Author: "Arbovirus et Insectes Vecteurs" - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Arbovirus et Insectes Vecteurs"' showing total 148 results

Start Over Author "Arbovirus et Insectes Vecteurs"

148 results on '"Arbovirus et Insectes Vecteurs"'

1. Evidence of circulation of West Nile virus in Culex pipiens mosquitoes and horses in Morocco

Author: Maëlle Monier, Soukaina Arich, M’hammed Sarih, Cécile Beck, Laurence Mousson, Anna-Bella Failloux, Sara Moutailler, Khadija Akarid, Sylvie Lecollinet, Najlaa Assaid, Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP), Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Hassan II [Casablanca] (UH2MC), Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This study was funded by Institut Pasteur of Morocco and Institut Pasteur in Paris, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur [Paris] (IP), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0301 basic medicine, Serological technics, Veterinary (miscellaneous), viruses, 030231 tropical medicine, RT-PCR, Mosquito-borne-viruses, Enzyme-Linked Immunosorbent Assay, Virus, Disease Outbreaks, 03 medical and health sciences, 0302 clinical medicine, WNV, Seroepidemiologic Studies, Culex pipiens, medicine, Seroprevalence, Animals, Humans, Horses, biology, Meningoencephalitis, Outbreak, virus diseases, 030108 mycology & parasitology, biology.organism_classification, medicine.disease, Virology, 3. Good health, nervous system diseases, Flavivirus, Culex, Morocco, Infectious Diseases, Culicidae, Insect Science, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, biology.protein, Parasitology, Antibody, Usutu virus, West Nile virus, West Nile Fever
Abstract: International audience; West Nile virus (WNV) is one of the most widely distributed mosquito-borne viruses in the world. In North Africa, it causes human cases of meningoencephalitis with fatalities in Algeria and in Tunisia, whereas only horses were affected in Morocco. The aims of this study were to detect WNV in mosquitoes and to determine seroprevalence of WNV in Moroccan horses by the detection of IgG antibodies. A total of 1455 mosquitoes belonging to four different species were grouped by collection site, date, and sex with 10 specimens per pool and tested for 38 arboviruses using a high-throughput chip based on the BioMark Dynamic array system. Out of 146 mosquito pools tested, one pool was positive for WNV. This positive pool was confirmed by real time RT-PCR. The serosurvey showed that 33.7% (31/92) of horses were positive for competitive enzyme-linked im-munosorbent assay (cELISA) test. The flavivirus-sphere microsphere immnoassay (MIA) test, targeting three flaviviruses (WNV, Usutu virus (USUV) and Tick borne encephalitic virus (TBEV)) showed that 23 sera out of 31 were positive for WNV, two for USUV, two for USUV or WNV, and four for an undetermined flavivirus. Virus neutralization tests with USUV and WNV showed that 28 of 31 sera were positive for WNV and all sera were negative for USUV. This study reports, for the first time, the detection of WNV from Culex pipiens mosquitoes in Morocco and its circulation among horses. This highlights that the detection of arboviruses in mosquitoes could serve as an early warning signal of a viral activity to prevent future outbreaks in animals and humans.
Published: 2020
Full Text: View/download PDF

2. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Author: Yvon Perrin, Mawlouth Diallo, Frédéric Jourdain, Amandine Guidez, Xavier de Lamballerie, Marie Vazeille, Isabelle Dusfour, Anubis Vega-Rúa, Davy Jiolle, Johana Restrepo, Lena Yousfi, Géraldine Piorkowski, Fabrice Chandre, Alioune Gaye, Filipe Vieira Santos de Abreu, Sara Moutailler, Anna-Bella Failloux, Merril Wongsokarijo, Tessa M. Visser, Sandrine Chantilly, Constantianus J. M. Koenraadt, Diawo Diallo, Laurence Mousson, Christophe Paupy, Taissa Pereira dos Santos, Sébastien Boyer, Ricardo Lourenço de Oliveira, Arnaud Cannet, Veasna Duong, Elodie Devillers, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Centre national d'expertise des vecteurs, Montpellier (CNEV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Collectivité Territoriale de Guyane (CTG), Institut Pasteur de la Guyane, Fundação Oswaldo Cruz (FIOCRUZ), Wageningen University and Research Centre [Wageningen] (WUR), Public Health Central Laboratory of Suriname, Public health, Institut Pasteur de Dakar, Institut Pasteur du Cambodge, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under ZIKAlliance Grant Agreement no. 734548. The project also received funding from the 2014 PTR Anses-Institut Pasteur project (N° 511) for CHIPARBO, and the European Union’s Horizon 2020 Research and Innovation Programme under EVAg Grant Agreement no. 653316, and from an 'Investissement d’Avenir' ‘grant from the Agence Nationale de la Recherche (CEBA ANR-10-LABX-2501 grant) supporting the project TIGERAMAZON.. The French General Directorate of Health funded the work performed in Guadeloupe and French Guiana. The Programme Opérationnel FEDER-Guadeloupe-Conseil Régional 2014–2020 (grant 2015-FED-192) supported the researchers from Guadeloupe., We thank Christelle Delannay and the staff of the Regional Health Agency of Guadeloupe for their support during the sampling campaigns, ANR-10-LABX-25-01/10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), European Project: 734548,ZIKAlliance(2016), European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Vector Control Group (MIVEGEC-VCG), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Wageningen University and Research [Wageningen] (WUR), ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Pasteur [Paris] (IP), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Subjects: Male, Endemic Diseases, mosquito-borne viruses, [SDV]Life Sciences [q-bio], viruses, lcsh:QR1-502, Pilot Projects, Disease Vectors, molecular epidemiology, lcsh:Microbiology, Zika virus, Human health, Pandemic, Laboratory of Entomology, Guadeloupe, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Suriname, biology, Zika Virus Infection, virus diseases, PE&RC, Senegal, French Guiana, 3. Good health, Epidemiological Monitoring, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, surveillance, RNA, Viral, Enzootic, Female, Cambodia, Brazil, Context (language use), Mosquito Vectors, Arbovirus Infections, Arbovirus, Article, 03 medical and health sciences, parasitic diseases, medicine, Animals, Humans, Epidemics, 030304 developmental biology, 030306 microbiology, fungi, Zika Virus, Mosquito-borne viruses, biology.organism_classification, medicine.disease, Laboratorium voor Entomologie, Virology, High-Throughput Screening Assays, Culicidae, Vector (epidemiology), microfluidic analysis, Arboviruses
Abstract: Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark&trade, Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.
Published: 2019
Full Text: View/download PDF

3. Evolution and biological significance of flaviviral elements in the genome of the arboviral vector Aedes albopictus

Author: Mariangela Bonizzoni, Catherine Dauga, Michele Marconcini, Pei-Shi Yen, Xavier de Lamballerie, Gaelle Gabiane, Yoann Madec, Vincent Houé, Laurence Mousson, Anna-Bella Failloux, Marie Suez, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Sorbonne Université (SU), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Università degli Studi di Pavia, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Unité des Virus Emergents (UVE), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), This study was supported by the Institut Pasteur, the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant number ANR-10-LABX-62-IBEID to A-BF), the European Union’s Horizon 2020 research and innovation program, ZIKAlliance program under ZIKAlliance grant agreement no. 734548 to A-BF, and the European Research Council, NIRV_HOST_INT Council (grant agreement number 682394 – NIRV_HOST_INT to VH and MB)., We are grateful to Basile Kamgang for providing mosquito samples, and Dominique Higuet, Anna Malacrida, Giuliano Gasperi for discussions. We also thank Charlotte Balière, Valérie Caro and Aurélia Kwasiborski for help in genotyping mosquitoes., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 682394,H2020,ERC-2015-CoG,NIRV_HOST_INT(2016), European Project: 734548,ZIKAlliance(2016), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Università degli Studi di Pavia = University of Pavia (UNIPV), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Arbovirus et Insectes Vecteurs, Epidémiologie des Maladies Emergentes, Pasteur-Cnam risques infectieux et émergents (PACRI), Università di Pavia, Emergence des Pathologies Virales (EPV), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), DEMESLAY GOUGAM, MARIE, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Population genomics of co-evolution between non-retroviral RNA viruses and their hosts - NIRV_HOST_INT - - H20202016-05-01 - 2021-04-30 - 682394 - VALID, and A global alliance for Zika virus control and prevention - ZIKAlliance - 2016-10-01 - 2019-09-30 - 734548 - VALID
Subjects: Aedes albopictus, Epidemiology, viruses, education, 030231 tropical medicine, Immunology, Dengue virus, medicine.disease_cause, Microbiology, Genome, NIRVS, arboviral diseases, 03 medical and health sciences, 0302 clinical medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Virology, Drug Discovery, medicine, genetic structure, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Aedes, 0303 health sciences, vector competence, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], biology, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], fungi, virus diseases, General Medicine, biology.organism_classification, Infectious Diseases, Biological significance, Evolutionary biology, Vector (epidemiology), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Parasitology
Abstract: Since its genome details are publically available, the mosquito Aedes albopictus has become the central stage of attention for deciphering multiple biological and evolutionary aspects at the root of its success as an invasive species. Its genome of 1,967 Mb harbours an unusual high number of non-retroviral integrated RNA virus sequences (NIRVS). NIRVS are enriched in piRNA clusters and produce piRNAs, suggesting an antiviral effect. Here, we investigated the evolutionary history of NIRVS in geographically distant Ae. albopictus populations by comparing genetic variation as derived by neutral microsatellite loci and seven selected NIRVS. We found that the evolution of NIRVS was far to be neutral with variations both in their distribution and sequence polymorphism among Ae. albopictus populations. The Flaviviral elements AlbFlavi2 and AlbFlavi36 were more deeply investigated in their association with dissemination rates of dengue virus (DENV) and chikungunya virus (CHIKV) in Ae. albopictus at both population and individual levels. Our results show a complex association between NIRVS and DENV/CHIKV opening a new avenue for investigating the functional role of NIRVS as antiviral elements shaping vector competence of mosquitoes to arboviruses.
Published: 2019
Full Text: View/download PDF

4. GloPID-R report on Chikungunya, O'nyong-nyong and Mayaro virus, part I: Biological diagnostics

Author: A. J. Rodriguez-Morales, M. G. Rosa-Freitas, Guilherme S. Ribeiro, Nikos Vasilakis, Lisa F. P. Ng, Mariama Dalanda Diallo, Scott C. Weaver, Patrícia Brasil, Jan Felix Drexler, Johan Neyts, Anna-Bella Failloux, François Simon, Alain Kohl, A. Vega Rua, Maria Rios, Michael P. Busch, Michael S. Diamond, André Siqueira, Michael A. Drebot, P. Gallian, Sébastien Boyer, Ricardo Lourenço-de-Oliveira, Chantal Reusken, Angelle Desiree LaBeaud, Thomas Jaenisch, Marc Lecuit, Laura Pezzi, X. de Lamballerie, Graham Simmons, Amadou A. Sall, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Virologie [UNIV Corse-Inserm] (EA7310), Université Pascal Paoli (UPP)-Institut National de la Santé et de la Recherche Médicale (INSERM), National Institute for Public Health and the Environment [Bilthoven] (RIVM), Erasmus University Medical Center [Rotterdam] (Erasmus MC), The University of Texas Medical Branch (UTMB), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Humboldt University Of Berlin, Berlin Institute of Health (BIH), German Centre for Infection Research (DZIF), University of California [San Francisco] (UC San Francisco), University of California (UC), Stanford School of Medicine [Stanford], Stanford Medicine, Stanford University-Stanford University, Washington University in Saint Louis (WUSTL), Public Health Agency of Canada, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Universidade Federal da Bahia (UFBA), University of Glasgow, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Agency for science, technology and research [Singapore] (A*STAR), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, Institut Pasteur du Cambodge, Institut Pasteur de Dakar, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Heidelberg University Hospital [Heidelberg], Rega Institute for Medical Research [Leuven, België], Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), U.S. Food and Drug Administration (FDA), Universidad Tecnológica de Pereira [Colombie] (UTP), Hôpital d'Instruction des Armées Laveran, Service de Santé des Armées, Institut Pasteur de la Guadeloupe, Publication fees were covered by the Global Research Collaboration for Infectious Disease Preparedness (GloPID-R)., Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pascal Paoli (UPP), Humboldt-Universität zu Berlin, University of California [San Francisco] (UCSF), University of California, Fundação Oswaldo Cruz (FIOCRUZ), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Virology, Charité - Universitätsmedizin Berlin / Charite - University Medicine Berlin, Humboldt Universität zu Berlin, Stanford University School of Medicine [Stanford], Stanford University [Stanford], Washington University in St Louis, Federal University of Bahia, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Arbovirus et Insectes Vecteurs, and Rega Institute for Medical Research
Subjects: 0301 basic medicine, medicine.medical_specialty, Genes, Viral, 030106 microbiology, Disease, Alphavirus, Mosquito Vectors, Cross Reactions, medicine.disease_cause, Antibodies, Viral, Communicable Diseases, Emerging, Virus, 03 medical and health sciences, Seroepidemiologic Studies, Virology, O'nyong-nyong, Epidemiology, medicine, Animals, Humans, O'nyong-nyong Virus, Chikungunya, Pathology, Molecular, Diagnostics, Phylogeny, Pharmacology, biology, business.industry, Alphavirus Infections, Outbreak, biology.organism_classification, Mayaro virus, 3. Good health, 030104 developmental biology, Cryoglobulinemia, Infectious disease (medical specialty), Preparedness, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Chikungunya Fever, business, Chikungunya virus
Abstract: The GloPID-R (Global Research Collaboration for Infectious Disease Preparedness) Chikungunya (CHIKV), O'nyong-nyong (ONNV) and Mayaro virus (MAYV) Working Group is investigating the natural history, epidemiology and medical management of infection by these viruses, to identify knowledge gaps and to propose recommendations for direct future investigations and rectification measures. Here, we present the first report dedicated to diagnostic aspects of CHIKV, ONNV and MAYV. Regarding diagnosis of the disease at the acute phase, molecular assays previously described for the three viruses require further evaluation, standardized protocols and the availability of international standards representing the genetic diversity of the viruses. Detection of specific IgM would benefit from further investigations to clarify the extent of cross-reactivity among the three viruses, the sensitivity of the assays, and the possible interfering role of cryoglobulinaemia. Implementation of reference panels and external quality assessments for both molecular and serological assays is necessary. Regarding sero-epidemiological studies, there is no reported high-throughput assay that can distinguish among these different viruses in areas of potential co-circulation. New specific tools and/or improved standardized protocols are needed to enable large-scale epidemiological studies of public health relevance to be performed. Considering the high risk of future CHIKV, MAYV and ONNV outbreaks, the Working Group recommends that a major investigation should be initiated to fill the existing diagnostic gaps. ispartof: ANTIVIRAL RESEARCH vol:166 pages:66-81 ispartof: location:Netherlands status: published
Published: 2019
Full Text: View/download PDF

5. Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016-2018

Author: Aloísio Falqueto, Maria Ignez Lima Bersot, Monique de Albuquerque Motta, Anna-Bella Failloux, Ieda Pereira Ribeiro, Mario Sergio Ribeiro, Eduardo Oyama Lins Fonseca, Alessandro Pecego Martins Romano, Iule de Souza Bonelly, Mariela Martínez Gómez, Ricardo Lourenço-de-Oliveira, José Luis da Silva, Taissa Pereira dos Santos, Myrna C. Bonaldo, Rafaella Moraes de Miranda, Roberto da Costa Laperrière, Rodrigo Fabiano do Carmo Said, Christophe Paupy, Márcia Gonçalves de Castro, Anielly Ferreira-de-Brito, Sara Moutailler, Marcelo Quintela Gomes, Alexandre Dos Santos, Roberta Gomes Carvalho, Filipe Vieira Santos de Abreu, Maycon Sebastião Alberto Santos Neves, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Instituto Federal do Norte de Minas Gerais (IFNMG), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ministério da Saúde [Brasília, Brazil], Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Acknowledgments to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Institut Pasteur - Transversal Research Program for funding this work., Fundação Oswaldo Cruz (FIOCRUZ), Institut Pasteur [Paris], Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Lourenco-de-Oliveira, Ricardo, Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Arbovirus et Insectes Vecteurs, École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Subjects: 0301 basic medicine, Male, Aedes albopictus, Epidemiology, [SDV]Life Sciences [q-bio], 030106 microbiology, Immunology, Population Dynamics, education, Zoology, Aedes aegypti, Mosquito Vectors, Biology, Microbiology, sabethes, aedes, haemagogus, atlantic forest, yellow fever, Article, law.invention, Disease Outbreaks, 03 medical and health sciences, Abundance (ecology), law, Aedes, Virology, Drug Discovery, medicine, Animals, Haemagogus, Cities, ComputingMilieux_MISCELLANEOUS, Yellow fever, Outbreak, General Medicine, medicine.disease, biology.organism_classification, 3. Good health, Phylogeography, 030104 developmental biology, Infectious Diseases, Transmission (mechanics), Sabethes, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Parasitology, Female, Atlantic forest, Yellow fever virus, Brazil
Abstract: International audience; The yellow fever virus (YFV) caused a severe outbreak in Brazil in 2016-2018 that rapidly spread across the Atlantic Forest in its most populated region without viral circulation for almost 80 years. A comprehensive entomological survey combining analysis of distribution, abundance and YFV natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five Brazilian states. In total, 17,662 mosquitoes of 89 species were collected. Before evidence of virus circulation, mosquitoes were tested negative but traditional vectors were alarmingly detected in 82% of municipalities, revealing high receptivity to sylvatic transmission. During the outbreak, five species were found positive in 42% of municipalities. Haemagogus janthinomys and Hg. leucocelaenus are considered the primary vectors due to their large distribution combined with high abundance and natural infection rates, concurring together for the rapid spread and severity of this outbreak. Aedes taeniorhynchus was found infected for the first time, but like Sabethes chloropterus and Aedes scapularis, it appears to have a potential local or secondary role because of their low abundance, distribution and infection rates. There was no evidence of YFV transmission by Aedes albopictus and Aedes aegypti, although the former was the most widespread species across affected municipalities, presenting an important overlap between the niches of the sylvatic vectors and the anthropic ones. The definition of receptive areas, expansion of vaccination in the most affected age group and exposed populations and the adoption of universal vaccination to the entire Brazilian population need to be urgently implemented.
Published: 2019
Full Text: View/download PDF

6. Insecticide resistance genes affect Culex quinquefasciatus vector competence for West Nile virus

Author: Mawlouth Diallo, Mylène Weill, Laurence Mousson, Haoues Alout, Célestine M. Atyame, Marie Vazeille, Anna-Bella Failloux, Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), This study was supported by the AXA research fund and the Pasteur-Cantarini postdoctoral fellowships. H.A. is supported by Marie Skołodowska-Curie under the grant agreement number 749897., We are very grateful to Sandra Unal, Patrick Makoundou and Jocelyne Alexandre for technical assistance., Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Arbovirus et Insectes Vecteurs, and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)
Subjects: Pesticide resistance, West Nile virus, viruses, 030231 tropical medicine, Genes, Insect, Mosquito Vectors, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, parasitic diseases, medicine, Animals, genetics, Gene, 030304 developmental biology, General Environmental Science, 0303 health sciences, vector competence, General Immunology and Microbiology, biology, Ecology, virus diseases, Culex quinquefasciatus, General Medicine, Feeding Behavior, Subject Category: Ecology Subject Areas: ecology, insecticide resistance, Pesticide, biology.organism_classification, Virology, 3. Good health, Culex, Insecticide resistance, microbiology Keywords: insecticide resistance, arboviruses, Vector (epidemiology), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, General Agricultural and Biological Sciences, Disease transmission, West Nile Fever
Abstract: Insecticide resistance has been reported to impact the interactions between mosquitoes and the pathogens they transmit. However, the effect on vector competence for arboviruses still remained to be investigated. We examined the influence of two insecticide resistance mechanisms on vector competence of the mosquito Culex quinquefasciatus for two arboviruses, Rift Valley Fever virus (RVFV) and West Nile virus (WNV). Three Cx. quinquefasciatus lines sharing a common genetic background were used: two insecticide-resistant lines, one homozygous for amplification of the Ester 2 locus (SA2), the other homozygous for the acetylcholinesterase ace-1 G119S mutation (SR) and the insecticide-susceptible reference line Slab. Statistical analyses revealed no significant effect of insecticide-resistant mechanisms on vector competence for RVFV. However, both insecticide resistance mechanisms significantly influenced the outcome of WNV infections by increasing the dissemination of WNV in the mosquito body, therefore leading to an increase in transmission efficiency by resistant mosquitoes. These results showed that insecticide resistance mechanisms enhanced vector competence for WNV and may have a significant impact on transmission dynamics of arboviruses. Our findings highlight the importance of understanding the impacts of insecticide resistance on the vectorial capacity parameters to assess the overall consequence on transmission.
Published: 2019
Full Text: View/download PDF

7. Synthetic miRNAs induce dual arboviral-resistance phenotypes in the vector mosquito Aedes aegypti

Author: Yen, Pei-Shi, James, Anthony, Li, Jian-Chiuan, Chen, Chun-Hong, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Departments of Microbiology & Molecular Genetics and Molecular Biology & Biochemistry, University of California [Irvine] (UCI), University of California-University of California, National Health Research Institutes [Taiwan] (NHRI), This study was funded by the Institut Pasteur and the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (Grant No. ANR-10-LABX-62-IBEID) and the Ministry of Science and Technology, Taiwan (Grant No. MOST 105-2325-B-400-006, MOHW105-TDU-M-212-000006). P.-S.Y. was supported by the Pasteur-Paris University (PPU) program., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Arbovirus et Insectes Vecteurs, University of California [Irvine] ( UCI ), National Health Research Institutes [Taiwan] ( NHRI ), ANR-10-LABX-0062/10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases ( 2010 ), Institut Pasteur [Paris] (IP), University of California [Irvine] (UC Irvine), and University of California (UC)-University of California (UC)
Subjects: [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, lcsh:Biology (General), viruses, fungi, parasitic diseases, virus diseases, Alphavirus, Dengue Virus, Virus-host interactions, lcsh:QH301-705.5, [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Abstract: International audience; Mosquito-borne arboviruses are responsible for recent dengue, chikungunya, and Zika pandemics. The yellow-fever mosquito, Aedes aegypti, plays an important role in the transmission of all three viruses. We developed a miRNA-based approach that results in a dual resistance phenotype in mosquitoes to dengue serotype 3 (DENV-3) and chikungunya (CHIKV) viruses. The target viruses are from two distinct arboviral families and the antiviral mechanism is designed to function through the endogenous miRNA pathway in infected mosquitoes. Challenge experiments showed reductions in viral transmission efficiency of transgenic mosquitoes. Several components of mosquito fitness were examined, and transgenic mosquitoes with the PUb promoter showed minor fitness costs at all developing stages. Further development of these strains with gene editing tools could make them candidates for releases in population replacement strategies for sustainable control of multiple arbovirus diseases.
Published: 2018
Full Text: View/download PDF

8. Zoonotic aspects of vector-borne infections

Author: Failloux, Anna-Bella, Moutailler, Sarah, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), This article would never have seen the light of day without the financial support of the Institut Pasteur and the French Agency for Food, Environmental and Occupational Health and Safety (ANSES), through a cross-disciplinary research project (PTR-ChipArbo). They would also like to thank the European Network for Neglected Vectors and Vector-Borne Infections (COST Action TD1303), as well as the Ticks and Tick- Borne Diseases Working Group of the Réseau Ecologie des Interactions Durables at the French National Institute for Agricultural Research (INRA)., The authors wish to thank all members of the Research and Expertise Unit ‘Arboviruses and Insect Vectors’ and the members of the Vectotiq team. Particular thanks go to Dr Sarah Bonnet and Dr Muriel Vayssier for their advice and helpful discussions., DEMESLAY GOUGAM, MARIE, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Pasteur [Paris] (IP), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, and Arbovirus et Insectes Vecteurs
Subjects: [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Humans, Urban Population, Disease Vectors, zoonosis, MESH: Disease Vectors, Communicable Diseases, epidemic, MESH: Urban Population, Zoonoses, arthropod, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Communicable Diseases, Animals, Humans, emergence, MESH: Animals, MESH: Zoonoses, vector
Abstract: http://web.oie.int/boutique/index.php?page=ficprod&id_prec=1407&id_produit=1516&lang=fr&fichrech=1; International audience; Vector-borne diseases are principally zoonotic diseases transmitted to humans by animals. Pathogens such as bacteria, parasites and viruses are primarily maintained within an enzootic cycle between populations of non-human primates or other mammals and largely non-anthropophilic vectors. This 'wild' cycle sometimes spills over in the form of occasional infections of humans and domestic animals. Lifestyle changes, incursions by humans into natural habitats and changes in agropastoral practices create opportunities that make the borders between wildlife and humans more permeable. Some vector-borne diseases have dispensed with the need for amplification in wild or domestic animals and they can now be directly transmitted to humans. This applies to some viruses (dengue and chikungunya) that have caused major epidemics. Bacteria of the genus Bartonella have reduced their transmission cycle to the minimum, with humans acting as reservoir, amplifier and disseminator. The design of control strategies for vector-borne diseases should be guided by research into emergence mechanisms in order to understand how a wild cycle can produce a pathogen that goes on to cause devastating urban epidemics.
Published: 2015

9. Climate change and vector-borne diseases: a multi-omics approach of temperature-induced changes in the mosquito

Author: Bellone, Rachel, Lechat, Pierre, Mousson, Laurence, Gilbart, Valentine, Piorkowski, Géraldine, Bohers, Chloé, Merits, Andres, Kornobis, Etienne, Reveillaud, Julie, Paupy, Christophe, Vazeille, Marie, Martinet, Jean-Philippe, Madec, Yoann, de Lamballerie, Xavier, Dauga, Catherine, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Collège Doctoral, Sorbonne Université (SU), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Tartu, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Université Paris Cité (UPCité)-Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Paris Cité (UPCité)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), This work was supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 731060 (Infravec2, Research Infrastructures for the control of vector-borne diseases), the Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant n°ANR-10-LABX-62-IBEID) and the European Union’s Horizon 2020 research and innovation programme under ZIKAlliance grant agreement No 734548., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and European Project: 734548,ZIKAlliance(2016)
Subjects: chikungunya, [SDV]Life Sciences [q-bio], Temperature, General Medicine, Aedes albopictus
Abstract: Background Climate change and globalization contribute to the expansion of mosquito vectors and their associated pathogens. Long spared, temperate regions have had to deal with the emergence of arboviruses traditionally confined to tropical regions. Chikungunya virus (CHIKV) was reported for the first time in Europe in 2007, causing a localized outbreak in Italy, which then recurred repeatedly over the years in other European localities. This raises the question of climate effects, particularly temperature, on the dynamics of vector-borne viruses. The objective of this study is to improve the understanding of the molecular mechanisms set up in the vector in response to temperature. Methods We combine three complementary approaches by examining Aedes albopictus mosquito gene expression (transcriptomics), bacterial flora (metagenomics) and CHIKV evolutionary dynamics (genomics) induced by viral infection and temperature changes. Results We show that temperature alters profoundly mosquito gene expression, bacterial microbiome and viral population diversity. We observe that (i) CHIKV infection upregulated most genes (mainly in immune and stress-related pathways) at 20°C but not at 28°C, (ii) CHIKV infection significantly increased the abundance of Enterobacteriaceae Serratia marcescens at 28°C and (iii) CHIKV evolutionary dynamics were different according to temperature. Conclusion The substantial changes detected in the vectorial system (the vector and its bacterial microbiota, and the arbovirus) lead to temperature-specific adjustments to reach the ultimate goal of arbovirus transmission; at 20°C and 28°C, the Asian tiger mosquito Ae. albopictus was able to transmit CHIKV at the same efficiency. Therefore, CHIKV is likely to continue its expansion in the northern regions and could become a public health problem in more countries than those already affected in Europe.
Published: 2023
Full Text: View/download PDF

10. Determinants of Chikungunya and O’nyong-Nyong Virus Specificity for Infection of Aedes and Anopheles Mosquito Vectors

Author: Solène Cottis, Adrien A. Blisnick, Anna-Bella Failloux, Kenneth D. Vernick, Génétique et Génomique des Insectes Vecteurs - Genetics and Genomics of Insect Vectors, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Ecole Doctorale Complexité du Vivant (ED515), Sorbonne Université (SU), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), This work received financial support to KDV from the Agence Nationale de la Recherche, #ANR-19-CE35-0004 ArboVec, National Institutes of Health, NIAID #AI145999, and French Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' #ANR-10-LABX-62-IBEID, and to ABF from the Agence Nationale de la Recherche, #ANR-19-CE35-0004 ArboVec, and French Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' #ANR-10-LABX-62-IBEID., ANR-19-CE35-0004,ArboVEC,Barrières d'hôtes dans la spécificité des interactions entre moustiques vecteurs et arbovirus(2019), and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Subjects: chikungunya virus, Infectious Diseases, Aedes, Virology, [SDV]Life Sciences [q-bio], Anopheles, vector specificity, host-pathogen interactions, o'nyong-nyong virus
Abstract: International audience; Mosquito-borne diseases caused by viruses and parasites are responsible for more than 700 million infections each year. Anopheles and Aedes are the two major vectors for, respectively, malaria and arboviruses. Anopheles mosquitoes are the primary vector of just one known arbovirus, the alphavirus o’nyong-nyong virus (ONNV), which is closely related to the chikungunya virus (CHIKV), vectored by Aedes mosquitoes. However, Anopheles harbor a complex natural virome of RNA viruses, and a number of pathogenic arboviruses have been isolated from Anopheles mosquitoes in nature. CHIKV and ONNV are in the same antigenic group, the Semliki Forest virus complex, are difficult to distinguish via immunodiagnostic assay, and symptomatically cause essentially the same human disease. The major difference between the arboviruses appears to be their differential use of mosquito vectors. The mechanisms governing this vector specificity are poorly understood. Here, we summarize intrinsic and extrinsic factors that could be associated with vector specificity by these viruses. We highlight the complexity and multifactorial aspect of vectorial specificity of the two alphaviruses, and evaluate the level of risk of vector shift by ONNV or CHIKV.
Published: 2023
Full Text: View/download PDF

11. Assessing vector competence of mosquitoes from northeastern France to West Nile virus and Usutu virus

Author: Jean-Philippe Martinet, Chloé Bohers, Marie Vazeille, Hubert Ferté, Laurence Mousson, Bruno Mathieu, Jérôme Depaquit, Anna-Bella Failloux, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut de Parasitologie et de Pathologie Tropicale (IPPTS), Université de Strasbourg (UNISTRA), Centre Hospitalier Universitaire de Reims (CHU Reims), This study was funded by the ANSES APR-EST Grant N° 2020/01/129 (JPM, JD, BM, ABF) and the Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID) (ABF)., and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Subjects: [SDV]Life Sciences [q-bio]
Abstract: West Nile virus (WNV) and Usutu virus (USUV) are two arthropod-borne viruses that circulate in mainland France. Assessing vector competence has only been conducted so far with mosquitoes from southern France while an increasingly active circulation of WNV and USUV has been reported in the last years. The main vectors are mosquitoes of theCulexgenus and the common mosquitoCulex pipiens. Here, we measure the vector competence of five mosquito species (Aedes rusticus, Aedes albopictus, Anopheles plumbeus, Culex pipiensandCuliseta longiareolata) present in northeastern France. Field-collected populations were exposed to artificial infectious blood meal containing WNV or USUV and examined at different days post-infection. We show that (i)Cx. pipienstransmitted WNV and USUV, (ii)Ae. rusticusonly WNV, and (iii) unexpectedly,Ae. albopictustransmitted both WNV and USUV. Less surprising,An. plumbeuswas not competent for both viruses. Combined with data on distribution and population dynamics, these assessments of vector competence will help in developing a risk map and implementing appropriate prevention and control measures.Author summaryWest Nile virus (WNV) and Usutu virus (USUV) are on the rise in Europe and in France. WNV is reported in France as early as the 1960s in the Camargue and USUV more recently, in 2015 in eastern France. The re-emergence of WNV infections in the Camargue is associated with an expansion towards the North which is also favorable to maintain a viral transmission cycle. USUV frequently co-circulates with WNV sharing the same mosquito vectors.Culex pipiens, able to feed on birds and humans, is considered to be the main vector in France. Our study is the first to investigate the vector competence to WNV and USUV of five different mosquito species collected in northeastern France. We ascertain that FrenchCx. pipiensmosquitoes are competent to both WNV and USUV. More surprisingly, the mosquitoAedes albopictusfrom northeastern France was able to transmit WNV and USUV. Based on our result, we propose that surveillance of mosquitoes combined with viral detections must be implemented in northeastern France to allow early viral detection and timely intervention to prevent outbreaks of these two neurological diseases.
Published: 2023
Full Text: View/download PDF

12. The Native Wolbachia Symbionts Limit Transmission of Dengue Virus in Aedes albopictus

Author: Karima Zouache, Anna-Bella Failloux, Laurence Mousson, Vincent Raquin, Patrick Mavingui, Camilo Arias-Goeta, Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), This work was funded by the ACIP A-10-2009 (Institut Pasteur), the European Commission Seventh Framework Program 'INFRAvec' (grant number 228421) and the French Government's Investissement d'Avenir program, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant number ANR-10-LABX-62-IBEID). LM was supported by the ACIP A-10-2009 (Institut Pasteur). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), European Project: 228421,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,INFRAVEC(2009), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)
Subjects: moustique, chikungunya, viruses, Dengue virus, Disease Vectors, medicine.disease_cause, MESH: Bacterial Load, MESH: Dengue Virus, Virus Replication, Dengue fever, Mice, 0302 clinical medicine, réplication virale, Aedes, MESH: Animals, Chikungunya, 0303 health sciences, education.field_of_study, MESH: Symbiosis, biology, MESH: Real-Time Polymerase Chain Reaction, lcsh:Public aspects of medicine, virus diseases, MESH: Aedes, Viral Load, 3. Good health, extraction de l'arn, Host-Pathogen Interaction, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Wolbachia, Wolbachia, Female, MESH: Animal Structures, MESH: Reunion, MESH: Viral Load, Research Article, Aedes albopictus, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, 030231 tropical medicine, Population, Aedes aegypti, MESH: Disease Vectors, Real-Time Polymerase Chain Reaction, Microbiology, Vector Biology, Microbial Ecology, 03 medical and health sciences, parasitic diseases, medicine, Animals, Humans, MESH: Saliva, education, Saliva, Symbiosis, salive, MESH: Mice, Biology, 030304 developmental biology, MESH: Humans, glande salivaire, MESH: Virus Replication, fungi, Public Health, Environmental and Occupational Health, Animal Structures, lcsh:RA1-1270, biochemical phenomena, metabolism, and nutrition, Dengue Virus, biology.organism_classification, medicine.disease, Virology, dengue, Bacterial Load, MESH: Microbial Interactions, Emerging Infectious Diseases, Microbial Interactions, MESH: Female, Zoology, Entomology, Reunion
Abstract: Background The chikungunya (CHIK) outbreak that struck La Reunion Island in 2005 was preceded by few human cases of Dengue (DEN), but which surprisingly did not lead to an epidemic as might have been expected in a non-immune population. Both arboviral diseases are transmitted to humans by two main mosquito species, Aedes aegypti and Aedes albopictus. In the absence of the former, Ae. albopictus was the only species responsible for viral transmission on La Reunion Island. This mosquito is naturally super-infected with two Wolbachia strains, wAlbA and wAlbB. While Wolbachia does not affect replication of CHIK virus (CHIKV) in Ae. albopictus, a similar effect was not observed with DEN virus (DENV). Methods/Principal Findings To understand the weak vectorial status of Ae. albopictus towards DENV, we used experimental oral infections of mosquitoes from La Reunion Island to characterize the impact of Wolbachia on DENV infection. Viral loads and Wolbachia densities were measured by quantitative PCR in different organs of Ae. albopictus where DENV replication takes place after ingestion. We found that: (i) Wolbachia does not affect viral replication, (ii) Wolbachia restricts viral density in salivary glands, and (iii) Wolbachia limits transmission of DENV, as infectious viral particles were only detected in the saliva of Wolbachia-uninfected Ae. albopictus, 14 days after the infectious blood-meal. Conclusions We show that Wolbachia does not affect the replication of DENV in Ae. albopictus. However, Wolbachia is able to reduce viral infection of salivary glands and limit transmission, suggesting a role of Wolbachia in naturally restricting the transmission of DENV in Ae. albopictus from La Reunion Island. The extension of this conclusion to other Ae. albopictus populations should be investigated., Author Summary Aedes albopictus is an invasive species that is expanding its natural range of geographic distribution. While it was previously considered a secondary vector of different arboviruses, this mosquito species is involved in the most recent outbreaks of chikungunya but contributes weakly to dengue outbreaks. Ae. albopictus naturally carries two strains of the bacterium Wolbachia, wAlbA and wAlbB. Present in 20% of insect species, Wolbachia is an obligate intracellular symbiont mainly transmitted through females. When inoculated into some mosquito hosts, Wolbachia is able to shorten the adult life span and to block arbovirus transmission. We have previously shown that Wolbachia is not capable of limiting chikungunya replication in the mosquito vector. In this study, we show that the native Wolbachia is able to limit dengue transmission by restricting the delivery of infectious viral particles from the mosquito saliva when biting. Therefore, our results might explain the low vector competence of Ae. albopictus for dengue, and thus its weak contribution as an epidemic dengue vector.
Published: 2012
Full Text: View/download PDF

13. Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector

Author: Karima, Zouache, Rory J, Michelland, Anna-Bella, Failloux, Genevieve L, Grundmann, Patrick, Mavingui, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], KZ was supported by PhD fellowships from the French Ministère de l’Education Nationale, de la Recherche et des Nouvelles Technologies. We thank Martina Kyselková and Juliana Almario for advices on microarray experiments. We thank Laurence Mousson and Camilo Arias-Goeta for mosquito’s infection. This work was funded by grants from Agence National de la Recherche (ANR-06-SEST07 ChikVendoM) and Fondation pour la Recherche sur la Biodiversité (FRB, formely IFB, CD-AOOI-07-012) and was carried out within the frameworks of GDRI ‘Biodiversité et Développement Durable à Madagascar’ and COST action F0701 ‘Arthropod Symbioses: from fundamental to pest disease management’., ANR-06-SEST-0007,CHIKVENDOM,Interactions multipartites entre le virus chikungunya, les endosymbiostes et les moustiques. Impact sur la transmission virale et la dynamique des populations vectorielles ( 2006 ), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, ANR-06-SEST-0007,CHIKVENDOM,Interactions multipartites entre le virus chikungunya, les endosymbiostes et les moustiques. Impact sur la transmission virale et la dynamique des populations vectorielles(2006), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)
Subjects: chikungunya, OUTBREAK, MESH: Alphaproteobacteria, viruses, MESH : Symbiosis, Virus Replication, bacterial community, Aedes, MESH : Insect Vectors, INFECTION, MESH : Biodiversity, WOLBACHIA, MESH: Animals, MESH : Host-Pathogen Interactions, MESH: Symbiosis, virus diseases, Biodiversity, MESH: Aedes, MESH : Enterobacteriaceae, Aedes albopictus, MESH : Alphaproteobacteria, MESH : Bacterial Physiological Phenomena, MESH : Virus Replication, REUNION ISLAND, MESH: Gammaproteobacteria, MESH : Wolbachia, Host-Pathogen Interactions, TAXONOMIC MICROARRAY, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, POPULATIONS, MESH: Wolbachia, Chikungunya virus, microarray, Gammaproteobacteria, MESH : Bacteroidetes, DENGUE, TRANSMISSION, MESH: Insect Vectors, MESH : Chikungunya virus, Bacterial Physiological Phenomena, [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Biodiversity, AEGYPTI, MESH: Enterobacteriaceae, Enterobacteriaceae, MESH : Gammaproteobacteria, parasitic diseases, Animals, Symbiosis, Alphaproteobacteria, MESH : Aedes, Bacteroidetes, MESH: Virus Replication, MESH: Host-Pathogen Interactions, fungi, MESH: Chikungunya virus, AEDES-ALBOPICTUS, MESH: Bacteroidetes, Insect Vectors, MESH: Bacterial Physiological Phenomena, quantitative PCR, MESH : Animals
Abstract: International audience; Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However, there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha-and Gammaproteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection, whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. Our results clearly link the pathogen propagation with changes in the dynamics of the bacterial community, suggesting that cooperation or competition occurs within the host, which may in turn affect the mosquito traits like vector competence.
Published: 2012
Full Text: View/download PDF

14. Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (Diptera, Culicidae), in Madagascar

Author: Fara Nantenaina Raharimalala, Karima Zouache, Pierre Ravelonandro, Anna-Bella Failloux, Lala Sahondra Rafarasoa, Lala Harivelo Ravaomanarivo, Bakoly Olga Ralisoa, Claire Valiente Moro, Van Tran-Van, Patrick Mavingui, Eléonore Hellard, Laurence Mousson, Département d'Entomologie, Facultés des Sciences d'Antananarivo, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Centre National de Recherche sur l'Environnement, Centre National de recherche sur l'environnement, Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], Laboratoire de Biométrie et Biologie Evolutive ( LBBE ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique ( Inria ) -Centre National de la Recherche Scientifique ( CNRS ), This work was funded by the grant FRB-CD-AOOI-07- 012, and was carried out within the frameworks of GDRI 'Biodiversité et Développement Durable à Madagascar' and COST action F0701 'Arthropod Symbioses: from fundamental to pest disease management'., Université d'Antananarivo, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Ecoépidémiologie évolutionniste, Département écologie évolutive [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Centre National de Recherche sur l'Environnement (CNRE), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
Subjects: Male, Aedes albopictus, viruses, Molecular Sequence Data, 030231 tropical medicine, Zoology, Aedes aegypti, medicine.disease_cause, DNA, Mitochondrial, Arbovirus, Dengue fever, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Aedes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Madagascar, medicine, Animals, lcsh:RC109-216, Chikungunya, 030304 developmental biology, 0303 health sciences, biology, Ecology, Research, fungi, Outbreak, virus diseases, NADH Dehydrogenase, Sequence Analysis, DNA, medicine.disease, biology.organism_classification, 3. Good health, Phylogeography, [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Infectious Diseases, Vector (epidemiology), Cyclooxygenase 1, Female, Parasitology, SDV:MHEP:MI, Chikungunya virus, Entomology
Abstract: Background In the past ten years, the Indian Ocean region has been the theatre of severe epidemics of chikungunya and dengue. These outbreaks coincided with a high increase in populations of Aedes albopictus that outcompete its sister taxon Aedes aegypti in most islands sampled. The objective of this work was to update the entomological survey of the two Aedes species in the island of Madagascar which has to face these arboviroses. Methods The sampling of Aedes mosquitoes was conducted during two years, from October 2007 to October 2009, in fifteen localities from eight regions of contrasting climates. Captured adults were identified immediately whereas immature stages were bred until adult stage for determination. Phylogenetic analysis was performed using two mtDNA genes, COI and ND5 and trees were constructed by the maximum likelihood (ML) method with the gene time reversible (GTR) model. Experimental infections with the chikungunya virus strain 06.21 at a titer of 107.5 pfu/mL were performed to evaluate the vector competence of field-collected mosquitoes. Disseminated infection rates were measured fourteen days after infection by immunofluorescence assay performed on head squashes. Results The species Aedes aegypti was detected in only six sites in native forests and natural reserves. In contrast, the species Aedes albopictus was found in 13 out of the 15 sites sampled. Breeding sites were mostly found in man-made environments such as discarded containers, used tires, abandoned buckets, coconuts, and bamboo cuts. Linear regression models showed that the abundance of Ae. albopictus was significantly influenced by the sampling region (F = 62.00, p < 2.2 × 10-16) and period (F = 36.22, p = 2.548 × 10-13), that are associated with ecological and climate variations. Phylogenetic analysis of the invasive Ae. albopictus distinguished haplotypes from South Asia and South America from those of Madagascar, but the markers used were not discriminant enough to discern Malagasy populations. The experimental oral infection method showed that six Ae. albopictus populations exhibited high dissemination infection rates for chikungunya virus ranging from 98 to 100%. Conclusion In Madagascar, Ae. albopictus has extended its geographical distribution whereas, Ae. aegypti has become rare, contrasting with what was previously observed. Changes are predominantly driven by human activities and the rainfall regime that provide suitable breeding sites for the highly anthropophilic mosquito Ae. albopictus. Moreover, these populations were found to be highly susceptible to chikungunya virus. In the light of this study, Ae. albopictus may have been involved in the recent outbreaks of chikungunya and dengue epidemics in Madagascar, and consequently, control measures should be promoted to limit its current expansion.
Published: 2012
Full Text: View/download PDF

15. Detection of dengue group viruses by fluorescence in situ hybridization

Author: Catherine Legras-Lachuer, Vincent Raquin, Martin Wannagat, Karima Zouache, Patrick Mavingui, Claire Valiente Moro, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Pôle Rhône-Alpin de BioInformatique [Lyon] (PRABI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], ANR-2010-BLAN-170101 (ImmunSymbArt), ACIP A-10-2009 and FRBCD- AOOI-07-12, and was carried out within the framework of the GDRIs 'Biodiversity and Infectious Diseases' and 'Biodiversité et Développement durable', Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Arbovirus et Insectes Vecteurs, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Pôle Rhône-Alpes de Bioinformatique ( PRABI ), and Pôle Rhône-Alpes de Bioinformatique
Subjects: Serotype, Aedes albopictus, viruses, 030231 tropical medicine, Fluorescence In Situ Hybridization, Dengue virus, medicine.disease_cause, Sensitivity and Specificity, Salivary Glands, Virus, Cell Line, lcsh:Infectious and parasitic diseases, Dengue fever, Dengue, 03 medical and health sciences, 0302 clinical medicine, Aedes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Virology, medicine, Animals, lcsh:RC109-216, In Situ Hybridization, Fluorescence, 030304 developmental biology, 0303 health sciences, biology, medicine.diagnostic_test, Research, Yellow fever, virus diseases, Dengue Virus, biochemical phenomena, metabolism, and nutrition, medicine.disease, biology.organism_classification, 3. Good health, Flavivirus, [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Infectious Diseases, Parasitology, Oligonucleotide Probes, SDV:MHEP:MI, Fluorescence in situ hybridization
Abstract: Background Dengue fever (DF) and dengue hemorrhagic fever (DHF) represent a global challenge in public health. It is estimated that 50 to 100 million infections occur each year causing approximately 20,000 deaths that are usually linked to severe cases like DHF and dengue shock syndrome. The causative agent of DF is dengue virus (genus Flavivirus) that comprises four distinct serotypes (DENV-1 to DENV-4). Fluorescence in situ hybridization (FISH) has been used successfully to detect pathogenic agents, but has not been implemented in detecting DENV. To improve our understanding of DENV infection and dissemination in host tissues, we designed specific probes to detect DENV in FISH assays. Methods Oligonucleotide probes were designed to hybridize with RNA from the broadest range of DENV isolates belonging to the four serotypes, but not to the closest Flavivirus genomes. Three probes that fit the criteria defined for FISH experiments were selected, targeting both coding and non-coding regions of the DENV genome. These probes were tested in FISH assays against the dengue vector Aedes albopictus (Diptera: Culicidae). The FISH experiments were led in vitro using the C6/36 cell line, and in vivo against dissected salivary glands, with epifluorescence and confocal microscopy. Results The three 60-nt oligonucleotides probes DENV-Probe A, B and C cover a broad range of DENV isolates from the four serotypes. When the three probes were used together, specific fluorescent signals were observed in C6/36 infected with each DENV serotypes. No signal was detected in either cells infected with close Flavivirus members West Nile virus or yellow fever virus. The same protocol was used on salivary glands of Ae. albopictus fed with a DENV-2 infectious blood-meal which showed positive signals in the lateral lobes of infected samples, with no significant signal in uninfected mosquitoes. Conclusion Based on the FISH technique, we propose a way to design and use oligonucleotide probes to detect arboviruses. Results showed that this method was successfully implemented to specifically detect DENV in a mosquito cell line, as well as in mosquito salivary glands for the DENV-2 serotype. In addition, we emphasize that FISH could be an alternative method to detect arboviruses in host tissues, also offering to circumvent the discontinuity of antibodies used in immunofluorescent assays.
Published: 2012
Full Text: View/download PDF

16. Bidirectional Interactions between Arboviruses and the Bacterial and Viral Microbiota in Aedes aegypti and Culex quinquefasciatus

Author: Chenyan Shi, Leen Beller, Lanjiao Wang, Ana Rosales Rosas, Lander De Coninck, Lyza Héry, Laurence Mousson, Nonito Pagès, Jeroen Raes, Leen Delang, Anubis Vega-Rúa, Anna-bella Failloux, Jelle Matthijnssens, Shenzhen University [Shenzhen], Rega Institute for Medical Research [Leuven, België], Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), VIB-KU Leuven Center for Microbiology [Leuven, Belgium], This work was supported by the fellowship of China Postdoctoral Science Foundation (2021M691238), by the French Government's Investissement d'Avenir program, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant no. ANR-10-LABX-62-IBEID), by a KU Leuven C2 grant to L.D. (C22/18/007), and by the Program Opérationnel FEDER-Guadeloupe-Conseil Régional 2014–2020 (grant 2018-FED-1084)., and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Subjects: Sucrose, virome, Bacteria, Zika Virus Infection, Microbiota, [SDV]Life Sciences [q-bio], Water, microbiome, Zika Virus, Mosquito Vectors, Microbiology, Culex, arbovirus, Aedes, phageome, Virology, Viruses, single mosquito metagenomics, Humans, Animals, Female, mosquito-arbovirus-microbiota interaction, West Nile virus, Arboviruses
Abstract: Mosquitoes are important vectors for many arboviruses. It is becoming increasingly clear that various symbiotic microorganisms (including bacteria and insect-specific viruses; ISVs) in mosquitoes have the potential to modulate the ability of mosquitoes to transmit arboviruses. In this study, we compared the bacteriome and virome (both eukaryotic viruses and bacteriophages) of female adult Aedes aegypti and Culex quinquefasciatus mosquitoes fed with sucrose/water, blood, or blood spiked with Zika virus (ZIKV) or West Nile virus (WNV), respectively. Furthermore, we investigated associations between the microbiota and vector competence. We show that the influence of arboviruses on the mosquito microbiome-and vice versa-is distinct for each combination of arbovirus/mosquito species. The presence of ZIKV resulted in a temporarily increased Aedes ISV diversity. However, this effect was distinct for different ISVs: some ISVs decreased following the blood meal (Aedes aegypti totivirus), whereas other ISVs increased only when the blood contained ZIKV (Guadeloupe mosquito virus). Also, the diversity of the Aedes bacteriome depended on the diet and the presence of ZIKV, with a lower diversity observed for mosquitoes receiving blood without ZIKV. In Cx. quinquefasciatus, some ISVs increased in WNV-infected mosquitoes (Guadeloupe Culex tymo-like virus). Particularly, the presence of Wenzhou sobemo-like virus 3 (WSLV3) was associated with the absence of infectious WNV in mosquito heads, suggesting that WSLV3 might affect vector competence for WNV. Distinct profiles of bacteriophages were identified in Culex mosquitoes depending on diet, despite the lack of clear changes in the bacteriome. Overall, our data demonstrate a complex three-way interaction among arboviruses, resident microbiota, and the host, which is distinct for different arbovirus-mosquito combinations. A better understanding of these interactions may lead to the identification of microbiota able to suppress the ability of arbovirus transmission to humans, and hence improved arbovirus control measures. IMPORTANCE In this study, we first utilized the single mosquito microbiome analysis, demonstrating a complex three-way interaction among arboviruses, resident microbiota, and the host, which is distinct for different arbovirus-mosquito combinations. Some of the previously described "core virus" increased in the mosquitos receiving viral blood meal, like Guadeloupe mosquito virus and Guadeloupe Culex tymo-like virus, suggesting their potential roles in ZIKV and WNV infection. Notably, Wenzhou sobemo-like virus 3 was associated with the absence of infectious WNV in heads of Culex mosquitoes, which might affect vector competence for WNV. A better understanding of these interactions will lead to the identification of microbiota able to suppress the ability of arbovirus transmission to humans, and hence improved arbovirus control measures. ispartof: MBIO vol:13 issue:5 ispartof: location:United States status: published
Published: 2022
Full Text: View/download PDF

17. Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project

Author: Thomas Obadia, Gladys Gutierrez-Bugallo, Veasna Duong, Ana I. Nuñez, Rosilainy S. Fernandes, Basile Kamgang, Liza Hery, Yann Gomard, Sandra R. Abbo, Davy Jiolle, Uros Glavinic, Myrielle Dupont-Rouzeyrol, Célestine M. Atyame, Nicolas Pocquet, Sébastien Boyer, Catherine Dauga, Marie Vazeille, André Yébakima, Michael T. White, Constantianus J. M. Koenraadt, Patrick Mavingui, Anubis Vega-Rua, Eva Veronesi, Gorben P. Pijlman, Christophe Paupy, Núria Busquets, Ricardo Lourenço-de-Oliveira, Xavier De Lamballerie, Anna-Bella Failloux, Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Epidémiologie et Analyse des Maladies Infectieuses - Infectious Disease Epidemiology and Analytics, Pedro Kouri Institute of Tropical Medicine, Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur du Cambodge, Centre de Recerca en Sanitat Animal [UAB, Spain] (CReSA), Universitat Autònoma de Barcelona (UAB)-Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA), Instituto Oswaldo Cruz / Oswaldo Cruz Institute [Rio de Janeiro] (IOC), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre for Research in Infectious Diseases [Yaoundé] (CRID), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), Universität Zürich [Zürich] = University of Zurich (UZH), Institut Pasteur de Nouvelle-Calédonie, Entomologie médicale [Nouméa, Nouvelle-Calédonie] (URE-EM), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, VECCOTRA, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Tis study was supported by the European Union’s Horizon 2020 research and innovation program under ZIKAlliance grant agreement no. 734548., European Project: 734548,ZIKAlliance(2016), Fundação Oswaldo Cruz (FIOCRUZ), Obadia, Thomas, A global alliance for Zika virus control and prevention - ZIKAlliance - 2016-10-01 - 2019-09-30 - 734548 - VALID, University of Zurich, Failloux, Anna-Bella, Producció Animal, and Sanitat Animal
Subjects: 10078 Institute of Parasitology, Laboratory of Virology, General Physics and Astronomy, 610 Medicine & health, 1600 General Chemistry, Genetics and Molecular Biology, MESH: Zika Virus, Mosquito Vectors, Virus-host interactions, General Biochemistry, Genetics and Molecular Biology, Disease Outbreaks, Laboratorium voor Virologie, MESH: Zika Virus Infection, 1300 General Biochemistry, Genetics and Molecular Biology, Aedes, 600 Technology, Life Science, Animals, Humans, MESH: Animals, Laboratory of Entomology, MESH: Disease Outbreaks, 1000 Multidisciplinary, MESH: Humans, Multidisciplinary, Zika Virus Infection, Virus–host interactions, MESH: Infant, Newborn, Infant, Newborn, Zika Virus, MESH: Aedes, General Chemistry, PE&RC, Laboratorium voor Entomologie, 3100 General Physics and Astronomy, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, General Biochemistry, 570 Life sciences, biology, MESH: Mosquito Vectors, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Entomology
Abstract: First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus, both colonizing a large strip encompassing tropical and temperate regions. As part of the international project ZIKAlliance initiated in 2016, 50 mosquito populations from six species collected in 12 countries were experimentally infected with different Zika viruses. Here, we show that Ae. aegypti is mainly responsible for Zika virus transmission having the highest susceptibility to viral infections. Other species play a secondary role in transmission while Culex mosquitoes are largely non-susceptible. Zika strain is expected to significantly modulate transmission efficiency with African strains being more likely to cause an outbreak. As the distribution of Ae. aegypti will doubtless expand with climate change and without new marketed vaccines, all the ingredients are in place to relive a new pandemic of Zika. Zika virus (ZIKV), the causative agent of virus-induced brain damage in newborns, is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus. Here, Obadia et al. characterize ZIKV vector competence of 50 mosquito populations from six species collected in 12 different countries to inform about epidemic risk. They find that African ZIKV strain shows higher transmission efficiency compared to American and Asian ZIKV strains and that Ae. aegypti mosquitoes have highest susceptibility to infections, while Culex mosquitoes are largely non-susceptible.
Published: 2022
Full Text: View/download PDF

18. Infravec2 guidelines for the design and operation of containment level 2 and 3 insectaries in Europe

Author: Pondeville, Emilie, Failloux, Anna-Bella, Simard, Frederic, Volf, Petr, Crisanti, Andrea, Haghighat-Khah, Roya Elaine, Busquets, Núria, Abad, Francesc Xavier, Wilson, Anthony J, Bellini, Romeo, Marsh Arnaud, Sarah, Kohl, Alain, Veronesi, Eva, Producció Animal, Sanitat Animal, MRC - University of Glasgow Centre for Virus Research, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Charles University [Prague] (CU), Imperial College London, Centre de Recerca en Sanitat Animal [UAB, Spain] (CReSA), Universitat Autònoma de Barcelona (UAB)-Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA), The Pirbright Institute, Biotechnology and Biological Sciences Research Council (BBSRC), Centro Agricoltura Ambiente 'Giorgio Nicoli' (CAA), Génétique et Génomique des Insectes Vecteurs - Genetics and Genomics of Insect Vectors, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Universität Zürich [Zürich] = University of Zurich (UZH), This work was supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 731060, the UK Medical Research Council to AK and EP under grant Medical Research Council (MRC) MC_UU_12014/8, the ANR to ABF under grant n° ANR-10-LABX-62-IBEID, the CERCA Programme., Generalitat de Catalunya to NB and FXA, the ERD funds CZ.02.1.01/0.0/0.0/16_019/0000759 to PV under grant CZ.02.1.01/0.0/0.0/16_019/000075., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 731060,INFRAVEC2(2017), University of Zurich, Pondeville, Emilie, and Veronesi, Eva
Subjects: 10078 Institute of Parasitology, containment levels, vector–pathogen interactions, [SDV]Life Sciences [q-bio], 2404 Microbiology, Environmental and Occupational Health, 2405 Parasitology, Public Health, Environmental and Occupational Health, Vector biology, 610 Medicine & health, 2739 Public Health, Environmental and Occupational Health, 2725 Infectious Diseases, General Medicine, research infrastructures, Microbiology, Infectious Diseases, 600 Technology, 570 Life sciences, biology, Parasitology, Public Health, guidelines, insectaries
Abstract: With the current expansion of vector-based research and an increasing number of facilities rearing arthropod vectors and infecting them with pathogens, common measures for containment of arthropods as well as manipulation of pathogens are becoming essential for the design and running of such research facilities to ensure safe work and reproducibility, without compromising experimental feasibility. These guidelines and comments were written by experts of the Infravec2 consortium, a Horizon 2020-funded consortium integrating the most sophisticated European infrastructures for research on arthropod vectors of human and animal diseases. They reflect current good practice across European laboratories with experience of safely handling different mosquito species and the pathogens they transmit. As such, they provide experience-based advice to assess and manage the risks to work safely with mosquitoes and the pathogens they transmit. This document can also form the basis for research with other arthropods, for example, midges, ticks or sandflies, with some modification to reflect specific requirements. info:eu-repo/semantics/publishedVersion
Published: 2022
Full Text: View/download PDF

19. MicroRNAs and other small RNAs in Aedes aegypti saliva and salivary glands following chikungunya virus infection

Author: Carmine Fiorillo, Pei-Shi Yen, Alessio Colantoni, Marina Mariconti, Nayara Azevedo, Fabrizio Lombardo, Anna-Bella Failloux, Bruno Arcà, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), European Molecular Biology Laboratory [Heidelberg] (EMBL), This study was supported by Sapienza University of Rome (Finanziamenti di Ateneo, RM11916B75AABA65), the French Government's Investissement d'Avenir program, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID) and the European Union's Horizon 2020 research and innovation programme under grant agreement No 731060 (Infravec2, Research Infrastructures for the control of vector-borne diseases, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and European Project: 731060,INFRAVEC2(2017)
Subjects: Multidisciplinary, Animals, Chikungunya Fever, Humans, Mosquito Vectors, RNA, Small Interfering, Saliva, Salivary Glands, Aedes, Chikungunya virus, MicroRNAs, [SDV]Life Sciences [q-bio], Small Interfering, stomatognathic system, RNA
Abstract: Mosquito saliva facilitates blood feeding through the anti-haemostatic, anti-inflammatory and immunomodulatory properties of its proteins. However, the potential contribution of non-coding RNAs to host manipulation is still poorly understood. We analysed small RNAs from Aedes aegypti saliva and salivary glands and show here that chikungunya virus-infection triggers both the siRNA and piRNA antiviral pathways with limited effects on miRNA expression profiles. Saliva appears enriched in specific miRNA subsets and its miRNA content is well conserved among mosquitoes and ticks, clearly pointing to a non-random sorting and occurrence. Finally, we provide evidence that miRNAs from Ae. aegypti saliva may target human immune and inflammatory pathways, as indicated by prediction analysis and searching for experimentally validated targets of identical human miRNAs. Overall, we believe these observations convincingly support a scenario where both proteins and miRNAs from mosquito saliva are injected into vertebrates during blood feeding and contribute to the complex vector–host–pathogen interactions.
Published: 2022
Full Text: View/download PDF

20. Risk of yellow fever virus transmission in the Asia-Pacific region

Author: Thomas Obadia, Laurence Mousson, Basile Kamgang, Yoann Madec, Marie Vazeille, Chun-Hong Chen, Anna-Bella Failloux, Pei-Shi Yen, Lucy de Guilhem de Lataillade, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Malaria : parasites et hôtes - Malaria : parasites and hosts, Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Centre for Research in Infectious Diseases [Yaoundé] (CRID), National Health Research Institutes [Taiwan] (NHRI), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), and HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)
Subjects: 0301 basic medicine, Asia, Epidemiology, Virus transmission, Science, 030231 tropical medicine, Viral transmission, General Physics and Astronomy, Aedes aegypti, Subtropics, Asia pacific region, Article, General Biochemistry, Genetics and Molecular Biology, Virus, 03 medical and health sciences, 0302 clinical medicine, Aedes, Risk Factors, Yellow Fever, parasitic diseases, medicine, Animals, Saliva, lcsh:Science, Probability, Multidisciplinary, Geography, biology, Yellow fever, General Chemistry, Viral Load, biology.organism_classification, medicine.disease, Virology, Insect Vectors, 3. Good health, Experimental models of disease, 030104 developmental biology, Viral infection, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Linear Models, lcsh:Q, Yellow fever virus, Viral load
Abstract: Historically endemic to Sub-Saharan Africa and South America, yellow fever is absent from the Asia-Pacific region. Yellow fever virus (YFV) is mainly transmitted by the anthropophilic Aedes mosquitoes whose distribution encompasses a large belt of tropical and sub tropical regions. Increasing exchanges between Africa and Asia have caused imported YFV incidents in non-endemic areas, which are threatening Asia with a new viral emergence. Here, using experimental infections of field-collected mosquitoes, we show that Asian-Pacific Aedes mosquitoes are competent vectors for YFV. We observe that Aedes aegypti populations from Singapore, Taiwan, Thailand, and New Caledonia are capable of transmitting YFV 14 days after oral infections, with a number of viral particles excreted from saliva reaching up to 23,000 viral particles. These findings represent the most comprehensive assessment of vector competence and show that Ae. aegypti mosquitoes from the Asia-Pacific region are highly competent to YFV, corroborating that vector populations are seemingly not a brake to the emergence of yellow fever in the region., Yellow fever is absent from the Asia/Pacific region, despite presence of the mosquito vector. Here, the authors demonstrate that mosquitoes collected from field sites across the region are capable of transmitting yellow fever virus, indicating that vector competence is not a barrier to disease spread.
Published: 2020
Full Text: View/download PDF

21. Impact of temperature on dengue and chikungunya transmission by the mosquito Aedes albopictus

Author: Aurélien Mercier, Thomas Obadia, Davide Carraretto, Enkelejda Velo, Gaelle Gabiane, Silvia Bino, Marie Vazeille, Giuliano Gasperi, Catherine Dauga, Anna R. Malacrida, Paul Reiter, Anna-Bella Failloux, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Insectes et Maladies Infectieuses, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Pavia = University of Pavia (UNIPV), Institute of Public Health - Instituti i Shëndetit Publik [Tirana] (ISHP), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Epidémiologie et Analyse des Maladies Infectieuses - Infectious Disease Epidemiology and Analytics, This work was supported by the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID and the European Commission under the 7th Framework under DengueTools Grant Agreement no. 282589., We thank Charlotte Balière and Valérie Caro for technical assistance in mosquito genotyping. We also thank Perparim Kadriaj and Erjon Muhaxhiri for their contribution in collecting mosquito eggs., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and European Project: 282589,EC:FP7:HEALTH,FP7-HEALTH-2011-single-stage,DENGUETOOLS(2011)
Subjects: Dengue, Multidisciplinary, Aedes, Temperature, Animals, Chikungunya Fever, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Chikungunya virus
Abstract: The mosquito Aedes albopictus is an invasive species first detected in Europe in Albania in 1979, and now established in 28 European countries. Temperature is a limiting factor in mosquito activities and in the transmission of associated arboviruses namely chikungunya (CHIKV) and dengue (DENV). Since 2007, local transmissions of CHIKV and DENV have been reported in mainland Europe, mainly in South Europe. Thus, the critical question is how far north transmission could occur. In this context, the Albanian infestation by Ae. albopictus is of interest because the species is present up to 1200 m of altitude; this allows using altitude as a proxy for latitude. Here we show that Ae. albopictus can transmit CHIKV at 28 °C as well as 20 °C, however, the transmission of DENV is only observed at 28 °C. We conclude that if temperature is the key environmental factor limiting transmission, then transmission of CHIKV, but not DENV is feasible in much of Europe.
Published: 2022
Full Text: View/download PDF

22. West Nile, Sindbis and Usutu Viruses: Evidence of Circulation in Mosquitoes and Horses in Tunisia

Author: Youmna M’ghirbi, Laurence Mousson, Sara Moutailler, Sylvie Lecollinet, Rayane Amaral, Cécile Beck, Hajer Aounallah, Meriem Amara, Ahmed Chabchoub, Adel Rhim, Anna-Bella Failloux, Ali Bouattour, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de la Manouba [Tunisie] (UMA), and This study was funded by Pasteur Institute of Tunis, Tunisia and Pasteur Institute of Paris, France in the frame of MATI project-ENVIRONMOS, and the laboratory of Viruses, Vectors and Hosts (LR20IPT02) funded by the Ministry of Higher Education and Scientific Research of Tunisia.
Subjects: Microbiology (medical), microsphere immunoassay, [SDV]Life Sciences [q-bio], Virus de sindbis, L73 - Maladies des animaux, Immunology and Allergy, Surveillance épidémiologique, Molecular Biology, Transmission des maladies, horses, mosquito-borne-viruses, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, General Immunology and Microbiology, virus du Nil occidental, high-throughput real-time PCR, Maladie transmise par vecteur, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, seroneutralization test, cELISA assay, Equus caballus, L72 - Organismes nuisibles des animaux, Cheval
Abstract: International audience; Mosquito-borne diseases have a significant impact on humans and animals and this impact is exacerbated by environmental changes. However, in Tunisia, surveillance of the West Nile virus (WNV) is based solely on the surveillance of human neuroinvasive infections and no study has reported mosquito-borne viruses (MBVs), nor has there been any thorough serological investigation of anti-MBV antibodies in horses. This study therefore sought to investigate the presence of MBVs in Tunisia. Among tested mosquito pools, infections by WNV, Usutu virus (USUV), and Sindbis virus (SINV) were identified in Cx. perexiguus. The serosurvey showed that 146 of 369 surveyed horses were positive for flavivirus antibodies using the cELISA test. The microsphere immunoassay (MIA) showed that 74 of 104 flavivirus cELISA-positive horses were positive for WNV, 8 were positive for USUV, 7 were positive for undetermined flaviviruses, and 2 were positive for tick-borne encephalitis virus (TBEV). Virus neutralization tests and MIA results correlated well. This study is the first to report the detection of WNV, USUV and SINV in Cx. perexiguus in Tunisia. Besides, it has shown that there is a significant circulation of WNV and USUV among horses, which is likely to cause future sporadic outbreaks. An integrated arbovirus surveillance system that includes entomological surveillance as an early alert system is of major epidemiological importance.
Published: 2023
Full Text: View/download PDF

23. Aedes mosquitoes in the emerging threat of urban yellow fever transmission

Author: Gaelle Gabiane, Pei‐Shi Yen, Anna‐Bella Failloux, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Université des Antilles (Pôle Martinique), Université des Antilles (UA), This work was supported by the European Union's Horizon 2020 research and innovation program under (grant number 731060) (Infravec2, Research Infrastructures for the control of vector-borne diseases, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 731060,INFRAVEC2(2017), and European Project: 734548,ZIKAlliance(2016)
Subjects: Zika Virus Infection, [SDV]Life Sciences [q-bio], COVID-19, risk assessment, Mosquito Vectors, Zika Virus, yellow fever, Infectious Diseases, Aedes, Virology, Animals, Chikungunya Fever, Humans, Yellow fever virus, Aedes mosquitoes
Abstract: International audience; This last decade has seen a resurgence of yellow fever (YF) in historical endemic regions and repeated attempts of YF introduction in YF-free countries such as the Asia-Pacific region and the Caribbean. Infected travellers are the main entry routes in these regions where competent mosquito vectors proliferate in appropriate environmental conditions. With the discovery of the 17D vaccine, it was thought that YF would be eradicated. Unfortunately, it was not the case and, contrary to dengue, chikungunya and Zika, factors that cotribute to YF transmission remain under investigation. Today, all the signals are red and it is very likely that YF will be the next pandemic in the YF-free regions where millions of people are immunologically naïve. Unlike COVID-19, YF is associated with a high case-fatality rate and a high number of deaths are expected. This review gives an overview of global YF situation, including the non-endemic Asia-Pacific region and the Caribbean where Aedes aegypti is abundantly distributed, and also proposes different hypotheses on why YF outbreaks have not yet occurred despite high records of travellers importing YF into these regions and what role Aedes mosquitoes play in the emergence of urban YF.
Published: 2022
Full Text: View/download PDF

24. Vector Specificity of Arbovirus Transmission

Author: Viglietta, Marine, Bellone, Rachel, Blisnick, Adrien Albert, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), This study was supported by the French National Research Agency (Projet-ANR-19-CE35-0004), the European Union’s Horizon 2020 Research and Innovation Program under grant agreement no. 731060 (Infravec2, research infrastructures for the control of vector-borne diseases, We thank Patricia Smiley and Richard Paul for proofreading our English, ANR-19-CE35-0004,ArboVEC,Barrières d'hôtes dans la spécificité des interactions entre moustiques vecteurs et arbovirus(2019), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 731060,INFRAVEC2(2017), and Institut Pasteur [Paris]
Subjects: arbovirus, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, viruses, parasitic diseases, host-pathogen interactions, mosquito, Review, Microbiology, QR1-502, tick, host–pathogen interactions, vectorial transmission
Abstract: International audience; More than 25% of human infectious diseases are vector-borne diseases (VBDs). These diseases, caused by pathogens shared between animals and humans, are a growing threat to global health with more than 2.5 million annual deaths. Mosquitoes and ticks are the main vectors of arboviruses including flaviviruses, which greatly affect humans. However, all tick or mosquito species are not able to transmit all viruses, suggesting important molecular mechanisms regulating viral infection, dissemination, and transmission by vectors. Despite the large distribution of arthropods (mosquitoes and ticks) and arboviruses, only a few pairings of arthropods (family, genus, and population) and viruses (family, genus, and genotype) successfully transmit. Here, we review the factors that might limit pathogen transmission: internal (vector genetics, immune responses, microbiome including insect-specific viruses, and coinfections) and external, either biotic (adult and larvae nutrition) or abiotic (temperature, chemicals, and altitude). This review will demonstrate the dynamic nature and complexity of virus-vector interactions to help in designing appropriate practices in surveillance and prevention to reduce VBD threats.
Published: 2021
Full Text: View/download PDF

25. Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus

Author: Wei-Liang Liu, Chia-Wei Hsu, Shih-Peng Chan, Pei-Shi Yen, Matthew P. Su, Jian-Chiuan Li, Hsing-Han Li, Lie Cheng, Cheng-Kang Tang, Shih-Hsun Ko, Huai-Kuang Tsai, Zing Tsung-Yeh Tsai, Omar S. Akbari, Anna-Bella Failloux, Chun-Hong Chen, National Health Research Institutes [Taiwan] (NHRI), National Taiwan University [Taiwan] (NTU), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Nagoya University, Academia Sinica, University of California [San Diego] (UC San Diego), University of California (UC), and This study also was supported by the National Health Research Institutes (NHRI), Taiwan (Grant No. NHRI-MR-110-GP-12) and Ministry of Health and Welfare (Grant No. MOHW103-TDU-B-212-000005) awarded to Chun-Hong Chen.
Subjects: Mosquito Control, [SDV]Life Sciences [q-bio], viruses, MESH: Cricetinae, Transposases, MESH: Dengue, Pathogenesis, MESH: Dengue Virus, Dengue, MESH: Cricetulus, Aedes, Cricetinae, MESH: RNA, Small Interfering, 2.2 Factors relating to the physical environment, MESH: Animals, Aetiology, MESH: Mosquito Control, Multidisciplinary, virus diseases, MESH: Aedes, Viral Load, Infectious Diseases, Medicine, MESH: Mosquito Vectors, MESH: Viral Load, Infection, Biotechnology, Science, Genetically Modified, Mosquito Vectors, Small Interfering, Serogroup, Article, Cell Line, MESH: Animals, Genetically Modified, Vaccine Related, Cricetulus, Rare Diseases, Biodefense, Genetics, Animals, Prevention, MESH: Serogroup, Fibroblasts, Dengue Virus, MESH: Cell Line, Vector-Borne Diseases, Emerging Infectious Diseases, Good Health and Well Being, MESH: Fibroblasts, RNAi, MESH: Transposases, RNA
Abstract: The areas where dengue virus (DENV) is endemic have expanded rapidly, driven in part by the global spread of Aedes species, which act as disease vectors. DENV replicates in the mosquito midgut and is disseminated to the mosquito’s salivary glands for amplification. Thus, blocking virus infection or replication in the tissues of the mosquito may be a viable strategy for reducing the incidence of DENV transmission to humans. Here we used the mariner Mos1 transposase to create an Aedes aegypti line that expresses virus-specific miRNA hairpins capable of blocking DENV replication. These microRNA are driven by the blood-meal-inducible carboxypeptidase A promoter or by the polyubiquitin promoter. The transgenic mosquitoes exhibited significantly lower infection rates and viral titers for most DENV serotypes 7 days after receiving an infectious blood meal. The treatment was also effective at day 14 post infection after a second blood meal had been administered. In viral transmission assay, we found there was significantly reduced transmission in these lines. These transgenic mosquitoes were effective in silencing most of the DENV genome; such an approach may be employed to control a dengue fever epidemic.
Published: 2021
Full Text: View/download PDF

26. Absence of transmission of vYF next generation Yellow Fever vaccine in mosquitoes

Author: Rachel Bellone, Laurence Mousson, Chloé Bohers, Nathalie Mantel, Anna-Bella Failloux, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Sanofi Pasteur [Marcy-l'Étoile, France], and The project has been supported by Sanofi.
Subjects: [SDV]Life Sciences [q-bio], Yellow Fever Vaccine, Public Health, Environmental and Occupational Health, Mosquito Vectors, Vaccines, Attenuated, Infectious Diseases, Aedes, Chlorocebus aethiops, Yellow Fever, Animals, Humans, Yellow fever virus, Vero Cells, Antigens, Viral
Abstract: One of the most effective vaccines against an arbovirus is the YFV-17D live-attenuated vaccine developed in 1937 against Yellow Fever (YF). This vaccine replicates poorly in mosquitoes and consequently, is not transmitted by vectors. Vaccine shortages, mainly due to constrained productions based on pathogen-free embryonated eggs, led Sanofi to move towards alternative methods based on a state-of-the-art process using continuous cell line cultures in bioreactor. vYF-247 is a next-generation live-attenuated vaccine candidate based on 17D adapted to grow in serum-free Vero cells. For the development of a new vaccine, WHO recommends to document infectivity and replication in mosquitoes. Here we infected Aedes aegypti and Aedes albopictus mosquitoes with vYF-247 vaccine compared first to the YF-17D-204 reference Sanofi vaccines (Stamaril and YF-VAX) and a clinical human isolate S-79, provided in a blood meal at a titer of 6.5 Log ffu/mL and secondly, to the clinical isolate only at an increased titer of 7.5 Log ffu/mL. At different days post-infection, virus replication, dissemination and transmission were evaluated by quantifying viral particles in mosquito abdomen, head and thorax or saliva, respectively. Although comparison of vYF-247 to reference vaccines could not be completed to yield significant results, we showed that vYF-247 was not transmitted by both Aedes species, either laboratory strains or field-collected populations, compared to clinical strain S-79 at the highest inoculation dose. Combined with the undetectable to low level viremia detected in vaccinees, transmission of the vYF-247 vaccine by mosquitoes is highly unlikely.
Published: 2022
Full Text: View/download PDF

27. Towards a better understanding of Rift Valley fever epidemiology in the south-west of the Indian Ocean

Author: Betty Zumbo, Anna-Bella Failloux, Eric Cardinale, Gaelle Nicolas, Matthieu Roger, Thiery Nirina Jean Jose Nipomichene, Thomas Balenghien, Véronique Chevalier, Nohal Elissa, Vincent Michel Rakotoharinome, Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien (CRVOI), Université de La Réunion (UR), Animal et gestion intégrée des risques (UPR AGIRs), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Unité d'Entomologie Médicale [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Direction de la santé animale et phytosanitaire, Ministère de l'Agriculture de l'Elevage et de la Pêche, Service de lutte anti-vectorielle, Agence de Santé Océan Indien, Institut Pasteur [Paris], Animal et gestion intégrée des risques (Cirad-Bios-UPR 22 AGIRs), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and Arbovirus et Insectes Vecteurs
Subjects: Veterinary medicine, Rift Valley Fever, Range (biology), animal diseases, viruses, Review, L73 - Maladies des animaux, 0302 clinical medicine, Prévention des maladies, Epidemiology, Rift Valley fever, Socioeconomics, 0303 health sciences, biology, Goats, Contrôle de maladies, Fièvre de la Vallée du Rift, virus diseases, 3. Good health, Épidémiologie, Vecteur de maladie, Bunyaviridae, L72 - Organismes nuisibles des animaux, Genre humain, medicine.medical_specialty, Ruminant, Gestion du risque, 030231 tropical medicine, Cattle Diseases, Sheep Diseases, Relation hôte pathogène, Virus de la fièvre de la vallée du Rift, Arbovirus, 03 medical and health sciences, Indian Ocean Islands, parasitic diseases, medicine, Animals, Surveillance épidémiologique, 030304 developmental biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Goat Diseases, Sheep, General Veterinary, Outbreak, biology.organism_classification, medicine.disease, Rift Valley fever virus, veterinary(all), Indian ocean, Culicidae, Phlebovirus, Cattle
Abstract: Rift Valley fever virus (Phlebovirus, Bunyaviridae) is an arbovirus causing intermittent epizootics and sporadic epidemics primarily in East Africa. Infection causes severe and often fatal illness in young sheep, goats and cattle. Domestic animals and humans can be contaminated by close contact with infectious tissues or through mosquito infectious bites. Rift Valley fever virus was historically restricted to sub-Saharan countries. The probability of Rift Valley fever emerging in virgin areas is likely to be increasing. Its geographical range has extended over the past years. As a recent example, autochthonous cases of Rift Valley fever were recorded in 2007–2008 in Mayotte in the Indian Ocean. It has been proposed that a single infected animal that enters a naive country is sufficient to initiate a major outbreak before Rift Valley fever virus would ever be detected. Unless vaccines are available and widely used to limit its expansion, Rift Valley fever will continue to be a critical issue for human and animal health in the region of the Indian Ocean.
Published: 2013
Full Text: View/download PDF

28. Zika Virus Circulation in Mali

Author: Abdoulaye Dabo, Simon Cauchemez, Souleymane Sacko, Bourema Kouriba, Abdoulaye Djimde, Jan Felix Drexler, Anna-Bella Failloux, Pierre Gallian, Abdoul Karim Sangaré, Ogobara K. Doumbo, Issa Diarra, Drissa Coulibaly, Amatigue Zeguime, Xavier de Lamballerie, Nathanaël Hozé, Elif Nurtop, Bakary Fofana, Boris Pastorino, Stéphane Priet, Issaka Sagara, Mahamadou Ali Thera, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Malaria Research and Training Center [Bamako, Mali], Université de Bamako, Ministère de la Santé et des Affaires Sociales du Mali [Bamako, Mali], Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Etablissement Français du Sang - Alpes-Méditerranée (EFS - Alpes-Méditerranée), Etablissement Français du Sang, German Center for Infection Research, Partnersite Munich (DZIF), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Modélisation mathématique des maladies infectieuses - Mathematical modelling of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016), BUISINE, Soline, Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université des sciences, des techniques et des technologies de Bamako (USTTB), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Subjects: Microbiology (medical), Male, Epidemiology, 030231 tropical medicine, Population, vector-borne infections, lcsh:Medicine, Blood Donors, Zika Virus Circulation in Mali, Mali, Asymptomatic, Arbovirus, lcsh:Infectious and parasitic diseases, Zika virus, Serology, 03 medical and health sciences, 0302 clinical medicine, Seroepidemiologic Studies, medicine, Seroprevalence, Humans, lcsh:RC109-216, viruses, 030212 general & internal medicine, education, mosquitoes, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, education.field_of_study, biology, seroprevalence, Transmission (medicine), Zika Virus Infection, Research, lcsh:R, Outbreak, Zika Virus, biology.organism_classification, medicine.disease, Virology, 3. Good health, Infectious Diseases, arbovirus, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, medicine.symptom
Abstract: International audience; The circulation of Zika virus (ZIKV) in Mali has not been clearly characterized. Therefore, we conducted a serologic survey of 793 asymptomatic volunteers >15 years of age (2016), and 637 blood donors (2013) to assess the seroprevalence of ZIKV infection in 2 ecoclimatic regions of Mali, tropical savannah and warm semiarid region, using ELISA and seroneutralization assays. The overall seroprevalence was ≈12% and increased with age, with no statistical difference between male and female participants. In the warm semiarid study sites we detected immunological markers of an outbreak that occurred in the late 1990s in 18% (95% CI 13%-23%) of participants. In tropical savannah sites, we estimated a low rate of endemic transmission, with 2.5% (95% CI 2.0%-3.1%) of population infected by ZIKV annually. These data demonstrate the circulation of ZIKV in Mali and provide evidence of a previously unidentified outbreak that occurred in the late 1990s.
Published: 2020
Full Text: View/download PDF

29. European Aedes caspius mosquitoes are experimentally unable to transmit Zika virus

Author: Núria Pujol, Lotty Birnberg, Carles Aranda, Raquel Rivas, Ana I. Núñez, Anna-Bella Failloux, M. Verdún, Sandra Talavera, Núria Busquets, Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA), CReSA, Universitat Autònoma de Barcelona (UAB), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), This study was funded by CERCA Programme/Generalitat de Catalunya. and the European Union’s Horizon 2020 Research and Innovation Programme under ZIKAlliance Grant Agreement no. 734548., The authors are very grateful to BSL3 facilities staff for their excellent technical contribution and to Dr. Francesc Xavier Abad for his excellent BSL3 facilities management at CReSA (IRTA)., European Project: 734548,ZIKAlliance(2016), Producció Animal, Sanitat Animal, Institute of Agrifood Research and Technology (IRTA), and Institut Pasteur [Paris]
Subjects: Entomology, 030231 tropical medicine, Population, Short Report, Mosquito Vectors, Biology, Arbovirus, Virus, Zika virus, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Aedes, Zoonosi, Aedes caspius, medicine, Animals, Transmission, lcsh:RC109-216, education, Saliva, 030304 developmental biology, 0303 health sciences, education.field_of_study, Zika Virus Infection, Zika Virus, Viral Load, Malalties infeccioses, medicine.disease, biology.organism_classification, Virology, 3. Good health, Europe, Infectious Diseases, Parasitology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, Viral load, Vector competence
Abstract: This study was funded by CERCA Programme/Generalitat de Catalunya. and the European Union's Horizon 2020 Research and Innovation Programme under ZIKAlliance Grant Agreement no. 734548. Background: Aedes caspius (Pallas, 1771) is a floodwater mosquito species widely distributed in the Western Palaearctic. As an anthropophilic species, its role as an arbovirus vector may be the key for understanding the transmission cycle of certain diseases in Europe such as Zika virus (ZIKV). Concerning vector competence for ZIKV, studies related to Ae. caspius are still scarce. ZIKV is an arbovirus that has provoked a widespread epidemic in the Pacific region (2007-2013) and in the Americas (2015-2016). ZIKV is associated with serious neurological injuries (e.g. microcephaly) and Guillain-Barré syndrome. Due to the ZIKV epidemics in the American continent, some viraemic travellers coming from endemic countries have been reported in Europe. More knowledge is therefore required to define the susceptibility of autochthonous mosquito species such as Ae. caspius for ZIKV in order to improve arbovirus surveillance and control programmes. In the present study, the vector competence of a European population of Ae. caspius was evaluated for two ZIKV lineages, the Suriname ZIKV strain (Asian lineage) and the MR766 ZIKV strain (African I lineage). Females were tested at 7, 14 and 21 days post-exposure (dpe) to infectious blood meals. An Ae. aegypti PAEA strain was used as a positive control. Results: Aedes caspius presented low susceptibility to ZIKV infection and the virus was only detected by RT-qPCR in body samples. Low viral loads were detected for the MR766 strain at 7 dpe and for the Suriname strain at 14 and 21 dpe. Aedes caspius was unable to produce a disseminated infection and virus transmission at any of the tested time points. Using Ae. aegypti PAEA strain, infection, dissemination and transmission rates were calculated for the Suriname ZIKV strain (Asian lineage) at each time point. For the MR766 ZIKV strain (African I lineage), while only infection rates were estimated at each time point, no dissemination or transmission were detected in either species. Conclusions: The results of the present study reveal that the tested Ae. caspius population has a strong midgut escape barrier that limits the dissemination or transmission of the virus. As such, it seems unlikely that European Ae. caspius mosquitoes could be involved in ZIKV transmission if ZIKV was introduced into Europe. This information may help in designing a better strategy to European surveillance and control programmes for ZIKV.
Published: 2019
Full Text: View/download PDF

30. Author Correction: Improved reference genome of the arboviral vector Aedes albopictus

Author: Pascal Miesen, Rebecca Halbach, Omar S. Akbari, Philippos Aris Papathanos, Elisa Pischedda, Ronald P. van Rij, Flavia Krsticevic, Françoise Thibaud-Nissen, Atashi Sharma, Arang Rhie, Luciano V. Cosme, Michele Marconcini, Zhijian Tu, Anna-Bella Failloux, Jeffrey R. Powell, Maria V. Sharakhova, James K. Biedler, Jacob E. Crawford, Reem A. Masri, Sergey Koren, D. A. Karagodin, Igor Antoshechkin, Jeremy Jenrette, Stephanie Gamez, Adam M. Phillippy, Umberto Palatini, Adalgisa Caccone, Mariangela Bonizzoni, J. Spencer Johnston, Patrick Masterson, Jay Ghurye, Università degli Studi di Pavia, Virginia Polytechnic Institute and State University [Blacksburg], Yale University [New Haven], National Human Genome Research Institute (NHGRI), National Center for Biotechnology Information (NCBI), The Hebrew University of Jerusalem (HUJ), Texas A&M University [College Station], Radboud Institute for Molecular Life Sciences [Nijmegen, the Netherlands], Verily Life Sciences Inc, California Institute of Technology (CALTECH), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Siberian Branch of the Russian Academy of Sciences (SB RAS), University of California [San Diego] (UC San Diego), University of California, Tomsk State University [Tomsk], Università degli Studi di Pavia = University of Pavia (UNIPV), Institut Pasteur [Paris] (IP), and University of California (UC)
Subjects: Aedes albopictus, QH301-705.5, [SDV]Life Sciences [q-bio], Computational biology, Mosquito Vectors, Biology, QH426-470, Chromosomes, 03 medical and health sciences, 0302 clinical medicine, Genome Size, Aedes, Genetics, Animals, RNA, Small Interfering, Biology (General), Author Correction, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Genome, Immunity, Chromosome Mapping, biology.organism_classification, Human genetics, 3. Good health, Insect Vectors, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Vector (epidemiology), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Transcriptome, 030217 neurology & neurosurgery, Arboviruses, Reference genome
Abstract: The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes.We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes.The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.
Published: 2021
Full Text: View/download PDF

31. Experimental infections with Zika virus strains reveal high vector competence of Aedes albopictus and Aedes aegypti populations from Gabon (Central Africa) for the African virus lineage

