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Electric fences are widely used in human–elephant conflict mitigation. However, elephants constantly challenge and overcome fences, which is a growing concern. We studied how elephants crossed the perimeter electric fence around the Udawalawe National Park (UWNP), Sri Lanka. Using camera traps, we monitored 18 fence posts and an open gate in the fence over 605 days from 2016 to 2019. Photographs of 3097 fence crossings were analysed to obtain demographic data for fence crossing elephants, crossing methods, and the daily and seasonal timing of fence crossing. We identified 77 adult males and 12 adult females that crossed the fence, which represent about 17% of the adult elephants in UWNP. Most of the crossings were through the open gate at night. Elephants used a variety of crossing methods, comprised of crossing at the open gate, at previously toppled posts, by crashing through wires and stepping over wires, as well as by using the trunk or forefeet to topple posts. Fence voltage was not a determinant of fence crossing. The frequency and timing of fence crossings varied between locations with later exits and less time spent outside, at crossing points bordering human dominated areas. Our results emphasis on the importance of taking into account the problem solving abilities of elephants in fence design and location, such as energising fence posts and constructing exclosure fences at the boundaries of crop fields and settlements, in employing electric fences to mitigate human‐elephant conflict. [ABSTRACT FROM AUTHOR]

Abstract Large mammals with general habitat needs can persist throughout mixed used landscapes, however, human-wildlife conflict frequently leads to their restriction to protected areas. Conservation efforts, especially for reducing conflicts with humans, can enhance tolerance of humans towards species like Asian elephants (Elephas maximus) in human-dominated landscapes. Here, we examine how elephant use in the Chure Terai Madhesh Landscape (CTML) covering the entire elephant range of Nepal changed between 2012 and 2020 in relationship to protection status and environmental conditions. We systematically surveyed ~ 42,000 km2 of potential habitat, by dividing the study area into 159 grid cells of 15 × 15 km2 and recorded elephant signs during the cool, dry season in three years (2012, 2018 and 2020). We analyzed the survey data in a single-species, multi-season (dynamic) occupancy modeling framework to test hypotheses regarding the influence of environmental conditions and protected area status on landscape use by elephants over time. The best-supported model included protected area effects on initial use, colonization, and detection probability as well as temporal variation in colonization and detection probability. Initial use and colonization rates were higher in protected areas, however elephants increasingly used cells located both inside and outside the protected areas, and the difference in use between protected areas and outside declined as elephants use became prevalent across most of the landscape. While elephant use was patchily distributed in the first year of surveys consistent with past descriptions of four sub-populations, elephant use consolidated into a western and eastern region in subsequent years with a gap in their distribution occurring between Chitwan and Bardiya National Parks. Our manuscript highlights the increasing landscape use by elephants in both protected areas and areas outside protected areas and suggests that management interventions that focus on reducing conflicts can promote greater use of both protected areas and areas outside of protected areas.

Aim: A study was methodized to evaluate whether supplementation of probiotics could impact the utilization of nutrients and energy in Asian elephants. Materials and Methods: Eighteen elephants were randomly selected for 60 days experiment including five days of the digestibility trial and divided into three groups with six elephants each i.e. control with no probiotic (T1) and the other two with probiotics Lactobacillus acidophilus in T2 and Saccharomyces cerevisiae in T3 groups at 1 × 109 CFU/gm concentrate per 50 kg body weight/ day orally. Results: The intakes of ether extract and total ash revealed significant whereas the digestibility coefficients of total ash and acid-insoluble ash exhibited a highly significant effect of treatment. However, other values of intakes and the digestibility coefficients of proximate principles and fibre fractions could not reveal any significant effect. Practical nutritional worth, plane of nutrition and energy balance profile were also found to be nonsignificant among the groups. Increasing trend of intake and the digestibility of energy; and the relative difference was associated with increased DM intake and NDF digestibility. The mean values for TDN%, NR, digestible DM and OM intakes and TDN intake were obtained to be higher for probiotic supplemented groups than the control group. Conclusion: It is concluded that probiotic supplementation could not exhibit any significant effect on nutrients and energy utilization in elephants. However, further research is needed to examine the effects of high doses, strain and viability of the probiotics.

A crucial aspect of successful conservation strategies is the identification of critical aspects of local habitats required for species preservation in a given region, such as salt licks. Tangkahan is adjacent to the Gunung Leuser National Park in North Sumatra, Indonesia. The park collaborates with the Indonesia Conservation Response Unit using captive Sumatran Elephants Elephas maximus sumatranus for forest patrols, mitigation of human-elephant negative interactions, public education, and ecotourism in the area. An initial study on the daily activities of captive Sumatran Elephants revealed their search for salt licks, which are essential for maintaining their optimal daily sodium intake. Information on salt licks in Tangkahan is limited and deserves further investigation. Ethical clearance is deemed unnecessary, as the research employs a non-invasive approach, exclusively observing the natural behaviors, and daily activities of elephants. The well-being of the elephants takes precedence over invasive technologies, with continuous monitoring ensuring their care throughout the research process. The study utilizes a descriptive-analytic methodology, tracking the daily movements of Sumatran Elephants to identify the locations of salt licks in the area. Four salt licks--Encepan-1, Encepan-2, Namo Cencen, and Hot Spring--were identified by participating in the elephants' territorial exploration. Although the salt licks were located adjacently, Encepan-1 was most frequently visited by the elephants. The salt licks were characterized as waterholes containing Na+ (Sodium ion) rich waters from springs. However, according to the atomic absorption spectrophotometry (AAS) method, the sodium concentration in these salt licks ranged 34-55 ppm, which is estimated to be insufficient for the physiological requirements of the elephants. Therefore, further investigations are needed to explore other complementary salt licks and the incidence of geophagy to support the mineral needs of Sumatran Elephants in the Tangkahan region. A crucial aspect of successful conservation strategies is the identification of critical aspects of local habitats required for species preservation in a given region, such as salt licks. Tangkahan is adjacent to the Gunung Leuser National Park in North Sumatra, Indonesia. The park collaborates with the Indonesia Conservation Response Unit using captive Sumatran Elephants Elephas maximus sumatranus for forest patrols, mitigation of human-elephant negative interactions, public education, and ecotourism in the area. An initial study on the daily activities of captive Sumatran Elephants revealed their search for salt licks, which are essential for maintaining their optimal daily sodium intake. Information on salt licks in Tangkahan is limited and deserves further investigation. Ethical clearance is deemed unnecessary, as the research employs a non-invasive approach, exclusively observing the natural behaviors, and daily activities of elephants. The well-being of the elephants takes precedence over invasive technologies, with continuous monitoring ensuring their care throughout the research process. The study utilizes a descriptive-analytic methodology, tracking the daily movements of Sumatran Elephants to identify the locations of salt licks in the area. Four salt licks--Encepan-1, Encepan-2, Namo Cencen, and Hot Spring--were identified by participating in the elephants' territorial exploration. Although the salt licks were located adjacently, Encepan-1 was most frequently visited by the elephants. The salt licks were characterized as waterholes containing Na+ (Sodium ion) rich waters from springs. However, according to the atomic absorption spectrophotometry (AAS) method, the sodium concentration in these salt licks ranged 34-55 ppm, which is estimated to be insufficient for the physiological requirements of the elephants. Therefore, further investigations are needed to explore other complementary salt licks and the incidence of geophagy to support the mineral needs of Sumatran Elephants in the Tangkahan region. [ABSTRACT FROM AUTHOR]

Simple Summary: All elephant species are now endangered or critically endangered. It is extremely important for the veterinary care of elephants to be effective at diagnosing diseases and monitoring treatment. Routine blood cell testing is a standard method for monitoring elephant health and detecting possible infections. Elephants naturally have different ratios of white blood cells (WBCs; immune cells) in their blood compared to other animals, with unusually high percentages of one cell type (monocytes). Because elephant WBCs also look different from those of most other animals, a person must identify the cells under the microscope instead of using machine analysis. These elephant-specific WBC features may be important in fighting diseases, but very little is known about elephant monocytes. The aims of this study were to establish methods for isolating elephant monocytes, studying their function, and accurately identifying them using flow cytometry, a more precise technique than microscopy. Blood from Asian elephants was used. Methods were created and tested. An approach to identifying elephant monocytes using flow cytometry was established. These results will lead to better studies of the elephant immune system and can help to more accurately count WBCs in elephant samples. Optimal veterinary care of managed elephant populations is vital due to the continued decline of wild populations. Appropriate health monitoring and accurate disease diagnosis include hematologic evaluation. Elephant hematology is distinctive in that elephants have high percentages of monocytes in health. Elephant monocytes also have unusual morphology, a feature shared with manatees and rock hyraxes. Manual white blood cell counting is used for elephant hematology, as analyzers are generally inaccurate. The aims of this study were to evaluate basic cell isolation and functional testing protocols for use in elephant monocyte research, and to test several available antibodies via flow cytometry for use in elephant monocyte identification. Peripheral blood samples from five Asian elephants (Elephas maximus) were used. Methods for monocyte isolation and evaluation of phagocytic function were established. Putative lymphocyte and monocyte populations were identified using a scatter on flow cytometry. Antibodies against CD11b, CD11c, CD14, and ionized calcium-binding adapter molecule 1 (IBA1) were tested, with IBA1 showing the highest apparent diagnostic utility in labeling monocytes. Combined flow cytometric scatter and IBA1 positivity appear to identify Asian elephant monocytes. These data provide a methodologic basis for further investigation into elephant monocyte function and immune response to infection. [ABSTRACT FROM AUTHOR]

Context: The removal or supplementation of ejaculates with seminal plasma (SP) can affect cryotolerance and post-thaw survival of spermatozoa in many species. In the Asian elephant (Elephas maximus), elucidation of the SP proteome and investigation of how it affects spermatozoa may enable improvement of cryopreservation protocols. Aims: Herein, we characterise the Asian elephant SP proteome and investigate the impacts of SP on sperm cryotolerance in the presence of conspecific or heterospecific SP. Methods: Proteomic analysis of Asian elephant SP was performed using mass spectrometry on nine samples from three individuals. In a separate study, SP was removed from six ejaculates and spermatozoa were resuspended in Tris extender supplemented with: no seminal plasma (NOSP), conspecific SP from ejaculates exhibiting 'good' (GSP, >60%) or mixed sperm total motility (MSP), or horse SP (HSP). Samples underwent cryopreservation, and sperm parameters were compared prior to cryopreservation and after thawing (0 and 2 h). Key results: Mass spectrometry identified 155 proteins from an array of families. Significant differences were observed in post-thaw sperm quality between SP treatments: high concentrations of MSP (25%, v/v) displayed greater average path and straight-line velocity immediately after thawing (P < 0.05) and greater sperm motility index and beat cross frequency than NOSP after 2 h post-thaw incubation (P < 0.05). The addition of HSP improved sperm kinematic parameters compared to NOSP and GSP treatments (P < 0.05). Conclusions and implications: These preliminary findings suggest the potential of SP to enhance the cryosurvival of Asian elephant spermatozoa, with HSP showing particularly promising results compared to conspecific SP (GSP). Further research into the specific effects of Asian elephant SP proteins is warranted. There is an urgent need to develop a means of freezing Asian elephant semen, to enable captive breeding conservation programs to maintain genetic diversity. This study revealed important information about the proteins present in the seminal plasma of these animals and how this may contribute to the successful frozen storage of their spermatozoa. These findings will contribute to our understanding of differences in sperm quality between elephants and aid in developing sperm freezing protocols that will lead to the long-awaited birth of an Asian elephant calf from frozen spermatozoa. Photograph by Cameron Negus. This article belongs to the Collection Dedication to Jim Cummins. [ABSTRACT FROM AUTHOR]

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Background Approximately one-third of Asian elephants are managed under human care, participating in educational, cultural, religious, and tourist activities. Management conditions vary considerably among venues, raising questions about whether welfare needs are consistently being met, particularly for Asian tourist camp elephants. To evaluate the well-being of elephants engaged in tourist activities, an evidence-based tool is needed for routine assessments to identify potential welfare risks, aid in the development of better camp standards, and enable caretakers to address specific concerns. While many animal welfare tools exist, none have been designed to consider specific environments and management practices faced by elephants living and working in tourist camps. Methods Using direct observations and interviews, the Elephant Welfare Assessment Tool (EWAT) was developed for tourist camp elephants using the Five Domains Model as a framework. Measures were selected based on peer-reviewed literature, existing standards and guidelines, and opinions from animal welfare experts working with zoo and tourist camp elephants. The EWAT differs from existing tools by including criteria on work activities and restraint methods (e.g., chaining and ankus use), factors common in Asia but not often encountered by western zoo elephants. Measures were scored using a 0–2 Likert Scale. The tool was tested in Thailand and determined by calculating a content validity index (CVI) and conducting inter-rater and test-retest reliability tests. Results The initial tool included 18 animal-based and 21 resource-based measures across four domains: Nutrition (n = 5), Environment (n = 14), Health (n = 10), and Behavior and Mental State (n = 10). Index scores of content validity (CVI) (Item CVI (0.83), Scale CVI/Average (0.98), and Scale CVI/Universal (0.89)) were high. Measures scoring less than 0.83 were removed: the opportunity to mate, the mahout-elephant relationship, and mahout job satisfaction. The final tool consisted of 42 questions related to 36 measures, including 18 animal-based and 18 resource-based measures within the Nutrition (n = 5), Environment (n = 11), Health (n = 10), and Behavior and Mental State (n = 10) domains. Intraclass correlation coefficients (ICC) for inter-rater reliability (0.78–0.90, p < 0.05) and test-retest (0.77–0.91, p < 0.05) analyses conducted at two camps showed good agreement. Conclusions This new assessment tool (EWAT) is a context-specific, holistic method designed to offer a practical means of conducting individual and institutional-level assessments of elephant welfare in tourist camps. It is based on the Five Domains Model using reliable and validated animal- and resource-based measures, data collection through direct observation and interviews, and a numerical scoring system. The tool includes several criteria applicable to tourist rather than zoo venues to make it more relevant to the challenges faced by working elephants in Asia.

Introduction: Monitoring overnight behavior is important in assessing the overall wellbeing of ex-situ elephant populations, with recumbent rest and stereotypy as key indicators of welfare. However, there have been few studies that address the overnight behavior of singly housed male elephants with a history of stereotypy. We conducted an opportunistic case study of the overnight behavior (i.e., rest and stereotypy) of a singly housed geriatric male Asian elephant at the Oklahoma City Zoo to identify his overnight behavioral rhythms in response to physiological (musth) and environmental changes (habitat access, automatic timed feeders) and guide management strategies. Methods: Infrared cameras were utilized to conduct continuous focal-animal sampling of the elephant's behavior between 20:00 and 08:00 in indoor and outdoor habitats. Sampling occurred from January 2023 to October 2023, with a total of 179 overnight observations. Results: The elephant exhibited recumbent rest for 175.66 ± 6.80 (mean ± SE) minutes per night and was engaged in stereotypy for 175.88 ± 9.68 minutes per night. While there was no significant relationship between stereotypy and the sexual state of musth, musth was associated with reduced durations of recumbent rest. Access to both indoor and outdoor habitats was significantly related to decreased stereotypy compared to indoor-only treatments. Recumbent rest occurred most frequently between 02:00 and 05:00, and stereotypic behavior was most common between 06:00 and 08:00. Contrary to our expectations, automatic feeders did not significantly reduce stereotypy. Discussion: The results of this study provide insight into the behavioral patterns of a geriatric male Asian elephant, demonstrating the importance of implementing an animal-centered approach to enhance animal wellbeing. As the zoo-housed Asian elephant population grows and continues to age, this case study emphasizes the importance of developing comprehensive welfare strategies for the elephants in our care. [ABSTRACT FROM AUTHOR]

