1. Hepatitis B virus envelope epitopes: gene assembly and expression in Escherichia coli of an immunologically reactive novel multiple-epitope polypeptide 1 (MEP-1).
- Author
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Kumar V, Bansal VJ, Rao KV, and Jameel S
- Subjects
- Amino Acid Sequence, Antigens, Viral chemistry, Antigens, Viral immunology, Base Sequence, Blotting, Western, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Epitopes chemistry, Epitopes immunology, Escherichia coli genetics, Genes, Synthetic genetics, Genes, Synthetic immunology, Hepatitis B virus chemistry, Hepatitis B virus immunology, Molecular Sequence Data, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins immunology, Viral Envelope Proteins chemistry, Viral Envelope Proteins immunology, Antigens, Viral genetics, Epitopes genetics, Hepatitis B virus genetics, Recombinant Fusion Proteins genetics, Viral Envelope Proteins genetics
- Abstract
A novel synthetic 323-bp gene with the open reading frame of a multiple-epitope polypeptide has been assembled and cloned. The gene is engineered by contiguous alignment of selected epitopes and functional domains of the hepatitis B virus envelope proteins separated by pairs of glycine residues. High-level bacterial production of this 100-amino acid (approx. 10 kDa) protein has been achieved and the gene product is stable. ELISA and Western blot experiments using epitope-specific antisera confirm that the corresponding epitopes are present in the engineered protein.
- Published
- 1992
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