66 results on '"Adriano do Nascimento Simões"'
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2. Characterization of growth and development of pumpkin cv Mini Jack fruits
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Genilza Almeida da Graça, Pryanka Thuyra Nascimento Fontes, Alysson Caetano Soares, Mônica Silva de Jesus, Patrícia Nogueira Matos, Maria Terezinha Santos Leite Neta, Adriano do Nascimento Simões, Luciana Marques de Carvalho, Luiz Fernando Ganassali de Oliveira Júnior, Marcelo Augusto Gutierrez Carnelossi, and Aline de Almeida Vasconcelos
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Curcubita moschata ,Respiratory rate ,Harvest point ,Post-harvest physiology ,Food processing and manufacture ,TP368-456 - Abstract
The physiological changes that occur during the growth and development of Mini Jack pumpkins fruits can lead to post-harvest losses, and shorten their shelf life. Thus, this work aimed to characterize the growth and development of the pumpkin cv Mini Jack. For this, fruits were harvested at 0, 5, 10, 15 and 20 days after anthesis (DAA) and evaluated for respiratory rate, height, diameter, weight, skin color, firmness, skin and pulp thickness, concavity fruit, pH, acidity, total soluble solids, carotene in the skin and pulp, reducing sugars, non-reducing sugars, and total sugars. During the growth and development of the fruits, there was an increase in the levels of total sugars, total soluble solids, and changes in carotene levels up to 10 DAA. The levels of total carotenoids increased significantly throughout the development of the fruits reaching the highest values at 20 DAA. It was possible to verify that the respiratory rate of the fruits was high at the beginning of development presenting reduction and stabilization at 10 DAA. The weight, height and concavity of the fruits increased up to 10 DAA. Thus, the ideal point for harvesting the fruits of the Mini Jack pumpkin occurred between 10 and 15 DAA.
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- 2024
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3. Primeiro registro de Aricoris campestris (Bates, 1868) (Lepidoptera: Riodinidae), e ocorrência de insetos danosos na cultura da pitaia (Hylocereus polyrhizus) no Sertão Pernambucano, Brasil
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Igor Tenório Marinho da Rocha, Adriano do Nascimento Simões, José Raliuson Inácio Silva, Maynar Nogueira Rodrigues de Carvalho, Flávio Pereira da Mota Silveira, Remy Farias de Souza, and Natanael Lucena Ferreira
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fruta-do-dragão ,Cactácea ,semiárido ,pragas agrícolas ,Agriculture ,Agriculture (General) ,S1-972 ,Veterinary medicine ,SF600-1100 - Abstract
A pitaieira (Hylocereus polyrhizus) é uma cactácea com grande destaque no mercado de frutas atual. Possui elevada rusticidade e tolerância às condições edafoclimáticas adversas possuindo potencial para o seu cultivo nas regiões semiáridas. Apesar de ser pouco acometida por pragas, a sua exploração comercial pode ser prejudicada se medidas de controle não forem realizadas. Ademais, produtos fitossanitários não foram registrados para o controle de pragas nesta cultura. Devido a isto, o conhecimento de insetos que acometem esta cultura é de fundamental importância para o seu correto controle e o sucesso produtivo do pomar. O objetivo deste estudo foi realizar um levantamento entomofaunístico visando avaliar a ocorrência de insetos danosos em um pomar de pitaia implantado no sertão Pernambucano. A avaliação ocorreu por meio de registros fotográficos, coleta e identificação dos insetos por meio de dados na literatura. Os registros e coletas foram realizadas unicamente no período diurno. Os insetos observados foram: lagarta Aricoris campestris (Bates, 1868) (Lepidoptera: Riodinidae), formiga Atta sexdens rubropilosa (Forel, 1908) (Hymenoptera: Formicidae), arapuá Trigona spinipes (Fabricius, 1793) (Hymenoptera: Apidae), pulgão Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae). Este trabalho relata o primeiro registro de lagartas de A. campestris, formiga, arapuá e pulgão causando danos na pitaieira.
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- 2024
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4. Physiological Responses of Minimally Processed Sweet Potatoes Grown with Increasing Doses of Phosphate Fertilizer
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Valecia Nogueira Santos-Silva, Pablo Henrique de Almeida Oliveira, Welder de Araújo Rangel Lopes, Aline Lima Soares, Natanael Lucena Ferreira, Ariel Sharon de Araújo Nogueira Marcelino, Sarah Alencar de Sá, Fred Augusto Lourêdo de Brito, Daniel A. Jacobo-Velázquez, Aurélio Paes Barros-Júnior, Lindomar Maria da Silveira, and Adriano do Nascimento Simões
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Chemistry ,QD1-999 - Published
- 2023
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5. Economic Aspects of Zinc Oxide Fertilization in Yam (Dioscorea alata L.) in a Semi-Arid Region of Brazil
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Antônio Lourenço Bezerra, João Everthon da Silva Ribeiro, Ester dos Santos Coêlho, Elania Freire da Silva, Pablo Henrique de Almeida Oliveira, Gisele Lopes dos Santos, Antonio Gideilson Correia da Silva, José Travassos dos Santos Júnior, Ivanice da Silva Santos, Felipe Alves Reis, Lindomar Maria da Silveira, Aurélio Paes Barros Júnior, and Adriano do Nascimento Simões
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economic viability ,tuber production ,yield ,production costs ,foliar fertilization ,Plant culture ,SB1-1110 - Abstract
The management and improvement of yam productivity are associated with a good supply of essential nutrients for the growth and development of the crop that has economic viability. This research aimed to evaluate the economic feasibility of foliar fertilization with Zintrac® in two yam agricultural seasons (2022/2023 and 2023/2024). Therefore, two experiments were conducted at the Rafael Fernandes Experimental Farm, Mossoró, RN, Brazil. The experimental design was in a Latin square design with five treatments of doses of Zintrac® (0, 1, 2, 3, and 4 L ha−1) and five replications. Among the production costs of yams, labor and seed acquisition were the most significant. The highest profitability index was achieved with the dose of 1L Zintrac® ha−1 in the first season and second season, which corresponded to 78.97 and 57.86%. For the first season, increments were observed in all treatments that received zinc doses with increases of 48.70, 31.22, 14.30, and 15.93% for 1, 2, 3, and 4 L of Zintrac® ha−1 compared to the dose of 0 L ha−1. On the other hand, in the second season, there was an increase only in the dose of 1 L ha−1 of Zintrac®, which corresponded to 51.3% in the net yield (ha−1) of the dose of 0 L ha−1. Therefore, foliar zinc oxide fertilization was economically viable for the yam crop, obtaining higher economic indices at the dose of 1 L ha−1. The highest cost for growing yams is using a dose of 4 L ha−1 of Zintrac®, totaling USD 6977.59 (first season) and USD 6868.33 (second season)
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- 2024
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6. Application of melatonin and sucrose in prolonging the vase life of amaryllis cut flowers (Hippeastrum Hybridum Herb)
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Fred Augusto Lourêdo de Brito, Nilo Ricardo Corrêa de Mello Júnior, Lady Daiane Costa de Sousa Martins, Mayara Bernardo Tavares Martim, Luzia Ferreira da Silva, Thamilla Menezes Guerra, and Adriano do Nascimento Simões
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flower longevity ,phenolic compounds ,phytoregulator ,vase lif ,Plant culture ,SB1-1110 - Abstract
The effects of melatonin application by itself and mixed with sucrose solution on vase life and physicochemical changes in amaryllis cut flowers were investigated. Amaryllis stems with flower buds at harvest points were placed into vases containing different maintenance solutions: Melatonin (Mt; 0.01 mM), Sucrose (Suc; 4%), Melatonin + Sucrose (Mt + Suc; 0.01 mM + 4%) and distilled water (Control). Each treatment consisted of five repetitions, with one inflorescence stem per vase. The vases remained at 22 ± 3 °C and constant lighting of 13 µmol m-2 s-1 for 14 days. Immersion of stems in Suc or Mt + Suc delayed the initial senescence processes of the amaryllis cut flower. Vase life was prolonged for additional 2 days compared to stems immersed in water (control), increasing from 10 to 12 days of conservation. Floral stem mass was kept more stable; the degradation of anthocyanin and flavonoid pigments was delayed; and the stability of phenolic compounds and total carbohydrates was maintained for 14 days. These findings confirm the role of melatonin as a preservative in cut flowers. However, its preservative effect was potentialized by the addition of sucrose to the water of the amaryllis cut flowers. More work needs to be carried out to investigate the physiological mechanisms promoted by the interaction of melatonin and sucrose in the regulation of senescence in amaryllis cut flowers, including the analysis of gene expression and activity of the antioxidant system.
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- 2023
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7. Influence of irrigation, cladode size, harvest time and addition of citric acid on the properties of cactus mucilage
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Franquielle Ribeiro de Oliveira, Kelem Silva Fonseca, Alexandre Maniçoba da Rosa Ferraz Jardim, Jheizon Feitoza do Nascimento Souza, Ellen Karine Diniz Viégas, Anderson Rodrigo da Silva, and Adriano do Nascimento Simões
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nopalea cochenillifera (l.) salm-dyck ,cactaceae ,hydrocolloid ,titratable acidity ,Agriculture (General) ,S1-972 - Abstract
Cactus is a highly resistant crop, and offers benefits in its mucilage for the industry due to its physicochemical properties. This study aimed to analyze the effect of irrigation managements, harvest time, cladode size and citric acid addition during the processing on the agro-industrial yield and physicochemical properties of mucilage extracted from cladodes of Nopalea cochenillifera Miúda clone cultivated under rainfed and irrigated conditions. The yield, soluble solids content, titratable acidity, pH, K+ and Na+ contents, and the electrical conductivity were measured in the mucilage. The management practices in the field resulted in changes in the studied phytochemicals. For the rainfed cultivation, the night harvest of the biggest cladodes increased the K+and soluble solids contents. The addition of citric acid to the mucilage extraction resulted in higher amounts of soluble solids, titratable acidity and electrical conductivity and lower pH values. The highest water availability promoted higher mucilage yields.
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- 2022
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8. Growth and vase life of gladiolus plants cultivated under different conditions in the semi-arid region of Brazil
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Lady Daiane Costa de Sousa, Kelem Silva Fonseca, Luzia Ferreira da Silva, Thieres George Freire da Silva, Lucas Vinícius Pierre de Andrada, Agda Raiany Mota dos Santos, Patrícia Lopes Moreira Feitosa Apolinário, Jheizon Feitoza do Nascimento Souza, and Adriano do Nascimento Simões
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gladiolus x grandiflorus l. ,postharvest ,stem height ,relative water content ,dry mass ,Plant culture ,SB1-1110 - Abstract
Cultivation conditions are determining factors that affect the quality of cut flowers. The objective of this study was to evaluate the vegetative growth, flowering and vase life of commercial gladiolus cultivars under different shading conditions in the Brazilian semi-arid region. Two cultivars of gladiolus (Jester and Rose Friendship) were grown under two conditions: full sun and 70% shade. Biometrics were evaluated 60 days after planting and the growth rate of the crop was evaluated at 15-30, 30-45 and 45-60 days for the variables: number of tillers, stem diameter, number of leaves and plant height. After 45 days, the fresh weight, dry weight and total relative water content of the area and root parts of the plants were evaluated. After the harvest, the stems were kept at a temperature of 22±3 °C and relative humidity (RH) of 60%. Visual assessment, pH of the solution, variation in the volume of water and loss of fresh mass were measured every two days. Cultivation with 70% shading resulted in greater accumulation of biomass in the aerial part of gladiolus plants, greater neck diameter and height. The Rose Friendship cultivar underwent a greater translocation of dry matter to the aerial part of the plants, resulting in greater height. On the other hand, the gladiolus stems from cultivation under full sun conditions resulted in longer vase life, 10 days under conservation conditions at 22±3 °C and RH 60%.
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- 2021
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9. Inoculation of plant-growth-promoting rhizobacteria in Myracrodruon urundeuva Allemão supports in tolerance to drought stress
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Douglas Moreira de Oliveira, André Luiz Alves de Lima, Nathália Bandeira Diniz, Carolina Etienne de Rosália e Silva Santos, Sérgio Luiz Ferreira da Silva, and Adriano do Nascimento Simões
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Aroeira do Sertão tree ,Azospirillum lipoferum ,semi-arid ,oxidative stress ,membrane damage ,PGPR ,Plant culture ,SB1-1110 ,Plant ecology ,QK900-989 - Abstract
This study evaluated the influence of Azospirillum lipoferum on the growth of Myracroduon urundeuva (Anacardiaceae) plants under drought stress, by means of biometric, physical–chemical and biochemical parameters. The association of A. lipoferum with the roots of the plants provided increases of 30% root length, 50% root dry weight, 34% shoot dry weight and 10% soluble protein content. The inoculated plants still maintained 5% higher leaf water potential than those not inoculated and lower membrane damage. Furthermore, the inoculated plants shown less leaf fall and dark green leaves, confirmed by maintenance of the highest levels of chlorophyl a, b and total. On the other hand, superoxide dismutase activity was significantly lower in the inoculated plants, possibly due to the induction of a non-enzymatic protective feature. In this way, the inoculation of PGPR in M. urundeuva can be an alternative for the production of plants that are more tolerant to drought stress.
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- 2018
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10. Etanol e ácido cítrico aumentam a longevidade de gérberas cv. Mistique
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Rúbia Kécia Marins Maia, Edivan Amaro da Silva, Rafael Mateus Alves, Maria Aparecida dos Santos Morais, Marcos Ribeiro da Silva Vieira, Luzia Ferreira da Silva, Raquel Ferraz Jardim Simões, Kelem Silva Fonseca, and Adriano do Nascimento Simões
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Gerbera jamesonii ,flores ,conservação pós-colheita ,pulsing e manutenção. ,Plant culture ,SB1-1110 - Abstract
Etanol e ácido cítrico podem aumentar a longevidade em algumas flores de corte. Porém, seu uso deve ser de extremo cuidado, com métodos de aplicação e concentrações específicas para cada tipo de flor de corte. Assim, o objetivo foi estudar as mudanças físico-químicas e fisiológicas na longevidade de gérberas de corte cv. Mistique, submetidas a etanol (Et) e/ou ácido cítrico (AC). As hastes foram selecionadas e padronizadas a 35 cm de comprimento, mantidas a 20 ± 2 ºC e UR 65 ± 2%, sob iluminação contínua. As gérberas foram submetidas a dois métodos de aplicação (pulsing 48 horas e manutenção), sob diferentes concentrações de Et (4%, 6% e 8%) e/ou AC (100 e 200 mg L-1) e água destilada (controle), a cada dois dias foi realizada análise visual, longevidade, tombamento das hastes, massa fresca, conteúdo relativo de água, extravasamento de eletrólitos e atividades das enzimas peroxidase e polifenoloxidase. Observou-se que a solução de Et (4%) + AC (100 mg L-1), proporcionou maior longevidade, independente do método de aplicação. Estes resultados foram base para um terceiro experimento, no qual as hastes foram imersas por pulsing, em soluções de Et (4%) e/ou AC (100 mg L-1), água destilada (controle). Foi verificado que as gérberas submetidas à solução com Et + AC, apresentaram menor perda de massa fresca, maior conteúdo relativo de água, menor extravasamento de eletrólitos e aumento mais lento nas atividades da polifenoloxidase e peroxidase. Isto proporcionou retardo no tombamento das hastes e melhor aparência, resultando em maior longevidade, quando comparada as demais soluções.
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- 2019
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11. Effect of multilayer nylon packages on the oxidative damage of minimally processed yam
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Adriano do Nascimento Simões, Daniel Gomes Coelho, Kelem Silva Fonseca, Aurélio Paes Barros Júnior, Sergio Luiz Ferreira-Silva, Clarissa Soares Freire, and Edson Fabio da Silva
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Oxidative stress ,Enzymatic browning ,Catalase ,H2O2 ,Dioscorea spp ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Abstract Appropriate storage packaging is an important aspect to minimize physiological deterioration and enhance the shelf-life of minimally processed products. Thus, the goal of this study was to evaluate the physiological and biochemical changes associated with the quality loss of minimally processed yam, maintained in two different packages at 5 ± 2 °C. The yam roots were selected, washed and conserved at 8 ± 2 °C for 24 hours. They were then peeled and cut into slices about 3 cm thick and minimally processed. Approximately 300 g of minimally processed yam roots were packed in polypropylene (PP, 4 μm thick) and multilayer nylon (NY, 15 μm thick) 15 cm wide x 20 cm long bags, which were stored at 5 ± 2 °C for 14 days. The minimally processed yam conserved in PP packaging presented fluorescence on the surface of the segments, characteristic symptoms of Pseudomonas spp., and showed higher peroxidase and catalase activities. The nylon packaging was more efficient in reducing oxidative damage and also inhibited polyphenol oxidase activity and decreased the accumulation of soluble proteins, resulting in decreased deterioration during storage. Thus, the quality of the minimally processed yam maintained in NY packaging was conserved for 14 days at 5 ± 2 °C.
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- 2019
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12. APPLICATION OF ANTIOXIDANTS AND EDIBLE STARCH COATING TO REDUCE BROWNING OF MINIMALLY - PROCESSED CASSAVA
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DANIEL GOMES COELHO, MOAB TORRES DE ANDRADE, DOMINGOS FERREIRA DE MÉLO NETO, LUIZ FERREIRA-SILVA, and ADRIANO DO NASCIMENTO SIMÕES
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Agriculture ,Biology (General) ,QH301-705.5 - Abstract
This study aimed to evaluate the quality of minimally - processed cassava treated with antioxidants and a starch - based edible coating. Cassava roots were washed, cooled, immersed in cold water, peeled and then cut. Root pieces were then immersed in a chloride solution, centrifuged, and subsequently immersed in either a starch suspension (3%), a solution containing antioxidants (3% citric acid and 3% ascorbic acid), or in both the coating and antioxidant solutions. Coated root pieces were dried at 18 ± 2°C for 1 hour, then packaged into polypropylene bags (150 g per pack) and kept at 5 ± 2°C for 15 days, and assessed every 3 days. A completely randomized design was used in a 4 × 6 factorial consisting of the treatment (control, coating, antioxidant, or coating and antioxidant) and the storage period (0, 3 6, 9, 12 or 15 days), with three replicates in each group. The pH, blackened area and peroxidase and polyphenol oxidase activities of the cassava was reduced in treatments containing antioxidants and the scores of visual analysis and phenolic content were higher. Therefore, treatment with antioxidants was effective for reducing browning in minimally - processed cassava, retaining the quality of cassava pieces stored for 15 days at 5 ± 2°C. The combination of antioxidants and the edible coating showed no improvement compared to treatment with antioxidants alone.
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- 2017
13. QUALITY OF MINIMALLY PROCESSED YAM (Dioscorea sp.) STORED AT TWO DIFFERENT TEMPERATURES
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ADRIANO DO NASCIMENTO SIMÕES, CLARISSA SOARES FREIRE, EDSON FÁBIO DA SILVA, AURÉLIO PAES BARROS JÚNIOR, and SÉRGIO LUIZ FERREIRA-SILVA
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Agriculture ,Biology (General) ,QH301-705.5 - Abstract
This work studied the physical, chemical and bio chemical alterations in minimally processed yam stored at two different temperatures, as well a s the incidence of bacteria of the genus Pseudomonas . The experimental design was completely randomised in a 2x8 factorial design, with two storage temperature s (5 and 10°C) and eight storage times (0, 2, 4, 6, 8, 1 0, 12 and 14 days). Experiments were in triplicate. Yam was selected, peeled and cut into slices of approximate ly 3 cm thickness. The slices were rinsed with wate r, sanitised and then drained in kitchen strainers. Ap proximately 300 g of the processed product were pac ked in nylon multilayers 15 μ m thick, 15 cm wide and 20 cm long. The packs were sealed, weighed and kept at 5 and 10 ± 2°C for 14 days. Fresh weight loss, baking tim e, enzymatic activity of polyphenol oxidases, perox idases and catalases, total soluble phenol content, and an tioxidant capacity were evaluated, as well as visua l analysis and incidence of Pseudomonas sp. Means of temperatures were compared by Tukey ́s test at 5% significance. Yam storage at 5°C reduced weight loss and kept vis ual quality for longer; it also reduced cooking tim e and the activity of the enzymes polyphenol oxidase and pero xidase. In contrast, it promoted higher content of total soluble phenols, as well as a higher catalase activ ity and antioxidant capacity. During the storage ti me, there was no incidence of Pseudomonas sp. Minimally processed yam stored at 10°C may be sold for up to six days, and yam stored at 5ºC for up to 14 days.
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- 2016
14. Endogenous levels of polyamines during cold storage of bird-ofparadise treated with biocides
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Marcos Ribeiro da Silva Vieira, Giuseppina Pace Pereira Lima, Luiza Maria de Souza Fernandes, Angela Vacaro de Souza, Reginaldo Conceição Cerqueira, Pahlevi Augusto de Souza, Armando Reis Tavares, and Adriano do Nascimento Simões
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Strelitzia reginae ,inflorescence ,ornamental plant ,postharvest ,senescence. ,Plant culture ,SB1-1110 - Abstract
This study aimed to analyse the endogenous levels of free polyamines putrescine (Put), spermidine (spd) and spermine (spm) under the effect of 8-HQC (8-hydroxyquinoline citrate) and chlorine during cold storage of bird-of-paradise floral scapes. Flowers were selected, labelled and randomly distributed in recipients for postharvest trials. The base of flower scapes was immersed with pulsing treatments (Control, 100 chlorine, 250 or 500 mg L-1 8-HQC) for 48 h, thereafter, stored at 10.5 °C and 90% relative humidity for a period of 12 days. Flower parts were sampled for polyamines analysis at day 0, 4, 8 and 12 days during storage. All samples had higher Put levels than the control. Treatment with 500 mg L-1 8-HQC showed the highest Spd levels in bracts, while chlorine treatment had the highest Spm levels in stems.
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- 2017
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15. The effects of storage temperature on the quality and phenolic metabolism of whole and minimally processed kale leaves
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Adriano do Nascimento Simões, Silvino Intra Moreira, Paulo Roberto Mosquim, Nilda de Fátima Ferreira Soares, and Rolf Puschmann
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Brassica oleracea cv. acephala ,enzymatic browning ,peroxidase ,polyphenol oxidase ,phenylalanine ammonia-lyase ,Agriculture (General) ,S1-972 - Abstract
We studied the effects of storage temperature on the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in minimally processed kale (Brassica oleracea var. acephala) that was stored for 15 and 9 days at 5 ± 1ºC and 10 ± 1ºC, respectively. The main visual evidence for quality loss in whole leaves was yellowing and loss of turgescence. Minimally processed leaves presented significant browning, indicating increased POD and PPO activities. The PAL activity in minimally processed leaves stored at 5ºC was fourfold higher than that of whole leaves after two days of storage. We showed that minimal processing influenced PAL, POD and PPO activities. The activity of all enzymes studied increased during storage, indicating that changes in phenolic metabolism play an important role in the decline of kale quality. PAL activity increased rapidly at the beginning of storage and exhibited a reduced rate of increase over time, while the PPO and POD activities increased continuously over time. The storage at 5 ° C was a great ally in delaying changes in phenolic metabolism; however, the absolute PAL activity was higher at 5 than at 10ºC.
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- 2014
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16. Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage
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Mateus da Silva Junqueira, Adriano do Nascimento Simões, Tocio Sediyama, Paulo Cesar Côrrea, and Rolf Puschmann
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Manihot esculenta Crantz ,carotenoides ,PAL ,qualidade ,capacidade antioxidante ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
The aim of this study was to evaluate the effects of fresh-cut in stick form of two cassava cultivars on their biochemical and antioxidant characteristics, at two harvest times. The fresh cut cassava sticks were packaged in polypropylene, maintained at 5±1ºC, with 90±5% relative humidity for 12 days. The concentration of carotenoids, total soluble phenolic compounds and the activity of phenylalanine ammonia lyase and antioxidant capacity were significantly higher for the sticks of cultivar 'Amarela' cassava than for the sticks of cultivar 'Cacau'. The concentrations of carotenoids, total soluble phenolic compounds, and the activity of phenylalanine ammonia lyase and antioxidant capacity were all significantly greater when harvested at 14-month of age. There was a significant increase in the activity of phenylalanine ammonia lyase during the 12 days of storage at 5ºC. However, there was a decrease in total carotenoids, soluble phenolic compounds, and in the antioxidant capacity of the two cultivars.
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- 2014
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17. FRUIT JUICES AS AN ALTERNATIVE TECHNIQUE FOR CONSERVATION OF FRESH-CUT BANANA
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ANDERSON ADRIANO MARTINS MELO, LEONARDO THOMAZ DINIZ, ADRIANO DO NASCIMENTO SIMÕES, and ROLF PUSCHMANN
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Agriculture ,Biology (General) ,QH301-705.5 - Abstract
Browning discoloration after cutting is detrimental for the quality of a number of fruits and vegetables, such as banana, apple, pear, potato, and some roots such as cassava, yam, and others. Browning and softening compromise banana after cut shelf-life in a few hours under cold storage. Therefore, anti-browning compounds have been applied to slices before packing. Some commonly used substances are calcium chloride, ascorbic acid, cysteine and citric acid, in immersed inchemical mixtures. Recent studies have demonstrated the possibility of preserving fresh-cut banana immersed in sweetened fruit juice for relatively longer periods, favoring commercialization. This type of conservation, although widely used in Brazil for fruit salads, consists of a more complex system in a physiological basis, requiring adjustment of the solution parameters, such as sugar concentration, pH and acidity, considering the viability and freshness of the plant tissue. In this short review, we discuss some experimental data and present a new method for preserving fresh-cut banana. Reduction of enzymatic activity, either in temporary dipping treatment or permanent immersion of banana slices is regarded as a key factor for maintaining its quality during cold storage.
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- 2014
18. QUALIDADE DE RAÍZES DE MANDIOCA DE MESA MINIMAMENTE PROCESSADA NOS FORMATOS MINITOLETE E RUBIENE
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CLARISSA SOARES FREIRE, ADRIANO DO NASCIMENTO SIMÕES, MARCOS RIBEIRO DA SILVA VIEIRA, AURÉLIO PAES BARROS JÚNIOR, and FRANCISCLEUDO BEZERRA DA COSTA
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Agriculture ,Biology (General) ,QH301-705.5 - Abstract
This study aimed to quantify the agro - industrial income and evaluating the quality of cassava minimally processed in minitolete and rubiene shapes. The assays were performed at the Academic Unit of Serra Talhada/ Federal Rural University of Pernambuco. Roots of sweet cassava cv. Mossoró were harvested at 14 months of age, minimally processed in minitolete and rubiene shapes and stored at 5 ± 2 °C and 90 ± 5 % of relative humidity for 11 days. Agroindustrial income, accumulated fresh weight, pH, soluble solids, soluble sugars and cooking time were evaluated. The experimental design was completely randomized in 2x6 factorial design, referring to shapes (minitolete and rubiene) and refrigerated storage times (0, 3, 5, 7, 9 and 11 days), respectively, with 3 replications. The agroindustrial income for each shape was calculated only at the end of minimal processing. The cooking time was measured at 0 and 11 days in 2x2 factorial design. The formats 'minitolete' and 'rubiene' minimally processed sweet cassava showed agroindustrial income above 60%. There- fore, it is suggested the minimum processing of sweet cassava in those shapes. Furthermore, the quality of the shapes was kept preserved throughout the 11 days of refrigerated storage. The 'rubiene' favored higher mean values of soluble sugars giving this format greater sweetness.
- Published
- 2014
19. Influência da temperatura de armazenamento na qualidade das inflorescências de estrelítzia (Strelitzia reginae Banks ex Aiton)
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Marcos Ribeiro da Silva Vieira, Giuseppina Pace Pereira Lima, Angela Vacaro de Souza, Nelson Geraldo de Oliveira, and Adriano do Nascimento Simões
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strelitzia reginae ,flores ,conservação ,senescência ,Agriculture ,Biology (General) ,QH301-705.5 - Abstract
O presente trabalho tem por objetivo de estudar a influência da temperatura de armazenamento (câmara fria e temperatura ambiente) na qualidade das inflorescências de estrelítzia. Escapos florais foram selecionados, etiquetados e descartados quanto à presença de danos mecânicos, doenças e/ou pragas. Transcorrido esse período, foram transferidos para recipientes, onde foram submetidos a dois experimentos. No experimento 1, escapos foram colocados em baldes contendo água da rede pública e levadas para câmara fria a 7,5 ºC e UR de 90%, por um período de doze dias. No experimento 2, os escapos foram mantidos nas mesmas condições, porém em temperatura ambiente por um período de seis dias. Nos dois experimentos, as análises visuais: coloração, brilho, manchas (através da atribuição de notas), abertura e queda de floretes (contagem) foram avaliadas em intervalo de quatro dias em câmara fria e a cada 48 horas em condições de temperatura ambiente. A sépala foi o órgão que apresentou maior perda na coloração. A variável brilho apresentou o mesmo comportamento em relação aos dois experimentos. Maiores incidências de manchas nas inflorescências ocorreram em temperatura ambiente. Os escapos apresentaram aumento no número de floretes abertos em câmara fria. Essa tendência não ocorreu em temperatura ambiente. Não foram observados diferenças na queda de floretes. Conclui-se que a temperatura de armazenamento não contribui para a qualidade pós-colheita de estrelítzia.
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- 2014
20. TEMPO DE COLHEITA COMO MODULADOR DE FITOQUÍMICOS EM CULTIVARES DE BATATA DOCE PARA A INDÚSTRIA
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VALÉCIA NOGUEIRA SANTOS, KELEM SILVA FONSECA, SARAH ALENCAR DE SÁ, JHEIZON FEITOZA DO NASCIMENTO SOUZA, AURÉLIO PAES BARROS JÚNIOR, and ADRIANO DO NASCIMENTO SIMÕES
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Ipomoea batatas (L.) Lam. Browning. Carotenoids. Phenolic compounds ,Compostos fenólicos ,Ipomoea batatas (L.) Lam ,Ipomoea batatas (L.) Lam. Escurecimento. Carotenóides. Compostos fenólicos ,Browning ,Escurecimento ,General Agricultural and Biological Sciences ,Carotenoids ,Carotenóides ,Phenolic compounds - Abstract
As a fresh food, sweet potatoes are highlighted for their taste and appearance. In the industry, they are valued for their starch and sugar content. In both scenarios, much attention has been given to the composition of bioactive compounds. The application of abiotic stresses seems to induce an overproduction of these compounds in some vegetables. The objective was to examine the influence of harvest time on the synthesis and accumulation of bioactive compounds in varieties of minimally processed sweet potatoes with different flesh colors. Sweet potato cultivars (Beterraba, Jerimum, Mãe de Família Roxa, and BRS Cuia) were harvested at 120, 150, and 180 days after planting, then subjected to minimal processing and stored at 5 ºC for 10 days. The most recommended time for harvesting sweet potatoes in semi-arid conditions for minimal processing by the industry is between 150 and 180 days. This is the period when the sweet potatoes exhibit the best quality for fresh consumption, as detected in ‘Mãe de Família Roxa’ and ‘Jerimum’. ‘BRS Cuia’ harvested at 120 days showed the highest phenolic compound levels, the highest polyphenol oxidase and peroxidase enzyme activities, and the most intense browning symptoms. In addition to its starch content, it can be used as a raw material for the extraction of phenolic compounds of interest. ‘Beterraba’ showed low sugar values, rendering them unfavorable for fresh consumption or minimal processing. However, this cultivar also exhibited a high content of carotenoids, making them bio-factories for the food and pharmaceutical industries. RESUMO Como alimento fresco, a batata-doce se destaca pelo sabor e aparência. Na indústria, é valorizado por seu teor de amido e açúcar. Em ambos os cenários, muita atenção tem sido dada à composição dos compostos bioativos. A aplicação de estresses abióticos parece induzir uma superprodução desses compostos em algumas hortaliças. O objetivo foi examinar a influência da época de colheita na síntese e acúmulo de compostos bioativos em variedades de batata-doce minimamente processadas com diferentes cores de polpa. As cultivares de batata-doce: Beterraba, Jerimum, Mãe de Família Roxa e BRS Cuia foram colhidas aos 120, 150 e 180 dias após o plantio, submetidas ao processamento mínimo e armazenadas a 5 ºC por 10 dias. A época mais recomendada para a colheita da batata-doce em condições semi-áridas para a indústria de processamento mínimo foi entre 150 e 180 dias, período em que a batata-doce apresentou melhor qualidade para consumo in natura em destaque para 'Mãe de Família Roxa' e 'Jerimum'. A ‘BRS Cuia’ colhida aos 120 dias apresentou os maiores teores de compostos fenólicos, as maiores atividades das enzimas polifenoloxidase e peroxidase e os sintomas de escurecimento mais intensos, podendo ser utilizado como matéria-prima para a extração de compostos fenólicos de interesse, além do amido. A ‘Beterraba’ apresentou baixos valores de açúcar, tornandoos desfavoráveis para consumo in natura ou processamento mínimo. No entanto, esta cultivar também apresentou um alto teor de carotenóides, tornando-os biofábricas para as indústrias alimentícia e farmacêutica.
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- 2022
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21. Preparation and characterization of a biodegradable film from cactus Nopalea sp
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Fred Augusto Lourêdo de Brito, Yara Panta de Araújo, Ariel Sharon de Araújo Nogueira Marcelino, Natanael Lucena Ferreira, Kelem Silva Fonseca, Andréa Monteiro Santana Silva Brito, Thieres George Freire da Silva, and Adriano do Nascimento Simões
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The increasing use of natural biodegradable polymers is a consequence of the concern of society with sustainability. Mucilage of forage cactus of the genus Nopalea is an attractive polymer matrix for the composition of edible films. Most studies on biodegradable films are carried out with the genus Opuntia. This makes Nopalea an important source of interest in the investigation of film formation. The objective was to study the formulation of films and evaluation of physical, optical, and mechanical characteristics of clones of Nopalea and Opuntia, through the addition of water and glycerol. The mucilage was extracted from cladodes of Nopalea cochenillifera Salm Dyck: clones IPA Sertânia (IPA) and Miúda (MIU); and, from Opuntia stricta [Haw.] Haw., clone Orelha de Elefante Mexicana (OEM). The powdered mucilage was hydrated and vacuum filtered, followed by the formulation of the films, without glycerol (just water) and with the addition of glycerol at 15%, 25%, and 14% for IPA, MIU, and OEM clones, respectively. The yield of powdered mucilage was higher for Nopalea. Optical tests with the films revealed that the addition of glycerol generated slightly yellow films (positive b* values), with less transparency than the control films, regardless of the clone and higher L* value in IPA clone films. Moisture content and thickness were increased when glycerol was added. All films, with and without glycerol, showed high water solubility. The microstructure revealed that the films with glycerol presented more compact, smooth, and linear surfaces, forming a homogeneous network. The FTIR spectra revealed that the glycerol films showed the same profile as the control samples, but with higher absorption intensities in some bands. The results found in the present work evidence the potential of the genus Nopalea to produce biodegradable films, as already widely known for the genus Opuntia.
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- 2022
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22. Characterization of mucilage from clones of Opuntia and Nopalea prickly pear cactus harvested in different seasons in Brazilian semiarid
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Yara Panta de Araújo, Andréa Monteiro Santana Silva Brito, Kelem Silva Fonseca, Jheizon Feitoza do Nascimento Souza, Lady Daiane Costa de Sousa, Thieres George Freire da Silva, Danielle Da Silva Eugênio, and Adriano Do Nascimento Simões
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This study proposes to characterize hydrated and refrigerated mucilage obtained from cladodes of clones of prickly pear cactus harvested during the drizzle and dry seasons in the semiarid of Brazil. Cladodes of Opuntia stricta [Haw.] Haw (Orelha de Elefante Mexicana [OEM] clone) and Nopalea cochenillifera Salm Dyck (IPA Sertânia [IPA] and Miúda [MIU] clones) were harvested at 6 am and extracted mucilage. The main bands in the infrared region were characterized. Physicochemical analyses were performed on day zero and at 12 days. Cladodes harvested in the dry season showed higher mucilage yield and soluble solid, total soluble carbohydrate, and K+, for the three clones. The OEM clone also exhibited significant increases in pH, Na+, and electrical conductivity when harvested in the dry season than in the wet season. In addition, the mucilage extracted from the Opuntia cladodes did not have changes in the carbohydrate, titratable acidity, or total soluble protein levels. The IPA and MIU clones, in turn, were characterized by parameters that remained stable during conservation (phenolic compounds, titratable acidity, K+ and Na+). The spectroscopic profile was similar for all studied clones. The principal component analysis allowed the formation of clusters between seasons and conservation times. It is suggested that the cladodes of the genus Nopalea showed better potential in the manufacture of edible films and coatings. The cladodes of the genus Opuntia, for the use of mucilage as ingredients in foods such as bread, pasta, and others, making them with better functional properties. Therefore, these factors should be considered for the use of mucilage in the industry.
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- 2021
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23. Use of the abrasion technique in minimal processing as an alternative to increase purchase acceptability and minimize browning in yam
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Sérgio Luiz Ferreira-Silva, Lucas Vinicius Pierre de Andrada, Rogério de Aquino Saraiva, André Luiz Alves de Lima, Maria Aparecida dos Santos Morais, Kelem Silva Fonseca, Rosilene Alves de Medeiros, and Adriano do Nascimento Simões
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Food Handling ,030309 nutrition & dietetics ,Abrasion (mechanical) ,Polyphenol oxidase activity ,Oxidative damage ,03 medical and health sciences ,Enzymatic antioxidant ,0404 agricultural biotechnology ,Phenols ,Browning ,Humans ,Food science ,0303 health sciences ,Control treatment ,Nutrition and Dietetics ,Dioscorea ,Chemistry ,04 agricultural and veterinary sciences ,CELL DEBRIS ,Consumer Behavior ,040401 food science ,Plant Tubers ,Agronomy and Crop Science ,Catechol Oxidase ,Food Science ,Biotechnology - Abstract
BACKGROUND The present study investigated the sensory acceptance, oxidative damage and protection, and possible anatomical-structural damage of cells from the surface of shapes of minimally processed yam. The tubers were minimally processed into the peeled rondelle, dice and 'chateau cut' (chateau) shapes, the latter of which was obtained after performing the abrasion technique. Control treatment corresponded to the rondelle shape with the periderm. The pieces were kept packed at 5 ± 2 °C for 14 days. RESULTS Peeled rondelle and chateau were sensorially the most well-accepted yam shapes and achieved the highest purchase intention. The enzymes were partially modulated by the detected H2 O2 levels. Oxidative burst lasted longer in the minimally processed tissues than in the control. Polyphenol oxidase activity showed a clear difference in behavior between the minimally processed pieces and the control. Minimal processing induced transient increases in phenolic compounds, for which the expression was lowest in the abraded pieces. On the other hand, these pieces exhibited greater cell collapse on the surface of the amyliferous parenchyma. CONCLUSION Based on the results of the trained panel, the abrasion technique is an alternative to provide shapes that are better accepted and marketable, more resistant to browning, and can be stored for up to 12 days. Resistance to browning may be related to a more efficient modulation of enzymatic antioxidant systems and intense deposition of cell debris on the surface of the amyliferous parenchyma. © 2021 Society of Chemical Industry.
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- 2021
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24. Growth and vase life of gladiolus plants cultivated under different conditions in the semi-arid region of Brazil
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Adriano do Nascimento Simões, Lucas Vinicius Pierre de Andrada, Jheizon Feitoza do Nascimento Souza, Thieres George Freire da Silva, Agda Raiany Mota dos Santos, Luzia Ferreira da Silva, Patrícia Lopes Moreira Feitosa Apolinário, Lady Daiane Costa de Sousa, and Kelem Silva Fonseca
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0106 biological sciences ,Gladiolus x grandiflorus L ,teor relativo de água ,Vegetative reproduction ,Plant Science ,Cut flowers ,Horticulture ,Biology ,massa seca ,01 natural sciences ,postharvest ,SB1-1110 ,dry mass ,Dry weight ,altura de hastes ,Cultivar ,Gladiolus ,pós-colheita ,gladiolus x grandiflorus l ,relative water content ,Vase life ,fungi ,Plant culture ,food and beverages ,Sowing ,04 agricultural and veterinary sciences ,biology.organism_classification ,stem height ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Shading ,010606 plant biology & botany - Abstract
Cultivation conditions are determining factors that affect the quality of cut flowers. The objective of this study was to evaluate the vegetative growth, flowering and vase life of commercial gladiolus cultivars under different shading conditions in the Brazilian semi-arid region. Two cultivars of gladiolus (Jester and Rose Friendship) were grown under two conditions: full sun and 70% shade. Biometrics were evaluated 60 days after planting and the growth rate of the crop was evaluated at 15-30, 30-45 and 45-60 days for the variables: number of tillers, stem diameter, number of leaves and plant height. After 45 days, the fresh weight, dry weight and total relative water content of the area and root parts of the plants were evaluated. After the harvest, the stems were kept at a temperature of 22±3 °C and relative humidity (RH) of 60%. Visual assessment, pH of the solution, variation in the volume of water and loss of fresh mass were measured every two days. Cultivation with 70% shading resulted in greater accumulation of biomass in the aerial part of gladiolus plants, greater neck diameter and height. The Rose Friendship cultivar underwent a greater translocation of dry matter to the aerial part of the plants, resulting in greater height. On the other hand, the gladiolus stems from cultivation under full sun conditions resulted in longer vase life, 10 days under conservation conditions at 22±3 °C and RH 60%. Resumo As condições de cultivo são fatores determinantes que afetam a qualidade das flores de corte. Diante disso, objetivou-se, neste estudo, avaliar o crescimento vegetativo, floração e vida de vaso de variedades de gladíolos comerciais no semiárido brasileiro, sob diferentes condições de sombreamento. Duas variedades de gladíolos (Jester e Rose Friendship) foram cultivadas em duas condições: sol pleno e 70% de sombreamento. Avaliou-se a biometria em 60 dias após o plantio e a taxa de crescimento da cultura aos 15-30, 30-45 e 45-60 dias para as variáveis: número de perfilhos, diâmetro do colo, número de folhas e altura da planta. Após 45 dias, avaliou-se a massa fresca, massa seca e conteúdo relativo de água total da parte área e da parte radicular das plantas. Após a colheita, as hastes foram mantidas em temperatura de 22±3 °C e umidade relativa (UR) de 60%. A cada dois dias, realizou-se avaliação visual, além de avaliação do pH da solução, variação no volume de água e perda de massa fresca. O cultivo com 70% de sombreamento resultou em maior acúmulo de biomassa na parte aérea das plantas de gladíolos, maior diâmetro do colo e altura. A variedade Rose Friendship resultou em maior translocação de massa seca para a parte aérea das plantas. Isso resultou em maior altura. Por outro lado, as hastes de gladíolos provenientes do cultivo nas condições de pleno sol resultaram em maior vida de vaso: 10 dias em condições de conservação a 22±3 °C e UR 60%.
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- 2021
25. Phosphate Fertilization as a Modulator of Enzymatic Browning in Minimally Processed Cassava
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Sérgio Luiz Ferreira-Silva, Kelem Silva Fonseca, Danielle da Silva Eugênio, Aurélio Paes Barros-Júnior, Hugo Rafael Bentzen Santos, Valécia Nogueira Santos E Silva, Welder de Araújo Rangel Lopes, Adriano do Nascimento Simões, Flávio Pereira da Mota Silveira, and Ariel Sharon de Araújo Nogueira Marcelino
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Manihot ,biology ,Superoxide Dismutase ,Phosphorus ,chemistry.chemical_element ,General Chemistry ,Phosphate ,Catalase ,Polyphenol oxidase ,Phosphates ,Superoxide dismutase ,chemistry.chemical_compound ,chemistry ,Fertilization ,Browning ,biology.protein ,Food science ,General Agricultural and Biological Sciences ,Hydrogen peroxide ,Peroxidase - Abstract
This study proposes to relate the increase in phosphorus (P) supply in the soil, via phosphate fertilization, to oxidative damage and protection, phenylpropanoid metabolism, and enzymatic browning in minimally processed cassava. The roots were grown with 0, 60, and 120 kg ha-1 P2O5. The roots were harvested, and the yield and P content in the root, stem, and leaves were quantified. The roots were minimally processed and stored for 12 days at 5 °C. The higher supply of P in the soil increased the P content of roots and stems but not the agro-industrial yields. Roots grown at 120 kg ha-1 P2O5 showed higher detection of hydrogen peroxide, which was accompanied by increased phenolic compounds, soluble quinones, and antioxidant capacity and increased activities of the enzymes superoxide dismutase, catalase, ascorbate peroxidase, polyphenol oxidase, and peroxidase. The present study thus demonstrates the role of phosphorus application, induction of the synthesis of phenolic compounds, and quality of fresh-cut cassava.
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- 2021
26. Physicochemical, physiological and anatomical properties of three segments of peach palm for industrial use and minimal processing
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Aline Ellen Duarte de Sousa, Marília Contin Ventrella, Kelem Silva Fonseca, Adriano do Nascimento Simões, Milton E. Pereira-Flores, Alexandre Maniçoba da Rosa Ferraz Jardim, Anderson Adriano Martins Melo, and Rolf Puschmann
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Color difference ,Starch ,General Chemical Engineering ,010401 analytical chemistry ,Titratable acid ,04 agricultural and veterinary sciences ,Ascorbic acid ,Vascular bundle ,040401 food science ,01 natural sciences ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,Horticulture ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Respiration ,Safety, Risk, Reliability and Quality ,Respiration rate ,Palm ,Food Science - Abstract
Peach palm has regions adjacent to the heart-of-palm that are normally neglected by the industry, and have potential to be utilized in minimally processed form. We characterized three parts of peach palm, evaluating anatomical, physicochemical and physiological parameters aiming to increase the industrial yield and evaluate shelf-life of the fresh-cut product. Harvested rods were sectioned into apical, median and basal segments, and analyzed for yield, firmness, soluble solids, titratable acidity, vitamin C, sugars and starch. Segments were sampled for anatomy and measurement of ethylene and respiration rates. After fresh-cut operations, segments were stored for 15 days at 5 °C and evaluated for surface color and carbohydrate content. The total yield based on length and fresh-mass was 85% and 70%, respectively. Hearts of palm were firmer toward the edges of apical and basal regions; the median region was the softest (14 N). The median region showed the highest SSC, 8.6 oBrix and vitamin C, 9.4 mg ascorbic acid 100 g−1 FW, and maintained the highest ethylene production, either whole or sliced. Respiration rate of all regions decreased 1 h after segmentation, the apical region showed the highest rate, 364 mL CO2 kg−1 h−1, followed by median and basal. Yellowing and pronounced color difference occurred after two weeks of storage in all the studied regions. Partially differentiated vascular bundles were present in all regions. Based on firmness and chemical parameters, all regions have potential for use as minimally processed product and can be stored up to 7 days, maintaining the original color.
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- 2019
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27. ENSAIOS ENZIMÁTICOS DA POLIFENOLOXIDASE E PEROXIDASE EM CULTIVARES DE BATATA DOCE COLHIDAS EM DIFERENTES ÉPOCAS
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José Ricardo Tavares de Albuquerque, Adriano do Nascimento Simões, Samara Lopes de Almeida, Aurélio Paes Barros Júnior, Maria Aparecida dos Santos Morais, and Kelem Silva Fonseca
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Padronização ,Ipomoea batatas L. (Lam.). Standardization. Minimum processing ,Polyphenol oxidase ,Ipomoea batatas L ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Processamento mínimo ,Cultivar ,Hydrogen peroxide ,(Lam.) ,Catechol ,Chromatography ,biology ,Minimum processing ,food and beverages ,04 agricultural and veterinary sciences ,040401 food science ,Standardization ,Enzyme assay ,Ipomoea batatas L. (Lam.). Padronização. Processamento mínimo ,Point of delivery ,chemistry ,040103 agronomy & agriculture ,biology.protein ,0401 agriculture, forestry, and fisheries ,Guaiacol ,General Agricultural and Biological Sciences ,Peroxidase - Abstract
Enzyme assays are based on methodologies described in the literature. However, the enzyme kinetics must be adjusted to obtain more reliable results. This study aimed to adjust assays by testing different polyphenol oxidase (PPO) and peroxidase (POD) extract amounts and reaction times in sweet potato cultivars harvested at different times. Sweet potato cultivars Paraná, Mãe de Família, and ESAM1 were harvested at 120, 150, and 180 days after planting and minimally processed. A 0.25 g sample was used to determine PPO and POD activities immediately after minimal processing at each harvest. Extraction was performed in 1500 μL phosphate buffer (0.2 M, pH 6.0). The PPO assay was performed by adding 10-50 μL extract, 1490-1450 μL phosphate buffer (0.2 M, pH 6.0), and 1500 μL catechol (0.2 M). The POD assay was carried out by adding 10-50 μL extract to a reaction medium containing 1790-1750 μL phosphate buffer (0.2 M, pH 6), 100 μl guaiacol (5 g L-1), and 100 μL hydrogen peroxide (0.8 g L-1). In both cases, the evaluated reaction times were 1, 2, and 3 min. In the three cultivars, PPO and POD activities increased with the volume of extract and reaction time at all harvest times. The enzyme extract volume of 10 μL for 2 min promoted a continuous increase in PPO and POD enzyme activities in all studied cultivars and at all reaction times. RESUMO Ensaios enzimáticos são baseados em metodologias descritas na literatura. Entretanto, a cinética enzimática deve ser ajustada para resultados mais confiáveis. Objetivou-se ajustar os ensaios testando diferentes volumes de extrato de polifenoloxidase (PPO) e peroxidase (POD) e tempos de reação em cultivares de batata-doce colhidas em diferentes épocas. As cultivares Paraná, Mãe de Família e ESAM1 foram colhidas aos 120, 150 e 180 dias e minimamente processadas. Amostra de 0,25 g foi usada para determinar as atividades de PPO e POD imediatamente após o processamento mínimo em cada colheita. A extração foi realizada em 1500 μL de tampão fosfato (0,2 M, pH 6,0). O ensaio para PPO foi realizado pela adição de 10 a 50 μL de extrato, 1490 a 1450 μL de tampão fosfato (0,2 M, pH 6,0) e 1500 μL de catecol (0,2 M). O ensaio para a POD foi realizado adicionando 10-50 μL de extrato a um meio de reação contendo 1790 a 1750 μL de tampão fosfato (0,2 M, pH 6), 100 μl de guaiacol (5 g L-1) e 100 μL de peróxido de hidrogênio (0,8 g L-1). Em ambos os casos, os tempos de reação avaliados foram 1, 2 e 3 min. Nas três cultivares, as atividades da PPO e POD aumentaram com o volume de extrato e tempo de reação em todas as épocas de colheita. Alíquota de 10 μL de extrato por 2 minutos promoveu um incremento contínuo na atividade da PPO e POD em todas as cultivares e tempos de reação estudados.
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- 2019
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28. Mucilage of spineless cactus in the composition of an edible coating for minimally processed yam (Dioscorea spp.)
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Rúbia Kécia Marins Maia, Kelem Silva Fonseca, Samara Lopes de Almeida, Maria Aparecida dos Santos Morais, Ellen Karine Diniz Viégas, Adriano do Nascimento Simões, and Valécia Nogueira Santos E Silva
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biology ,Chemistry ,Starch ,General Chemical Engineering ,010401 analytical chemistry ,04 agricultural and veterinary sciences ,medicine.disease ,biology.organism_classification ,040401 food science ,01 natural sciences ,Polyphenol oxidase ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Mucilage ,Cactus ,medicine ,Cladodes ,Composition (visual arts) ,Dioscorea ,Dehydration ,Food science ,Safety, Risk, Reliability and Quality ,Food Science - Abstract
The objective of this study was to examine formulations of edible coatings based on spineless-cactus mucilage for the preservation of minimally processed yam. Cladodes of spineless cactus clone IPA-Sertânia were harvested, washed, sanitized, and subjected to mucilage extraction to be used as an edible coating. Yam roots were minimally processed and immersed into the following coating suspensions: cactus mucilage + cassava starch (3%) + glycerol (1%) or cactus mucilage solely. Control corresponded to immersion in water. Samples were dried, packed and stored at 5 ± 2 °C for 10 days. The biocoating containing cactus mucilage reduced dehydration and maintained the visual and sensory quality of the yam slices. Additionally, this hydrocolloid increased the amount of phenolic compounds and led to different responses between polyphenol oxidase and peroxidase. Therefore, the studied formulations containing cactus mucilage show to be promising for the composition of biofilms and application to minimally processed yam roots.
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- 2019
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29. Delaying the harvest induces bioactive compounds and maintains the quality of sweet potatoes
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Kelem Silva Fonseca, Igor Otavio Minatel, Giuseppina Pace Pereira Lima, José Ricardo Tavares de Albuquerque, Camila Renata Corrêa, Samara Lopes de Almeida, Cristine Vanz Borges, Maria Aparecida dos Santos Morais, Aurélio Paes Barros Júnior, Marla Silvia Diamante, Adriano do Nascimento Simões, Univ Fed Rural Pernambuco, Universidade Estadual Paulista (Unesp), and Fed Rural Univ Semiarid
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030309 nutrition & dietetics ,Biophysics ,Orange (colour) ,phenolic compounds ,Biology ,Shelf life ,Polyphenol oxidase ,minimal processing ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Browning ,Cultivar ,Ipomoea batatas ,white blush ,Solanum tuberosum ,Pharmacology ,browning ,0303 health sciences ,carotenoids ,food and beverages ,04 agricultural and veterinary sciences ,Cell Biology ,beta Carotene ,Carotenoids ,040401 food science ,Bioactive compound ,Horticulture ,Phytochemical ,chemistry ,Postharvest ,Food Science - Abstract
Made available in DSpace on 2020-12-10T20:02:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-17 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco Universidade Federal Rural de Pernambuco We have identified the best harvesting periods, related to higher bioactive compound content and post-harvest quality, for minimally processed sweet potatoes with orange pulp and cream pulp. Quality loss in minimally processed sweet potatoes occurred due to browning in cv. Mae de Familia and ESAM1 and to whitening in cv. Parana. Both Mae de Familia and ESAM1 presented higher peroxidase and polyphenol oxidase activities at 120 days, and cv. Parana contained high levels of provitamin A. beta-carotene was the major carotenoid (up to 99%) in orange pulp. All cultivars harvested at 150 and 180 days and minimally processed showed the best visual quality, the highest bioactive compound content, and no microbial infection. Delaying the harvest also increase the antioxidant activity in orange roots, which was associated with a high content of carotenoids and phenolic compounds, resulting in an increase in the nutritional quality of the product. Practical applications Postharvest techniques can improve the visual and phytochemical quality of vegetables and extend their shelf life. The present research provides the best harvesting periods for minimally processed cream and orange sweet potato roots with higher postharvest qualities. Delaying the harvest resulted in a product with better visual quality, higher levels of bioactive compounds, which are highly beneficial to health, for example, ss-carotene, and no microbiological contamination. Our results may contribute to improvements in the current knowledge about harvesting times for sweet potato growers who aim to produce high quality, minimally processed products with a longer shelf life. Univ Fed Rural Pernambuco, Dept Plant Prod, Serra Talhada, Brazil Sao Paulo State Univ, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil Fed Rural Univ Semiarid, Dept Plant Sci, Mossoro, Brazil Sao Paulo State Univ, Med Sch, Dept Pathol, Botucatu, SP, Brazil Sao Paulo State Univ, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil Sao Paulo State Univ, Med Sch, Dept Pathol, Botucatu, SP, Brazil CNPq: 305177/2015-0 CNPq: 307571/20190 CNPq: 423100/2018-1 FAPESP: 2016/22665-2 CAPES: 88881-159183/2017-01 Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco: APQ-0795-5.01/16 Universidade Federal Rural de Pernambuco: 807292073-15/PRPPG 015/2018
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- 2020
30. Physiological Effect of Kinetin on the Photosynthetic Apparatus and Antioxidant Enzymes Activities During Production of Anthurium
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Sérgio Luiz Ferreira-Silva, Miguel Alves Júnior, Eduardo Soares de Souza, Carlos A.V. de Souza, Marcos Ribeiro da Silva Vieira, Alexandre Tavares da Rocha, Adriano do Nascimento Simões, Luzia Ferreira da Silva, and Fabiana B. de Moura
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0106 biological sciences ,0301 basic medicine ,Stomatal conductance ,Antioxidant ,medicine.medical_treatment ,Plant Science ,lcsh:Plant culture ,Photosynthesis ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,lcsh:SB1-1110 ,Ecology, Evolution, Behavior and Systematics ,Transpiration ,Anthurium ,Ecology ,biology ,Renewable Energy, Sustainability and the Environment ,fungi ,food and beverages ,biology.organism_classification ,Enzyme assay ,Horticulture ,030104 developmental biology ,chemistry ,Cytokinin ,biology.protein ,Kinetin ,010606 plant biology & botany - Abstract
The results observed in the literature raise the hypothesis according to which cytokinin plays important roles in photosynthetic metabolisms and antioxidant enzymes. Thus, the study aimed to evaluate the effect of foliar application of the isolated cytokinin kinetin at the production cycle, seeking to analyze its effects on enzyme activity and photosynthetic parameters. The plants treated with CK presented reduction of leaf CO2 assimilation rate (Pn) and stomatal conductance (Gs), while that transpiration rate (Tr) was unaffected. The internal CO2 concentrations decreased with the increase in cytokinin levels, but were maintained under CK 50 mg·L−1. The plants treated with CK 75 mg·L−1 was verified higher carboxylation efficiency (Pn/Ci), which was associated to values of CO2 assimilation and transpiration unaltered. Apparent electron transport rate showed variations in the concentration of 25 mg·L−1. Considering the study of enzyme activity, on the other hand, it cannot be stated that kinetin has an effective action in delaying oxidative damage. It presents mixed results, since an efficiency in the application of cytokinin was not observed, presenting induction levels of ascorbate peroxidase activity. Thus, further research is needed to determine more precisely the effects of kinetin on gas exchange and antioxidant enzymes in anthurium plants. Keywords: anthurium, kinetin, photosynthesis, senescence, plant hormone, oxidative stress
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- 2018
31. Quality of sweet potato cultivars planted harvested at different times of two seasons
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Giordanio Bruno Silva Oliveira, Elizangela Cabral dos Santos, Patrícia Ligia Dantas de Morais, José Ricardo Tavares de Albuquerque, Unidade Acadêmica de Serra Talhada , Av. Gregório Ferraz Nogueira, S, Hamurábi Anizio Lins, Rayanne Maria Paula Ribeiro, Leonardo Vieira de Sousa, Aurélio Paes Barros Júnior, and Adriano do Nascimento Simões
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Horticulture ,0404 agricultural biotechnology ,media_common.quotation_subject ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Quality (business) ,04 agricultural and veterinary sciences ,Plant Science ,Cultivar ,Biology ,040401 food science ,Agronomy and Crop Science ,media_common - Published
- 2018
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32. Growth dynamics and accumulation of forage mass of forage cactus clones as affected by meteorological variables and water regime
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Marcelo José da Silva, Cleber Pereira Alves, George do Nascimento Araújo Júnior, Márcio Vieira da Cunha, Luciana Sandra Bastos de Souza, Carlos André Alves de Souza, José Raliuson Inácio Silva, Adriano do Nascimento Simões, Sidney Anderson Teixeira da Costa, Thieres George Freire da Silva, and Alexandre Maniçoba da Rosa Ferraz Jardim
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Irrigation ,Agronomy ,Productivity (ecology) ,Fodder ,Air temperature ,Cactus ,Soil Science ,Forage ,Plant Science ,Biology ,Canonical correlation ,Path analysis (statistics) ,Agronomy and Crop Science - Abstract
Comprehending the relation between climatic variables and the productive and morphological characteristics of the forage cactus is essential to the adjustment of the management of its production in different agro-climatic areas. The aim of this study was to evaluate the interrelationship between the meteorological variables and the water regime with the growth dynamics and accumulation of forage mass of three different forage cactus clones. We submitted the clones Miuda–MIU; Orelha de Elefante Mexicana–OEM and IPA Sertânia–IPA to three water regimes (40 %ETc, 80 %ETc and 120 %ETc), plus the non-irrigated condition (0 %ETc), during 18 months, in the municipality of Serra Talhada, Pernambuco, Brazil. Morphological, structural, and productive characteristics of the plants, and environmental variables were supervised throughout the experimental period. Pearson’s correlation, multicollinearity test, canonical correlation and path analysis were applied to these data. It was observed that the minimum air temperature, the relative air humidity, and the irrigation were the variables that affected the growth dynamics and the accumulation of fodder mass of the forage cactus clones the most. The clone OEM presented more sensitivity to the environmental conditions (climate and irrigation) in comparison to the other clones MIU and IPA. The environmental conditions affected the growth dynamics more than the productivity of the clone from the genus Nopalea. It was determined that the species of forage cactus presented differentiated morphological and productive responses among themselves, under semiarid conditions in Brazil.
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- 2021
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33. Inoculation of plant-growth-promoting rhizobacteria in Myracrodruon urundeuva Allemão supports in tolerance to drought stress
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Nathalia Bandeira Diniz, Adriano do Nascimento Simões, Sergio Luiz Ferreira da Silva, Douglas Moreira de Oliveira, André Luiz Alves de Lima, and Carolina Etienne de Rosália e Silva Santos
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0106 biological sciences ,Drought stress ,Azospirillum lipoferum ,semi-arid ,Plant Science ,Aroeira do Sertão tree ,Biology ,lcsh:Plant culture ,Rhizobacteria ,01 natural sciences ,oxidative stress ,Anacardiaceae ,lcsh:SB1-1110 ,Ecology, Evolution, Behavior and Systematics ,Myracrodruon urundeuva ,Inoculation ,fungi ,food and beverages ,04 agricultural and veterinary sciences ,lcsh:QK900-989 ,biology.organism_classification ,Horticulture ,membrane damage ,PGPR ,040103 agronomy & agriculture ,lcsh:Plant ecology ,0401 agriculture, forestry, and fisheries ,010606 plant biology & botany - Abstract
This study evaluated the influence of Azospirillum lipoferum on the growth of Myracroduon urundeuva (Anacardiaceae) plants under drought stress, by means of biometric, physical–chemical and biochemical parameters. The association of A. lipoferum with the roots of the plants provided increases of 30% root length, 50% root dry weight, 34% shoot dry weight and 10% soluble protein content. The inoculated plants still maintained 5% higher leaf water potential than those not inoculated and lower membrane damage. Furthermore, the inoculated plants shown less leaf fall and dark green leaves, confirmed by maintenance of the highest levels of chlorophyl a, b and total. On the other hand, superoxide dismutase activity was significantly lower in the inoculated plants, possibly due to the induction of a non-enzymatic protective feature. In this way, the inoculation of PGPR in M. urundeuva can be an alternative for the production of plants that are more tolerant to drought stress.
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- 2018
34. Changes in the oxidative metabolism of cassava (Manihot esculenta Crantz) roots associated with cultivation managements
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Aurélio Paes Barros Júnior, Moab Torres de Andrade, Domingos Ferreira de Mélo Neto, Daniel Gomes Coelho, Adriano do Nascimento Simões, and Luiz Ferreira Coelho Júnior
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chemistry.chemical_classification ,biology ,Chemistry ,05 social sciences ,050301 education ,04 agricultural and veterinary sciences ,Plant Science ,Metabolism ,040401 food science ,Enzyme assay ,Superoxide dismutase ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Enzyme ,Biochemistry ,Catalase ,biology.protein ,Phenol ,Catechol oxidase ,0503 education ,Agronomy and Crop Science ,Peroxidase - Published
- 2017
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35. APPLICATION OF ANTIOXIDANTS AND EDIBLE STARCH COATING TO REDUCE BROWNING OF MINIMALLY-PROCESSED CASSAVA
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Sérgio Luiz Ferreira-Silva, Daniel Gomes Coelho, Moab Torres de Andrade, Domingos Ferreira de Mélo Neto, and Adriano do Nascimento Simões
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Manihot esculenta Crantz. Alterações fisiológicas. Pós-colheita ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Starch ,Browning ,food and beverages ,04 agricultural and veterinary sciences ,Food science ,General Agricultural and Biological Sciences ,040401 food science ,Manihot esculenta Crantz. Physiological changes. Postharvest - Abstract
This study aimed to evaluate the quality of minimally-processed cassava treated with antioxidants and a starch-based edible coating. Cassava roots were washed, cooled, immersed in cold water, peeled and then cut. Root pieces were then immersed in a chloride solution, centrifuged, and subsequently immersed in either a starch suspension (3%), a solution containing antioxidants (3% citric acid and 3% ascorbic acid), or in both the coating and antioxidant solutions. Coated root pieces were dried at 18 ± 2°C for 1 hour, then packaged into polypropylene bags (150 g per pack) and kept at 5 ± 2°C for 15 days, and assessed every 3 days. A completely randomized design was used in a 4 × 6 factorial consisting of the treatment (control, coating, antioxidant, or coating and antioxidant) and the storage period (0, 3 6, 9, 12 or 15 days), with three replicates in each group. The pH, blackened area and peroxidase and polyphenol oxidase activities of the cassava was reduced in treatments containing antioxidants and the scores of visual analysis and phenolic content were higher. Therefore, treatment with antioxidants was effective for reducing browning in minimally-processed cassava, retaining the quality of cassava pieces stored for 15 days at 5 ± 2°C. The combination of antioxidants and the edible coating showed no improvement compared to treatment with antioxidants alone. Objetivou-se avaliar a qualidade de mandioca de mesa minimamente processada com o uso de antioxidantes e revestimento comestível a base de amido. Raízes de mandioca de mesa foram lavadas, resfriadas, imersas em água fria, cortadas e descascadas. Os pedaços foram imersos em solução clorada, centrifugados e imersos em suspensão de amido (3%) ou em solução contendo antioxidantes (ácido cítrico 3% e ácido ascórbico 3%) ou em ambos (revestimento + antioxidantes). Os pedaços contendo revestimento foram secos em sala climatizada a 18 ± 2 °C por 1 hora. Os pedaços foram embalados em polipropileno, contendo 150 g por embalagem, e mantidos a 5 ± 2 °C por 15 dias, com avaliações a cada três dias. Foi utilizado o DIC, em esquema fatorial 4 x 6 [controle, revestimento, antioxidantes e revestimento + antioxidantes x dias de conservação (0; 3 6; 9; 12 e 15 dias)] com 3 repetições. Verificou-se que os valores de pH, área escurecida e as atividades da peroxidase e polifenoloxidase foram menores e as notas da análise visual e o teor de fenólicos totais foram superiores nos tratamentos contendo antioxidantes. O uso dos antioxidantes foi eficiente na redução do escurecimento em mandioca de mesa minimamente processada, mantendo os pedaços com qualidade por 15 dias a 5 ± 2 ºC e a associação de antioxidantes e revestimento comestível não apresentou melhorias em comparação aos antioxidantes isolados.
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- 2017
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36. Participation of cytokinin on gas exchange and antioxidant enzymes activities
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Damiana Cleuma de Medeiros, Arthur A. S. de Oliveira, Marcos Ribeiro da Silva Vieira, Sergio Luiz Ferreira da Silva, Reinaldo de Alencar Paes, Antônio Henrique Cardoso do Nascimento, Fabiana B. de Moura, Adriano do Nascimento Simões, and Walter Santos Evangelista Júnior
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0106 biological sciences ,0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Plant Science ,Biology ,Photosynthesis ,medicine.disease_cause ,01 natural sciences ,Oxidative damage ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,chemistry.chemical_classification ,fungi ,food and beverages ,Plant physiology ,Enzyme assay ,030104 developmental biology ,Enzyme ,chemistry ,Biochemistry ,Cytokinin ,biology.protein ,Agronomy and Crop Science ,Oxidative stress ,010606 plant biology & botany - Abstract
This review article is aimed to evaluate the effects of foliar application of cytokinin on enzyme activity and photosynthetic parameters. The results obtained on gas exchange and photochemical activity may be related to a standard mechanism of activation defense under specific environments. Further on the basis of study of enzyme activity it can not be stated that cytokinin have effective role in the delay of oxidative damage as contradictory results have been observed by various workers.
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- 2017
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37. Impact of GA3 and spermine on postharvest quality of anthurium cut flowers (Anthurium andraeanum) cv. Arizona
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Marcos Ribeiro da Silva Vieira, Sérgio Luiz Ferreira-Silva, Igor Otavio Minatel, Adriano do Nascimento Simões, Nathalia Bandeira Diniz, Giuseppina Pace Pereira Lima, Monica Bartira da Silva, Federal Rural University of Pernambuco, Universidade Federal do Pará (UFPA), and Universidade Estadual Paulista (Unesp)
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0106 biological sciences ,0301 basic medicine ,Anthurium ,Storage commercial quality ,biology ,Polyphenol oxidase ,Chemistry ,Vase life ,Spermine ,Cut flowers ,Superoxide dismutase ,Horticulture ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Inflorescence ,Postharvest ,Anthurium andraeanum ,Polyamines ,Gibberellic acid ,010606 plant biology & botany ,Peroxidase - Abstract
Made available in DSpace on 2018-12-11T17:37:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-11-18 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco Anthurium cut flowers exposed to low temperatures may be subjected to chilling injury, whereas higher temperatures may accelerate their metabolism and induce premature senescence. Plant growth regulators, as gibberellic acid (GA3) and spermine (SPM), have been described to extend the postharvest life of flowers. In this study, both compounds were applied by spraying or pulsing in anthurium cv. Arizona before storage at 20 °C. The solutions were constituted of 144 μM GA3 and 2 μM SPM, which were used separately or in combination, and analyzed for 12 d. Spraying with GA3 + SPM extended the vase life and kept the commercial quality. These treatments increase the phenols content, as well as, the activity of polyphenol oxidase (PPO), peroxidase (POD), and superoxide dismutase (SOD). Spadix sprayed with GA3 or SPM retained high amounts of spermidine (SPD), and in the combination of GA3 + SPM, there were higher contents of spermidine. These results suggest that the application of GA3 + SPM by spraying can be used to reduce the senescence in anthurium cut flowers stored at 20 °C, and improve the commercial quality of the inflorescences. Federal Rural University of Pernambuco College of Agronomic Engineering Universidade Federal do Pará Department of Chemistry and Biochemistry IB São Paulo State University - UNESP Department of Chemistry and Biochemistry IB São Paulo State University - UNESP CNPq: 305177/2015-0 Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco: APQ-0431-5.01/13
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- 2018
38. Association of preharvest management with oxidative protection and enzymatic browning in minimally processed cassava
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Adriano do Nascimento Simões, Daniel Gomes Coelho, Sérgio Luiz Ferreira-Silva, Luiz Ferreira Coelho Júnior, Moab Torres de Andrade, Domingos Ferreira de Mélo Neto, and Kelem Silva Fonseca
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Manihot ,030309 nutrition & dietetics ,Food Handling ,Biophysics ,Color ,Plant Roots ,Superoxide dismutase ,03 medical and health sciences ,0404 agricultural biotechnology ,Browning ,Peroxidase ,Plant Proteins ,Pharmacology ,0303 health sciences ,biology ,Superoxide Dismutase ,food and beverages ,Sowing ,04 agricultural and veterinary sciences ,Cell Biology ,Catalase ,040401 food science ,Horticulture ,Point of delivery ,biology.protein ,Postharvest ,Preharvest ,Oxidation-Reduction ,Food Science - Abstract
The aim of this study was to examine oxidative protection and enzymatic browning in the storage of minimally processed cassava and their relationship with population density and harvest age. Population densities were 1.0, 1.25, 1.5, and 1.75 plants m-2 . After being harvested at 300, 360, or 420 days after planting, cassava were minimally processed and stored at 5 ± 2°C. It was observed that superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) play key roles in the tolerance of young roots to browning. Planting density, however, does not appear to be a key factor modulating the activity of the enzymes studied. PRACTICAL APPLICATIONS: Younger harvested cassava roots, harvested at 300 days, are more tolerant to enzymatic browning. This appears to be in part due to enzymatic activity modulation of the SOD, CAT, and POD enzymes. In addition, it has been demonstrated that agronomic techniques aimed at increasing productivity, such as increasing the planting density of cassava, do not alter the biomarkers of postharvest quality. In summary, evidence that field management may be an efficient approach to improving the conservation of minimally processed cassava is provided. We believe that the findings of this paper will be of great interest regarding the influence of field management on the postharvest quality of freshly cut cassava and will also provide applicable results relating to its production chain.
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- 2018
39. Darkening, damage and oxidative protection are stimulated in tissues closer to the yam cut, attenuated or not by the environment
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Sérgio Luiz Ferreira-Silva, Marcos Ribeiro da Silva Vieira, Luiz Ferreira Coelho Júnior, Marcelo Augusto Gutierrez Carnelossi, and Adriano do Nascimento Simões
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030309 nutrition & dietetics ,Color ,Oxidative phosphorylation ,Polyphenol oxidase ,Lipid peroxidation ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Ascorbate Peroxidases ,Phenols ,Browning ,TBARS ,Food science ,Plant Proteins ,0303 health sciences ,Nutrition and Dietetics ,biology ,Dioscorea ,Superoxide Dismutase ,Temperature ,04 agricultural and veterinary sciences ,Catalase ,040401 food science ,Plant Tubers ,chemistry ,Food Storage ,biology.protein ,Agronomy and Crop Science ,Oxidation-Reduction ,Catechol Oxidase ,Food Science ,Biotechnology ,Peroxidase - Abstract
This study investigated how the activities of the enzymes and metabolites of oxidative metabolism are affected in different regions of cut tissue, associating these changes with the evolution of browning in fresh-cut yam. Samples were collected from yam at 0?5 and 5?10?mm from the cut site. Fresh-cut yams were stored at 5 or 26?°C for days or hours respectively, simulating commercialization, with or without packaging. RESULTS The results demonstrated that the injury to the yam was a physical inducer of changes in the levels of lipid peroxidation and phenolic compounds and in the activities of superoxide dismutase, catalase, ascorbate peroxidase, polyphenol oxidase and peroxidase. These responses were significant in the tissue closest to the wound but also observed, although less intense, in the more distant tissue. The combined effects of wounding and dehydration during storage intensified the above responses. Conversely, refrigeration attenuated the transmission of the wounding response through the adjacent tissue. CONCLUSION The results of this work provide the first evidence in cut yam roots that the membrane degradation products, enzymes involved in oxidative protection and enzymes that modulate the oxidation of phenolic compounds are intertwined mechanisms that cause tissue darkening. ? 2018 Society of Chemical Industry
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- 2018
40. QUALIDADE DO INHAME (Dioscorea sp.) MINIMAMENTE PROCESSADO CONSERVADO EM DUAS TEMPERATURAS
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Clarissa Soares Freire, Sérgio Luiz Ferreira-Silva, Adriano do Nascimento Simões, Edson Fábio da Silva, and Aurélio Paes Barros Júnior
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Atividade enzimática. Conservação pós-colheita. Dioscorea sp.. Pseudomonas sp ,Enzymatic activity ,biology ,Chemistry ,lcsh:S ,04 agricultural and veterinary sciences ,biology.organism_classification ,Conservação pós-colheita ,040401 food science ,Post-harvest conservation ,lcsh:Agriculture ,Dioscorea sp ,Atividade enzimática ,Horticulture ,Enzymatic activity. Post-harvest conservation. Dioscorea sp.. Pseudomonas sp ,0404 agricultural biotechnology ,lcsh:Biology (General) ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Pseudomonas sp ,Dioscorea ,General Agricultural and Biological Sciences ,lcsh:QH301-705.5 - Abstract
This work studied the physical, chemical and biochemical alterations in minimally processed yam stored at two different temperatures, as well as the incidence of bacteria of the genus Pseudomonas. The experimental design was completely randomised in a 2x8 factorial design, with two storage temperatures (5 and 10°C) and eight storage times (0, 2, 4, 6, 8, 10, 12 and 14 days). Experiments were in triplicate. Yam was selected, peeled and cut into slices of approximately 3 cm thickness. The slices were rinsed with water, sanitised and then drained in kitchen strainers. Approximately 300 g of the processed product were packed in nylon multilayers 15 µm thick, 15 cm wide and 20 cm long. The packs were sealed, weighed and kept at 5 and 10 ± 2°C for 14 days. Fresh weight loss, baking time, enzymatic activity of polyphenol oxidases, peroxidases and catalases, total soluble phenol content, and antioxidant capacity were evaluated, as well as visual analysis and incidence of Pseudomonas sp. Means of temperatures were compared by Tukey´s test at 5% significance. Yam storage at 5°C reduced weight loss and kept visual quality for longer; it also reduced cooking time and the activity of the enzymes polyphenol oxidase and peroxidase. In contrast, it promoted higher content of total soluble phenols, as well as a higher catalase activity and antioxidant capacity. During the storage time, there was no incidence of Pseudomonas sp. Minimally processed yam stored at 10°C may be sold for up to six days, and yam stored at 5ºC for up to 14 days. RESUMO: O objetivo deste trabalho foi estudar as alterações físico-químicas, bioquímicas e incidência de Pseudomonas sp. em inhame minimamente processado conservado em duas temperaturas. O delineamento experimental utilizado foi o inteiramente casualizado em esquema fatorial 2x8, referente as temperaturas (5 e 10 °C) e tempos de conservação refrigerada (0, 2, 4, 6, 8, 10, 12 e 14 dias), com três repetições. O inhame foi selecionado, descascado e cortado em rodelas de aproximadamente 3 cm de espessura. Essas rodelas foram enxaguadas em água, sanitizadas e drenadas em escorredores de cozinha. Aproximadamente 300 g do produto processado foram embalados em Nylon multicamadas de 15 µm de espessura, com 15 cm de largura por 20 cm de comprimento. As embalagens foram seladas, pesadas e mantidas a 5 e 10 ± 2 °C por 14 dias. Avaliou-se a perda de massa, tempo de cocção, atividade enzimática das polifenoloxidases e peroxidases, conteúdo de fenóis solúveis totais, capacidade antioxidante, análise visual e incidência de Pseudomonas sp. As médias entre as temperaturas foram comparadas pelo teste de Tukey a 5 % de significância. A temperatura de 5 °C reduziu a perda de massa fresca e da qualidade visual, o tempo de cocção e a atividade das enzimas polifenoloxidases e peroxidases. Por outro lado, proporcionou maior conteúdo de fenóis solúveis totais e capacidade antioxidante. Ao longo da conservação, não foi observada incidência de Pseudomonas sp. O inhame minimamente processado, sob temperatura de 10 °C, pode ser comercializado por até 6 dias. A 5º C, por até 14 dias.
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- 2016
41. Baby Cassava: An Alternative Marketing Strategy for Freshly Cut Cassava
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Kelem Silva Fonseca, Domingos Ferreira de MeloNeto, Rainério Meireles da Silva, Aline Ellen Duarte de Sousa, Adriano do Nascimento Simões, Fred Augusto Lourêdo de Brito, Moab Torres de Andrade, and Daniel GomesCoelho
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0106 biological sciences ,Agricultural science ,business.industry ,04 agricultural and veterinary sciences ,Business ,0405 other agricultural sciences ,01 natural sciences ,Marketing strategy ,040501 horticulture ,010606 plant biology & botany - Published
- 2018
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42. Quality of Melon 'UFERSA-05 var. ACIDULUS and Pele de Sapo 'Mabel' Minimally Processed, Kept in Different Packages
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Maria Luiza Pereira de Araújo, Adriano do Nascimento Simões, Pahlevi Augusto de Souza, Fanquiele Ribeiro de Oliveira, Marianne Lima Barboza, and Glauber Enrique Souza Nunes
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Horticulture ,PME activity ,biology ,Soluble solids ,Chemistry ,Melon ,Flesh ,Botany ,Titratable acid ,biology.organism_classification ,Flavor ,Aroma - Abstract
This study aimed at evaluating the quality of melon Pele de Sapo “Mabel” and Yellow melon “UFERSA-05” minimally processed preserved in different packages. The fruits were harvested at Mossoro-RN and transported to Serra Talhada-PE. In the first study, melon fruit Piel de Sapo “Mabel” was selected, washed, weighed, cooled, peeled, cut into cube shape, sanitized in chlorine solution, drained, packaged in rigid polypropylene tray, sealed with polypropylene film or polypropylene rigid cap and kept for 8 days at 8℃. In the second study, fruits of yellow melon “UFERSA-05” and Pele de Sapo “Mabel” were minimally processed and kept in rigid polypropylene tray, sealed with polypropylene film and stored for 8 days at 8℃. There was no significant interaction between packaging (sealed with film and polypropylene cover) and period of conservation, for pH, total titratable acidity (TTA), total soluble solids (TSS) and Loss of fresh mass (LFM) for Melon “Mabel”. While there was significant interaction between types of melon (“UFERSA-05” and “Mabel”) and storage period (0, 2, 4, 6, and 8 days) for pH, TTA and TSS. In sensory evaluation were noticeable changes in appearance, flavor, aroma and flesh firmness, characterized by translucency, alcoholic aroma and softening in “Mabel” melon kept in tray with lid. Melon “UFERSA-05” showed lower pH, total soluble solids, total soluble sugars, PME activity, lack of translucency high flesh firmness compared to “Mabel” melon during storage. The high levels of sugars in melon “Mabel” may be related to the incidence of translucency, which was not observed in “UFERSA-05” melon, with a strong potential to minimal processing.
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- 2014
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43. Effect of low temperature storage on conservation varieties of Chrysanthemum cutting
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Adriano do Nascimento Simões, Glauber Henrique, Pahlevi Augusto de Souza, and Marcos Ribeiro da Silva
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Horticulture ,Inflorescence ,Agronomy ,Dendranthema grandiflora ,Postharvest ,Greenhouse ,Biology - Abstract
The objective of this research was to evaluate postharvest quality of ‘Lona’ and ‘Garfield’ varieties chrysanthemums, stored at different temperatures. The experiment was carried out in a plastic greenhouse at Pouso Alegre, Minas Gerais State, Brazil (22° 13'48" S, 45° 56'11" W and 832 m in height). The inflorescences were kept at 1.5, 2.5 and 5.0°C. The evaluated parameters were senescent flowers and necrosed ligules. The evaluations were performed in the open storage room at 4, 8, and 12 days, at room temperature. It was observed that chrysanthemum ‘Lona’ flower senescence was accelerated at 2.5 and 5.0°C; while for ‘Garfield’, the senescence was larger at 1.5°C. For ‘Lona’ and ‘Garfield’ chrysanthemums, the temperature of 1.5°C favored the development of necrosis. Key words: Dendranthema grandiflora Tzvelev, varieties, pompom, conservation.
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- 2013
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44. Quality of three cowpea green-grains cultivars refrigerated
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Adriano do Nascimento Simões, Maria Cristina Tavares de Albuquerque, José Ricardo Tavares de Albuquerque, Hamurábi Anizio Lins, Aurélio Paes Barros Júnior, and Edson Fábio da Silva
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Horticulture ,media_common.quotation_subject ,General Earth and Planetary Sciences ,Quality (business) ,Cultivar ,Business ,General Environmental Science ,media_common - Published
- 2017
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45. Populational density and harvest age of carrots for baby carrot manufacture
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Alysson Roberto de Almeida, Ricardo Hs Santos, Rolf Puschmann, Franciscleudo Bezerra da Costa, Silvino Intra Moreira, and Adriano do Nascimento Simões
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chemistry.chemical_classification ,sistema de plantio ,Crop yield ,Randomized block design ,food and beverages ,Soil Science ,Sowing ,Plant Science ,Orange (colour) ,Horticulture ,Raw material ,Biology ,biology.organism_classification ,minimal processing ,Population density ,Daucus carota ,processamento mínimo ,planting system ,Agronomy ,chemistry ,quality ,Carotenoid ,qualidade - Abstract
The quality of baby carrots may be associated to the standard of the raw material that in turn, may be influenced by agronomic practices. Thus, the effect of planting density and harvest age were assessed on physical and morphological characteristics, yield and end quality of intact and minimally processed carrots in the form of baby carrots. A randomized block experimental design was used with the density in the plots (4 x 20 cm and 4 x 7.5 cm) and the harvest age in the subplots (50, 57, 64, 71, 78, 85, 92, 99 and 106 days), with five replications. The experimental unit consisted of 25 plants. The assessments of diameter, length, yield, secondary phloem translocation with the cambio and total carotenoids were decisive in selecting the carrot harvest age for baby carrot manufacture. Under the 4 x 20 cm and 4 x 7.5 cm adensed conditions, harvested between 85 and 92 days, carrot roots became thinner and more orange in color (with maximum carotenoid accumulation), larger and with satisfactory yields and therefore more acceptable for minimal processing in the form of baby carrots. Thus the decision of populational density associated to harvest age will depend on the market demand, on price, quality and other variables. The use of adensed cropping with early harvests may lead to obtain carrots and respective baby carrots that are more acceptable for commercialization. A qualidade final de minicenoura pode estar associada ao padrão da matéria prima, que, por sua vez, pode ser influenciada por práticas agronômicas. Por isso, objetivou-se avaliar o efeito da densidade de plantio e da idade de colheita em características físicas, morfológicas, no rendimento e na qualidade final de cenoura inteira e minimamente processada na forma de minicenoura. O delineamento experimental utilizado foi em blocos casualizado, tendo nas parcelas as densidades (4 x 20 cm e 4 x 7,5 cm) e nas subparcelas a idade de colheita (50, 57, 64, 71, 78, 85, 92, 99 e 106 dias), com 5 repetições onde a unidade experimental foi composta por 25 plantas. As características de diâmetro, comprimento, rendimento, descolamento do floema secundário com o câmbio e carotenóides totais foram decisivos para selecionar a idade de colheita de cenoura destinada à fabricação de minicenoura. Nos dois adensamentos estudados, as raízes colhidas entre 85 e 92 dias, tornaram-se mais finas, de cor mais laranja (com máximo acúmulo de carotenóides), maiores, com rendimentos satisfatórios e, portanto, mais aceitáveis ao processamento mínimo na forma de minicenoura. Logo, a decisão de uso de densidade populacional associado à idade para se realizar a colheita, dependerá da exigência do mercado, do preço, qualidade e de outras variáveis. O uso de adensamentos de cultivo associados com colheitas antecipadas pode proporcionar a obtenção de cenoura e respectivas minicenouras mais aceitáveis para comercialização.
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- 2010
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46. Anatomical and physiological evidence of white blush on baby carrot surfaces
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Marília Contin Ventrella, Rolf Puschmann, Adriano do Nascimento Simões, Celso Luiz Moretti, and Marcelo Augusto Gutierrez Carnelossi
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biology ,Chemistry ,PAL activity ,food and beverages ,Orange (colour) ,Horticulture ,biology.organism_classification ,medicine.disease ,Sensory analysis ,Instrumental evaluation ,Botany ,Visual assessment ,medicine ,Food science ,Dehydration ,Agronomy and Crop Science ,Legume ,Food Science ,Daucus carota - Abstract
This study characterizes dehydration and white blush processes, structural alterations and synthesis of phenolic compounds (lignin and suberin), in relation to development of white blush on baby carrot surfaces. Carrots were minimally processed as baby carrots and kept on polypropylene trays with or without polyvinyl chloride (PVC) film at 5 ± 2 °C, 90 ± 5% RH. During storage, baby carrots that were not wrapped with PVC film were rehydrated 1, 1.5, 15 and 17 h after minimal processing. Fresh-cut baby carrots were evaluated for white blush index, sensory analysis (visual scores), fresh matter loss, phenylalanine ammonia-lyase (PAL) activity and structural and histochemical changes. Increases in white blush index and subjective visual scores on the carrot surface occurred in the first hours, when the material was kept on trays without PVC film and after 3 and 6 d, when stored on trays covered with PVC film. Visual assessment of white blush resulted in a more accurate assessment than instrumental evaluation because it allowed the perception of minor differences between distinct white blush levels, especially at the tips. Hydrating baby carrot surfaces for 1 and 1.5 h after processing allowed partial absorption of water by tissues and the orange color was reestablished on the surface. Fifteen hours after processing, even after rehydration, the color did not return to the original orange standard. The rapid increase in PAL activity in the secondary phloem of baby carrots, compared to that of whole carrots, suggested a physiological response as a result of abrasion. Dehydration and structural alterations of the more superficial cell layers were the main causes of white blush in baby carrots that was not related to lignin accumulation, but rather to synthesis of non-structural phenolic compounds.
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- 2010
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47. Changes in phenol metabolism of minimally processed baby cassava under different temperatures: An alternative to commercialization
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Fred, Augusto Lourêdo de Brito, primary, Rúbia, Kécia Marins Maia, additional, Luiz, Ferreira Coelho Júnior, additional, Daniel, Gomes Coelho, additional, Marcos, Ribeiro da Silva Vieira, additional, Sérgio, Luiz Ferreira da Silva, additional, and Adriano, do Nascimento Simões, additional
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- 2017
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48. Armazenamento de melão amarelo, híbrido RX 20094, sob temperatura ambiente
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Josivan Barbosa Menezes, Fábio Vinícius de S. Mendonça, Pahlevi Augusto de Souza, Adriano do Nascimento Simões, Adriana A. Guimarães, and Georgiana L. F. M. Souza
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Melon ,Flesh ,Soil Science ,Mineralogy ,Plant Science ,Horticulture ,Cucumis melo L ,armazenamento ,Soluble solids ,Postharvest ,Relative humidity ,Quality characteristics ,pós-colheita ,Completely randomized design ,qualidade ,Mathematics - Abstract
A vida útil pós-colheita do melão amarelo híbrido RX 20094 foi avaliada, estudando-se características visuais, físicas e físico-químicas dos frutos em pós-colheita em ensaio conduzido no laboratório da Escola Superior de Agricultura de Mossoró. Utilizou-se delineamento inteiramente casualizado com seis tratamentos, referentes aos períodos de armazenamento (0; 5; 10; 15; 20; e 25 dias) e 5 repetições, com dois frutos por parcela. Os frutos foram mantidos à temperatura de 30±1ºC e UR de 50±5%. Foram feitas avaliações em intervalos de 5 dias, avaliando-se a firmeza da polpa, perda de massa, aparências externa e interna e conteúdo de sólidos solúveis (SS). Observou-se efeito do tempo de armazenamento para todas as características avaliadas. A firmeza da polpa e as aparências externa e interna foram os principais fatores responsáveis pela perda de qualidade do melão. A vida útil do híbrido RX-20094 foi estimada em até no mínimo 25 dias de armazenamento sob temperatura ambiente.
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- 2004
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49. Endogenous levels of polyamines during cold storage of bird-ofparadise treated with biocides
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Giuseppina Pace Pereira Lima, Luiza Maria de Souza Fernandes, Marcos Ribeiro da Silva Vieira, Angela Vacaro de Souza, Pahlevi Augusto de Souza, Armando Reis Tavares, Reginaldo Conceição Cerqueira, and Adriano do Nascimento Simões
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Bract ,fungi ,food and beverages ,Spermine ,Cold storage ,chemistry.chemical_element ,Plant Science ,lcsh:Plant culture ,Horticulture ,Strelitzia reginae, inflorescence, ornamental plant, postharvest, senescence ,Spermidine ,chemistry.chemical_compound ,chemistry ,Ornamental plant ,Putrescine ,Chlorine ,Postharvest ,lcsh:SB1-1110 - Abstract
This study aimed to analyse the endogenous levels of free polyamines putrescine (Put), spermidine (spd) and spermine (spm) under the effect of 8-HQC (8-hydroxyquinoline citrate) and chlorine during cold storage of bird-of-paradise floral scapes. Flowers were selected, labelled and randomly distributed in recipients for postharvest trials. The base of flower scapes was immersed with pulsing treatments (Control, 100 chlorine, 250 or 500 mg L-1 8-HQC) for 48 h, thereafter, stored at 10.5 °C and 90% relative humidity for a period of 12 days. Flower parts were sampled for polyamines analysis at day 0, 4, 8 and 12 days during storage. All samples had higher Put levels than the control. Treatment with 500 mg L-1 8-HQC showed the highest Spd levels in bracts, while chlorine treatment had the highest Spm levels in stems.
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- 2017
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50. Influence of storage temperature on quality of inflorescences strelitzia (strelitzia reginae banks ex aiton)
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Marcos Ribeiro da Silva Vieira, Giuseppina Pace Pereira Lima, Angela Vacaro de Souza, Nelson Geraldo de Oliveira, Adriano do Nascimento Simões, Universidade Estadual Paulista (Unesp), and Universidade Federal de Pernambuco (UFPE)
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Agricultural Sciences ,QH301-705.5 ,Conservação ,strelitzia reginae ,Agriculture ,Flowers ,Conservation ,flores ,Senescence ,senescência ,Flores ,Senescência ,Strelitzia reginae ,conservação ,Biology (General) - Abstract
Made available in DSpace on 2015-11-03T15:28:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01. Added 1 bitstream(s) on 2015-11-04T10:15:14Z : No. of bitstreams: 1 WOS000350408000034.pdf: 811327 bytes, checksum: 9c4040edf6e431d323de54713a4b5cf2 (MD5) O presente trabalho tem por objetivo de estudar a influência da temperatura de armazenamento (câmara fria e temperatura ambiente) na qualidade das inflorescências de estrelítzia. Escapos florais foram selecionados, etiquetados e descartados quanto à presença de danos mecânicos, doenças e/ou pragas. Transcorrido esse período, foram transferidos para recipientes, onde foram submetidos a dois experimentos. No experimento 1, escapos foram colocados em baldes contendo água da rede pública e levadas para câmara fria a 7,5 ºC e UR de 90%, por um período de doze dias. No experimento 2, os escapos foram mantidos nas mesmas condições, porém em temperatura ambiente por um período de seis dias. Nos dois experimentos, as análises visuais: coloração, brilho, manchas (através da atribuição de notas), abertura e queda de floretes (contagem) foram avaliadas em intervalo de quatro dias em câmara fria e a cada 48 horas em condições de temperatura ambiente. A sépala foi o órgão que apresentou maior perda na coloração. A variável brilho apresentou o mesmo comportamento em relação aos dois experimentos. Maiores incidências de manchas nas inflorescências ocorreram em temperatura ambiente. Os escapos apresentaram aumento no número de floretes abertos em câmara fria. Essa tendência não ocorreu em temperatura ambiente. Não foram observados diferenças na queda de floretes. Conclui-se que a temperatura de armazenamento não contribui para a qualidade pós-colheita de estrelítzia. This work was based to study the influence of the storage temperature (cold and room temperature) in the quality of inflorescences strelitzia. The scapes were selected, labeled and there were zero problems concerning mechanical damage, disease and/or plagues. Subsequently this period, the scapes were moved randomly to recipients with water, in which two postharvest trials were conducted. In experiment 1, the flower scapes were placed in buckets with water from public supply and sanitation department and taken to a cold room at temperature of 7.5 degrees C and RH of 90%, for a twelve day period. For the experiment 2, were kept under the same conditions but at room temperature for a period of six days. In both experiments, the visual analysis: color, gloss, stains (by assigning notes), opening and drop florets (count) were evaluated at intervals of four days in cold and every 48 hours at ambient temperature conditions. In both experiments, the visual analysis: color, gloss, stains (by assigning notes), opening and drop florets (count) were evaluated at intervals of four days in cold and every 48 hours at ambient temperature conditions. The sepal is the organ that showed greater loss in coloration. The variable gloss showed the same pattern for the two experiments. Incidences of stains on the inflorescences occurred in patches at room temperature. The scapes increased number of florets open in cold. This tendency did not occur at room temperature. No were observed differences in the fall of florets. Conclude that the storage temperature does not contribute to postharvest quality of strelitzia. Univ Estadual Paulista, Dept Quim &Bioquim, Inst Biociencias, Botucatu, SP, Brazil Univ Estadual Paulista, Dept Prod Vegetal, Botucatu, SP, Brazil Univ Fed Rural Pernambuco, Unidade Acad Serra Talhada, Serra Talhada, PE, Brazil Univ Estadual Paulista, Dept Quim &Bioquim, Inst Biociencias, Botucatu, SP, Brazil Univ Estadual Paulista, Dept Prod Vegetal, Botucatu, SP, Brazil
- Published
- 2014
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