Author: Jiolle, Davy, Moltini-Conclois, Isabelle, Obame-Nkoghe, Judicaël, Yangari, Patrick, Porciani, Angélique, Scheid, Bethsabée, Kengne, Pierre, Ayala, Diego, Failloux, Anna-Bella, Paupy, Christophe, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre International de Recherches Médicales de Franceville (CIRMF), Université des Sciences et Techniques de Masuku [Franceville, Gabon] (USTM), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], This work was supported by H2020 Research Infrastructures [grant number Infravec2, no. 731060], Horizon 2020 Framework Programme [grant number ZIKAlliance, no.734548], and Agence Nationale de la Recherche [grant number TIGERBRIDGE, no. ANR-16-CE35-0010-01]., The authors thank Marie Vazeille for standardizing the experimental protocols, Catherine Dauga for her help in viral sequence alignments and the EVAg© initiative for providing the viral strains., ANR-16-CE35-0010,TIGERBRIDGE,Invasion d'Ae. albopictus dans les écosystèmes forestiers d'Afrique Centrale et risque d'émergence de nouveaux virus zoonotiques(2016), European Project: 731060,INFRAVEC2(2017), European Project: 734548,ZIKAlliance(2016), Université des Sciences et Techniques de Masuku (USTM), and Institut Pasteur [Paris] (IP)
Subjects: Asia, Zika Virus Infection, [SDV]Life Sciences [q-bio], fungi, Mosquito Vectors, Viral Load, Aedes albopictus, Zika virus, Aedes aegypti, Aedes, parasitic diseases, African and Asian lineages, Animals, Gabon, Saliva, Africa South of the Sahara, Research Article
Abstract: International audience; The two main Zika virus (ZIKV) vectors, Aedes albopictus and Aedes aegypti (invasive and native species, respectively), are present in Gabon (Central Africa). The aim of this study was to determine the entomological ZIKV risk associated with these mosquito species in Gabon by evaluating their vector competence for an African (i.e. representative of the endemic strains circulating in sub-Saharan Africa) and two Asian (i.e. representatives of exogenous epidemic strains that could be introduced) ZIKV strains. The transmission efficiency of one Ae. aegypti and two Ae. albopictus field-collected populations from Libreville and Franceville was assayed at day 7, 14 and 21 after experimental oral infection. The two mosquito species could transmit all three ZIKV strains already at day 7 post-infection, but transmission efficiency was higher for the African strain than the non-African strains (>60% versus
Published: 2021
Full Text: View/download PDF

32. Serological evidence of West Nile virus infection in human populations and domestic birds in the Northwest of Morocco

Author: M’hammed Sarih, Sayeh Ezzikouri, Moussa Dia, Sylvie Lecollinet, Soukaina Arich, Anna-Bella Failloux, Cécile Beck, Najlaa Assaid, Soumaya Benjelloun, Khadija Akarid, Oumar Faye, Laboratoire de Parasitologie et Maladies Vectorielles [Maroc], Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université Hassan II [Casablanca] (UH2MC), Laboratoire des Hépatites Virales [Casablanca, Maroc] (LHV), Département de virologie - Virology Department [Dakar], Institut Pasteur de Dakar, Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], This study was funded by Institut Pasteur of Morocco., École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Pasteur [Paris] (IP)
Subjects: viruses, [SDV]Life Sciences [q-bio], Serological evidence, Antibodies, Viral, medicine.disease_cause, Neutralization, Serology, 0403 veterinary science, 0302 clinical medicine, Seroepidemiologic Studies, Immunology and Allergy, MESH: Animals, virus diseases, MESH: Enzyme-Linked Immunosorbent Assay, 04 agricultural and veterinary sciences, General Medicine, 3. Good health, Morocco, Infectious Diseases, MESH: Birds, Antibody, West Nile virus, Human, 040301 veterinary sciences, 030231 tropical medicine, Immunology, Enzyme-Linked Immunosorbent Assay, Biology, Microbiology, Birds, 03 medical and health sciences, Bird, Culex pipiens, medicine, Animals, Humans, Seroprevalence, MESH: West Nile virus, MESH: West Nile Fever, West Nile Virus Infection, MESH: Humans, MESH: Seroepidemiologic Studies, General Veterinary, biology.organism_classification, Virology, MESH: Morocco, biology.protein, West Nile Fever, Virus neutralization test, MESH: Antibodies, Viral
Abstract: International audience; West Nile virus (WNV) was recently detected in Culex pipiens mosquitoes in Morocco. The aim of this study was to evaluate the seroprevalence of WNV in humans and in domestic birds in two regions of Morocco by the detection of IgG antibodies. Blood samples were obtained from 91 human patients and 92 domestic birds from September to December 2019. All study samples were tested using competitive enzyme-linked immunosorbent assay (cELISA) and WNV neutralization tests (VNT) were performed on positive sera. Of all samples, 4 (4.39 %) humans and 4 (4.34 %) birds were found to be seropositive for flaviviruses by the cELISA test. The VNT revealed that three of the four human samples detected positive by cELISA contained neutralizing antibodies against WNV. Two bird samples were confirmed positive by VNT. These results show a significant seroprevalence of anti-WNV antibodies and therefore suggest the active circulation and exposure of human and bird populations in the northwest of Morocco.
Published: 2021
Full Text: View/download PDF

33. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva

Author: Fourniol, Lisa, Madec, Yoann, Mousson, Laurence, Vazeille, Marie, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), This study was supported by the European Union’s Horizon 2020 research and innovation program under EVAg grant agreement no. 653316 and the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID)., We thank Malika Hocine and Jocelyne Alexandre for helping in rearing mosquitoes., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)
Subjects: RNA viruses, Physiology, viruses, [SDV]Life Sciences [q-bio], Disease Vectors, Pathology and Laboratory Medicine, Mosquitoes, Medical Conditions, Filter Paper, Animal Products, Aedes, Medicine and Health Sciences, Chikungunya Virus, Reverse Transcriptase Polymerase Chain Reaction, Eukaryota, virus diseases, Agriculture, Honey, Body Fluids, Insects, Laboratory Equipment, Infectious Diseases, Blood, Medical Microbiology, Viral Pathogens, Viruses, Engineering and Technology, RNA, Viral, Medicine, Pathogens, Anatomy, Salivation, Research Article, Paper, Arthropoda, Alphaviruses, Science, education, Equipment, Aedes Aegypti, Microbiology, Specimen Handling, Togaviruses, parasitic diseases, Animals, Saliva, Microbial Pathogens, Nutrition, fungi, Organisms, Biology and Life Sciences, Invertebrates, Insect Vectors, Diet, Species Interactions, Food, Physiological Processes, Zoology, Entomology
Abstract: International audience; Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
Published: 2021
Full Text: View/download PDF

34. Developing a Vaccine to Block West Nile Virus Transmission: In Silico Studies, Molecular Characterization, Expression, and Blocking Activity of Culex pipiens mosGCTL-1

Author: Hasan, Bakhshi, Mehdi, Fazlalipour, Javad, Dadgar-Pakdel, Sedigheh, Zakeri, Abbasali, Raz, Anna-Bella, Failloux, Navid, Dinparast Djadid, Malaria and Vector Research Group (MVRG), Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Department of Arboviruses and Viral Hemorrhagic Fevers [Tehran], Tehran University of Medical Sciences (TUMS), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), H.B. received scholarships from the education office of Pasteur Institute of Iran, Institut Pasteur of Paris, and Campus France. This work was also supported by the MATI Program of the Institut Pasteur International Network 'ENVIRONmental changes and MOSquito-borne diseases: the example of West Nile (Environ-MOS)'., We would like to thank Arezoo Beig-Parikhani for her assistance in performing the protein structure prediction and interaction. We thank our colleagues in MVRG, National Insectary, and AIV for their support during performing this project., and Institut Pasteur [Paris]
Subjects: Culex pipiens, transmission blocking vaccine, viruses, mosGCTL-1, [SDV]Life Sciences [q-bio], lcsh:R, lcsh:Medicine, virus diseases, West Nile virus, Article
Abstract: Background: Mosquito galactose-specific C-type lectins (mosGCTLs), such as mosGCTL-1, act as ligands to facilitate the invasion of flaviviruses like West Nile virus (WNV). WNV interacts with the mosGCTL-1 of Aedes aegypti (Culicidae) and facilitates the invasion of this virus. Nevertheless, there is no data about the role of mosGCTL-1 as a transmission-blocking vaccine candidate in Culex pipiens, the most abundant Culicinae mosquito in temperate regions. Methods: Adult female Cx. pipiens mosquitoes were experimentally infected with a WNV infectious blood meal, and the effect of rabbit anti-rmosGCTL-1 antibodies on virus replication was evaluated. Additionally, in silico studies such as the prediction of protein structure, homology modeling, and molecular interactions were carried out. Results: We showed a 30% blocking activity of Cx. pipiens mosGCTL-1 polyclonal antibodies (compared to the 10% in the control group) with a decrease in infection rates in mosquitoes at day 5 post-infection, suggesting that there may be other proteins in the midgut of Cx. pipiens that could act as cooperative-receptors for WNV. In addition, docking results revealed that WNV binds with high affinity, to the Culex mosquito lectin receptors. Conclusions: Our results do not support the idea that mosGCTL-1 of Cx. pipiens primarily interacts with WNV to promote viral infection, suggesting that other mosGCTLs may act as primary infection factors in Cx. pipiens.
Published: 2021
Full Text: View/download PDF

35. Corrigendum : Le déclin des Insectes : il est urgent d’agir

Author: Yvon Le Maho, Jean-Dominique Lebreton, Jean-Luc Imler, Louis Lambrechts, Pascale Cossart, Philippe Grandcolas, Jean-Claude Duplessy, Anna-Bella Failloux, Hervé Jactel, Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Modèles Insectes de l'Immunité Innée (M3I), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Centre Scientifique de Monaco (CSM), Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris], Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), and Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE)
Subjects: 0106 biological sciences, 0303 health sciences, Ecology, media_common.quotation_subject, [SDV]Life Sciences [q-bio], General Medicine, Insect, Biology, Legend, 01 natural sciences, 03 medical and health sciences, Action (philosophy), Butterfly, 030304 developmental biology, 010606 plant biology & botany, media_common
Abstract: International audience; There is an error in legend of Figure 3, names of the butterfly species have been swapped and therefore do not correspond to the photos of these species.; Une erreur s’est glissée dans la légende de la Figure 3, avec une interversion des noms des espèces de papillons qui ne correspondent donc pas aux photos de ces espèces.
Published: 2021
Full Text: View/download PDF

36. Aspects moléculaires de l'influence de la température sur la transmission du virus du chikungunya par le moustique Aedes albopictus

Author: Bellone, Rachel, STAR, ABES, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Sorbonne Université, Anna-Bella Failloux, and Institut Pasteur [Paris] (IP)
Subjects: [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Transcriptional regulation, Bacterial microbiota, Régulation transcriptionnelle, Évolution virale, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Temperature, Microbiome bactérien, Aedes albopictus, Viral evolution, Virus du chikungunya, Température, Chikungunya virus
Abstract: The chikungunya virus (CHIKV) is an emerging mosquito-borne Alphavirus which has widely spread around the world in the last two decades. The virus is transmitted to human hosts by Aedes mosquitoes, including the invasive species Aedes albopictus, which has today conquered more than half of the French territory. As a vector of several viral pathogens, Ae. albopictus poses a real threat to the health authorities. The emergence of arboviruses such as CHIKV, often results from a complex combination of both intrinsic and extrinsic factors. Since mosquitoes are poikilothermic ectotherms (i.e., internal body temperature is not constant and depends on environmental temperatures), they are acutely susceptible to temperature variations. The relation between temperature and arbovirus transmission is a complex phenomenon that remains poorly understood, especially at the molecular level. The aim of our project is to better understand how temperature affects mosquito-virus interactions and influences transmission cycles. We study the molecular aspects of CHIKV, its vector Ae. albopictus and their interactions under the influence of temperature. Our results show that temperature affects CHIKV evolution as well as mosquito genetic expression and microbial composition, especially in response to infection. These data provide important information on how vector systems can be affected by temperature. Understanding the mechanisms underlying virus-mosquito interactions with the environment is essential in order to prevent epidemics., Le virus du chikungunya (CHIKV) est un arbovirus émergeant qui, au cours des dernières décennies, s’est largement propagé à l’échelle mondiale. Le virus est transmis par les moustiques du genre Aedes, notamment Aedes albopictus qui est aujourd’hui présent dans une soixantaine de départements en France. Vecteurs de plusieurs agents pathogènes, Ae. albopictus représente une réelle menace de santé publique. L’émergence d’arboviroses est généralement liée à la convergence d’un ensemble de facteurs intrinsèques et extrinsèques affectant le vecteur, l’agent pathogène et l’hôte. Le moustique étant un organisme ectotherme, dont la température interne varie avec celle de l’environnement, il est très sensible aux variations de température du milieu ambiant. La relation entre la température et la transmission des arbovirus reste encore mal comprise, en particulier sur le plan moléculaire. L’objectif général de ce projet est de comprendre comment la température affecte les interactions virus-moustique et influence les cycles de transmission. Pour cela, nous étudions les aspects moléculaires du CHIKV, de son vecteur Ae. albopictus et de leurs interactions sous l’influence de la température. Nos résultats démontrent que la température affecte d’une part l’évolution du CHIKV et d’autre part, l’expression génétique et la composition microbienne du moustique, notamment en réponse à l’infection. Ces données apportent des informations importantes sur la manière dont les systèmes vectoriels peuvent être affectés par la température. La compréhension des mécanismes sous-jacents les interactions virus-moustique avec l’environnement sont essentielles afin de prévenir les épidémies.
Published: 2021

37. Favipiravir Does Not Inhibit Chikungunya Virus Replication in Mosquito Cells and Aedes aegypti Mosquitoes

Author: Ria Van Berwaer, Lieve Naesens, Anna-Bella Failloux, Leen Delang, Evelien Vanderlinden, Ana Lucia Rosales Rosas, Lanjiao Wang, Sofie Jacobs, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), This research was funded by an FWO Research Grant to L.D. and a KU Leuven C2 grant (C22/18/007). The work is supported by the project, Research Infrastructures for the control of vector-borne diseases (Infravec2), which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement number 731060. S.J. is funded by a SB-PhD scholarship from FWO, Flanders, Belgium., European Project: 731060,INFRAVEC2(2017), and Institut Pasteur [Paris]
Subjects: 0301 basic medicine, Microbiology (medical), QH301-705.5, medicine.drug_class, [SDV]Life Sciences [q-bio], 030106 microbiology, Aedes aegypti, favipiravir, Favipiravir, medicine.disease_cause, Microbiology, Arbovirus, 03 medical and health sciences, Virology, parasitic diseases, medicine, Chikungunya, Biology (General), mosquitoes, Aedes, chikungunya virus, biology, fungi, virus diseases, medicine.disease, biology.organism_classification, 3. Good health, 030104 developmental biology, Viral replication, antiviral activity, Vero cell, T-705, activation, Antiviral drug
Abstract: Favipiravir (T-705) is a broad-spectrum antiviral drug that inhibits RNA viruses after intracellular conversion into its active form, T-705 ribofuranosyl 5'-triphosphate. We previously showed that T-705 is able to significantly inhibit the replication of chikungunya virus (CHIKV), an arbovirus transmitted by Aedes mosquitoes, in mammalian cells and in mouse models. In contrast, the effect of T-705 on CHIKV infection and replication in the mosquito vector is unknown. Since the antiviral activity of T-705 has been shown to be cell line-dependent, we studied here its antiviral efficacy in Aedes-derived mosquito cells and in Aedes aegypti mosquitoes. Interestingly, T-705 was devoid of anti-CHIKV activity in mosquito cells, despite being effective against CHIKV in Vero cells. By investigating the metabolic activation profile, we showed that, unlike Vero cells, mosquito cells were not able to convert T-705 into its active form. To explore whether alternative metabolization pathways might exist in vivo, Aedes aegypti mosquitoes were infected with CHIKV and administered T-705 via an artificial blood meal. Virus titrations of whole mosquitoes showed that T-705 was not able to reduce CHIKV infection in mosquitoes. Combined, these in vitro and in vivo data indicate that T-705 lacks antiviral activity in mosquitoes due to inadequate metabolic activation in this animal species. ispartof: MICROORGANISMS vol:9 issue:5 ispartof: location:Switzerland status: published
Published: 2021
Full Text: View/download PDF

38. Evidence For Long-Lasting Transgenerational Antiviral Immunity in Insects

Author: Anna-Bella Failloux, Lionel Frangeul, Maria-Carla Saleh, Marie Vazeille, Valérie Gausson, Juan A Mondotte, Vanesa Mongelli, Yasutsugu Suzuki, Hervé Blanc, Virus et Interférence ARN - Viruses and RNA Interference, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], This work was supported by the European Research Council (FP7/2013-2019 ERC CoG 615220) and the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID) to M.-C.S. J.A.M. was supported by AXA Research Fund., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 615220,EC:FP7:ERC,ERC-2013-CoG,RNAIMMUNITY(2015), European Project: 9418559(1995), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
Subjects: 0301 basic medicine, RNA viruses, Insecta, Biology, Antiviral Agents, Article, General Biochemistry, Genetics and Molecular Biology, Virus, immune response, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Aedes aegypti, Aedes, RNA interference, Animals, insects, Gene, lcsh:QH301-705.5, Genetics, RNA, chromatin modifications, transgenerational immune priming, adaptive immunity, Acquired immune system, 3. Good health, Chromatin, 030104 developmental biology, Drosophila melanogaster, chemistry, lcsh:Biology (General), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, antiviral memory, Immunologic Memory, 030217 neurology & neurosurgery, DNA
Abstract: Summary Transgenerational immune priming (TGIP) allows memory-like immune responses to be transmitted from parents to offspring in many invertebrates. Despite increasing evidence for TGIP in insects, the mechanisms involved in the transfer of information remain largely unknown. Here, we show that Drosophila melanogaster and Aedes aegypti transmit antiviral immunological memory to their progeny that lasts throughout generations. We observe that TGIP, which is virus and sequence specific but RNAi independent, is initiated by a single exposure to disparate RNA viruses and also by inoculation of a fragment of viral double-stranded RNA. The progeny, which inherit a viral DNA that is only a fragment of the viral RNA used to infect the parents, display enriched expression of genes related to chromatin and DNA binding. These findings represent a demonstration of TGIP for RNA viruses in invertebrates, broadly increasing our understanding of the immune response, host genome plasticity, and antiviral memory of the germline., Graphical Abstract, Highlights • Drosophila and mosquitoes transmit antiviral immunological memory to their progeny • Protection is virus and sequence specific and lasts throughout generations • Progeny inherit a viral DNA form that is a partial copy of the RNA virus genome • Progeny display enriched expression of genes related to chromatin and DNA binding, Mondotte et al. determine that antiviral transgenerational immune priming in Drosophila and mosquitoes occurs after parental priming with different single-stranded RNA viruses. The progeny are protected from infection with the same virus for several generations.
Published: 2020
Full Text: View/download PDF

39. The recently introduced Aedes albopictus in Tunisia has the potential to transmit chikungunya, dengue and Zika viruses

Author: Anna-Bella Failloux, Adel Rhim, Ali Bouattour, Yoann Madec, Marie Vazeille, Laurence Mousson, Chloé Bohers, Youmna M’ghirbi, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), This study was funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No 731060 (Infravec2, Research Infrastructures for the control of vector-borne diseases, We thank Dr Boubidi Said (Institut Pasteur of Algeria) for critical discussion., European Project: 731060,INFRAVEC2(2017), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)
Subjects: RNA viruses, Male, 0301 basic medicine, Viral Diseases, Physiology, viruses, [SDV]Life Sciences [q-bio], RC955-962, Mosquito population, Mosquito saliva, Disease Vectors, Dengue virus, Pathology and Laboratory Medicine, medicine.disease_cause, Mosquitoes, Dengue fever, Geographical Locations, Dengue, Medical Conditions, 0302 clinical medicine, Aedes, Arctic medicine. Tropical medicine, Abdomen, Chlorocebus aethiops, Medicine and Health Sciences, Chikungunya, Chikungunya Virus, Zika Virus Infection, Eukaryota, virus diseases, Viral Load, Body Fluids, 3. Good health, Insects, Infectious Diseases, Medical Microbiology, Viral Pathogens, Viruses, Female, Rabbits, Pathogens, Anatomy, Public aspects of medicine, RA1-1270, Viral load, Research Article, Neglected Tropical Diseases, Tunisia, Aedes albopictus, Arthropoda, Alphaviruses, 030231 tropical medicine, Mosquito Vectors, Biology, Microbiology, Virus, Cell Line, Togaviruses, 03 medical and health sciences, Virology, medicine, Animals, Saliva, Microbial Pathogens, Vero Cells, Flaviviruses, fungi, Organisms, Public Health, Environmental and Occupational Health, Biology and Life Sciences, Chikungunya Infection, Zika Virus, Dengue Virus, Tropical Diseases, medicine.disease, biology.organism_classification, Invertebrates, Insect Vectors, Species Interactions, 030104 developmental biology, People and Places, Africa, Chikungunya Fever, Zoology, Entomology, Viral Transmission and Infection
Abstract: The mosquito Aedes albopictus was detected for the first time in Tunisia in 2018. With its establishment in the capital city of Tunis, local health authorities fear the introduction of new human arboviral diseases, like what happened in Europe with unexpected local cases of chikungunya, dengue and Zika. Even though this mosquito is competent to transmit the arboviruses mentioned above, the transmission level will vary depending on the couple, mosquito population and virus genotype. Here, we assessed the vector competence of Ae. albopictus Tunisia by experimental infections with chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses. We found that Ae. albopictus Tunisia was highly competent for CHIKV (transmission efficiency of 25% at 21 post-infection) and to a lesser extent, for ZIKV (8.7%) and DENV (8.3%). Virus was detected in mosquito saliva at day 3 (CHIKV), day 10 (ZIKV) and day 21 (DENV) post-infection. These results suggest that the risk of emergence of chikungunya is the highest imposing a more sustained surveillance to limit Ae. albopictus populations in densely populated urban dwellings and at the entry points of travelers returning from CHIKV-endemic regions., Author summary After Algeria and Morocco, the mosquito Aedes albopictus was detected in 2018 in Tunisia. This invasive species has continued its global expansion and then, extended the fear of introducing Aedes-transmitted arboviral diseases in countries where people are immunologically naïve. We demonstrated, using experimental infections, that Ae. albopictus mosquitoes from Tunisia were more competent to chikungunya virus than to Zika virus and dengue virus. These results are critical to design a control strategy targeting Ae. albopictus populations in regions hosting travelers returning from arboviral-endemic regions.
Published: 2020
Full Text: View/download PDF

40. The Role of Temperature in Shaping Mosquito-Borne Viruses Transmission

Author: Anna-Bella Failloux, Rachel Bellone, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Sorbonne Université (SU), This study was supported by the European Union’s Horizon 2020 Research and Innovation Program under grant agreement no. 731060 (Infravec2, Research Infrastructures for the Control of Vector-Borne Diseases, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 731060,INFRAVEC2(2017), European Project: 734548,ZIKAlliance(2016), and Institut Pasteur [Paris]
Subjects: Abiotic component, Microbiology (medical), 0303 health sciences, 030306 microbiology, Ecology, Transmission (medicine), lcsh:QR1-502, temperature, Mosquito-borne viruses, Review, Biology, vectorial capacity, Microbiology, lcsh:Microbiology, 3. Good health, 03 medical and health sciences, Human health, 13. Climate action, arboviruses, vector-borne diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 030304 developmental biology, mosquitoes
Abstract: International audience; Mosquito-borne diseases having the greatest impact on human health are typically prevalent in the tropical belt of the world. However, these diseases are conquering temperate regions, raising the question of the role of temperature on their dynamics and expansion. Temperature is one of the most significant abiotic factors affecting, in many ways, insect vectors and the pathogens they transmit. Here, we debate the veracity of this claim by synthesizing current knowledge on the effects of temperature on arboviruses and their vectors, as well as the outcome of their interactions.
Published: 2020
Full Text: View/download PDF

41. Genetic evidence for the origin of Aedes aegypti , the yellow fever mosquito, in the southwestern Indian Ocean

Author: Vincent Robert, John Soghigian, Anna-Bella Failloux, Andrea Gloria-Soria, Jeffrey R. Powell, Gilbert Le Goff, Yale University [New Haven], North Carolina State University [Raleigh] (NC State), University of North Carolina System (UNC), Connecticut Agricultural Experiment Station, Partenaires INRAE, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), The funding for the SNP chip used in this study came from NIH award RO1 AI101112. JS was partially funded on NSF DEB #1754376 and AG‐S by the Grant or Cooperative Agreement Number U01CK000509, funded by the Centers for Disease Control and Prevention., and Institut Pasteur [Paris]
Subjects: 0106 biological sciences, 0301 basic medicine, Systematics, Species complex, Asia, education, Zoology, Introgression, Mosquito Vectors, Aedes aegypti, Biology, Subspecies, Population and Conservation Genetics, 010603 evolutionary biology, 01 natural sciences, invasive species, 03 medical and health sciences, Aedes, Yellow Fever, Madagascar, Genetics, medicine, Animals, Humans, insects, Clade, systematics, Indian Ocean, Ecology, Evolution, Behavior and Systematics, population genetics—empirical, Yellow fever, fungi, virus diseases, biology.organism_classification, medicine.disease, population genetics-empirical, 030104 developmental biology, Vector (epidemiology), Africa, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Original Article, ORIGINAL ARTICLES, Reunion
Abstract: Aedes aegypti is among the best‐studied mosquitoes due to its critical role as a vector of human pathogens and ease of laboratory rearing. Until now, this species was thought to have originated in continental Africa, and subsequently colonized much of the world following the establishment of global trade routes. However, populations of this mosquito on the islands in the southwestern Indian Ocean (SWIO), where the species occurs with its nearest relatives referred to as the Aegypti Group, have received little study. We re‐evaluated the evolutionary history of Ae. aegypti and these relatives, using three data sets: nucleotide sequence data, 18,489 SNPs and 12 microsatellites. We found that: (a) the Aegypti Group diverged 16 MYA (95% HPD: 7–28 MYA) from its nearest African/Asian ancestor; (b) SWIO populations of Ae. aegypti are basal to continental African populations; (c) after diverging 7 MYA (95% HPD: 4–15 MYA) from its nearest formally described relative (Ae. mascarensis), Ae. aegypti moved to continental Africa less than 85,000 years ago, where it recently (
Published: 2020
Full Text: View/download PDF

42. Serological evidence of West Nile virus infection among birds and horses in some geographical locations of Iran

Author: Navid Dinparast-Djadid, Leila Nourani, Abbasali Raz, Anna-Bella Failloux, Cécile Beck, Maëlle Monier, Sylvie Lecollinet, Kourosh Arzamani, Sedigheh Zakeri, Hasan Bakhshi, Laurence Mousson, Malaria and Vector Research Group (MVRG), Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], North Khorasan University of Medical Sciences [Iran], This work was supported by the MATI Program of the Institut Pasteur International Network 'ENVIRONmental changes and MOSquito‐borne diseases: the example of West Nile (Environ‐MOS)'. HB, as a Ph.D. student of medical biotechnology also received scholarships from education office of IP Iran, IP Paris and Campus France., We appreciate Dr. Mehdi Fazlalipour and Dr. Sadegh Chinikar (Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran (IPI), Tehran, Iran) for their valuable supports which worth to be regarded as co‐authors. The authors are grateful to the staff of Public Health Department, North Khorasan University of Medical Sciences (North Khorasan, Iran) and North Research Center of IPI (Amol, Iran). We also thank Mr. Kheiri for his help during the project. We would also like to express our sincere appreciation to the local inhabitants in the study areas., École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Pasteur [Paris] (IP)
Subjects: Eagle, Veterinary medicine, viruses, 030231 tropical medicine, Animals, Wild, Enzyme-Linked Immunosorbent Assay, Iran, Serology, Birds, 03 medical and health sciences, 0302 clinical medicine, vertebrate hosts, Seroepidemiologic Studies, biology.animal, Prevalence, medicine, Animals, Seroprevalence, Horses, Usutu virus, 0303 health sciences, West Nile Virus Infection, General Veterinary, biology, Bird Diseases, 030306 microbiology, virus diseases, Original Articles, medicine.disease, biology.organism_classification, 3. Good health, Buzzard, Flavivirus, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Horse Diseases, Original Article, ELISA, West Nile virus, West Nile Fever, Encephalitis
Abstract: Recent expansion of arboviruses such as West Nile (WNV), Usutu (USUV), and tick‐borne encephalitis (TBEV) over their natural range of distribution needs strengthening their surveillance. As common viral vertebrate hosts, birds and horses deserve special attention with routine serological surveillance. Here, we estimated the seroprevalence of WNV, USUV and TBEV in 160 migrating/resident birds and 60 horses sampled in Mazandaran, Golestan, North Khorasan, Kordestan provinces and Golestan province of Iran respectively. ELISA results showed that of 220 collected samples, 32 samples (14.54%), including 22 birds and 10 horses, were positive. Microsphere immunoassay results showed that 16.7% (10/60) of horse blood samples collected in Golestan province were seropositive against WNV (7; 11.7%), Flavivirus (2; 3.3%) and seropositive for USUV or WNV (1; 1.7%). Furthermore, micro virus neutralization tests revealed that four of seven ELISA‐positive bird blood samples were seropositive against WNV: two Egyptian vultures, and one long‐legged buzzard collected in Golestan province as well as a golden eagle collected in North Khorasan province. No evidence of seropositivity with TBEV was observed in collected samples. We showed that WNV, responsible for neuroinvasive infection in vertebrates, is circulating among birds and horses in Iran, recommending a sustained surveillance of viral infections in animals, and anticipating future infections in humans., We estimated the seroprevalence of WNV, USUV and TBEV in 160 birds and 60 horses. ELISA results showed that 32 samples were positive: Microsphere immunoassay results showed that 16.7% of horse blood samples were seropositive against WNV, Flavivirus and seropositive for USUV or WNV. Furthermore, micro virus neutralization tests revealed that four of seven ELISA‐positive bird blood samples were seropositive against WNV.
Published: 2020
Full Text: View/download PDF

43. Detection of arboviruses in mosquitoes: Evidence of circulation of chikungunya virus in Iran

Author: Marie Vazeille, Géraldine Piorkowski, Hasan Bakhshi, Sara Moutailler, Xavier de Lamballerie, Navid Dinparast-Djadid, Laurence Mousson, Anna-Bella Failloux, Sedigheh Zakeri, Abbasali Raz, Malaria and Vector Research Group (MVRG), Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), European Project: 734548,ZIKAlliance(2016), Noel, Anne-Laure, A global alliance for Zika virus control and prevention - ZIKAlliance - 2016-10-01 - 2019-09-30 - 734548 - VALID, Institut Pasteur [Paris], École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), and École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé
Subjects: 0301 basic medicine, Male, RNA viruses, Viral Diseases, viruses, RC955-962, Dengue virus, Iran, Disease Vectors, medicine.disease_cause, Pathology and Laboratory Medicine, Mosquitoes, Geographical Locations, Human health, 0302 clinical medicine, Arctic medicine. Tropical medicine, Genotype, Screening method, Medicine and Health Sciences, Chikungunya, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Chikungunya Virus, biology, virus diseases, Eukaryota, 3. Good health, Insects, Infectious Diseases, Medical Microbiology, Arboviral Infections, Viral Pathogens, Viruses, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, Public aspects of medicine, RA1-1270, Pathogens, West Nile virus, Research Article, Neglected Tropical Diseases, Asia, Arthropoda, Alphaviruses, 030231 tropical medicine, Microbiology, Virus, Togaviruses, 03 medical and health sciences, Culex pipiens, parasitic diseases, medicine, Animals, Microbial Pathogens, Flaviviruses, Public Health, Environmental and Occupational Health, Organisms, Biology and Life Sciences, Chikungunya Infection, Dengue Virus, biology.organism_classification, Tropical Diseases, Virology, Invertebrates, Insect Vectors, Species Interactions, 030104 developmental biology, Culicidae, People and Places, Anopheles maculipennis, Arboviruses
Abstract: Mosquitoes are vectors of viruses affecting animal and human health. In Iran, the prevalence of mosquito-borne viruses remains poorly investigated. Once infected, mosquito females remain infected for all their life making virus detections possible at early steps before infections are reported in vertebrate hosts. In this study, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system capable of detecting 37 arboviruses in a single experiment. A total of 1,212 mosquitoes collected in Mazandaran, North-Khorasan, and Fars provinces of Iran were analyzed. Eighteen species were identified, belonging to five genera; the most prevalent species were Anopheles maculipennis s.l. (42.41%), Culex pipiens (19.39%), An. superpictus (11.72%), and Cx. tritaeniorhynchus (10.64%). We detected chikungunya virus (CHIKV) of the Asian genotype in six mosquito pools collected in North Khorasan and Mazandaran provinces. To our knowledge, this is the first report of mosquitoes infected with CHIKV in Iran. Our high-throughput screening method can be proposed as a novel epidemiological surveillance tool to identify circulating arboviruses and to support preparedness to an epidemic in animals and humans., Author summary Mosquito-borne viruses pose a serious threat to public health worldwide. For an efficient preparedness to future emergences, an early detection of arboviruses in vector populations would be wise before animal/human cases arise. Here, we used a recently developed high-throughput chip based on the BioMark Dynamic arrays system, capable of screening various arboviruses in a single experiment. By examining 1,212 mosquitoes collected in Iran, we provide evidence that chikungunya virus is circulating in Iran.
Published: 2020
Full Text: View/download PDF

44. Vector competence of Aedes albopictus populations for chikungunya virus is shaped by their demographic history

Author: Anubis Vega-Rúa, Mosè Manni, Anna-Bella Failloux, Yoann Madec, Michele Marconcini, Anna R. Malacrida, Giuliano Gasperi, Ludvik M. Gomulski, Davide Carraretto, Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP), Università degli Studi di Pavia = University of Pavia (UNIPV), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Université de Genève = University of Geneva (UNIGE), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Università degli Studi di Pavia, Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), University of Geneva [Switzerland], University of Pavia, and Institut Pasteur [Paris]
Subjects: 0301 basic medicine, Entomology, Aedes albopictus, Demographic history, viruses, 030231 tropical medicine, education, Medicine (miscellaneous), Zoology, Mosquito Vectors, Biology, medicine.disease_cause, Arbovirus, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Aedes, Genetic variation, medicine, Animals, Chikungunya, lcsh:QH301-705.5, Asia, Southeastern, Genetic Variation, Outbreak, virus diseases, South America, medicine.disease, biology.organism_classification, 3. Good health, Genetics, Population, 030104 developmental biology, lcsh:Biology (General), Vector (epidemiology), Host-Pathogen Interactions, North America, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Chikungunya Fever, Female, Introduced Species, General Agricultural and Biological Sciences, Chikungunya virus, Microsatellite Repeats
Abstract: The mosquito Aedes albopictus is one of the most dangerous invasive species. Its worldwide spread has created health concerns as it is a major vector of arboviruses of public health significance such as chikungunya (CHIKV). Dynamics of different genetic backgrounds and admixture events may have impacted competence for CHIKV in adventive populations. Using microsatellites, we infer the genetic structure of populations across the expansion areas that we then associate with their competence for different CHIKV genotypes. Here we show that the demographic history of Ae. albopictus populations is a consequence of rapid complex patterns of historical lineage diversification and divergence that influenced their competence for CHIKV. The history of adventive populations is associated with CHIKV genotypes in a genotype-by-genotype interaction that impacts their vector competence. Thus, knowledge of the demographic history and vector competence of invasive mosquitoes is pivotal for assessing the risk of arbovirus outbreaks in newly colonized areas., Anubis Vega-Rúa, Michele Marconcini et al. study how the dynamics of genetics and admixture events of Aedes albopictus affect its competence for the CHIKV. Using microsatellites, they found that the demographic history influences the competence for the virus and is an important feature to assess the risk of outbreaks in new areas.
Published: 2020
Full Text: View/download PDF