Simple Summary: Elephant endotheliotropic herpesvirus (EEHV) represents a significant viral infection in young Asian elephants (Elephas maximus). The EEHV1A genotype predominantly induces EEHV hemorrhagic disease, while the EEHV4 genotype also contributes to hemorrhagic symptoms affecting the gastrointestinal and cardiovascular systems, often leading to fatality in young Asian elephants. Recently, instances of co-infection involving EEHV1A and -4 in Asian elephants have been documented. The present study aims to further elucidate the clinical and pathological lesions in these EEHV1A and -4 co-infection cases, as well as refine treatment protocols. Three cases of double infection with elephant endotheliotropic herpesvirus (EEHV) types 1A and 4 in captive Asian elephants are presented. The first calf was a 4-year-old female that showed initial signs of lethargy and depression. The second calf was a 6-year-old female that displayed signs of depression and diarrhea and died within 48 h of the start of supportive treatment. The third was a 2-year-old male that died suddenly while living with the herd. Necropsies were performed in the first and second elephants, while only a tongue sample was collected from the third calf. EEHV infection was confirmed via quantitative PCR (qPCR) and gene sequencing, revealing double subtypes of EEHV1A and -4 infections. This study describes the hematological and pathological characteristics within the host following double EEHV infection. [ABSTRACT FROM AUTHOR]

Habitat restoration is an effective method for improving landscape connectivity, which can reduce habitat fragmentation. Maintaining landscape connectivity could promote connections between habitat, which is extremely essential to preserve gene flow and population viability. This study proposes a methodological framework to analyze landscape connectivity for Asian elephant habitat conservation, aiming to provide practical options for reducing habitat fragmentation and improving habitat connectivity. Our approach involved combining a species distribution model using MaxEnt and landscape functional connectivity models using graph theory to assess the impact on connectivity improvement via farmland/plantation restoration as habitat. The results showed that: (1) there were 119 suitable habitat patches of Asian elephant covering a total area of 1952.41 km2. (2) The connectivity between habitats improved significantly after vegetation restoration and the gain first decreased and then increased with the increase of dispersal distance. (3) The first few new habitat patches that were identified played an important role in improving connectivity, and the variation rate of connectivity gradually leveled off as the number of new habitats increased. (4) Prioritization of the 25 best new habitat patches increased connectivity from 0.54% to 5.59% as the dispersal distance increased and mainly was located between two Asian elephant distribution regions and two components. Establishment of new habitat patches was effective for improving or restoring connectivity. Our findings can be used as guidance for improving the studied fragmented Asian elephant habitats, and they can also be used as a reference for the habitat restoration of other endangered species heavily affected by habitat fragmentation. [ABSTRACT FROM AUTHOR]

The international travel ban instituted by the Thai government in March 2020 in response to the COVID-19 pandemic greatly affected how tourist camp elephants were managed, with reductions in exercise opportunities, longer chaining hours, and diminished food provisioning. This study was conducted to determine how those changes affected health and welfare biomarkers in individual elephants over the 2 years of the countrywide lockdown (April 2020-April 2022). Blood and fecal samples were collected from 58 elephants at six camps (monthly in Year 1, quarterly in Year 2) and analyzed for stress biomarkers -- fecal glucocorticoid metabolites (fGCM), serum oxidative stress [malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG)], and stress leukograms. Overall, fGCM concentrations increased within the first few months and remained higher than pre-COVID levels, as did the H/L ratio, a measure affected by cortisol. Serum 8-OHdG, an indicator of DNA oxidative damage, also increased over time, while monocytosis and lymphopenia further suggested alterations in immune function as a result of stress. By contrast, another marker of oxidative stress, serum MDA, declined, possibly in response to reduced roughage and supplement intake. A notable finding was a seasonal pattern of fGCM that was significantly different from previous studies. Whereas higher fGCM during the rainy season were observed in this study, previously, concentrations were highest during the winter, high tourist season. Thus, ironically, both the presence and absence of tourists have been associated with increased fGCM concentrations, albeit for different reasons. Camp management factors negatively affecting stress outcomes included shorter chain lengths, longer chain hours, lack of exercise, and reduced roughage and supplements. Overall, it was clear that camps struggled to maintain adequate care for elephants during the COVID-19 pandemic, highlighting the importance of tourist income and need for contingency plans to cope with potential future disruptions to tourism. [ABSTRACT FROM AUTHOR]

The western Terai Arc Landscape (wTAL) in Uttarakhand, India, marks the range limit for the Asian elephant in north‐western India. This region has been impacted by land‐use changes and infrastructure expansion for the last seven decades. To evaluate the impact of habitat deterioration on the population structure of elephants in the region, we characterized their genetic diversity and local genetic structure using mitochondrial (D loop) and nuclear DNA (microsatellites; n = 15) markers. We used tissue samples of 114 elephants from five different sub‐populations, collected between 2005 and 2014. The genetic variation was moderate (HO = 0.49–0.55) compared with other Indian elephant populations. Two mtDNA haplotypes were identified without strong spatial patterns across wTAL. Bayesian individual‐based clustering algorithm identified two genetic clusters (K = 2) with high admixture (50% at Q < 0.7) and no spatial adherence. Though K = 1 was not supported by the Bayesian algorithm, multivariate analysis and sibship patterns did not indicate genetic differentiation. The lack of spatial genetic structuring suggests high levels of gene flow, indicating that this population is still panmictic. This suggests that the life history traits of elephants as well as the ecological features of this landscape influence genetic connectivity. However, ongoing land use changes necessitate regular genetic monitoring in wTAL to identify incipient structuring caused by anthropogenic barriers to movement. [ABSTRACT FROM AUTHOR]

Tropical forests are undergoing land use change in many regions of the world, with an increasingly prominent trend of landscape fragmentation. Nevertheless, the Asian elephant (Elephas maximus) population in tropical forests of China has continued to increase, and their distribution range is expanding to human-modified landscapes, resulting in increased severe human-elephant conflicts. Little is known about the habitat adaptation strategies of Asian elephants in human-dominated habitats. Here, we applied maximum entropy modeling to extensive occurrence data (21,011 independent records) of Asian elephants in southwest China to explore temporal changes in their distribution under climate change. We also identified critical factors shaping their current distribution and habitat selection strategies concerning human-induced land-use changes at multiple spatial scales. We found that the area of suitable habitats for Asian elephants will slightly increase in the future (7,025 km2) compared with the present climatic scenario (6,468 km2). At a finer spatial scale, topographic variables such as elevation (74%) and slope (11%) are the dominant factors shaping the habitat selection of Asian elephants. However, at a larger spatial scale, anthropogenic variables are the main factors associated with the habitat preference of Asian elephants. Most suitable and sub-suitable habitats (65%) of Asian elephants overlap with human-dominated areas. Our findings suggest that the effects of human activity on Asian elephants are scale-dependent and demonstrate that the suitable habitats of Asian elephants in China are shifting to human-dominated regions. [ABSTRACT FROM AUTHOR]

