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Detection of peanut allergen in human blood after consumption of peanuts is skewed by endogenous immunoglobulins.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2017 Jan; Vol. 440, pp. 52-57. Date of Electronic Publication: 2016 Nov 11. - Publication Year :
- 2017
-
Abstract
- Some studies have suggested that allergens may appear in the circulation after ingestion of allergenic food sources. The reported levels of allergen in serum, however, are low, and conclusions between studies differ. Here, we investigated factors that determine the detection of allergens in serum after consumption of peanuts. Ten healthy volunteers ingested 100g of light-roasted peanuts. Serum samples were taken at regular intervals for six hours. A double monoclonal sandwich ELISA was used to analyse the presence and quantity of the major peanut allergen Ara h 6 in serum. In 4 out of 10 subjects, no Ara h 6 could be detected. Purified Ara h 6 that was digested in vitro was still reactive in the ELISA, rejecting the possibility that digestion leads to small peptides that could not be detected. Spiking of purified Ara h 6 in baseline serum showed that the pre-ingestion serum of these four subjects partially prevented Ara h 6 to react in the ELISA, with a reduction of reactivity of up to 3 orders of magnitude or more. Pre-ingestion serum of the other six subjects did not show such an effect. The reduction of reactivity of Ara h 6 coincided with high titres of IgG and IgG4, and removal of IgG from pre-ingestion serum abolished this effect completely, indicating that IgG and IgG4 inhibited the reactivity of Ara h6 in the ELISA. We conclude that some individuals have IgG and IgG4 against food allergens in their blood, which interferes with detection of such food allergens in serum. Because this effect does not occur for each individual, the possibility of such interference should be taken into consideration when interpreting immunochemical studies on the absorption of food allergens in serum.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- 2S Albumins, Plant immunology
Antibodies, Monoclonal immunology
Antigens, Plant immunology
Arachis adverse effects
Biomarkers blood
Digestion
Epitopes
Female
Healthy Volunteers
Humans
Hydrolysis
Male
Peanut Hypersensitivity blood
Peanut Hypersensitivity immunology
Predictive Value of Tests
Reproducibility of Results
Time Factors
Young Adult
2S Albumins, Plant blood
Antigens, Plant blood
Arachis immunology
Enzyme-Linked Immunosorbent Assay methods
Immunoglobulin G blood
Peanut Hypersensitivity diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 440
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 27845035
- Full Text :
- https://doi.org/10.1016/j.jim.2016.11.002