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Most ageing processes are accelerated due to an increasing amount of endogenic stress from outdoor or indoor activity overload. The essence of aloe vera is formulated in the form of a biocellulose mask. It is one of the solutions to deliver essence within polymeric substances, obtained through the fermentation process with the help of Acetobacter xyllinum. The aim of this study is to enhance anti-ageing activity derived from Aloe vera (L) essence combined with vitamin E, thus in the form of a biocellulose mask that will prolong skin contact and reduce the main cotton sheet mask problem of deforestation, hence its eco-friendly benefit. Material and methods: Twelve volunteers around 25 to 45 years old are subjected to a biocellulose mask containing Aloe vera essence combined with vitamin E for at least 4 weeks to achieve a balanced skin criterion including wrinkles, spots, facial pores, and moisture. Aloe vera and vitamin E constituents within F0; F1, F2; F3, [0%, 0%]; [0%, 1%]; [5%, 0 %] and [5 %, 1 %] as per the following. Results: Results showed that bio-cellulose masks containing 5 % Aloe vera with 1 % of vitamin E essence in F3 provide a beneficial anti-ageing activity rather than other biocellulose mask preparations. Conclusion: Biocellulose mask through the fermentation process can improve skin conditions, the formula combined with the well-known activity of aloe vera as an exfoliating agent and antioxidant vitamin E, it can not only provide skin rejuvenation but also provide anti-ageing activity, Більшість процесів старіння прискорюється через збільшення кількості ендогенного стресу від надмірного навантаження на вулиці чи в приміщенні. Есенція Алое вера була розроблена у формі біоцелюлозної маски. Це одне з рішень для розміщення есенції в полімерні речовини, отримані в процесі ферментації за допомогою Acetobacter xyllinum. Метою цього дослідження є посилення ефекту проти старіння при використанні есенції Алое вера (L) у поєднанні з вітаміном Е у формі біоцелюлозної маски, яка збільшує час контакту зі шкірою. Окрім того, використання такої маски дозволяє позбавитись основної проблеми бавовняної маски, пов’язану з вирубкою лісів, а отже ця форма застосування має ще й екологічні переваги. Матеріали та методи: Дванадцять добровольців віком від 25 до 45 років використовували масці з біоцелюлози, що містить есенцію алое вера в поєднанні з вітаміном Е протягом щонайменше 4 тижнів для досягнення збалансованого стану шкіри, включаючи зморшки, плями, пори на обличчі та зволоження. Алое вера та вітаміни Е входили в склади F0, F1, F2, F3 в наступних співвідношеннях [0%, 0%]; [0%, 1%]; [5%, 0%] і [5%, 1%]. Результати: Результати показали, що маски з біоцелюлози, що містять 5% алое віра з 1% есенції вітаміну Е у F3, мають більш виразний ефект проти старіння, у порівнянні з іншими масками з біоцелюлози. Висновок: Біоцелюлозна маска завдяки процесу ферментації може покращити стан шкіри, формула в поєднанні з добре відомою дією алое віра як відлущувального агента та антиоксидантного вітаміну Е може не лише забезпечити омолодження шкіри, але й забезпечить антивікову дію.

