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The present work studied the anticancer effects of four honey varieties on human cells by studying some physical parameters, hydrogen peroxide content, catalase, glucose oxidase, and microbial activities. Honey samples were (nigella sativa, moringa, sidr, pumpkin), which were coded as NS, MO, SI, and PU honey, respectively. The tested bacterial isolates Staphylococcus aureus, and Enterococcus faecalis were used for microbial activities. Two types of human cells, breast, and colon adenocarcinoma cell lines were used for anticancer properties. SI Honey samples showed the lowest value for electrical conductivity 3.19 μS cm−1. PU Honey samples recorded the highest value for hydrogen peroxide contents 1582.05 μM, while SI Honey reported a similar trend of 1501.51 μM. The values of catalase enzyme activity ranged from 2.78 U/g in SI Honey to 3.91 U/g in MO Honey. Glucose oxidase activity ranged from 4.12 U/g in NS Honey samples to 10.55 U/g in MO Honey. The highest antibacterial activity was detected by SI Honey with a zone of inhibition value of 14.99 mm against Staphylococcus aureus. PU Honey was the best to inhibit the microbial loud of Enterococcus faecalis 7.93 mm. SI Honey was the most effective against breast adenocarcinoma cells (IC50 66.15 μg/mL), on the same trend MO Honey was the most effective against colon adenocarcinoma cells (IC50 45.13 μg/mL). While NS Honey recorded lower efficiency against both breast and colon adenocarcinoma cancer cells IC50 95.13 μg/mL and IC50 87.66 μg/mL, respectively. The control detected the lowest cytotoxic effects on both breast and colon adenocarcinoma cancer cells IC50 112.33 μg/mL and IC50 203.97 μg/mL, respectively.

In developing nations, malnutrition is one of the main factors contributing to child mortality. To tackle this threat, the development of grain-based, protein-rich, and nutrient-dense extruded foods has been suggested. In this study, 16 experiments were generated using D-optimal mixture design and, the blending ratios of ingredients for the development of protein rich snack food were optimized. The optimum value for independent variables corresponding to highest desirability (0.775) was recorded as 25% corn, 23.79% rice and 11.21% defatted soy flour. The value of response variables in terms of nutritional quality of developed product viz. protein, fat, carbohydrate and energy content were found to be 20.52%, 1.34%, 55.13% and 337.23 kcal whereas, the sensory quality viz. texture and overall acceptability were obtained as 5.78 N and 7.53, respectively. The product packaged in PET/PET-MET/LDPE was found to be safe for consumption up to six months.

This work was aimed to investigate the physicochemical and microstructural properties of gluten-free noodles with dietary enriched pumpkin flour from canna or arrowroot starch which was enriched with pumpkin flour at different concentrations (0%, 5%, 10%, and 15%). The results showed that gluten-free noodles made from arrowroot starch exhibited higher cooking properties than that prepared from canna starch. The addition of pumpkin flour increased the chemical properties values (ash, protein, dietary fiber, beta-carotene) and the cooking properties values (cooking time, cooking loss, and water absorption). On the other hand, the lightness value, L*, was reduced and a* and b* values are chromaticity coordinates were raised when the percentages of pumpkin flour were increased. The microstructural analysis showed that all noodle samples exhibited no granular structures indicating fully gelatinized starch occurred during the noodle processing. The addition of pumpkin flour increased the small round rough surface particles that appeared on the noodle micro pictures.

Edible coatings and films can help to keep fruits and vegetables fresh and last longer after harvesting. Polyvinyl acetate (PVAc) and shellac coatings had been developed for controlling the postharvest physiological activity of capsicum. Moreover, a comparative effect of polyvinyl acetate and shellac coatings on capsicum was evaluated at refrigerated temperature (10±2 °C) throughout storage. Both coatings were applied directly to the surface of fruit ‘for the investigation of the crucial parameters, such as weight loss, firmness, color, TSS, titratable acidity, pH, chlorophyll and others during storage. Results showed that, as compared to the control (uncoated capsicum), 12.5% of shellac-coated capsicum, and 12.5% of PVAc coating significantly increased firmness retention, reduced weight loss, and improved physicochemical properties. Over the storage, the PVAc coating prevented color change as well as maintained total soluble solids, titratable acidity, and pH. Results also revealed that the PVAc coating showed a better ability to prevent the deterioration of capsicum as compared to the shellac coating during storage. The present study illustrated the comparative effect of PVAc coating over shellac coating on capsicum at refrigerated storage.

This study investigated the microbiological and toxic heavy metals contamination in smoked-dried fish, and evaluates their health risk implications. Fifteen widely consumed fish species were sampled randomly, and their aflatoxins, mercury “Hg,” cadmium “Cd” and lead “Pb” concentrations were evaluated though standard guidelines. The results revealed that fish flesh Hg level ranged from 0.085–0.258 mg/kg, Cd concentration ranged from 0.092–0.204 mg/kg, and Pb level varied from 0.173–0.329 mg/kg; the mean toxic metals level in the freshwater fish tissue was lower, when compared to the levels in the saltwater fish (seafood). Regarding toxic metal toxicity, the average Hg, Cd and Pb estimated daily intake values for children and adult were 0.1200 and 1.63 × 10−5 mg/kg bw/day, 2.13 × 10−4 and 7.57 × 10−5 mg/kg bw/day, and 2.48 × 10−4 and 1.24 × 10−4 mg/kg bw/day respectively. Likewise, children targeted hazard quotients (THQ) values were Hg (0.585), Cd (0.151) and Pb (0.071), while adult THQ values were Hg (0.293), Cd (0.076) and Pb (0.035). Children and adult hazard index (HI) values were 0.949 and 0.404 respectively; while Carcinogenic Risk (CR) values indicated that Cd had higher chances of cancer risks when compared to Pb. Additionally, the aflatoxins level in the fish species ranged from 0.564–9.422 ppb, with the seafood having lower aflatoxins level when compared to the freshwater fish species. Aflatoxins estimated daily intakes (EDI) exposure was 4.81 × 10−3 and 2.41 × 10−3 μg/kg bw/day for the children and adults respectively; while the aflatoxins HI value for children was 7.300 × 10−4, and for adults was 7.293 × 10−4. Health risks assessment of heavy metal accumulation in the fish, for both children and adults portrayed insignificant adverse health effects (THQ < 1), through the oral consumption of the fishes. Aflatoxins level detected in the all fishes was within acceptable limits (10 ppb) as approved by the World Health Organization; however, some of the values for freshwater fishes were above the allowable limit (2 ppb) approved by the European Union. Though, the results depicted a very low risk of toxic metals and aflatoxins poisoning, there is the need for proper evaluation of current fish processing and storage methods to prevent human exposure to toxic metals and aflatoxins introduced through smoked-dried fish consumption.

This study evaluated the impact of anthropogenic factors on the accumulation of toxic materials in wetland. The wetland was micro-zoned into five sub-regions, namely; NDE, ISN, ISS, UGS and BUR. Sediment, water and fishes (catfish and tilapia) were sampled from each locality. Heavy metals (Cd, Cr, Cu, Ni, Fe, Pb and Zn) and total petroleum hydrocarbon (TPH) of the samples were determined. The pollution indices (Bioaccumulation quotient “BQ”, contamination factor “CF”, geoaccumulation index “Igeo”, enrichment factor “EF” and Pollution load index “PLI”) and potential health risks (Metal Pollution Index “MPL”, Estimated Daily Intake “EDI”, Total Health hazard quotient “THQ” and Hazard index “HI”) of the water, sediment and fish flesh were calculated appropriately. The results revealed that the wetland fishes contained viable bacteria which ranged between 1362.0 cfu/g and 2634.3 cfu/g. Heavy metal level ranking in the sediment and water was Fe > Zn > Cu > Pb > Ni > Cr > Cd. Spatially, the heavy metals concentration in the water, sediment and fish followed the order: BUR > UGS > ISN > ISS > NDE. The TPH concentration in water, sediment and fishes differed significantly across the five sub-regions (p≤ 0.05), and its value ranged from 3.44 mg/kg to 99.38 mg/kg for the catfish, 2.08 mg/kg to 83.73 mg/kg for the tilapia, 25.34 mg/kg to 718.87 mg/kg for the sediment and 0.016 mg/kg to 1.099 mg/kg for the water. Spatial ranking of the TPH levels in the fish flesh was BUR > UGS > ISN > NDE > ISS; sediment TPH level ranked BUR > UGS > NDE > ISN > ISS; and the water TPH level ranked BUR > UGS > ISN > ISS > NDE. Human health risks associated with the consumption of the wetland fish ranked thus BUR > UGS > ISN > NDE > ISS. It was evident from the results that anthropogenic actions from resulting poor regional planning had significant effect on environmental health. The results called for environmental protection for public interest in order to prevent disease epidemics.

