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4. Opsonization-independent antigen-specific recognition by myeloid phagocytes expressing monoclonal antibodies

Author

Neumaier, Michael, primary, Giesler, Sophie, additional, Ast, Volker, additional, Roemer, Mathis, additional, Voß, Timo-Daniel, additional, Reinz, Eileen, additional, Costina, Victor, additional, Schmelz, Martin, additional, Nürnberg, Elina, additional, Nittka, Stefanie, additional, Leppä, Aino-Maija, additional, Rudolf, Ruediger, additional, Trumpp, Andreas, additional, and Fuchs, Tina, additional

Published
2023
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6. Exploring the Synergistic Potential of Radiomics and Laboratory Biomarkers for Enhanced Identification of Vulnerable COVID-19 Patients

Author

Gerhards, Catharina, primary, Haselmann, Verena, additional, Schaible, Samuel F., additional, Ast, Volker, additional, Kittel, Maximilian, additional, Thiel, Manfred, additional, Hertel, Alexander, additional, Schoenberg, Stefan O., additional, Neumaier, Michael, additional, and Froelich, Matthias F., additional

Published
2023
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9. Epigenetic signatures of cfDNA enable a topological assignment of tissue damage.

Author

Gerhards, Catharina, Evliyaoglu, Osman, Ast, Volker, and Neumaier, Michael

Subjects
CELL-free DNA, EPIGENETICS, COVID-19, PROSTATE, PROSTATE cancer, TISSUES, ENDORECTAL ultrasonography
Abstract

This article discusses the potential use of epigenetic signatures of cell-free DNA (cfDNA) as biomarkers for identifying tissue damage. The authors analyzed the epigenetic signatures of cfDNA in COVID-19 patients and found that varying proportions of releasing cells and involvement of other organ systems could differentiate between severe and milder cases of the disease. They also presented a case study of a patient with a significantly elevated prostate signal in their cfDNA, which could potentially aid in diagnosing prostate cancer. The authors suggest that analyzing epigenetic patterns of cfDNA could be a cost-effective and minimally invasive method for screening and identifying cell damage. [Extracted from the article]

Published
2024
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10. Publisher Correction: Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly

Author

Irizar, Peer Aramillo, Schäuble, Sascha, Esser, Daniela, Groth, Marco, Frahm, Christiane, Priebe, Steffen, Baumgart, Mario, Hartmann, Nils, Marthandan, Shiva, Menzel, Uwe, Müller, Jule, Schmidt, Silvio, Ast, Volker, Caliebe, Amke, König, Rainer, Krawczak, Michael, Ristow, Michael, Schuster, Stefan, Cellerino, Alessandro, Diekmann, Stephan, Englert, Christoph, Hemmerich, Peter, Sühnel, Jürgen, Guthke, Reinhard, Witte, Otto W., Platzer, Matthias, Ruppin, Eytan, and Kaleta, Christoph

Published
2019
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12. Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly

Author

Aramillo Irizar, Peer, Schäuble, Sascha, Esser, Daniela, Groth, Marco, Frahm, Christiane, Priebe, Steffen, Baumgart, Mario, Hartmann, Nils, Marthandan, Shiva, Menzel, Uwe, Müller, Jule, Schmidt, Silvio, Ast, Volker, Caliebe, Amke, König, Rainer, Krawczak, Michael, Ristow, Michael, Schuster, Stefan, Cellerino, Alessandro, Diekmann, Stephan, Englert, Christoph, Hemmerich, Peter, Sühnel, Jürgen, Guthke, Reinhard, Witte, Otto W., Platzer, Matthias, Ruppin, Eytan, and Kaleta, Christoph

Published
2018
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13. Liquid profiling of circulating tumor DNA in colorectal cancer: steps needed to achieve its full clinical value as standard care

Author

Hedtke, Maren, primary, Pessoa Rejas, Rodrigo, additional, Froelich, Matthias F., additional, Ast, Volker, additional, Duda, Angelika, additional, Mirbach, Laura, additional, Costina, Victor, additional, Martens, Uwe M., additional, Hofheinz, Ralf‐Dieter, additional, Neumaier, Michael, additional, and Haselmann, Verena, additional

Published
2021
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14. Assessing the Quality of Serological Testing in the COVID-19 Pandemic: Results of a European External Quality Assessment (EQA) Scheme for Anti-SARS-CoV-2 Antibody Detection

Author

Ast, Volker, primary, Costina, Victor, additional, Eichner, Romy, additional, Bode, Anna, additional, Aida, Sihem, additional, Gerhards, Catharina, additional, Thiaucourt, Margot, additional, Dobler, Gerhard, additional, Geilenkeuser, Wolf-Jochen, additional, Wölfel, Roman, additional, Neumaier, Michael, additional, and Haselmann, Verena, additional

Published
2021
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15. Liquid profiling of circulating tumor DNA in colorectal cancer: steps needed to achieve its full clinical value as standard care.

Author

Hedtke, Maren, Pessoa Rejas, Rodrigo, Froelich, Matthias F., Ast, Volker, Duda, Angelika, Mirbach, Laura, Costina, Victor, Martens, Uwe M., Hofheinz, Ralf-Dieter, Neumaier, Michael, and Haselmann, Verena

Abstract

The analysis of circulating tumor DNA (ctDNA) is at the threshold of implementation into standard care for colorectal cancer (CRC) patients. However, data about the clinical utility of liquid profiling (LP), its acceptance by clinicians, and its integration into clinical workflows in real-world settings remain limited. Here, LP tests requested as part of routine care since 2016 were retrospectively evaluated. Results show restrained request behavior that improved moderately over time, as well as reliable diagnostic performance comparable to translational studies, with an overall agreement of 91.7%. Extremely low ctDNA levels at < 0.1% in over 20% of cases, a high frequency of concomitant driver mutations (in up to 14% of cases), and ctDNA levels reflecting the clinical course of disease were revealed. However, certain limitations hampering successful translation of ctDNA into clinical practice were uncovered, including the lack of clinically relevant ctDNA thresholds, appropriate time points of LP requests, and integrative evaluation of ctDNA, imaging, and clinical findings. In conclusion, these results highlight the potential clinical value of LP for CRC patient management and demonstrate issues that need to be addressed for successful long-term implementation in clinical workflows. [ABSTRACT FROM AUTHOR]

Published
2022
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18. Dissecting the genomic complexity underlying medulloblastoma

Author

Jones, David T. W., Jäger, Natalie, Kool, Marcel, Zichner, Thomas, Hutter, Barbara, Sultan, Marc, Cho, Yoon-Jae, Pugh, Trevor J., Hovestadt, Volker, Stütz, Adrian M., Rausch, Tobias, Warnatz, Hans-Jörg, Ryzhova, Marina, Bender, Sebastian, Sturm, Dominik, Pleier, Sabrina, Cin, Huriye, Pfaff, Elke, Sieber, Laura, Wittmann, Andrea, Remke, Marc, Witt, Hendrik, Hutter, Sonja, Tzaridis, Theophilos, Weischenfeldt, Joachim, Raeder, Benjamin, Avci, Meryem, Amstislavskiy, Vyacheslav, Zapatka, Marc, Weber, Ursula D., Wang, Qi, Lasitschka, Bärbel, Bartholomae, Cynthia C., Schmidt, Manfred, von Kalle, Christof, Ast, Volker, Lawerenz, Chris, Eils, Jürgen, Kabbe, Rolf, Benes, Vladimir, van Sluis, Peter, Koster, Jan, Volckmann, Richard, Shih, David, Betts, Matthew J., Russell, Robert B., Coco, Simona, Tonini, Gian Paolo, Schüller, Ulrich, Hans, Volkmar, Graf, Norbert, Kim, Yoo-Jin, Monoranu, Camelia, Roggendorf, Wolfgang, Unterberg, Andreas, Herold-Mende, Christel, Milde, Till, Kulozik, Andreas E., von Deimling, Andreas, Witt, Olaf, Maass, Eberhard, Rössler, Jochen, Ebinger, Martin, Schuhmann, Martin U., Frühwald, Michael C., Hasselblatt, Martin, Jabado, Nada, Rutkowski, Stefan, von Bueren, André O., Williamson, Dan, Clifford, Steven C., McCabe, Martin G., Collins, Peter V., Wolf, Stephan, Wiemann, Stefan, Lehrach, Hans, Brors, Benedikt, Scheurlen, Wolfram, Felsberg, Jörg, Reifenberger, Guido, Northcott, Paul A., Taylor, Michael D., Meyerson, Matthew, Pomeroy, Scott L., Yaspo, Marie-Laure, Korbel, Jan O., Korshunov, Andrey, Eils, Roland, Pfister, Stefan M., and Lichter, Peter

Published
2012
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20. Functional surface proteomic profiling reveals the host heat‐shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages

Author

Hassan, Mohamed I. Abdelwahab, primary, Kruse, Janis M., additional, Krüger, Thomas, additional, Dahse, Hans‐Martin, additional, Cseresnyés, Zoltán, additional, Blango, Matthew G., additional, Slevogt, Hortense, additional, Hörhold, Franziska, additional, Ast, Volker, additional, König, Rainer, additional, Figge, Marc Thilo, additional, Kniemeyer, Olaf, additional, Brakhage, Axel A., additional, and Voigt, Kerstin, additional

Published
2020
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21. Lack of CD45 in FLT3-ITD mice results in a myeloproliferative phenotype, cortical porosity, and ectopic bone formation

Author

Kresinsky, Anne, primary, Schnöder, Tina M., additional, Jacobsen, Ilse D., additional, Rauner, Martina, additional, Hofbauer, Lorenz C., additional, Ast, Volker, additional, König, Rainer, additional, Hoffmann, Bianca, additional, Svensson, Carl-Magnus, additional, Figge, Marc Thilo, additional, Hilger, Ingrid, additional, Heidel, Florian H., additional, Böhmer, Frank- D., additional, and Müller, Jörg P., additional

Published
2019
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25. Additional file 1: Table S1. of SOX5 is involved in balanced MITF regulation in human melanoma cells

Author

Kordaß, Theresa, Weber, Claudia, Oswald, Marcus, Ast, Volker, Bernhardt, Mathias, Novak, Daniel, Utikal, Jochen, Eichmüller, Stefan, and König, Rainer

Abstract

Correlation of activity parameter for all putative MITF TFs with MITF expression levels for the NCI-60 panel. Table S2. Comparison of expression levels of SOX5 to different SOX family members (from the SKCM samples). Figure S1. Shows density plot of TBA z-scores for all TFs and target genes. Figure S2. Shows map of the used lentiviral vector MITFP-pLenti. Figure S3. Validation of the functionality of the melanocyte-specific lentiviral reporter vector MITFP-pLenti by comparing melanocytes and fibroblasts. Figure S4. Hierarchical cluster analysis of the NCI-60 cell line panel based on SOX10, SOX5 and MITF expression. Figure S5. Validation of siRNA mediated knock-down of SOX5 by qRT-PCR. Figure S6. Results of cell viability assay after transfection with SOX5 siRNA pool for five melanoma cell lines. Figure S7. Results of invasion assay after transfection with SOX5 siRNA pool. Figure S8. Boxplot SOX5 expression for primary and distant metastasis samples. Figure S9. Histogram of SOX5 expression for SCKM samples with vital status dead. Figure S10. Bimodal distribution of SOX5 expression for thick subgroup of the SKCM samples with Breslow thickness > 4 mm. Figure S11. Density and histogram of SOX5 expression for melanoma samples from the 33 melanoma cell lines. Figure S12. Kaplan-Meier analysis of SKCM samples based on MITF expression. (DOCX 1173 kb)

Published
2016
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28. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin

Author

Costa, Barbara, primary, Kean, Michelle J., additional, Ast, Volker, additional, Knight, James D.R., additional, Mett, Alice, additional, Levy, Zehava, additional, Ceccarelli, Derek F., additional, Badillo, Beatriz Gonzalez, additional, Eils, Roland, additional, König, Rainer, additional, Gingras, Anne-Claude, additional, and Fainzilber, Mike, additional

Published
2012
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29. Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly

Author

Julia J. Müller, Michael Ristow, Eytan Ruppin, Amke Caliebe, Steffen Priebe, Stefan Schuster, Shiva Marthandan, Alessandro Cellerino, Matthias Platzer, Peer Aramillo Irizar, Silvio Schmidt, Christoph Englert, Nils Hartmann, Rainer König, Jürgen Sühnel, Stephan Diekmann, Sascha Schäuble, Uwe Menzel, Michael Krawczak, Otto W. Witte, Peter Hemmerich, Marco Groth, Volker Ast, Reinhard Guthke, Christiane Frahm, Christoph Kaleta, Daniela Esser, Mario Baumgart, Aramillo Irizar, Peer, Schäuble, Sascha, Esser, Daniela, Groth, Marco, Frahm, Christiane, Priebe, Steffen, Baumgart, Mario, Hartmann, Nil, Marthandan, Shiva, Menzel, Uwe, Müller, Julia, Schmidt, Silvio, Ast, Volker, Caliebe, Amke, König, Rainer, Krawczak, Michael, Ristow, Michael, Schuster, Stefan, Cellerino, Alessandro, Diekmann, Stephan, Englert, Christoph, Hemmerich, Peter, Sühnel, Jürgen, Guthke, Reinhard, Witte, Otto W, Platzer, Matthia, Ruppin, Eytan, and Kaleta, Christoph

Subjects
0301 basic medicine, Aging, General Physics and Astronomy, Bioinformatics, Settore BIO/09 - Fisiologia, Transcriptome, Mice, Diabetes mellitus genetics, Fundulidae, Neoplasms, Epidemiology, Child, Zebrafish, Skin, Cancer, Aged, 80 and over, Multidisciplinary, Brain, Neurodegenerative Diseases, Middle Aged, Publisher Correction, Liver, Cardiovascular Diseases, Child, Preschool, Adult, medicine.medical_specialty, Adolescent, Degenerative Disorder, Science, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Computational biology and bioinformatics, Genetic association study, Ageing, Diabetes Mellitus, medicine, Animals, Humans, Aged, Genome, Human, Infant, Molecular Sequence Annotation, General Chemistry, medicine.disease, Chronic disorders, Gene Ontology, 030104 developmental biology, Chronic Disease, Risk allele
Abstract

Disease epidemiology during ageing shows a transition from cancer to degenerative chronic disorders as dominant contributors to mortality in the old. Nevertheless, it has remained unclear to what extent molecular signatures of ageing reflect this phenomenon. Here we report on the identification of a conserved transcriptomic signature of ageing based on gene expression data from four vertebrate species across four tissues. We find that ageing-associated transcriptomic changes follow trajectories similar to the transcriptional alterations observed in degenerative ageing diseases but are in opposite direction to the transcriptomic alterations observed in cancer. We confirm the existence of a similar antagonism on the genomic level, where a majority of shared risk alleles which increase the risk of cancer decrease the risk of chronic degenerative disorders and vice versa. These results reveal a fundamental trade-off between cancer and degenerative ageing diseases that sheds light on the pronounced shift in their epidemiology during ageing., Nature Communications, 9, ISSN:2041-1723

Published
2018

30. S100A9 and HMGB1 orchestrate MDSC-mediated immunosuppression in melanoma through TLR4 signaling.

Author

Özbay Kurt FG, Cicortas BA, Balzasch BM, De la Torre C, Ast V, Tavukcuoglu E, Ak C, Wohlfeil SA, Cerwenka A, Utikal J, and Umansky V

Subjects
Humans, Male, Female, Tumor Microenvironment immunology, Middle Aged, Immune Tolerance, Calgranulin B metabolism, Toll-Like Receptor 4 metabolism, HMGB1 Protein metabolism, Melanoma immunology, Melanoma metabolism, Melanoma drug therapy, Myeloid-Derived Suppressor Cells metabolism, Myeloid-Derived Suppressor Cells immunology, Signal Transduction
Abstract

Background: Immunotherapies for malignant melanoma are challenged by the resistance developed in a significant proportion of patients. Myeloid-derived suppressor cells (MDSC), with their ability to inhibit antitumor T-cell responses, are a major contributor to immunosuppression and resistance to immune checkpoint therapies in melanoma. Damage-associated molecular patterns S100A8, S100A9, and HMGB1, acting as toll like receptor 4 (TLR4) and receptor for advanced glycation endproducts (RAGE) ligands, are highly expressed in the tumor microenvironment and drive MDSC activation. However, the role of TLR4 and RAGE signaling in the acquisition of MDSC immunosuppressive properties remains to be better defined. Our study investigates how the signaling via TLR4 and RAGE as well as their ligands S100A9 and HMGB1, shape MDSC-mediated immunosuppression in melanoma., Methods: MDSC were isolated from the peripheral blood of patients with advanced melanoma or generated in vitro from healthy donor-derived monocytes. Monocytes were treated with S100A9 or HMGB1 for 72 hours. The immunosuppressive capacity of treated monocytes was assessed in the inhibition of T-cell proliferation assay in the presence or absence of TLR4 and RAGE inhibitors. Plasma levels of S100A8/9 and HMGB1 were quantified by ELISA. Single-cell RNA sequencing (scRNA-seq) was performed on monocytes from patients with melanoma and healthy donors., Results: We showed that exposure to S100A9 and HMGB1 converted healthy donor-derived monocytes into MDSC through TLR4 signaling. Our scRNA-seq data revealed in patient monocytes enriched inflammatory genes, including S100 and those involved in NF-κB and TLR4 signaling, and a reduced major histocompatibility complex II gene expression. Furthermore, elevated plasma S100A8/9 levels correlated with shorter progression-free survival in patients with melanoma., Conclusions: These findings highlight the critical role of TLR4 and, to a lesser extent, RAGE signaling in the conversion of monocytes into MDSC-like cells, underscore the potential of targeting S100A9 to prevent this conversion, and highlight the prognostic value of S100A8/9 as a plasma biomarker in melanoma., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Published
2024
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