18 results on '"Alexis Fleming"'
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2. Supplementary Data from The miR–181a–SFRP4 Axis Regulates Wnt Activation to Drive Stemness and Platinum Resistance in Ovarian Cancer
- Author
-
Analisa DiFeo, Michael Kahn, Sergio Marchini, Luca Beltrame, Elmar Nurmemmedov, Arshia Surti, Alexis Fleming, Olga Kovalenko, Peronne Joseph, R. Shae Connor, Sreeja Sekhar, Matthew Knarr, and Anil Belur Nagaraj
- Abstract
Supplemental Table 1: Table of all antibodies used in studies including the company, catalog numbers and dilutions used to perform western blots.
- Published
- 2023
3. Data from The miR–181a–SFRP4 Axis Regulates Wnt Activation to Drive Stemness and Platinum Resistance in Ovarian Cancer
- Author
-
Analisa DiFeo, Michael Kahn, Sergio Marchini, Luca Beltrame, Elmar Nurmemmedov, Arshia Surti, Alexis Fleming, Olga Kovalenko, Peronne Joseph, R. Shae Connor, Sreeja Sekhar, Matthew Knarr, and Anil Belur Nagaraj
- Abstract
Wnt signaling is a major driver of stemness and chemoresistance in ovarian cancer, yet the genetic drivers that stimulate its expression remain largely unknown. Unlike other cancers, mutations in the Wnt pathway are not reported in high-grade serous ovarian cancer (HGSOC). Hence, a key challenge that must be addressed to develop effective targeted therapies is to identify nonmutational drivers of Wnt activation. Using an miRNA sensor-based approach, we have identified miR-181a as a novel driver of Wnt/β-catenin signaling. miR-181ahigh primary HGSOC cells exhibited increased Wnt/β-catenin signaling, which was associated with increased stem-cell frequency and platinum resistance. Consistent with these findings, inhibition of β-catenin decreased stem-like properties in miR-181ahigh cell populations and downregulated miR-181a. The Wnt inhibitor SFRP4 was identified as a novel target of miR-181a. Overall, our results demonstrate that miR-181a is a nonmutational activator of Wnt signaling that drives stemness and chemoresistance in HGSOC, suggesting that the miR–181a–SFRP4 axis can be evaluated as a novel biomarker for β-catenin–targeted therapy in this disease.Significance:These results demonstrate that miR-181a is an activator of Wnt signaling that drives stemness and chemoresistance in HGSOC and may be targeted therapeutically in recurrent disease.
- Published
- 2023
4. Supplemental Figures from The miR–181a–SFRP4 Axis Regulates Wnt Activation to Drive Stemness and Platinum Resistance in Ovarian Cancer
- Author
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Analisa DiFeo, Michael Kahn, Sergio Marchini, Luca Beltrame, Elmar Nurmemmedov, Arshia Surti, Alexis Fleming, Olga Kovalenko, Peronne Joseph, R. Shae Connor, Sreeja Sekhar, Matthew Knarr, and Anil Belur Nagaraj
- Abstract
Supplemental Figures 1-8.
- Published
- 2023
5. Upregulation of DNA repair genes and cell extrusion underpin the remarkable radiation resistance of Trichoplax adhaerens
- Author
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Alexis Fleming, Angelo Fortunato, Carlo C. Maley, and Athena Aktipis
- Subjects
DNA Repair ,Somatic cell ,Cell ,ved/biology.organism_classification_rank.species ,Gene Identification and Analysis ,Gene Expression ,Apoptosis ,Biochemistry ,Radiation Tolerance ,Short Reports ,Gene expression ,Medicine and Health Sciences ,Biology (General) ,Cell Death ,General Neuroscience ,Eukaryota ,Radiation Exposure ,Up-Regulation ,Cell biology ,Nucleic acids ,medicine.anatomical_structure ,Oncology ,Cell Processes ,General Agricultural and Biological Sciences ,DNA damage ,QH301-705.5 ,DNA repair ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Trichoplax ,Trichoplax Adhaerens ,Genetics ,medicine ,Animals ,Placozoa ,Model organism ,Mutation Detection ,Gene ,General Immunology and Microbiology ,Whole Genome Sequencing ,ved/biology ,X-Rays ,Organisms ,Biology and Life Sciences ,Cancers and Neoplasms ,Cell Biology ,DNA ,Sequence Analysis, DNA ,biology.organism_classification ,Invertebrates ,Cellular Extrusion ,Multicellular organism ,Gene Expression Regulation ,Zoology - Abstract
Trichoplax adhaerens is the simplest multicellular animal with tissue differentiation and somatic cell turnover. Like all other multicellular organisms, it should be vulnerable to cancer, yet there have been no reports of cancer in T. adhaerens or any other placozoan. We investigated the cancer resistance of T. adhaerens, discovering that they are able to tolerate high levels of radiation damage (218.6 Gy). To investigate how T. adhaerens survive levels of radiation that are lethal to other animals, we examined gene expression after the X-ray exposure, finding overexpression of genes involved in DNA repair and apoptosis including the MDM2 gene. We also discovered that T. adhaerens extrudes clusters of inviable cells after X-ray exposure. T. adhaerens is a valuable model organism for studying the molecular, genetic, and tissue-level mechanisms underlying cancer suppression., The placozoan Trichoplax adhaerens is able to tolerate high levels of radiation and is resilient to DNA damage; this study reveals that exposure to X-rays triggers the extrusion of cell clusters which subsequently die, and that radiation exposure induces the overexpression of genes involved in DNA repair.
- Published
- 2020
6. The miR-181a-SFRP4 Axis Regulates Wnt Activation to Drive Stemness and Platinum Resistance in Ovarian Cancer
- Author
-
Arshia Surti, Analisa DiFeo, Elmar Nurmemmedov, Anil Belur Nagaraj, Michael Kahn, Alexis Fleming, Luca Beltrame, Sergio Marchini, R. Shae Connor, Peronne Joseph, Sreeja C. Sekhar, Olga Kovalenko, and Matthew Knarr
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,Down-Regulation ,Antineoplastic Agents ,Disease ,Biology ,Article ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Proto-Oncogene Proteins ,microRNA ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Molecular Targeted Therapy ,Wnt Signaling Pathway ,beta Catenin ,Ovarian Neoplasms ,Activator (genetics) ,Wnt signaling pathway ,medicine.disease ,Biomarker (cell) ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Mutation ,Cancer research ,Neoplastic Stem Cells ,Female ,SFRP4 ,Cisplatin ,Neoplasm Grading ,Ovarian cancer - Abstract
Wnt signaling is a major driver of stemness and chemoresistance in ovarian cancer, yet the genetic drivers that stimulate its expression remain largely unknown. Unlike other cancers, mutations in the Wnt pathway are not reported in high-grade serous ovarian cancer (HGSOC). Hence, a key challenge that must be addressed to develop effective targeted therapies is to identify nonmutational drivers of Wnt activation. Using an miRNA sensor-based approach, we have identified miR-181a as a novel driver of Wnt/β-catenin signaling. miR-181ahigh primary HGSOC cells exhibited increased Wnt/β-catenin signaling, which was associated with increased stem-cell frequency and platinum resistance. Consistent with these findings, inhibition of β-catenin decreased stem-like properties in miR-181ahigh cell populations and downregulated miR-181a. The Wnt inhibitor SFRP4 was identified as a novel target of miR-181a. Overall, our results demonstrate that miR-181a is a nonmutational activator of Wnt signaling that drives stemness and chemoresistance in HGSOC, suggesting that the miR–181a–SFRP4 axis can be evaluated as a novel biomarker for β-catenin–targeted therapy in this disease. Significance: These results demonstrate that miR-181a is an activator of Wnt signaling that drives stemness and chemoresistance in HGSOC and may be targeted therapeutically in recurrent disease.
