1. Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits
- Author
-
Sabine Vierecke, Kay Foetisch, Andreas Reuter, Anna H. Malczyk, Annette Jamin, Jonas Lidholm, Stefan Vieths, Stefan Schülke, Vera Mahler, Detlef Bartel, Stephan Scheurer, and Andrea Wangorsch
- Subjects
Allergy ,Molecular Sequence Data ,Cross Reactions ,Biology ,Immunoglobulin E ,medicine.disease_cause ,law.invention ,Pathogenesis ,Allergen ,Solanum lycopersicum ,law ,medicine ,Humans ,Potency ,Amino Acid Sequence ,Cloning, Molecular ,Base Sequence ,Rhinitis, Allergic, Seasonal ,food and beverages ,Antigens, Plant ,medicine.disease ,Recombinant Proteins ,In vitro ,Fruit ,Immunology ,Recombinant DNA ,biology.protein ,Food Hypersensitivity ,Food Science ,Biotechnology ,Fruit allergy - Abstract
Scope The aim was to investigate the potential contribution of a major birch pollen Bet v 1-homologous allergen to birch pollen-associated tomato fruit allergy. Methods and results Two isoforms of a Bet v 1-homologous protein (designated Sola l 4) from tomato fruit were identified by cDNA-cloning and produced as recombinant proteins. Allergen-specific IgE levels to tomato, birch pollen, Bet v 1, and Sola l 4 were determined in birch pollen allergic patients with allergy or tolerance to tomato. Sola l 4 was recognized in 76% of birch/tomato allergic patients, while tomato- and Bet v 1-specific IgE was detectable in 64% and 81% of sera. Almost all patients sensitized to Bet v 1 reacted with Sola l 4. Both Sola l 4 isoforms displayed allergenic potency and IgE-cross-reactivity with Bet v 1 as investigated by competitive ELISA and in vitro mediator release assay. Nevertheless, the reactivity pattern of patients' sera was diverse. Conclusion Sola l 4, a novel pathogenesis related-10 protein, qualifies as major allergen in tomato fruits. Data suggest Sola l 4 as class II allergen. IgE-testing using Sola l 4 showed low clinical specificity, but high sensitivity in tomato allergic patients and will further improve component-resolved allergy diagnosis.
- Published
- 2014