24 results on '"Achromobacter spp"'
Search Results
2. Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene
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Filipić, Brankica, Malešević, Milka, Vasiljević, Zorica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, Filipić, Brankica, Malešević, Milka, Vasiljević, Zorica, Novović, Katarina, Kojić, Milan, and Jovčić, Branko
- Abstract
Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.
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- 2022
3. Diversidad de las especies de Achromobacter recuperadas de pacientes con fibrosis quística en Argentina
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German Matias Traglia, Gabriel Osvaldo Gutkind, Maria Soledad Ramirez, Pablo Francisco Martina, Carla Steffanowski, Carlos Vay, Laura Galanternik, Marcela Alejandra Radice, Mariana Andrea Papalia, and Marisa Almuzara
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Microbiology (medical) ,Achromobacter ,Cystic Fibrosis ,ACHROMOBACTER SPP ,Argentina ,GENOTYPING ,Microbiology ,Tazobactam ,purl.org/becyt/ford/3.3 [https] ,03 medical and health sciences ,Complete sequence ,Antibiotic resistance ,Drug Resistance, Bacterial ,ANTIBIOTIC RESISTANCE ,medicine ,Humans ,EPIDEMIOLOGY ,Genotyping ,0303 health sciences ,biology ,030306 microbiology ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,Anti-Bacterial Agents ,Phenotype ,Multilocus sequence typing ,purl.org/becyt/ford/3 [https] ,CYSTIC FIBROSIS ,medicine.drug ,Piperacillin - Abstract
Different phenotype-based techniques and molecular tools were used to describe the distribution of different Achromobacter species in patients with cystic fibrosis (CF) in Argentina, and to evaluate their antibiotic resistance profile. Phenotypic identification was performed by conventional biochemical tests, commercial galleries and MALDI-TOF MS. Genetic approaches included the detection of A. xylosoxidans specific marker blaoxa-114, the amplification and sequencing of the 16S rRNA gene, nrdA and blaOXA complete sequence, and MLST analysis. Phenotypic approaches, even MALDI-TOF, rendered inconclusive or misleading results. On the contrary, concordant results were achieved with the nrdA sequencing or sequence type (ST) analysis, and the complete blaOXA sequencing, allowing a reliable discrimination of different Achromobacter species. A. xylosoxidans accounted for 63% of Achromobacter infections and A. ruhlandii accounted for 17%. The remaining species corresponded to A. insuavis, A. dolens, A. marplatensis and A. pulmonis. Antimicrobial susceptibilities were determined by the agar dilution method according to CLSI guidelines. Piperacillin, piperacillin/tazobactam and carbapenems were the most active antibiotics. However, the emergence of carbapenem-resistant isolates was detected. In conclusion, prompt and accurate identification tools were necessary to determine that different Achromobacter species may colonize/infect the airways of patients with CF. Moreover, antimicrobial therapy should be administered based on the susceptibility profile of individual Achromobacter sp. isolates. Se emplearon diversas técnicas fenotípicas y moleculares para describir la distribución de diferentes especies del género Achromobacter en pacientes con fibrosis quística (FQ) en Argentina, y se evaluó el perfil de resistencia a los antibióticos. Se realizó la identificación fenotípica por pruebas bioquímicas convencionales, galerías comerciales y MALDI-TOF MS. El enfoque genético incluyó la detección del marcador especie-específico de A. xylosoxidans blaoxa-114, la amplificación y la secuenciación de los genes ARNr 16S, nrdA y secuencia completa de blaOXA, y el análisis por MLST. Los enfoques fenotípicos, incluso la técnica de MALDI-TOF, proporcionaron resultados no concluyentes o erróneos. Por el contrario, se obtuvieron resultados concordantes entre la secuenciación del gen nrdA o el análisis de secuenciotipos (ST) y la secuenciación completa de blaOXA, lo que permitió una discriminación confiable de las diferentes especies de Achromobacter. A. xylosoxidans representó el 63% de las infecciones por Achromobacter y A. ruhlandii representó el 17%. Las especies restantes correspondieron a A. insuavis, A. dolens, A. marplatensis y A. pulmonis. Se determinó la sensibilidad a antimicrobianos por el método de dilución en agar de acuerdo al CLSI. Los antibióticos más activos fueron piperacilina, piperacilina/tazobactam y carbapenemes. Sin embargo, se detectó la emergencia de aislamientos resistentes a carbapenemes. En conclusión, resultaron necesarias herramientas de identificación rápida y precisas para determinar las diferentes especies del género Achromobacter capaces de colonizar/infectar las vías respiratorias de los pacientes con FQ. Asimismo, la terapia antimicrobiana debería llevarse a cabo en función del perfil de sensibilidad de los aislamientos individuales de Achromobacter spp. Fil: Papalia, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Steffanowski, Carla Giselle. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Almuzara, Marisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina Fil: Martina, Pablo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Galanternik, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina Fil: Vay, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Cátedra de Microbiología; Argentina Fil: Ramírez, María Soledad. California State University; Estados Unidos Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
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- 2020
4. Achromobacter spp. in Cystic Fibrosis Patients: A Genomic-Based Approach to Unravel Microbe-Host Adaptation
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Veschetti, Laura
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cystic fibrosis ,Achromobacter spp ,Settore MED/03 - Genetica Medica ,genomic analysis ,Achromobacter spp, cystic fibrosis, genomic analysis - Published
- 2022
5. Achromobacter spp. Surgical Site Infections: A Systematic Review of Case Reports and Case Series
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Eve Ronin, André Cabié, Karine Marion-Sanchez, and Christian Derancourt
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Microbiology (medical) ,medicine.medical_specialty ,Achromobacter species ,Achromobacter ,biology ,business.industry ,QH301-705.5 ,Digestive surgery ,Similar distribution ,Achromobacter spp ,review ,surgical site infection ,biology.organism_classification ,Microbiology ,immunocompetent ,Virology ,Internal medicine ,Individual data ,Surgical site ,medicine ,Limited evidence ,Biology (General) ,business ,Surgical site infection - Abstract
Achromobacter species are isolated from rare but severe healthcare-associated infections, including surgical site infections. They are considered to preferentially infect immunocompromised patients but so far with limited evidence. We conducted a systematic review on Achromobacter spp. surgical site infections (SSIs) to determine if such infections were indeed more commonly associated with immunocompromised patients. The secondary objective was to describe the characteristics of infected patients. Eligible articles had to be published before 30 September 2020 and to report Achromobacter spp. SSIs across all surgical specialties excluding ophthalmology. Analyses were performed on individual data without meta-analysis. Cases were divided into 2 subgroups: one group which had either prosthesis or implant and the other group which did not. A first selection led to a review of 94 articles, of which 37 were analyzed. All were case reports or case series and corresponded to 49 infected patients. Most of the patients were under 65 years of age and had undergone a heart or digestive surgery followed by deep infection with no co-infecting pathogens. Nine out of the 49 cases were immunocompromised, with similar distribution between the two subgroups (16.6% and 20%, respectively). This review suggests that Achromobacter spp. SSIs do not preferentially target immunocompromised patients.
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- 2021
6. Adaptive interactions of achromobacter spp. With pseudomonas aeruginosa in cystic fibrosis chronic lung co-infection
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Sandri, Angela, Haagensen, Janus Anders Juul, Veschetti, Laura, Johansen, Helle Krogh, Molin, Søren, Malerba, Giovanni, Signoretto, Caterina, Boaretti, Marzia, Lleo, Maria M., Sandri, Angela, Haagensen, Janus Anders Juul, Veschetti, Laura, Johansen, Helle Krogh, Molin, Søren, Malerba, Giovanni, Signoretto, Caterina, Boaretti, Marzia, and Lleo, Maria M.
- Abstract
In the lungs of patients with cystic fibrosis (CF), the main pathogen Pseudomonas aeruginosa is often co-isolated with other microbes, likely engaging in inter-species interactions. In the case of chronic co-infections, this cohabitation can last for a long time and evolve over time, potentially contributing to the clinical outcome. Interactions involving the emerging pathogens Achromobacter spp. have only rarely been studied, reporting inhibition of P. aeruginosa biofilm formation. To evaluate the possible evolution of such interplay, we assessed the ability of Achromobacter spp. isolates to affect the biofilm formation of co-isolated P. aeruginosa strains during long-term chronic co-infections. We observed both competition and cohabitation. An Achromobacter sp. isolate secreted exoproducts interfering with the adhesion ability of a co-isolated P. aeruginosa strain and affected its biofilm formation. Conversely, a clonal Achromobacter sp. strain later isolated from the same patient, as well as two longitudinal strains from another patient, did not show similar competitive behavior against its P. aeruginosa co-isolates. Genetic variants supporting the higher virulence of the competitive Achromobacter sp. isolate were found in its genome. Our results confirm that both inter-species competition and cohabitation are represented during chronic co-infections in CF airways, and evolution of these interplays can happen even at the late stages of chronic infection.
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- 2021
7. Adaptive Interactions of
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Angela, Sandri, Janus Anders Juul, Haagensen, Laura, Veschetti, Helle Krogh, Johansen, Søren, Molin, Giovanni, Malerba, Caterina, Signoretto, Marzia, Boaretti, and Maria M, Lleo
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cystic fibrosis ,lung infection ,Pseudomonas aeruginosa ,Achromobacter spp ,Article ,inter-species interactions - Abstract
In the lungs of patients with cystic fibrosis (CF), the main pathogen Pseudomonas aeruginosa is often co-isolated with other microbes, likely engaging in inter-species interactions. In the case of chronic co-infections, this cohabitation can last for a long time and evolve over time, potentially contributing to the clinical outcome. Interactions involving the emerging pathogens Achromobacter spp. have only rarely been studied, reporting inhibition of P. aeruginosa biofilm formation. To evaluate the possible evolution of such interplay, we assessed the ability of Achromobacter spp. isolates to affect the biofilm formation of co-isolated P. aeruginosa strains during long-term chronic co-infections. We observed both competition and cohabitation. An Achromobacter sp. isolate secreted exoproducts interfering with the adhesion ability of a co-isolated P. aeruginosa strain and affected its biofilm formation. Conversely, a clonal Achromobacter sp. strain later isolated from the same patient, as well as two longitudinal strains from another patient, did not show similar competitive behavior against its P. aeruginosa co-isolates. Genetic variants supporting the higher virulence of the competitive Achromobacter sp. isolate were found in its genome. Our results confirm that both inter-species competition and cohabitation are represented during chronic co-infections in CF airways, and evolution of these interplays can happen even at the late stages of chronic infection.
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- 2021
8. Virulence and antibiotic resistance of Achromobacter spp. isolates from chronic and occasional lung infection in cystic fibrosis patients
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Laura Veschetti, Sandri, Angela, Saitta, Giulia Maria, Passarelli Mantovani, Rebeca, Burlacchini, Gloria, Preato, Sara, Claudio Sorio, Melotti, Paola Maria, Fiscarelli, Ersilia V., Signoretto, Caterina, Boaretti, Marzia, Giovanni Malerba, and Lleo Fernandez, Maria Del Mar
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cystic fibrosis ,virulence ,antibiotic resistance ,Achromobacter spp - Published
- 2021
9. Adaptive Interactions of Achromobacter spp. with Pseudomonas aeruginosa in Cystic Fibrosis Chronic Lung Co-Infection
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Marzia Boaretti, Giovanni Malerba, Helle Krogh Johansen, Maria M. Lleo, Søren Molin, Caterina Signoretto, Laura Veschetti, Janus A. J. Haagensen, and Angela Sandri
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0301 basic medicine ,Microbiology (medical) ,Achromobacter ,030106 microbiology ,Achromobacter spp ,Virulence ,medicine.disease_cause ,Cystic fibrosis ,Microbiology ,cystic fibrosis ,03 medical and health sciences ,Pseudomonas aeruginosa ,inter-species interactions ,lung infection ,medicine ,Immunology and Allergy ,Molecular Biology ,Pathogen ,Lung ,General Immunology and Microbiology ,biology ,Biofilm ,medicine.disease ,biology.organism_classification ,Chronic infection ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Medicine ,Inter-species interactions ,Lung infection - Abstract
In the lungs of patients with cystic fibrosis (CF), the main pathogen Pseudomonas aeruginosa is often co-isolated with other microbes, likely engaging in inter-species interactions. In the case of chronic co-infections, this cohabitation can last for a long time and evolve over time, potentially contributing to the clinical outcome. Interactions involving the emerging pathogens Achromobacter spp. have only rarely been studied, reporting inhibition of P. aeruginosa biofilm formation. To evaluate the possible evolution of such interplay, we assessed the ability of Achromobacter spp. isolates to affect the biofilm formation of co-isolated P. aeruginosa strains during long-term chronic co-infections. We observed both competition and cohabitation. An Achromobacter sp. isolate secreted exoproducts interfering with the adhesion ability of a co-isolated P. aeruginosa strain and affected its biofilm formation. Conversely, a clonal Achromobacter sp. strain later isolated from the same patient, as well as two longitudinal strains from another patient, did not show similar competitive behavior against its P. aeruginosa co-isolates. Genetic variants supporting the higher virulence of the competitive Achromobacter sp. isolate were found in its genome. Our results confirm that both inter-species competition and cohabitation are represented during chronic co-infections in CF airways, and evolution of these interplays can happen even at the late stages of chronic infection.
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- 2021
10. Poorly processed reusable surface disinfection tissue dispensers may be a source of infection.
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Kampf, Günter, Degenhardt, Stina, Lackner, Sibylle, Jesse, Katrin, von Baum, Heike, and Ostermeyer, Christiane
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DISINFECTION & disinfectants , *DECONTAMINATION (From gases, chemicals, etc.) , *SURFACE active agents , *HEALTH facilities , *ACHROMOBACTER , *SERRATIA marcescens - Abstract
Background Reusable surface disinfectant tissue dispensers are used in hospitals in many countries because they allow immediate access to pre-soaked tissues for targeted surface decontamination. On the other hand disinfectant solutions with some active ingredients may get contaminated and cause outbreaks. We determined the frequency of contaminated surface disinfectant solutions in reusable dispensers and the ability of isolates to multiply in different formulations. Methods Reusable tissue dispensers with different surface disinfectants were randomly collected from healthcare facilities. Solutions were investigated for bacterial contamination. The efficacy of two surface disinfectants was determined in suspension tests against two isolated species directly from a contaminated solution or after 5 passages without selection pressure in triplicate. Freshly prepared use solutions were contaminated to determine survival of isolates. Results 66 dispensers containing disinfectant solutions with surface-active ingredients were collected in 15 healthcare facilities. 28 dispensers from nine healthcare facilities were contaminated with approximately 107 cells per mL of Achromobacter species 3 (9 hospitals), Achromobacter xylosoxidans or Serratia marcescens (1 hospital each). In none of the hospitals dispenser processing had been adequately performed. Isolates regained susceptibility to the disinfectants after five passages without selection pressure but were still able to multiply in different formulations from different manufacturers at room temperature within 7 days. Conclusions Neglecting adequate processing of surface disinfectant dispensers has contributed to frequent and heavy contamination of use-solutions based on surface active ingredients. Tissue dispenser processing should be taken seriously in clinical practice. [ABSTRACT FROM AUTHOR]
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- 2014
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11. Effective reprocessing of reusable dispensers for surface disinfection tissues -- the devil is in the details.
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Kampf, Günter, Degenhardt, Stina, Lackner, Sibylle, and Ostermeyer, Christiane
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STERILIZATION equipment , *STERILIZATION (Disinfection) , *BACTERIA , *MICROBIAL contamination , *WASTE recycling , *IN vitro studies - Abstract
Background: It has recently been reported that reusable dispensers for surface disinfection tissues may be contaminated, especially with adapted Achromobacter species 3, when products based on surface-active ingredients are used. Fresh solution may quickly become recontaminated if dispensers are not processed adequately. Methods: We evaluated the abilities of six manual and three automatic processes for processing contaminated dispensers to prevent recolonisation of a freshly-prepared disinfectant solution (Mikrobac forte 0.5%). Dispensers were left at room temperature for 28 days. Samples of the disinfectant solution were taken every 7 days and assessed quantitatively for bacterial contamination. Results: All automatic procedures prevented recolonisation of the disinfectant solution when a temperature of 60-70°C was ensured for at least 5 min, with or without the addition of chemical cleaning agents. Manual procedures prevented recontamination of the disinfectant solution when rinsing with hot water or a thorough cleaning step was performed before treating all surfaces with an alcohol-based disinfectant or an oxygen-releaser. Other cleaning and disinfection procedures, including the use of an alcohol-based disinfectant, did not prevent recolonisation. Conclusions: These results indicate that not all processes are effective for processing reusable dispensers for surface-disinfectant tissues, and that a high temperature during the cleaning step or use of a biofilm-active cleaning agent are essential. [ABSTRACT FROM AUTHOR]
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- 2014
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12. Effect of temperature on microcystin-LR removal and lysis activity on Microcystis aeruginosa (cyanobacteria) by an indigenous bacterium belonging to the genus Achromobacter
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Ivan Juarez, Cristina S. Costa, Leda Giannuzzi, Darío Andrinolo, Melina Celeste Crettaz-Minaglia, Maximiliano Fallico, Oswaldo Aranda, and Magdalena Pezzoni
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Cyanobacteria ,2019-20 coronavirus outbreak ,Lysis ,BIOLOGICAL REMOVAL ,Health, Toxicology and Mutagenesis ,RIO DE LA PLATA RIVER ,Microcystin-LR ,010501 environmental sciences ,01 natural sciences ,Microbiology ,Ciencias Biológicas ,chemistry.chemical_compound ,Biología Celular, Microbiología ,Genus Achromobacter ,Environmental Chemistry ,Ecotoxicology ,Biological removal ,Rio de la Plata River ,Microcystis aeruginosa ,Ciencias Exactas ,0105 earth and related environmental sciences ,biology ,ACHROMOBACTER SPP ,Microcystislysis ,General Medicine ,Química ,biology.organism_classification ,Pollution ,chemistry ,MICROCYSTIS LYSIS ,Achromobacter spp ,Bacteria ,CIENCIAS NATURALES Y EXACTAS - Abstract
Microcystis is a frequent cyanobacterium bloom-forming with cosmopolitan distribution which can produce a hepatotoxin group called microcystins (MCs). These MCs are resistant to the traditional processes employed in the water treatment plants and they are often detected after conventional treatments. Because of this, the bio-removal studies have obtained a great interest in the last decades. In this work, a bacterial strain namely LG1 with the ability to remove microcystin-LR (MC-LR) under laboratory conditions was isolated from Rio de la Plata River and it was identified as Achromobacter spp. This ubiquitous bacterium was able to remove 79.5% MC-LR in 7 days with average removal time of 3.33 ± 0.08, 3.06 ± 0.05, and 2.77 ± 0.05 days at 28, 32, and 36 ± 1 °C, being higher at high temperature (36 °C) with an activation energy = 16.79 ± 1.99 kJ mol⁻¹. LG1 grew better at higher temperature (from 28 to 36 ± 1 °C) increasing the specific growth rate (μ) and reducing 2-fold the lag phase duration (LPD) without significant differences (p > 0.05) between maximum population density (MPD). In addition, LG1 showed a lysis activity on two M. aeruginosa native strains in 7 days measured as chlorophyll a (Chl-a) concentration. The lysis activity increased around 2-fold when increasing the temperature from 28 to 36 ± 1 °C. This is the first report of an indigenous bacterium belonging to the genusAchromobacter spp. isolated from the Rio de la Plata River with the capacity to remove MC-LR and lysis activity on M. aeruginosa., Facultad de Ciencias Exactas, Centro de Investigación y Desarrollo en Criotecnología de Alimentos
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- 2020
13. P139 Hypermutation as an evolutionary mechanism for Achromobacter spp. in cystic fibrosis lung infection
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Giovanni Malerba, Angela Sandri, Laura Veschetti, M. M. Lleò, R. Passarelli Mantovani, and P. Melotti
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Pulmonary and Respiratory Medicine ,Achromobacter ,biology ,Mechanism (biology) ,business.industry ,Lung infection ,Hypermutation ,Somatic hypermutation ,medicine.disease ,biology.organism_classification ,Cystic fibrosis ,cystic fibrosis lung infection ,evolutionary mechanism ,Achromobacter spp ,Pediatrics, Perinatology and Child Health ,Immunology ,medicine ,Hypermutation, evolutionary mechanism, Achromobacter spp, cystic fibrosis lung infection ,business - Published
- 2020
14. In vitro susceptibility of Achromobacter spp. isolates: comparison of disk diffusion, Etest and agar dilution methods
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Almuzara, Marisa, Limansky, Adriana, Ballerini, Viviana, Galanternik, Laura, Famiglietti, Angela, and Vay, Carlos
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ANTI-infective agents , *MICROBIAL sensitivity tests , *BACTERIAL disease treatment , *DRUG resistance in microorganisms , *ANTIBIOTICS , *PIPERACILLIN , *LEAST squares , *TETRACYCLINES - Abstract
Abstract: In this study, we analysed the antimicrobial susceptibility of 92 strains of Achromobacter spp. isolated from clinical samples to 18 antimicrobial agents. The disk diffusion method and Etest were compared with the agar dilution method, and the breakpoints of susceptibility and resistance for the disk diffusion method for the antimicrobials tested were determined. The most active antibiotics were piperacillin, piperacillin/tazobactam and the carbapenems. By applying the linear least-squares regression method, breakpoints could be established for antibiotics active against this genus such as imipenem, meropenem, ertapenem and trimethoprim/sulfamethoxazole (SXT). Other active antibiotics, such as piperacillin and minocycline, could be tested by the Etest method. The less active antibiotics such as gentamicin, doxycycline and tetracycline could be tested by the disk diffusion method. For the rest of the antimicrobial agents tested, breakpoints could not be established owing to the high percentage of errors and/or the poor linear regression coefficient obtained. Therefore, these antimicrobial agents should be tested by minimal inhibitory concentration determination. In summary, we recommend the following zone diameter breakpoints for resistant and susceptible, respectively: ≤11mm and ≥22mm for imipenem; ≤13mm and ≥24mm for meropenem; ≤17mm and ≥24mm for ertapenem; ≤15mm and ≥21mm for gentamicin; ≤27mm and ≥28mm for SXT; ≤20mm and ≥29mm for tetracycline; and ≤20mm and ≥24mm for doxycycline. [Copyright &y& Elsevier]
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- 2010
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15. Achromobacter xylosoxidans infection in cystic fibrosis siblings with different outcomes: Case reports
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Rosana Helena Vicente Pereira, Elizabeth Andrade Marques, Pedro Daltro, Domenico Capone, Tania Wrobel Folescu, Rodolpho Mattos Albano, Robson Souza Leão, Agnaldo José Lopes, Vagner Gonçalves Bernardo, Elenice R. A. Rodrigues, and Mônica de Cássia Firmida
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,Achromobacter xylosoxidans ,Achromobacter ,Swarming motility ,Case Report ,medicine.disease_cause ,Cystic fibrosis ,Pulmonary function testing ,Microbiology ,03 medical and health sciences ,Genotype ,Medicine ,Clinical significance ,lcsh:RC705-779 ,biology ,business.industry ,Pseudomonas aeruginosa ,lcsh:Diseases of the respiratory system ,biology.organism_classification ,medicine.disease ,030104 developmental biology ,Immunology ,Achromobacter spp ,business - Abstract
Introduction The clinical relevance of Achromobacter xylosoxidans infection in cystic fibrosis (CF) remains controversial. This emerging agent in CF has been associated with increased lung inflammation, more frequent exacerbations and more severe lung disease. We describe a pair of CF siblings chronically colonized by the same multilocus genotype of A. xylosoxidans with different clinical courses, and assess whether this species may have developed any virulence traits and antimicrobial resistance that could have contributed to their singular outcomes. Case presentation Two siblings were positive for the F508del and Y1092X mutations, and were chronically colonized by Pseudomonas aeruginosa and Staphylococcus aureus. The female patient had a more severe CF phenotype and faster clinical deterioration than her brother. Her pulmonary function and computed tomography scan lesions were worse than those of her brother, and both parameters progressively declined. She died at 14 years of age, when he was 18. All isolates of A. xylosoxidans were biofilm producers. Achromobacter xylosoxidans showed less swarming motility in the female patient. Conclusions Biofilm production and diminution of motility allow persistence. Only swarming motility differed between the isolates recovered from the two siblings, but this finding is not sufficient to explain the different clinical outcomes despite their similar genotypes. Modifier genes, unknown environmental factors and female gender can partially explain differences between these siblings. We were unable to correlate any microbiological findings with their clinical courses, and more translational studies are necessary to decrease the gap of knowledge between laboratory and clinical data to promote better clinical interventions.
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- 2017
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16. Achromobacter xylosoxidans Infections after Prostate Biopsies, France, 2014
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Catherine Neuwirth, Julien Bador, and Lucie Amoureux
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Male ,Microbiology (medical) ,reservoir ,Letter ,Epidemiology ,Biopsy ,species identification ,lcsh:Medicine ,Disease Outbreaks ,Microbiology ,lcsh:Infectious and parasitic diseases ,resistance ,Prostate ,medicine ,nosocomial infections ,Humans ,Species identification ,lcsh:RC109-216 ,Letters to the Editor ,bacteria ,Cross Infection ,biology ,lcsh:R ,Achromobacter xylosoxidans ,biology.organism_classification ,Infectious Diseases ,medicine.anatomical_structure ,Achromobacter xylosoxidans Infections after Prostate Biopsies, France, 2014 ,Achromobacter denitrificans ,France ,Gram-Negative Bacterial Infections ,Achromobacter spp ,Bacteria - Published
- 2019
17. Characterization of a Sequential UV Photolysis-Biodegradation Process for Treatment of Decabrominated Diphenyl Ethers in Sorbent/Water Systems
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Stephen Boyd, Hsin-Yu Chen, Hui Li, Wei-Liang Chao, and Yi-Tang Chang
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0301 basic medicine ,Microbiology (medical) ,decabrominated diphenyl ether ,Sorbent ,Novosphingobium ,PBDE congeners ,sequential photolysis- microbial biodegradation ,030106 microbiology ,Achromobacter spp ,010501 environmental sciences ,01 natural sciences ,Microbiology ,Article ,emerging contaminant ,03 medical and health sciences ,chemistry.chemical_compound ,Virology ,Bioreactor ,lcsh:QH301-705.5 ,0105 earth and related environmental sciences ,Aqueous solution ,biology ,Chemistry ,Diphenyl ether ,Biodegradation ,Sphingomonas ,biology.organism_classification ,lcsh:Biology (General) ,Environmental chemistry ,Slurry - Abstract
Decabrominated diphenyl ether (BDE-209) is a primary component of the brominated flame retardants used in a variety of industrial and domestic applications. BDE-209 bioaccumulates in aquatic organisms and has been identified as an emerging contaminant that threatens human and ecosystem health. Sequential photolysis-microbial biodegradation processes were utilized here to treat BDE-209 in clay- or soil-water slurries. The removal efficiency of BDE-209 in the clay-water slurries was high, i.e., 96.5%, while that in the soil-water slurries was minimal. In the clay-water slurries the first order rate constants for the UV photolysis and biodegradation of BDE-209 were 0.017 1/day and 0.026 1/day, respectively. UV wavelength and intensity strongly influenced the BDE-209 photolysis and the subsequent biodegradation of photolytic products. Facultative chemotrophic bacteria, including Acidovorax spp., Pseudomonas spp., Novosphingobium spp. and Sphingomonas spp., were the dominant members of the bacterial community (about 71%) at the beginning of the biodegradation, many of these organisms have previously been shown to biodegrade BDE-209 and other polybrominated diphenyl ether (PBDE) congeners. The Achromobacter sp. that were isolated (NH-2, NH-4, NH-6) were especially effective during the BDE-209 degradation. These results indicated the effectiveness of the sequential UV photolysis and biodegradation for treating certain BDE-209-contaminated solids, e.g., clays, in bioreactors containing such solids as aqueous slurries. Achieving a similar treatment effectiveness for more heterogeneous solids containing natural organic matter, e.g., surface solids, appears to be significantly more difficult. Further investigations are needed in order to understand the great difference between the clay-water or soil-water slurries.
