80 results on '"Ametrano G"'
Search Results
2. Setting up an Italian end-to-end dosimetric audit for IMRT and VMAT: the experience of the OPRORA project
- Author
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De Angelis, C., primary, Della Monaca, S., additional, Fattibene, P., additional, Anello, P., additional, Quattrini, M.C., additional, Santavenere, F., additional, Soriani, A., additional, Sanguineti, G., additional, Pimpinella, M., additional, De Coste, V., additional, Arrichiello, C., additional, Ametrano, G., additional, Muto, P., additional, Bresciani, S., additional, Miranti, A., additional, Strigari, L., additional, Strolin, S., additional, and Morganti, A., additional
- Published
- 2021
- Full Text
- View/download PDF
3. Thermoluminescent dosimeters (TLDs-100) calibration for dose verification in photon and proton radiation therapy
- Author
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D'Avino V., Caruso M., Arrichiello C., Ametrano G., la Verde G., Muto P., Scifoni E., Tommasino F., Pugliese M., D'Avino, V., Caruso, M., Arrichiello, C., Ametrano, G., la Verde, G., Muto, P., Scifoni, E., Tommasino, F., and Pugliese, M.
- Abstract
Thermoluminescent dosimeters (TLDs) are practical, accurate, and precise tools for point dosimetry in medical physics applications. TLDs are nowadays extensively used to measure dose in conformal radiation therapy in order to guarantee the safety of the treatment. Several national and international organizations recommend checking the effective dose delivered to an individual patient by means of in vivo dosimetry. Modern radiotherapy techniques employing both photon and ion beams exhibit excellent target conformation throught high steep-dose gradients between tumour and adjacent organs and tissues. In this context, catching potential dose errors and uncertainties in treatment delivering is the first step to ensure the optimization of the treatment plan. This study shows the results of the characterization of TLDs-100 at two Italian facilities devoted to advanced radiation treatments with photon and proton therapy. The individual sensitivity factor was determined, and the calibration curves were carried out in the dose range 0–20 Gy. By the analysis of the dose response curves, the linear region was identified under the dose level of 10 Gy. Characterization of the TLDs-100 has enabled their use for in vivo dosimetry especially in the dose range corresponding to the linear region of the dose response curves.
- Published
- 2020
4. Preliminary investigation of performance of thermoluminescent dosimeters for dose verification in brachytherapy
- Author
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D'Avino V., Scarica M., Ametrano G., la Verde G., Manti L., Muto P., Pugliese M., Arrichiello C., D'Avino, V., Scarica, M., Ametrano, G., la Verde, G., Manti, L., Muto, P., Pugliese, M., and Arrichiello, C.
- Abstract
Brachytherapy represents the treatment of choice for many cancerous lesions, including skin tumours. Purpose of the study was to investigate the performance of thermoluminescent dosimeters of type 100, (TLD-100), for dose verification in high dose-rate treatment with a radioactive source of iridium-192. A set of TLDs-100 was calibrated with a 250 kVp X-ray beam in the dose range 0–5 Gy. Through a head and neck phantom, TLDs were fixed on districts corresponding to volumes of interest, including the target volume and some critical structures, then a single fraction of brachytherapy treatment was delivered. The dose measurements provided by TLDs were compared with the planned ones and the results were discussed in the light of limitations affecting the current treatment planning systems and critical aspects relating to brachytherapy implementation that still occurred in clinical settings. The findings of the study allow us to conclude that TLDs-100 show a good performance in the radiation field investigated and the use of so small, cheap and practical dosimetry system is potentially an optimal strategy of improving and standardizing the quality assurance protocol in brachytherapy.
- Published
- 2020
5. PRELIMINARY COMPARISON BETWEEN AUTOMATED AND MANUALLY PLANNED TREATMENTS FOR BRAIN METASTASIS
- Author
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Serra, M., Savino, F., Ametrano, G., Quarto, M., Tafuri, D., Loffredo, F., Muto, P., Serra, Marcello, Savino, Federica, Ametrano, Gianluca, Quarto, Maria, Tafuri, Domenico, Loffredo, Filomena, and Muto, Paolo
- Subjects
VMAT, Autoplanning, Brain metastasis, WBRT, OARs sparing, PlanIQ - Abstract
Introduction: Radiotherapy is increasingly used for the treatment of cancer patients and more and more personalized treatments are being developed to improve the patient’s quality of life. Today, the medical community is moving toward VMAT plans, which allow to deliver the prescription dose to the tumor and to spare surrounding healthy tissues. In this context the development of autoplanning system is desirable. Method and results: In this work we report the implementation, in the TPS RayStation, of the autoplanning algorithm that creates high quality plans, when brain metastases are involved. Conclusion: At the state of the art, the AP codes can be intended to lighten the work of the medical physicist so that he can refine the plan previously elaborated in AP modality or devote himself to more delicate situations.
- Published
- 2020
6. Effect of autoclaving on the surfaces of TiN-coated and conventional nickel–titanium rotary instruments
- Author
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Spagnuolo, G., Ametrano, G., DʼAntò, V., Rengo, C., Simeone, M., Riccitiello, F., and Amato, M.
- Published
- 2012
- Full Text
- View/download PDF
7. Effect of sterilization on the surface of rotary NiTi instruments evaluated by SEM and EDS: R72
- Author
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Simeone, M., Ametrano, G., DʼAntò, V., Rengo, C., Amato, M., and Spagnuolo, G.
- Published
- 2011
8. Analysis of mandibular first molars by micro-computed tomography (μCT): R2
- Author
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Riccitiello, F., Formisano, A., Ametrano, G., Simeone, M., Spagnuolo, G., and Rengo, S.
- Published
- 2011
9. Effects of sodium hypochlorite and ethylenediaminetetraacetic acid on rotary nickel-titanium instruments evaluated using atomic force microscopy
- Author
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Ametrano, G., DʼAntò, V., Di Caprio, M. P., Simeone, M., Rengo, S., and Spagnuolo, G.
- Published
- 2011
- Full Text
- View/download PDF
10. EP-1344 Helical Tomotherapy based SBRT in early stage lung cancer: a mono-institutional study (20142018)
- Author
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Pastore, F., primary, Rese, A., additional, Francomacaro, F., additional, Cammarota, F., additional, Ametrano, G., additional, Toledo, D., additional, and Iorio, V., additional
- Published
- 2019
- Full Text
- View/download PDF
11. P203 - Setting up an Italian end-to-end dosimetric audit for IMRT and VMAT: the experience of the OPRORA project
- Author
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De Angelis, C., Della Monaca, S., Fattibene, P., Anello, P., Quattrini, M.C., Santavenere, F., Soriani, A., Sanguineti, G., Pimpinella, M., De Coste, V., Arrichiello, C., Ametrano, G., Muto, P., Bresciani, S., Miranti, A., Strigari, L., Strolin, S., and Morganti, A.
- Published
- 2021
- Full Text
- View/download PDF
12. Correction: Metabolic syndrome, endocrine disruptors and prostate cancer associations: Biochemical and pathophysiological evidences [Oncotarget, 8, (2017) (30606-30616)] doi 10.18632/oncotarget.16725
- Author
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Quagliariello, V., Rossetti, S., Cavaliere, C., Di Palo, R., Lamantia, E., Castaldo, L., Nocerino, F., Ametrano, G., Cappuccio, F., Malzone, G., Montanari, M., Vanacore, D., Romano, F. J., Piscitelli, R., Iovane, G., Pepe, M. F., Berretta, M., D'Aniello, C., Perdona, S., Muto, P., Botti, G., Ciliberto, G., Veneziani, B. M., De Falco, F., Maiolino, P., Caraglia, M., Montella, M., Iaffaioli, R. V., and Facchini, G.
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endocrine disruptors ,nutrition ,prostate ,cancer ,metabolic syndrome - Published
- 2017
13. mCT analysis of mandibular molars before and after instrumentation by Reciproc files
- Author
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Ametrano G, Riccitiello F, Amato M, Formisano A, Muto M, Grassi R, Simeone M., VALLETTA, ALESSANDRA, Ametrano, G, Riccitiello, F, Amato, M, Formisano, A, Muto, M, Grassi, R, Valletta, Alessandra, and Simeone, M.
- Abstract
Cleaning and shaping are important section for the root canal treatment. A number of different methodologies have been developed to overcome these problems, including the introduction of rotary instruments nickel-titanium (NiTi). In endodontics NiTi have been shown to significantly reduce procedural errors compared to manual techniques of instrumentation. The efficiency of files is related to many factor. Although previous investigations that have used µCT analysis were hampered by insufficient resolution or projection incorrect. The new generation of μCT performance best offer, as micron resolution and accurate measurement software for evaluating the accurate anatomy of the root canal. The aim the paper was to evaluate the efficiency of Reciproc files in root canal treatment, evaluated before and after instrumentation by using μ-CT analysis.
- Published
- 2013
14. [µCT analysis of mandibular molars before and after instrumentation by Reciproc files
- Author
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Ametrano G, Riccitiello F, Amato M, Formisano A, Muto M, Valletta A, Simeone M., GRASSI, Roberto, Ametrano, G, Riccitiello, F, Amato, M, Formisano, A, Muto, M, Grassi, Roberto, Valletta, A, and Simeone, M.
- Subjects
Adult ,Male ,Titanium ,Tooth, Nonvital ,Dental Instruments ,Mandible ,Organ Size ,X-Ray Microtomography ,Reciproc ,Middle Aged ,µCT ,Molar ,Imaging, Three-Dimensional ,Nickel ,Dentin ,Humans ,Odontometry ,Female ,Dental Pulp Cavity ,Root Canal Preparation - Abstract
Cleaning and shaping are important section for the root canal treatment. A number of different methodologies have been developed to overcome these problems, including the introduction of rotary instruments nickel-titanium (NiTi). In endodontics NiTi have been shown to significantly reduce procedural errors compared to manual techniques of instrumentation. The efficiency of files is related to many factor. Although previous investigations that have used µCT analysis were hampered by insufficient resolution or projection incorrect. The new generation of μCT performance best offer, as micron resolution and accurate measurement software for evaluating the accurate anatomy of the root canal. The aim the paper was to evaluate the efficiency of Reciproc files in root canal treatment, evaluated before and after instrumentation by using μ-CT analysis.
- Published
- 2013
15. Ni-Ti rotary instruments surface alterations caused by heat sterilization: using AFM and SEM whit EDS
- Author
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Ametrano G., Formisano A., Ricittiello F., Amato M., SPAGNUOLO, GIANRICO, RENGO, SANDRO, Ametrano, G., Spagnuolo, Gianrico, Formisano, A., Ricittiello, F., Amato, M., and Rengo, Sandro
- Published
- 2011
16. Resistenza meccanica di ponti protesici in materiale polimerico rinforzato con fibre continue di vetro
- Author
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Ametrano G, Gloria A, Pagliari P, De Santis R, Ambrosio L, SIMEONE, MICHELE, RENGO, SANDRO, Ametrano, G, Gloria, A, Pagliari, P, De Santis, R, Simeone, Michele, Ambrosio, L, and Rengo, Sandro
- Published
- 2011
17. Effect of mineral trioxide aggregate on mesenchymal stem cells
- Author
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D'Antò V, Di Caprio MP, Ametrano G, SIMEONE, MICHELE, RENGO, SANDRO, SPAGNUOLO, GIANRICO, D'Antò, V, Di Caprio, Mp, Ametrano, G, Simeone, Michele, Rengo, Sandro, and Spagnuolo, Gianrico
- Abstract
INTRODUCTION: Mineral trioxide aggregate (MTA) is known to stimulate the hard tissue repair process. The purpose of this study was to evaluate the ability of MTA to support the adhesion, proliferation, and migration of human bone marrow-derived mesenchymal stem cells (hMSCs). METHODS: White ProRoot MTA and white Portland cement were mixed and left to set 24 hours. MSCs were cultured on the samples and observed after 24 hours by confocal laser scanning microscopy (CLSM) by using the cytoskeleton marker CellTracker. Cell proliferation was evaluated by means of alamar blue assay in the presence and absence of differentiation medium during a period of 28 days, and cells seeded on polystyrene culture wells were the control. To assess the effect on migratory ability of hMSCs, a transwell migration assay was performed for 18 hours, positioning MTA and Portland cement in 6-well plates and the cells in 8-μm pore inserts. RESULTS: hMSCs observed under CLSM showed attachment and spread activity on the upper surface of the MTA. Cell proliferation was significantly higher on MTA than on Portland cement. A rate proliferation increase of the MTA group compared with the control was observed after 14 days in presence of basic medium, whereas the same effect was reached after 21 days in presence of differentiation medium. Moreover, MTA was able to enhance cell migration significantly more than Portland cement. CONCLUSIONS: Our findings suggest that MTA was able to assist hMSC adhesion, growth, and migration.
- Published
- 2010
18. Mechanical Strength of Prosthetics Resin Bridges Reinforced with Continuous Fibers
- Author
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Ametrano G., Rengo C., Gloria A., Pagliari P., De Santis R., SPAGNUOLO, GIANRICO, RENGO, SANDRO, Ametrano, G., Rengo, C., Gloria, A., Pagliari, P., Spagnuolo, Gianrico, De Santis, R., and Rengo, Sandro
- Published
- 2010
19. Introduzione alle cellule staminali e all’endodonzia rigenerativa
- Author
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Di Caprio M.P., Ametrano G., SPAGNUOLO, GIANRICO, VALLETTA, ALESSANDRA, SIMEONE, MICHELE, RENGO, SANDRO, Di Caprio, M. P., Spagnuolo, Gianrico, Ametrano, G., Valletta, Alessandra, Simeone, Michele, and Rengo, Sandro
- Abstract
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors, scaffold materials and stem cells. This approach, called regenerative dentistry, is becoming a promising field of research which aims to replace, repair and regenerate dental tissues in order to restore the biological function that has been halted or compromised by injury or disease. The present review represent an introduction to stem cell biology, with special emphasis on stem cells derived from dental tissues. Since there has been a burst of interest, even at mass media level, for the potential possibility to built teeth in the laboratories, more patients are becoming aware of new treatment options, like those based on stem cells, as potentially available in the clinical practice. Therefore, the main objective of this article is to make the endodontic practitioners able to answer patient’s questions on stem cells, their properties and their potential use in regenerative endodontics and medicine.
- Published
- 2010
20. Analisi attraverso scansione AFM degli effetti superficiali degli irriganti canalari (NaOCl e EDTA) sui Protaper
- Author
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D’Apolito F., Ametrano G., SPAGNUOLO, GIANRICO, VALLETTA, ALESSANDRA, RENGO, SANDRO, D’Apolito, F., Ametrano, G., Spagnuolo, Gianrico, Valletta, Alessandra, and Rengo, Sandro
- Abstract
Objective. The aim of this study was to investigate, by using atomic force microscopy (AFM), the effects of sodium hypochlorite (NaOCl) and EDTA on the surface characteristics of ProTaper rotary nickel titanium instruments. Study design. A total of 20 ProTaper nickel-titanium files ( S1,S2,F1,F2), were divided in 5 groups: no immersion, immersion in 5.25% NaOCl for 5 or 10 minutes and immersion in 17% EDTA gel for 5 or 10 minutes. Twenty areas of the surface along 3-mm section at the tip of the files (perfect squares of 1μm x 1 μm) were analyzed by atomic force microscopy (AFM Perception, Assing, Italy) operating in contact mode under ambient condition. Three-dimensional images (400 × 400 lines) were processed by Gwiddion software and the roughness average (Ra) and the rootmean- square value (Rms) of the scanned surface profiles were recorded. The data were analyzed using Student t Test. Results. Mean Rms and Ra values for NaClO and EDTA measurements were statistically higher than the measurements on new files ( P < 0.05). Conclusion. The AFM results show that NaOCl and EDTA significantly increased the surface roughness of Protaper files.
- Published
- 2009
21. NaOCl and EDTA effects on the surface of nickel-titanium files
- Author
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Ametrano G., D’Antò V., D’apolito F., SPAGNUOLO, GIANRICO, SIMEONE, MICHELE, RENGO, SANDRO, Ametrano, G., D’Antò, V., D’Apolito, F., Spagnuolo, Gianrico, Simeone, Michele, and Rengo, Sandro
- Published
- 2009
22. Effect of mineral trioxide aggregate on mensenchymal stemm cells
- Author
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Di Caprio M.P., D’Antò V., Ametrano G., Ambrosio L., SPAGNUOLO, GIANRICO, SIMEONE, MICHELE, RENGO, SANDRO, Di Caprio, M. P., D’Antò, V., Spagnuolo, Gianrico, Ametrano, G., Simeone, Michele, Ambrosio, L., and Rengo, Sandro
- Published
- 2009
23. Influenza di un nuovo desensibilizzante sulla forza di adesione al substrato dentinale
- Author
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Ametrano G., Rengo C., D’Antò V., De Santis R.*., SPAGNUOLO, GIANRICO, RENGO, SANDRO, Ametrano, G., Rengo, C., D’Antò, V., Spagnuolo, Gianrico, De Santis, R. *., and Rengo, Sandro
- Published
- 2009
24. Analisys of surface alterations of Ni-Ti rotary instruments caused by autoclave sterilization
- Author
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Amato, Massimo, di Caprio, M. P., Ametrano, G., Spagnuolo, G., Formisano, A., and Rengo, S.
- Published
- 2012
25. Ni-Ti rotary instruments surface alterations caused by heat sterilization
- Author
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Ametrano, G., Spagnuolo, G., Formisano, A., Riccitiello, F., Amato, Massimo, and Rengo, S.
- Published
- 2011
26. Marginal Leakage of Class V Composite Restorations Assessed Using Microcomputed Tomography and Scanning Electron Microscope
- Author
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Rengo, C, primary, Goracci, C, primary, Ametrano, G, primary, Chieffi, N, primary, Spagnuolo, G, primary, Rengo, S, primary, and Ferrari, M, primary
- Published
- 2015
- Full Text
- View/download PDF
27. Micro-Computed Tomographic Analysis of Mandibular First Molars Anatomy
- Author
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Spagnuolo G, Ametrano G, DAntò V, DApolito F, Schweikl H, and Simeone M
- Published
- 2010
28. Rectal/urinary toxicity after hypofractionated vs. conventional radiotherapy in high risk prostate cancer: systematic review and meta analysis.
- Author
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DI FRANCO, R., BORZILLO, V., RAVO, V., AMETRANO, G., CAMMAROTA, F., ROSSETTI, S., ROMANO, F. J., D'ANIELLO, C., CAVALIERE, C., IOVANE, G., PORRICELLI, M. A., MUTO, M., BERRETTA, M., FACCHINI, G., and MUTO, P.
- Abstract
OBJECTIVE: The aim of our report was to review the literature concerning the toxicity of radiation therapy in patients treated for high-risk prostate cancer, and to evaluate the differences in toxicity between conventional fractionation and hypofractionated treatments, in view of different techniques used in high-risk prostate cancer patients. MATERIALS AND METHODS: PubMed database has been explored for studies concerning acute and late urinary/gastrointestinal toxicity in high-risk prostate cancer patients treated with radiotherapy. Prospective studies, concerning potential relationship between acute/late genitourinary (GU)/ gastrointestinal (GI) toxicity and prostate radiotherapy in patients with high-risk prostate cancer, were included in the final analysis. Data collected from single arm, phase II non-randomized and randomized studies have been evaluated to perform odds ratio for toxicity risk. Furthermore, meta-analysis randomized prospective trials were considered suitable because they had recruited high-risk prostate cancer patients who didn't undergo surgery, with available data on ≥ G2 toxicity frequency. RESULTS: The initial search provided 606 results, but only 35 manuscripts met all eligibility requirements and were included in this report. In order to perform odds ratio we observed a decrease in late gastrointestinal toxicity for patients treated with hypofractionated schemes compared to CV treated ones. Among patients who underwent conventional treatment, SIB seemed to decrease acute genitourinary side effects; SIB-Hypo treated patients suffered less toxicity than patients treated with hypofractionated-sequential boost schemes. Hypo-SIB1 schemes would seem less toxic in terms of acute gastrointestinal and late genitourinary side effects than CV-SIB. Therefore, our focus shifted to 6 clinical trials evaluating genitourinary and gastrointestinal toxicity in patients who had been randomized to receive conventional fractionation or hypofractionated treatment, in both cases with IMRT technology. Our meta-analysis of these randomized trials involving patients with high-risk prostate cancer showed a statistically significant increase in late genitourinary toxicity for hypo-treated patients; no difference was observed in acute genitourinary/gastrointestinal toxicity, and in late gastrointestinal toxicity. CONCLUSIONS: Our analysis doesn't want to establish a definitive truth; very few trials assessed only high risk-class patients. Our purpose is to stimulate further randomized prospective trials focusing both on the effectiveness and toxicity profile (toxicity/effectiveness ratio), taking into account the use of different technologies and doses. [ABSTRACT FROM AUTHOR]
- Published
- 2017
29. Effects of sodium hypochlorite and ethylenediaminetetraacetic acid on rotary nickel-titanium instruments evaluated using atomic force microscopy
- Author
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Ametrano, G., primary, D’Antò, V., additional, Di Caprio, M. P., additional, Simeone, M., additional, Rengo, S., additional, and Spagnuolo, G., additional
- Published
- 2010
- Full Text
- View/download PDF
30. μcT analysis of mandibular molars before and after instrumentation by Reciproc files,Analisi anatomiche di molari mandibolari pre- e post-strumentazione con Reciproc mediante μtC
- Author
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Ametrano, G., Riccitiello, F., Amato, M., Formisano, A., Massimo Muto, Grassi, R., Valletta, A., and Simeone, M.
31. Rectal/urinary toxicity after hypofractionated vs. conventional radiotherapy in high risk prostate cancer: systematic review and meta analysis
- Author
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Di Franco R, Borzillo V, Ravo V, Ametrano G, Cammarota F, Rossetti S, Fj, Romano, D'Aniello C, Cavaliere C, GELSOMINA IOVANE, Ma, Porricelli, Muto M, and Muto P
32. Growth, Anatomical, and Biochemical Responses of the Space Farming Candidate Brassica rapa L. Microgreens to Low-LET Ionizing Radiation
- Author
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Sara De Francesco, Chiara Amitrano, Ermenegilda Vitale, Giulia Costanzo, Mariagabriella Pugliese, Cecilia Arrichiello, Gianluca Ametrano, Paolo Muto, Carmen Arena, Veronica De Micco, De Francesco, S., Amitrano, C., Vitale, E., Costanzo, G., Pugliese, M., Arrichiello, C., Ametrano, G., Muto, P., Arena, C., and De Micco, V.
- Subjects
exo-agriculture ,food countermeasure ,ionizing radiation ,low-LET radiation ,microgreens ,morpho-anatomical traits ,phytochemicals ,space exploration ,Plant Science ,Horticulture - Abstract
An adequate and balanced diet is fundamental in preserving the health of astronauts from several space-induced diseases. Therefore, the integration of a diet with fresh food, rich in bioactive compounds such as microgreens produced directly onboard, may be useful in space for human nutrition. However, ionizing radiation (IR) in space represents a significant hindrance for organisms, with potential critical outcomes on plant morpho-anatomical, eco-physiological, and biochemical aspects, depending on the plant and IR features (e.g., species, developmental stage, IR dose, and type). In this study, we analyzed the effect of different doses of X-rays (0-control, 0.3, 1, 10, 20, and 30 Gy) on the morpho-anatomical and nutritional traits of microgreens of Brassica rapa L., irradiated at the stage of germinated seeds. After the irradiation, microgreens were cultivated in controlled conditions. At harvest, the morpho-biometric traits were analyzed, along with the leaf functional anatomical traits and the phytochemical content of the aboveground biomass. The results showed that X-ray exposure does not induce detrimental effects on growth, while it stimulates the production of antioxidants, improving plant defense and nutritional value. The overall results support the idea of using this species in space as a supplemental functional food.
- Published
- 2023
- Full Text
- View/download PDF
33. Evaluation of surface roughness of orthodontic wires by means of atomic force microscopy
- Author
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Roberto Rongo, Roberto Martina, Gianrico Spagnuolo, Gianluca Ametrano, Rosa Valletta, Vincenzo D'Antò, Paolo Manzo, Sergio Paduano, D'Antò, V, Rongo, R, Ametrano, G, Spagnuolo, G, Manzo, P, Martina, Roberto, Paduano, S, Valletta, R., D'Antò, V., Rongo, R., Ametrano, G., Spagnuolo, Gianrico, Manzo, P., Paduano, S., and Valletta, Rosa
- Subjects
Analysis of Variance ,Materials science ,Friction ,Orthodontic wire ,Surface Properties ,Atomic force microscopy ,Analytical chemistry ,chemistry.chemical_element ,Orthodontics ,Nanotechnology ,Original Articles ,Surface finish ,Microscopy, Atomic Force ,Standard deviation ,Root mean square ,chemistry ,Materials Testing ,Microscopy ,Orthodontic Wires ,Surface roughness ,Dental Alloys ,Titanium - Abstract
Objective: To compare the surface roughness of different orthodontic archwires. Materials and Methods: Four nickel-titanium wires (Sentalloy®, Sentalloy® High Aesthetic, Titanium Memory ThermaTi Lite®, and Titanium Memory Esthetic®), three β-titanium wires (TMA®, Colored TMA®, and Beta Titanium®), and one stainless-steel wire (Stainless Steel®) were considered for this study. Three samples for each wire were analyzed by atomic force microscopy (AFM). Three-dimensional images were processed using Gwiddion software, and the roughness average (Ra), the root mean square (Rms), and the maximum height (Mh) values of the scanned surface profile were recorded. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by Tukey's post hoc test (P < .05). Results: The Ra, Rms, and Mh values were expressed as the mean ± standard deviation. Among as-received archwires, the Stainless Steel (Ra = 36.6 ± 5.8; Rms = 48 ± 7.7; Mh = 328.1 ± 64) archwire was less rough than the others (ANOVA, P < .05). The Sentalloy High Aesthetic was the roughest (Ra = 133.5 ± 10.8; Rms = 165.8 ± 9.8; Mh = 949.6 ± 192.1) of the archwires. Conclusions: The surface quality of the wires investigated differed significantly. Ion implantation effectively reduced the roughness of TMA. Moreover, Teflon®-coated Titanium Memory Esthetic was less rough than was ion-implanted Sentalloy High Aesthetic.
- Published
- 2012
34. Dosimetric comparison among cyberknife, helical tomotherapy and VMAT for hypofractionated treatment in localized prostate cancer
- Author
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Maria Quarto, Savino Federica, Filomena Loffredo, V. Borzillo, Rossella Di Franco, Matteo Muto, Muto Paolo, Marcello Serra, Gianluca Ametrano, Serra, M., Ametrano, G., Borzillo, V., Quarto, M., Muto, M., Di Franco, R., Federica, S., Loffredo, F., and Paolo, M.
- Subjects
Male ,medicine.medical_treatment ,Rectum ,VMAT ,Radiosurgery ,Tomotherapy ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Cyberknife ,Quality Improvement Study ,cyberknife ,medicine ,Humans ,030212 general & internal medicine ,helical tomotherpy ,Radiometry ,radiotherapy ,Image-guided radiation therapy ,medicine.diagnostic_test ,business.industry ,Prostate ,Prostatic Neoplasms ,Magnetic resonance imaging ,General Medicine ,prostate cancer ,medicine.disease ,Magnetic Resonance Imaging ,Conformity index ,Radiation therapy ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Prostatic Neoplasm ,stereotactic radiotherapy ,Radiation Dose Hypofractionation ,Radiotherapy, Intensity-Modulated ,business ,Nuclear medicine ,Research Article ,Human - Abstract
Hypofractionation for localized prostate cancer treatment is rapidly spreading in the medical community and it is supported by radiobiological evidences (lower α/β ratio compared with surrounding tissues). Stereotactic body radiation therapy (SBRT) is a technique to administer high doses with great precision, which is commonly performed with CyberKnife (CK) in prostate cancer treatment. Since the CyberKnife (CK) is not available at all radiotherapy center, alternative SBRT techniques are available such as Volumetric Modulated Arc Therapy (VMAT) and Helical Tomotherapy (HT). The aim of the present study was to compare the dosimetric differences between the CK, VMAT, and HT plans for localized prostate cancer treatment. Seventeenpatients have been recruited and replanned using VMAT and HT to this purpose: they received the treatment using the CK with a prescription of 36.25 Gy in 5 fractions; bladder, rectum and penis bulb were considered as organs at risk (OAR). In order to compare the techniques, we considered DVHs, PTV coverage, Conformity Index and new Conformity Index, Homogeneity Index, beam-on time and OARs received dose. The 3 treatments methods showed a comparable coverage of the lesion (PTV 95%: 99.8 ± 0.4% CK; 98.5 ± 0.8% VMAT; 99.4 ± 0.5% HT. P
- Published
- 2020
35. Lithium chloride increases sensitivity to photon irradiation treatment in primary mesenchymal colon cancer cells
- Author
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Antonietta Aversano, Gianluca Ametrano, Valeria Valente, Paolo Muto, Paolo Delrio, Mimmo Turano, Giovanna Maria Pierantoni, Marina De Rosa, Paola Izzo, Andrea Conte, Patrizia Riccio, Francesca Cammarota, Cammarota, F., Conte, A., Aversano, A., Muto, P., Ametrano, G., Riccio, P., Turano, M., Valente, V., Delrio, P., Izzo, P., Pierantoni, G. M., and De Rosa, M.
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0301 basic medicine ,Radiation-Sensitizing Agents ,Cancer Research ,Cell Survival ,Colorectal cancer ,medicine.medical_treatment ,Mesenchymal colon cancer cells ,Radiation Tolerance ,Biochemistry ,Radiotherapy, High-Energy ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Survivin ,Genetics ,medicine ,Humans ,Molecular Biology ,Cell Proliferation ,Photons ,Oncogene ,Chemistry ,apoptosis ,Cancer ,Apoptosi ,Articles ,Cell cycle ,Cancer drug resistance ,medicine.disease ,High energy photon irradiation ,Molecular medicine ,Radiation therapy ,030104 developmental biology ,Lithium chloride ,Oncology ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Cancer cell ,Cancer research ,Molecular Medicine ,Signal Transduction - Abstract
Colorectal cancer (CRC) is the third most prevalent type of cancer worldwide. It is also the second most common cause of cancer‑associated mortality; it accounted for about 9.2% of all cancer deaths in 2018, most of which were due to resistance to therapy. The main treatment for CRC is surgery, generally associated with chemotherapy, radiation therapy and combination therapy. However, while chemo‑radiotherapy kills differentiated cancer cells, mesenchymal stem‑like cells are resistant to this treatment, and this can give rise to therapy‑resistant tumors. Our previous study isolated T88 primary colon cancer cells from a patient with sporadic colon cancer. These cells exhibited mesenchymal and epithelial features, high levels of epithelial‑to‑mesenchymal transition transcription factors, and stemness markers. In addition, it was revealed that lithium chloride (LiCl), a specific glycogen synthase kinase (GSK)‑3β inhibitor, induced both the mesenchymal‑to‑epithelial transition and differentiation, and also reduced cell migration, stemness features and cell plasticity in these primary colon cancer cells. The aim of the present study was to investigate the effect of LiCl treatment on the viability of primary colon cancer cells exposed to 7 Gy delivered by high‑energy photon beams, which corresponds to 6 megavolts of energy. To achieve this aim, the viability of irradiated T88 cells was compared with that of irradiated T88 cells pre‑treated with LiCl. As expected, it was observed that LiCl sensitized primary colon cancer cells to high‑energy photon irradiation treatment. Notably, the decrease in cell viability was greater with combined therapy than with irradiation alone. To explore the molecular basis of this response, the effect of LiCl on the expression of Bax, p53 and Survivin, which are proteins involved in the apoptotic mechanism and in death escape, was analyzed. The present study revealed that LiCl upregulated the expression of pro‑apoptotic proteins and downregulated the expression of proteins involved in survival. These effects were enhanced by high‑energy photon irradiation, suggesting that LiCl could be used to sensitize colon cancer cells to radiation therapy.
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- 2020
36. Microcomputed Tomography Analysis of Mesiobuccal Orifices and Major Apical Foramen in First Maxillary Molars
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Sandro Rengo, Francesco Riccitiello, Gianrico Spagnuolo, Anna Formisano, Gianluca Ametrano, Vincenzo D'Antò, Massimo Amato, Michele Simeone, Spagnuolo, G, Ametrano, G, D'Antò, V, Formisano, A, Simeone, Michele, Riccitiello, Francesco, Amato, M, and Rengo, Sandro
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Molar ,anatomy ,business.industry ,Root canal ,First maxillary molar ,Dentistry ,Anatomy ,Microcomputed tomography ,Canal system ,Article ,Apex (geometry) ,MB2 ,medicine.anatomical_structure ,µCT ,μCT ,μCT, Anatomy, First maxillary molar, MB2 ,medicine ,Foramen ,Maxillary first molar ,Apical foramen ,business ,General Dentistry - Abstract
Objective: Aim of the study was to determined by microcomputed tomography (µCT) the horizontal distance between the main (MB1) and the second mesiobuccal canal (MB2) orifices, the vertical distance between the MB1 and MB2 orifices planes, and the distance between the anatomic apex and major apical foramen (AF). Furthermore, we characterized the entire internal and external anatomy of the MB, distalbuccal (DB) and palatal (P) maxillary first molars roots. Materials and Methods: Twenty-two intact extracted first maxillary molars were scanned by X-ray computed transaxial µCT and then 2D and 3D images were processed and analyzed. Results: The results showed that 77.27% of the mesiobuccal (MB) roots presented a second MB canal, and 29.41% of the MB2 were independent from the MB1 canals. In 15 teeth, there were three root canal orifices on the chamber floor, and 10 of these teeth presented MB2 canals. The mean vertical distance between the MB1 and MB2 planes was 1.68 ± 0.83 mm. Seven teeth had four orifices. The mean horizontal interorificial distance between the MB1 and MB2 orifices was 1.21 ± 0.5 mm. Accessory canals were observed in 33.33% of the roots, loops in 6.06%, while isthmuses were found in 15 of the 22 MB roots. Of the total roots, 74.24% presented one foramen, while all of the roots showed a major apical foramen that was not coincident with the anatomic apex. Conclusions: Our µCT analysis provided interesting features on the horizontal and vertical distance between the MB1 and MB2 orifices and on the distance of AF and anatomic apex. Clinical Relevance: These results have an important clinical value because might support the endodontist in the recruitment, negotiation and obturation of maxillary first molar canal system.
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- 2012
37. Marginal Leakage of Class V Composite Restorations Assessed Using Microcomputed Tomography and Scanning Electron Microscope
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Gianrico Spagnuolo, Gianluca Ametrano, Marco Ferrari, Nicoletta Chieffi, Cecilia Goracci, S Rengo, Carlo Rengo, Rengo, C, Goracci, C, Ametrano, G, Chieffi, N, Spagnuolo, Gianrico, Rengo, Sandro, and Ferrari, M.
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Materials science ,Scanning electron microscope ,Composite number ,Dentistry ,Dental Caries ,Composite Resins ,Humans ,Dental Enamel ,Dental Restoration, Permanent ,General Dentistry ,Leakage (electronics) ,Dental Leakage ,business.industry ,Medicine (all) ,Marginal leakage ,X-Ray Microtomography ,Microcomputed tomography ,Dental Marginal Adaptation ,Molar ,Dentistry (all) ,Resin Cements ,Dentin ,Microscopy, Electron, Scanning ,business ,Dental Cavity Preparation ,Biomedical engineering - Abstract
SUMMARY Objective The aim of the study was to compare in Class V composite restorations marginal leakage measurements obtained with microcomputed tomography (micro-CT) and scanning electron microscopy (SEM) observations. Methods Class V cavities were prepared on 10 human molars and restored using Optibond FL (Kerr, Orange, CA, USA) and Premise Flowable (Kerr). Sealing ability was evaluated by assessing silver-nitrate penetration depth along enamel and dentin margins. Leakage was quantified using a scoring system. Micro-CT analysis provided 502 cross-sectional images for each tooth. Microleakage evaluation was performed first on three cross-sections corresponding to the sections examined by SEM, then on all 502 of the obtained micro-CT images. SEM observations were performed first at 20× magnification, then, if showing a zero score, at 80× magnification. Enamel and dentin microleakage scores assigned to corresponding sections through micro-CT and SEM (20×) were compared (Wilcoxon signed-rank test, α=0.05). Results No statistically significant difference in leakage scores emerged between micro-CT and 20×-magnification SEM. Eight tooth sections that were given a zero score under SEM at 20× magnification showed to be infiltrated at the higher magnification (80×). For five teeth a higher score was assigned following scanning of 502 cross-sections than based on the observation of three sections. Conclusions Micro-CT presents as a valid, nondestructive in vitro method to quantitatively evaluate marginal leakage of adhesive restorations.
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- 2015
38. Effects of intraoral aging on surface properties of coated nickel-titanium archwires
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Roberto Rongo, Vincenzo D'Antò, Gianluca Ametrano, Antonio Gloria, Gianrico Spagnuolo, Rosa Valletta, Sergio Paduano, Angela Galeotti, Rongo, R, Ametrano, G, Gloria, A, Spagnuolo, G, Galeotti, A, Paduano, S, Valletta, R, and D'Antò, Vincenzo.
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Dental Stress Analysis ,Ceramics ,Materials science ,Time Factors ,Friction ,Orthodontic Brackets ,Polymers ,Surface Properties ,Surface treatment ,Retrieved analysis ,chemistry.chemical_element ,Orthodontics ,Microscopy, Atomic Force ,Orthodontic archwire ,law.invention ,Monocrystalline silicon ,Atomic force microscopy ,Surface roughness ,Coated Materials, Biocompatible ,law ,Nickel ,Microscopy ,Materials Testing ,Orthodontic Wires ,Humans ,Orthodontic Appliance Design ,Composite material ,Retrieved analysi ,Platinum ,Titanium ,Mouth ,Surface roughne ,Original Articles ,Stainless Steel ,chemistry ,Nickel titanium ,Microscopy, Electron, Scanning ,Steel plates ,Gold Alloys ,Electron microscope ,Dental Alloys - Abstract
Objective: To evaluate the effects of intraoral aging on surface properties of esthetic and conventional nickel-titanium (NiTi) archwires. Materials and Methods: Five NiTi wires were considered for this study (Sentalloy, Sentalloy High Aesthetic, Superelastic Titanium Memory Wire, Esthetic Superelastic Titanium Memory Wire, and EverWhite). For each type of wire, four samples were analyzed as received and after 1 month of clinical use by an atomic force microscope (AFM) and a scanning electronic microscope (SEM). To evaluate sliding resistance, two stainless steel plates with three metallic or three monocrystalline brackets, bonded in passive configuration, were manufactured; four as-received and retrieved samples for every wire were pulled five times at 5 mm/min for 1 minute by means of an Instron 5566, recording the greatest friction value (N). Data were analyzed by one-way analysis of variance and by Student's t-test. Results: After clinical use, surface roughness increased considerably. The SEM images showed homogeneity for the as-received control wires; however, after clinical use esthetic wires exhibited a heterogeneous surface with craters and bumps. The lowest levels of friction were observed with the as-received Superelastic Titanium Memory Wire on metallic brackets. When tested on ceramic brackets, all the wires exhibited an increase in friction (t-test; P < .05). Furthermore, all the wires, except Sentalloy, showed a statistically significant increase in friction between the as-received and retrieved groups (t-test; P < .05). Conclusion: Clinical use of the orthodontic wires increases their surface roughness and the level of friction.
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- 2013
39. Effect of autoclaving on the surfaces of TiN -coated and conventional nickel-titanium rotary instruments
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Carlo Rengo, Gianrico Spagnuolo, Gianluca Ametrano, Francesco Riccitiello, Massimo Amato, Vincenzo D'Antò, Michele Simeone, Spagnuolo, G., Ametrano, G., D'Antò, V., Rengo, C., Simeone, Michele, Riccitiello, F., and Amato, M.
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Dental Stress Analysis ,Materials science ,Scanning electron microscope ,Surface Properties ,chemistry.chemical_element ,Surface finish ,Nitride ,Microscopy, Atomic Force ,NiTi ,Autoclave ,chemistry.chemical_compound ,Coated Materials, Biocompatible ,Nickel ,TiN ,Materials Testing ,General Dentistry ,Titanium ,autoclave ,Metallurgy ,Spectrometry, X-Ray Emission ,Autoclave, NiTi, Sterilization, TiN ,sterilization ,Titanium nitride ,Steam ,chemistry ,Nickel titanium ,Microscopy, Electron, Scanning ,Tin ,Root Canal Preparation ,Dental Alloys - Abstract
Aim To evaluate the effects of repeated autoclave sterilization cycles on surface topography of conventional nickel–titanium ( NiTi ) and titanium nitride ( TiN )-coated rotary instruments. Methodology A total of 60 NiTi rotary instruments, 30 ProTaper (Dentsply Maillefer) and 30 TiN -coated AlphaKite (Komet/Gebr. Brasseler), were analysed. Instruments were evaluated in the as-received condition and after 1, 5 and 10 sterilization cycles. After sterilization, the samples were observed using scanning electron microscope (SEM), and surface chemical analysis was performed on each instrument with energy dispersive X-ray spectroscopy (EDS). Moreover, the samples were analysed by atomic force microscopy (AFM), and roughness average (Ra) and the root mean square value (RMS) of the scanned surface profiles were recorded. Data were analysed by means of anova followed by Tukey's test. Result Scanning electron microscope observations revealed the presence of pitting and deep milling marks in all instruments. EDS analysis confirmed that both types of instruments were composed mainly of nickel and titanium, whilst AlphaKite had additional nitride. After multiple autoclave sterilization cycles, SEM examinations revealed an increase in surface alterations, and EDS values indicated changes in chemical surface composition in all instruments. Ra and RMS values of ProTaper significantly increased after 5 (P = 0.006) and 10 cycles (P = 0.002) with respect to the as-received instruments, whilst AlphaKite showed significant differences compared with the controls after 10 cycles (P = 0.03). Conclusions Multiple autoclave sterilization cycles modified the surface topography and chemical composition of conventional and TiN -coated NiTi rotary instruments.
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- 2012
40. Advanced composite substrates for hard tissue regeneration
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U. D'Amora, T. Russo, A. Gloria, V. D'Antò, G. Ametrano, F. Bollino, R. De Santis, G. Ausanio, M. Catauro, S. Rengo, L. Ambrosio., D’Amora, U, Russo, T, Gloria, A, D’Antò, V, Ametrano, G, Bollino, F, De Santis, R, Ausanio, G, Catauro, Michelina, Rengo, S, and Ambrosio, L.
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- 2011
41. Poly(e-caprolactone) reinforced with sol-gel synthesized organic-inorganic hybrid fillers as composite substrates for tissue engineering
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Teresa Russo, Sandro Rengo, Antonio Gloria, Luigi Ambrosio, Roberto De Santis, Vincenzo D'Antò, Ugo D'Amora, Giovanni Ausanio, Flavia Bollino, Gianluca Ametrano, Michelina Catauro, Russo, Teresa, Gloria, Antonio, D’Antò, V., D’Amora, U., Ametrano, Gianluca, Bollino, F., DE SANTIS, Roberto, Ausanio, Giovanni, Catauro, M., Rengo, Sandro, Ambrosio, Luigi, Russo, T, Gloria, A, Dantò, V, Damora, U, Ametrano, G, Bollino, Flavia, DE SANTIS, R, Ausanio, G, Catauro, Michelina, Rengo, S, and Ambrosio, L.
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Inorganic Chemical ,chemistry.chemical_classification ,Titanium ,Materials science ,Tissue Engineering ,Polyesters ,Composite number ,chemistry.chemical_element ,Polymer ,Microscopy, Atomic Force ,Polyester ,chemistry.chemical_compound ,chemistry ,Tissue engineering ,Inorganic Chemicals ,Polymethyl Methacrylate ,Zirconium ,Composite material ,Organic Chemicals ,Caprolactone ,Gels ,Sol-gel - Abstract
Purpose: The importance of polymer-based composite materials to make multifunctional substrates for tissue engineering and the strategies to improve their performances have been stressed in the literature. Bioactive features of sol-gel synthesized poly(epsilon-caprolactone)/TiO(2) or poly(epsilon-caprolactone)/ZrO(2) organic-inorganic hybrid materials are widely documented. Accordingly, the aim of this preliminary research was to develop advanced composite substrates consisting of a poly(epsilon-caprolactone) matrix reinforced with sol-gel synthesized PCL/TiO(2) or PCL/ZrO(2) hybrid fillers. Methods: Micro-computed tomography and atomic force microscopy analyses allowed to study surface topography and roughness. On the other hand, mechanical and biological performances were evaluated by small punch tests and Alamar Blue (TM) assay, respectively. Results: Micro-computed tomography and atomic force microscopy analyses highlighted the effect of the preparation technique. Results from small punch tests and Alamar Blue (TM) assay evidenced that PCL reinforced with Ti2 (PCL=12, TiO(2) = 88 wt%) and Zr2 (PCL=12, ZrO(2) = 88 wt%) hybrid fillers provided better mechanical and biological performances. Conclusions: PCL reinforced with Ti2 (PCL=12, TiO(2) = 88 wt%) and Zr2 (PCL=12, ZrO(2) = 88 wt%) hybrid fillers could be considered as advanced composite substrates for hard tissue engineering.
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- 2010
42. Sintesi e caratterizzazione di materiali ibridi nanostrutturati per la realizzazione di scalffold per l’ingegneria per l’ingegneria dei tessuti craniofacciali
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V. D'Antò, R. De Santis, A. Gloria, F. Bollino, D. Verardi, G. Spagnuolo, G. Ametrano, R. Valletta, R. Martina, L. Ambrosio e. S. Rengo, CATAURO, Michelina, D'Antò, V., De Santis, R., Catauro, Michelina, Gloria, A., Bollino, F., Verardi, D., Spagnuolo, G., Ametrano, G., Valletta, R., Martina, R., and Rengo, L. Ambrosio e. S.
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- 2009
43. Stereotactic body radiotherapy with CyberKnife ® System for low- and intermediate-risk prostate cancer: clinical outcomes and toxicities of CyPro Trial.
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Borzillo V, Scipilliti E, Pezzulla D, Serra M, Ametrano G, Quarto G, Perdonà S, Rossetti S, Pignata S, Crispo A, Di Gennaro P, D'Alesio V, Arrichiello C, Buonanno F, Mercogliano S, Russo A, Tufano A, Di Franco R, and Muto P
- Abstract
Simple Summary: Stereotactic body radiotherapy (SBRT) of 35-36.25 Gy in five fractions with the CyberKnife System yields excellent control with low toxicity in low-intermediate-risk prostate cancer patients. We found no differences in biochemical control and overall survival in relation to dose. There were no significant differences in toxicity or quality of life between the two groups., Aims: Stereotactic body radiotherapy (SBRT) is an emerging therapeutic approach for low- and intermediate-risk prostate cancer. We present retrospective data on biochemical control, toxicity, and quality of life of CyPro Trial., Materials and Methods: A total of 122 patients with low- and intermediate-risk prostate cancer were treated with the CyberKnife System at a dose of 35 Gy or 36.25 Gy in five fractions. Biochemical failure (BF)/biochemical disease-free survival (bDFS) was defined using the Phoenix method (nadir + 2 ng/ml). Acute/late rectal and urinary toxicities were assessed by the Radiation Therapy Oncology Group (RTOG) toxicity scale. Quality of life (QoL) was assessed by the European Organisation for Research and Treatment of Cancer (EORTC) QLQ C30 and PR25. International Erectile Function Index-5 (IIEF5) and International Prostate Symptom Score (IPSS) questionnaires were administered at baseline, every 3 months after treatment during the first years, and then at 24 months and 36 months., Results: The 1-, 2-, and 5-year DFS rates were 92.9%, 92.9%, and 92.3%, respectively, while the 1-, 2-, and 5-year bDFS rates were 100%, 100%, and 95.7%, respectively. With regard to risk groups or doses, no statistically significant differences were found in terms of DFS or bDFS. Grade 2 urinary toxicity was acute in 10% and delayed in 2% of patients. No Grade 3 acute and late urinary toxicity was observed. Grade 2 rectal toxicity was acute in 8% and late in 1% of patients. No Grade 3-4 acute and late rectal toxicity was observed. Grade 2 acute toxicity appeared higher in the high-dose group (20% in the 36.25-Gy group versus 3% in the 35-Gy group) but was not statistically significant., Conclusion: Our study confirms that SBRT of 35-36.25 Gy in five fractions with the CyberKnife System produces excellent control with low toxicity in patients with low-intermediate-risk prostate cancer. We found no dose-related differences in biochemical control and overall survival. Further confirmation of these results is awaited through the prospective phase of this study, which is still ongoing., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Borzillo, Scipilliti, Pezzulla, Serra, Ametrano, Quarto, Perdonà, Rossetti, Pignata, Crispo, Di Gennaro, D’Alesio, Arrichiello, Buonanno, Mercogliano, Russo, Tufano, Di Franco and Muto.)
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- 2023
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44. Reirradiation of Locally Recurrent Prostate Cancer with Cyberknife ® System or Volumetric Modulated Arc Therapy (VMAT) and IGRT-Clarity ® : Outcomes, Toxicities and Dosimetric Evaluation.
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Di Franco R, Borzillo V, Scipilliti E, Ametrano G, Serra M, Arrichiello C, Savino F, De Martino F, D'Alesio V, Cammarota F, Crispo A, Pignata S, Rossetti S, Quarto G, and Muto P
- Abstract
The management of prostate cancer recurrence following external beam radiotherapy is not defined yet. Stereotaxic body reirradiation therapy showed encouraging results for local and biochemical control. From April 2017 to December 2020, 29 patients with prostate cancer recurrence were collected, joining the retrospective studies CyPro (prot. 46/19 OSS) and CLARO (Prot. 19/20 OSS) trials. Patients received Cyberknife
® treatment (17 pts) or alternatively VMAT (Volumetric Modulated Arc Technique) therapy by IGRT (Image-Guided Radiation Therapy)/Clarity® (12 pts). By comparing the reirradiation of two groups, urinary (GU), rectal (GI) toxicities, and biochemical control were investigated. Further, the two techniques were dosimetrically compared by rival plans. The VMAT-IGRT Clarity® treatments were replanned with an optimized template developed for prostate VMAT-SBRT in FFF mode keeping the same dose and fractionation scheduled for Cyberknife Group (30 Gy in 5 fx, at 80% isodose). In the CK group, 23% of patients experienced grade 2 acute GU, while 6% grade 2 acute GI. In the VMAT-Clarity® group, acute GU toxicity was recorded in 17%, while for 8% grade 2 late toxicity was recorded. The dosimetric analysis shows that the VMAT-FFF allows to deliver a biological equivalent dose to CK, with the advantage of reducing the likelihood of toxicities arising.- Published
- 2022
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45. SBRT for Localized Prostate Cancer: CyberKnife vs. VMAT-FFF, a Dosimetric Study.
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Serra M, De Martino F, Savino F, D'Alesio V, Arrichiello C, Quarto M, Loffredo F, Di Franco R, Borzillo V, Muto M, Ametrano G, and Muto P
- Abstract
In recent years, stereotactic body radiation therapy (SBRT) has gained popularity among clinical methods for the treatment of medium and low risk prostate cancer (PCa), mainly as an alternative to surgery. The hypo-fractionated regimen allows the administration of high doses of radiation in a small number of fractions; such a fractionation is possible by exploiting the different intrinsic prostate radiosensitivity compared with the surrounding healthy tissues. In addition, SBRT treatment guaranteed a better quality of life compared with surgery, avoiding risks, aftermaths, and possible complications. At present, most stereotactic prostate treatments are performed with the CyberKnife (CK) system, which is an accelerator exclusively dedicated for stereotaxis and it is not widely spread in every radiotherapy centre like a classic linear accelerator (LINAC). To be fair, a stereotactic treatment is achievable also by using a LINAC through Volumetric Modulated Arc Therapy (VMAT), but some precautions must be taken. The aim of this work is to carry out a dosimetric comparison between these two methodologies. In order to pursue such a goal, two groups of patients were selected at Instituto Nazionale Tumori-IRCCS Fondazione G. Pascale: the first group consisting of ten patients previously treated with a SBRT performed with CK; the second one was composed of ten patients who received a hypo-fractionated VMAT treatment and replanned in VMAT-SBRT flattening filter free mode (FFF). The two SBRT techniques were rescaled at the same target coverage and compared by normal tissue sparing, dose distribution parameters and delivery time. All organs at risk (OAR) constraints were achieved by both platforms. CK exhibits higher performances in terms of dose delivery; nevertheless, the general satisfying dosimetric results and the significantly shorter delivery time make VMAT-FFF an attractive and reasonable alternative SBRT technique for the treatment of localized prostate cancer.
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- 2022
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46. Prospective Evaluation of Radiotherapy-Induced Immunologic and Genetic Effects in Colorectal Cancer Oligo-Metastatic Patients with Lung-Limited Disease: The PRELUDE-1 Study.
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Ottaiano A, Petito A, Santorsola M, Gigantino V, Capuozzo M, Fontanella D, Di Franco R, Borzillo V, Buonopane S, Ravo V, Scipilliti E, Totaro G, Serra M, Ametrano G, Penta R, Tatangelo F, Scognamiglio G, Di Mauro A, Di Bonito M, Napolitano M, Scala S, Rea G, Santagata S, Lombardi A, Grimaldi A, Caputo C, Crispo A, Celentano E, De Feo G, Circelli L, Savarese G, Ruggiero R, Perri F, Granata V, Botti G, Caraglia M, Nasti G, and Muto P
- Abstract
Background: in recent years, the management of advanced colorectal cancer (CRC) has been greatly improved with integrated strategies including stereotactic radiation therapy (SRT). The administration of SRT has been demonstrated, particularly in oligo-metastatic (om) CRC, to be a safe and effective option. Interestingly, it has been demonstrated that SRT can induce regression of tumors in non-irradiated regions ("abscopal effect") through stimulation of anti-tumor immune effects ("radiation-induced immunity"). We have recently shown that lung-limited omCRC is characterized by regression of tumor clones bearing specific key driver gene mutations., Aims: to assess the genetic evolution on tumor cancer cells induced by SRT in lung-limited omCRC. Secondary objectives included descriptions of the abscopal effect, responses' duration, toxicity, and progression-free survival. A translational research will be performed to evaluate tumor genetic evolution (through liquid biopsies and Next Generation Sequencing), HLA class I repertoire, peripheral immune cells, and cytokine dynamics., Methods: PRELUDE-1 is a prospective translational study. SRT will be administered only to the largest nodule (with a maximum diameter ≤ 25 mm) in omCRC with two or three radiologically evident lesions. The sample size is based on the innovative hypothesis that radiation-induced immunity could induce regression of tumor clones bearing KRAS oncogene mutations. According to the binomial test, considering the frequency of KRAS mutations and assuming a probability of mutant KRAS→wild type KRAS of p
0 = 0.0077, with α = 0.05 and 1-β = 0.60, the final sample size is 25 patients.- Published
- 2021
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47. Acute Toxicity in Hypofractionated/Stereotactic Prostate Radiotherapy of Elderly Patients: Use of the Image-guided Radio Therapy (IGRT) Clarity System.
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DI Franco R, Borzillo V, Alberti D, Ametrano G, Petito A, Coppolaro A, Tarantino I, Rossetti S, Pignata S, Iovane G, Perdonà S, Quarto G, Grimaldi G, Izzo A, Castaldo L, Muscariello R, Serra M, Facchini G, and Muto P
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- Aged, Humans, Male, Radiation Dose Hypofractionation, Ultrasonography, Prostatic Neoplasms radiotherapy, Radiotherapy, Image-Guided adverse effects
- Abstract
Background: The use of intra-fractional monitoring and correction of prostate position with the Image Guided Radio Therapy (IGRT) system can increase the spatial accuracy of dose delivery. Clarity is a system used for intrafraction prostate-motion management, it provides a real-time visualization of prostate with a transperineal ultrasound. The aim of this study was to evaluate the use of Clarity-IGRT on proper intrafraction alignment and monitoring, its impact on Planning Tumor Volume margin and on urinary and rectal toxicity in elderly patients not eligible for surgery., Patients and Methods: Twenty-five elderly prostate cancer patients, median age=75 years (range=75-90 years) were treated with Volumetric Radiotherapy and Clarity-IGRT using 3 different schemes: A) 64.5/72 Gray (Gy) in 30 fractions on prostate and seminal vesicles (6 patients); B) 35 Gy in 5 fractions on prostate and seminal vesicles (12 patients); C): 35 Gy in 5 fractions on prostate (7 patients). Ultrasound identification of the overlapped structures to the detected ones during simulation has been used in each session. A specific software calculates direction and entity of necessary shift to obtain the perfect match. The average misalignment in the three-dimensional space has been determined and shown in a box-plot., Results: All patients completed treatment with mild-moderate toxicity. During treatment, genitourinary toxicity was 32% Grade 1; 4% Grade 2, rectal was 4% Grade 1. At follow-up of 3 months, genitourinary toxicity was 20% Grade 1; 4% Grade 2, rectal toxicity was 4% Grade 2. At follow-up of 6 months, genitourinary toxicity was 4% Grade 1; 4% Grade 2. Rectal toxicity was 4% Grade 2., Conclusion: Radiotherapy with the Clarity System allows a reduction of PTV margins, the amount of fractions can be reduced increasing the total dose, not exacerbating urinary and rectal toxicity with greater patient's compliance., (Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
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- 2021
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48. Dosimetric comparison among cyberknife, helical tomotherapy and VMAT for hypofractionated treatment in localized prostate cancer.
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Serra M, Ametrano G, Borzillo V, Quarto M, Muto M, Di Franco R, Federica S, Loffredo F, and Paolo M
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- Humans, Magnetic Resonance Imaging, Male, Prostate diagnostic imaging, Prostate pathology, Prostatic Neoplasms diagnostic imaging, Prostatic Neoplasms pathology, Radiometry methods, Prostatic Neoplasms radiotherapy, Radiation Dose Hypofractionation, Radiosurgery methods, Radiotherapy, Intensity-Modulated
- Abstract
Hypofractionation for localized prostate cancer treatment is rapidly spreading in the medical community and it is supported by radiobiological evidences (lower α/β ratio compared with surrounding tissues). Stereotactic body radiation therapy (SBRT) is a technique to administer high doses with great precision, which is commonly performed with CyberKnife (CK) in prostate cancer treatment. Since the CyberKnife (CK) is not available at all radiotherapy center, alternative SBRT techniques are available such as Volumetric Modulated Arc Therapy (VMAT) and Helical Tomotherapy (HT). The aim of the present study was to compare the dosimetric differences between the CK, VMAT, and HT plans for localized prostate cancer treatment.Seventeenpatients have been recruited and replanned using VMAT and HT to this purpose: they received the treatment using the CK with a prescription of 36.25 Gy in 5 fractions; bladder, rectum and penis bulb were considered as organs at risk (OAR). In order to compare the techniques, we considered DVHs, PTV coverage, Conformity Index and new Conformity Index, Homogeneity Index, beam-on time and OARs received dose.The 3 treatments methods showed a comparable coverage of the lesion (PTV 95%: 99.8 ± 0.4% CK; 98.5 ± 0.8% VMAT; 99.4 ± 0.5% HT. P < .05) and good sparing of OARs. Nevertheless, the beam-on time showed a significant difference (37 ± 9 m CK; 7.1 ± 0.3 m VMAT; 17 ± 2 m HT. P < .05).Our results showed that, although CK is the best SBRT technique for prostate cancer treatment, in case this technology is not available, it can be replaced by a similar treatment delivered by VMAT technique. VMAT can be administrated only if it has an appropriate Image Guided Radiation Therapy (IGRT) tracking system.
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- 2020
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49. Automatic genetic planning for volumetric modulated arc therapy: A large multi-centre validation for prostate cancer.
- Author
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Fiandra C, Rossi L, Alparone A, Zara S, Vecchi C, Sardo A, Bartoncini S, Loi G, Pisani C, Gino E, Ruo Redda MG, Marco Deotto G, Tini P, Comi S, Zerini D, Ametrano G, Borzillo V, Strigari L, Strolin S, Savini A, Romeo A, Reccanello S, Rumeileh IA, Ciscognetti N, Guerrisi F, Balestra G, Ricardi U, and Heijmen B
- Subjects
- Humans, Male, Organs at Risk, Radiotherapy Dosage, Radiotherapy Planning, Computer-Assisted, Prostatic Neoplasms radiotherapy, Radiotherapy, Intensity-Modulated
- Abstract
Purpose: The first clinical genetic autoplanning algorithm (Genetic Planning Solution, GPS) was validated in ten radiotherapy centres for prostate cancer VMAT by comparison with manual planning (Manual)., Methods: Although there were large differences among centres in planning protocol, GPS was tuned with the data of a single centre and then applied everywhere without any centre-specific fine-tuning. For each centre, ten Manual plans were compared with autoGPS plans, considering dosimetric plan parameters and the Clinical Blind Score (CBS) resulting from blind clinician plan comparisons. AutoGPS plans were used as is, i.e. there was no patient-specific fine-tuning., Results: For nine centres, all ten plans were clinically acceptable. In the remaining centre, only one plan was acceptable. For the 91% acceptable plans, differences between Manual and AutoGPS in target coverage were negligible. OAR doses were significantly lower in AutoGPS plans (p < 0.05); rectum D
15% and Dmean were reduced by 8.1% and 17.9%, bladder D25% and Dmean by 5.9% and 10.3%. According to clinicians, 69% of the acceptable AutoGPS plans were superior to the corresponding Manual plan. In case of preferred Manual plans (31%), perceived advantages compared to autoGPS were minor. QA measurements demonstrated that autoGPS plans were deliverable. A quick configuration adjustment in the centre with unacceptable plans rendered 100% of plans acceptable., Conclusion: A novel, clinically applied genetic autoplanning algorithm was validated in 10 centres for in total 100 prostate cancer patients. High quality plans could be generated at different centres without centre-specific algorithm tuning., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
50. Lithium chloride increases sensitivity to photon irradiation treatment in primary mesenchymal colon cancer cells.
- Author
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Cammarota F, Conte A, Aversano A, Muto P, Ametrano G, Riccio P, Turano M, Valente V, Delrio P, Izzo P, Pierantoni GM, and De Rosa M
- Subjects
- Apoptosis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Cell Proliferation radiation effects, Cell Survival drug effects, Colonic Neoplasms diagnosis, Colonic Neoplasms drug therapy, Colonic Neoplasms radiotherapy, Humans, Radiotherapy, High-Energy methods, Signal Transduction drug effects, Lithium Chloride pharmacology, Photons, Radiation Tolerance drug effects, Radiation-Sensitizing Agents pharmacology
- Abstract
Colorectal cancer (CRC) is the third most prevalent type of cancer worldwide. It is also the second most common cause of cancer‑associated mortality; it accounted for about 9.2% of all cancer deaths in 2018, most of which were due to resistance to therapy. The main treatment for CRC is surgery, generally associated with chemotherapy, radiation therapy and combination therapy. However, while chemo‑radiotherapy kills differentiated cancer cells, mesenchymal stem‑like cells are resistant to this treatment, and this can give rise to therapy‑resistant tumors. Our previous study isolated T88 primary colon cancer cells from a patient with sporadic colon cancer. These cells exhibited mesenchymal and epithelial features, high levels of epithelial‑to‑mesenchymal transition transcription factors, and stemness markers. In addition, it was revealed that lithium chloride (LiCl), a specific glycogen synthase kinase (GSK)‑3β inhibitor, induced both the mesenchymal‑to‑epithelial transition and differentiation, and also reduced cell migration, stemness features and cell plasticity in these primary colon cancer cells. The aim of the present study was to investigate the effect of LiCl treatment on the viability of primary colon cancer cells exposed to 7 Gy delivered by high‑energy photon beams, which corresponds to 6 megavolts of energy. To achieve this aim, the viability of irradiated T88 cells was compared with that of irradiated T88 cells pre‑treated with LiCl. As expected, it was observed that LiCl sensitized primary colon cancer cells to high‑energy photon irradiation treatment. Notably, the decrease in cell viability was greater with combined therapy than with irradiation alone. To explore the molecular basis of this response, the effect of LiCl on the expression of Bax, p53 and Survivin, which are proteins involved in the apoptotic mechanism and in death escape, was analyzed. The present study revealed that LiCl upregulated the expression of pro‑apoptotic proteins and downregulated the expression of proteins involved in survival. These effects were enhanced by high‑energy photon irradiation, suggesting that LiCl could be used to sensitize colon cancer cells to radiation therapy.
- Published
- 2020
- Full Text
- View/download PDF
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