22 results on '"Akemi Takamizawa"'
Search Results
2. Hypoxia-sensitive molecules may modulate the development of atherosclerosis in sleep apnoea syndrome
- Author
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Akemi Takamizawa, Keisaku Fujimoto, Motonori Hayashi, Osamu Kinoshita, Kazuhisa Urushibata, and Keishi Kubo
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Male ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Pathology ,Polysomnography ,Severity of Illness Index ,Gastroenterology ,Thromboplastin ,chemistry.chemical_compound ,Tissue factor ,Risk Factors ,Internal medicine ,Severity of illness ,Humans ,Medicine ,HSP70 Heat-Shock Proteins ,Hypoxia ,Analysis of Variance ,Sleep Apnea, Obstructive ,medicine.diagnostic_test ,business.industry ,Case-control study ,Sleep apnea ,Middle Aged ,Hypoxia (medical) ,Atherosclerosis ,medicine.disease ,respiratory tract diseases ,Vascular endothelial growth factor ,C-Reactive Protein ,chemistry ,Case-Control Studies ,Linear Models ,Intercellular Signaling Peptides and Proteins ,Analysis of variance ,medicine.symptom ,business - Abstract
Objectives: Obstructive sleep apnoea hypopnoea syndrome (OSAHS) is associated with increased morbidity and mortality due to cardiovascular disease. In order to examine the association between OSAHS and cardiovascular disease, this study measured hypoxia-inducible and atherosclerosis-associated molecules in the peripheral blood. Methods: In this study peripheral blood was obtained early in the morning from 60 consecutive male patients with OSAHS (AHI ≥10 events/h) and 30 male control subjects without OSAHS (AHI
- Published
- 2006
3. Relationship between Sleep-Disordered Breathing and Lifestyle-related Illnesses in Subjects Who Have Undergone Health-screening
- Author
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Keisaku Fujimoto, Akemi Takamizawa, Mitsuyo Okada, Keishi Kubo, Kenji Tsushima, and Kazuhisa Urushihata
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Adult ,Male ,medicine.medical_specialty ,Polysomnography ,Hyperlipidemias ,medicine.disease_cause ,Body Mass Index ,Hypoxemia ,Sleep Apnea Syndromes ,Risk Factors ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Obesity ,cardiovascular diseases ,Life Style ,Aged ,Glucose Metabolism Disorders ,medicine.diagnostic_test ,business.industry ,Fatty liver ,Apnea ,General Medicine ,Middle Aged ,medicine.disease ,nervous system diseases ,respiratory tract diseases ,Apnea–hypopnea index ,Hypertension ,Cardiology ,Physical therapy ,medicine.symptom ,business ,Nasal cannula ,Hypopnea ,Body mass index - Abstract
Simplified sleep polysomnography was performed in 207 adult men to examine the relationship between the frequency of sleep-disordered breathing (SDB) and lifestyle-related illness.Each subject was checked for SDB using a simplified sleep polysomnograph (Auto-Set Portable; Teijin Limited, Tokyo, Japan). Apnea and hypopnea were detected with a nasal cannula type airflow sensor. Hypoxemia was checked with a percutaneous oxygen saturation (SpO2) monitor. We analyzed the relationships between SDB and body mass index (BMI) and hypertension, hyperlipidemia, liver dysfunction, fatty liver, and abnormal glucose metabolism.Fifty-nine subjects (29%) showed SDB with apnea hypopnea index (AHI) over 15 times/h. The frequency of obesity (BMIor = 25), hypertension, hypercholesterolemia, fasting blood glucose level, and HbA1c were significantly higher in patients with SDB than in normal individuals (AHI5 times/h). The frequencies of hypertension, hyperlipidemia, and abnormal glucose metabolism were compared between the obesity-free normal AHI group and the SDB group, and only that of hypertension was significantly different between the two groups.The present study revealed a high frequency of SDB among Japanese individuals. The results also suggest that as SDB becomes severe, it becomes more closely linked to the onset of lifestyle-related illnesses, such as hypertension, hypercholesterolemia and abnormal glucose metabolism.
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- 2006
4. Primary Malignant Lymphoma in the Posterior Mediastinum
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Keishi Kubo, Takayuki Honda, Akemi Takamizawa, Keisaku Fujimoto, Satoshi Kawakami, Tomonobu Koizumi, and Y. Maruyama
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Lymphoma ,Radiography ,Mediastinal tumor ,Scintigraphy ,Mediastinal Neoplasms ,Asymptomatic ,hemic and lymphatic diseases ,medicine ,Humans ,medicine.diagnostic_test ,business.industry ,Soft tissue ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Mediastinal Neoplasm ,respiratory tract diseases ,Female ,Radiology ,medicine.symptom ,Tomography, X-Ray Computed ,business - Abstract
We present a case of primary malignant lymphoma arising from the posterior mediastinum. A 63-year-old woman was admitted to our hospital for further examination of her chest radiographic findings. She was asymptomatic. Chest computed tomography (CT) and magnetic resonance imaging (MRI) showed a posterior mediastinal mass in the right thoracic paravertebral region. A percutaneous needle biopsy was performed under CT. The histological findings with immunochemical staining revealed a B cell type of diffuse malignant lymphoma. 67Ga scintigraphy revealed accumulation in this area, suggesting the posterior mediastinum as the origin of the disease. Reports of primary malignant lymphoma in the posterior mediastinum are extremely rare. The radiographic characteristics are discussed.
- Published
- 2004
5. METHOTREXATE STIMULATES LUNG EPITHELIAL CELLS TO RELEASE INFLAMMATORY CELL CHEMOTACTIC ACTIVITIES
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Sekiya Koyama, Takateru Izumi, Masayuki Haniuda, Akemi Takamizawa, Etsuro Sato, Sonoko Nagai, Makoto Kurai, Akihiro Tsukadaira, and Hiroki Numanami
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Pulmonary and Respiratory Medicine ,Antimetabolites, Antineoplastic ,medicine.medical_treatment ,Clinical Biochemistry ,Inflammation ,Granulocyte ,Biology ,Tumor Cells, Cultured ,medicine ,Humans ,Interleukin 8 ,Enzyme Inhibitors ,Antibodies, Blocking ,Molecular Biology ,A549 cell ,Leukotriene ,Dose-Response Relationship, Drug ,Monocyte ,Epithelial Cells ,respiratory system ,Eosinophil ,Pulmonary Alveoli ,Methotrexate ,medicine.anatomical_structure ,Cytokine ,Immunology ,Cancer research ,Chemokines ,medicine.symptom - Abstract
Methotrexate-induced pneumonitis has been reported as an infrequent but potentially serious complication of therapy in a variety of malignant and benign conditions. Because inflammatory cell infiltration is concerned with the development of methotrexate-induced pneumoinitis, and because airway epithelial cells participate in the orchestration of lung inflammation, the authors determined whether methotrexate might stimulate airway epithelial cells (A549 cells) to release neutrophil, monocyte, and eosinophil chemotactic activities (NCA, MCA, and ECA). A549 cells released NCA, MCA, and ECA in a dose- and time-dependent manner in response to methotrexate. Partial characterization revealed the heterogeneity of NCA, MCA, and ECA. The release of chemotactic activity was blocked by lipoxygenase inhibitors and cycloheximide. NCA was inhibited by leukotriene (LT) B(4) receptor antagonist, and anti-interleukin (IL)-8 and granulocyte colony-stimulating factor (G-CSF) antibodies. MCA was attenuated by LTB(4) receptor antagonist, and anti-monocyte chemoattractant protein (MCP)-1 and granulocyte-macrophage CSF (GM-CSF) antibodies. ECA was attenuated by LTB(4) receptor antagonist, and anti-IL-8 and GM-CSF antibodies. The release of IL-8, G-CSF, MCP-1, GM-CSF, and LTB(4) from A549 cells significantly increased in response to methotrexate. The mRNA expression of IL-8 and MCP-1 was augmented by methotrexate stimulation. These data suggest that type II epithelial cells may modulate inflammatory cell recruitment into the lung by releasing NCA, MCA, and ECA in response to methotrexate.
- Published
- 2003
6. Cyclophosphamide stimulates lung fibroblasts to release neutrophil and monocyte chemoattractants
- Author
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Sekiya Koyama, Takeshi Masubuchi, Sonoko Nagai, Akemi Takamizawa, Etsuro Sato, and Takateru Izumi
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Pulmonary and Respiratory Medicine ,Cyclophosphamide ,Neutrophils ,Physiology ,Pulmonary toxicity ,Enzyme-Linked Immunosorbent Assay ,Biology ,Antibodies ,Monocytes ,Cell Line ,chemistry.chemical_compound ,Alkylating antineoplastic agent ,Transforming Growth Factor beta ,Physiology (medical) ,Granulocyte Colony-Stimulating Factor ,medicine ,Humans ,Chemokine CCL5 ,Lung ,Chemokine CCL2 ,Chemotactic Factors ,Dose-Response Relationship, Drug ,Chemotaxis ,Monocyte ,Interleukin-8 ,Granulocyte-Macrophage Colony-Stimulating Factor ,Cell Biology ,Fibroblasts ,Nitrogen mustard ,Granulocyte colony-stimulating factor ,Granulocyte macrophage colony-stimulating factor ,medicine.anatomical_structure ,chemistry ,Immunology ,Toxicity ,Chromatography, Gel ,Cancer research ,medicine.drug - Abstract
Cyclophosphamide is an alkylating antineoplastic agent used in several conditions. However, little is known about the mechanism of its pulmonary toxicity. In the present study, we determined that human lung fibroblasts release activity for neutrophils and monocytes in response to cyclophosphamide in a dose- and time-dependent manner. Checkerboard analysis revealed that both neutrophil and monocyte activities were chemotactic. The release of chemotactic activity was inhibited by lipoxygenase inhibitors and cycloheximide. Molecular-sieve column chromatography revealed that both neutrophil (NCA) and monocyte (MCA) chemotactic activities had multiple peaks. NCA was inhibited by a leukotriene B4receptor antagonist and anti-interleukin-8 and anti-granulocyte colony-stimulating factor antibodies. MCA was attenuated by a leukotriene B4receptor antagonist and anti-monocyte chemoattractant protein-1 and anti-granulocyte-macrophage colony-stimulating factor antibodies. The concentrations of interleukin-8, granulocyte colony-stimulating factor, monocyte chemoattractant protein-1, and granulocyte-macrophage colony-stimulating factor significantly increased in response to cyclophosphamide. These data suggest that lung fibroblasts may modulate inflammatory cell recruitment into the lung by releasing NCA and MCA in response to cyclophosphamide.
- Published
- 2001
7. A rare case of muconodular lung adenocarcinoma
- Author
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Emiko Nakamura, Kyoichi Miyagawa, Toshio Shimizu, Kenji Kawaguchi, Akemi Takamizawa, and Jun Kanemoto
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Pathology ,medicine.medical_specialty ,Lung ,medicine.anatomical_structure ,business.industry ,Rare case ,medicine ,Adenocarcinoma ,medicine.disease ,business - Abstract
背景:日本肺癌学会では, 管腔内に粘液分泌が著明であるか, 癌細胞が粘液内に浮遊する傾向が著しい粘液産生性腺癌を粘液結節性腺癌として分類している. これはWHO分類にはなく, 報告例が少ないためその臨床病理学的特徴は不明瞭である.症例:70歳の女性. 集団検診にて左上肺野の異常影を指摘され, 当科受診. 胸部CTにて, 左肺S3cに内部に気管支透亮像を有する不整な腫瘤影を認めた. 経気管支的ブラシ擦過細胞診では, 疑陽性で確診に至らなかったため, 胸腔鏡下左上葉部分切除を予定した. しかし, 胸腔鏡検査時の特異的な肉眼所見より粘液産生性腺癌と診断. その場で左上葉切除術が施行され, 病理組織にて粘液結節性肺腺癌と診断された.結論:本腫瘍は, 細胞・組織学的には粘液産生の高度な細気管支肺胞上皮癌を主体とする孤立結節性高分化腺癌である. その胸腔鏡像や病理所見は特徴的であり, 予後良好の可能性がある肺腺癌の一型と考えられる.
- Published
- 2001
8. Cutaneous nocardiosis in the chest wall effectively diagnosed by needle aspirate. A case report
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Emiko Nakamura, Jun Kanemoto, Toshio Shimizu, Akemi Takamizawa, Kenji Kawaguchp, and Kyoichi Miyagawa
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medicine.medical_specialty ,Cutaneous nocardiosis ,business.industry ,Medicine ,business ,Surgery - Abstract
背景:穿刺吸引細胞診が有用であった, 本邦でもまれな胸部皮下ノカルジア症の1例について報告する.症例:症例は79歳男性, 検診にて尿蛋白を指摘, ネフローゼ症候群の疑いで当院を受診した.ステロイド剤による治療を開始したが, 開始後7ヵ月頃から, 右前腕の疹痛と側胸部皮下に6cm大の腫瘤が出現した.診断目的のため, 穿刺吸引細胞診を施行したところ好中球, リンパ球, マクロファージ, 変性壊死物質に混じって繊細な糸状の菌糸が認められた.菌糸はPAS染色, Grocott染色, Gram染色, Ziehl-Neelsen染色 (Fite法) 陽性を示しNocardiaが強く疑われた.細菌培養の結果からNocardia faroinioaと確認された.結論:ステロイド剤や免疫抑制剤, 抗がん剤治療を受けている患者に膿瘍中に繊細な菌糸が認められた場合, ノカルジア症である可能性も考慮すべきである.
- Published
- 2000
9. Markers Indicating Deterioration of Pulmonary Mycobacterium avium-intracellulare Infection
- Author
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Keishi Kubo, Hiroshi Yamamoto, Shusuke Sone, Takayuki Honda, Yoshitaka Yamazaki, and Akemi Takamizawa
- Subjects
Male ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Pathology ,CA-19-9 Antigen ,Lymphocyte ,Mycobacterium avium-intracellulare infection ,Blood Sedimentation ,Critical Care and Intensive Care Medicine ,Gastroenterology ,Body Mass Index ,Internal medicine ,medicine ,Humans ,Lung ,Tuberculosis, Pulmonary ,Aged ,Mycobacterium avium-intracellulare Infection ,Retrospective Studies ,Bronchiectasis ,medicine.diagnostic_test ,business.industry ,Respiratory disease ,Sputum ,Middle Aged ,medicine.disease ,C-Reactive Protein ,medicine.anatomical_structure ,Bronchoalveolar lavage ,Erythrocyte sedimentation rate ,Disease Progression ,Female ,medicine.symptom ,Tomography, X-Ray Computed ,business ,Bronchoalveolar Lavage Fluid - Abstract
To predict the natural history of pulmonary Mycobacterium avium-intracellulare (MAI) infection with nodular bronchiectasis, we retrospectively evaluated clinical manifestations, laboratory data, and bronchoalveolar lavage fluid (BALF) findings in 57 patients. The patients received follow-up chest computed tomographic scans and testing for sputum bacteriology between intervals of at least 12 mo. They were divided into two groups after observation for 28 +/- 13 mo: deteriorated (n = 34) and not-deteriorated (n = 23). There were no patients with spontaneous improvement. At the start of observation, the mean age was greater in the deteriorated group (69 +/- 9 yr) than in the not-deteriorated group (57 +/- 9 yr). The mean body-mass index was lower in the deteriorated group (19.2 +/- 3.1 kg/m(2)) than in the not-deteriorated group (21.5 +/- 1.5 kg/m(2)). C-reactive protein, erythrocyte sedimentation rate, and carbohydrate antigen 19-9 were significantly elevated in the deteriorated group. The BALF findings of the deteriorated group showed that the neutrophil cell counts were significantly increased. Thirty-four of 57 patients with pulmonary MAI infection with nodular bronchiectasis had progressive clinical and/or radiographic disease. The older and thinner patients tended to become worse. Neutrophil-related inflammation associated with a decrease in CD4+ lymphocyte might reflect the progression of pulmonary MAI infection with nodular bronchiectasis.
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- 1999
10. Human lung fibroblasts release chemokinetic activity for monocytes constitutively
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Tsuyoshi Masubuchi, Etsuro Sato, Sonoko Nagai, Takateru Izumi, Hiroshi Nomura, Sekiya Koyama, Keishi Kubo, and Akemi Takamizawa
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Adult ,Pulmonary and Respiratory Medicine ,Time Factors ,Physiology ,Leukotriene B4 ,T-Lymphocytes ,Receptors, Leukotriene B4 ,In Vitro Techniques ,Biology ,Antibodies ,Monocytes ,Cell Line ,chemistry.chemical_compound ,Transforming Growth Factor beta ,Physiology (medical) ,medicine ,Diethylcarbamazine ,Humans ,Masoprocol ,Trypsin ,Lipoxygenase Inhibitors ,Cycloheximide ,Platelet Activating Factor ,Fibroblast ,Chemokine CCL5 ,Lung ,Chemokine CCL2 ,Monocyte ,Granulocyte-Macrophage Colony-Stimulating Factor ,Chemotaxis ,Cell Biology ,Fibroblasts ,Molecular biology ,In vitro ,Chemotaxis, Leukocyte ,Kinetics ,medicine.anatomical_structure ,Granulocyte macrophage colony-stimulating factor ,Eicosanoid ,chemistry ,Immunology ,Liberation ,medicine.drug - Abstract
We determined whether human lung fibroblasts (HLFs) might release mediators that are responsible for monocyte chemokinetic activity (MCA) constitutively. HLF supernatant fluids showed MCA in a time-dependent manner ( P 4(LTB4)-receptor antagonist attenuated the total MCA and the lowest molecular weight peak of MCA. The concentrations of LTB4were 153.4 ± 12.4 (24 h) and 212 ± 16.6 (72 h) pg/ml. These findings suggest that HLFs may modulate the recruitment of monocytes into the lung by releasing MCP-1, GM-CSF, TGF-β, and LTB4constitutively.
- Published
- 1998
11. Second-line chemotherapy of platinum compound plus CPT-11 following ADOC chemotherapy in advanced thymic carcinoma: analysis of seven cases
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Shintaro, Kanda, Tomonobu, Koizumi, Yoshimichi, Komatsu, Sumiko, Yoshikawa, Mitsuyo, Okada, Orie, Hatayama, Masanori, Yasuo, Kenji, Tsushima, Kazuhisa, Urushihata, Keishi, Kubo, Mari, Sasabayashi, and Akemi, Takamizawa
- Subjects
Adult ,Male ,Thymus Neoplasms ,Middle Aged ,Irinotecan ,Carboplatin ,Survival Rate ,Doxorubicin ,Vincristine ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Camptothecin ,Female ,Cisplatin ,Cyclophosphamide ,Aged ,Retrospective Studies - Abstract
Optimal chemotherapeutic regimen in thymic carcinoma remains uncertain and the efficacy of second line chemotherapy has not been established either.We retrospectively evaluated the efficacy of an irinotecan plus cisplatin or carboplatin (IP) regimen as a salvage treatment for patients with unresectable thymic carcinoma that progressed after cisplatin, doxorubicin, vincristine and cyclophosphamide (ADOC) chemotherapy. Seven patients with histologically confirmed thymic carcinoma that was resistant to or who had relapsed after initial chemotherapy with ADOC were treated with IP. The treatment consisted of irinotecan (CPT-11, 60 mg/m2, days 1, 8 and 15) and cisplatin (80 mg/m2, day 1) or carboplatin (AUC 4) intravenously every 4 weeks, for at least 2 cycles.Two patients achieved partial responses. Although another two patients showed a significant reduction of the primary thoracic lesion, the appearance of a new lesion was found in one and a metastatic lesion was unchanged in the other. Neutropenia over grade 3 was observed in all patients but none of the patients developed serious infections. There were no severe non-hematological toxicities, including diarrhea.We conclude that salvage chemotherapy may be useful in certain patients with thymic carcinoma and irinotecan may be a novel and alternative agent for relapsed thymic carcinoma.
- Published
- 2007
12. [Investigation of a pulmonary tuberculosis outbreak]
- Author
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Akemi, Takamizawa, Mitsuyo, Okada, Toshiya, Amari, and Kubo, Keishi
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Aged, 80 and over ,Male ,Cross Infection ,Early Diagnosis ,Tuberculin Test ,Humans ,Female ,Antibiotic Prophylaxis ,Contact Tracing ,Middle Aged ,Tuberculosis, Pulmonary ,Aged ,Disease Outbreaks - Abstract
Diagnoses of infectious tuberculosis (TB) patients were followed by thorough contact investigation on the basis of our hospital's Infectious Disease Manual ever since an infection of an inpatient with extended hospital stay was confirmed by a positive acid-fast sputum smear in October 1998. In September 2000, a nurse was found to have pulmonary TB and another was given a diagnosis of right tuberculous pleuritis the following November. Contact investigations were expedited among all hospital staff, families of the infected nurses, and all suspected inpatients. Five were diagnosed as TB, 8 were given chemoprophylaxis and 8 others required observation. The result verified a TB outbreak within the hospital, and management of TB infection control was re-enforced subsequently. We concluded that immediate contact investigation promoted successful early diagnosis, and reacknowledged the significance of the health supervision of all staff, operations including the environment and equipment control of the institution, and frequent contact and integration with the administration of the public health center. This experience enabled a useful revision of the disease manual for the future.
- Published
- 2005
13. Chemotherapy for advanced thymic carcinoma: clinical response to cisplatin, doxorubicin, vincristine, and cyclophosphamide (ADOC chemotherapy)
- Author
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Keisaku Fujimoto, Yasuki Takabayashi, Hisanori Saegusa, Shinji Yamaguchi, Yoshiki Hirose, Satoshi Yamagishi, Keishi Kubo, Jirou Hirayama, Kenji Tsushima, Thomonobu Koizumi, Akemi Takamizawa, Hiroshi Yamamoto, Yoshitaka Yamazaki, and Akihiro Tsukadaira
- Subjects
Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Vincristine ,Cyclophosphamide ,medicine.medical_treatment ,chemistry.chemical_compound ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Carcinoma ,Medicine ,Humans ,Doxorubicin ,Thymic carcinoma ,Aged ,Neoplasm Staging ,Chemotherapy ,business.industry ,Remission Induction ,Thymus Neoplasms ,Middle Aged ,medicine.disease ,Nitrogen mustard ,Surgery ,Survival Rate ,Regimen ,chemistry ,Female ,Cisplatin ,business ,medicine.drug - Abstract
The role of systemic chemotherapy and optimal regimen in thymic carcinoma remains uncertain. We evaluated the clinical responsiveness of ADOC (cisplatin, doxorubicin, vincristine, and cyclophosphamide) chemotherapy for advanced thymic carcinoma that have distant metastatic or unresectable lesions. From 1996 to 2000, we treated eight cases of thymic carcinoma. According to the classification by Masaoka et al., the clinical stage in one case was IVa, whereas the others were IVb. Histologic subtypes were as follows: four cases were squamous cell carcinoma, two cases were undifferentiated, and two were small-cell carcinoma. All patients received 50 mg/m2 of cisplatin and 40 mg/m2 of doxorubicin intravenously on day 1, 0.6 mg/m2 of vincristine intravenously on day 3, and 700 mg/m2 of cyclophosphamide intravenously on day 4, ADOC regimen, respectively, at 3- to 4-week intervals. Six patients obtained a partial response after ADOC chemotherapy and the overall clinical response rate was 75%. There were no life-threatening side effects noted. Cisplatin plus VP-16 chemotherapy (PVP) was performed in three cases before the ADOC regimen, but PVP chemotherapy did not show beneficial effects in two patients. Median survival time was 19 months. ADOC chemotherapy appears to have significant activity against thymic carcinoma.