Simple Summary: The conservation of endangered Asian elephants (especially when driven by those from non-range countries) is made more complex in cultures where 'owning' individuals for tourism practice is widely accepted. How can a wide variety of stakeholders find common ground upon which to build conservation plans amidst shifting social and environmental pressures? Endangered Asian elephants (Elephas maximus) find themselves at the center of debates involving politics, land use, human–wildlife conflict, and environmental justice. The intensity of such debates has led scholars to label conservation challenges as wicked problems with profound implications on local and global practice. In elephant range states such as Nepal, these debates are made more complex through human 'ownership' of endangered individuals for use in tourism, worship, or co-work. Human perspectives on the ethics of using animals for tourism are changing, even in areas heavily reliant on the tourism industry for survival. These debates become inflamed when non-residents take on adversarial positions despite an acceptance of the 'ownership' or 'use' of endangered individuals among local communities. Novel approaches are needed if there is any hope of establishing a common ground upon which to build relationships which may benefit community members, international interests, and endangered individuals. [ABSTRACT FROM AUTHOR]

In order to practice the concept of coordinated development of “Lucid Waters and Lush Mountains are Invaluable Assets” and “mountains, rivers, forests, farmlands, lakes, grasslands and deserts are part of the community of life”, this study takes “pursuing green development and promoting harmony between humanity and nature” proposed in the report of the 20th National Congress of the Communist Party of China as the theoretical guidance. With the goal of enhancing ecosystem integrity and connectivity, enhancing population communication, enhancing population viability, and providing a model for the construction of ecological corridors for flagship species, based on the current distribution and habitat selection strategies of Asian elephants, the study follows the technological process of “Obtaining species distribution—Identifying habitat sources and resistance surface relating to elephant distribution—Constructing corridors based on the least cost path model—Extracting corridor centrality, pinch points, and barrier through circuit theory—Field investigation and validation—Adjusting and optimizing corridor design—Recommending corridor construction design scheme—Carrying out long-term monitoring of wildlife utilization of corridors”, and suggests construction idea of Asian elephant ecological corridor and related conservation advices. Above conceptions and advice serve for the strategic layout of “intensifying the protection of ecosystems”, “improving the system of shields for ecological security, and developing corridors and biodiversity protection networks”, providing technical support for scientific protection and effective management of Asian elephant population, offering a scientific basis for habitat restoration and layout of planned national parks and other protected area. [ABSTRACT FROM AUTHOR]

IntroductionMonitoring overnight behavior is important in assessing the overall wellbeing of ex-situ elephant populations, with recumbent rest and stereotypy as key indicators of welfare. However, there have been few studies that address the overnight behavior of singly housed male elephants with a history of stereotypy. We conducted an opportunistic case study of the overnight behavior (i.e., rest and stereotypy) of a singly housed geriatric male Asian elephant at the Oklahoma City Zoo to identify his overnight behavioral rhythms in response to physiological (musth) and environmental changes (habitat access, automatic timed feeders) and guide management strategies.MethodsInfrared cameras were utilized to conduct continuous focal-animal sampling of the elephant’s behavior between 20:00 and 08:00 in indoor and outdoor habitats. Sampling occurred from January 2023 to October 2023, with a total of 179 overnight observations.ResultsThe elephant exhibited recumbent rest for 175.66 ± 6.80 (mean ± SE) minutes per night and was engaged in stereotypy for 175.88 ± 9.68 minutes per night. While there was no significant relationship between stereotypy and the sexual state of musth, musth was associated with reduced durations of recumbent rest. Access to both indoor and outdoor habitats was significantly related to decreased stereotypy compared to indoor-only treatments. Recumbent rest occurred most frequently between 02:00 and 05:00, and stereotypic behavior was most common between 06:00 and 08:00. Contrary to our expectations, automatic feeders did not significantly reduce stereotypy.DiscussionThe results of this study provide insight into the behavioral patterns of a geriatric male Asian elephant, demonstrating the importance of implementing an animal-centered approach to enhance animal wellbeing. As the zoo-housed Asian elephant population grows and continues to age, this case study emphasizes the importance of developing comprehensive welfare strategies for the elephants in our care.

Optimal veterinary care of managed elephant populations is vital due to the continued decline of wild populations. Appropriate health monitoring and accurate disease diagnosis include hematologic evaluation. Elephant hematology is distinctive in that elephants have high percentages of monocytes in health. Elephant monocytes also have unusual morphology, a feature shared with manatees and rock hyraxes. Manual white blood cell counting is used for elephant hematology, as analyzers are generally inaccurate. The aims of this study were to evaluate basic cell isolation and functional testing protocols for use in elephant monocyte research, and to test several available antibodies via flow cytometry for use in elephant monocyte identification. Peripheral blood samples from five Asian elephants (Elephas maximus) were used. Methods for monocyte isolation and evaluation of phagocytic function were established. Putative lymphocyte and monocyte populations were identified using a scatter on flow cytometry. Antibodies against CD11b, CD11c, CD14, and ionized calcium-binding adapter molecule 1 (IBA1) were tested, with IBA1 showing the highest apparent diagnostic utility in labeling monocytes. Combined flow cytometric scatter and IBA1 positivity appear to identify Asian elephant monocytes. These data provide a methodologic basis for further investigation into elephant monocyte function and immune response to infection.

Many protected areas worldwide have been established to protect the last natural refuges of flagship animal species. However, long-established protected areas do not always match the current distributions of target species under changing environmental conditions. Here we present a case study of the Asian elephant Elephas maximus in Xishuangbanna, south-west China, to evaluate whether the established protected areas match the species’ current distribution and to identify key habitat patches for Asian elephant conservation. Our results show that currently only 24.5% of the predicted Asian elephant distribution in Xishuangbanna is located within Xishuangbanna National Nature Reserve, which was established for elephant conservation. Based on the predicted Asian elephant distribution, we identified the most important habitat patches for elephant conservation in Xishuangbanna. The three most important patches were outside Xishuangbanna National Nature Reserve and together they contained 43.3% of the estimated food resources for Asian elephants in all patches in Xishuangbanna. Thus, we identified a spatial mismatch between immobile protected areas and mobile animals. We recommend the inclusion of the three identified key habitat patches in a new national park currently being planned by the Chinese authorities for the conservation of the Asian elephant.

Abstract The western Terai Arc Landscape (wTAL) in Uttarakhand, India, marks the range limit for the Asian elephant in north‐western India. This region has been impacted by land‐use changes and infrastructure expansion for the last seven decades. To evaluate the impact of habitat deterioration on the population structure of elephants in the region, we characterized their genetic diversity and local genetic structure using mitochondrial (D loop) and nuclear DNA (microsatellites; n = 15) markers. We used tissue samples of 114 elephants from five different sub‐populations, collected between 2005 and 2014. The genetic variation was moderate (HO = 0.49–0.55) compared with other Indian elephant populations. Two mtDNA haplotypes were identified without strong spatial patterns across wTAL. Bayesian individual‐based clustering algorithm identified two genetic clusters (K = 2) with high admixture (50% at Q

There are few studies on the changes of heart rate of the Asian elephant (Elephas maximus), one of the largest tropical terrestrial mammals, with its self-factors and external environment. By measuring the heart rate of 35 Asian elephants, ranging in age from 4 months to 52 years, using a non-invasive electrocardiogram sensor in their natural habitat at Wild Elephant Valley, Xishuangbanna of China, we found factors that significantly influenced the HR were season, phase of the day, age, body weight, and the interaction between some of the above factors. We also observed that Asian elephants had lower resting heart rate in the morning of hot season than the cold and mild season, and the differences were significant, but the heart rate increased to similar levels in the afternoon regardless of the season. HR also decreased with age in all seasons and phases of the day. However, there was no significant effect of sex. This study reveals the adaptability of Asian elephant to tropical environment, and provides a basic reference for heart rate of Asian elephant under various natural conditions.

