial and nuclear loci, recover Sri Lankan Pethia as polyphyletic. The reciprocal monophyly of P. bandula and P. nigrofasciata, and P. cumingii and P. reval, is not supported. Pethia nigrofasciata, P. cumingii, and P. reval show strong phylogeographic structure in the wet zone, compared with P. melanomaculata, which ranges across the dry and intermediate zones. Translocated populations of P. nigrofasciata and P. reval in the Central Hills likely originate from multiple sources. Morphological analyses reveal populations of P. nigrofasciata proximal to P. bandula, a narrow-range endemic, to have a mix of characters between the two species. Similarly, populations of P. cumingii in the Kalu basin possess orange fins, a state between the red-finned P. reval from Kelani to Deduru and yellow-finned P. cumingii from Bentara to Gin basins. Conclusions Polyphyly in Sri Lankan Pethia suggests two or three colonizations from mainland India. Strong phylogeographic structure in P. nigrofasciata, P. cumingii and P. reval, compared with P. melanomaculata, supports a model wherein the topographically complex wet zone harbors greater genetic diversity than the topographically uniform dry-zone. Mixed morphological characters between P. bandula and P. nigrofasciata, and P. cumingii and P. reval, and their unresolved phylogenies, may suggest recent speciation scenarios with incomplete lineage sorting, or hybridization.

The stingray, a seemingly harmless cartilaginous fish, is capable of causing painful injuries and envenomation in humans. There is no known peer reviewed case report involving dogs in a veterinary journal at the time of writing this case report. Poor management of the condition or overzealous attempts to remove embedded barbs has resulted in complications in humans. This case report presents an effective approach to the treatment of stingray envenomation in dogs which is likely to be reproducible in other domestic animals. Clearly elucidated are the principles behind antibiotic therapy in the treatment of stingray injuries and the benefit of lignocaine injection in cases of embedded stingers. There is a need for case reports to enhance clinical knowledge of stingray management in domestic animals. This case report, thus, serves as an impetus for future research in this area of veterinary medicine.

New World porcupines (Erethizontidae) exhibit behaviors and possess integumentary structures, including the quills, that are used for self-defense. The North American porcupine (Erethizon dorsatum) has been well studied regarding these features; however, information is lacking for the South American Coendou species. We describe the defensive behavior and integumentary morphology of Coendou spinosus to understand the defensive strategies of this species and to compare with those reported for other species. We assessed the behaviors related to warning, defense, and escape of eight porcupines, as well as the characteristics of their pelage and quills. Furthermore, we microscopically analyzed skin samples of a roadkill adult male specimen. Similar to E. dorsatum, C. spinosus exhibited omnidirectional quill erection, revealing an aposematic color and, with their backs toward the perceived human threat, they performed quick tail and body movements to strike the hands of the human trying to capture them by the tail. Furthermore, C. spinosus presented an integumentary structure similar to that of E. dorsatum, and mechanisms to facilitate quill release when touched, penetration, and fixation in the opponent. The most distinct warning behavior noted was the vibration of the quills, which has not been reported for Erethizon. Our study confirms that, like other erethizontids, C. spinosus does not attack but exhibits warning, defense, and escape mechanisms and behaviors when threatened or touched. The dissemination of such information helps to counter the negative stigma associated with porcupines, as they can be the victims of attacks by dogs and humans, and to promote their conservation., (© 2024 Wiley Periodicals LLC.)

This study investigated the fin development and morphological characteristics according to larval growth in order to obtain information on behavioral characteristics and optimal stocking density during red seed grouper seed production. To examine the growth and fin development process of the larvae, we randomly sampled at 1, 3, 5, 7, 9, 10, 11, 13, 15, 17, 19, 20, 25, 30, 39, 45, 51, and 72 days after hatching. External morphology was observed and measured using an optical microscope. To observe skeletal development, larvae at 13, 20, 30, and 72 days after hatching were fixed in formalin and stained for cartilage and bone examination. At 9-10 DAH, red spotted grouper larvae (2.74±0.1 to 3.0±0.2 mm TL) exhibited a second dorsal fin spine and pelvic fin spine, which subsequently elongated. At 19-20 DAH, the larvae (5.7±0.1 to 6.1±0.1 mm TL) have the lengths of the second dorsal fin spine and pelvic fin spine average 34% and 31% to total length, respectively. From 30 to 72 DAH (12.6±0.4 to 56.0±0.2 mm TL), the length of the second dorsal fin spine and pelvic fin spine to total length decreased from 27% to 8% for the dorsal fin and 21% to 14% for the pelvic fin, respectively. At 30 DAH (12.6±0.4 mm TL), the larvae reached the complete count of fin rays in each fin. At 39 DAH (20.28±3.07 mm TL), the larvae had fin shapes similar to those of adults. At 13-30 DAH (4.2±0.1 to 12.6±0.9 mm TL), barbs and spinules were distributed along the ridges of the second dorsal and pelvic fin spines. However, at 72 DAH, these barbs and spinules were no longer observed on the fins. During the seed production process, red spotted grouper larvae tend to cluster in the morning, and during this time, entanglement of barbs and spinules on the second dorsal and pelvic fin spines can lead to mortality. Therefore, it is considered essential to focus on managing the behavioral patterns and appropriate rearing density of red spotted grouper larvae from the emergence of barbs and spinules on the second dorsal and pelvic fin spines until they regress and metamorphosis is completed., Competing Interests: The authors declare no potential conflict of interest., (© Copyright 2024 The Korean Society of Developmental Biology.)

Background: Sri Lanka is a continental island separated from India by the Palk Strait, a shallow-shelf sea, which was emergent during periods of lowered sea level. Its biodiversity is concentrated in its perhumid south-western 'wet zone'. The island's freshwater fishes are dominated by the Cyprinidae, characterized by small diversifications of species derived from dispersals from India. These include five diminutive, endemic species of Pethia (P. bandula, P. cumingii, P. melanomaculata, P. nigrofasciata, P. reval), whose evolutionary history remains poorly understood. Here, based on comprehensive geographic sampling, we explore the phylogeny, phylogeography and morphological diversity of the genus in Sri Lanka. Results: The phylogenetic analyses, based on mitochondrial and nuclear loci, recover Sri Lankan Pethia as polyphyletic. The reciprocal monophyly of P. bandula and P. nigrofasciata, and P. cumingii and P. reval, is not supported. Pethia nigrofasciata, P. cumingii, and P. reval show strong phylogeographic structure in the wet zone, compared with P. melanomaculata, which ranges across the dry and intermediate zones. Translocated populations of P. nigrofasciata and P. reval in the Central Hills likely originate from multiple sources. Morphological analyses reveal populations of P. nigrofasciata proximal to P. bandula, a narrow-range endemic, to have a mix of characters between the two species. Similarly, populations of P. cumingii in the Kalu basin possess orange fins, a state between the red-finned P. reval from Kelani to Deduru and yellow-finned P. cumingii from Bentara to Gin basins. Conclusions: Polyphyly in Sri Lankan Pethia suggests two or three colonizations from mainland India. Strong phylogeographic structure in P. nigrofasciata, P. cumingii and P. reval, compared with P. melanomaculata, supports a model wherein the topographically complex wet zone harbors greater genetic diversity than the topographically uniform dry-zone. Mixed morphological characters between P. bandula and P. nigrofasciata, and P. cumingii and P. reval, and their unresolved phylogenies, may suggest recent speciation scenarios with incomplete lineage sorting, or hybridization. [ABSTRACT FROM AUTHOR]

Background and Aim: The Barb horse occupies a prominent place in the history, culture, and equestrian traditions of the Maghreb (Algeria, Morocco, and Tunisia). Although many studies on the breed standard and morphometry have been published, there are no studies on its fertility and reproductive potential. Thus, this work aimed to study the fertility of Barb, Arabian Purebred, and Thoroughbred horses in Algeria. Materials and Methods: A total of 168 stallions and 1202 mares at the Chaouchaoua Stud farm in Tiaret, Algeria, were included in the study. The reproductive performance during 1592 cycles over 10 consecutive mating seasons (2003-2012) was evaluated. Multivariate analysis with logistic regression was used to analyze the reproductive parameters such as the number of cycles operated, number of pregnant mares, pregnancy rate per cycle, seasonal pregnancy rate, and embryonic mortality rate, and to determine the influence of breed, stallion's age, and year of the study on reproduction. Results: Statistical analysis showed that stallion breed was a significant influencing factor for the number of pregnant barren mares (Odds ratio [OR]=1.72; p=0.03; 95% confidence interval [CI]=1.05, 2.84) seasonal pregnancy rate (OR=1.40; p5 years on the number of pregnant foaling mares and seasonal pregnancy rate, significant interactions were observed between the stallion's breed and age, and the pregnancy rate per cycle: in the Barb breed, the pregnancy rate per cycle increased with the age of the stallion, while in the Thoroughbred, it decreased with age. Moreover, a significant effect of the year of the study on the pregnancy rate per cycle and number of pregnant foaling mares was observed. In contrast, the number of cycles and embryonic mortality rate were not influenced by the breed and age of the stallion, or the year of the study. Conclusion: The Algerian Barb horse attained a similar level of fertility compared with that of the Arabian Purebred and Thoroughbred stallions depending on its age and reproductive performance.

The aim of this study was to compare genetically Moroccan (MA) and Tunisian (TN) Barb horses and toe stablish the phylogenic relation between Barb horse and other horse breeds in Morocco by using microsatellites. First, 100 Moroccan barb and 100 Tunisian Barb horses were used. Genetic variability index between and inside the populations were estimated. 133 alleles were detected in the Moroccan population and 125 in the Tunisian. The mean number of alleles was 7.29 and 7.82 respectively for the Tunisian and Moroccan barb horse. The heterozygosity was almost similar in the two groups of Barb horses (TN = 0.716 and MA = 0.714). Fst was estimated at 0.0454 and 0.0780 respectively for TN and MA Barb horses. The Gst was also estimated at 0.012 and the Nei genetic distance at 0.05. The two populations are almost genetically similar and heterogeneous. Finally, 86,5 % of horses were correctly affected to their likelihood population. Secondly, 50 Arabian horses, 50 Arabian-Barb and 50 thoroughbreds and the 200 Barb horses previously studied were used to establish the phylogenic relationships. The use of 17 microsatellites showed that the Barb horses had specific alleles and the highest heterozygosity and the highest genetic distances were obtained with thoroughbred. The two Barb horse populations were highly combined in one side of the phylogenic tree.

Objective: To evaluate the efficacy and tolerability of a self‐expandable covered metallic stent in patients with malignant prostatic obstruction secondary to prostate cancer (PC). Methods: We reviewed 22 cases of insertion of self‐expandable covered metallic stents with barbs. Data were collected about PC status. Uroflowmetry variables, residual urine volume, International Prostate Symptom Score (IPSS), quality of life (QOL), and duration from stent insertion to removal were surveyed. These clinical parameters were compared before and after stent insertion. Results: The patients with PC showed a mean age of 75.5 ± 6.5 years and mean 5.1 ± 1.9 Charlson comorbidity index. The average flow rate (2.4 ± 1.9 vs 5.9 ± 2.4 mL/s, P =.005), peak flow rate (6.9 ± 6.2 vs 14.1 ± 5.5 mL/s, P =.003), flow time (54.6 ± 29.1 vs 23.6 ± 13.7 s, P =.002), residual urine volume (178.7 ± 195.5 vs 7.0 ± 7.1 mL, P =.004), IPSS (26.2 ± 8.1 vs 8.0 ± 6.5 points, P =.001), and QOL (4.7 ± 1.3 vs 2.4 ± 2.1 points, P =.030) improved between before and after stent insertion, respectively. Pain was the most common complication, but 60% of the patients were managed without any intervention. There were hematuria, urinary retention, urinary frequency, obstruction, and urinary incontinence. However, there was no urinary tract infection due to the stent. The median time to stent removal was 5.7 months. Conclusions: The stent was maintained for about 6 months with improved objective and subjective outcomes. The patients with PC, who had a poor comorbidity index and advanced PC status showed a tolerable maintenance period. Self‐expandable covered metallic stents can be used for PC patients with a short life expectancy and unsuitability for general anesthesia. [ABSTRACT FROM AUTHOR]

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Streambank stabilization techniques, designed to maximize localized streambank shear strength and/or minimize the forces acting on a streambank, have been in existence for centuries and are still a popular river management technique used by practitioners worldwide. The purpose of this literature review is to identify common streambank stabilization techniques, compile and summarize the recent peer-reviewed journal articles on these techniques, and determine research needs. Eleven general streambank stabilization practices, consisting of both instream structures and streambank management techniques, are identified in this literature review. Over 140 peer-reviewed journal articles on these techniques have been published over the last 20 years. To improve design and implementation of streambank stabilization techniques, two major research needs were identified: (1) further assess and quantify the spatiotemporal effects that streambank stabilization practices have on bank erosion, hydraulics, sediment transport, and habitat and (2) continue to improve numerical models for streambank stabilization design in order to holistically evaluate and address these effects. In addition, a list of specific research needs for each stabilization technique is provided. To help address these research needs, it is recommended that future streambank stabilization publications should (1) use consistent technique nomenclature, (2) provide characteristic details about the techniques and channels studied, (3) justify the experimental setup, and (4) explain how the research will improve streambank stabilization design. [ABSTRACT FROM AUTHOR]