45. Arbo France : a French network for the study of human and animal arboviruses

Author: Murgue, Bernadette, Failloux, Anna, de Lamballerie, Xavier, Unité des Virus Emergents (UVE), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], This network has received funding from the multidisciplinary 'Reacting' consortium coordinated by INSERM (French National Institute of Health and Medical Research) on behalf of all partners of AVIESAN (French National Alliance for Life Sciences and Health)., Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut Pasteur [Paris] (IP)
Subjects: [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2020
Full Text: View/download PDF

46. Assessing entomological risk factors for arboviral disease transmission in the French Territory of the Wallis and Futuna Islands

Author: Elodie Calvez, Nicolas Pocquet, Atoloto Malau, Sosiasi Kilama, Alefosio Taugamoa, Didier Labrousse, Philippe Boussès, Anna-Bella Failloux, Myrielle Dupont-Rouzeyrol, Françoise Mathieu-Daudé, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Service de l'Environnement - Préfecture des îles Wallis et Futuna, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Vector Control Group (MIVEGEC-VCG), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), This work was funded by the French Ministère des Outre-mer, DGOM (HC/DAIRCL/2180-310) (FMD) and partly supported by the Actions Concertées Inter Pasteuriennes (ZikAe project, ACIP A-15-2014) (ABF and MDR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., and Institut Pasteur [Paris] (IP)
Subjects: RNA viruses, Topography, Viral Diseases, RC955-962, Disease Vectors, Pathology and Laboratory Medicine, Mosquitoes, Geographical locations, Aedes, Arctic medicine. Tropical medicine, Surveys and Questionnaires, Medicine and Health Sciences, Prospective Studies, Islands, Chikungunya Virus, Eukaryota, Insects, Infectious Diseases, Medical Microbiology, Arboviral Infections, Viral Pathogens, Viruses, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Female, Public aspects of medicine, RA1-1270, Pathogens, Research Article, Neglected Tropical Diseases, Arthropoda, Alphaviruses, Oceania, education, Mosquito Vectors, Aedes Aegypti, Risk Assessment, Microbiology, Polynesia, Togaviruses, Disease Transmission, Infectious, Animals, Microbial Pathogens, Ecosystem, Landforms, Organisms, Biology and Life Sciences, Chikungunya Infection, Geomorphology, Wallis and Futuna, Tropical Diseases, Invertebrates, Insect Vectors, Species Interactions, Earth Sciences, Chikungunya Fever, People and places, Arboviruses
Abstract: Background The French overseas Territory of the Wallis and Futuna Islands has been affected by several dengue epidemics. Aedes polynesiensis is the main mosquito vector described in this territory. Other Aedes species have been reported, but recent entomological data are missing to infer the presence of other potential arbovirus vectors and to assess the entomological risk factors for transmission of arboviral diseases. Methodology/ Principal findings An entomological prospective study was conducted on the three main islands of the territory to determine the presence and distribution of Aedes spp. Larvae, pupae and adult mosquitoes were collected from 54 sampling points in different environments, with a final sampling of 3747 immature stages and 606 adults. The main identified breeding sites were described. Ae. polynesiensis was found in every sampled site in peridomestic and wild habitats. Ae. aegypti was only found on the island of Wallis in peridomestic environments with a limited distribution. Two other Aedes species endemic to the Pacific were recorded, Aedes oceanicus and Aedes futunae. To evaluate the ability of local Ae. polynesiensis to transmit the chikungunya virus (CHIKV), two field populations were analyzed for vector competence using experimental oral exposure of females to CHIKV and infection, dissemination and transmission assays. Results showed that both populations of Ae. polynesiensis were competent for CHIKV (30% at 7 days post-infection). Conclusions/Significance This study showed the ubiquitous distribution and abundance of Ae. polynesiensis on the three islands and demonstrated that local populations were able to transmit CHIKV. Combined with the presence and expansion of Ae. aegypti on the main island of Wallis, these data highlight the risk of transmission of arboviral diseases in the territory of Wallis and Futuna and provide relevant information for entomological surveillance and vector control programs., Author summary The French overseas Territory of the Wallis and Futuna Islands, located in the South Pacific, has been affected by several dengue epidemics, but did not face Zika or chikungunya outbreaks, unlike other neighboring islands. The near-exclusive presence of the Aedes polynesiensis mosquito in the islands of Wallis and Futuna confirmed the role played by this mosquito as a vector of dengue fever. A local Ae. polynesiensis population was recently shown to be able to transmit the Zika virus under experimental conditions, but its susceptibility to the chikungunya virus was still unknown, and recent data on the presence of other potential arbovirus vectors were missing. Therefore, we investigated the entomological risk factors for the transmission of arboviral diseases in the Wallis and Futuna Islands. We reported the occurrence and distribution of different Aedes species, especially the abundant presence of Ae. polynesiensis across the territory and the spread of Ae. aegypti in the island of Wallis. Our results demonstrated the ability of local Ae. polynesiensis populations to transmit the chikungunya virus. These findings highlight the risk of arbovirus transmission in the Wallis and Futuna Islands and provide relevant data to guide prevention and vector control strategies in the territory.
Published: 2020
Full Text: View/download PDF

47. A Review: Wolbachia-Based Population Replacement for Mosquito Control Shares Common Points with Genetically Modified Control Approaches

Author: Anna-Bella Failloux, Pei-Shi Yen, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), This study was funded by the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases, IBEID' (grant n_ANR-10-LABX-62-IBEID) (A.-B.F.) and the National Health Research Institutes (NHRI-PP-108-0324-01-17-07) (P.-S.Y.)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris]
Subjects: Microbiology (medical), replacement strategy, Arbovirus Infections, 030231 tropical medicine, lcsh:Medicine, Population Replacement, Arbovirus, mosquito control, 03 medical and health sciences, Incomplete knowledge, 0302 clinical medicine, environmental factors, parasitic diseases, medicine, Immunology and Allergy, Wolbachia, Molecular Biology, 030304 developmental biology, Genetics, 0303 health sciences, General Immunology and Microbiology, biology, Host (biology), lcsh:R, medicine.disease, biology.organism_classification, viral adaptation, 3. Good health, Genetically modified organism, Mosquito control, Infectious Diseases, arbovirus, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Abstract: The growing expansion of mosquito vectors has made mosquito-borne arboviral diseases a global threat to public health, and the lack of licensed vaccines and treatments highlight the urgent need for efficient mosquito vector control. Compared to genetically modified control strategies, the intracellular bacterium Wolbachia, endowing a pathogen-blocking phenotype, is considered an environmentally friendly strategy to replace the target population for controlling arboviral diseases. However, the incomplete knowledge regarding the pathogen-blocking mechanism weakens the reliability of a Wolbachia-based population replacement strategy. Wolbachia infections are also vulnerable to environmental factors, temperature, and host diet, affecting their densities in mosquitoes and thus the virus-blocking phenotype. Here, we review the properties of the Wolbachia strategy as an approach to control mosquito populations in comparison with genetically modified control methods. Both strategies tend to limit arbovirus infections but increase the risk of selecting arbovirus escape mutants, rendering these strategies less reliable.
Published: 2020
Full Text: View/download PDF

48. Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes

Author: Parry, Rhys, Naccache, Fanny, Ndiaye, El Hadji, Fall, Gamou, Castelli, Ilaria, Lühken, Renke, Medlock, Jolyon, Cull, Benjamin, Hesson, Jenny C., Montarsi, Fabrizio, Failloux, Anna-Bella, Kohl, Alain, Schnettler, Esther, Diallo, Mawlouth, Asgari, Sassan, Dietrich, Isabelle, Becker, Stefanie C., University of Queensland [Brisbane], University of Veterinary Medicine [Hannover], Pôle Zoologie médicale [Dakar], Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Arbovirus et Virus de Fièvres Hémorragiques [Dakar, Sénégal], Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Universität Hamburg (UHH), Bernhard Nocht Institute for Tropical Medicine - Bernhard-Nocht-Institut für Tropenmedizin [Hamburg, Germany] (BNITM), Public Health England [Salisbury] (PHE), Uppsala University, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), MRC - University of Glasgow Centre for Virus Research, German Centre for Infection Research - partner site Hamburg-Lübeck-Borstel-Riems (DZIF), The Pirbright Institute, Biotechnology and Biological Sciences Research Council (BBSRC), Sassan Asgari is supported by the Australian Research Council, grant number DP190102048. Alain Kohl is supported by the UK Medical Research Council (MC_UU_12014/8). Rhys Parry is supported by a University of Queensland scholarship. Isabelle Dietrich is supported by the Biotechnology and Biological Sciences Research Council (grant numbers BBS/E/I/00007033 and BBS/E/I/00007035). Jenny C. Hesson is supported by the Swedish Society for Medical Research., Institut Pasteur [Paris], and Institute for Animal Health, the Pirbright Institute
Subjects: virus discovery, [SDV]Life Sciences [q-bio], MESH: RNA Interference, lcsh:QR1-502, MESH: Picornaviridae, MESH: Aedes, MESH: Molecular Sequence Annotation, Microbiology, lcsh:Microbiology, Mikrobiologi, Aedes vexans, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, MESH: RNA, Viral, RNAi, MESH: RNA, Small Interfering, iflavirus, MESH: Sequence Analysis, RNA, MESH: Animals, MESH: Mosquito Vectors, MESH: Genome, Viral, MESH: Computational Biology
Abstract: The inland floodwater mosquito Aedes vexans (Meigen, 1830) is a competent vector of numerous arthropod-borne viruses such as Rift Valley fever virus (Phenuiviridae) and Zika virus (Flaviviridae). Aedes vexans spp. have widespread Afrotropical distribution and are common European cosmopolitan mosquitoes. We examined the virome of Ae. vexans arabiensis samples from Bark&eacute, dji village, Senegal, with small RNA sequencing, bioinformatic analysis, and RT-PCR screening. We identified a novel 9494 nt iflavirus (Picornaviridae) designated here as Aedes vexans iflavirus (AvIFV). Annotation of the AvIFV genome reveals a 2782 amino acid polyprotein with iflavirus protein domain architecture and typical iflavirus 5&rsquo, internal ribosomal entry site and 3&rsquo, poly-A tail. Aedes vexans iflavirus is most closely related to a partial virus sequence from Venturia canescens (a parasitoid wasp) with 56.77% pairwise amino acid identity. Analysis of AvIFV-derived small RNAs suggests that AvIFV is targeted by the exogenous RNA interference pathway but not the PIWI-interacting RNA response, as ~60% of AvIFV reads corresponded to 21 nt Dicer&minus, 2 virus-derived small RNAs and the 24&ndash, 29 nt AvIFV read population did not exhibit a &ldquo, ping-pong&rdquo, signature. The RT-PCR screens of archival and current (circa 2011&ndash, 2020) Ae. vexans arabiensis laboratory samples and wild-caught mosquitoes from Bark&eacute, dji suggest that AvIFV is ubiquitous in these mosquitoes. Further, we screened wild-caught European Ae. vexans samples from Germany, the United Kingdom, Italy, and Sweden, all of which tested negative for AvIFV RNA. This report provides insight into the diversity of commensal Aedes viruses and the host RNAi response towards iflaviruses.
Published: 2020
Full Text: View/download PDF

49. The Aedes aegypti Domino Ortholog p400 Regulates Antiviral Exogenous Small Interfering RNA Pathway Activity and ago-2 Expression

Author: Isabelle Dietrich, Emilie Pondeville, Floriane Almire, Claire L. Donald, Sandra Terry, Rommel J. Gestuveo, Margus Varjak, Esther Schnettler, Alain Kohl, Marie Vazeille, Melanie McFarlane, Mathilde Laureti, Alma McDonald, Colin Loney, Bryan A. Wee, Joy Kean, Anna-Bella Failloux, MRC - University of Glasgow Centre for Virus Research, University of Edinburgh, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], University of the Philippines (UP System), This work was funded by the UK Medical Research Council (grant MC_UU_12014/8 to A.K., E.S., and E.P.), Institut Pasteur (to M. Vazeille and A.-B.F.), and the French Government’s Investissements d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant ANR-10-LABX-62-IBEID to A.-B.F.). R.J.G. was supported by a British Council Newton Fund grant, 279705176, under the DOST-Newton Ph.D. Scholarship partnership, the grant is funded by the UK Department for Business, Energy and Industrial Strategy, Philippines Department of Science and Technology-Science Education Institute, and the University of the Philippines Visayas and delivered by the British Council. For further information, please visit http://www.newtonfund.ac.uk/., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
Subjects: 0301 basic medicine, Small interfering RNA, viruses, 030106 microbiology, lcsh:QR1-502, p400, mosquito, Mosquito Vectors, Alphavirus, Aedes aegypti, Semliki Forest virus, Microbiology, lcsh:Microbiology, Host-Microbe Biology, 03 medical and health sciences, RNA interference, Aedes, Animals, RNA, Small Interfering, Molecular Biology, innate immunity, biology, fungi, RNA, virus diseases, biology.organism_classification, Virology, QR1-502, 3. Good health, ago-2, 030104 developmental biology, arbovirus, Gene Expression Regulation, Argonaute Proteins, Togaviridae, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Insect Proteins, Female, Arboviruses, Research Article
Abstract: Female Aedes aegypti mosquitoes are vectors of human-infecting arthropod-borne viruses (arboviruses). In recent decades, the incidence of arthropod-borne viral infections has grown dramatically. Vector competence is influenced by many factors, including the mosquito’s antiviral defenses. The exogenous small interfering RNA (siRNA) pathway is a major antiviral response restricting arboviruses in mosquitoes. While the roles of the effectors of this pathway, Argonaute-2 and Dicer-2 are well characterized, nothing is known about its regulation in mosquitoes. In this study, we demonstrate that A. aegypti p400, whose ortholog Domino in Drosophila melanogaster is a chromatin-remodeling ATPase member of the Tip60 complex, regulates siRNA pathway activity and controls ago-2 expression levels. In addition, we found p400 to have antiviral activity against different arboviruses. Therefore, our study provides new insights into the regulation of the antiviral response in A. aegypti mosquitoes., Arboviruses are pathogens of humans and animals. A better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. A major antiviral pathway in the mosquito vector is the exogenous small interfering RNA (exo-siRNA) pathway, which is induced by arbovirus-derived double-stranded RNA in infected cells. Although recent work has shown the key role played by Argonaute-2 (Ago-2) and Dicer-2 (Dcr-2) in this pathway, the regulatory mechanisms that govern these pathways have not been studied in mosquitoes. Here, we show that the Domino ortholog p400 has antiviral activity against the alphavirus Semliki Forest virus (Togaviridae) both in Aedes aegypti-derived cells and in vivo. Antiviral activity of p400 was also demonstrated against chikungunya virus (Togaviridae) and Bunyamwera virus (Peribunyaviridae) but not Zika virus (Flaviviridae). p400 was found to be expressed across mosquito tissues and regulated ago-2 but not dcr-2 transcript levels in A. aegypti mosquitoes. These findings provide novel insights into the regulation of an important aedine exo-siRNA pathway effector protein, Ago-2, by the Domino ortholog p400. They add functional insights to previous observations of this protein’s antiviral and RNA interference regulatory activities in Drosophila melanogaster. IMPORTANCE Female Aedes aegypti mosquitoes are vectors of human-infecting arthropod-borne viruses (arboviruses). In recent decades, the incidence of arthropod-borne viral infections has grown dramatically. Vector competence is influenced by many factors, including the mosquito’s antiviral defenses. The exogenous small interfering RNA (siRNA) pathway is a major antiviral response restricting arboviruses in mosquitoes. While the roles of the effectors of this pathway, Argonaute-2 and Dicer-2 are well characterized, nothing is known about its regulation in mosquitoes. In this study, we demonstrate that A. aegypti p400, whose ortholog Domino in Drosophila melanogaster is a chromatin-remodeling ATPase member of the Tip60 complex, regulates siRNA pathway activity and controls ago-2 expression levels. In addition, we found p400 to have antiviral activity against different arboviruses. Therefore, our study provides new insights into the regulation of the antiviral response in A. aegypti mosquitoes.
Published: 2020
Full Text: View/download PDF

50. High Transmission Potential of West Nile Virus Lineage 1 for Cx. pipiens s.l. of Iran

Author: Xavier de Lamballerie, Hasan Bakhshi, Sedigheh Zakeri, Navid Dinparast-Djadid, Laurence Mousson, Anna-Bella Failloux, Marie Vazeille, Abbasali Raz, Malaria and Vector Research Group (MVRG), Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), The present article is a part of a project that has been supported through a grant from Institut Pasteur International Network (IPIN, MATI) to ABF and NDD. HB as a Ph.D. student of medical biotechnology also received scholarships from IPP, Campus France, and education office of IPI., DEMESLAY GOUGAM, MARIE, Institut Pasteur [Paris], and Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, Lineage (genetic), experimental infections, West Nile virus, viruses, 030231 tropical medicine, lcsh:QR1-502, Mosquito population, Biology, Iran, medicine.disease_cause, Arbovirus, lcsh:Microbiology, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Virology, High transmission, parasitic diseases, medicine, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, vector competence, virus diseases, medicine.disease, 3. Good health, 030104 developmental biology, Infectious Diseases, Transmission (mechanics), Vector (epidemiology), [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Viral genotype
Abstract: Vector competence is an important parameter in evaluating whether a species plays a role in transmission of an arbovirus. Although the protocols are similar, interpretation of results is unique given the specific interactions that exist between a mosquito population and a viral genotype. Here, we assessed the infection (IR), dissemination (DR), and transmission (TR) rates of Cx. pipiens s.l., collected from Iran, for West Nile virus (WNV) lineage 1a. We showed that Cx. pipiens s.l. mosquitoes in Iran were susceptible to WNV with IR up to 89.7%, 93.6%, and 83.9% at 7, 14, and 21 days post-infection (dpi) respectively. In addition, DR and TR reached respectively 92.3% and 75.0% at 21 dpi, and the number of viral particles delivered with saliva reached up to 1.33 &times, 105 particles. Therefore, an unexpected high risk of WNV dissemination in the region where Cx. pipiens s.l. mosquitoes are well established should be considered carefully and surveillance measures implemented accordingly.
Published: 2020
Full Text: View/download PDF

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Publication Type

Journal

Region

Database

Publisher

148 results on '"Arbovirus et Insectes Vecteurs"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources