1. Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats.
- Author
-
Sheng X, Nagaoka S, Hashimoto M, Amiya Y, Beppu M, Tsukamoto K, Yanase E, Tanaka M, and Matsui T
- Subjects
- Administration, Oral, Animals, Chromatography, Liquid methods, Male, Peptide Fragments pharmacokinetics, Peptides chemistry, Rats, Wistar, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Rats, Antigens, Plant administration & dosage, Antigens, Plant blood, Globulins administration & dosage, Peptide Fragments blood, Seed Storage Proteins administration & dosage, Seed Storage Proteins blood, Soybean Proteins administration & dosage, Soybean Proteins blood
- Abstract
In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after administration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography-time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75-756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF