1. Δ160p53 is a novel N-terminal p53 isoform encoded by Δ133p53 transcript.
- Author
-
Marcel V, Perrier S, Aoubala M, Ageorges S, Groves MJ, Diot A, Fernandes K, Tauro S, and Bourdon JC
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Dogs, Gene Knockdown Techniques, Humans, K562 Cells, Mice, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Isoforms chemistry, Protein Isoforms genetics, Protein Isoforms metabolism, RNA, Messenger genetics, Rats, Sequence Deletion, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 chemistry, Tumor Suppressor Protein p53 genetics
- Abstract
p53 gene expresses several protein isoforms modulating p53-mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, Δ160p53, lacking the first 159 residues. By knockdown experiments and site-directed mutagenesis, we show that Δ160p53 is encoded by Δ133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of Δ160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and Δ133p53 protein expression but not the one of Δ160p53. Overall, these results show that the Δ133p53 transcript generates two different p53 isoforms, Δ133p53 and Δ160p53., (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF