Search

This study provides the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in blood profile and pathological changes of liver albino in male rats, the aims of present study were detected the influence of TCDD in the male rats, the present study was include (118 albino male rats) divided into three groups, their age ranged from 8-9 week, the LD50 of TCDD (70.7 μg /kg) and the period of this study 90 days. The hematological examination showed increased in WBCs while decreased in RBCs, Hb., platelets in 2nd & 3rd group, the pathological examination revealed in 2nd & 3rd group in liver enlargement and pale in color, the histopathological changes in liver showed extensive necrosis, cirrhosis, dilation in sinusoid, increased cellularity, granulomatous reaction and sever infiltration of inflammatory cells, acute cellular swelling with mild sinusoid and infiltration of neutrophils, moderate infiltration of inflammatory cells, sinusoid dilation with extensive hemorrhage, fibroblast cells aggregation with midzone areas of necrosis. [ABSTRACT FROM AUTHOR]

This study provides the effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in biochemical and pathological changes of kidney albino in male rats, the aims of present study were detected the influence of TCDD in the male rats, the present study was include (118 albino male rats) divided into three groups, their age ranged from 8-9 week, the LD50 of TCDD (70.7 µg/kg) and the period of this study 90 days. The biochemical assay showed decreased in reduced glutathione, Catalase & increased in Malon di-aldehyde in 2nd & 3rd group. The pathological examination revealed in 2nd & 3rd group in kidney acute tubular necrosis of renal tubules characterized by dark acidophilic cytoplasm & destructive nuclei & most epithelium lining were sloughed in lumen, interstitial hemorrhage with mild mononuclear infiltration, severe mononuclear cells interstitial infiltration with degeneration & necrosis of tubules, acidophilic homogenous amorphous irregular granuloma present in the interstitial layer with caseous necrosis & surrounded by foreign body giant cells, mild fibrous tissue capsule & all tubules are degenerated. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the effectiveness of 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD). Rats (n=30) were randomly and equally divided into three groups, 1st group considered control, 2nd group orally administered for 30 day 125 ng/kg/day TCDD, and 3rd group orally administered for 90 day 125 ng/kg/day TCDD respectively by stomach tube, TCDD dissolved in corn oil. Liver samples were taken from half of rats on day 30 and 90 and biochemical assay of fasting serum peroxynitrate radical, TG-HDL, LDL and VLDL cholesterol and serum catalase activity. Results indicated that TCDD significantly (P<0.05) increased in lipid profile and decrease catalase and peroxynitrate radical. Pathological examination showed liver damage, abscess, centrilobular necrosis with massive calcified giant cells (epithelioid and macrophage) extensive granuloma, In conclusion the TCDD showed intoxicant and early carcinogenesis in rats and it's time and dose depended manner. [ABSTRACT FROM AUTHOR]

Dioxins are complex chemical materials occurring as intermediates and by product like plastic waste incineration, also during production process of chlorination in many chemical industries, TCDD is stable chemical structure hydrophilic and low solubility in water, the current experiment examined the effect of TCDD on kidney in guinea pigs, Hartley strain male guinea pigs (200-250 g weight), 42 animal were divided into 2 groups equally. 1st groups received normal pellets while 2nd group (TCDD group) received 0.1µg/kg b.wt. of TCDD weekly for 12 weeks orally by stomach tube. Biochemical analysis of serum (urea, and creatinine mg/dl), glutathione were done at 4, 8 and 12 weeks also WBC count were detected with pathological changes in kidney, current study showed significant increase in urea, creatinine concentration and WBC count, while decrease glutathione concentration in TCDD group specially at 8 and 12 weeks. Pathological changes data characterized by kidney edema, acute cellular swelling, amyloid, hyaline cast, multiple abscess formation and nephritis. [ABSTRACT FROM AUTHOR]

Current experiment was designed to evaluate the chronic effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) intoxicant effects and Curcumin antioxidant effects on pathological and hormonal assay in Sprague Dawley male rats at day 90. Thirty male rats divided randomly and equally into 3 groups, control group (negative control) received only normal pellet and corn oil, 2nd group (positive group) act as TCDD group received orally weekly for 90 day by stomach tube (2 µg/kg), while 3rd group received weekly by stomach tube (2 µg/kg) TCDD with (100 mg/kg) daily Curcumin for 90 day. Testosterone hormone and sperm analysis (viability, number and abnormality) assay significantly decrease (P<0.05) at 2nd group when compare with control and 3rd group, while pathological changes indicated at 2nd group testicular carcinoma characterized by pleomorphic and polyhydral of leydig cells and sertoli cells. The histopathological examination refer to leydig and sertoli cells carcinoma (testis carcinoma). [ABSTRACT FROM AUTHOR]

