1. Extraction, Purification, Structural Characterization, and Antitumor Effects of Water-Soluble Intracellular Polysaccharide (IPSW-1) from Phellinus igniarius Mycelia.
- Author
-
Boateng ID, Guo YZ, and Yang XM
- Subjects
- Humans, Hep G2 Cells, Polysaccharides chemistry, Polysaccharides pharmacology, Polysaccharides isolation & purification, Cell Movement drug effects, Fungal Polysaccharides chemistry, Fungal Polysaccharides pharmacology, Fungal Polysaccharides isolation & purification, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents isolation & purification, Basidiomycota chemistry, Mycelium chemistry, Cell Proliferation drug effects, Apoptosis drug effects
- Abstract
Phellinus igniarius is a commonly used Chinese medicine fungus, and its polysaccharide is a valuable bioactive with antioxidant, antiaging, antitumor activities, etc. However, their bioactivities are influenced by their structural and physicochemical properties. Hence, this research isolated and purified homogeneous water-soluble intracellular polysaccharide (IPSW-1) from P. igniarius mycelia. A coherent study of its structural characteristics, conformation, and antitumor mechanisms was evaluated. The results showed IPSW-1 has no triple helical conformation according to the Congo red test. Based on FT-IR, periodate oxidation, Smith degradation, methylation analysis,
1 H and13 C NMR spectroscopy data, and IPSW-1 consisted of α-d-glucopyranose (Glc p ). The backbone of IPSW-1 consisted primarily of repeating three (1 → 6)-linked α-d-Glc p and one (1 → 3,4)-linked α-d-Glc p , with one terminal α-d-Glc p as side chains of 3-O-connected to the main chain for every four residues. The IPSW-1 had an inhibitory influence on HepG2 cell proliferation and inhibited the migration and invasion ability by down-regulating the expression levels of MMP-7 and RhoA. Moreover, IPSW-1 could inhibit the lysis of autophagosomes to inhibit autophagy and regulate mitochondrial membrane potential and pro-apoptotic protein Bax, which causes the caspase cascade to promote apoptosis, thereby inhibiting the role of tumor cells. These findings show IPSW-1 holds potential as an innovative functional food.- Published
- 2024
- Full Text
- View/download PDF