Search

[SPA] El objetivo fue evaluar el contenido de compuestos fitoquímicos en brotes de brócoli mínimamente procesados iluminados durante 15 días a 5ºC con LEDs blancas, azules, verdes, rojas y rojas lejanas. Se utilizaron tratamientos de oscuridad y luz Fluorescente como controles. Los germinados bajo iluminación LED blanca, azul, verde, roja y roja lejana mejoraron la capturación de radicales libres tras 8 días a 5ºC en un 61, 58, 54, 68 y 36%, respectivamente, en comparación con la oscuridad. Todos los tratamientos LED mantuvieron dichos incrementos en el porcentaje de captación de radicales tras 15 días a 5ºC. Estos resultados están en concordancia con el aumento de los compuestos fenólicos, en los que los brotes iluminados con LEDs durante 15 días a 5ºC mostraron un aumento respecto a la luz fluorescente y la oscuridad. En conclusión, nuestros resultados sugieren que los germinados mínimamente procesados pueden beneficiarse de la iluminación LED durante su vida útil en términos de sus compuestos nutracéuticos, especialmente bajo iluminación roja. [ENG] The objective was to evaluate phytochemical content changes in minimally processed broccoli sprouts illuminated for 15 days at 5ºC with white, blue, green, red and far red LEDs. darkness and fluorescent light (FL) were used as controls. Sprouts under white, blue, green, red, and far red LED lighting improved the free radical scavenging after 8 days at 5ºC by 61, 58, 54, 68, and 36%, respectively, compared to darkness. All LED treatments maintained these increases in the percentage of radical scavenging after 15 days at 5ºC. Such results agree with the increase in phenolic compounds, where sprouts illuminated with LEDs for 15 days at 5ºC showed an increase compared to FL and darkness. In conclusion, our results suggest that minimally processed sprouts can benefit from LED illumination during their shelf life in terms of their nutraceutical compounds, especially under red illumination. Se agradece la financiación a la Comunidad Autónoma de la Región de Murcia a través del Programa Regional de Fomento de la Investigación Científica y Técnica de la Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia (Proyecto Ref 20849/PI/18) y al Ministerio de Economía y Competitividad la concesión de un contrato predoctoral a Noelia Castillejo (FPU/04763).

Figs are an important constituent of the Mediterranean diet, widely consumed fresh. Due to their very short shelf life, postharvest techniques to preserve quality and safety should be implemented. The current work aimed to study the combined effect of a postharvest UV–C treatment (5 or 10 kJ m−2) followed by packaging under passive modified atmosphere (MAP) on physicochemical and bioactive quality of fresh figs (cv. ‘Colar’) stored up to 12 d at 0 ºC (90–95% RH). As controls, UV–C untreated and air-stored (AIR) samples were used. The flesh firmness, skin colour, soluble solids content (SSC), titratable acidity (TA), pH, total phenolic content and total antioxidant activity were monitored. The steady-state modified atmosphere reached since day 8 was 17 kPa CO2 and 4 kPa O2. UV-C treated samples did not show visual decay incidence, while mycelial growth was observed in untreated figs on day 12. Figs treated with 10 kJ m-2 UV–C and stored under MAP conditions showed the highest flesh firmness with values of 0.6 N, which is 50% higher than in AIR. Skin darkening observed among samples was higher in 10 kJ m-2 with Chroma decreases of 2.6 units on day 12 under MAP, although panellists preferred this treatment according to its visual appearance. Higher SSC and TA differences were found in UVC-treated samples during storage. Moreover, 10 kJ m-2 UV–C and MAP induced the highest total phenolic content accumulation, with 260% and 540% higher values from day 4 to day 12 for skin and flesh, respectively. In conclusion, a 10 kJ m−2 UV–C treatment after harvesting followed by packaging under MAP conditions may be considered a good tool to preserve the physicochemical and bioactive quality of fresh figs during 12 d at 0 ºC. The authors express their gratitude to CAPES (Higher Education Personnel Improvement Coordination) for a research grant (8578/11–7) made to Manoel Souza. The authors are grateful to the Tunisian Ministry of Higher Education and Scientific Research for a predoctoral grant to Monia Jemni. The authors express want also to thank Pablo Melgarejo (Universidad Miguel Hernández) for providing the plant material used in this study.

[SPA] Los germinados de Brassicas poseen un elevado contenido de compuestos nutricionales y bioactivos tales como polifenoles, flavonoides, antioxidantes y glucosinolatos. La aplicación de luz UV-B a frutas y hortalizas estimula la biosíntesis de fitoquímicos. Por ello el objetivo fue determinar si diferentes estreses con UV-B durante la germinación de semillas de col roja perjudicaban su desarrollo. La luz UV-B se aplicó en 4 dosis iguales en los días 3, 5, 7 y 10 de germinación a 20 °C que sumaron un total de 0, 5, 10 y 15 kJ m-2. Se determinó la longitud del hipocótilo de los germinados y su velocidad de crecimiento. Los germinados bajo UV-B tuvieron una mayor longitud del hipocótilo y bajo 10 kJ m-2 crecieron más rápidos. Así pues, dosis controladas y aplicadas de forma discontinua pueden favorecer el crecimiento de los germinados. [ENG] Brassica sprouts are a source rich in nutritional and bioactive compounds such as polyphenols, flavonoids, antioxidants and glucosinolates. In addition, the application of UV-B to fruit and vegetables increases phytochemical biosynthesis. The aim of this work was to determine whether different UV-B stresses during red cabbage seeds germination influenced the development. The UV-B light was applied in 4 equal doses on days 3, 5, 7 and 10 of germination at 20 °C, with a total dose of 0, 5, 10 and 15 kJ m-2. The length of the hypocotyl of the sprouts was determined, as well as their growth rate. Sprouts under UV-B had a longer hypocotyl length and 10 kJ m-2 induced a faster growth. Our results show that controlled and discontinuously applied doses of UV-B can promote sprout growth. Se agradece la financiación a la Comunidad Autónoma de la Región de Murcia a través del Programa Regional de Fomento de la Investigación Científica y Técnica de la Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia (Proyecto Ref 20849/PI/18). Se agradece al Ministerio de Economía y Competitividad la concesión de un contrato predoctoral a Noelia Castillejo (FPU/04763).

[SPA] En los últimos años se ha experimentado un notable interés en los germinados de semillas por ser productos muy nutritivos y saludables, siendo incluso más ricos en compuestos beneficiosos para la salud que la planta desarrollada como es el caso de las crucíferas. El presente trabajo estudia el efecto del metil jasmonato (MJ), como elicitor químico, durante la germinación de brotes de mostaza en condiciones de ciclo de luz normal y oscuridad. Los germinados CTRL presentaron la mayor longitud de hipocótilo tanto bajo condiciones de luz (6,31 cm) como de oscuridad (9,51 cm), mientras que las diferentes concentraciones de MJ supusieron una disminución, siendo mayores bajo la condición de luz. El porcentaje de germinación en el CTRL fue del 97,3% tanto en condiciones de luz y oscuridad mientras que con MJ disminuyeron hasta valores de 93,5-95% en condición de oscuridad. Mientras, que en condiciones de luz se mantuvo constante. Según estos datos preliminares, el MJ no influyó significativamente en la productividad, siendo por tanto muy interesante su estudio como estrés abiótico para potenciar los compuestos bioactivos en futuros ensayos. [ENG] In recent years there has been a notable interest in sprouts for being very nutritious and healthy products. The sprouts are richer in bioactive compounds than the developed plant. The propose of this work was to study the effect of methyl jasmonate (MJ), as a chemical elicitor, during the germination of mustard seeds under the normal light and dark conditions. The CTRL sprouts had the longest hypocotyl length under both light (6.31) and dark (9.51 cm) conditions, while the different concentrations of MJ resulted in a decrease of the hypocotyl length, being greater under the light condition. The percentage of germination in the CTRL was 97.3% in both light and dark conditions while with MJ they tended to decrease to values of 93.5-95% in dark condition. However, the germination percentage remained constant in light conditions. According to these preliminary data, MJ did not significantly influence in the productivity, being therefore very interesting its study as abiotic stress to enhance bioactive compounds in future trials. Se agradece la financiación a la Comunidad Autónoma de la Región de Murcia a través del Programa Regional de Fomento de la Investigación Científica y Técnica de la Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia (Proyecto Ref 20849/PI/18). Se agradece igualmente al Ministerio de Economía y Competitividad la concesión de un contrato predoctoral a Noelia Castillejo (FPU/04763).

[SPA] Kale es una verdura con un alto contenido en compuestos beneficiosos para la salud, pero su consumo como bebida está muy limitado por su sabor amargo. Se estudió el efecto de diferentes extractos de stevia (S10, S25 y S50) sobre el contenido de compuestos fenólicos totales (TPC) y capacidad antioxidante total (TAC) mediante DPPH y FRAP de esferas de zumo de kale durante 7 días a 5 °C. Las esferas se produjeron con una técnica de doble esferificación que permitió obtener esferas de hidrogel con alta resistencia mecánica. El TPC de las muestras S25 mostró el valor más bajo en el día 0, con diferencias en relación a las otras muestras. La mayor TAC se observó en las muestras evaluadas por FRAP. Tras 3 días de almacenamiento, se observó una disminución del TAC (por DPPH y FRAP). Estos resultados determinaron que se pueden producir esferas de zumo de kale con diferentes extractos de stevia y con altos niveles de antioxidantes. [ENG] Kale is a vegetable with high contents of health-promoting compounds, but its consumption as a beverage is highly limited by its bitter flavour. So, the effects of different stevia extracts (S10, S25 and S50) on the total phenolic contents (TPC) and total antioxidant capacity (TAC) by DPPH and FRAP of kale juice spheres was studied during 7 days at 5 °C. Spheres were produced with a double spherification technique which allowed to obtain hydrogel spheres with high mechanical resistance. The TPC of S25 samples showed the lowest value at day 0, with differences between other samples. The higher antioxidant capacity was observed in samples evaluated by FRAP, without differences between samples. After 3 days of storage, a decrease in TAC evaluated in both assays studied (DPPH and FRAP) was observed. These results determined that kale juice spheres with different stevia extracts and with high antioxidants levels can be produced. Gratitude is expressed to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil for a grant support to Tâmmila Venzke Klug. Authors are grateful to Sakata Seeds Ibérica S.L.U. for financial support and to Sacoje SCL for providing the plant material.

[SPA] El consumo de hortalizas puede incrementarse con la incorporación de nuevos productos como un pesto a base de kale, que es rico en compuestos bioactivos. Además, la revalorización de subproductos agrícolas permite aprovechar los generados diariamente. En este trabajo se presentan los resultados de dos salsas tipo ‘pesto’ de kale (una con hojas de brócoli y otra sin ellas -CTRL-) durante 21 a 5ºC. No hubo diferencias en el contenido de polifenoles/capacidad antioxidante en la Salsa+Subproducto con respecto a la salsa CTRL, salvo en el día 21 donde se observó una disminución del 30,66/55,22%. El análisis sensorial de ambas salsas obtuvo valores superiores a 3 (límite de comercialización) durante la conservación, destacando la Salsa+Subproducto. Por tanto, la revalorización del subproducto de brócoli para un pesto a base de kale no indujo diferencias en el contenido de compuestos bioactivos e incluso mejoró la calidad sensorial. [ENG] Vegetable consumption can be increased by the development of new products such as a pesto sauce including kale, because this vegetable has a high content of bioactive compounds. In addition, revalorization of broccoli by-products helps to manage the daily generated high agricultural wastes. This work presents data related to quality and bioactive contents changes of two kale-based sauces (including or not -CTRL- broccoli leaves) throughout 21 days at 5ºC. During storage no differences on total phenolics/ antioxidant capacity between sauces without or with broccoli by-product were found, except an 30.66/55.22% increase on day 21 on Sauce+Byproduct regarding CTRL. Sensory analyses of both pesto sauces showed scores higher than the limit of usability throughout the storage. Sauce+Byproduct pesto sauce was preferred by panelists. Therefore, the revalorization of broccoli by-product for a pesto sauce did not induce differences in the bioactive compounds content and even improved its sensory quality. Se agradece al Ministerio de Economía y Competitividad la concesión de un contrato predoctoral (FPU) a Noelia Castillejo y a SAKATA SEED IBÉRICA S.L.U. la financiación y aporte de material vegetal.

[SPA] Las habas (Vicia faba) tienen un alto contenido proteico y energético y son una fuente de minerales, fibra, vitaminas y antioxidantes. Además, el estilo de vida actual, con poco tiempo para preparar comidas equilibradas, y el creciente interés por una alimentación sana, ha llevado a una demanda de productos naturales, frescos y listos para consumir. Para mantener el efecto beneficioso de estos productos es necesario optimizar su procesado y los métodos de conservación. Por ello, la producción de semillas de habas inmaduras mínimamente procesadas sería una alternativa para promover su consumo. En este estudio se analizaron los efectos de la desinfección con NaOCl (150 ppm) o alternativamente con ácido ascórbico (AA) (0,05 mol L-1) o con zumo de limón (ZL) (30 ml 100 mL-1), y la conservación a 1 y 4°C, sobre la calidad sensorial (calidad general, apariencia visual, sabor y pardeamiento) y el contenido en fenoles totales y capacidad antioxidante. El almacenamiento a 1°C prolongó la vida útil de las habas mínimamente procesadas bajo condiciones de MAP respecto al almacenamiento a 4°C. Los diferentes desinfectantes no afectaron al contenido en fenoles totales y a la capacidad antioxidante. [ENG] Faba beans (Vicia faba) are high in proteins and rich in energy with important health promoting minerals, dietary fiber, vitamins and antioxidants. In addition, today's lifestyle, with little time to prepare balanced meals and the increased interest in healthy food, has driven demand for natural, fresh, ready-to-eat vegetable products. To maintain the beneficial properties of these products is necessary to optimize the processing and preservation methods. According to that, the production of minimally processed immature legume seeds would be an alternative to promote the consumption of vegetables. The effects of sanitation with NaOCl (150 ppm) or alternatively with ascorbic acid (AA) (0.05 mol L-1) or lemon juice (LJ) (30 ml 100 mL-1) and storage at 1°C or 4°C on sensory attributes (overall quality, taste, appearance and browning), total phenolic content and antioxidant capacity were analyzed. Storage at 1°C extends the shelf life of minimally processed immature seeds stored under MAP conditions respect to storage at 4°C. Different disinfectant not affect to total phenolic content and antioxidant capacity. Al Proyecto EUROLEGUME financiado por fondos de la Unión Europea dentro del 7º Programa Marco de Investigación, Desarrollo Tecnológico y Diseminación, acuerdo Nª 613781, por la financiación recibida.

[SPA] Los batidos vegetales son una fuente rica de vitaminas, folatos, glucosinolatos, polifenoles, antioxidantes y minerales, entre otros, que complementan la dieta si se eligen bien sus ingredientes. Las algas son ricas en polifenoles y antioxidantes con actividad biológica. En este trabajo se presentan los resultados de polifenoles y antioxidantes de 3 batidos verdes a base de uva (56,5%), pepino (25,8%) y brócoli Bimi (15,5%), dos de ellos con alga (2,2%) y uno Control. Tras 24 días de conservación a 5ºC, el batido con alga Dulse tuvo un mayor contenido en fenoles (72,82 mg equivalente de ácido clorogénico 100 g-1 pf) que el Control (64.53 mg equivalente de ácido clorogénico 100 g-1 pf), mientras que el batido con Musgo de Irlanda fue similar al Control. No se encontraron diferencias significativas en la capacidad antioxidante entre el batido Control y con Dulse (30,87 y 29,02 mg trolox equivalent 100 g-1 pf) mientras que en el batido con Musgo de Irlanda el contenido fue menor (11,87 mg trolox equivalent 100 g-1 fw). Por tanto, la adición del alga Dulse al batido indujo un aumento de compuestos bioactivos muy interesante como complemento para la dieta. [ENG] Many vegetable smoothies are commonly a rich source of vitamins, folates, glucosinolates polyphenols, antioxidants, and minerals, among others, which can complement the diet if ingredients are properly chosen. Algae are rich in polyphenols and antioxidants that have biological activity. In this work the levels of polyphenols and antioxidants of 3 green smoothies based on table grape (56.5%), cucumber (25.8%) and Bimi broccoli (15.5%), two of them with algae (2.2%) and one used as Control are reported. After 24 days of storage at 5ºC, it was found that the smoothie with Dulse algae had a higher phenols content (72.82 mg chlorogenic acid equivalent 100 g-1 fw) than Control (64.53 mg chlorogenic acid equivalent 100 g-1 fw), while in the smoothie with Irish Moss algae it was similar than in Control. No differences in the antioxidant capacity between Control and Dulse smoothies (about 30 mg trolox equivalent 100 g-1 fw) were found while Irish Moss smoothie showed a lower content (11.87 mg trolox equivalent 100 g-1 fw). Therefore, the addition of Dulse algae to a green smoothie lead to an increase in bioactive compounds, which is very interesting as a diet complement. Se agradece la financiación del Ministerio de Economía y Competitividad (AGL2013-48830-C2-1-R), de SAKATA SEED IBÉRICA S.L.U. y a los fondos FEDER de la UE.

[SPA] Los purés de legumbres desarrollados bajo tecnologías eco-innovadoras son un mercado interesante, ya que los consumidores están demandando productos saludables listos para consumo debido al estilo de vida. El presente estudio evalúa la capacidad antioxidante total (TAC) por dos métodos diferentes (DPPH y FRAP) de un puré de guisantes durante 24 días a 5 °C. Los guisantes se trataron con vapor (5 min, 80 °C) o en un horno microondas (MW) semindustrial de flujo continuo (9 kW, 40 s), antes de ser triturados en un procesador (3 min, 3000 rpm) para obtener un puré consistente. Posteriormente se envasaron al vacío. El Control (CTRL) consistió en guisantes frescos triturados en el procesador (3 min, 3000 rpm) a temperatura ambiente y envasado al vacío. El TAC del puré de guisantes evaluado por FRAP y DPPH aumentó especialmente después del tratamiento con MW. Esta tendencia se observó hasta al final del almacenamiento, con diferencias en los tratamientos con vapor y CTRL en relación al ensayo de DPPH. La menor capacidad antioxidante se observó en muestras CTRL de puré. Estos resultados apuntan el calentamiento MW en las semillas de guisantes, como una técnica adecuada para mejorar la calidad del puré de guisantes. [ENG] Legume puree development under eco-innovative technologies is an interesting market. Consumers are demanding appetizing, healthy ready-to-eat products due to a desire for a healthier lifestyle. The present study evaluates the total antioxidant capacity (TAC) of two different assays (DPPH and FRAP) of a pea puree throughout 24 days of storage at 5 °C. Peas were treated with steam (5 min, 80°C) or continuous microwave (MW) flow (9 kW, 40s) before being blended in a food processor (3 min, 3000 rpm) to obtain a consistent puree and then vacuum packed. Control (CTRL) samples consisted on fresh blended peas in a food processor (3 min, 3000 rpm) at room temperature and vacuum packaged. The TAC of the pea puree, evaluated in both FRAP and DPPH assays studied increased, especially after MW treatment. This trend was observed at the end of the storage, reporting differences with to steam and CTRL treatments in relation to DPPH assay. The lowest TAC was observed in CTRL puree samples. These results determined that MW heating of pea seeds is an appropriate technique to improve the quality of a pea puree. Authors are grateful to EUROLEGUME Project Nº 613,781 funded by European Union under the 7th Framework Programme for Research, Technological Development and Dissemination. The authors also express gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil for a grant support to Tâmmila Venzke Klug.

Kale is a vegetable with high contents of health–promoting compounds although its consumption as a beverage is highly limited due to its bitter flavour. Nonetheless, the bitter flavour of Brassicas may be masked by sweetening. Then, the effects of different stevia extracts (CTRL, S0.5 (g L–1), S1.25 and S2.5), added to an innovative kale beverage presentation, were studied on the kale juice spheres quality during 7 days at 5ºC. Kale juice spheres were produced with a double spherification technique, which allowed to obtain hydrogel spheres with high mechanical resistance. The authors express their gratitude to CNPq (Council for Scientific and Technological Development, Brazil) for a doctoral grant (232758/2014−0) made to Tâmmila Venzke Klug. The authorsare grateful to S.G. Hasa for her skillful technical assistance. The authors are also grateful to Sakata Seeds Ibérica SLU and to Sacoje SCL for providing the plant material.

[SPA] Las habas (Vicia faba) son una de las leguminosas más comunes, fuente de proteínas, carbohidratos, antioxidantes, fibra, vitaminas y minerales. Por ello, la producción de semillas de habas inmaduras mínimamente procesadas sería una alternativa para promover su consumo. Además, este producto podría cocinarse en microondas si se envasa en un envase apropiado. En este estudio se analizaron los efectos de la desinfección con NaOCl (150 ppm, pH 6,5) o alternativamente con NaOCl + un recubrimiento comestible de ésteres de azúcar (Naturcover® P, NAT) o con luz UV-C (3 kJ m-2, UV) sobre la calidad sensorial (aspecto general, aroma, apariencia, textura, pardeamiento, deshidratación y pérdida de brillo) y la evolución de taninos, un factor anti-nutricional, en habas frescas y microondadas (1 min, 700 W). Los tratamientos NAT y UV permitieron que las semillas mínimamente procesadas y envasadas en atmósfera modificada pasiva llegaran a una vida útil de 10 días a 4°C, la que fue de 7 días para las tratadas con NaOCl. UV mostró un efecto positivo en la reducción de los taninos. La cocción con microondas redujo los taninos y mantuvo la calidad de las semillas obteniéndose un producto de alta calidad. [ENG] Faba seeds (Vicia faba L.) are one of the most common legumes, rich in proteins, carbohydrates, antioxidants, fiber, vitamins and minerals. Therefore, the production of minimally processed immature faba seeds would be an alternative to promote its consumption. In addition, that product can be microwaved if packaged in an appropriate package. The effects of sanitation with NaOCl (150 ppm, pH 6.5) or alternatively NaOCl + a sucroesther edible coating (Naturcover® P, NAT) or UV-C light (3 kJ m-2, UV) on sensory attributes (overall quality, taste, aroma, visual appearance, texture, browning, dehydration and brightness) and evolution of tannins, an antinutritional factor, on fresh and microwaved faba beans were analyzed. NAT or UV treatments allowed a shelf life of 10 days at 4°C while 7 days were possible for NaOCl treated fresh seeds stored in passive modified atmosphere. UV was effective to decrease tannins concentration. On the other hand, microwaving also decreased the tannins amount for all the treatments and allowed to obtain a product of high quality. A la financiación por parte del Proyecto EUROLEGUME, Fondos de la Unión Europea, 7º Programa Marco de Investigación, Desarrollo Tecnológico y Diseminación, Acuerdo Nº 613781.

Background: The heating of a green smoothie during an innovative semi-continuous microwave treatment (MW; 9 kW for 15 s) was modelled. Thermal and dielectric properties of the samples were previously determined. Furthermore, the heating effect on the main chemopreventive compounds of the smoothie and during its subsequent storage up to 30 days at 5 or 15 °C were studied. Such results were compared to conventional pasteurization (CP; 90 °C for 45 s) while unheated fresh blended samples were used as control (CTRL). Results: A procedure was developed to predict the temperature distribution in samples inside the MW oven with the help of numerical tools. MW-treated samples showed the highest sulforaphane formation after 20 days, regardless of the storage temperature, while its content was 2-fold reduced in CP samples. Storage of the smoothie at 5 °C is crucial for maximizing the levels of the bioactive compound S-methyl cysteine sulphoxide. Conclusion: The proposed MW treatment can be used by the food industry to obtain an excellent homogeneous heating of a green smoothie product retaining high levels of bioactive compounds during subsequent retail/domestic storage up to one month at 5 °C. The authors are grateful to Spanish Ministry of Economy and Competitiveness (MINECO) Project AGL2013-48830-C2-1-R and FEDER for financial support. We are also grateful to M. Otón for his skillful technical assistance

[SPA] Las zanahorias son la sexta verdura más consumida. Sin embargo, el contenido de los compuestos bioactivos fenólicos de las zanahorias no es muy alto. Los fenoles/antioxidantes de zanahorias pueden ser enormemente incrementados mediante diferentes estreses abióticos postcosecha. De esta forma, se preparó un smoothie funcional de zanahorias con altos niveles de fenoles/ antioxidantes y se estudiaron los cambios en su calidad bioactiva/fisicoquímica tras 25 días a 5 °C. Los compuestos fenólicos en los smoothies de zanahorias preincubadas en hiperoxia se incrementaron hasta un 1000% tras la conservación, aunque otros compuestos antioxidantes se redujeron en un 44%. [ENG] Carrots occupy the sixth place among the list of most consumed vegetables, although the content of the bioactive compounds phenolic acids is almost the lowest one. The phenolic/antioxidant levels of carrots may be greatly enhanced with a pre-enrichment incubation with different postharvest abiotic stresses. Accordingly, a functional carrot smoothie with phenolic /antioxidant-enriched levels was produced and its bioactive/physicochemical quality was monitored after 25 days at 5 °C. The total phenolics content from hyperoxia-preincubated carrot shreds was incremented up to 1000% after storage although other antioxidants compounds were reduced by 44%. Los autores agradecen al Ministerio de Economía y Competitividad Proyecto MINECO AGL2013-48830-C2-1-R la financiación recibida para este trabajo.

[SPA] Se ha estudiado el efecto de un tratamiento de pasteurización (90 ± 2 °C, 35 s) en un batido anaranjado de hortalizas. Este tratamiento térmico se aplicó con un microondas en continuo (MWP), utilizando diferentes dosis (baja potencia/tiempo largo y alta potencia/tiempo corto), comparándolo con un tratamiento térmico convencional (CP). Se logró una mejor retención del color (estabilidad del °hue), un incremento de la viscosidad y una mayor reducción de la actividad enzimática de peroxidasa, pectin metil esterasa y poligalacturonasa, en las muestras tratadas con MW, en particular, cuando se utilizaban los tratamientos de altas potencias y tiempos cortos. [ENG] The effect of a pasteurization treatment at 90 ± 2 °C for 35 s, provided by continuous microwave (MWP) under different doses (low power/long time and high power/short time) or conventional pasteurization (CP) on the quality of orange smoothies was studied. A better color retention of the MWP-treated smoothie using high power/short time than in conventionally-processed sample was evidenced by the stability of the hue angle. The continuous MW heating increased the viscosity of the smoothie more than the CP in comparison with non-treated samples. Lower residual enzyme activities from peroxidase (POD), pectin methylesterase (PME) and polygalacturonase (PG) were obtained under MW heating, specifically when highest power/shortest time was used. Thanks are due to the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (MINECO-FEDER, AGL2013-48830-C2-1-R) for financial support.

[SPA] Los consumidores demandan asiduamente nuevos productos saludables, seguros y listos para su uso. Por ello, los purés a base de hortalizas surgen como productos interesantes en la industria alimentaria. La calidad sensorial y nutricional de estos productos debe ser máxima para tener una buena aceptación por el consumidor, preservando en todo momento su seguridad alimentaria. Por ello, es necesario el procesado mediante técnicas menos agresivas, y ecoinnovadoras, que preserven la calidad de la materia prima durante una adecuada vida útil, frente a un tratamiento térmico convencional. El objetivo será estudiar el efecto de la alta presión hidrostática, una pasteurización por microondas de flujo continuo y otros métodos alternativos de calentamiento sobre el efecto en la calidad sensorial, nutritiva y microbiológica en diversos purés a base de hortalizas durante su vida comercial. [ENG] Frequently consumers demand new healthy, safe and ready to use products. Therefore, based purees vegetables arise as interesting products in the food industry. Sensory and nutritional quality of these products must be paramount to have good consumer acceptance, preserving food security. Therefore, processing by less aggressive techniques, and eco-innovative, which preserve the quality of the raw material for an adequate shelf life is necessary in relation a conventional heat treatment. The aim will be to study the effect of high hydrostatic pressure, flow microwave system pasteurization and other alternative heating methods on the effect on sensory, nutritional and microbiological quality in puree-based vegetables beverages during shelf life. Se agradece la financiación recibida por parte del Ministerio de Economía y Competitividad (MINECO), Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad AGL2013-48830-C2-1-R, a SAKATA SEEDS IBÉRICA, al Proyecto EUROLEGUME (EU FP7 Research Project No. 613781) de UE dentro del 7º Programa Marco de Investigación, Desarrollo Tecnológico y Diseminación y a los fondos FEDER. Se agradece especialmente la ayuda de la CNPq de Brasil por la beca de investigación concedida a Tâmmila V. Klug.

[SPA] Se ensayaron diversos agentes antipardeantes (ácido ascórbico, ácido peroxiacético y ácido etilen diamino tetra acético-EDTA) para prevenir el pardeamiento en semillas de haba mínimamente procesadas en fresco. En primer lugar, se aplicaron los diferentes antipardeantes descritos en una línea de procesado industrial, lavando 2 min a 5 °C y enjuagando 1 min, si era necesario, en combinación con 2 films a base de polipropileno (PP) para generar una atmósfera modificada (AM) durante 10 días a 4±1 °C. Dichos films fueron PP biorientado -BOPP- y PPlus®. Tras los resultados obtenidos, en un segundo ensayo, realizado en la UPCT, se usó un único film (BOPP) durante 9 días a 4±1 °C. En todos los casos la temperatura del agua de lavado fueron 5 °C. En el ensayo a escala industrial, la AM no fue adecuada, perjudicando la calidad de las habas. Sin embargo, en condiciones controladas en la UPCT existió una estabilización de la atmósfera y se mantuvo la calidad de las habas. Se necesitará profundizar en el control de parámetros a nivel industrial. [ENG] Several antibrowning agents (ascorbic acid, peroxyacetic acid and ethylene diamine tetra acetic-EDTA) were tested to prevent browning in the washing step of minimally processed Vicia faba seeds. In a first test, antibrowning agents were applied in an industrial process by washing for 2 min and rinsed for 1 min, if necessary, in combination with 2 polypropylene (PP) films to generate a modified atmosphere (MA) during 10 days at 4±1 °C. These films were bi-axially orientated PP -BOPP- and PPlus®. Considering the results of this test, a second test was made in the UPCT facilities. An OPP film was used for packaging after washing with the antibrowning agents during a shelf life of 9 days at 4±1 °C. The water temperature in all cases was 5 °C. At industrial scale, the MA generated was inadequate, which induced quality deterioration of the seeds. However under controlled conditions in the UPCT facilities, the atmosphere stabilization was enough to preserve the quality. It will be needed to control the parameters at industrial scale. Los autores agradecen a Frutas Esparza S.A. y al proyecto europeo EUROLEGUME FP7-BBBE.2013.1.2-02 la financiación recibida

[SPA] El ritmo de vida y los hábitos alimentarios actuales son las causas por las que los consumidores demandan alimentos sanos, nutritivos, seguros, respetuosos con el medio ambiente y listos para su consumo. Por ello los batidos de hortalizas frescas ricos en compuestos bioactivos pueden ser atractivos para el consumidor por satisfacer dichas necesidades, como un complemento en la dieta, rápido y fácil de consumir. El mercado de los batidos de hortalizas frescas es incipiente y no hay mucha información científica, la cual es necesaria para conocer los parámetros óptimos para una correcta conservación y una vida útil comercial lo más larga posible preservando su calidad y seguridad alimentaria. Por ello es imprescindible la investigación, desarrollo e innovación que estudie nuevas técnicas que mantengan o incrementen la calidad y seguridad, así como prolongar su comercialización. [ENG] Actual world life and current eating habits are the reasons why consumers demand healthy, nutritious, safe and eco-friendly ready to eat food. Therefore, fresh vegetables smoothies, rich in bioactive compounds can be attractive to the consumer to meet those needs, as a dietary supplement, quick and easy to consume. The market for fresh vegetables smoothies is still incipient and scientific information is very scarce, which is necessary to know the optimal parameters for proper conservation and commercial life as long as possible while preserving its quality and food safety. It is therefore essential to research, develop and innovation in order to explore new eco-friendly minimal processing techniques for keeping or increasing quality and safety as well as extend their marketing period. Se agradece la financiación recibida del Ministerio de Economía y Competitividad, Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad AGL2013-48830-C2-1-R, de SAKATA SEED IBÉRICA S.L.U. y a los fondos FEDER de la UE.

[SPA] Los guisantes (Pisum sativum L.) son una fuente importante de proteínas, carbohidratos, vitaminas y minerales. Debido a su alta tasa respiratoria, se deterioran rápidamente. Existe muy poca información sobre las condiciones óptimas de almacenamiento de semillas de guisantes inmaduras, pese a su conveniencia para utilizarse como producto mínimamente procesado. El objetivo de este trabajo fue analizar la calidad microbiológica y los cambios en la vitamina C de semillas inmaduras almacenadas a 1 y 4 °C en atmósferas modificadas (PPO 25 μm). Las semillas se lavaron con hipoclorito sódico (HS) (100 ppm, pH 6,5) o con clorito sódico acidificado (CSA) (300 ppm, pH 1,8). Después de 14 días, la atmósfera alcanzada fue de 8 kPa CO2/12 kPa O2 y 11 kPa CO2/10 kPa O2 a 1 y 4 °C, respectivamente. El crecimiento de mesófilos se redujo con la conservación a 1 °C, independientemente del desinfectante utilizado, mientras que las enterobacterias decrecieron en todos los tratamientos. La vitamina C disminuyó desde ≅ 700 mg kg-1 pf hasta 250 o 450 mg kg-1 pf, para HS y CSA respectivamente. El lavado con CSA y almacenado a 1 °C ó 4 °C proporcionó un producto de buena calidad después de 14 días. [ENG] Peas (Pisum sativum L.) are an important source of protein, carbohydrates, vitamins and minerals. Due to their very high respiration rate, loss of quality is fast. There is very little information about the optimal storage conditions for immature pea seeds, which are well adapted to be prepared as a minimally processed product. The objective of this research was to analyze the microbial quality and changes in vitamin C of fresh pea seeds stored at 1 and 4 °C in modified atmosphere packaging (MAP) (OPP 25 μm). Seeds were disinfected with sodium hypochlorite (HS) (100 ppm, pH 6.5) or with acidified sodium chlorite (CSA) (300 ppm, pH 1.8). After 14 days, the atmospheres within the packages were 8 kPa CO2/12 kPa O2 and 11 kPa CO2/10 kPa O2 at 1 and 4 °C, respectively. Mesophilic growth was reduced when samples were stored at 1 °C, regardless of the disinfectant, while enterobacteria were reduced in all the treatments. Vitamin C decreased from ≅ 700 mg kg-1 fw to 250 or 450 mg kg-1 fw, for HS and CSA respectively. Disinfection with CSA and storage at 1 or 4 °C allowed to obtain a good quality product after 14 days. Al Proyecto EUROLEGUME (EU FP7 Research Project No. 613781) financiado por fondos de la Unión Europea dentro del 7º Programa Marco de Investigación, Desarrollo Tecnológico y Diseminación.

Background. Some algae are an excellent sources of vitamin B12, of special interest for vegetarian/vegan consumers, and of fucose to supplement fruit and vegetable beverages such as smoothies. Nevertheless, supplementation of smoothies with algae may lead to possible quality changes during smoothie shelf life that need to be studied. Therefore, the quality changes in fresh green smoothies supplemented (2.2%) with nine edible algae (sea lettuce, kombu, wakame, thongweed, dulse, Irish moss, nori, Spirulina and Chlorella) were studied throughout 24 days at 5 °C. Results. The initial vitamin C content – 238.7–326.0 mg kg−1 fresh weight (FW) – of a 200 g portion of any of the smoothies ensured full coverage of its recommended daily intake, and still supplying 50–60% of the recommended intake after 7 days. Chlorella and Spirulina smoothies showed the highest vitamin B12 content (33.3 and 15.3 µg kg−1 FW, respectively), while brown algae showed fucose content of 141.1–571.3 mg kg−1 FW. These vitamin B12 and fucose contents were highly maintained during shelf life. Conclusion. The Spirulina supplementation of a 200 g smoothie portion ensured full coverage of the recommended vitamin B12 intake, with lower vitamin C degradation, during a shelf life of 17 days. Furthermore, thongweed and kombu are also considered as excellent fucose sources with similar shelf life. The authors are grateful to Spanish Ministry of Economy and Competitiveness, Project AGL2013−48830−C2−1−R and FEDER for financial support. We would also like to thank SAKATA SEED IBERICA S.L. for providing Bimi® samples.

Carrots are worldwide highly consumed due to their sensory characteristics and health-promoting properties. However, their low phenolic/antioxidant levels may be greatly increased with abiotic stresses. Accordingly, phenolic/antioxidant enhancements and related phenylalanine ammonia-lyase (PAL) activity of carrot shreds under different treatments (UVc radiation and hyperoxia conditions) were studied after a pre-enrichment incubation of 72 h at 15ºC. Subsequently, a carrot smoothie prepared from enriched carrot shreds was heat-treated (90ºC for 30 s) and stored at 5ºC up to 14 days. Heat-treated smoothies showed a good physicochemical and microbiological quality (

Thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. The effect of a pasteurization (P) treatment, 90 °C ± 2 ºC for 35 s, provided by continuous semi-industrial microwave (MW) under different con-ditions (high power/short time and low power/long time) or conventional pasteurization (CP) on orange-colored smoothies and their changes throughout 45 days of storage at 5 °C were investigated. Results indicated that vitamin C and antioxidant capacity (FRAP) in CP decreased dramatically in comparison with the unheated and MWP smoothies. On the contrary, all heating treatments increased the contents of total phenolic compounds (TPC) and carotenoids. Based on the sensory quality and microbial counts, the shelf life of all those heated smoothies reached 45 days. No Listeria monocytogenes growth was found and all microbial counts were below the European legal limits. MWP as compared to the CP method led to a greater reduction of mesophilic bacteria after 45 days at 5 °C (3.7 log cfu g-1 for CP and 1.6 log cfu g-1 for MWP). When highest power and shortest time MWP treatments were used (3,600 W for 93 s), FRAP and vitamin C were better preserved. This work was financially supported by MINECO-FEDER (AGL2013-48830-C2-1-R). Thanks are due to Instituto de Biotecnología Vegetal (IBV-UPCT) for providing some of the equipment

Phenolic compounds are phytochemicals with high health-promoting properties. Carrot is a vegetable highly worldwide consumed although its phenolic content is low compared to other plant products. The aim of this work was to evaluate changes in phenolic compounds in carrots caused by abiotic stresses. The phenylalanine ammonia-lyase (PAL) activity, phenolic compounds and total antioxidant capacity (TAC) changes during storage up to 72 h at 15 °C after wounding (shredding), 9 kJ UV-C m−2 pretreatment and hyperoxia (80 kPa) conditions of carrots were studied. Shredding and hyperoxia storage induced the highest phenolic compounds and TAC enhancements. Accumulation of phenolic compounds in shredded carrots could be structured in the following phases: 1st phase (

BACKGROUND Thermal processing causes a number of undesirable changes in physicochemical and bioactive properties of tomato products. Microwave (MW) technology is an emergent thermal industrial process that offers a rapid and uniform heating, high energy efficiency and high overall quality of the final product. The main quality changes of tomato puree after pasteurization at 96 ± 2 °C for 35 s, provided by a semi-industrial continuous microwave oven (MWP) under different doses (low power/long time to high power/short time) or by conventional method (CP) were studied. RESULTS All heat treatments reduced colour quality, total antioxidant capacity and vitamin C, with a greater reduction in CP than in MWP. On the other hand, use of an MWP, in particular high power/short time (1900 W/180 s, 2700 W/160 s and 3150 W/150 s) enhanced the viscosity and lycopene extraction and decreased the enzyme residual activity better than with CP samples. For tomato puree, polygalacturonase was the more thermo-resistant enzyme, and could be used as an indicator of pasteurization efficiency. CONCLUSION MWP was an excellent pasteurization technique that provided tomato puree with improved nutritional quality, reducing process times compared to the standard pasteurization process. © 2016 Society of Chemical Industry This work was financially supported by MINECO-FEDER (AGL2013-48830-C2-1-R).

A green fresh vegetables smoothie rich in health-promoting compounds was developed. Two thermal treatments to reduce microbial load and preserve quality were assayed: T1 (3 min at 80ºC) and T2 (45 s at 90ºC). Fresh blended unheated samples were used as control (CRL). The smoothie presented a viscoelastic behaviour. Thermal treatments reduced initial microbial loads by 1.3-2.4 and 1.4-3.1 log units, respectively. Samples were stored in darkness at 5 and 15ºC. Colour and physicochemical changes were reduced in thermal-treated samples throughout storage, which were better preserved at 5ºC rather than at 15ºC. Vitamin C changes during storage were fitted with a Weibullian distribution. Total vitamin C losses of T1 and T2 samples during storage at 15ºC were greatly reduced when they were stored at 5ºC. Initial total phenolics content (151.1 mg 29 kg-1 fw) was 44 and 36% increased after T1 and T2 treatments, respectively. The 3-p-30 coumaroyl quinic and chlorogenic acids accounted the 84.7% and 7.1% relative 31 abundance, respectively. Total antioxidant capacity (234.2 mg Trolox equivalent kg-1 fw) remained constant after the thermal treatments and was better maintained during storage in thermal-treated samples. Glucobrassicin accounted the 81% of the initial total glucosinolates content (117.8 mg kg-1 fw) of the smoothie. No glucosinolates losses were observed after T2 treatment being better preserved in thermal-treated samples. Conclusively, a short time–high temperature mild thermal treatment (T2) showed better quality and bioactive compounds retention in a green fresh vegetable smoothie during low temperature storage. The authors are grateful to Spanish Ministry of Economy and Competitiveness (MINECO) Project AGL2013-48830-C2-1-R and FEDER for financial support. We are grateful to M. Otón for his skilful technical assistance.

[SPA] Se ensayó diversas concentraciones de NaClO en el agua de lavado para preservar la calidad de semillas de haba mínimamente procesadas en fresco durante 7 días a 3ºC. Se lavó con agua de la red (testigo) y con diferentes concentraciones de hipoclorito (50 y 100 ppm) añadiendo en algunos casos ácido cítrico (200 ppm), pudiendo actuar como agente antipardeante o como potenciador de la acción del cloro. La duración de los tratamientos fue de 2 minutos y el tiempo de enjugado de 1 minuto, así como, la temperatura del agua fue en todos los casos de 5ºC. Debido a un mal diseño del envase, la AM generada en el interior no fue la adecuada, lo cual perjudicó la calidad de las habas. Sin embargo la calidad microbiológica de las mismas fue aceptable 7 días a 3ºC. Se necesitará optimizar primero el envase para sacar las conclusiones pertinentes en futuras experiencias. [ENG] Several NaClO concentrations in the wash water was tested to preserve the quality of minimally processed Vicia faba seeds stored for 7 days at 3 °C. Washing treatments were: tap water (control) and with several NaClO concentrations (50 and 100 ppm) adding in some cases citric acid (200 ppm) that may act as antibrowning agent or as an enhancer of the chlorine action. Samples were washed for 2 minutes and rinsed for 1 minute. The water temperature in all cases was 5 °C. Due to a wrong package design, the MA generated was inadequate, which induced quality deterioration of the seeds. However, the microbiological quality was still acceptable after 7 days at 3ºC. In further experiments the packaging will be needed to be optimized before drawing appropriate conclusions about NaClO dose optimization. Los autores agradecen a Frutas Esparza S.A. y al proyecto europeo EUROLEGUME FP7-BBBE.2013.1.2-02 la financiación recibida.

[SPA] Durante 3 años se estudió el efecto del riego deficitario controlado (RDC) sobre el contenido en vitamina C de la nectarina extra temprana “VioWhite 5” en cosecha, durante almacenamiento refrigerado (0ºC; 90-95% humedad relativa (HR) y tras simulación de comercialización (15ºC; 70-75% HR), centrándose en la relación con la enzima ascorbato peroxidasa (APX). Como estrategias de riego: i) Testigo, regado al 110% de la ETc (máxima evapotranspiración del cultivo); ii) RDC1, regado al 110% ETc durante los periodos críticos y al 85% del Testigo durante el resto de temporada; iii) RDC2, regado al 110% ETc durante los periodos críticos y al 80 y 60% Testigo durante la segunda etapa de crecimiento del fruto y en postcosecha, respectivamente. La respuesta del fruto sujeto a RDC mostró la existencia de estrés hídrico siendo dependiente de la intensidad y duración del RDC. Se observó que el comportamiento de la enzima APX y el contenido del ácido dehidroascórbico se podían utilizar como indicadores bioquímicos de estrés abiótico. La piel acumuló más vitamina C que la pulpa. Como conclusión, RDC es una práctica que permite el ahorro de agua (780 y 2.050 m3/ha año para RDC1 y RDC2, respectivamente) y mejora el contenido de vitamina C a través de la estimulación de APX. [ENG] During 3 consecutive years, the effect of regulated deficit irrigation (RDI) strategies on vitamin C at harvest, during cold storage (0ºC; 90-95% relative humidity (RH) and after retail sale period (15ºC; 70-75% RH) of extra early nectarine ‘VioWhite 5’ was studied, focusing on ascorbate peroxidase (APX) response. RDI strategies were programmed as: i) Control, irrigated at 110% of ETc (maximum crop evapotranspiration); ii) RDI1, irrigated at 110% ETc during critical periods of growth and at 85% of Control during the rest of growing season. iii) RDI2, irrigated at 110% ETc during critical periods of growth and at 80 and 60% Control during second fruit growth stage and late postharvest, respectively. The RDI application on extra early nectarines showed the existence of water stress on fruit response. This stress was dependent on RDI intensity. APX activity and dehydroascorbic acid levels revealed as good indicators of this abiotic stress. Peel metabolism was higher than pulp one and accumulated more vitamin C. Water savings were important: 780 and 2,050 m3/ha year for RDI1 and RDI2, respectively. As conclusion, RDI can be used as a water-reducing field practice demand while enhancing vitamin C through the activation of APX activity. Se agradece al Ministerio de Ciencia e Innovación-FEDER la financiación (proyecto AGL2010-19201-C04-02-AGR) y la beca predoctoral de N. Falagán, y al Instituto de Biotecnología Vegetal de la UPCT la utilización de algunos equipos.

[SPA] Se estudió el efecto de la desinfección con clorito sódico acidificado (CSA) (300 ppm) y agua ozonizada (O3) (0,6-0,7 ppm), individual o combinado, como alternativa a la desinfección con 100 ppm hipoclorito sódico (NaClO), sobre la calidad de dos variedades de brócoli (‘Bimi’ y ‘Parthenon’) mínimamente procesadas en fresco (MPF) durante 15 días a 4 ºC. Un lavado con agua corriente a 4 ºC fue considerado como control. Se estudió la evolución de la microflora alterante (aerobios mesófilos totales, enterobacterias, hongos y levaduras) y del contenido en compuestos bioactivos (capacidad antioxidante total, contenido en polifenoles totales, contenido de clorofilas y carotenos) durante la vida comercial. No se observaron diferencias en los principales géneros de microflora alterante entre los tratamientos alternativos y el NaClO ni en el día de procesado ni durante la vida comercial. Se produjo un aumento de la capacidad antioxidante total y del contenido de clorofilas y carotenos totales en ambas variedades durante la vida útil. El contenido de polifenoles totales se mantuvo constante durante los 15 días a 4 ºC. Como conclusión principal, una desinfección combinada de CSA + O3 mantuvo la calidad del brócoli MPF obteniendo valores similares al NaClO en todos los parámetros estudiados. [ENG] The effect of sanitizing with acidified sodium chlorite (ASC) (300 ppm) and ozonated water (O3) (0.6-0.7 ppm), along or combined, as an alternative to 100 ppm sodium hypochlorite (NaClO) on the quality during 15 days at 4 ºC of shelf-life of two minimally processed broccoli cvs. (‘Bimi’ and ‘Parthenon’) was studied. Washing with cold tap water (4 ºC) was used as control. The microbial quality (enterobacteria, mesophilic, yeasts and moulds) and the bioactive content (total antioxidant capacity, total phenolic content and chlorophyll and carotene content) were determined. There were no differences on the microbial load between treatments on the processing day and during the shelf-life. An increase in the total antioxidant capacity and in the chlorophyll and carotene content was shown after 15 days at 4 ºC. The total phenolics content did not change during the shelf life in both cvs. As main conclusion, a disinfection with a combination of CSA + O3 was effective for disinfect both varieties of FC broccoli showing similar results to NaClO in the studied parameters. A la empresa SAKATA SEEDS IBÉRICA S.L.U. por la financiación, y a la Cooperativa Agrícola de Producción SACOJE por proveer el material vegetal.

The effect of conventional and microwave pasteurization on the lycopene and β-carotene content and on the physicochemical quality attributes (TSS, pH, TA and color) of pasteurized orange smoothie were evaluated. The smoothie was elaborated with tomato, carrot, pumpkin, lemon juice, mineral water and marine salt. The conventional pasteurization (CP) was made by mean of a semi-industrial thermomix, while for microwave pasteurization (MWP) an innovative semi-industrial continuous microwave oven was used. After both pasteurization methods no significant changes in TSS, pH and TA were found while the carotenoids content was enhanced. MWP provided the highest lycopene and β-carotene content, showing MW equipment as a tool to pasteurize and improve the bioavailability of carotenoids of the smoothie. Thanks are due to Instituto de Biotecnología Vegetal (IBV-UPCT) for providing MW equipment. This work was financially supported by Spanish Ministry of Economy and competitiveness MINECO (AGL2013-48830-C2-1- R) and FEDER.