With strict enforcement of the legal protection for Asian elephants (Elephas maximus) in China, the elephant population has steadily increased from 146 elephants in 1976 to over 300 elephants in 2023. More elephants occur in highly fragmented and human‐dominated landscapes, resulting in serious human–elephant conflicts (HEC). We investigated the temporal and spatial aspects of HEC in Xishuangbanna Prefecture, China, from 2011‒2015 and 2016‒2020. We analyzed the characteristics of crop raiding, property damage, and human injury and death by elephants. Then, we employed a multi‐model ensemble forecasting framework to perform a risk assessment, and compared the changes in HEC hotspots to explore the factors influencing conflict. Our data revealed that 91,311 HEC compensations were recorded from 2011‒2020 with a total compensation amount of 127.01 million yuan (17.40 million USD), 89.75% of which was crop compensation. Areas of risk in 2011‒2015 and 2016‒2020 were 2,505 km2 and 3,157 km2, respectively, with an increase in area of 26.01%. The HEC risk areas were mainly distributed in nature reserves and surrounding areas, and >65% of the risk areas were located in land‐use types dominated by artificial planting. Distance to farmland, distance to sparse wood, and slope had the greatest relative importance in the risk model evaluation. The mitigation measures that we recommend include strengthening the monitoring system for Asian elephants in areas with current and potential HEC risks; improving compensation mechanisms, such as determining accurate annual premiums, establishing a shared loss compensation mechanism, and ensuring a fair, transparent, and timely compensation process; and proposing habitat conservation measures, such as restoring suitable habitats for Asian elephants, establishing ecological corridors between nature reserves, and creating a nature reserve system based on the Asian Elephant National Park to enhance the habitat of Asian elephants. [ABSTRACT FROM AUTHOR]

An animal's capacity for innovation or solving novel problems likely has important implications for how quickly they can adapt to environmental change. Asian elephants, Elephas maximus , living in zoos have previously demonstrated a capacity to innovate, but problem solving has never been studied experimentally in a wild elephant population. We installed puzzle boxes with multiple possible solutions inside a protected area in western Thailand to determine individual variation in innovation, as well as other behavioural traits associated with elephants' problem solving, including persistence, exploratory diversity and neophilia. We recorded 77 elephants approaching the puzzle box, with 44 interacting with the box in their first exposure. Individuals varied widely in their success opening the doors of the puzzle box. Such success was influenced by persistence and exploratory diversity in both the first interaction as well as across multiple interactions. However, when considering each individual's overall innovation scores, which represented how many different doors elephants were able to open across all of their interactions with the puzzle box, only greater persistence and interaction number were associated with reaching a higher innovation score. We observed that elephants who interacted with the box multiple times learned to open a door of any type more quickly as their interactions increased, but we did not see evidence of learning to open specific door types over time. Overall, this study about how innovation and its associated behaviours vary in wild elephants not only informs our understanding of how a capacity for problem solving is expressed, but also how well elephants may be able to adapt to, overcome or avoid increasingly frequent interactions with humans within their natural habitat. • Innovation, or the ability to solve novel problems, has been studied in animals. • Innovation has rarely been studied in the wild and never before in wild elephants. • Wild elephants can innovate with variation between individuals in how well they do. • Persistence, exploration and number of interactions all impacted success. • Flexibility and innovation may help elephants adapt to environmental change. [ABSTRACT FROM AUTHOR]

Simple Summary: The infrequent northward migration of wild Asian elephants in Xishuangbanna, China, has attracted global attention due to its rarity. Elephant migration is a complex ecological process, and the factors driving this long-distance migration remain the subject of ongoing research. In this study, by comparing the diets and fecal metagenomes of breastfed elephant calves, captive elephants, and their wild counterparts, we revealed that wild elephants exhibit a preference for crops and grains with a low fiber content. Furthermore, their gastrointestinal tracts harbor distinct microbial communities, functional hydrolases, and metabolic pathways tailored for processing these foods. These findings suggest that wild elephants have adapted to favor a low-fiber diet. This dietary preference, shaped by their unique gut microbiota, provides a significant explanation for the uncommon long-distance migration observed in wild Asian elephants. The rare northward migration of wild Asian elephants in Xishuangbanna, China, has attracted global attention. Elephant migration is a complex ecological process, and the factors driving this long-distance migration remain elusive. In this study, fresh fecal samples were collected from both captive and wild Asian elephants, along with breastfed calves residing within the Wild Elephant Valley of Xishuangbanna. Our aim was to investigate the relationship between diet, gut microbiota, and migration patterns in Asian elephants through comprehensive metagenomic sequencing analyses. Among the breastfed Asian elephant group, Bacteroidales and Escherichia emerged as the dominant bacterial taxa, while the primary carbohydrate-active enzymes (CAZymes) enriched in this group were GH2, GH20, GH92, GH97, GH38, GH23, and GH43, aligning with their dietary source, namely breast milk. The bacterial taxa enriched in captive Asian elephants (CAEs) were mainly Butyrivibrio, Treponema, and Fibrobacter, and the enriched lignocellulose-degrading enzymes mainly included GH25, GH10, GH9, and cellulase (EC 3.2.1.4). These findings are consistent with the high-fiber diet of captive elephants. In contrast, the main bacterial taxa enriched in wild Asian elephants (WAEs) were Ruminococcus and Eubacterium, and the enriched CAZymes included GH109, GH20, GH33, GH28, GH106, and GH39. The abundance of lignocellulose-degrading bacteria and CAZyme content was low in WAEs, indicating challenges in processing high-fiber foods and explaining the low-fiber diet in this group. These findings suggest that wild elephant herds migrate in search of nutritionally suitable, low-fiber food sources. [ABSTRACT FROM AUTHOR]

This study aimed to predict the habitat suitability of Asian elephants in Madhesh Province, Nepal, by using maximum entropy (MaxEnt) modeling based on the occurrence data and environmental variables, including bioclimatic, topographic, vegetation‐related, and anthropogenic variables. The study was conducted under current and future climate scenarios for the year 2100. Among the districts of Madhesh Province, the largest suitable habitats for elephants are located in Rautahat district (30%) followed by Bara district (21%), with Dhanusha district having the smallest suitable area (1%). Elevation, slope, annual precipitation, precipitation of the driest quarter, and temperature seasonality were identified as the most important variables affecting habitat suitability. A total of 1037.3 km2 was identified as the current highly suitable habitat for elephants, primarily in grasslands and shrublands. The results of the study depict a slight increase in highly suitable areas under the emission scenarios shared socioeconomic pathways (SSP) 2–4.5 and SSP 5–8.5 but a severe decrease in suitable habitats and a drastic increase in unsuitable habitats. The research emphasizes the possible influence of human activities and land use on the living environment of elephants. The study, therefore, suggests that the authorities should prioritize future land use management to lessen the potential harm to the habitats of elephants and other endangered species in Nepal. It is also imperative to identify how elephants utilize their spatial habitat within their range to help park authorities devise efficient management strategies. [ABSTRACT FROM AUTHOR]

The Asian elephant is a conservation flagship species in tropical Asia, but credible population counts are only known for very few populations. This is because the species is secretive and resides in dense habitat across a vast portion of its range. Over the last two decades, the Lucknow and Jaipur districts of north India have seen an increase in human-elephant conflict. The little amount of somewhat protected habitat available to elephants in this human-dominated terrain does not appear to be sufficient to sustain them in the long term. Options for resolving the increasing dispute include elephant translocation, elephant confinement and culling. We conducted population genetic structure and conservation studies on Asian elephants in the Lucknow and Jaipur districts to ascertain the population’s size and genetic structure relative to the landscape. We obtained fresh dung samples from the field and genotyped those using eight microsatellite loci. In Lucknow and Jaipur districts, we identified a population size of 30 ± 8 (SE) and 62 ± 22 (SE) elephants, respectively. We illustrate how molecular data from noninvasive sampling and capture-recapture models can be used to influence management decisions. Numerous dangers to the population were discovered, including (i) the potential of inbreeding and associated loss of genetic variety, (ii) unauthorized elephant slaughter and (iii) a shortage of protected habitat. [ABSTRACT FROM AUTHOR]

Three cases of double infection with elephant endotheliotropic herpesvirus (EEHV) types 1A and 4 in captive Asian elephants are presented. The first calf was a 4-year-old female that showed initial signs of lethargy and depression. The second calf was a 6-year-old female that displayed signs of depression and diarrhea and died within 48 h of the start of supportive treatment. The third was a 2-year-old male that died suddenly while living with the herd. Necropsies were performed in the first and second elephants, while only a tongue sample was collected from the third calf. EEHV infection was confirmed via quantitative PCR (qPCR) and gene sequencing, revealing double subtypes of EEHV1A and -4 infections. This study describes the hematological and pathological characteristics within the host following double EEHV infection.