Synergy or additive effect between Aloe vera (L.) Burm.f. and beta-lactam (β-lactam) antibiotics has been reported against Staphylococcus aureus, one of the most important etiological agents of cow mastitis. The goal of the present study was to predict the efficacy of intramammary formulations containing the Aloe vera gel extract in the combination with cloxacillin or ceftiofur at low concentrations in lactating cows as an alternative therapy. Each quarter of 20 healthy Holstein Friesian lactating cows were treated with a single dose of one of the following formulations, corresponding to one of these treatment groups: A1, A2, A3, and A4. A1 and A2 contained cloxacillin at 0.25 and 0.5 mg/ml, whereas A3 and A4 contained ceftiofur 0.25 and 0.5 mg/ml, respectively. In addition, all formulations contained 600 mg/ml of an alcoholic extract of Aloe vera. Milk samples were taken at predefined time points. Antibiotics and aloin (active compound of Aloe vera) concentrations were assessed by liquid chromatography mass spectrometry system (LC-MS/MS). Pharmacokinetic profiles were obtained, and the efficacy index, the fraction of dosing interval in which the antimicrobial concentration remains above the minimum inhibitory concentrations (MICs) (T > MIC) for each formulation, was calculated considering MIC values against Staphylococcus aureus ATCC 29213 as obtained for the combination Aloe vera + antibiotic and aloin concentration in the extract. Mammary gland safety assessment was performed for each combination. Values of the main efficacy index for this study, T > MIC (h) for Aloe vera were 23.29, 10.50, 27.50, and 13.89, whereas for cloxacillin or ceftiofur were 19.20, 10.9, 19.74, and 15.63, for A1, A2, A3, and A4, respectively. Only A1 and A3 reached aloin and antibiotic recommended values as predictors of clinical efficacy for cloxacillin, ceftiofur, and aloin (50, 70, and 60%, respectively), assuming a dose interval of 24 h. The efficacy index values obtained suggest that A1 and A3 might be an effective therapy to treat bovine mastitis caused by S. aureus after a single dose. Nevertheless, further trials in S. aureus mastitis clinical cases are mandatory to confirm the efficacy of Aloe vera formulations.

En los últimos años, la demanda de productos saludables se ha venido incrementando, con lo cual, muchas investigaciones se han focalizado hacia la producción de alimentos y bebidas con potencial nutracéutico. El objetivo de este trabajo fue desarrollar una bebida energizante, a base de panela, jugo de maracuyá, cristales de aloe vera (AV), con propiedades antioxidantes. Se evaluó el contenido de compuestos fenólicos, ácido ascórbico y la capacidad antirradicalaria de las materias primas y las bebidas producidas. Se realizó un análisis sensorial, para verificar la aceptación del sabor, color, textura del AV y la aceptabilidad global de tres bebidas, seleccionadas de acuerdo con sus propiedades antioxidantes. Los resultados mostraron que la panela tenía el mayor contenido de compuestos fenólicos (59,4 ± 0,2mg AGE/g), mientras que el jugo de maracuyá, la mayor actividad antirradicalaria (657 ± 5µg eq AA/mL). Las bebidas analizadas dentro del diseño experimental variaron su actividad antioxidante, con la variación de los factores. De las tres bebidas seleccionadas, la bebida 2 presentó la mayor capacidad antirradicalaria (419 ± 1µg eq AA/mL) y contenido de vitamina C (15,75 ± 0,03µg/mL) y, además, un importante contenido de compuestos fenólicos (7,6 ± 2mg AGE/mL). Asimismo, los resultados del panel sensorial mostraron que la bebida 2 tenía una alta aceptabilidad global y una mayor aceptación del sabor, por lo cual, se puede concluir que esta bebida, es la que presenta mayor potencial antioxidante y comercial. In recent years, the demand for healthy products has been increasing, so much research has focused on the production of foods and beverages with nutraceutical potential. The aim of this work was to develop an energy drink, based on panela, passion fruit juice and aloe vera (AV) crystals with antioxidant properties. The content of phenolic compounds, ascorbic acid and the anti-radical capacity of the raw materials and beverages produced were evaluated. A sensory analysis was performed, to verify the acceptance of taste, color, AV texture and overall acceptability of three beverages, selected according to their antioxidant properties. The results showed that panela had the highest content of phenolic compounds (59.4 ± 0.2mg AGE/g), while passion fruit juice had the highest anti-radical activity (657 ± 5μg eq AA/mL). The beverages analyzed within the experimental design varied in their antioxidant activity with varying factors. Of the three drinks selected, drink 2 had the highest anti-radical capacity (419 ± 1μg AA eq/mL) and vitamin C content (15,75 ± 0,03μg/mL) and also a significant content of phenolic compounds (7,6 ± 2mg AGE/mL). Likewise, the results of the sensory panel showed that beverage 2 had a high overall acceptability and a greater acceptance of the taste, so it can be concluded that this drink is the one with the greatest antioxidant and commercial potential. Incluye referencias bibliográficas

Cilj rada bio utvrditi alelopatski utjecaj vodenih ekstrakata od svježih listova vrste Aloe vera (L.) Burm. f. na klijavost i rast korovnih vrsta. U pokusu u Petrijevim zdjelicama ispitan je utjecaj vodenih ekstrakata u koncentracijama od 2, 4, 6, 8, 10% na korovne vrste crna pomoćnica (Solanum nigrum L. emend. Miller) i Teofrastov mračnjak (Abutilon theophrasti Medik.). U posudama s tlom ispitan je utjecaj vodenog ekstrakta u koncentraciji od 20% na nicanje i rast strjeličaste grbice (Lepidium draba L.). Samo su najviše koncentracije vodenog ekstrakta snizile klijavost sjemena crne pomoćnice, dok je zabilježen pozitivan utjecaj na duljinu izdanka, te svježu i suhu masu. Vodeni ekstrakti djelovali su pozitivno na duljinu izdanka Teofrastovog mračnjaka. Nicanje strjeličaste grbice sniženo je za 52,7%, dok rast klijanaca nije bio pod utjecajem ekstrakta, The aim of the study was to determine the allelopathic effect of water extracts from fresh leaves of Aloe vera (L.) Burm. f. on the germination and growth of weeds. In Petri dish experiment the effect of water extracts in concentrations of 2, 4, 6, 8, 10% on black nightshade (Solanum nigrum L. emend. Miller) and velvetleaf (Abutilon theophrasti Medik.) was tested. In pots with soil the impact of water extract at a concentration of 20% on the emergence and growth of hoary cress (Lepidium draba L.) was evaluated. Only the highest concentrations of the water extract decreased the seed germination of black nightshade, while positive effect on the shoot length, and fresh and dry seedlings weight was recorded. Water extracts had positive effect on shoot length of velvetleaf seedlings. Emergence of hoary cress was reduced by 52.7%, while the growth of seedlings was not affected by the extract.

Esta investigación trata sobre la determinación de germanio en exudado de hojas de zábila, mediante la técnica ana lítica espectrometría de absorción atómica con atomización electrotérmica (ETAAS). El exudado de las hojas de zábila es el líquido amarillo y de sabor amargo que emana de las hojas de la planta cortadas en la base. Es también conocido como zumo o látex. Ha sido utilizado en el tratamiento de diversas afecciones tales como: tratamiento de heridas, infecciones bucales, irritaciones de la piel, otras. El germanio es un oligoelemento que ha sido utilizado como inmunoestimulante y en pacientes con neoplasia. La concentración del germanio presente en el exudado de Aloe vera (L.) Burm.f. fue de 7.42 ± 2.33 μg/g. La metodología empleada fue validada por estudio de recuperación, ubicándose en un valor promedio de 97.43 ± 2.22%. La desviación estándar relativa fue de 2.88%, lo que es un indicativo de la precisión. El método utilizado para la determinación del elemento en cuestión es exacto, preciso y libre de interferencias. The objective of this work was to determine the concentration of germanium in Aloe vera (zábila) from leaves exudated, using the electrothermal atomic absorption spectrometry analytical technic (ETAAS). The exudated is a yellow and bitter liquid, used in wounds healing, skin irritations, mouth infections, etc. The germanium is a trace element used as immunestimulant in patients with tumour. A concentration of 7.42 ± 2.33 μg/g was found. The method for the determination of germanium was validated by recovery studies, a value of 97.43 ± 2.22% was obtained for germanium, and the relative standar deviation (RSD%) was 2.88%. The method was exact, precise and free from interferences.