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Hepatitis B virus envelope epitopes: gene assembly and expression in Escherichia coli of an immunologically reactive novel multiple-epitope polypeptide 1 (MEP-1).
- Source :
-
Gene [Gene] 1992 Jan 15; Vol. 110 (2), pp. 137-44. - Publication Year :
- 1992
-
Abstract
- A novel synthetic 323-bp gene with the open reading frame of a multiple-epitope polypeptide has been assembled and cloned. The gene is engineered by contiguous alignment of selected epitopes and functional domains of the hepatitis B virus envelope proteins separated by pairs of glycine residues. High-level bacterial production of this 100-amino acid (approx. 10 kDa) protein has been achieved and the gene product is stable. ELISA and Western blot experiments using epitope-specific antisera confirm that the corresponding epitopes are present in the engineered protein.
- Subjects :
- Amino Acid Sequence
Antigens, Viral chemistry
Antigens, Viral immunology
Base Sequence
Blotting, Western
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Epitopes chemistry
Epitopes immunology
Escherichia coli genetics
Genes, Synthetic genetics
Genes, Synthetic immunology
Hepatitis B virus chemistry
Hepatitis B virus immunology
Molecular Sequence Data
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins immunology
Viral Envelope Proteins chemistry
Viral Envelope Proteins immunology
Antigens, Viral genetics
Epitopes genetics
Hepatitis B virus genetics
Recombinant Fusion Proteins genetics
Viral Envelope Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1119
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 1371488
- Full Text :
- https://doi.org/10.1016/0378-1119(92)90640-b