2,3,7,8 tetra-chlorodibenzo-p-dioxin (TCDD) is a multispecies reproductive toxicant recently by IARC classified human carcinogen, TCDD promot tumorgenesis of ovarian tumor in domestic brown hens, two hundred domestic hens divided equally and randomly into 2 groups: 1st group feeding on commercial feed pellet while 2nd group feed weekly for 6 months commercial chicken pellet contaminated by 175 µg/kg TCDD for 6 months. The WBCs, serum FSH hormone and serum Alpha fetoprotein were done at 3 and 6 months of experiment also the histopathological changes for left and right ovaries were done at 3 and 6 months. At end of experiment, all flocks were sacrificed and ovaries tissue were examined and staining by hematoxylin and eosin stain, also by Masson trichome stain to detect fibrous connective tissue. Results of TCDD hens showed significantly increased in WBCs count, serum AFP level and decreased in serum FSH hormone at 3 and 6 months with tumorigenesis in both ovaries at 6 months characterized by ovoid to oval epithelium nuclei, papillary neoplastic cells with increase thickening of fibrostroma. [ABSTRACT FROM AUTHOR]

Rabbits exposure to P. aeruginosa causes pathological lesions and teratological effects in mothers and embryos, this was observed during the experiment in which forty five mature rabbits of both sex were divided into three groups, first group was inoculated both sex with 0.2 ml of distill water while the other two groups ioculated with 0.2 ml of 2*108 CFU/ml of P. aeruginosa "by using 1ml disposable syringes" and as following, infection of pregnant animals in 2nd group by inoculation intrauterine while the pregnant animals in 3rd group was infected intraperitoneally, then the pregnants in all groups had been dividing in to two subgroups for each group, the first subgroups consist from five pregnant which was euthanased on at the 28th day before delivery while the pregnant females in second subgroups euthanasia occurred at the end of the experiment. Showing different levels of teratological lesion in the embryos with pathological lesion in organs of fetus. [ABSTRACT FROM AUTHOR]

Acrylonitrile (ACN) is highly poisonous compound with formula (CH3H3N) used in plastic solvent and acrylic fiber. This experiment was designed to evaluate the toxicopathological effect of ACN on male dog genitals.for acute (30) day period and chronic period (90)day. Twenty four healthy Beagle male dog aged e" 1 years were randomly divided in to three groups each contain each contain eight dogs: 1st (c) control group feeding for 90 day on normal dog diet, 2nd group (T1) daily intake by stomach tube for 30 day ACN (30 mg/kg.b.w) with normal dog diet, while 3rd group (T2) for (90) day daily intake by stomach tube ACN (30 mg/kg.b. w) with normal dog diet at days 30 and 90 hormonal assay of testosterone and FSH were Dane for all group with semen viability, concentration, PH and abnormality. Pathological changes on male genitalia (prostate, tastes and epididymis) were done on scarified dogs at day 90. T1 and T2 groups statistically significant decreased in testosterone level with increase of FSH hormone in serum mostly at T2 in 90 day while T2 showed statistically increased in semen PH, deformities while viability with concentration decreased. T2 group indicated solid homogenous pale cut testis with microscopically lesion mostly at T2 group characterized by polyhydral cells, distended seminiferous tubules and elongated palisading tumor with thick bands of partially hyalinized seminoma. [ABSTRACT FROM AUTHOR]

Current study was aimed to investigate 1,8-Cineole effectiveness as antioxidant defense against dioxins like compound polychlorinated biphenyls (dl-PCBs) toxicity in hen's liver. Domestic hens (n=45) were divided randomly and equally into 3 equal groups. 1st group (control) was given weekly for 6 months orally by gavage corn oil as carrier and feed on normal chicken pellets, 2nd group were given orally by gavage weekly for 6 months PCBs at dose 2 g/kg weekly dissolved freshly in corn oil and evaporated to nitrogen, 3rd group (PCBs+cineole) and acetone mixed 2 g/kg B. W./weekly PCBs + Cineole 1000mg/kg/weekly for 6 months. Half of liver sample were taken for histopathological examination at end of experiment and the other half liver for biochemical assay. In liver thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and catalase (CAT). Results showed that significant increase P<0.05 in TBARS at 2nd group when compared with control and 3rd group, while CAT and GSH indicated significant P<0.05 decreased at 2nd group compared with control and 3rd group. Histopathological changes in the hen's liver at 6 months revealed no important pathological changes in control and 3rd group, while 2nd group (PCBs) showed necrosis, hetrophiles and mononuclear cell infiltration, severe granulation tissue in hepatocyte, disorganization of liver and liver adenoma. [ABSTRACT FROM AUTHOR]

Diabetes mellitus is associated with importance of rat model. The chronic complication of (D.M) type 2 is coronary artery atherosclerosis caused by reactive oxygen species (ROS) by causing hyperlipidemia. Alpha-lipoic acid (ALA) is prevention complication of diabetes mellitus its potent antioxidant by prevention diabetic atherosclerosis and vascular disease. 30 Sprague-Dawley rats randomly equally divided into 3 groups 1st group (control), 2nd group daily injected for 3 days I/P with (40-45 mg/kg) streptozotocin, 3rd group daily injected I/P with (40-45 mg/kg) streptozotocin for 3 days daily supplement with ALA (300 mg/kg) for 60 day. Glucose concentration and lipid profile increased with concentration of insulin resistance IR and GH-PX mg/Hb/min at 2nd group mostly at 60 days with atherosclerotic plaque in the coronary artery, while 3rd group showed decreased in glucose and lipid profile and decrease IR with GH-Hb at 60 day without pathological atherosclerotic plaque in coronary artery. [ABSTRACT FROM AUTHOR]