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1. Bibliometric survey and network analysis of biomimetics and nature inspiration in engineering science

Author

Vincent Gerbaud, Hugues Leiser, Johnny Beaugrand, Bernard Cathala, Carole Molina-Jouve, Anne Marie Gue, Laboratoire de Génie Chimique (LGC), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole)

Subjects
[SDE.IE]Environmental Sciences/Environmental Engineering, engineering, Biophysics, nature inspiration, [CHIM.MATE]Chemical Sciences/Material chemistry, Biochemistry, Biomimetics, bioinspiration, Molecular Medicine, Prospective Studies, biomimetics, bibliometrics, Engineering (miscellaneous), Biotechnology
Abstract

The field encompassing biomimetics, bioinspiration and nature inspiration in engineering science is growing steadily, pushed by exogenous factors like the search for potentially sustainable engineering solutions that might already exist in nature. With the help of information provided by a bibliometric database and further processed with a dynamic network and semantic analysis tool, we provide insight at two scales into the corpus of nature-inspired engineering field and its dynamics. At the macroscale, the Web of Science® (WoS) categories, countries and institutions are ranked and ordered by thematic clusters and country networks, highlighting the leading countries and institutions and how they focus on specific topics. Such an insight provides an overview at the macroscale that can be valuable to orient scientific strategy at the country level. At the mesoscale, where science is incarnated by collaborative networks of authors and institutions that run across countries, we identify six semantic clusters and subclusters within them, and their dynamics. We also pinpoint leading academic collaborative networks and their activity in relation to the six semantic clusters. Trends and prospective are also discussed. Typically, one observes that the field is becoming mature since, starting by imitating nature, it proceeded with mimicking more complex natural structures and functions and now it investigates ways used in nature in response to changes in the environment and implements them in innovative and adaptive artefacts. The sophistication of devices, methods and tools has been increasing over the years as well as their functionalities and adaptability, whereas the size of devices has decreased at the same time.

Published
2022
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2. A Resazurin Based Bio Sensor for Detection of Organic Pollutants in Water

Author

Amani Migaou, Anne-Marie Gue, Marie-Charline Blatche, Daniel Esteve, and Ali Boukabache

Subjects
microsystem, organic pollutants detection, water analysis, General Works
Abstract

With the objective of water analysis, a microsystem was developed, by using Resazurin molecule as an indirect bio sensor. The detection principle consists to monitor optically the oxygen consumption of E. Coli bacteria towards their metabolism in presence of organic pollutants. Thus, aiming on Glucose detection, a concentration-dependent inhibition effect on oxygen consumption rate was evidenced in the [0–0.41 mM] range of organic pollutants.

Published
2017
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3. A LOW-COST AND EASY TO USE SORTING DEVICE FOR THE SEPARATION OF EXTRACELLULAR VESICLES FROM COMPLEX FLUIDS

Author

Lyne Pillemont, Daniel Guneysu, Wilfrid Boireau, Céline Elie-Caille, Anne-Marie Gue, PILLEMONT, Lyne, Une approche microfluidique générique pour la qualification des nanoparticules biologiques - - MADNESS2017 - ANR-17-CE09-0025 - AAPG2017 - VALID, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), ANR, Amy E. Herr, Joel Voldman, and ANR-17-CE09-0025,MADNESS,Une approche microfluidique générique pour la qualification des nanoparticules biologiques(2017)

Subjects
microlfuidic, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, microfluidic, [SPI.MECA.MEFL] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph], [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, cell sorting, extracellular vesicles, hydrodynamic filtration, [SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph], lab-on-chip, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract

International audience; Identifying and characterizing Extracellular vesicles (EVs) in biofluids could lead to unprecedented diagnosis/prognosis assays for a wide set of pathologies. Owe to their complexity in size, origin, membrane markers, there is currently no ideal technology available yet to sort, to quantify and characterize cell-derived microvesicles, and fully relate their structure and functions which have high potential in clinical diagnostics and early treatment of life threatening diseases such as cancer, cardio vascular diseases…. Lab-on-chip for EVs size-based separation Chip EVs capture Sample EVs characterization (AFM imaging, mass spectrometry) APPROACH We propose to couple a microfluidic device (LOC) enabling to separate particles from a complex sample to a device allowing the capture of particles on specific spots and their analysis. These methods allow obtaining important information on a chip about nanoscale vesicles in terms of size, membrane markers expression, and structure-function properties.

Published
2021

4. Holistic, Long-Term Management of People with Relapsing Multiple Sclerosis with Cladribine Tablets: Expert Opinion from France

Author

Jonathan Ciron, Bertrand Bourre, Giovanni Castelnovo, Anne Marie Guennoc, Jérôme De Sèze, Ali Frederic Ben-Amor, Carine Savarin, and Patrick Vermersch

Subjects
Multiple sclerosis, Cladribine tablets, Disease-modifying therapy, Holistic management, Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Cladribine tablets (CladT) has been available for therapeutic use in France since March 2021 for the management of highly active relapsing multiple sclerosis (RMS). This high-efficacy disease-modifying therapy (DMT) acts as an immune reconstitution therapy. In contrast to most high-efficacy DMTs, which act via continuous immunosuppression, two short courses of oral treatment with CladT at the beginning of years 1 and 2 of treatment provide long-term control of MS disease activity in responders to treatment, without the need for any further pharmacological treatment for several years. Although the labelling for CladT does not provide guidance beyond the initial treatment courses, real-world data on the therapeutic use of CladT from registries of previous clinical trial participants and patients treated in routine practice indicate that MS disease activity is controlled for a period of years beyond this time for a substantial proportion of patients. Moreover, this clinical experience has provided useful information on how to initiate and manage treatment with CladT. In this article we, a group of expert neurologists from France, provide recommendations on the initiation of CladT in DMT-naïve patients, how to switch from existing DMTs to CladT for patients with continuing MS disease activity, how to manage patients during the first 2 years of treatment and finally, how to manage patients with or without MS disease activity in years 3, 4 and beyond after initiating treatment with CladT. We believe that optimisation of the use of CladT beyond its initial courses of treatment will maximise the benefits of this treatment, especially early in the course of MS when suppression of focal inflammation in the CNS is a clinical priority to limit MS disease progression.

Published
2024
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5. Towards onchip EVs separation: a lab‐on‐chip approach. J Extracell Vesicles

Author

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne-Marie Gue, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Femto-st, MN2S

Subjects
[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph], [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.MAT] Engineering Sciences [physics]/Materials, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.MAT]Engineering Sciences [physics]/Materials
Abstract

International audience; Introduction: Owing to their complexity in size, origin, membrane markers, there is currently no ideal technology available to relate cell-derived microvesicles (EVs) structure and functions. All currently available methods (flow-cytometry, DLS, TRPS, etc.) have limits in their ability to capture the whole diversity of EVs populations and are not amenable to automation and large-scale analysis of numerous samples. In that context, the overall objective of this study is to develop a miniaturized platform allowing the isolation, fractionation and qualification of microvesicles in µL volume.Methods: Based on previous works (1), we propose a lab-on-chip coupling a hydrodynamic separation module enabling EVs separation according to their size to an affinity-trapping chamber compatible with subsequent SPR and AFM characterization. We designed and fabricated 2.5 × 2.5cm chips enabling the separation of vesicles at tunable cut-off (150-900nm). The proof-of-concept was done using fluorescent calibration particles (polystyrene and melanin resin nanoparticles) biofunctionalized with proteins and mimicking EVs in buffer solution.Results: Sample was introduced into the chip using a syringe pump or a pressure generator and the filtered sample was simply collected at the chip outlet and redirected towards a biodetection chamber designed as an array of gold plots functionalized with antibodies. We demonstrated the high quality separation of 490 nm nanoparticles from 920 nm particles in concentrated solution (2.109 to 2.1011 particles/µL). Following sorting step, biosynthetic particles were immunocaptured in a miniaturized module of the NBA platform (2, 3) for their subsequent analysis.Summary/Conclusion: We did the proof-of-concept of on-chip nanoparticles separation and capture demonstrating the ability of miniaturized systems to perform sample fractionation. The tunable properties of the device open the way to a versatile tool for pre-analytical steps of EVs, including sorting and concentration, even in complex media.

Published
2019

6. Towards on-chip EVs separation: a lab-on-chip approach

Author

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne-Marie Gue, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), International Society for Extracellular Vesicles, ANR-17-CE09-0025,MADNESS,Une approche microfluidique générique pour la qualification des nanoparticules biologiques(2017), Gue, Anne-Marie, Une approche microfluidique générique pour la qualification des nanoparticules biologiques - - MADNESS2017 - ANR-17-CE09-0025 - AAPG2017 - VALID, Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Lab ob Chip, Extracellular vesicles, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Exosomes
Abstract

International audience; Background and motivation Owe to their complexity in size, origin, membrane markers, there is currently no ideal technology available to relate cell-derived microvesicles (EVs) structure and functions. All currently available methods (flow-cytometry, DLS, TRPS, etc.) have limits in their ability to capture the whole diversity of EVs populations and are not amenable to automation and large-scale analysis of numerous samples. In that context, the overall objective of this study is to develop a miniaturized platform allowing the isolation, fractionation and qualification of microvesicles in µL volume. Methods Based on previous works (1), we propose a lab on chip coupling a hydrodynamic separation module enabling EVs separation according to their size to an affinity-trapping chamber compatible with subsequent SPR and AFM characterization. We designed and fabricated 2.5x2.5cm chips enabling the separation of vesicles at tunable cut-off (150-900nm). The proof-of-concept was done using fluorescent calibration particles (polystyrene and melanin resin nanoparticles) biofunctionnalized with proteins and mimicking EVs in buffer solution. Results Sample was introduced into the chip using a syringe pump or a pressure generator and the filtered sample was simply collected at the chip outlet and redirected towards a biodetection chamber designed as an array of gold plots functionalized with antibodies. We demonstrated the high quality separation of 490nm nanoparticles from 920nm particles in concentrated solution (2.109 to 2.1011 particles/µL). Following sorting step, biosynthetic particles were immunocaptured in a miniaturized module of the NBA platform (2,3) for their subsequent analysis. Conclusion We did the proof-of-concept of on-chip nanoparticles separation and capture demonstrating the ability of miniaturized systems to perform sample fractionation. The tunable properties of the device open the way to a versatile tool for preanalytical steps of EVs, including sorting and concentration, even in complex media.

Published
2019

7. Investigation of the selectivity of thrombin-binding aptamers for thrombin titration in murine plasma

Author

Ana Trapaidze, Jean-Pascal Herault, Anne Marie Gue, Jean-Marc Herbert, Aurélien Bancaud, Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), SANOFI Recherche, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), and Université Fédérale Toulouse Midi-Pyrénées

Subjects
0301 basic medicine, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Aptamer, Biomedical Engineering, Biophysics, Serum albumin, Biosensing Techniques, Plasma protein binding, 030204 cardiovascular system & hematology, Mice, Plasma, 03 medical and health sciences, 0302 clinical medicine, Thrombin, Aptamer assay, Electrochemistry, medicine, Animals, Humans, Binding site, Surface plasmon resonance, Serum Albumin, Binding Sites, biology, Chemistry, Aptasensor, Aptamer selectivity, Thrombosis, General Medicine, Aptamers, Nucleotide, Surface Plasmon Resonance, Molecular biology, Blood proteins, 3. Good health, 030104 developmental biology, biology.protein, Protein Binding, circulatory and respiratory physiology, Biotechnology, medicine.drug, Discovery and development of direct thrombin inhibitors
Abstract

International audience; Detection of thrombin in plasma raises timely challenges to enable therapeutic management of thrombosis in patients under vital threat. Thrombin binding aptamers represent promising candidates as sensing elements for the development of real-time thrombin biosensors; however implementation of such biosensor requires the clear understanding of thrombin-aptamer interaction properties in real-like environment. In this study, we used Surface Plasmon Resonance technique to answer the questions of specificity and sensitivity of thrombin detection by the thrombin-binding aptamers HD1, NU172 and HD22. We systematically characterized their properties in the presence of thrombin, as well as interfering molecular species such as the thrombin precursor prothrombin, thrombin in complex with some of its natural inhibitors, nonspecific serum proteins, and diluted plasma. Kinetic experiments show the multiple binding modes of HD1 and NU172, which both interact with multiple sites of thrombin with low nanomolar affinities and show little specificity of interaction for prothrombin vs. thrombin. HD22, on the other hand, binds specifically to thrombin exosite II and has no affinity to prothrombin at all. While thrombin in complex with some of its inhibitors could not be recognized by any aptamer, the binding of HD1 and NU172 properties is compromised by thrombin inhibitors alone, as well as with serum albumin. Finally, the complex nature of plasma was overwhelming for HD1, but we define conditions for the thrombin detection at 10nM range in 100-fold diluted plasma by HD22. Consequently HD22 showed key advantage over HD1 and NU172, and appears as the only alternative to design an aptasensor.

Published
2016
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8. A microsystem approach to measure the oxygen consumption of bacteria. Towards a precise evaluation of the BOD (Biological Oxygen Demand) parameter of wastewater

Author

Loic Recoules, Sulivan Jouanneau, Gerald Thouand, Anne-Marie Gue, Ali Boukabache, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Mines Nantes (Mines Nantes)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS), ANR-10-ECOT-0015,BIOGUARD(2010), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Mines Nantes (Mines Nantes)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDE.IE]Environmental Sciences/Environmental Engineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Abstract

International audience; The quantity of organic pollutants present in wastewater is classically evaluated by measuring the quantity of dissolved oxygen during five days; it is quantified by the so called BOD5 parameter (Biological Oxygen Demand) [1]. This work constitutes the first step of an overall strategy targeting to improve the monitoring of BOD5. We focus on the development of a microsystem approach allowing monitoring the O2 consumption induced by the biodegradation process of organic matter. To evaluate the organic pollutants concentration, we use Escherichia coli as bacterial indicator, confined in a PDMS-glass chip. Their metabolic activity in presence of organic molecules is deduced from their oxygen consumption. These measurements are ensured by optical sensors present in each of the five instrumented chambers of the chip. The results show that the microsystem approach is suitable to measure simultaneously different concentrations of organic load, and that it is possible to reduce the analysis time. By examining the O 2 diffusion towards the walls of the device, we analyse the different part of the experimental results; it allows predicting, in the future, a precise evaluation of the BOD value within a few hours.

Published
2018

9. Multilevel (3D) microfluidic technology for an innovative magnetic cell separation and couting platform

Author

Marc Fouet, Sébastien Cargou, Rémi Courson, Marie-Charline Blatché, Armelle Montrose, Karine Reybier, Anne-Marie Gue, Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3)

Subjects
Planar coils, Cell tagging, Multilayer (3D), [SDV]Life Sciences [q-bio], [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Magnetic separation, [SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph]
Abstract

International audience; We demonstrate a new concept of devices, which by combining 3D fluid engineering and localized mag-netic actuation enables the full integration of a cell tagging and magnetic separation device. We used a low cost, commercially available dry film (EMS Inc, Ohio, USA) that fits microfluidic requirements and gives the possibility to build easily 3D microfluidic structures. The labelling of blood monocytes with su-perparamagnetic particles was performed "up stream" with the aim of a microparticles 3D micromixer while cell separation was achieved "downstream" through continuous vertical separation over integrated micro-coils.

Published
2014

10. AN INTEGRATED MAGNETIC PLANAR ACTUATOR REDEFINING MULTILEVEL (3D) MICROFLUIDIC STRATEGIES

Author

Sébastien Cargou, Marc Fouet, Armelle Montrose, David Bourrier, Karine Reybier, Anne-Marie Gue, Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)

Subjects
LOC, "microfluidics", microfluidics, "cell sorting", "LOC", [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, cell sorting
Abstract

Accepté 10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, 10-14 June 2014 Dresden, Germany; International audience; Super-paramagnetic micro particles (SMPs) are broadly used in medical and biological applications (from cellular to genomic scales), but the handling tools for those particles remain mostly non-integrated. Since a decade, a few solutions for integrated actuation have been proposed and paved the way to the micro-scale control of SMPs. So far, spiral planar coils showed the best compromises between the available magnetic force and the actuator footprint. Albeit the technology to integrate this type of coils with microfluidic structures exists, it is hard to find examples of dual architectures using those two aspects for concrete applications. We therefore developed a SU-8 dry film lamination technique, which can be used with standard micro technology processes. It allows us to manufacture lab on chip integrating miniaturized electromagnets. Using that system, we achieved to separate a solution of SMPs with an 80% efficiency at a 2.5 µL/min flow rate (4.6 mm/s). It is also possible to perform trapping (all the beads stay on the coils) at lower flow rate.

Published
2014

11. Genetic landscape of a large cohort of Primary Ovarian Insufficiency: New genes and pathways and implications for personalized medicine

Author

Abdelkader Heddar, Cagri Ogur, Sabrina Da Costa, Inès Braham, Line Billaud-Rist, Necati Findlinki, Claire Beneteau, Rachel Reynaud, Khaled Mahmoud, Stéphanie Legrand, Maud Marchand, Isabelle Cedrin-Durnerin, Adèle Cantalloube, Maeliss Peigne, Marion Bretault, Benedicte Dagher-Hayeck, Sandrine Perol, Celine Droumaguet, Sabri Cavkaytar, Carole Nicolas-Bonne, Hanen Elloumi, Mohamed Khrouf, Charlotte Rougier-LeMasle, Melanie Fradin, Elsa Le Boette, Perrine Luigi, Anne-Marie Guerrot, Emmanuelle Ginglinger, Amandine Zampa, Anais Fauconnier, Nathalie Auger, Françoise Paris, Elise Brischoux-Boucher, Christelle Cabrol, Aurore Brun, Laura Guyon, Melanie Berard, Axelle Riviere, Nicolas Gruchy, Sylvie Odent, Brigitte Gilbert-Dussardier, Bertrand Isidor, Juliette Piard, Laetitia Lambert, Samir Hamamah, Anne Marie Guedj, Aude Brac de la Perriere, Hervé Fernandez, Marie-Laure Raffin-Sanson, Michel Polak, Hélène Letur, Sylvie Epelboin, Genevieve Plu-Bureau, Sławomir Wołczyński, Sylvie Hieronimus, Kristiina Aittomaki, Sophie Catteau-Jonard, and Micheline Misrahi

Subjects
Primary ovarian insufficiency, Personalized medicine, NF-KB, Post - translational regulation, Meiosis/DNA repair genes, Mitophagy, Medicine, Medicine (General), R5-920
Abstract

Summary: Background: Primary Ovarian Insufficiency (POI), a public health problem, affects 1-3.7% of women under 40 yielding infertility and a shorter lifespan. Most causes are unknown. Recently, genetic causes were identified, mostly in single families. We studied an unprecedented large cohort of POI to unravel its molecular pathophysiology. Methods: 375 patients with 70 families were studied using targeted (88 genes) or whole exome sequencing with pathogenic/likely-pathogenic variant selection. Mitomycin-induced chromosome breakages were studied in patients’ lymphocytes if necessary. Findings: A high-yield of 29.3% supports a clinical genetic diagnosis of POI. In addition, we found strong evidence of pathogenicity for nine genes not previously related to a Mendelian phenotype or POI: ELAVL2, NLRP11, CENPE, SPATA33, CCDC150, CCDC185, including DNA repair genes: C17orf53(HROB), HELQ, SWI5 yielding high chromosomal fragility. We confirmed the causal role of BRCA2, FANCM, BNC1, ERCC6, MSH4, BMPR1A, BMPR1B, BMPR2, ESR2, CAV1, SPIDR, RCBTB1 and ATG7 previously reported in isolated patients/families. In 8.5% of cases, POI is the only symptom of a multi-organ genetic disease. New pathways were identified: NF-kB, post-translational regulation, and mitophagy (mitochondrial autophagy), providing future therapeutic targets. Three new genes have been shown to affect the age of natural menopause supporting a genetic link. Interpretation: We have developed high-performance genetic diagnostic of POI, dissecting the molecular pathogenesis of POI and enabling personalized medicine to i) prevent/cure comorbidities for tumour/cancer susceptibility genes that could affect life-expectancy (37.4% of cases), or for genetically-revealed syndromic POI (8.5% of cases), ii) predict residual ovarian reserve (60.5% of cases). Genetic diagnosis could help to identify patients who may benefit from the promising in vitro activation-IVA technique in the near future, greatly improving its success in treating infertility. Funding: Université Paris Saclay, Agence Nationale de Biomédecine.

Published
2022
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12. Nanobubbles and gas dynamics during capillary filling of nanochannels

Author

Fabien Chauvet, Sandrine Geoffroy, Abdelkrim Hamouni, Marc Prat, Anne-Marie Gue, Pierre Joseph, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Laboratoire de Génie Chimique (LGC), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Laboratoire Matériaux et Durabilité des constructions (LMDC), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Institut de mécanique des fluides de Toulouse (IMFT), Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), and Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole)

Subjects
Physics::Fluid Dynamics, Nanochannels, Mécanique des fluides, Nanobubbles, Nanofluidics, Capillary filling, [PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph]
Abstract

International audience; This paper focuses on capillary filling at the nanoscale where deviations to the Washburn’s classical theory are observed. Imbibition experiments in microfabricated silicon-glass nanochannels with low aspect ratio (width >> depth and depths going from 400 nm down to 20 nm) are performed for several liquids. In all cases, as predicted by the Washburn’s law, liquid invasion front location evolves as the square root of time. However, filling kinetics slowdown compared to the Washburn’s law is measured in nanochannels for depths below ~ 100 nm. Furthermore, below a liquid-dependent depth threshold, we observe spontaneous bubbles formation behind the advancing meniscus. Bubbles dynamics (formation conditions and lifetime) are analyzed thanks to our experimental data involving several liquids and nanochannels depths. Viscous resistance induced by the bubbles presence is estimated using an effective medium approach. Conjointly, gas flow ahead of the advancing meniscus is modeled considering the gas as viscous and compressible. Influence of these effects on the filling kinetics is discussed.

Published
2012

13. Etude de filtres millimétriques accordables en technologie microfluidique

Author

Daouda Lamine Diedhiou, Stéphane Pinon, Eric Rius, Cédric Quendo, Jean-François Favennec, Benjamin Potelon, Ali Boukabache, Anne-Marie Gue, Norbert Fabre, Gaëtan Prigent, Véronique Conédéra, Jean-Yves Fourniols, Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (UMR 3192) (Lab-STICC), Université européenne de Bretagne - European University of Brittany (UEB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Lab-STICC_UBO_MOM_DIM, Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure d'Electrotechnique, d'Electronique, d'Informatique, d'Hydraulique et de Télécommunications (ENSEEIHT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
[SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SPI.TRON]Engineering Sciences [physics]/Electronics
Abstract

National audience; Cet article présente les travaux initiés au Lab-STICC et au LAAS sur la conception de dispositifs passifs hyperfréquences accordables sur la basse d'une approche microfluidique. L'idée est d'utiliser des micros canaux dans lesquels circulent des fluides. Ces fluides, diélectriques dans un premier temps, sont utilisés pour modifier la permittivité effective d'un substrat sur lequel est réalisée une structure planaire. La perturbation apportée par le fluide diélectrique va ainsi modifier la fréquence de travail du dispositif. En première approche, des stubs quart d'onde et des filtres passe-bande à stubs sont examinés.

Published
2011

14. Trapping Biological Species in a Lab-on-Chip Microsystem: Micro Inductor Optimization Design and SU8 Process

Author

Aurélien Bancaud, Rémy Fulcrand, Ali Boukabache, Christophe Escriba, Anne Marie Gue, David Jugieu, Philippe Artillan, Jean-Yves Fourniols, Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Christian Piguet, Ricardo Reis, Dimitrios Soudris, Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), and Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole)

Subjects
Finite element method, 02 engineering and technology, Inductor, 01 natural sciences, Analytical model, law.invention, Software, law, Hardware_GENERAL, Microsystem, 0103 physical sciences, Electronic engineering, [INFO.INFO-DL]Computer Science [cs]/Digital Libraries [cs.DL], [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 010302 applied physics, Physics, business.industry, Process (computing), Lab-on-a-chip, 021001 nanoscience & nanotechnology, magnetic bead separation, Computer Science::Other, Magnetic actuators, Magnetic field, Biological species, Fluidic channel, 0210 nano-technology, business, force
Abstract

International audience; Micro-spiral inductor dedicated to microbeads manipulation in a fluidic channel had been optimized by analytical modelling correlated to multi physics fem numerical Maxwell®3D L software. Main advantage of the analytical model described below is time analysis calculus decrease of and the capability offered to optimize geometrical and electrical parameters of the inductor. First experimental results show a good correlation between simulation and realized integrating micro-devices in a fluidic channel.

Published
2008
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15. Nanoscale actuation of electrokinetic flows on thermoreversible surfaces

Author

Jan Sudor, Alain Estève, Mehdi Djafari-Rouhani, Anne Marie Gue, Yves J. Chabal, Guillaume Paumier, Meng Li, and Francoise Vinet

Subjects
chemistry.chemical_classification, Materials science, Polarity (physics), Clinical Biochemistry, Acrylic Resins, Nanotechnology, Polymer, Dynamic control, Microfluidic Analytical Techniques, Biochemistry, Analytical Chemistry, Coupling (electronics), Electrokinetic phenomena, Kinetics, chemistry, Thermal, Spectroscopy, Fourier Transform Infrared, Electrochemistry, Composite material, Electroosmosis, Actuator, Nanoscopic scale
Abstract

We report on a novel approach for controlling nanohydrodynamic properties at the solid-liquid interfaces through the use of stimuli-responding polymer coatings. The end-tethered polymers undergo a phase separation upon external activation. The reversible change in the thickness and polarity of the grafted polymers yields in a dynamic control of the surface-generated, electrokinetic phenomena. Nonactivated, swollen polymers are thicker than the electrical double layer (EDL) and prohibit the development of an EOF even on charged surfaces. On the other hand, activated polymer chains shrink and become thinner than the EDL and allow for the EOF to build up unimpeded. We show here that, for given experimental conditions, the EOF velocity on the shrunken surface is 35 times greater than the one on the nonactivated surface. Furthermore, we reveal that coupling of such surfaces with dense arrays of thermal actuators developed in our laboratory can lead to novel micro- and nanofluidic devices.

Published
2008

16. Nanobiosensors based on silicon nanowires

Author

Eude, L., Pribat, D., Charlot, S., Anne-Marie Gue, Laurent Mugherli, Michel Fromant, Pierre Plateau, Sylvain Blanquet, Lehoucq, G., Bondavalli, P., Legagneux, P., Plateau, Pierre, Laboratoire de Biochimie de l'Ecole polytechnique (BIOC), and École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2007

17. A novel and simplified procedure for patterning hydrophobic and hydrophilic SAMs for microfluidic devices by using UV photolithography

Author

Jan Sudor, N. Jaffrezic, Eric Besson, Jacques Tardy, Hafsa Korri-Youssoufi, Anne-Marie Gue, Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Centre de génie électrique de Lyon (CEGELY), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-École Centrale de Lyon (ECL), Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
SURFACE, Infrared spectroscopy, GLASS, 02 engineering and technology, Sulfonic acid, 010402 general chemistry, 01 natural sciences, OLIGONUCLEOTIDES, Contact angle, SELF-ASSEMBLED MONOLAYERS, PROTECTING GROUPS, X-ray photoelectron spectroscopy, CHEMISTRY, Monolayer, Electrochemistry, Organic chemistry, General Materials Science, TOTAL ANALYSIS SYSTEMS, Spectroscopy, chemistry.chemical_classification, [SPI.NRJ]Engineering Sciences [physics]/Electric power, Substrate (chemistry), Self-assembled monolayer, Surfaces and Interfaces, ORGANIC-SYNTHESIS, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, POLYMERIZATION, chemistry, Chemical engineering, Self-assembly, 0210 nano-technology, CELL-ADHESION
Abstract

International audience; This work describes how selective patterning of hydrophobic and hydrophilic areas inside microchannels of microfluidic devices can be achieved by combining well-known chemical protocols and standard photolithography equipment (365 nm). Two techniques have been performed and compared. The first technique is based on the preparation of self-assembled monolayers of photocleavable organosilane and the second one on photoassisted grafting (365 nm) of self-assembled monolayers (SAMs) on a silicon or glass substrate. In the first case, we begin with monolayers carrying an o-nitrobenzyl function (hydrophobic area) that is photochemically cleaved, revealing a carboxylic acid group (hydrophilic area). The problem is that the energy necessary to cleave this monolayer is too high and the reaction time is more than 1 h with 50 mW/cm(2) irradiation flux. To overcome this practical disadvantage, we propose another approach that is based on the thiol-ene reaction with benzophenone as photoinitiator. In this approach, a monolayer of mercaptopropyltrimethoxysilane (MPTS) is prepared first. Subsequently, a hydrocarbon chain is photografted locally onto the thiol layer, forming a hydrophobic surface while the reminding unmodified thiol surface is oxidized into sulfonic acid (hydrophilic area). We demonstrated the feasibility of this approach and synthesized high-quality self-assembled monolayers by UV grafting with an irradiation time of 30 s at 365 nm (50 mW/cm(2)). The modified surfaces have been characterized by contact angle measurements, X-ray photoelectron spectroscopy (XPS), AFM, and multiple internal reflection infrared spectroscopy (MIR-FTIR). The difference in the contact angles on the hydrophilic and hydrophobic surfaces reached a remarkable 77 degrees. We have also demonstrated that this method is compatible with selective surface grafting inside microfluidic channels.

Published
2006
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18. Effets de l'interface électrocinétique dans les micro-canaux

Author

Tardu, Sedat F., Colin, S., Henri Camon, Anne-Marie Gue, Laboratoire des Écoulements Géophysiques et Industriels [Grenoble] (LEGI), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)

Subjects
[PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph], [SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph]
Published
2003

19. Yucatec Maya language planning and bilingual education in the Yucatan

Author

Anne Marie Guerrettaz, Eric J. Johnson, and Gisela Ernst-Slavit

Subjects
normalización lingüística, normativización lingüística, educación bilingüe, maya yucateco, méxico, maestros de lenguas originarias, yucatán, la política del lenguaje, etnografía, interacción en el salón de clase, Education
Abstract

The rapid decline of indigenous languages represents one of the most troubling topics within applied linguistics. Teachers’ implementation of indigenous language planning through their pedagogical practices is a significant but under-researched issue. This ethnographic study examines a Maya language program (i.e., professional development) for 1,600 teachers in the Yucatan’s Intercultural Bilingual Education (EIB) system, and K-12 schools in Maya-speaking communities where they worked. Using longitudinal data (2010-2016), analysis centered on the creation and promulgation of the Norms of Writing for the Maya Language (2014) and related language policy. Findings illustrate: 1) the importance of increasing the quantity of Maya-speaking teachers, and 2) a clash between widespread orthographic variation in Maya and teachers’ standard language-culture. The new standard has not been implemented in EIB, which still does not in practice require Maya proficiency of teachers. This research discusses possible benefits and risks of a standard Maya for EIB.

Published
2020
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20. Low cost integration of multilevel lab-on-a-chip using a new generation of dry film photoresists

Author

Rémi Courson, Sébastien Cargou, Véronique Conédéra, Marc Fouet, Anne-Marie Gue, Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), and Université Fédérale Toulouse Midi-Pyrénées

Subjects
[CHIM.POLY]Chemical Sciences/Polymers, [CHIM.MATE]Chemical Sciences/Material chemistry, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [CHIM.INOR]Chemical Sciences/Inorganic chemistry
Abstract

International audience; We present the use of a novel dry film photoresist DF-1050 (EMS) for the integration of multilevel microfluidic systems. We showed that high resolution and high aspect ratio (7:1) can be achieved for free standing structures. We proved also that the dry film autofluorescence level is compatible with most of lab-on-a-chip applications and established the biocompatibility of this dry film. Ultimately, we demonstrated that this low-cost material combined with multilevel lamination technology would allow introducing massively 3D microfluidic in lab-on-a-chip devices.

21. An easy surface modification in heterogeneous nano- and micro- fluidic devices

Author

Thi Thu Vu, Marc Fouet, Anne-Marie Gue, Jan Sudor, AMSN Department, University of Science and Technology of Hanoi (USTH), Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), University of sciences and technologies of hanoi (USTH), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)

Subjects
[SDV]Life Sciences [q-bio], [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

22. A MICROFLUIDIC MAGNETIC HYBRID ACTUATOR FOR ADVANCED HANDLING FUNCTIONS AT CELL RESOLUTION

Author

Marc Fouet, Rémi Courson, Xavier Dollat, Daniel Medale, Anne-Marie Gue, Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Service Techniques et Équipements Appliqués à la Microélectronique (LAAS-TEAM), Service Instrumentation Conception Caractérisation (LAAS-I2C), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), and Université de Toulouse (UT)

Subjects
LOC, "microfluidics", microfluidics, "magnetic cell sorting", "LOC", [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, magnetic cell sorting
Abstract

Accepté 10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers 10-14 june 2014 Dresden Germany; International audience; In microfluidics, the use of super-paramagnetic microparticles (SMPs) has experienced a dramatic increase over the last decade, but control of the magnetic field at micro scale is still a topical issue. Permanent magnets don't offer a programmable approach, external coils require a certain power, and neither are usually integrated. Hybrid systems, which include external magnets to generate a high and homogeneous magnetic field, and integrated coils that produce a high gradient, are seemingly a promising way to reach a higher precision in magnetic actuation, not mentioning the forces they create on SMPs can be attractive or repulsive. The goal of our work, for one part, is to develop a simple solution for the micro-fabrication of this kind of devices, but also to delve into the design possibilities, for instance to create a separation stage or investigate more exotic functions like focusing (without leading to a dilution or an increase in the flow rate, unlike most hydrodynamic focusing techniques).

23. Mise au point d'un micro-système à détection de fluorescence induite par laser pour l'analyse de routine de l'homocystéine

Author

Causse, E., Suzanne Fery-Forgues, Anne-Marie Gue, Fernanda Rodrigues, François Couderc, Interactions moléculaires et réactivité chimique et photochimique (IMRCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Fédération de Recherche Fluides, Energie, Réacteurs, Matériaux et Transferts (FERMAT), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
stomatognathic diseases, InformationSystems_MODELSANDPRINCIPLES, ComputingMilieux_THECOMPUTINGPROFESSION, GeneralLiterature_INTRODUCTORYANDSURVEY, ComputingMilieux_COMPUTERSANDEDUCATION, [CHIM.OTHE]Chemical Sciences/Other
Abstract

communication orale

24. Nanobiosensors based on functionalised silicon nanowires

Author

Lehoucq, G., Laurent Mugherli, Baraton, L., Michel Fromant, Gowtham, M., Bondavalli, P., Pierre Plateau, Legagneux, P., Sylvain Blanquet, Charlot, S., Anne-Marie Gue, Pribat, D., Laboratoire de Biochimie de l'Ecole polytechnique (BIOC), and École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]

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