This article examines recent innovations in how television audiences are measured, paying particular attention to the industry's growing efforts to utilize the large bodies of data generated through social media platforms – a paradigm of research known as Big Data. Although Big Data is considered by many in the television industry as a more veracious model of audience research, this essay uses Boyd and Crawford's (2011) 'Six Provocations of Big Data' to problematize and interrogate this prevailing industrial consensus. In doing so, this article explores both the affordances and the limitations of this emerging research paradigm – the latter having largely been ignored by those in the industry – and considers the consequences of these developments for the production culture of television more broadly. Although the full impact of the television industry's adoption of Big Data remains unclear, this article traces some preliminary connections between the introduction of these new measurement practices and the production culture of contemporary television. First, I demonstrate how the design of Big Data privileges real-time analysis, which, in turn, encourages increased investment in 'live' and/or 'event' television. Second, I argue that despite its potential to produce real-time insights, the scale of Big Data actually limits its utility in the context of the creative industries. Third, building on this discussion of the debatable value and applicability of Big Data, I describe how the introduction of social media metrics is further contributing to a 'data divide' in which access to these new information data sets is highly uneven, generally favouring institutions over individuals. Taken together, these three different but overlapping developments provide evidence that the introduction of Big Data is already having a notable effect on the television industry in a number of interesting and unexpected ways. [ABSTRACT FROM AUTHOR]

In this paper we present the results of a use-wear study of quartz micro-notches identified during a technological analysis of lithics from the Howiesons Poort layers of Sibudu Cave. Building on the technological analysis and preliminary functional screening of the archaeological material, a series of experiments was designed to evaluate different hypotheses for notch formation (blank production, intentional notching, hafting, projectile use, and trampling). The experimental reference collection was compared with archaeological micro-notches and a large sample of other archaeological quartz pieces (including bladelets, bipolar blanks, flakes and retouched pieces). This allowed us to evaluate the causes of micro-notch formation in the studied assemblage. Results indicate two novelties in the Howiesons Poort hunting technology at Sibudu: the use of quartz barbs and non-retouched quartz blanks. It seems that in addition to backed pieces (segments, obliquely backed points, etc.), unretouched pieces were mounted as elements in hunting weapons during the Howiesons Poort techno-tradition. Seven probable and 29 tentative barbs were identified. We thus present one of the strongest and oldest bodies of evidence for the use of barbs as projectile elements. [ABSTRACT FROM AUTHOR]

The purpose of this research is to determine the growth pattern of Barb (Barbodes balleroides) in the Jatigede reservoir, Sumedang regency Province of West Java at the period of inundation. The research was conducted using the survey method in April and June 2016. Fish identification, and data analysis was performed at Laboratory of Aquatic Resource Management Padjadjaran University. Data analysis included: size distribution, the length and weight relationship, and the condition factor using the method of Fisheries Biology. The largest groups of Barb were caught in April (sized 128-145 mm and 146-163 mm, 25% each) and June (sized 146-160 mm, 53%). Barb growth pattern in April follow the regression equation of y = 2,8753x - 4,5568 b = 2,8753, W= 3.10-5 . L2,8753, whereas in June y = 2,8105x - 4,3927, b = 2.8105, W= 4.10-5. L2,8105. The pattern of growth was allometrically negative, indicating that growth in length was greater than growth in weight. Based on the present data, it can be concluded that Barb population caught in the Reservoir at the beginning of Jatigede inundations consisted of 6 - 7 size classes. Consideration for a correct environmental management are reported. [ABSTRACT FROM AUTHOR]

Abstract Sixty bovine spongiform encephalopathy (BSE) cases of Classical or unknown type (BARB‐60 cases) were born after the date of entry into force of the EU total feed ban on 1 January 2001. The European Commission has requested EFSA to provide a scientific opinion on the most likely origin(s) of these BARB‐60 cases; whether feeding with material contaminated with the BSE agent can be excluded as the origin of any of these cases and, if so, whether there is enough scientific evidence to conclude that such cases had a spontaneous origin. The source of infection cannot be ascertained at the individual level for any BSE case, including these BARB‐60 cases, so uncertainty remains high about the origin of disease in each of these animals, but when compared with other biologically plausible sources of infection (maternal, environmental, genetic, iatrogenic), feed‐borne exposure is the most likely. This exposure was apparently excluded for only one of these BARB‐60 cases. However, there is considerable uncertainty associated with the data collected through the field investigation of these cases, due to a time span of several years between the potential exposure of the animal and the confirmation of disease, recall difficulty, and the general paucity of documented objective evidence available in the farms at the time of the investigation. Thus, feeding with material contaminated with the BSE agent cannot be excluded as the origin of any of the BARB‐60 cases, nor is it possible to definitively attribute feed as the cause of any of the BARB‐60 cases. A case of disease is classified as spontaneous by a process of elimination, excluding all other definable possibilities; with regard to the BARB‐60 cases, it is not possible to conclude that any of them had a spontaneous origin.

Microstructure of the contour feathers (remex and covert) of 9 species has been compared:Bonasa bonasia, Tetrao urogallus, Lagopus lagopus, Lyrurus tetrix, Coturnix coturnix,Perdix perdix, Francolinus francolinus, Phasianus colchichus, Gallus gallus domesticus.Feathers (one remige and one covert feather) from 3–15 specimens of each specieshave been studied. It is shown, that the length of a wing varies considerably, positivelycorrelating with the size of birds.

Worker honeybee is well-known for its stinger with microscopic backward-facing barbs for self-defense. The natural geometry of the stinger enables painless penetration and adhesion in the human skin to deliver poison. In this study, Apis cerana worker honeybee stinger and acupuncture microneedle (as a barbless stinger) were characterized by Scanning Electron Microscope (SEM). The insertion and pull process of honeybee stinger into rabbit skin was performed by a self-developed mechanical loading equipment in comparison with acupuncture needle. In order to better understand the insertion and pull mechanisms of the stinger and its barbs in human multilayer skin, a nonlinear finite element method (FEM) was conducted. Experimental results showed that the average pull-out force of the stinger was 113.50 mN and the average penetration force was only 5.75 mN. The average penetration force of the stinger was about one order of magnitude smaller than that of an acupuncture microneedle while the average pull-out force was about 70 times larger than that of an acupuncture microneedle. FEM results showed that the stress concentrations were around the stinger tip and its barbs during the insertion process. The barbs were jammed in and torn the skin during the pull process. The insertion force of the stinger was greatly minimized due to its ultrasharp stinger tip and barbs while the pull force was seriously enhanced due to the mechanical interlocking of the barbs in the skin. These excellent properties are mainly a result of optimal geometry evolved by nature. Such finding may provide an inspiration for the further design of improved tissue adhesives and micro-needles for painless transdermal drug delivery and bio-signal recording. [ABSTRACT FROM AUTHOR]

The emergence and epidemic of classical Bovine Spongiform Encephalopathy (cBSE) represents one of the most important and unique episodes in disease control of a zoonotic disease due to its novelty and its impact. Since its detection in 1986 in the United Kingdom, it has also been detected in 25 countries. The novel nature of its infectious agent and the discovery of its zoonotic potential (causing the new variant of Creutzfeldt-Jakob disease in humans) caught the food industry, policy makers, scientific community and consumers off guard, with concerns over massive human exposure and health impact. Thirty-five years later, and following the feed bans of mammalian protein to livestock in 1996, the epidemic is now in its final stages, with expectations of occasional cases emerging until the year 2026. In the last six years, two cBSE cases from animals Born After the Reinforced feed Ban (BARB) have been identified in Scotland and England, delaying their application for BSE Negligible status. This paper provides a current and historical analysis of the cBSE epidemic situation in Great Britain and review the policies implemented, its impact and the possible factors explaining the occurrence of new cases. The analysis and review reinforce the hypothesis that cBSE BARB cases occurrence may not be spontaneous, yet there remains much uncertainty of their aetiology. To date, 181,122 cBSE cases have been detected in Great Britain, of which 178 are BARB cases; and 178 human cases of the new variant of Creutzfeldt-Jakob have been diagnosed. The disease triggered major policy responses in the country, and worldwide, that have transformed the industry and our approach to animal health. Almost all its impact originated from societal reactions to the disease, from disposal of animals and products, to reduction of the national herd and its production efficiency, losses through trade restrictions and reduction in market prices and consumers' confidence, hardening of cleaning and control procedures in farms and hospitals, generation of heavy government investment plans through numerous support, surveillance and research schemes, and political and societal changes. BSE is an example of major system shock to a food industry, but which experience has resulted in better traceability systems of animals, increased capacity to develop robust diagnostic methods, numerous lessons learnt on policy coordination, implementation and communication, increased society awareness on food systems and overall improved the country's preparedness to future epidemics. [ABSTRACT FROM AUTHOR]