Abstract This study aimed to predict the habitat suitability of Asian elephants in Madhesh Province, Nepal, by using maximum entropy (MaxEnt) modeling based on the occurrence data and environmental variables, including bioclimatic, topographic, vegetation‐related, and anthropogenic variables. The study was conducted under current and future climate scenarios for the year 2100. Among the districts of Madhesh Province, the largest suitable habitats for elephants are located in Rautahat district (30%) followed by Bara district (21%), with Dhanusha district having the smallest suitable area (1%). Elevation, slope, annual precipitation, precipitation of the driest quarter, and temperature seasonality were identified as the most important variables affecting habitat suitability. A total of 1037.3 km2 was identified as the current highly suitable habitat for elephants, primarily in grasslands and shrublands. The results of the study depict a slight increase in highly suitable areas under the emission scenarios shared socioeconomic pathways (SSP) 2–4.5 and SSP 5–8.5 but a severe decrease in suitable habitats and a drastic increase in unsuitable habitats. The research emphasizes the possible influence of human activities and land use on the living environment of elephants. The study, therefore, suggests that the authorities should prioritize future land use management to lessen the potential harm to the habitats of elephants and other endangered species in Nepal. It is also imperative to identify how elephants utilize their spatial habitat within their range to help park authorities devise efficient management strategies.

Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species. The protein solution from pooled seminal plasma from 10 bulls (a total of 33 ejaculates) was digested into peptides and identified using LC-MS/MS. Out of 986 proteins, 597 were mapped and matched with 58 species in UniProt databases, including Elephas maximus. These mapped proteins were mostly involved in binding function, catalytic activity, cellular process, and metabolic process. Only 29 mapped proteins were recognized to be related in reproductive process, mainly associated in spermatogenesis and sperm capacitation. Additionally, several seminal plasma proteins related to fertility or semen quality in other mammals were also found in Asian elephant semen, such as keratin type I, aldose reductase, thrombospondon-1, fibronectin 1, platelet-activating factor acetyl hydrolase, mannosidase, and semenogelin-2. This discovery clearly reveals the beneficial protein profile in seminal plasma of the Asian elephant and serves as a crucial step in investigating infertility and poor semen quality in this valuable species.

Human-elephant conflict (HEC) is a severe conservation, socio-economic and environmental issue of forests and ecosystems in elephant inhabiting countries, including Sri Lanka. Due to the rapid growth of human and elephant populations, both struggles to share limited land resources. The major causes and contexts of HEC in Sri Lanka include land use change, habitat loss due to human population growth, crop raiding behavior, problem elephants, and changes in agriculture practices. Since 2019, 125 people and 370 elephants have killed annually on average due to the conflict. Also, Sri Lanka has recorded the highest annual elephant deaths and second-highest human deaths due to HEC. The human death rate has increased by approximately 42% over previous three decades. The Sri Lankan government provides compensation for death and disability of the human caused by elephants and for elephant-damaged houses or properties. The Sri Lankan elephant (Elephas maximus maximus) is an endangered subspecies. It's home range is restricted to 50–150 km2 and depends on the availability of food, water, and shelter of the habitat in which they live. Various management strategies have been developed by the government and villagers to prevent and mitigate HEC. Today, Sri Lankan elephants are protected under Sri Lankan law, with punishment by fines and jail terms. This article reviews the history, present status, and traditional conflict management of HEC in Sri Lanka. We suggest a satellite data fusion approach with GIS modeling to identify risk zones of HEC to develop further protective measures for humans and elephants. [ABSTRACT FROM AUTHOR]

Asian Elephants feed predominantly on grass. The comparative nutritional contribution of grasses and other elephant forage is not known. Therefore, the proximate nutrition of food plants selected by elephants, and the relationship of their diet composition to body condition and gender were examined in this study. Proximate analysis was conducted on 11 plant species recognised upon 66h of opportunistic focal animal sampling. Five species among them were grasses, including the invasive Megathyrsus maximus. The microhistological composition of freshly collected dung from 26 identified elephants was assessed against their body condition and gender. Associations, comparisons, and hypotheses were tested. Dicots were significantly high in dry matter and low in moisture, while monocots were high in moisture and low in dry matter (p <0.001). The average monocot: dicot ratio was 1: 0.73 in elephant diet. However, it was observed that the monocot composition in the male diet was significantly higher than dicots (p <0.001), while there was no significant difference in the female diet composition. Elephant body condition did not show any correlation with the abundance of monocot or dicot plant tissues. The preliminary study implies that dry matter nutrients in dicots and moisture in monocots influence diet selection of elephants. Their diet composition was associated with gender but did not correlate with body condition. M. maximus was not outstanding in nutrition from the selected plant species. [ABSTRACT FROM AUTHOR]

Simple Summary: Our study investigates a specific genetic loss and duplication in elephants by comparing genomic data from 12 different mammalian species. We created a synteny map and phylogenetic tree of the selected species, which suggested that elephant CYP1–4 genes have undergone independent duplication, particularly in the CYP2A, CYP2C, and CYP3A genes, a unique cluster found only in elephants. In contrast, CYP2E and CYP4A might have decayed to a state of genetic dysfunction (pseudogene). These results suggest the need for further investigations into elephant genomics to evaluate the species' metabolic mechanisms. Cytochrome P450 is an important enzyme that metabolizes a variety of chemicals, including exogenous substances, such as drugs and environmental chemicals, and endogenous substances, such as steroids, fatty acids, and cholesterol. Some CYPs show interspecific differences in terms of genetic variation. As little is known about the mechanisms of elephant metabolism, we carried out a comparative genomic and phylogenetic analysis of CYP in elephants. Our results suggest that elephant CYP genes have undergone independent duplication, particularly in the CYP2A, CYP2C, and CYP3A genes, a unique cluster specific to elephant species. However, while CYP2E and CYP4A were conserved in other Afrotheria taxa, their decay in elephants resulted in genetic dysfunction (pseudogene). These findings outline several remarkable characteristics of elephant CYP1–4 genes and provide new insights into elephant xenobiotic metabolism. Further functional investigations are necessary to characterize elephant CYP, including expression patterns and interactions with drugs and sensitivities to other chemicals. [ABSTRACT FROM AUTHOR]

The gut microbiome is closely related to host nutrition and health. However, the relationships between gut microorganisms and host lifestyle are not well characterized. In the absence of confounding geographic variation, we defined clear patterns of variation in the gut microbiomes of Asian elephants (AEs) in the Wild Elephant Valley, Xishuangbanna, China, along a lifestyle gradient (completely captive, semicaptive, semiwild, and completely wild). A phylogenetic analysis using the 16S rRNA gene sequences highlighted that the microbial diversity decreased as the degree of captivity increased. Furthermore, the results showed that the bacterial taxon WCHB1-41_c was substantially affected by lifestyle variations. qRT-PCR analysis revealed a paucity of genes related to butyrate production in the gut microbiome of AEs with a completely wild lifestyle, which may be due to the increased unfavorable environmental factors. Overall, these results demonstrate the distinct gut microbiome characteristics among AEs with a gradient of lifestyles and provide a basis for designing strategies to improve the well-being or conservation of this important animal species.

The Asian elephant is mostly confined to mountainous ranges and therefore risks population fragmentation if hard protected area (PA) boundaries near steep slopes prevent movement. We tested whether elephant gene flow is (i) controlled by slope and (ii) affected by the interplay between barriers and slope. We used 176 unique genotypes obtained non-invasively from fresh elephant dung to assess individual-by-individual genetic distance across the Western Ghats of India, a biodiversity hotspot. To assess landscape distance, 36 resistance models were produced by transforming a slope raster. Core areas and corridors were calculated from the raster that provided the best correlation between the genetic and distance matrices. The influence of the closure of PAs on gene flow was examined for one region, the Nilgiri Biosphere Reserve. The best resistance raster obtained by transforming the slope occupancy model was better than Euclidean distance for explaining genetic distance, indicating that slope partially controls gene flow. Fencing elephant PAs on hilly terrain reduces core areas and disrupts corridors. Consequently, hard PA boundaries abutting slopes can fragment elephant populations, but this can be ameliorated by protecting the adjacent flatter terrain.

Human–elephant conflict (HEC) contributes to the increasing death of Asian elephants due to road accidents, retaliatory killings and fatal infections from being trapped in snares. Understanding the diet of elephants throughout Peninsular Malaysia remains crucial to improve their habitat quality and reduce scenarios of HEC. DNA metabarcoding allows investigating the diet of animals without direct observation, especially in risky conflict areas. The aim of this study was to determine: i) the diet of wild Asian elephants from HEC areas in Peninsular Malaysia using DNA metabarcoding and ii) the influence of distinct environmental parameters at HEC locations on their feeding patterns. DNA was extracted from 39 faecal samples and pooled into 12 groups representing the different sample locations: Kuala Koh, Kenyir, Ulu Muda, Sira Batu, Kupang-Grik, Bumbun Tahan, Belum-Temengor, Grik, Kampung Pagi, Kampung Kuala Balah, Aring 10 and the National Elephant Conservation Centre, which served as a positive control for this study. DNA amplification and sequencing targeted the ribulose-bisphosphate carboxylase gene using the next-generation sequencing Illumina iSeq100 platform. Overall, we identified 35 orders, 88 families, 196 genera and 237 species of plants in the diet of the Asian elephants at HEC hotspots. Ficus (Moraceae), Curcuma (Zingiberaceae), Phoenix (Arecaceae), Maackia (Fabaceae), Garcinia (Clusiaceae) and Dichapetalum (Dichapetalaceae) were the highly abundant dietary plants. The plants successfully identified in this study could be used by the Department of Wildlife and National Parks (PERHILITAN) to create buffer zones by planting the recommended dietary plants around HEC locations and trails of elephants within Central Forest Spine (CFS) landscape.

Understanding the diet composition and preferences of large herbivores not only provides insights into their ecological role, but also helps to assess the viability of elephant populations and their habitats. This study was performed to determine the diet preferences of a small population of Asian elephants in Nangunhe National Nature Reserve in Yunnan, China, during the annual dry season. The next-generation sequencing of the rbcL gene from non-invasively collected fecal samples was conducted in addition to transect surveys and camera-trapping along known elephant trails. With the transect survey, we identified 31 plant species foraged by elephants. The next-generation sequencing analysis identified a total of 90 plant species from the elephant dung samples. Only nine species were detected at rates greater than 1% in all the samples. Poaceae (47.69%), Moraceae (21.25%), and Musaceae (11.24%) were detected to have the highest rates at the family level. We also examined whether differences existed between sexes, age groups, and individuals; however, significant differences were found only between individuals. This study provides useful insights into the foraging preferences of Asian elephants, which could help in further understanding the interactions between elephants and their habitat in the reserve and inform future management decisions in related areas. The detected core plant species with relatively high abundance could provide guidance for habitat restoration and cultivation of food bases. The local plantations where the elephants prefer to feed could be moved farther away, making them inaccessible to the elephants. [ABSTRACT FROM AUTHOR]

Simple Summary: Insufficient maternal milk is one of the important reasons for the low survival rate of young Asian elephants. Finding the optimal break milk supplementation for young Asian elephants is a matter of urgency. In our study, we investigated the microbiomes of young Asian elephants on different milk-containing diets (elephant milk only, elephant milk–plant mixed feed, and goat milk–plant mixed feed). Our results suggested that goat milk is not suitable for young elephants, and yak milk may be an ideal source of supplemental milk for Asian elephants. Evaluating the association between milk-containing diets and the microbiomes of young Asian elephants could assist establishing optimal breast milk supplementation to improve offspring survival rates. The microbiomes of young Asian elephants on different milk-containing diets (elephant milk only, elephant milk–plant mixed feed, and goat milk–plant mixed feed) were investigated using high-throughput sequencing of 16S rRNA genes and phylogenetic analysis. Microbial diversity was lower in the elephant milk-only diet group, with a high abundance of Proteobacteria compared to the mixed-feed diet groups. Firmicutes and Bacteroidetes were dominant in all groups. Spirochaetae, Lachnospiraceae, and Rikenellaceae were abundant in the elephant milk–plant mixed-feed diet group, and Prevotellaceae was abundant in the goat milk–plant mixed-feed diet group. Membrane transport and cell motility metabolic pathways were significantly enriched in the elephant milk–plant mixed-feed diet group, whereas amino acid metabolism and signal transduction pathways were significantly enriched in the goat milk–plant mixed-feed diet group. The intestinal microbial community composition and associated functions varied significantly between diets. The results suggest that goat milk is not suitable for young elephants. Furthermore, we provide new research methods and directions regarding milk source evaluation to improve elephant survival, wellbeing, and conservation. [ABSTRACT FROM AUTHOR]

Megaherbivores exert strong top-down influence on the ecosystems they inhabit, yet little is known about the foraging impacts of Asian elephants (Elephas maximus) on the structure of Southeast Asia’s rainforests. Our goal was to document Asian elephants’ dietary composition, selectivity, and foraging impacts in a Sundaic rainforest and test whether these differed between habitats. We conducted controlled direct observations of five wild-born captive elephants feeding on six plant types (bamboo, grass, monocot herbs, palms, lianas, and trees) of different age 2 in two habitats (mature vs. early successional forest) in Krau, Peninsular Malaysia. Palms, trees, and lianas formed the bulk of the elephants’ diet. In the mature forest, elephants showed a strong preference for monocots (preference ratio, PR = 5.1), particularly large palms (PR = 5.4), while trees were negatively selected (PR = 0.14). Conversely, in early successional habitats, large tree saplings were positively selected (PR = 1.6). Elephants uprooted (30%) and broke the main stem (30%) of the dicot trees, mainly large saplings, that they handled. Tree saplings broken by elephants had an average diameter of 1.7 ± 1.1 cm (up to 7 cm), with breaks happening at 1.1 ± 0.5 m of height. We estimated that, in a year, an elephant could damage (i.e., either uproot or break) around 39,000 tree saplings if it fed entirely in mature forest, and almost double the number (73,000) if it fed solely in early successional habitats. Assuming a density of 0.05–0.18 elephants/km2, elephant foraging could damage 0.2–0.6% of the tree sapling population per year. Slow growth rates of understory plants in mature forests could result in negative feedbacks, whereby elephants suppress palms, other monocots, and highly preferred tree species. Alternatively, elephants may initiate positive feedbacks by impeding succession along forest edges and in semi-open environments, thereby increasing the size of gaps and the availability of their preferred foodplants. Overall, our results show that Asian elephants act as ecological filters by suppressing the plants they prefer in Southeast Asia’s rainforests.

Endangered Asian elephants (Elephas maximus) find themselves at the center of debates involving politics, land use, human–wildlife conflict, and environmental justice. The intensity of such debates has led scholars to label conservation challenges as wicked problems with profound implications on local and global practice. In elephant range states such as Nepal, these debates are made more complex through human ‘ownership’ of endangered individuals for use in tourism, worship, or co-work. Human perspectives on the ethics of using animals for tourism are changing, even in areas heavily reliant on the tourism industry for survival. These debates become inflamed when non-residents take on adversarial positions despite an acceptance of the ‘ownership’ or ‘use’ of endangered individuals among local communities. Novel approaches are needed if there is any hope of establishing a common ground upon which to build relationships which may benefit community members, international interests, and endangered individuals.

Abstract Background Elephants are currently the largest mammals on earth. A comprehensive examination of the anatomy of this animal to diagnose various disorders is required. In addition, due to the heavy head of these animals, adaptations have been made in the anatomical structure of the neck that is worth studying. Objective This study aimed to investigate a standard morphologic and morphometric description of the elephant cervical spine. Another aim of this study was to compare the changes in the cervical skeleton of elephants with horses and cattle. Methods For this study, the cervical vertebrae of the Asian elephant, cattle and horse were examined. CT Images were obtained using Somatom Spirit II CT Machine. Statistical analysis was done by SPSS 24 software. Results Two dorsal tubercles and a groove between them were observed on the dorsal arch of the atlas vertebra of the Asian elephant. In elephant samples, the variation of vertebral body height, spinous process height, transverse process width, vertebral body length and vertebral foramen volume indices were statistically significant. The volume of the vertebral foramen in the elephant decreases in the second vertebra compared to the first vertebra, decreases in the third vertebra, decreases in the fourth, increases in the fifth, decreases in the sixth and increases in the seventh. Conclusions In this study, the structure of the cervical vertebrae of the Asian elephant was examined, and certain features were observed. One of the main features was the reduction of the length of the vertebrae, which leads to the decrease of the ratio of neck length to the size of the body. This condition can be due to the high weight of the head in the elephant. To maintain this weight, it is necessary to reduce the length of the neck and confer less mobility.

The Asian elephant (Elephas maximus) have a wide range of foraging needs and heterogeneous habitat preferences that bring them close to the forest edge. Currently, most of the range of wild elephants is located outside protected areas (PA). Nighttime light data (NTL) has been used as a component of human footprint due to its characteristics of characterizing the intensity of human activities and spatio-temporal continuity. We chose Xishuangbanna National Nature Reserve, the main distribution area of Asian elephants, as the study area and used NTL to monitor human activities, supplemented by land cover (LC) type data, to study the Asian elephant sanctuary and its surrounding areas, and found that: 1) NTL features inside and outside the PA are approaching, and the boundary effect of the sanctuary is weakening; 2) The NTL gathering area in the study area is mainly distributed in the south of the PA, 86.21% of the area’s nighttime lights have a significant increase trend, and the human activity range is evolving toward the southeast in the past decade; 3) the percentage of areas with a significant increasing trend of NTL under different LC types is greater than decreasing, nearly 50% of the forest showed an increasing trend of NTL. LC types with reduced forest have been converted to cropland and building in the last decade.

The Asian elephant (Elephas maximus Linnaeus) is a globally endangered species, an internationally protected species, and a first-class protected animal in China. However, future climate change and human activities exacerbate the instability of its habitat range, leading to a possible reduction in the range. By using multi-source remote sensing data and products, as well as climate change models, including ASTER GDEM v3, Landsat8 OLI image and ClimateAP, we examined the effects of ecological factors related to climate and natural and anthropogenic influences on the distribution of Asian elephants in Sipsongpanna. Multiyear elephant field tracking data were used with a MaxEnt species distribution model and the climate model. First, the distribution of Asian elephants in potentially suitable areas in Sipsongpanna was simulated under current climatic conditions without considering human activities. The predicted distribution was verified by existing Asian elephant migration trajectories. Subsequently, the distribution of potentially suitable areas for Asian elephants in Sipsongpanna was simulated under two climate change scenarios (RCP4.5, RCP8.5) in three periods (2025, 2055, and 2085). The changes in potentially suitable areas for Asian elephants in Sipsongpanna were analyzed under multiple climate change scenarios for the current (2017) and different future periods by considering the effects of human activities. The results show the following: (1) under anthropogenic interference (AI), the optimal MaxEnt model has a high prediction accuracy with the area under the curve (AUC) of 0.913. The feature combination (FC) includes linear, quadratic, and threshold features, and the regularization multiplier (RM) is 2.1. (2) Jackknife analyses of the non-anthropogenic interference (NAI) and anthropogenic interference (AI) scenarios indicate that topography (altitude (Alt)), temperature (mean warmest month temperature (MWMT)), and precipitation (mean annual precipitation (MAP)) are the top three factors influencing the distribution of Asian elephants. (3) The total area suitable for Asian elephants under current climate conditions and AI accounts for 46.35% of the total area. Areas of high suitability (occurrence probability >0.5) are located in Jinghong City in central Sipsongpanna and Mengla County in southeastern Sipsongpanna. Among them, the minimum habitat range and ecological corridors are mainly located in Mengman Town, Mohan Town, Mengla Town, Mengban Township, Dadugang Township, and Mengwang Township. (4) The change in potentially suitable areas for Asian elephants between current and future conditions is small under AI and large under undisturbed conditions. [ABSTRACT FROM AUTHOR]

Simple Summary: Supernumerary marker chromosomes, as they are known in the human population, are usually small chromosomes that differ morphologically and structurally from the standard ones and in many cases are formed by genetically inert heterochromatin. Similar features were observed for a supernumerary chromosome discovered in two Asian elephants, a mother and her male offspring. In this study, we present its detailed analysis using several molecular cytogenetic techniques including laser microdissection and fluorescence in situ hybridization that allowed identification of this marker chromosome. Based on our findings, we propose the most possible mechanism for the origin of the marker studied. We extended our investigation and showed that the distribution of nucleolar organizer regions on the chromosomes of Asian and savanna elephants may be related to the distribution of heterochromatin. Supernumerary chromosomes or, in other words, additional or extra chromosomes added to typical human or animal karyotypes, have recently gained the attention of scientists as model systems for the study of chromosome evolution, which may include the chromosome marker described here. We identified a small, supernumerary marker chromosome (sSMC) in two phenotypically normal Asian elephants (Elephas maximus): a female (2n = 57,XX,+mar) and her male offspring (2n = 57,XY,+mar). sSMCs are defined as structurally abnormal chromosomes that cannot be identified by conventional banding analysis since they are usually small and often lack distinct banding patterns. Although current molecular techniques can reveal their origin, the mechanism of their formation is not yet fully understood. We determined the origin of the marker using a suite of conventional and molecular cytogenetic approaches that included (a) G- and C-banding, (b) AgNOR staining, (c) preparation of a DNA clone using laser microdissection of the marker chromosome, (d) FISH with commercially available human painting and telomeric probes, and (e) FISH with centromeric DNA derived from the centromeric regions of a marker-free Asian elephant. Moreover, we present new information on the location and number of NORs in Asian and savanna elephants. We show that the metacentric marker was composed of heterochromatin with NORs at the terminal ends, originating most likely from the heterochromatic region of chromosome 27. In this context, we discuss the possible mechanism of marker formation. We also discuss the similarities between sSMCs and B chromosomes and whether the marker chromosome presented here could evolve into a B chromosome in the future. [ABSTRACT FROM AUTHOR]