24 results on '"Atalanta Ghosh"'
Search Results
2. Outpatient versus inpatient mixed meal tolerance and arginine stimulation testing yields comparable measures of variability for assessment of beta cell function
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Sudha S. Shankar, Douglas S. Lee, Ralph H. Raymond, Roberto A. Calle, Claudio Cobelli, Atalanta Ghosh, R. Paul Robertson, Hartmut Ruetten, Myrlene A. Staten, Darko Stefanovski, Adrian Vella, Sanya Whitaker, and David A. Fryburg
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Medicine (General) ,R5-920 - Abstract
Standard practice to minimize variability in beta cell function (BCF) measurement is to test in inpatient (IP) settings. IP testing strains trial subjects, investigators, and budgets. Outpatient (OP) testing may be a solution although there are few reports on OP BCF testing variability. We compared variability metrics between OP and IP from a standardized mixed meal tolerance test (MMTT) and arginine stimulation test (AST) in two separate type 2 diabetes (T2DM) cohorts (OP, n = 20; IP n = 22) in test-retest design. MMTT variables included: insulin sensitivity (Si); beta cell responsivity (Φtot); and disposition index (DItot = Si* Φtot) following 470 kCal meal. AST variables included: acute insulin response to arginine (AIRarg) and during hyperglycemia (AIRargMAX). Results: Baseline characteristics were well-matched. Between and within subject variance for each parameter across cohorts, and intraclass correlation coefficients (ICC-a measure of reproducibility) across parameters were generally comparable for OP to IP. Table summarizes the ICC results for each key parameter and cohort.Test/ParameterOutpatient (95% CI)Inpatient (95% CI)MMTT: Si0.49(0,0.69)0.28(0,0.60)MMTT: Φtot0.65(0.16,0.89)0.81(0.44,0.93)MMTT: DI0.67(0,0.83)0.36(0,0.69)AST: AIR Arg0.96(0.88,0.98)0.84(0.59,0.94)AST: AIR Arg Max0.97(0.90,0.99)0.95(0.86,0.97)AST: ISR0.93(0.77,0.97)0.93(0.82,0.96)In conclusion, the variability (reproducibility) of BCF measures from standardized MMTT and AST is comparable between OP and IP settings. These observations have significant implications for complexity and cost of metabolic studies.
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- 2018
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3. Pharmacodynamic effects of canagliflozin, a sodium glucose co-transporter 2 inhibitor, from a randomized study in patients with type 2 diabetes.
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Sue Sha, Damayanthi Devineni, Atalanta Ghosh, David Polidori, Marcus Hompesch, Sabine Arnolds, Linda Morrow, Heike Spitzer, Keith Demarest, and Paul Rothenberg
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Medicine ,Science - Abstract
This randomized, double-blind, placebo-controlled, single and multiple ascending-dose study evaluated the pharmacodynamic effects and safety/tolerability of canagliflozin, a sodium glucose co-transporter 2 inhibitor, in patients with type 2 diabetes.Patients (N = 116) discontinued their antihyperglycemic medications 2 weeks before randomization. Patients received canagliflozin 30, 100, 200, or 400 mg once daily or 300 mg twice daily, or placebo at 2 study centers in the United States and Germany, or canagliflozin 30 mg once daily or placebo at 1 study center in Korea, while maintaining an isocaloric diet for 2 weeks. On Days -1, 1, and 16, urinary glucose excretion (UGE), plasma glucose (PG), fasting PG (FPG), and insulin were measured. The renal threshold for glucose (RTG) was calculated from UGE, PG, and estimated glomerular filtration rate. Safety was evaluated based on adverse event (AE) reports, vital signs, electrocardiograms, clinical laboratory tests, and physical examinations.Canagliflozin increased UGE dose-dependently (∼80-120 g/day with canagliflozin ≥100 mg), with increases maintained over the 14-day dosing period with each dose. Canagliflozin dose-dependently decreased RTG, with maximal reductions to ∼4-5 mM (72-90 mg/dL). Canagliflozin also reduced FPG and 24-hour mean PG; glucose reductions were seen on Day 1 and maintained over 2 weeks. Plasma insulin reductions with canagliflozin were consistent with observed PG reductions. Canagliflozin also reduced body weight. AEs were transient, mild to moderate in intensity, and balanced across groups; 1 canagliflozin-treated female reported an episode of vaginal candidiasis. Canagliflozin did not cause hypoglycemia, consistent with the RTG values remaining above the hypoglycemia threshold. At Day 16, there were no clinically meaningful changes in urine volume, urine electrolyte excretion, renal function, or routine laboratory test values.Canagliflozin increased UGE and decreased RTG, leading to reductions in PG, insulin, and body weight, and was generally well tolerated in patients with type 2 diabetes.ClinicalTrials.gov NCT00963768.
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- 2014
- Full Text
- View/download PDF
4. A Phase 1, Multicenter, Open-Label Study to Evaluate the Pharmacokinetics of Iberdomide in Subjects with Mild, Moderate, or Severe Hepatic Impairment Compared with Healthy Subjects
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Yiming Cheng, Ying Ye, Allison Gaudy, Atalanta Ghosh, Yongjun Xue, Alice Wang, Simon Zhou, and Yan Li
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Pharmacology (medical) ,Advances and Applications [Clinical Pharmacology] - Abstract
Yiming Cheng,1 Ying Ye,1 Allison Gaudy,1 Atalanta Ghosh,2 Yongjun Xue,3 Alice Wang,1 Simon Zhou,1 Yan Li1 1Clinical Pharmacology & Pharmacometrics, Bristol Myers Squibb, Princeton, NJ, USA; 2Global Biometrics and Data Sciences, Bristol Myers Squibb, Princeton, NJ, USA; 3Nonclinical Research & Development, Bristol Myers Squibb, Princeton, NJ, USACorrespondence: Yan Li, Clinical Pharmacology & Pharmacometrics, Bristol Myers Squibb, 556 Morris Ave, Summit, Princeton, NJ, 07901, USA, Tel +1 908-481-6203, Email Yan.Li@bms.comIntroduction: Iberdomide, a novel cereblon modulator (CELMoD®), is currently under clinical investigation for hematology indications. To evaluate the influence of hepatic impairment on the pharmacokinetics (PK) of iberdomide and its major active metabolite M12, a phase 1, multicenter, open-label study was conducted in healthy subjects and subjects with mild, moderate, and severe hepatic impairment.Methods: Forty subjects were enrolled in the study and divided into five groups based on hepatic function. 1 mg iberdomide was administered and plasma samples were collected to evaluate the pharmacokinetics of iberdomide and M12.Results: After a single dose of iberdomide (1 mg), mean iberdomide Cmax (maximum observed concentration) and AUC (area under the concentration-time curve) exposure were generally comparable between hepatic impairment (HI) subjects (severe, moderate and mild) and their respective matched normal controls. Mean Cmax and AUC exposure of the metabolite M12 were generally comparable between mild HI and matched normal subjects. However, mean Cmax of the M12 was 30% and 65% lower and AUC was 57% and 63% lower in moderate and severe HI subjects as compared to their respective matched normal controls. However, given the relatively low M12 exposure as compared to its parent drug, the observed differences were not considered clinically meaningful.Conclusion: In summary, 1 mg single oral dose of iberdomide was generally well-tolerated. HI (mild, moderate or severe) had no clinically relevant impact on iberdomide PK and therefore, no dose adjustment is warranted.Keywords: hepatic impairment, iberdomide, pharmacokinetics
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- 2023
5. Safety, Pharmacokinetics, and Pharmacodynamics of CC‐90001 (BMS‐986360), a c‐Jun N‐terminal Kinase Inhibitor, in Phase 1 Studies in Healthy Participants
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Ying, Ye, Allison, Gaudy, Michael, Thomas, Josephine, Reyes, Barbara, Burkhardt, Gerald, Horan, Liangang, Liu, Jian, Chen, Atalanta, Ghosh, Leonidas N, Carayannopoulos, Daniel A, Tatosian, and Maria, Palmisano
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Adult ,Dose-Response Relationship, Drug ,Double-Blind Method ,JNK Mitogen-Activated Protein Kinases ,Humans ,Pharmaceutical Science ,Pharmacology (medical) ,Healthy Volunteers ,Half-Life - Abstract
CC-90001 selectively inhibits c-Jun N-terminal kinase (JNK), a stress-activated protein implicated in fibrosis. In 3 phase 1 trials evaluating CC-90001 pharmacokinetics, pharmacodynamics, and safety, healthy adults (N = 184) received oral CC-90001 in a single dose (10-720 mg) or multiple doses (30-480 mg once daily for 7-18 days) or placebo. CC-90001 was rapidly absorbed (median time to maximum concentration, 1-4 hours) and eliminated with a mean terminal elimination half-life of 12-28 hours. Steady state was reached on day 5, with a mean accumulation ratio of 1.5- to 2-fold following daily dosing. Exposure was similar in fed versus fasted participants and in Japanese versus non-Japanese participants. CC-90001 demonstrated dose- and exposure-dependent inhibition of JNK as determined by histopathological analysis of c-Jun phosphorylation in ultraviolet-irradiated skin. The most common treatment-emergent adverse events were nausea and headache; all were mild or moderate in intensity. Based on exposure-response analysis using high-quality electrocardiogram data, no clinically relevant QT prolongation liability for CC-90001 was observed. Overall, single- and multiple-dose CC-90001 were generally safe and well tolerated at the tested doses and demonstrated JNK pathway engagement. These results support further clinical evaluation of CC-90001.
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- 2022
6. Multiple-Dose Pharmacokinetics of Ozanimod and its Major Active Metabolites and the Pharmacodynamic and Pharmacokinetic Interactions with Pseudoephedrine, a Sympathomimetic Agent, in Healthy Subjects
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Peijin Zhang, Sarah Harris, Maria Palmisano, Xiaomin Wang, Susan Walker, Jonathan Q. Tran, Atalanta Ghosh, and Mary Syto
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Adult ,Male ,030213 general clinical medicine ,Drug interaction ,Administration, Oral ,Blood Pressure ,Pharmacology ,Placebo ,MAO-B ,Ozanimod ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Double-Blind Method ,Pharmacokinetics ,In vivo ,polycyclic compounds ,Humans ,Medicine ,Pharmacology (medical) ,Sympathomimetics ,Active metabolite ,Original Research ,Oxadiazoles ,business.industry ,Monoamine oxidase ,General Medicine ,Middle Aged ,biochemical phenomena, metabolism, and nutrition ,Pseudoephedrine ,Healthy Volunteers ,Blood pressure ,Therapeutic Equivalency ,Delayed-Action Preparations ,030220 oncology & carcinogenesis ,Pharmacodynamics ,Indans ,Female ,business ,medicine.drug - Abstract
Introduction The aims of this study were to characterize the multiple-dose pharmacokinetics (PK) of ozanimod’s major active metabolites (CC112273 and CC1084037) and to evaluate the pharmacodynamic and PK interactions with pseudoephedrine (PSE). Methods In this phase 1, single-center, randomized, double-blind, placebo-controlled study, 56 healthy adult subjects were randomized to receive either placebo or ozanimod once daily for 30 days (0.23 mg on days 1–4, 0.46 mg on days 5–7, 0.92 mg on days 8–10, and 1.84 mg on days 11–30). On day 30, a single oral dose of PSE 60 mg was co-administered with placebo or ozanimod. Maximum time-matched change in systolic blood pressure (SBP) from baseline (day 29) following PSE administration on day 30 was calculated. Plasma PK parameters for ozanimod, CC112273, CC1084037, and PSE were estimated using noncompartmental methods. Results Fifty-two subjects (92.9%) completed the study. Following multiple dosing, approximately 94% of circulating total active drug exposure was represented by ozanimod (6%), CC112273 (73%), and CC1084037 (15%). Exposures of CC112273 and CC1084037 were highly correlated. Mean maximum time-matched change from baseline for SBP was not significantly different between ozanimod + PSE and placebo + PSE. Ozanimod also had no effect on the PK of PSE. Co-administration of ozanimod with a single dose of PSE in healthy subjects was generally well tolerated. While CC112273 and CC1084037 selectively inhibited monoamine oxidase (MAO)-B in vitro, both active metabolites do not inhibit platelet MAO-B activity in vivo. Conclusion Concomitant administration of ozanimod with PSE, a sympathomimetic agent, did not potentiate the effects on blood pressure. Trial Registration NCT03644576.
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- 2020
7. Effect of canagliflozin treatment on hepatic triglyceride content and glucose metabolism in patients with type 2 diabetes
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Kenneth Cusi, Sue Sha, Diana Barb, Nishanth E. Sunny, David Polidori, Jeremy Pettus, Robert R. Henry, Srilaxmi Kalavalapalli, Sunder Mudaliar, Fernando Bril, Theodore P. Ciaraldi, Kristin Farrell, and Atalanta Ghosh
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Male ,medicine.medical_specialty ,Proton Magnetic Resonance Spectroscopy ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,030209 endocrinology & metabolism ,Type 2 diabetes ,030204 cardiovascular system & hematology ,Carbohydrate metabolism ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Insulin resistance ,Double-Blind Method ,Weight loss ,Insulin-Secreting Cells ,Internal medicine ,Insulin Secretion ,Weight Loss ,Internal Medicine ,medicine ,Humans ,Canagliflozin ,Sodium-Glucose Transporter 2 Inhibitors ,Triglycerides ,Aged ,Glycated Hemoglobin ,Triglyceride ,business.industry ,Insulin ,Fatty liver ,Middle Aged ,medicine.disease ,Treatment Outcome ,Diabetes Mellitus, Type 2 ,Liver ,chemistry ,Glucose Clamp Technique ,Female ,Insulin Resistance ,medicine.symptom ,business ,medicine.drug - Abstract
AIM To evaluate the impact of the sodium glucose co-transporter 2 inhibitor canagliflozin on intrahepatic triglyceride (IHTG) accumulation and its relationship to changes in body weight and glucose metabolism. MATERIALS AND METHODS In this double-blind, parallel-group, placebo-controlled, 24-week trial subjects with inadequately controlled type 2 diabetes mellitus (T2DM; HbA1c = 7.7% ± 0.7%) from two centres were randomly assigned (1:1) to canagliflozin 300 mg or placebo. We measured IHTG by proton-magnetic resonance spectroscopy (primary outcome), hepatic/muscle/adipose tissue insulin sensitivity during a 2-step euglycaemic insulin clamp, and beta-cell function during a mixed meal tolerance test. Analyses were per protocol. RESULTS Between 8 September 2014-13 June 2016, 56 patients were enrolled. Canagliflozin reduced HbA1c (placebo-subtracted change: -0.71% [-1.08; -0.33]) and body weight (-3.4% [-5.4; -1.4]; both P ≤ 0.001). A numerically larger absolute decrease in IHTG occurred with canagliflozin (-4.6% [-6.4; -2.7]) versus placebo (-2.4% [-4.2; -0.6]; P = 0.09). In patients with non-alcoholic fatty liver disease (n = 37), the decrease in IHTG was -6.9% (-9.5; -4.2) versus -3.8% (-6.3; -1.3; P = 0.05), and strongly correlated with the magnitude of weight loss (r = 0.69, P < 0.001). Body weight loss ≥5% with a ≥30% relative reduction in IHTG occurred more often with canagliflozin (38% vs. 7%, P = 0.009). Hepatic insulin sensitivity improved with canagliflozin (P < 0.01), but not muscle or adipose tissue insulin sensitivity. Beta-cell glucose sensitivity, insulin clearance, and disposition index improved more with canagliflozin (P < 0.05). CONCLUSIONS Canagliflozin improves hepatic insulin sensitivity and insulin secretion and clearance in patients with T2DM. IHTG decreases in proportion to the magnitude of body weight loss, which tended to be greater and occur more often with canagliflozin.
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- 2019
8. Single-Dose Pharmacokinetics of Ozanimod and its Major Active Metabolites Alone and in Combination with Gemfibrozil, Itraconazole, or Rifampin in Healthy Subjects: A Randomized, Parallel-Group, Open-Label Study
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Xiaomin Wang, Liangang Liu, Mary Syto, Maria Palmisano, Atalanta Ghosh, Jonathan Q. Tran, and Peijin Zhang
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Itraconazole ,CYP3A ,Metabolite ,Cmax ,Pharmacology ,chemistry.chemical_compound ,Pharmacokinetics ,medicine ,Gemfibrozil ,Humans ,Pharmacology (medical) ,Drug Interactions ,Active metabolite ,Oxadiazoles ,Cross-Over Studies ,business.industry ,General Medicine ,Drug interaction ,Healthy Volunteers ,chemistry ,Area Under Curve ,Indans ,Rifampin ,business ,medicine.drug - Abstract
The aims of this study were to characterize the single-dose pharmacokinetics (PK) of the major active metabolites of ozanimod, CC112273 and CC1084037, and to evaluate the effect of gemfibrozil (a strong inhibitor of cytochrome P450 [CYP] 2C8), itraconazole (a strong inhibitor of CYP3A and P-glycoprotein [P-gp]), and rifampin (a strong inducer of CYP3A/P-gp and moderate inducer of CYP2C8) on the single-dose PK of ozanimod and its major active metabolites in healthy subjects. This was a phase 1, randomized, parallel-group, open-label study with two parts. In part 1, 40 subjects were randomized to receive a single oral dose of ozanimod 0.46 mg (group A, n = 20) or oral doses of gemfibrozil 600 mg twice daily for 17 days with a single oral dose of ozanimod 0.46 mg on day 4 (group B, n = 20). In part 2, 60 subjects were randomized to receive a single oral dose of ozanimod 0.92 mg (group C, n = 20), oral doses of itraconazole 200 mg once daily for 17 days with a single oral dose of ozanimod 0.92 mg on day 4 (group D, n = 20), or oral doses of rifampin 600 mg once daily for 21 days with a single oral dose of ozanimod 0.92 mg on day 8 (group E, n = 20). Plasma PK parameters for ozanimod, CC112273, and CC1084037 were estimated using noncompartmental methods. Dose-proportional increases in maximum observed concentration (Cmax) and area under the concentration–time curve (AUC) were observed for ozanimod, CC112273, and CC1084037. The mean terminal elimination half-life (t1/2) for ozanimod was approximately 20–22 h while the mean t1/2 for CC112273 and CC1084037 were approximately 10 days. CC112273 and CC1084037 exposures were highly correlated with or without interacting drugs. Itraconazole increased ozanimod AUC by approximately 13% while rifampin reduced ozanimod AUC by approximately 24%, suggesting a minor role of CYP3A and P-gp in the overall disposition of ozanimod. Gemfibrozil increased the AUC for CC112273 and CC1084037 by approximately 47% and 69%, respectively. Rifampin reduced the AUC for CC112273 and CC1084037, primarily via CYP2C8 induction, by approximately 60% and 55%, respectively. Ozanimod’s major active metabolites, CC112273 and CC1084037, exhibited similar single-dose PK properties and their exposures were highly correlated. CYP2C8 is one of the important enzymes in the overall disposition of CC112273 and subsequently its direct metabolite CC1084037. Clinical trial: NCT03624959
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- 2020
9. Mixed Meal and Intravenous L-Arginine Tests Both Stimulate Incretin Release Across Glucose Tolerance in Man: Lack of Correlation with β Cell Function
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Hartmut Ruetten, Mathias Gebauer, Ralph H. Raymond, Roberto A. Calle, Claudio Cobelli, Atalanta Ghosh, R. Paul Robertson, Sudha S. Shankar, Myrlene A. Staten, Darko Stefanovski, Adrian Vella, Kathryn Wright, David A. Fryburg, Richard Bergman, Roberto Calle, Mark Farmen, Atalanta Gosh, Ilan Irony, Douglas Lee, Frank Martin, Malene Hersloev, Kolaczynski Jerzy, Stephanie Moran, David Polidori, Ralph Raymond, Sudha Shankar, Myrlene Staten, Lilit Vardanian, Gordon Weir, Marjorie Zakaria, Mark Deeg, David Kelley, Peter Savage, Nicole Spear, Maria Vassileva, and Sanya Whitaker
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Blood Glucose ,0301 basic medicine ,endocrine system ,medicine.medical_specialty ,β cell function ,Time Factors ,Arginine ,Enteroendocrine Cells ,Endocrinology, Diabetes and Metabolism ,Incretin ,030209 endocrinology & metabolism ,Gastric Inhibitory Polypeptide ,Type 2 diabetes ,Mixed meal ,Glucagon ,Gastrointestinal Hormones ,Prediabetic State ,Eating ,03 medical and health sciences ,0302 clinical medicine ,Glucagon-Like Peptide 1 ,Insulin-Secreting Cells ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Insulin ,Peptide YY ,Insulin secretion ,business.industry ,digestive, oral, and skin physiology ,Correction ,Postprandial Period ,medicine.disease ,United States ,Cross-Sectional Studies ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,Administration, Intravenous ,business ,Biomarkers ,hormones, hormone substitutes, and hormone antagonists - Abstract
The aims of this study were to 1. define the responses of glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), glucagon, and peptide YY (PYY) to an oral meal and to intravenous L-arginine; and 2. examine correlation of enteroendocrine hormones with insulin secretion. We hypothesized a relationship between circulating incretin concentrations and insulin secretion.Subjects with normal glucose tolerance (NGT, n = 23), prediabetes (PDM, n = 17), or with type 2 diabetes (T2DM, n = 22) were studied twice, following a mixed test meal (470 kCal) (mixed meal tolerance test [MMTT]) or intravenous L-arginine (arginine maximal stimulation test [AST], 5 g). GLP-1 (total and active), PYY, GIP, glucagon, and β cell function were measured before and following each stimulus.Baseline enteroendocrine hormones differed across the glucose tolerance (GT) spectrum, T2DM generallyNGT and PDM. In response to MMTT, total and active GLP-1, GIP, glucagon, and PYY increased in all populations. The incremental area-under-the-curve (0-120 min) of analytes like total GLP-1 were often higher in T2DM compared with NGT and PDM (35-51%; P 0.05). At baseline glucose, L-arginine increased total and active GLP-1 and glucagon concentrations in all GT populations (all P 0.05). As expected, the MMTT and AST provoked differential glucose, insulin, and C-peptide responses across GT populations. Baseline or stimulated enteroendocrine hormone concentrations did not consistently correlate with either measure of β cell function.Both MMTT and AST resulted in insulin and enteroendocrine hormone responses across GT populations without consistent correlation between release of incretins and insulin, which is in line with other published research. If a defect is in the enteroendocrine/β cell axis, it is probably reduced response to rather than diminished secretion of enteroendocrine hormones.
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- 2018
10. Outpatient versus inpatient mixed meal tolerance and arginine stimulation testing yields comparable measures of variability for assessment of beta cell function
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Claudio Cobelli, Adrian Vella, Sanya Whitaker, Sudha S. Shankar, Darko Stefanovski, David A. Fryburg, Ralph H. Raymond, Hartmut Ruetten, Atalanta Ghosh, R. Paul Robertson, Douglas S. Lee, Myrlene A. Staten, and Roberto A. Calle
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0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Meal ,Reproducibility ,lcsh:R5-920 ,Arginine ,business.industry ,Intraclass correlation ,Beta-cell Function ,030209 endocrinology & metabolism ,Stimulation ,General Medicine ,Type 2 diabetes ,medicine.disease ,Gastroenterology ,Article ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Internal medicine ,Cohort ,medicine ,business ,lcsh:Medicine (General) - Abstract
Standard practice to minimize variability in beta cell function (BCF) measurement is to test in inpatient (IP) settings. IP testing strains trial subjects, investigators, and budgets. Outpatient (OP) testing may be a solution although there are few reports on OP BCF testing variability. We compared variability metrics between OP and IP from a standardized mixed meal tolerance test (MMTT) and arginine stimulation test (AST) in two separate type 2 diabetes (T2DM) cohorts (OP, n = 20; IP n = 22) in test-retest design. MMTT variables included: insulin sensitivity (Si); beta cell responsivity (Φtot); and disposition index (DItot = Si* Φtot) following 470 kCal meal. AST variables included: acute insulin response to arginine (AIRarg) and during hyperglycemia (AIRargMAX). Results Baseline characteristics were well-matched. Between and within subject variance for each parameter across cohorts, and intraclass correlation coefficients (ICC-a measure of reproducibility) across parameters were generally comparable for OP to IP. Table summarizes the ICC results for each key parameter and cohort. Test/Parameter Outpatient (95% CI) Inpatient (95% CI) MMTT: Si 0.49(0,0.69) 0.28(0,0.60) MMTT: Φtot 0.65(0.16,0.89) 0.81(0.44,0.93) MMTT: DI 0.67(0,0.83) 0.36(0,0.69) AST: AIR Arg 0.96(0.88,0.98) 0.84(0.59,0.94) AST: AIR Arg Max 0.97(0.90,0.99) 0.95(0.86,0.97) AST: ISR 0.93(0.77,0.97) 0.93(0.82,0.96) In conclusion, the variability (reproducibility) of BCF measures from standardized MMTT and AST is comparable between OP and IP settings. These observations have significant implications for complexity and cost of metabolic studies.
- Published
- 2018
11. Exosite 1 thrombin inhibition with JNJ-64179375 inhibits thrombus formation in a human translational model of thrombosis
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David E. Newby, Gary Peters, Simon Wilson, Thomas M Connolly, Maureen Johnson, and Atalanta Ghosh
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0301 basic medicine ,Male ,Physiology ,030204 cardiovascular system & hematology ,Pharmacology ,0302 clinical medicine ,Medicine ,Bivalirudin ,Novel ,JNJ-9375 ,Cross-Over Studies ,medicine.diagnostic_test ,Hirudins ,Healthy Volunteers ,Recombinant Proteins ,P-Selectin ,Coagulation ,Female ,Blood Coagulation Tests ,Cardiology and Cardiovascular Medicine ,medicine.drug ,Partial thromboplastin time ,Adult ,Blood Platelets ,Exosite 1 thrombin ,Thrombin time ,Antithrombins ,03 medical and health sciences ,Young Adult ,Thrombin ,Double-Blind Method ,Physiology (medical) ,Humans ,Platelet activation ,Thrombus ,Blood Coagulation ,Prothrombin time ,Fibrin ,Dose-Response Relationship, Drug ,business.industry ,Anticoagulant ,Thrombosis ,Original Articles ,medicine.disease ,Platelet Activation ,Peptide Fragments ,030104 developmental biology ,Scotland ,business ,Biomarkers - Abstract
AimsJNJ-64179375 (hereafter JNJ-9375) is a first-in-class, highly specific, large molecule, exosite 1 thrombin inhibitor. In preclinical studies, JNJ-9375 demonstrated robust antithrombotic protection with a wider therapeutic index when compared to apixaban. The purpose of the present study was to examine for the first time the antiplatelet, anticoagulant and antithrombotic effects of JNJ-9375 in a translational model of ex vivo human thrombosis.Methods and resultsFifteen healthy volunteers participated in a double-blind randomized crossover study of JNJ-9375 (2.5, 25, and 250 μg/mL), bivalirudin (6 μg/mL; positive control), and matched placebo. Coagulation, platelet activation, and thrombus formation were determined using coagulation assays, flow cytometry, and an ex vivo perfusion chamber, respectively.JNJ-9375 caused concentration-dependent prolongation of all measures of blood coagulation (prothrombin time, activated partial thromboplastin time, and thrombin time; P ConclusionExosite 1 inhibition with JNJ-9375 caused prolongation of blood coagulation, selective inhibition of thrombin-mediated platelet activation, and reductions in ex vivo thrombosis driven by a decrease in fibrin-rich thrombus formation. JNJ-9375 represents a novel class of anticoagulant with potential therapeutic applications.
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- 2018
12. Canagliflozin Lowers Postprandial Glucose and Insulin by Delaying Intestinal Glucose Absorption in Addition to Increasing Urinary Glucose Excretion
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Kristin Farrell, Robert R. Henry, Theodore P. Ciaraldi, Nicole Vaccaro, Sunder Mudaliar, David Polidori, Paul Rothenberg, Atalanta Ghosh, and Sue Sha
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Glycosuria ,Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Thiophenes ,Intestinal absorption ,Placebos ,Sodium-Glucose Transporter 1 ,Glucosides ,Sodium-Glucose Transporter 2 ,Internal medicine ,Internal Medicine ,Medicine ,Humans ,Insulin ,Canagliflozin ,Sodium-Glucose Transporter 2 Inhibitors ,Pancreatic hormone ,Original Research ,Acetaminophen ,Advanced and Specialized Nursing ,Cross-Over Studies ,Gastric emptying ,business.industry ,Area under the curve ,Clinical Care/Education/Nutrition/Psychosocial Research ,Postprandial Period ,Endocrinology ,Postprandial ,Glucose ,Gastric Emptying ,Intestinal Absorption ,Depression, Chemical ,medicine.symptom ,business ,medicine.drug - Abstract
OBJECTIVE Canagliflozin, a sodium glucose cotransporter (SGLT) 2 inhibitor, is also a low-potency SGLT1 inhibitor. This study tested the hypothesis that intestinal canagliflozin levels postdose are sufficiently high to transiently inhibit intestinal SGLT1, thereby delaying intestinal glucose absorption. RESEARCH DESIGN AND METHODS This two-period, crossover study evaluated effects of canagliflozin on intestinal glucose absorption in 20 healthy subjects using a dual-tracer method. Placebo or canagliflozin 300 mg was given 20 min before a 600-kcal mixed-meal tolerance test. Plasma glucose, 3H-glucose, 14C-glucose, and insulin were measured frequently for 6 h to calculate rates of appearance of oral glucose (RaO) in plasma, endogenous glucose production, and glucose disposal. RESULTS Compared with placebo, canagliflozin treatment reduced postprandial plasma glucose and insulin excursions (incremental 0- to 2-h area under the curve [AUC0–2h] reductions of 35% and 43%, respectively; P < 0.001 for both), increased 0- to 6-h urinary glucose excretion (UGE0–6h, 18.2 ± 5.6 vs. CONCLUSIONS Canagliflozin reduces postprandial plasma glucose and insulin by increasing UGE (via renal SGLT2 inhibition) and delaying RaO, likely due to intestinal SGLT1 inhibition.
- Published
- 2013
13. Haemodynamic effects, safety, and pharmacokinetics of human stresscopin in heart failure with reduced ejection fraction†
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Lawrence I. Deckelbaum, Orlando F. Bueno, Piotr Ponikowski, Waldemar Radziszewski, Aldo P. Maggioni, Jacek Grzybowski, Atalanta Ghosh, Stephen J. Greene, Ayan R. Patel, Allan Olson, Cezar Macarie, Marco Metra, Mihai Gheorghiade, Andreas Koester, Serban I. Bubenek-Turconi, Marvin A. Konstam, Jerzy Korewicki, and Lilian Y. Li
- Subjects
Adult ,Male ,Infusions ,Cardiac output ,Corticotropin-Releasing Hormone ,Human stresscopin ,Cardiac index ,Heart failure ,Blood Pressure ,Corticotropin-releasing factor type 2 receptor ,Dose-Response Relationship ,Double-Blind Method ,Heart Rate ,Heart rate ,medicine ,Humans ,Pharmacokinetics ,Pulmonary Wedge Pressure ,Cardiac Output ,Infusions, Intravenous ,Pulmonary wedge pressure ,Urocortins ,Aged ,Ejection fraction ,Dose-Response Relationship, Drug ,Haemodynamics ,Biological Markers ,Female ,Heart Failure ,Hemodynamics ,Middle Aged ,Stroke Volume ,business.industry ,medicine.disease ,Blood pressure ,medicine.anatomical_structure ,Anesthesia ,Vascular resistance ,Drug ,Intravenous ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers - Abstract
Aims Human stresscopin is a corticotropin-releasing factor (CRF) type 2 receptor (CRFR2) selective agonist and a member of the CRF peptide family. Stimulation of CRFR2 improves cardiac output and left ventricular ejection fraction (LVEF) in patients with stable heart failure (HF) with reduced LVEF. We examined the safety, pharmacokinetics, and effects on haemodynamics and serum biomarkers of intravenous human stresscopin acetate (JNJ-9588146) in patients with stable HF with LVEF ≤35% and cardiac index (CI) ≤2.5 L/min/m2. Methods and results Sixty-two patients with HF and LVEF ≤35% were instrumented with a pulmonary artery catheter and randomly assigned (ratio 3:1) to receive an intravenous infusion of JNJ-9588146 or placebo. The main study was an ascending dose study of three doses (5, 15, and 30 ng/kg/min) of study drug or placebo administered in sequential 1 h intervals (3 h total). Statistically significant increases in CI and reduction in systemic vascular resistance (SVR) were observed with both the 15 ng/kg/min (2 h time point) and 30 ng/kg/min (3 h time point) doses of JNJ-9588146 without significant changes in heart rate (HR) or systolic blood pressure (SBP). No statistically significant reductions in pulmonary capillary wedge pressure (PCWP) were seen with any dose tested in the primary analysis, although a trend towards reduction was seen. Conclusion In HF patients with reduced LVEF and CI, ascending doses of JNJ-9588146 were associated with progressive increases in CI and reductions in SVR without significant effects on PCWP, HR, or SBP. Trial registration: NCT01120210
- Published
- 2013
14. Validation of a Novel Method for Determining the Renal Threshold for Glucose Excretion in Untreated and Canagliflozin-treated Subjects With Type 2 Diabetes Mellitus
- Author
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Sue Sha, Atalanta Ghosh, David Polidori, Paul Rothenberg, Tim Heise, and Leona Plum-Mörschel
- Subjects
Glycosuria ,Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Renal function ,Context (language use) ,Thiophenes ,Kidney ,Kidney Function Tests ,Biochemistry ,Body Mass Index ,Endocrinology ,Glucosides ,Internal medicine ,Diabetes mellitus ,Membrane Transport Modulators ,medicine ,Humans ,Hypoglycemic Agents ,Canagliflozin ,Sodium-Glucose Transporter 2 Inhibitors ,business.industry ,Biochemistry (medical) ,Type 2 Diabetes Mellitus ,Kidney metabolism ,JCEM Online: Brief Reports ,Middle Aged ,Overweight ,medicine.disease ,Postprandial Period ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,Renal threshold ,Female ,medicine.symptom ,business ,medicine.drug ,Glomerular Filtration Rate - Abstract
Context: The stepwise hyperglycemic clamp procedure (SHCP) is the gold standard for measuring the renal threshold for glucose excretion (RTG), but its use is limited to small studies in specialized laboratories. Objective: The objective of the study was to validate a new method for determining RTG using data obtained during a mixed-meal tolerance test (MMTT) in untreated and canagliflozin-treated subjects with type 2 diabetes mellitus (T2DM). Design: This was an open-label study with 2 sequential parts. Setting: The study was performed at a single center in Germany. Patients: Twenty-eight subjects with T2DM were studied. Interventions: No treatment intervention was given in part 1. In part 2, subjects were treated with canagliflozin 100 mg/d for 8 days. In each part, subjects underwent an MMTT and a 5-step SHCP on consecutive days. Main Outcome Measures: For both methods, RTG was estimated using measured blood glucose (BG) and urinary glucose excretion (UGE); estimated glomerular filtration rates were also used to determine RTG during the MMTT. The methods were compared using the concordance correlation coefficient and geometric mean ratios. Results: In untreated and canagliflozin-treated subjects, the relationship between UGE rate and BG was well described by a threshold relationship. Good agreement was obtained between the MMTT-based and SHCP-derived RTG values. The concordance correlation coefficient (for all subjects) was 0.94; geometric mean ratios (90% confidence intervals) for RTG values (MMTT/SHCP) were 0.93 (0.89–0.96) in untreated subjects and 1.03 (0.78–1.37) in canagliflozin-treated subjects. Study procedures and treatments were generally well tolerated in untreated and canagliflozin-treated subjects. Conclusions: In both untreated and canagliflozin-treated subjects with T2DM, RTG can be accurately estimated from measured BG, UGE, and estimated glomerular filtration rates using an MMTT-based method.
- Published
- 2013
15. Pharmacodynamic differences between canagliflozin and dapagliflozin: results of a randomized, double-blind, crossover study
- Author
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J. Pinheiro, Nicole Vaccaro, L. Plum-Mörschel, Kristin Farrell, Atalanta Ghosh, Paul Rothenberg, Sue Sha, David Polidori, and Jaya Natarajan
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,phase I–II study ,Type 2 diabetes ,Thiophenes ,Kidney ,Drug Administration Schedule ,Excretion ,chemistry.chemical_compound ,Endocrinology ,Double-Blind Method ,Glucosides ,Internal medicine ,Internal Medicine ,medicine ,pharmacodynamics ,Humans ,Hypoglycemic Agents ,Dapagliflozin ,Benzhydryl Compounds ,Canagliflozin ,Aged ,Cross-Over Studies ,business.industry ,Body Weight ,SGLT2 inhibitor ,Original Articles ,dapagliflozin ,Middle Aged ,medicine.disease ,Postprandial Period ,Crossover study ,Postprandial ,Glucose ,chemistry ,Pharmacodynamics ,Renal threshold ,Female ,Original Article ,type 2 diabetes ,business ,medicine.drug ,Glomerular Filtration Rate - Abstract
Aims To compare the pharmacodynamic effects of the highest approved doses of the sodium glucose co-transporter 2 (SGLT2) inhibitors canagliflozin and dapagliflozin on urinary glucose excretion (UGE), renal threshold for glucose excretion (RTG) and postprandial plasma glucose (PPG) excursion in healthy participants in a randomized, double-blind, two-period crossover study. Methods In each treatment period, participants (n = 54) received canagliflozin 300 mg or dapagliflozin 10 mg for 4 days (20 min before breakfast). A mixed-meal tolerance test (600 kcal; 75 g glucose) was performed at baseline and on day 4 of each treatment period to assess changes in incremental PPG (PPGΔAUC0–2 h). We measured 24-h UGE and plasma glucose on day 4 to determine 24-h mean RTG. Results Canagliflozin 300 mg and dapagliflozin 10 mg had similar effects on UGE and RTG for 4 h after dosing, but canagliflozin was associated with higher UGE and greater RTG reductions for the remainder of the day. Mean 24-h UGE was ∼25% higher with canagliflozin than with dapagliflozin (51.4 vs. 40.8 g), and 24-h mean RTG was ∼0.4 mmol/l (7 mg/dl) lower with canagliflozin than with dapagliflozin (3.79 vs. 4.17 mmol/l; p
- Published
- 2014
16. Arginine is preferred to glucagon for stimulation testing of β-cell function
- Author
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R Paul, Robertson, Ralph H, Raymond, Douglas S, Lee, Roberto A, Calle, Atalanta, Ghosh, Peter J, Savage, Sudha S, Shankar, Maria T, Vassileva, Gordon C, Weir, David A, Fryburg, and Krystyna, Tatarkiewicz
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Arginine ,Physiology ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Stimulation ,Type 2 diabetes ,Glucagon ,Body Mass Index ,chemistry.chemical_compound ,Physiology (medical) ,Internal medicine ,Insulin-Secreting Cells ,Insulin Secretion ,Medicine ,Humans ,Insulin ,Obesity ,Paresthesia ,Infusions, Intravenous ,Aged ,C-Peptide ,business.industry ,C-peptide ,Mouth Mucosa ,Reproducibility of Results ,Nausea ,Middle Aged ,medicine.disease ,Kinetics ,Clinical research ,Endocrinology ,chemistry ,Hyperglycemia ,Innovative Methodology ,Female ,business ,Function (biology) - Abstract
A key aspect of research into the prevention and treatment of type 2 diabetes is the availability of reproducible clinical research methodology to assess β-cell function. One commonly used method employs nonglycemic secretagogues like arginine (arg) or glucagon (glgn). This study was designed to quantify the insulin response to arg and glgn and determine test repeatability and tolerability. Obese overnight-fasted subjects with normal glucose tolerance were studied on 4 separate days: twice using arg (5 g iv) and twice with glgn (1 mg iv). Pre- and postinfusion samples for plasma glucose, insulin, and C-peptide were acquired. Arg and glgn challenges were repeated in the last 10 min of a 60-min glucose (900 mg/min) infusion. Insulin and C-peptide secretory responses were estimated under baseline fasting glucose conditions (AIRarg and AIRglgn) and hyperglycemic (AIRargMAX AIRglgnMAX) states. Relative repeatability was estimated by intraclass correlation coefficient (ICC). Twenty-three (12 men and 11 women) subjects were studied (age: 42.4 ± 8.3 yr; BMI: 31.4 ± 2.8 kg/m2). Geometric means (95% CI) for baseline-adjusted values AIRarg and AIRglgn were 84 (75–95) and 102 (90–115) μU/ml, respectively. After the glucose infusion, AIRargMAX and AIRglgnMAX were 395 (335–466) and 483 (355–658) μU/ml, respectively. ICC values were >0.90 for AIRarg andAIRargMAX. Glucagon ICCs were 0.83, 0.34, and 0.36, respectively, although the exclusion of one outlier increased the latter two values (to 0.84 and 0.86). Both glgn and arg induced mild adverse events that were transient. Glucagon, but not arginine, induced moderate adverse events due to nausea. Taken together, arginine is preferred to glucagon for assessment of β-cell function.
- Published
- 2014
17. [Untitled]
- Author
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Atalanta Ghosh, Mary Pat Knadler, Sandra R. B. Allerheiligen, Jeffrey W. Miller, and Andrej Skerjanec
- Subjects
Pharmacology ,medicine.medical_specialty ,business.industry ,Organic Chemistry ,Warfarin ,Pharmaceutical Science ,Lipid metabolism ,Drug interaction ,Endometrium ,Antiestrogen ,medicine.anatomical_structure ,Endocrinology ,Pharmacokinetics ,Selective estrogen receptor modulator ,Internal medicine ,medicine ,Molecular Medicine ,Pharmacology (medical) ,Raloxifene ,business ,hormones, hormone substitutes, and hormone antagonists ,Biotechnology ,medicine.drug - Abstract
Purpose. Evista® (raloxifene HCl) is a nonsteroidal selective estrogen receptor modulator that displays estrogen agonist effects on bone and lipid metabolism but estrogen antagonist effects on the breast and endometrium. The potential for drug-drug interaction between raloxifene and warfarin was assessed in 15 healthy postmenopausal women.
