1. Catecholaminergic regulation of proliferation and survival in rat forebrain paraventricular germinal cells
- Author
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Lukhbir S Gill, Fiona E. Holmes, Hannah Brennan, Laurence W. Haynes, Vijay K Pabbathi, Michel Vignes, Anne Muxworthy, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Plasticité cérébrale (PC), and Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Phenylephrine ,MESH: Radioligand Assay ,MESH: Dose-Response Relationship, Drug ,Phenylephrine ,Radioligand Assay ,MESH: Prosencephalon ,Catecholamines ,MESH: Pregnancy ,0302 clinical medicine ,Pregnancy ,MESH: Animals ,Axon ,Cells, Cultured ,Catecholaminergic ,0303 health sciences ,Neocortex ,Stem Cells ,MESH: Phenethylamines ,General Neuroscience ,MESH: Tetralones ,Immunohistochemistry ,MESH: Clonidine ,Cell biology ,Neuroepithelial cell ,medicine.anatomical_structure ,MESH: Cell Survival ,MESH: Cell Division ,Female ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Cell Division ,MESH: Cells, Cultured ,medicine.medical_specialty ,MESH: Rats ,Cell Survival ,MESH: Stem Cells ,In situ hybridization ,Biology ,Clonidine ,03 medical and health sciences ,Prosencephalon ,Internal medicine ,Phenethylamines ,MESH: Catecholamines ,medicine ,Animals ,Rats, Wistar ,Progenitor cell ,Molecular Biology ,Adrenergic alpha-Antagonists ,Tetralones ,030304 developmental biology ,Dose-Response Relationship, Drug ,MESH: Immunohistochemistry ,MESH: Rats, Wistar ,Rats ,Endocrinology ,Cell culture ,Forebrain ,MESH: Adrenergic alpha-Antagonists ,Neurology (clinical) ,MESH: Female ,MESH: Paraventricular Hypothalamic Nucleus ,030217 neurology & neurosurgery ,Paraventricular Hypothalamic Nucleus ,Developmental Biology - Abstract
International audience; We have investigated the possible role of alpha1-adrenoreceptors in regulating the germination of progenitor cells cultured from embryonic rat neocortex. High binding levels of the alpha1-selective radioligand 3[H]prazosin were detected in the forebrain of the rat embryo at E13, and the greatest density of binding sites was localized to the ventricular and subventricular zones. Catecholamine-containing axon terminals were present in these zones in the same period. Germinal neuroepithelial cells retained specific 3[H]prazosin binding in culture. Approximately 25% of cells in culture displayed complex intracellular Ca2+ transients in response to phenylephrine, many of which were abolished with the alpha1B antagonist, chloroethylclonidine. Cultures exhibited concentration-dependent catecholamine stimulation of DNA synthesis mediated by alpha1 receptors in serum-limited conditions. Neuroepithelial cells were labelled via their ventricular processes by intraventricular injection of Fast blue in E13 embryos prior to transfer of the neocortex to dissociated cell culture. Many of labelled cells were present in culture in germinal foci. Some cells which migrated from these foci underwent apoptosis, as determined by TUNEL in situ hybridization. During a transitory period of up to 48 h in culture, alpha1-adrenoreceptor activation by phenylephrine or noradrenaline increased the number of surviving cells. Apoptosis was observed in vivo in both ventricular and subventricular zones of the neocortex from E13 to E15 in increasing numbers. We propose that both the supply of noradrenaline to forebrain germinal cells, and the expression of alpha1-adrenoreceptors on their surface could act to determine whether they die or continue to proliferate.
- Published
- 1997