Composite flour based noodles were developed by optimizing ingredients and processing conditions using Box-Behnken Design. For ingredients optimization, wheat flour (20–25%), refined wheat flour (Maida) (40–50%), defatted soy flour (10–18%) and vegetable powder (5–7%) were taken as independent variables. For process optimization, feed moisture content (30–40%, db), steaming time (7–12 min), drying temperature (45–65 °C) and drying time (6–8 h) were selected as input parameters. The optimum values of ingredients were found to be 20%, 48.54%, 17.39% and 6.52% for wheat, refined wheat, defatted soy flour and vegetable powder, respectively. The optimum value for process variables were recorded as 30.09%, 7.89 min, 64.75 °C and 7.95 h for feed moisture content, steaming time, drying temperature and drying time, respectively. The optimized composite flour and developed noodle showed higher protein content (16.28%), energy value (309.78 kcal), hardness (143.09 g) with low fat (0.972%) and moisture content (4.35%).

The impact of yellow pumpkin powder (YP) substitution (5, 10, and 15%) on wheat flour's physico-functional, pasting, gel texture, and dough rheology was studied. Moreover, the nutritional, organoleptic properties and bioactivity of the composite pan and pita bread were evaluated. An improved water holding capacity was noticed for the blended flour than for control. The pasting parameters were declined significantly (p 0.05) with increasing YP. Composite flours presented a softer gel texture in the presence of YP. Reduced water absorption, increased dough development time, and lower stability for combined flour dough. The bread with YP depicted increased protein, fat, fiber, and mineral contents, while a reduced volume and specific volume were noticed for pan bread. YP incorporated 5% and did not compromise pan and pita bread's color and overall acceptability. Additionally, composite bread depicted higher total phenolics with enhanced antioxidant activities at the higher substitution of YP.

Onion (Allium cepa L.) is the most widely cultivated species of the Allium genus. Onion is rich in many vital nutrients and sulfur (S)-containing several compounds important for human health. Recently, Allium species requirements have gained special attention in this special time due to its export importance. The aim of this study is to compare the effects of the recommended dose of potassium (100% KRD) against three lower levels of potassium mixed with Bacillus circulans, potassium solubilizing bacteria (80% KRD+biofertilizer, 60% KRD+biofertilizer or 40% KRD+ biofertilizer, as well as foliar applications of copper (Cu), boron (B) or putrescine (Put) that have great impacts on yield, quality and onion attributes.The highest level of potassium (100% KRD) resulted in the highest growth parameters and the richest leaves content in N, P, K, S, B, Cu, and carbohydrates, yield, as well as the best quality parameters of bulbs. All growth and yield parameters decreased gradually with reducing the levels of K fertilization. Foliar spraying of Cu, B, and Put combined with all different levels of K fertilizer improved all plant parameters. The highest impact on plant growth was due to Put followed by B at all levels of soil fertilization. Plants that were exogenously sprayed with Put and B exhibited an increase in N, P, K, B, and carbohydrates under different levels of fertilizers, while foliar application with Cu highly increased S and Cu in onion leaves with all levels of K fertilization. The combined treatment of B and all different levels of K fertilizer considerably improved all yield measurements, except with allyl propyl disulfide, which estimated a highly significant increase with Put foliar application coupled with 40% KRD+Bio. In a nutshell, exogenous Put, B, and Cu foliar applications on onion plants increased bulb yield and its quality attributes with the recommended level of potassium or lower recommended levels of K mixed with biofertilizer.

