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Catecholaminergic regulation of proliferation and survival in rat forebrain paraventricular germinal cells
- Source :
- Brain Research, Brain Research, Elsevier, 1997, 760 (1-2), pp.22-33. ⟨10.1016/s0006-8993(97)00272-2⟩
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- International audience; We have investigated the possible role of alpha1-adrenoreceptors in regulating the germination of progenitor cells cultured from embryonic rat neocortex. High binding levels of the alpha1-selective radioligand 3[H]prazosin were detected in the forebrain of the rat embryo at E13, and the greatest density of binding sites was localized to the ventricular and subventricular zones. Catecholamine-containing axon terminals were present in these zones in the same period. Germinal neuroepithelial cells retained specific 3[H]prazosin binding in culture. Approximately 25% of cells in culture displayed complex intracellular Ca2+ transients in response to phenylephrine, many of which were abolished with the alpha1B antagonist, chloroethylclonidine. Cultures exhibited concentration-dependent catecholamine stimulation of DNA synthesis mediated by alpha1 receptors in serum-limited conditions. Neuroepithelial cells were labelled via their ventricular processes by intraventricular injection of Fast blue in E13 embryos prior to transfer of the neocortex to dissociated cell culture. Many of labelled cells were present in culture in germinal foci. Some cells which migrated from these foci underwent apoptosis, as determined by TUNEL in situ hybridization. During a transitory period of up to 48 h in culture, alpha1-adrenoreceptor activation by phenylephrine or noradrenaline increased the number of surviving cells. Apoptosis was observed in vivo in both ventricular and subventricular zones of the neocortex from E13 to E15 in increasing numbers. We propose that both the supply of noradrenaline to forebrain germinal cells, and the expression of alpha1-adrenoreceptors on their surface could act to determine whether they die or continue to proliferate.
- Subjects :
- MESH: Phenylephrine
MESH: Radioligand Assay
MESH: Dose-Response Relationship, Drug
Phenylephrine
Radioligand Assay
MESH: Prosencephalon
Catecholamines
MESH: Pregnancy
0302 clinical medicine
Pregnancy
MESH: Animals
Axon
Cells, Cultured
Catecholaminergic
0303 health sciences
Neocortex
Stem Cells
MESH: Phenethylamines
General Neuroscience
MESH: Tetralones
Immunohistochemistry
MESH: Clonidine
Cell biology
Neuroepithelial cell
medicine.anatomical_structure
MESH: Cell Survival
MESH: Cell Division
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Cell Division
MESH: Cells, Cultured
medicine.medical_specialty
MESH: Rats
Cell Survival
MESH: Stem Cells
In situ hybridization
Biology
Clonidine
03 medical and health sciences
Prosencephalon
Internal medicine
Phenethylamines
MESH: Catecholamines
medicine
Animals
Rats, Wistar
Progenitor cell
Molecular Biology
Adrenergic alpha-Antagonists
Tetralones
030304 developmental biology
Dose-Response Relationship, Drug
MESH: Immunohistochemistry
MESH: Rats, Wistar
Rats
Endocrinology
Cell culture
Forebrain
MESH: Adrenergic alpha-Antagonists
Neurology (clinical)
MESH: Female
MESH: Paraventricular Hypothalamic Nucleus
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 760
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....9f0ee86789b4ee662cb31a2418a587b7