- Published
- 2001
18. Noninformative priors for one-parameter item response models
- Author
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Atalanta Ghosh, Ming-Hui Chen, Malay Ghosh, and Alan Agresti
- Subjects
Statistics and Probability ,Rasch model ,Applied Mathematics ,Bayesian probability ,Markov chain Monte Carlo ,Logistic regression ,Statistics::Computation ,symbols.namesake ,Distribution (mathematics) ,Probit model ,Statistics ,Prior probability ,symbols ,Statistics, Probability and Uncertainty ,Gibbs sampling ,Mathematics - Abstract
We present a unified Bayesian approach for the analysis of one-parameter item response models. A necessary and sufficient condition is given for the propriety of posteriors under improper priors with nonidentifiable likelihoods. Posterior distributions for item and subject parameters may be improper when the sum of the binary responses for an item or subject takes its minimum or maximum possible value. When the item parameters have a flat prior but the item totals do not fall at a boundary value, we prove the propriety of the Bayesian joint posterior under some sufficient conditions on the joint (proper) distribution of the subject parameters. The methods are implemented using Markov chain Monte Carlo and illustrated with an example from a cross-over study comparing three medical treatments.
- Published
- 2000
19. [Untitled]
- Author
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Benito J. Cerimele, Atalanta Ghosh, Daniel C. Howey, Karl A. DeSante, and Rodney P. Basson
- Subjects
Pharmacology ,Chemistry ,digestive, oral, and skin physiology ,Organic Chemistry ,Cmax ,Pharmaceutical Science ,Absorption (skin) ,Bioequivalence ,Dosage form ,Bioavailability ,Absorption rate ,Drug concentration ,Pharmacokinetics ,Statistics ,polycyclic compounds ,Molecular Medicine ,heterocyclic compounds ,Pharmacology (medical) ,human activities ,therapeutics ,Biotechnology - Abstract
Purpose. Peak drug concentration (Cmax) measures the extremity of drug exposure and is a secondary indicator of the extent of absorption after area under the concentration time curve (AUC). Cmax serves as the indicator of absorption rate in bioequivalence (BE) studies in the US (1). The use of Cmax, not the time to Cmax(Tmax), as the metric to assess absorption rate causes erratic inferences in BE studies, and incorrect conclusions for some. We can improve BE efficiency (i.e., get the answer right the first time), by properly analyzing the time to Cmax(Tmax) instead of Cmax.
- Published
- 1998
20. Raking Kappa: Describing Potential Impact of Marginal Distributions on Measures of Agreement
- Author
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Atalanta Ghosh, Matilde Bini, and Alan Agresti
- Subjects
Statistics and Probability ,Contingency table ,Potential impact ,Inter-rater reliability ,Cohen's kappa ,Statistics ,Econometrics ,General Medicine ,Statistics, Probability and Uncertainty ,Marginal distribution ,Kappa number ,Kappa ,Mathematics - Abstract
Several authors have noted the dependence of kappa measures of inter-rater agreement on the marginal distributions of contingency tables displaying the joint ratings. This paper introduces a smoothed version of kappa computed after raking the table to achieve pre-specified marginal distributions. A comparison of kappa with raked kappa for various margins can indicate the extent of the dependence on the margins, and can indicate how much of the lack of agreement is due to marginal heterogeneity.
- Published
- 1995
21. Phase 0 study of the inhibition of cholesteryl ester transfer protein activity by JNJ-28545595 in plasma from normolipidemic and dyslipidemic humans
- Author
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Bruce P. Damiano, Margery A. Connelly, Prasarn Manitpisitkul, Paul Rothenberg, Atalanta Ghosh, Elizabeth T. Leary, Keith T. Demarest, Troy C. Sarich, and Dorota B. Schranz
- Subjects
Adult ,Male ,Pharmacology ,In Vitro Techniques ,chemistry.chemical_compound ,Inhibitory Concentration 50 ,Cholesterylester transfer protein ,Humans ,Pharmacology (medical) ,In patient ,CETP inhibitor ,Triglycerides ,Aged ,Dyslipidemias ,Plasma samples ,biology ,Triglyceride ,Dose-Response Relationship, Drug ,Chemistry ,Cetp inhibition ,Middle Aged ,Lipids ,Cholesterol Ester Transfer Proteins ,carbohydrates (lipids) ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Biological Assay ,Female - Abstract
OBJECTIVE To assess and validate the application of a non-radioactive assay for cholesteryl ester transfer protein (CETP) activity in clinical samples. DESIGN AND METHODS In this Phase 0 study, CETP activity was measured following addition of the CETP inhibitor JNJ-28545595 to plasma samples from normolipidemic and three subgroups of dyslipidemic subjects with differing lipid profiles. RESULTS CETP activity was elevated in plasma samples from dyslipidemic subjects compared to normolipidemic subjects. Increased triglyceride levels correlated with decreased CETP inhibition. The assay was found to have good analytical precision and high throughput potential as required for clinical trial sample analysis. CONCLUSIONS The results demonstrate that pharmacological inhibition of CETP is affected by the dyslipidemic nature of plasma samples. In addition, since the optimal degree of CETP inhibition for maximal cardiovascular benefit in patients is not known, this assay may be used to help define optimal dosing of CETP inhibitors.
- Published
- 2012
22. Clinical application of a semimechanistic-physiologic population PK/PD model for neutropenia following pemetrexed therapy
- Author
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Atalanta Ghosh, Robert Johnson, Jane E. Latz, Mats O. Karlsson, and James J. Rusthoven
- Subjects
Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Antimetabolites, Antineoplastic ,Guanine ,Neutropenia ,Neutrophils ,Population ,Pemetrexed ,Pharmacology ,Toxicology ,Leukocyte Count ,Clinical Trials, Phase II as Topic ,Glutamates ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,Dosing ,education ,PK/PD models ,Aged ,education.field_of_study ,Models, Statistical ,Dose-Response Relationship, Drug ,business.industry ,Avitaminosis ,Middle Aged ,medicine.disease ,Clinical trial ,Pharmacodynamics ,Area Under Curve ,Absolute neutrophil count ,Female ,business ,Biomarkers ,medicine.drug - Abstract
Purpose: The objective of these analyses was to examine the effect of variations in the explanatory factors of neutropenic response, identified by semimechanistic-physiologic population pharmacokinetic/pharmacodynamic (PK/PD) modeling, on clinically important features of the absolute neutrophil count (ANC)-time profile (e.g, the nadir of the ANC [NANC], its timing [TNadir], and the timecourse of recovery [TRec]). Methods: Correlation analyses were used to evaluate the relationship of NANC, TNadir, and TRec as a function of overall systemic exposure (AUC) and each of the covariates contained in the population PK/PD model. Simulations using the final PK/PD model were used to generate complete ANC-time profiles. Frequency counts of NANCs from the simulated profiles were used to quantitatively explore differences in the incidence and severity of neutropenia associated with a variety of scenarios (500 mg/m2 versus 600 mg/m2, normal vitamin deficiency markers versus elevated vitamin deficiency markers, and body surface area-based versus renal function-based dosing) and to evaluate the effect of individual explanatory factors with respect to neutropenic response. Results: Information obtained from correlation analysis and simulations was helpful in quantitatively exploring the impact of dose, exposure, and/or patient characteristics on neutropenic response. The information gained from these simulations provided supportive evidence for the decision to routinely include vitamin supplementation during pemetrexed treatment as a means of managing the risk of severe neutropenia secondary to pemetrexed administration. These techniques also provided information regarding the specific TNadir and TRec for inclusion in product labeling and suggested that a 14-day treatment cycle might be feasible for pemetrexed. Conclusion: For population PK/PD models, to provide useful information for the practicing clinician or the clinical development team, it is not sufficient to look only at influences of covariates on model parameters. Rather, the modeling results need to be carefully investigated in terms of clinically relevant measures.
- Published
- 2004
23. A semimechanistic-physiologic population pharmacokinetic/pharmacodynamic model for neutropenia following pemetrexed therapy
- Author
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Atalanta Ghosh, Jane E. Latz, Robert Johnson, Mats O. Karlsson, and James J. Rusthoven
- Subjects
Oncology ,Male ,Cancer Research ,Homocysteine ,Body Surface Area ,Neutrophils ,Pharmacology ,Toxicology ,chemistry.chemical_compound ,Leukocyte Count ,Glutamates ,Medicine ,Pharmacology (medical) ,Infusions, Intravenous ,education.field_of_study ,Vitamins ,Middle Aged ,Vitamin B 12 ,Pemetrexed ,Area Under Curve ,Data Interpretation, Statistical ,Antifolate ,Absolute neutrophil count ,Female ,Algorithms ,medicine.drug ,Adult ,medicine.medical_specialty ,Antimetabolites, Antineoplastic ,Guanine ,Neutropenia ,Population ,Clinical Trials, Phase II as Topic ,Folic Acid ,Internal medicine ,Humans ,Vitamin B12 ,education ,Aged ,Analysis of Variance ,Models, Statistical ,business.industry ,Avitaminosis ,medicine.disease ,Vitamin B 6 ,Blood Cell Count ,chemistry ,Pharmacodynamics ,business - Abstract
Purpose: The objectives of these analyses were to (1) develop a semimechanistic-physiologic population pharmacokinetic/pharmacodynamic (PK/PD) model to describe neutropenic response to pemetrexed and to (2) identify influential covariates with respect to pharmacodynamic response. Patients and methods: Data from 279 patients who received 1,136 treatment cycles without folic acid or vitamin B12 supplementation during participation in one of eight phase II cancer trials were available for analysis. Starting doses were 500 or 600 mg pemetrexed per m2 body surface area (BSA), administered as 10-min intravenous infusions every 21 days (1 cycle). The primary analyses included 105 patients (279 cycles) for which selected covariates—including vitamin deficiency marker data (i.e., homocysteine, cystathionine, methylmalonic acid, and methylcitrate [I, II, and total] plasma concentrations)—were available. Classical statistical multivariate regression analyses and a semimechanistic-physiologic population PK/PD model were used to evaluate neutropenic response to single-agent pemetrexed administration. Results: The timecourse of neutropenia following single-agent pemetrexed administration was adequately described by a semimechanistic-physiologic model. Population estimates for system-based model parameters (i.e., baseline neutrophil count, mean transit time, and the feedback parameter), which mathematically represent current understanding of the process and physiology of hematopoiesis, were consistent with previously reported values. The population PK/PD model included homocysteine, cystathionine, albumin, total protein, and BSA as covariates relative to neutropenic response. Conclusion: These results support the programmatic decision to introduce folic acid and vitamin B12 supplementation during pemetrexed clinical development as a means of normalizing patient homocysteine levels, thereby managing the risk of severe neutropenia secondary to pemetrexed administration. The current results also suggest that the addition of vitamin B6 supplementation to normalize patient cystathionine levels may further decrease the incidence of grade 4 neutropenia following pemetrexed administration. The results also suggest the use of folic acid as a means of lessening hematologic toxicity following administration of cytotoxic agents other than antifolates.
- Published
- 2004
24. Pharmacodynamic Effects of Canagliflozin, a Sodium Glucose Co-Transporter 2 Inhibitor, from a Randomized Study in Patients with Type 2 Diabetes
- Author
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Linda Morrow, Keith T. Demarest, David Polidori, Paul Rothenberg, Heike Spitzer, Sabine Arnolds, Sue Sha, Damayanthi Devineni, Atalanta Ghosh, and Marcus Hompesch
- Subjects
Blood Glucose ,Male ,medicine.medical_treatment ,lcsh:Medicine ,Type 2 diabetes ,Kidney ,Endocrinology ,Glucosides ,Medicine and Health Sciences ,Insulin ,lcsh:Science ,Canagliflozin ,Multidisciplinary ,Fasting ,Middle Aged ,Tolerability ,Research Design ,Female ,medicine.symptom ,Research Article ,Glomerular Filtration Rate ,medicine.drug ,Adult ,Glycosuria ,medicine.medical_specialty ,Clinical Research Design ,Oral Medicine ,Urology ,Thiophenes ,Urinalysis ,Hypoglycemia ,Research and Analysis Methods ,Drug Administration Schedule ,Double-Blind Method ,Sodium-Glucose Transporter 2 ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Hypoglycemic Agents ,Sodium-Glucose Transporter 2 Inhibitors ,Aged ,Dose-Response Relationship, Drug ,business.industry ,Body Weight ,lcsh:R ,Correction ,medicine.disease ,Diabetes Mellitus, Type 2 ,Metabolic Disorders ,lcsh:Q ,Renal threshold ,business - Abstract
Introduction This randomized, double-blind, placebo-controlled, single and multiple ascending-dose study evaluated the pharmacodynamic effects and safety/tolerability of canagliflozin, a sodium glucose co-transporter 2 inhibitor, in patients with type 2 diabetes. Methods Patients (N = 116) discontinued their antihyperglycemic medications 2 weeks before randomization. Patients received canagliflozin 30, 100, 200, or 400 mg once daily or 300 mg twice daily, or placebo at 2 study centers in the United States and Germany, or canagliflozin 30 mg once daily or placebo at 1 study center in Korea, while maintaining an isocaloric diet for 2 weeks. On Days –1, 1, and 16, urinary glucose excretion (UGE), plasma glucose (PG), fasting PG (FPG), and insulin were measured. The renal threshold for glucose (RTG) was calculated from UGE, PG, and estimated glomerular filtration rate. Safety was evaluated based on adverse event (AE) reports, vital signs, electrocardiograms, clinical laboratory tests, and physical examinations. Results Canagliflozin increased UGE dose-dependently (∼80–120 g/day with canagliflozin ≥100 mg), with increases maintained over the 14-day dosing period with each dose. Canagliflozin dose-dependently decreased RTG, with maximal reductions to ∼4–5 mM (72–90 mg/dL). Canagliflozin also reduced FPG and 24-hour mean PG; glucose reductions were seen on Day 1 and maintained over 2 weeks. Plasma insulin reductions with canagliflozin were consistent with observed PG reductions. Canagliflozin also reduced body weight. AEs were transient, mild to moderate in intensity, and balanced across groups; 1 canagliflozin-treated female reported an episode of vaginal candidiasis. Canagliflozin did not cause hypoglycemia, consistent with the RTG values remaining above the hypoglycemia threshold. At Day 16, there were no clinically meaningful changes in urine volume, urine electrolyte excretion, renal function, or routine laboratory test values. Conclusions Canagliflozin increased UGE and decreased RTG, leading to reductions in PG, insulin, and body weight, and was generally well tolerated in patients with type 2 diabetes. Trial Registration ClinicalTrials.gov NCT00963768
- Published
- 2014
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