- Published
- 2020
7. Hex and the Single Girl
- Author
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Alexis Fleming and Alexis Fleming
- Subjects
- Demonology--Fiction
- Abstract
FROM EXCITING ROMANCE AUTHOR ALEXIS FLEMING Book three in the Lucifer Inc. series How's a hexy chick gonna get laid if even breathing is an adventure sport? Sabrina—Sabby to her friends—is a hex, a pariah. Even Lucifer crosses the road to avoid her. Everyone avoids her…except the sex-mad humpomaniac imps, that is. Chaos demons are known for…well, chaos. And for Sabrina, chaos equals misery, until she's dragged to Earth by literary historian Jude Morrisey. Human, good-looking and definitely sex on legs, Jude is enough to set her libido on high alert. Now if she can just keep her chaos magic from ripping his life apart, things might be looking up. When Jude accidently summons a demon, his staid bachelor world erupts into an unholy mess. Someone's sabotaging his career and his job's almost down the gurgler. Sex-crazed imps invade his house and hump his appliances. The biggest shock of all is the sexy Calamity Jane who appears in his attic insisting she's his jinx. Harmony, peace and his staid life are out of the attic window, but here's the thing… Can he keep his hands off the delectable walking disaster long enough to work out what's going on? Anarchy, bedlam, chaos… It's all in a day's work for a hexy chick.
- Published
- 2021
8. Demons High!
- Author
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Alexis Fleming and Alexis Fleming
- Subjects
- Interpersonal relations--Fiction, Nightclubs--Fiction
- Abstract
FROM EXCITING ROMANCE ALEXIS FLEMING Book two in the Lucifer Inc! series A sexy half-demon neighbor, rampaging horny imps and a dead aunt who's very much alive… Could life get any more chaotic? When Elli Gavin inherits her aunt's run-down house, the last thing she expects to find is a bunch of Lucifer's imps running a raunchy, imp-humping nightclub and secret poker games in her house every night. Tate Belor is no ordinary man. He's part human, part shadow demon and the head of Lucifer's security detail—and now, thanks to Lucifer, an imp hunter and babysitter. Then he gets his first look at ‘little Elli'. She's a very grown-up woman with the ability to shatter his legendary control over his emotions. Invisible humping imps, a believed-dead-but-very-much-alive aunt and a nasty human out to steal her house… Elli's surrounded by mystery and more questions than answers. What will she do when the truth is discovered? More importantly, can Elli and Tate survive the Hell-storm together? But just remember… The house always wins.
- Published
- 2020
9. Mayhem 'n' Mistletoe
- Author
-
Alexis Fleming and Alexis Fleming
- Subjects
- Romance fiction, Fantasy fiction, Man-woman relationships--Fiction, Erotic stories
- Abstract
WANTED FOR TOO MUCH CHEER: Misty Toll. Dealer of mistletoe, aka Happy Juice. Be extremely careful. Too hot to handle.When Christmas is declared illegal, Misty Toll finds herself dealing mistletoe juice to fund Santa's revolution to bring back Christmas. But Santa's addicted to his own product, Rudolph's red nose is from too much vodka, and there's a bunch of rabid revolutionary elves on the run.Then there's the delicious Scrooge, Zane Mason, the new man hired to work on the deer farm. The same farm where she's hiding Santa's reindeers and her secret stash of mistletoe.What's a girl to do but go with the holiday cheer?
- Published
- 2015
10. Dating for Demons
- Author
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Alexis Fleming and Alexis Fleming
- Abstract
A date with a sexy incubus, horny little imps, kidnapping demons, and an aunt with all the power of the devil… Life is about to get interesting. Araminta Tempest, Mina to her friends, isn't normal by any stretch of the imagination. She's one-eighth succubus demon and her Aunt Lucifer, supreme ruler of Hell, never lets her forget it. Accountant by trade, Mina normally does the books for Aunt Luce's business, but this year Aunt Luce wants her to coach Jedd Madison, stud of the incubi stable and Mina's nemesis from her teenage years, on dating techniques. Mina smells a setup. Maybe it's time to turn the tables on her nosy aunt from Hell.
- Published
- 2014
11. Who's Your Daddy
- Author
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Lyn Cash, Summer Devon, Alexis Fleming, Lyn Cash, Summer Devon, and Alexis Fleming
- Abstract
'Direct Deposit'by Summer Devon Five years ago she and her husband had used him in a strange threesome that turned out to be less about passion than about an infertile couple's desperation. Now Zack Reese spotted Colleen Madison's name on a list of fertility centre clients he's supposed to interview for a story about some sperm donor. His curiosity, and his haunting memory of her glorious naked body, overcomes his reluctance to contact her. Divorced and infertile - yet finally content with her life - Colleen is shaken when the man she'd unwittingly deceived re-enters her life. She can't forget that their one encounter left her wanting more. His re-entry into her life reawakens a passion she thought she'd lost years earlier.'Strategic Withdrawal'by Alexis Fleming WANTED: Sperm Donor #69. Tired of using a test tube? Sick of the sterility of the lab? If so, come join me for two weeks of red-hot lovin.'Satisfaction guaranteed! Mandy Dodds wants a baby before her biological clock implodes. A withdrawal from a donor bank smacks too much of buying a baby for Mandy's sensibilities so she comes up with a plan. Track down Sperm Donor #69, the father of three clients'babies, and do things the old-fashioned way. Travis McCloud seems to be that man. But there's just something about him that doesn't add up. What will Mandy's reaction be when she finds out Travis McCloud has been using her for his own agenda?'Playing the Ace'by Lyn Cash Look at photos of a gorgeous female form with his left hand, become intimate with his Johnson with his right, give the nice nurse his resulting specimen, and walk back to his dorm with a pocketful of cash. Who knew that a few random deposits during his college years would yield such a grand lifetime investment by the time he was in his thirties? Ace discovers how wealthy he is when he meets the blonde bombshell attorney representing two of the children he spawned back when he was known as Sperm Donor #69.
- Published
- 2008
12. Lust Bites: Vol 2
- Author
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Jamie Hill, Alexis Fleming, Lyn Cash Hill, Jamie Hill, Alexis Fleming, and Lyn Cash Hill
- Abstract
Lust Bites Anthology Collection - Volume Two This Lust Bites Collection was released to celebrate TB's first anniversary. A collection of all of the short stories released throughout the year. A collection of all of the short stories released throughout the year'Convincing Cate'by Jamie Hill Vince Mason is sure the perfect woman doesn't exist. When he meets elusive Cate Reynolds and finds she's nearly as close to perfect as he can imagine, he doesn't want her to slip away. Cate is attracted to Vince, but she sees one major problem—he's almost ten years younger, an age span she refuses to live with. It's going to take one carefully crafted plan, a few well-meaning relatives, and maybe even a shot from Cupid's arrow before Vince is through convincing Cate.'Hit and Miss'by Lyn Cash and Alexis Fleming Book one in the Sexy Mythconceptions Series Nothing like a little foray into BDSM to make a girl feel good about herself. When Kat Toomy discovers her lover cavorting on her bed in her underwear with his best buddy, she knows it's time to kick his butt out of there and go looking for something to restore her confidence in herself. Kat's girlfriends offer her a weekend away on a tropical Australian resort that specialises in fulfilling fantasies and she's all for it, particularly when she meets her new Master, Gabe Carpenter, who can turn her on with the flick of a button. But how will Kat and Gabe feel when they find out they've been set up?'How Sweet It Is'by Shermaine Williams As a successful male escort, Ben's life is sweet. He goes to all the best places with a wide range of women who adore him. Granted, not all of them would be his first choice and it's difficult to maintain a serious relationship when your job is to provide pleasure to other women, but that is a small price to pay. He has a lovely home, a wonderful lifestyle and wants for nothing. Maybe it's not what his parents always dreamed of him doing, but he is a professional; that is until Natasha's arrival on the scene. He fails to anticipate that she is capable of doing what no other woman can…putting his professionalism to the test.'Crude Oil'by Desiree Holt Dana Devlin was still recovering from a messy divorce following an even messier marriage. When her sister Jodi sent her to Enchantor Key for a weekend of relaxing and unwinding, the last thing she expected was a Meet Your Mate event. And the last person she expected to see what sexy hunk Max Hollister, a divorce attorney about whom she secretly fantasised. Touch was enough to turn her on, but when he brought out his special oil, she was lost to an erotic adventure.'Fantasies Unleashed'by Skylar Sinclair Sexual fantasies are just that, fantasies, until they are played out in physically, graphic, erotic reality! Ever fantasised about partaking in a night of uninhibited sex with a stranger? Letting him do things to your body that you have only dreamed of? Bella Simmons is looking to fulfil the fantasy she has longed to explore, but up until now, has never dared. Bored with her life she decides enough is enough, and ventures out one night with the clear intent of finding a sexy stranger and having the wildest carnal night of her life.'Tempting Tamera'by Brynn Paulin Book one in the Circle of Three Series A new job, a new home, a new lifestyle… When teacher Tamera moved to Cranston to teach, she knew the lifestyle she'd encounter would take some getting used to. Though she was raised in an open-minded household she doesn't have experience with ménage relationships therefore her attraction to her fellow teachers, Kai and Brian, takes her by surprise. When the trio is stranded together during a freak snowstorm,
- Published
- 2008
13. Hit and Miss
- Author
-
Alexis Fleming, Lyn Cash, Alexis Fleming, and Lyn Cash
- Subjects
- Sexual dominance and submission--Fiction, Bondage (Sexual behavior)--Fiction, Man-woman relationships--Fiction
- Abstract
Nothing like a little foray into BDSM to make a girl feel good about herself.When Kat Toomy discovers her lover cavorting on her bed in her underwear with his best buddy, she knows it's time to kick his butt out of there and go looking for something to restore her confidence in herself. Kat's girlfriends offer her a weekend away on a tropical Australian resort that specialises in fulfilling fantasies and she's all for it, particularly when she meets her new Master, Gabe Carpenter, who can turn her on with the flick of a button. But how will Kat and Gabe feel when they find out they've been set up?This book has both male and female dominance.
- Published
- 2008
14. Pandemonium
- Author
-
Alexis Fleming, Lyn Cash, Alexis Fleming, and Lyn Cash
- Subjects
- Man-woman relationships--Fiction, Erotic stories, Mythology, Classical--Fiction, Women psychologists--Fiction
- Abstract
The love doctor is in...but can he take a dose of his own medicine?Pan, the Arcadian god of lust and magic, has had enough of his restricted existence hidden away on Arcadia. When Cupid, the Greek god of love, suggests he live on Earth, Pan decides to give it a go. Pan establishes himself as the luuuuuv doctor and announcer for a nighttime romantic music slot with a local radio station. He plays his pan-pipes, enthralling all who listen. Cupid warns Pan that his days of playing fast and loose with women's emotions are numbered. Sure enough, Pan meets Kris, the woman who takes him to task. The leather-wearing, Harley-riding, hard-ass psychologist has her own agenda, and it doesn't include him. Before he knows what's happening, she has turned the table on the god, and he's smitten.Kris is exactly the right medication, the perfect dose for the nomadic God of Lust, who has finally found a love of his own.
- Published
- 2008
15. Who's Your Daddy
- Author
-
Alexis Fleming, Lyn Cash, Summer Devon, Alexis Fleming, Lyn Cash, and Summer Devon
- Abstract
'Direct Deposit'by Summer DevonFive years ago she and her husband had used him in a strange threesome that turned out to be less about passion than about an infertile couple's desperation. Now Zack Reese spotted Colleen Madison's name on a list of fertility centre clients he's supposed to interview for a story about some sperm donor. His curiosity, and his haunting memory of her glorious naked body, overcomes his reluctance to contact her.Divorced and infertile - yet finally content with her life - Colleen is shaken when the man she'd unwittingly deceived re-enters her life. She can't forget that their one encounter left her wanting more. His re-entry into her life reawakens a passion she thought she'd lost years earlier.'Strategic Withdrawal'by Alexis FlemingWANTED: Sperm Donor #69. Tired of using a test tube? Sick of the sterility of the lab? If so, come join me for two weeks of red-hot lovin.'Satisfaction guaranteed!Mandy Dodds wants a baby before her biological clock implodes. A withdrawal from a donor bank smacks too much of buying a baby for Mandy's sensibilities so she comes up with a plan. Track down Sperm Donor #69, the father of three clients'babies, and do things the old-fashioned way.Travis McCloud seems to be that man. But there's just something about him that doesn't add up.What will Mandy's reaction be when she finds out Travis McCloud has been using her for his own agenda?'Playing the Ace'by Lyn CashLook at photos of a gorgeous female form with his left hand, become intimate with his Johnson with his right, give the nice nurse his resulting specimen, and walk back to his dorm with a pocketful of cash.Who knew that a few random deposits during his college years would yield such a grand lifetime investment by the time he was in his thirties?Ace discovers how wealthy he is when he meets the blonde bombshell attorney representing two of the children he spawned back when he was known as Sperm Donor #69.
- Published
- 2008
16. Pandemonium
- Author
-
Lyn Cash, Alexis Fleming, Lyn Cash, and Alexis Fleming
- Abstract
The love doctor is in…but can he take a dose of his own medicine? Pan, the Arcadian god of lust and magic, has had enough of his restricted existence hidden away on Arcadia. When Cupid, the Greek god of love, suggests he live on Earth, Pan decides to give it a go. Pan establishes himself as the luuuuuv doctor and announcer for a nighttime romantic music slot with a local radio station. He plays his pan-pipes, enthralling all who listen. Cupid warns Pan that his days of playing fast and loose with women's emotions are numbered. Sure enough, Pan meets Kris, the woman who takes him to task. The leather-wearing, Harley-riding, hard-ass psychologist has her own agenda, and it doesn't include him. Before he knows what's happening, she has turned the table on the god, and he's smitten. Kris is exactly the right medication, the perfect dose for the nomadic God of Lust, who has finally found a love of his own.
- Published
- 2008
17. Hit and Miss
- Author
-
Lyn Cash, Alexis Fleming, Lyn Cash, and Alexis Fleming
- Abstract
Nothing like a little foray into BDSM to make a girl feel good about herself. When Kat Toomy discovers her lover cavorting on her bed in her underwear with his best buddy, she knows it's time to kick his butt out of there and go looking for something to restore her confidence in herself. Kat's girlfriends offer her a weekend away on a tropical Australian resort that specialises in fulfilling fantasies and she's all for it, particularly when she meets her new Master, Gabe Carpenter, who can turn her on with the flick of a button. But how will Kat and Gabe feel when they find out they've been set up? This book has both male and female dominance.
- Published
- 2008
18. Sink or Swim
- Author
-
Alexis Fleming and Alexis Fleming
- Abstract
Do mermaids have orgasms? Who knows, but she'd better find out quick before she exchanges her girly bits for fishy slits. A mermaid who can't swim? What could be worse? When Samie McIntyre finds out she's about to turn into a mermaid, a legacy of her selkie father and mermaid mother, she realises she has one major problem. Well, er, maybe two… First, she can't swim. And second, she has aqua-phobia. What good is a mermaid who's afraid of the water? As far as she can see, there's only one thing to do. Talk the sexy Jonah Billings into teaching her to swim. And if she can convince him to indulge in a bit of horizontal wrestling before she becomes a pickled sardine, so much the better.
- Published
- 2007
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