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Δ160p53 is a novel N-terminal p53 isoform encoded by Δ133p53 transcript.
- Source :
-
FEBS letters [FEBS Lett] 2010 Nov 05; Vol. 584 (21), pp. 4463-8. Date of Electronic Publication: 2010 Oct 13. - Publication Year :
- 2010
-
Abstract
- p53 gene expresses several protein isoforms modulating p53-mediated responses through regulation of gene expression. Here, we identify a novel p53 isoform, Δ160p53, lacking the first 159 residues. By knockdown experiments and site-directed mutagenesis, we show that Δ160p53 is encoded by Δ133p53 transcript using ATG160 as translational initiation site. This hypothesis is supported by endogenous expression of Δ160p53 in U2OS, T47D and K562 cells, the latter ones carrying a premature stop codon that impairs p53 and Δ133p53 protein expression but not the one of Δ160p53. Overall, these results show that the Δ133p53 transcript generates two different p53 isoforms, Δ133p53 and Δ160p53.<br /> (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Dogs
Gene Knockdown Techniques
Humans
K562 Cells
Mice
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Isoforms chemistry
Protein Isoforms genetics
Protein Isoforms metabolism
RNA, Messenger genetics
Rats
Sequence Deletion
Tumor Suppressor Protein p53 metabolism
Tumor Suppressor Protein p53 chemistry
Tumor Suppressor Protein p53 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 584
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 20937277
- Full Text :
- https://doi.org/10.1016/j.febslet.2010.10.005