This work aimed to evaluate pinching and paclobutrazol effects on pottedTecoma capensis(Thunb.) Lindl plant growth, flowering, and chemical composition. Assessed the growth, flowering, and chemical characteristics ofTecoma capensistransplants treated with and without pinching and paclobutrazol foliar spray (0, 75, 150, and 200 mg/l). Pinching and paclobutrazol treatments, and particularly the combined treatment of 200 mg/l paclobutrazol with pinching, reduced plant height and improved branch growth and root parameters. After 150 days, leaf midrib anatomy measurements increased significantly with the application of 200 mg/l paclobutrazol and pinching compared to the controls. The highest flower numbers, flower fresh and dry weight, and show value were achieved with the combination treatment of pinching and 200 mg/l paclobutrazoL. In addition, leaf nitrogen, Phosphorus, potassium, total carbohydrates, total chlorophyll, and leaf phenol contents were statistically increased by the interaction between pinching and 200 mg/l paclobutrazol. Moreover, applications of paclobutrazol at 150 mg/l with pinching and 200 mg/l without pinching significantly increased all aforementioned parameters. Pinching and paclobutrazol treatments were effective for achieving desired growth patterns inTecoma capensisplants, particularly with paclobutrazol concentrations of 200 mg/l.

Plants are considered an inestimable source of therapy, and one of the oldest. The structural elucidation of phytocompounds allowed the scientific community to elaborate on new effective drugs, which has considerably enriched the international pharmacopoeia and allowed society to overcome several epidemic and pandemic situations.Centaureaspecies demonstrated over the years a remarkable capacity to treat several illnesses related to inflammation, oxidative stress and even cancer. This study aimed to evaluate the wound healing capacity of the n-butanol (n-BuOH) extract ofCentaurea tougourensisusing a formulation approach, during a period of 18 days. Male Swiss Albino mice were used for this purpose and the wound area was calculated using Image J software. In addition, macroscopic and histopathological analyses were performed. The study revealed a considerable healing process for the two tested concentrations (200 and 400 mg/kg) of the plant extract, reaching at the end of treatment a percentage reduction in wound area of (91.82%) and (99.80%) respectively, noting that no sign of inflammation was detected in the group of mice treated by plant extract. This study demonstrated once again the pharmacological properties of this species.

Water pollution had become a major problem due to its’ negative impact on the human health. Effects of humaninduced actions on groundwater quality were examined in this study. The physicochemical, heavy metals and microbial parameters of groundwater, sampled during the two major climatic periods in Nigeria, were measured according to APHA approved procedures. Results obtained from laboratory tests revealed that anthropogenic activities had substantial effect on the groundwater quality. The groundwater TDS, nitrate, BOD, chloride and phosphate concentrations varied from 23.93 to 42.32 mg/L, 0.54 to 2.16 mg/L, 2.23 to 4.72 mg/L, 10.78 to 19.15 mg/L, and 0.22 to 0.36 mg/L respectively. Likewise, Cd concentration fluctuated between 0 and 0.001 mg/L, Cu varied between 0 and 0.149 mg/L, Fe varied between 0 and 0.293 mg/L, Pb varied between 0 to 0.105 mg/mL, Zn varied between from 0 and 0.768 mg/L, while Ni fluctuated between 0 and 0.001 mg/L. The findings revealed that areas with poor sanitary situations had poor groundwater quality, compared to the areas with improved sanitary situations. Regarding the microbial population, the highest Total Bacteria and Fungi Counts recorded in the groundwater were 1.11×102 cfu/mL and 1.23×102 cfu/mL respectively. Similarly, the highest recorded Enterobacterial spp., Staphylococus arurius, E. coli, Proteus spp. and Shegeela spp. populations were 26.22×102 cfu/mL, 1.23×102 cfu/mL, 0.41 MPN/100 mL, 0.12 cfu/ml and 0.30×102 cfu/mL respectively. Although, the groundwater physicochemical parameters and heavy metals concentrations were within safe drinking water limits; the groundwater was largely contaminated with pathogenic microorganisms, mostly during the rainy season.

The pectinase enzymes are involved in several industrial applications, and industrial waste is one of the largest environmental pollutants, so this study aims to Endo-polygalacturonase (endo-PG) producing using Aspergillus niger AUMC 4156, Penicillium oxalicum AUMC 4153 and P. variotii AUMC 4149 by using some agro-industrial wastes (dried orange peel and sugar beet pulp) as a sole raw carbon source for degradation these waste in the process of urban wastes disposal. The fermentation process was carried out as a submerged culture technique under both shaken and static culture conditions. A. niger AUMC 4156 was the most promising producer of endo-PG under static conditions while P. oxalicum AUMC 4153 was the highest producer of endo-PG under shaken conditions. Sugar beet pulp proved to be the most preferable to orange peel as the only source of carbon in both shaken and static cultures. The medium that encompassing orange peel as a single carbon source afforded the highest protein content with all tested fungal strains in stirred and static cultures in comparison with sugar beet pulp. The highest activity of endo-polygalacuronase that produced using A. niger AUMC 4156 and P. oxalicum AUMC 4153 was achieved by using sugar beet pulp at 3% concentration under static cultures, meanwhile maximal enzyme activity produced by both fungal strains required 2% sugar beet pulp under shaken cultures. Sugar beet pulp showed promised potential as a good inducer for endo-polygalacturoase production, and enzymes production depended on fungal strains, culture medium, and submerged fermentation conditions.

This study focused on the quality, chemical composition, microbiological population, and sensory assessments of stored mackerel fish products such as bouillon and sauce during the cold storage (4 °C) condition throughout the 12 month of storage period. The fish contains appreciable amounts of moisture content (71.33 g/100 g), protein content (22.55 g/100 g) and fat content (4.60 g/100 g), whereas the ash content and carbohydrate was recorded 1.23 g/100 g, and 0.29 g/100 g respectively. The results of the present study showed that the proximate chemical compositions of storage of both products generally increased at the end of the storage period to reach 10.05 g/100 g for protein content, 61.29 g/100 g for carbohydrate, and 17.01 g/100 g for ash content in stock powder. Besides, the sauce has detected an increase in protein content (4.05 g/100 g), fat content (21.12 g/100 g), ash content (3.41 g/100 g) and carbohydrate (15.09 g/100 g) respectively. The counts of Fecal coliform bacteria and Staphylococcus aureus were less than 10 indicating the good hygienic quality of the developed products. The total aerobic plate counts were significantly (p < 0005) higher in mackerel fish sauce (3.0×102 CFU/g) compared to stock powder (1.5×102 CFU/g). Yeast counts were found higher in mackerel sauce (1.9×102 CFU/g) compared to mackerel stock powder (1.2×102 CFU/g) at the end of storage period. The sensory score was slightly reduced after the storage period. In mackerel fish sauce, the long storage period had adversely affected the appearance (8.25), color (8.75) and odor (8.45) of the product, whereas, it enhanced the taste 9.00, and did not affect the texture (8.75) and overall acceptability (8.70). Overall, scores of all sensory attributes of both products were high indicating the acceptability of these products by panellists. The date showed the benefits mackerel fish products and their availability after storage for 12 months.

Air Frying technology is an innovative technique which could produce healthy fried food materials using hot air. The current work aimed to model the kinetic changes in mass transfer, texture and colour attributes and determining the temperature dependency of the rate constants of the mass transfer, texture and colour traits of the air-fried falafel during the air frying process. To achieve these objectives, five mathematical equations were applied to forecast the mass transfer through frying process. The findings of this study indicated that the air-fried falafel moisture content declined as the frying time increases at varied frying temperatures. The outputs of nonlinear regression analysis showed that the moisture loss of air-fried falafel could be satisfactorily fitted to the modified Page model. The outcomes discovered that the alterations in the texture and the total colour difference (ΔE) of air-fried falafel throughout frying process have linearly increased at varied temperatures and were properly fitted to the zero-order kinetic equation. Meanwhile, temperature dependence of the rate constant of moisture loss, texture and colour change was sufficiently described by the Arrhenius-type model. The increase in rate constants was detected because of the growth of frying temperature. The activation energy values of mass transfer, hardness and ΔE were 70, 21.8 and 0.167 kJ/mol, respectively. The information on kinetic modelling the quality changes during air frying of falafel could be beneficial for helping air fried falafel manufactures with the optimum conditions and quality as well.

Ripe date fruits contain phenolic compounds which possess a high antioxidant activity. The current review was carried out to evaluate total phenolic content in ripe date fruits. Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) was followed during the review process. Relevant studies published from inception up to March 2019 were retrieved from three databases. Study selection was performed based on specific inclusion criteria. A total of twenty-two articles were selected and included in the present review. Data collected from these studies were organized, pooled, and analyzed using descriptive statistics. Total phenolic content means and medians have been reported for the collected ripe date fruit samples for each included study and pooled data. The results suggested that ripe date fruits contain a potent total phenolic content that can contribute mainly to their antioxidant properties.

Fish quality is important in the food industry. Studies on the nutritional, microbial and minerals in Indian mackerel fish are limited. Therefore, this study was carried out to assess the quality and production of fish products (balls and fingers). Additionally, the effect of frozen storage for six months on the microbial, nutritional and sensory evaluation of fish balls and fingers was studied. The obtained results showed that the estimated minerals (zinc, cadmium, chromium, copper, lead and mercury) contents in Indian mackerel muscles were lower than the maximum permissible limits for human consumption. The levels of total bacterial counts and total yeast counts in Indian mackerel purchased from three different stores varied. After 6 months of storage, the microbial content decreased in Indian mackerel fish balls and fingers to less than 2.0×102 CFU/g, which was due to the effect of freezing on the growth and activity of microorganisms, while the carbohydrate, fat and energy contents increased, and the ash, protein and moisture contents decreased; however, Indian mackerel fish fingers had elevated ash, carbohydrate, fat, protein and energy contents and a reduced moisture content after freezing. Sensory evaluation of Indian mackerel balls and fingers at the start and end of the storage period (6 months) revealed good scores for appearance, odor, texture, taste and acceptability. These results provide insights into the benefits of good-quality Indian mackerel fish in the fish product industry and their availability after storage for six months.

The reprogramming of energy metabolism is one of the hallmarks of cancer and is crucial for tumor progression. Altered aerobic glycolysis is a well-known characteristic of cancer cell metabolism. In the present study, the expression profiles of key metabolic genes (HK2, PFKM, and PKM2) were assessed in the breast cancer cohort of Pakistan using quantitative polymerase chain reaction (qPCR) and IHC. Expression patterns were correlated with molecular subtypes and clinical parameters in the patients. A significant upregulation of key glycolytic genes was observed in tumor samples in comparison to their adjacent controls (p < 0.0001). The expression of the studied glycolytic genes was significantly increased in late clinical stages, positive nodal involvement, and distant metastasis (p < 0.05). HK2 and PKM2 were found to be upregulated in luminal B, whereas PFKM was overexpressed in the luminal A subtype of breast cancer. The genes were positively correlated with the proliferation marker Ki67 (p < 0.001). Moreover, moderate positive linear correlations between HK2 and PKM2 (r = 0.476), HK2 and PFKM (r = 0.473), and PKM2 and PFKM (r = 0.501) were also observed (p < 0.01). These findings validate that the key regulatory genes in glycolysis can serve as potential biomarkers and/or molecular targets for breast cancer management. However, the clinical significance of these molecules needs to be further validated through in vitro and in vivo experiments.

The reprogramming of energy metabolism is one of the hallmarks of cancer and is crucial for tumor progression. Altered aerobic glycolysis is a well-known characteristic of cancer cell metabolism. In the present study, the expression profiles of key metabolic genes (

Citrox has natural antibacterial effect against many foodborne pathogens and contaminated bacteria. It mainly contains citric acid, ascorbic acid, and malic acid. We studied the antimicrobial effects of citrox solution (1% and 2%) on the total viable count of methicillin-resistant Staphylococcus aureus (MRSA) on chicken meat fillet. The samples were treated with 106 CFU/g of meat, vacuum-packed (VP), and 4 °C for 3, 6, 9, 12, 15, 18, 21 days. We have also studied the effect of citrox on the total volatile basic nitrogen (TVBN) content and pH changes during the storage periods. The results revealed that citrox inhibited the growth of methicillin-resistant S. aureus (MRSA), in the chicken fillet. The total viable count of MRSA gradually decreased in all treated samples that were stored at 4 °C about 2 log cycle than Chicken-Staph groups after 3, 6, 9, and 12 days and about 1 log cycle than Chicken-Salt group after 3, 6, and 9 days of storage. Total volatile base nitrogen (TVB-N) were observed to be reduced in Chicken- Citrox-treated samples stored at 4 °C than Chicken-Staph, and Chicken-Salt treated samples. The results indicated that citrox is effective to reduce the total count of S. aureus (MRSA) in the first three days of storage by a reduction in the number of bacteria 1 log cycle.

Fish and fish products of the most available protein sources for human beings. Indian mackerel is the most available and economic fish sold in markets in different countries and is included in many local dishes. Indian mackerel are characterized by dark muscles because of the high contents of myoglobin and lipids. This study aims to investigate the chemical composition, saturated and unsaturated fatty acid contents, and fatty acid fractions in Indian mackerel muscle. Indian mackerel (R. kanagurta) were purchased from the central fish market in Jizan, which is caught from the port of Jizan, Saudi Arabia. The samples were analyzed to estimate the proximate chemical composition, Lipid Fractions, and Fatty acid analyses from Indian mackerel. In this study, revealed that moisture, protein, and fat are the main constituents of Indian mackerel. The Indian mackerel contained high and comparable levels of saturated fatty acids and unsaturated fatty acids, and the different samples exhibited somewhat similar fatty acid contents. Among unsaturated fatty acids, the content of polyunsaturated fatty acids was higher than that of monounsaturated fatty acids, and those with the highest levels in Indian mackerel were palmitoleic, palmitic, myristic, oleic, 15-tetracosenoic, linolenic, and stearic acids in addition to docosanoic, eicosanoic, 5,8,11,14,17-eicosapentanoic, and 11-eicosenoic pentadecanoic acids. We conclude Indian mackerel is a rich source of protein and lipids. Polyunsaturated fatty acids are the main constituents of lipids, which exert positive effects on the body by providing the main nutrients and preventing disease in the consumer.

In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.

Antibiotic- and heat-resistant bacteria in camel milk is a potential public health problem. Staphylococcus aureus (S. aureus) is an opportunistic pathogen in humans, dairy cattle and camels. We characterized the phenotype and genotype of methicillin-resistant staphylococcal strains recovered from pasteurized and raw camel milk (as control) distributed in the retail markets of Saudi Arabia. Of the 100 samples assessed between March and May 2016, 20 S. aureus isolates were recovered from pasteurized milk, 10 of which were resistant to cefoxitin, and as such, were methicillin-resistant. However, raw camel milk did not contain methicillin-resistant S. aureus (MRSA). Antimicrobial susceptibility tests showed that the resistance ratio for other antibiotics was 60%. We performed a polymerase chain reaction (PCR) assay using primers for the methicillin-resistant gene mecA and nucleotide sequencing to detect and verify the methicillin-resistant strains. Basic local alignment search tool (BLAST) analysis of the gene sequences showed a 96–100% similarity between the resistant isolates and the S. aureus CS100 strain’s mecA gene. Ten of the methicillin-resistant isolates were heat-resistant and were stable at temperatures up to 85°C for 60 s, and three of these were resistant at 90°C for 60 or 90 s. The mean decimal reduction time (D85-value) was 111 s for the ten isolates. Sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) showed that there was no difference in the total protein profiles for the ten methicillin heat-resistant S. aureus (MHRSA) isolates and for S. aureus ATCC 29737. In conclusion, a relatively high percentage of the tested pasteurized camel milk samples contained S. aureus (20%) and MHRSA (10%).