- Published
- 2002
14. Hypersensitivity pneumonitis induced by spores of Lyophyllum aggregatum
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Tomonobu Koizumi, Keishi Kubo, Toshiya Amari, Kenji Tsushima, Yoshitaka Yamazaki, Akemi Takamizawa, and Keisaku Fujimoto
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Pulmonary and Respiratory Medicine ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Biopsy ,Critical Care and Intensive Care Medicine ,Pulmonary function testing ,Diagnosis, Differential ,Japan ,Diffusing capacity ,medicine ,Humans ,Lung ,Aged ,medicine.diagnostic_test ,business.industry ,Respiratory disease ,respiratory system ,Middle Aged ,Spores, Fungal ,medicine.disease ,respiratory tract diseases ,medicine.anatomical_structure ,Erythrocyte sedimentation rate ,Air Pollution, Indoor ,Sputum ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Chest radiograph ,business ,Agaricales ,Lung Volume Measurements ,Tomography, X-Ray Computed ,Bronchoalveolar Lavage Fluid ,Hypersensitivity pneumonitis ,Alveolitis, Extrinsic Allergic - Abstract
Objectives: Lyophyllum aggregatum (LA) is called Shimeji in Japanese and is eaten commonly as a mushroom. Shimeji mushrooms are cultivated in an indoor environment all year round. This study aimed to clarify the clinical features of hypersensitivity pneumonitis (HP) induced by LA. Patients and setting: Ten patients showed mild respiratory symptoms including dry cough, sputum, and low-grade fever. We tried to characterize the clinical features and the findings using chest high-resolution CT (HRCT), pulmonary function tests (PFTs), and BAL fluid (BALF) tests in patients with HP induced by LA. HP was diagnosed from clinical features, HRCT findings, BALF findings, lung histology, and lymphocyte stimulation tests (LSTs) for LA. Results: Laboratory findings showed mean ( SD) elevated levels of C-reactive protein (0.78 1.3 mg/dL), erythrocyte sedimentation rate (48 23 mm/h), and -globulin (26.9 7.6%). PFTs revealed a slight decrease in the percentage diffusing capacity of the lung for carbon monoxide, possibly due to the presence of epithelial granulomas in the alveoli. Although 4 of 10 patients showed normal findings on the chest radiograph (CXR), chest HRCT findings of all patients showed centrilobular small nodules and diffuse ground-glass opacities. The BALF testing revealed an increase in total cell counts, showing predominantly activated T lymphocytes. The CD4/CD8 cell ratio was significantly decreased (0.5 0.3). The results of the LSTs were positive in seven of seven cases. Conclusions: Since patients with HP induced by LA typically have mild respiratory symptoms and sometimes normal CXR findings, their conditions might remain undiagnosed. However, the chest HRCT images showed the typical subacute phase of HP. (CHEST 2001; 120:1085–1093)
- Published
- 2001
15. Smoke extract stimulates lung fibroblasts to release neutrophil and monocyte chemotactic activities
- Author
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Sonoko Nagai, Takateru Izumi, Akemi Takamizawa, Sekiya Koyama, Richard A. Robbins, Etsuro Sato, Takeshi Masubuchi, and Keishi Kubo
- Subjects
Physiology ,Neutrophils ,Neutrophile ,Pulmonary Fibrosis ,Gene Expression ,Pyridinium Compounds ,Monocytes ,Receptors, G-Protein-Coupled ,Fibrosis ,Transforming Growth Factor beta ,Tetrahydroisoquinolines ,Granulocyte Colony-Stimulating Factor ,Cycloheximide ,Chemokine CCL5 ,Lung ,Cells, Cultured ,Chemokine CCL2 ,Protein Synthesis Inhibitors ,Chromatography ,Phenylpropionates ,Chemotaxis ,Smoking ,respiratory system ,Granulocyte macrophage colony-stimulating factor ,medicine.anatomical_structure ,medicine.symptom ,Immunosuppressive Agents ,medicine.drug ,Pulmonary and Respiratory Medicine ,Inflammation ,Receptors, Cell Surface ,Platelet Membrane Glycoproteins ,Biology ,Leukotriene B4 ,Physiology (medical) ,Tobacco ,medicine ,Humans ,Interleukin 8 ,Monocyte ,Interleukin-8 ,Granulocyte-Macrophage Colony-Stimulating Factor ,Cell Biology ,Fibroblasts ,medicine.disease ,Isoquinolines ,Plants, Toxic ,Immunology ,Platelet Aggregation Inhibitors - Abstract
Accumulation of monocytes and neutrophils and fibrous distortion of the airway are characteristics of airway disease secondary to smoking. The presence of inflammatory cells and fibrosis correlate, and, therefore, we postulated that lung fibroblasts might release chemotactic activity for neutrophils and monocytes in response to smoke extract. To test this hypothesis, human fetal lung (HFL1) fibroblasts were cultured, and the supernatant fluid was evaluated for neutrophil (NCA) and monocyte (MCA) chemotactic activities with a blind well chamber technique. HFL1 fibroblasts released chemotactic activity in response to smoke extract in a dose- and time-dependent manner ( P < 0.05). Checkerboard analysis showed that the activity was predominantly chemotactic. Partial characterization of the released chemotactic activity revealed that the activity was partly heat labile, trypsin sensitive, and ethyl acetate extractable. Lipoxygenase inhibitors and cycloheximide inhibited the release of both NCA and MCA. Molecular-sieve chromatography revealed that NCA and MCA were heterogeneous. NCA was inhibited by anti-human interleukin (IL)-8 and anti-granulocyte colony-stimulating factor antibodies and a leukotriene (LT) B4-receptor antagonist. Anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) and anti-monocyte chemoattractant protein (MCP)-1 antibodies and an LTB4-receptor antagonist inhibited MCA. Immunoreactive IL-8, granulocyte colony-stimulating factor, GM-CSF, and MCP-1 significantly increased in culture supernatant fluid in response to smoke extract. Finally, smoke extract augmented the expression of mRNAs of IL-8, GM-CSF, and MCP-1. These data demonstrate that lung fibroblasts release NCA and MCA in response to smoke extract and suggest that lung fibroblasts may modulate the inflammatory cell recruitment into the lung.
- Published
- 1999
16. Procaterol inhibits IL-1beta- and TNF-alpha-mediated epithelial cell eosinophil chemotactic activity
- Author
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Takeshi Masubuchi, Keishi Kubo, Sonoko Nagai, Sekiya Koyama, Akemi Takamizawa, T Isumi, and Etsuro Sato
- Subjects
Pulmonary and Respiratory Medicine ,Intracellular Fluid ,medicine.medical_specialty ,Time Factors ,Procaterol ,Phosphodiesterase Inhibitors ,medicine.medical_treatment ,Radioimmunoassay ,Bronchi ,Enzyme-Linked Immunosorbent Assay ,Pharmacology ,Cell Line ,chemistry.chemical_compound ,Eosinophil migration ,Theophylline ,Internal medicine ,medicine ,Cyclic AMP ,Humans ,Cyclic adenosine monophosphate ,Interleukin 8 ,RNA, Messenger ,Chemokine CCL5 ,Dose-Response Relationship, Drug ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Tumor Necrosis Factor-alpha ,Interleukin-8 ,Granulocyte-Macrophage Colony-Stimulating Factor ,Epithelial Cells ,Eosinophil ,Adrenergic beta-Agonists ,Recombinant Proteins ,Eosinophils ,Chemotaxis, Leukocyte ,Cytokine ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Eosinophil chemotaxis ,business ,medicine.drug ,Interleukin-1 - Abstract
Theophylline inhibits eosinophilic infiltration into the bronchial wall. It is unknown whether this is mediated by a cyclic adenosine monophosphate (c-AMP)-dependent reduction in eosinophil chemotactic activity (ECA) from bronchial epithelial cells (BEC). Therefore the effect of a beta2-agonist, procaterol and theophylline on the release of ECA from a BEC line, BEAS-2B was evaluated in response to interleukin (IL)-1beta and tumour necrosis factor-alpha (TNF-alpha). ECA was assessed using a blind-well chemotactic chamber, and the release and gene expression of cytokines were evaluated by means of enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction. IL-1beta and TNF-alpha stimulated the release of ECA from BEAS-2B cells in a dose- and time-dependent manner. Procaterol and theophylline directly inhibited eosinophil migration to IL-1beta and TNF-alpha-conditioned medium. The pretreatment of BEAS-2B cells with the same concentrations of procaterol inhibited the release of ECA in a dose-dependent fashion. Anti-IL-8, anti-regulated on activation, normal T-cell expressed and secreted (RANTES), and anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) inhibited ECA. Procaterol inhibited the release of RANTES, GM-CSF and IL-8 in a dose-dependent fashion. The effect of theophylline was less potent. Procaterol augmented cAMP levels in BEAS-2B cells in a time- and dose-dependent manner. The expression of IL-8, RANTES, and GM-CSF messenger ribonucleic acid was not inhibited by procaterol and theophylline. These data indicate that procaterol and theophylline may directly inhibit eosinophil migration and that procaterol may further inhibit the release of eosinophil chemotactic activity from BEAS-2B cells via a cyclic adenosine monophosphate-dependent mechanism. This warrants further studies on the involvement of bronchial epithelial cells in the anti-inflammatory effects of procaterol and theophylline in patients with asthma.
- Published
- 1999
17. Bleomycin stimulates lung epithelial cells to release neutrophil and monocyte chemotactic activities
- Author
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Sekiya Koyama, Etsuro Sato, Takeshi Masubuchi, Takateru Izumi, Keishi Kubo, Akemi Takamizawa, and Sonoko Nagai
- Subjects
Pulmonary and Respiratory Medicine ,Antimetabolites, Antineoplastic ,Physiology ,Antimetabolites ,Neutrophils ,medicine.medical_treatment ,Receptors, Leukotriene B4 ,Inflammation ,Lung injury ,Biology ,Bleomycin ,Leukotriene B4 ,Cell Line ,chemistry.chemical_compound ,Fibrosis ,Physiology (medical) ,medicine ,Humans ,Interleukin 8 ,Lung ,Chemotactic Factors ,Epidermal Growth Factor ,Monocyte ,Epithelial Cells ,Cell Biology ,respiratory system ,medicine.disease ,Monocyte Chemoattractant Proteins ,ErbB Receptors ,medicine.anatomical_structure ,Cytokine ,chemistry ,Immunology ,Cancer research ,Cytokines ,medicine.symptom - Abstract
Although bleomycin, an antineoplastic drug, is used in the treatment of a variety of tumors, the mechanisms of bleomycin-induced lung injury and fibrosis are not fully elucidated. We postulated that bleomycin might stimulate A549 cells, a type II pneumocyte cell line, to release neutrophil and monocyte chemotactic activities (NCA and MCA, respectively). To test this hypothesis, A549 cell supernatant fluids were harvested and evaluated for NCA and MCA. A549 cell supernatant fluids showed NCA and MCA in response to bleomycin in a dose- and time-dependent manner ( P < 0.05). Checkerboard analysis revealed that both NCA and MCA were predominantly chemotactic. Partial characterization of the released NCA and MCA showed that the activities were partially heat labile, trypsin digested, and predominantly ethyl acetate extractable. Lipoxygenase inhibitors and cycloheximide inhibited the release of chemotactic activities significantly. Molecular-sieve column chromatography revealed that the released activities were heterogeneous. However, low-molecular-weight activity was prominent. Leukotriene B4-receptor antagonist, anti-interleukin-8, anti-granulocyte colony-stimulating factor, and anti-monocyte chemoattractant protein-1 antibodies attenuated the chemotactic activities. Immunoreactive leukotriene B4receptor, interleukin-8, granulocyte colony-stimulating factor, and monocyte chemoattractant protein-1 significantly increased in supernatant fluids in response to bleomycin. These data demonstrate that bleomycin stimulates type II epithelial cells to release chemotactic activities and plays a role in inflammatory cell recruitment into the lung.
- Published
- 1999
18. Bleomycin stimulates lung fibroblasts to release neutrophil and monocyte chemotactic activity
- Author
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Akemi Takamizawa, Sekiya Koyama, Etsuro Sato, Takeshi Masubuchi, Keishi Kubo, Morie Sekiguchi, Sonoko Nagai, and Takateru Izumi
- Subjects
Neutrophils ,Immunology ,Interleukin-8 ,Granulocyte-Macrophage Colony-Stimulating Factor ,Fibroblasts ,Leukotriene B4 ,Monocytes ,Bleomycin ,Chemotaxis, Leukocyte ,Transforming Growth Factor beta ,Granulocyte Colony-Stimulating Factor ,Immunology and Allergy ,Humans ,Lung ,Chemokine CCL2 - Abstract
We determined whether human lung fibroblasts might release chemotactic activity for neutrophils (NCA) and monocytes (MCA) in response to bleomycin. The human lung fibroblasts supernatant fluids were evaluated for chemotactic activity by a blind well chamber technique. Human lung fibroblasts released NCA and MCA in a dose- and time-dependent manner in response to bleomycin. Checkerboard analysis of supernatant fluids revealed that both NCA and MCA were chemotactic. Partial characterization revealed that NCA was partly heat labile, trypsin sensitive, and predominantly ethyl acetate extractable. In contrast, MCA was partly trypsin sensitive and ethyl acetate extractable. The release of chemotactic activity was inhibited by lipoxygenase inhibitors and cycloheximide. Molecular sieve column chromatography revealed that both NCA and MCA had multiple chemotactic peaks. NCA was inhibited by leukotriene B4 receptor antagonist and anti-IL-8 and G-CSF Abs. MCA was attenuated by leukotriene B4 receptor antagonist, and monocyte chemoattractant protein-1, GM-CSF, and TGF-β Abs. Leukotriene B4 receptor antagonist and these Abs inhibited the corresponding m.w. chemotactic activity separated by column chromatography. The concentrations of IL-8, G-CSF, monocyte chemoattractant protein-1, GM-CSF, and TGF-β in the supernatant fluids significantly increased in response to bleomycin. These data suggest that lung fibroblasts may modulate inflammatory cell recruitment into the lung by releasing NCA and MCA in response to bleomycin.
- Published
- 1999
19. Smoke extract stimulates lung epithelial cells to release neutrophil and monocyte chemotactic activity
- Author
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Sekiya Koyama, Sonoko Nagai, Takateru Izumi, Akemi Takamizawa, Morie Sekiguchi, Etsuro Sato, Takeshi Masubuchi, and Keishi Kubo
- Subjects
Monocyte Chemoattractant Proteins ,Time Factors ,Leukotriene B4 ,Receptors, Leukotriene B4 ,Receptors, Cell Surface ,Platelet Membrane Glycoproteins ,Biology ,Granulocyte ,Antibodies ,Pathology and Forensic Medicine ,Receptors, G-Protein-Coupled ,chemistry.chemical_compound ,Smoke ,Tobacco ,medicine ,Humans ,Interleukin 8 ,Lipoxygenase Inhibitors ,Platelet Activating Factor ,Lung ,Cells, Cultured ,A549 cell ,Leukotriene ,Dose-Response Relationship, Drug ,Monocyte ,Type-II Pneumocytes ,Interleukin-8 ,Epithelial Cells ,respiratory system ,Molecular biology ,Plants, Toxic ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Chromatography, Gel ,Cytokines ,Regular Articles - Abstract
Inflammatory cells accumulate within the lungs of cigarette smokers. Current concepts suggest that these cells can induce protease-antiprotease and/or oxidant-antioxidant imbalance(s), which may damage the normal lung alveolar and interstitial structures. Because type II pneumocytes line the alveolar space, and because the inflammatory cells migrate and reside at the alveolus, we postulated that the type II pneumocytes might release chemotactic activity for neutrophils and monocytes in response to smoke extract. To test this hypothesis, A549 cells were cultured and the supernatant fluids were evaluated for the neutrophil and monocyte chemotactic activity (NCA and MCA) by a blind-well chamber technique. A549 cells released NCA and MCA in response to smoke extract in a dose- and time-dependent manner (P < 0.05). Checkerboard analysis showed that the activity was chemotactic. Partial characterization of NCA and MCA revealed that the activity was partly heat labile, trypsin sensitive, and ethyl acetate extractable. Lipoxygenase inhibitors and cycloheximide inhibited the release of NCA and MCA. Molecular sieve column chromatography showed multiple peaks for both NCA and MCA. NCA was inhibited by anti-human-interleukin (IL)-8 antibody, granulocyte colony-stimulating factor (G-CSF) antibody, or leukotriene (LT)B4 receptor antagonist. Monocyte chemoattractant protein (MCP)-1 antibody or LTB4 receptor antagonist inhibited MCA. Immunoreactive IL-8, G-CSF, MCP-1, and LTB4 significantly increased in the supernatant fluids in response to smoke extract. These data suggest that the type II pneumocytes may release NCA and MCA and modulate the inflammatory cell recruitment into the lung.
- Published
- 1998
20. Alveolar type II-like cells release G-CSF as neutrophil chemotactic activity
- Author
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Sekiya Koyama, Takeshi Masubuchi, Etsuro Sato, Keishi Kubo, Sonoko Nagai, Takateru Izumi, and Akemi Takamizawa
- Subjects
Pulmonary and Respiratory Medicine ,Lipopolysaccharides ,medicine.medical_specialty ,Physiology ,Leukotriene B4 ,Neutrophils ,Granulocyte ,Biology ,In Vitro Techniques ,Bradykinin ,Antibodies ,chemistry.chemical_compound ,Interferon-gamma ,Mice ,Physiology (medical) ,Internal medicine ,Granulocyte Colony-Stimulating Factor ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Cells, Cultured ,A549 cell ,Lung ,Phenylpropionates ,Tumor Necrosis Factor-alpha ,Interleukin-8 ,Interleukin ,Chemotaxis ,Epithelial Cells ,Cell Biology ,respiratory system ,Granulocyte colony-stimulating factor ,Pulmonary Alveoli ,Chemotaxis, Leukocyte ,Kinetics ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Cell culture - Abstract
We evaluated the potential of A549 cells, an alveolar type II epithelial cell line, to release granulocyte colony-stimulating factor (G-CSF), in addition to interleukin (IL)-8 and leukotriene B4, as neutrophil chemotactic activity (NCA). Human recombinant IL-1β stimulated A549 cells to release NCA in a time- and dose-dependent fashion. The released NCA was blocked by mouse anti-human G-CSF polyclonal antibody. Molecular-sieve column chromatography revealed that IL-1β induced the release of a 19- to 20-kDa chemotactic mass that was inhibited by anti-human G-CSF antibody. IL-1β stimulated the release of G-CSF in a dose-dependent fashion, but the time-dependent profile of G-CSF showed that the concentration of G-CSF declined after 48 h. Tumor necrosis factor (TNF)-α, Escherichia coli lipopolysaccharide (LPS), and bradykinin (BK) stimulated A549 cells to release NCA that was inhibited by anti-G-CSF antibody. The release of G-CSF in response to TNF-α, LPS, and BK was significantly increased. The similar concentrations of human recombinant G-CSF (10–1,000 pg/ml) as in the supernatant fluid induced neutrophil chemotaxis. G-CSF mRNA was expressed time and dose dependently at 4 h and declined after 4 h in response to IL-1β as evaluated by RT-PCR. The expression of G-CSF mRNA was also observed by TNF-α, LPS, and BK stimulation. These data suggest that type II alveolar epithelial cells may produce G-CSF as NCA and may participate in the regulation of leukocyte extravasation.
- Published
- 1998
21. Pulmonary infection with Mycobacterium avium-intracellulare leads to air trapping distal to the small airways
- Author
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Hiroshi Yamamoto, Takayuki Honda, Tomonobu Koizumi, Akemi Takamizawa, Takeshi Masubuchi, M. Hasegawa, Keishi Kubo, Shusuke Sone, Yoshitaka Yamazaki, Yukinori Matsuzawa, and Keisaku Fujimoto
- Subjects
Pulmonary and Respiratory Medicine ,Thorax ,Lung Diseases ,Pathology ,medicine.medical_specialty ,Vital capacity ,Bronchiole ,Functional Residual Capacity ,Nitrogen ,Vital Capacity ,Maximal Midexpiratory Flow Rate ,Peak Expiratory Flow Rate ,Critical Care and Intensive Care Medicine ,Air trapping ,Pulmonary function testing ,FEV1/FVC ratio ,Forced Expiratory Volume ,Medicine ,Humans ,Lung volumes ,Maximal Expiratory Flow Rate ,Mycobacterium avium-intracellulare Infection ,business.industry ,Respiratory disease ,Total Lung Capacity ,Bronchial Diseases ,Maximal Voluntary Ventilation ,respiratory system ,Middle Aged ,medicine.disease ,respiratory tract diseases ,Bronchiectasis ,Respiratory Function Tests ,Airway Obstruction ,Residual Volume ,medicine.anatomical_structure ,Inhalation ,Female ,medicine.symptom ,business ,Nuclear medicine ,Tomography, X-Ray Computed - Abstract
To clarify the structure and function of the airways in Mycobacterium avium-intracellulare (MAI) infection, we performed pulmonary function tests and high-resolution computed tomography (HRCT) of the thorax in female patients 61 +/- 9 yr of age (n = 12) with pulmonary MAI infection without predisposing lung disease and compared their data with those of normal female volunteers 54 +/- 8 yr of age (n = 9). We calculated the E/I ratio, i.e., the average ratio of HRCT number at full expiration to that at full inspiration, as an index for the evaluation of air trapping distal to the small airways. Patients showed significant increases in residual volume and slope of phase III (DeltaN2) of the single-breath nitrogen test, and significant decreases in flow at 50 and 25% of FVC, suggesting hyperinflation and obstruction of the small airways. HRCT of patients revealed the small nodules and ectasis of bronchioles and small bronchi located mainly in segments (S) S2, S3, S4, and S5. The E/I ratio was significantly elevated in patients, and especially higher in the upper lung field than in the lower lung field, suggesting air trapping distal to the small airways. The difference of E/I ratio between the upper and lower field is probably related to the segmental distribution of CT abnormalities. These findings suggest that MAI infection can lead to air trapping distal to the small airways.
- Published
- 1998
22. Catamenial Hemoptysis
- Author
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Tsutomu, Hachiya, Mitsuyo, Okada, Akemi, Takamizawa, Minoru, Hasegawa, Takayuki, Honda, and Keishi, Kubo
- Subjects
Adult ,Lung Diseases ,Hemoptysis ,Cysts ,Endometriosis ,Internal Medicine ,Humans ,Female ,General Medicine ,Tomography, X-Ray Computed ,Menstrual Cycle - Published
- 2003
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