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- 2020
18. Characterisation of OXA-258 enzymes and AxyABM efflux pump in Achromobacter ruhlandii
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Marcela Alejandra Radice, Carlos Vay, Maria Soledad Ramirez, Marisa Almuzara, German Matias Traglia, Mariana Andrea Papalia, Melina Ruggiero, and Gabriel Osvaldo Gutkind
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0301 basic medicine ,Microbiology (medical) ,ACHROMOBACTER SPP ,030106 microbiology ,Immunology ,Achromobacter ,Microbial Sensitivity Tests ,Achromobacter ruhlandii ,Microbiology ,beta-Lactam Resistance ,beta-Lactamases ,purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,03 medical and health sciences ,Nalidixic Acid ,Bacterial Proteins ,Biología Celular, Microbiología ,Trimethoprim, Sulfamethoxazole Drug Combination ,EFFLUX PUMP ,Immunology and Allergy ,Computer Simulation ,purl.org/becyt/ford/1.6 [https] ,chemistry.chemical_classification ,ANTIMICROBIAL RESISTANCE ,biology ,Whole Genome Sequencing ,Hydrolysis ,OXA-258 ,Genetic Variation ,biology.organism_classification ,Anti-Bacterial Agents ,Imipenem ,Enzyme ,Chloramphenicol ,chemistry ,ACHROMOBACTER RUHLANDII ,Efflux ,CIENCIAS NATURALES Y EXACTAS ,Bacterial Outer Membrane Proteins - Abstract
Objectives: The aim of this study was to characterise OXA-258 variants and other features that may contribute to carbapenem resistance in Achromobacter ruhlandii. Methods: Kinetic parameters for purified OXA-258a and OXA-258b were determined measuring the rate of hydrolysis of a representative group of antimicrobial agents. Whole-genome shotgun sequencing was performed on A. ruhlandii 38 (producing OXA-258a) and A. ruhlandii 319 (producing OXA-258b), and in silico analysis of antimicrobial resistance determinants was conducted. Substrates of the AxyABM efflux pump were investigated by inhibition assays using phenylalanine-arginine β-naphthylamide (PAβN). Outer membrane protein profiles were resolved by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results: Kinetic measurements of purified OXA-258 variants displayed an overall weak catalytic efficiency toward β-lactams. A detectable hydrolysis of imipenem was observed. In silico genomic analysis confirmed the presence of 32 and 35 putative efflux pump-encoding genes in A. ruhlandii strains 38 and 319, respectively. Complete sequences for AxyABM and AxyXY efflux pumps, previously described in Achromobacter xylosoxidans, were detected. Decreases in the MICs for chloramphenicol, nalidixic acid and trimethoprim/sulfamethoxazole were observed in the presence of the inhibitor PAβN, suggesting that these antibiotics are substrates of AxyABM. AxyXY-encoding genes of A. ruhlandii 38 and A. ruhlandii 319 displayed 99% identity. No differences were observed in the outer membrane protein profiles. Conclusions: The contribution of OXA-258 enzymes to the final β-lactam resistance profile may be secondary. Further studies on other putative resistance markers identified in the whole-genome analysis should be conducted to understand the carbapenem resistance observed in A. ruhlandii. Fil: Papalia, Mariana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Ruggiero, Melina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Almuzara, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Ramírez, María Soledad. California State University; Estados Unidos Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
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- 2018
19. On Burkholderiales order microorganisms and cystic fibrosis in Russia
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Ekaterina I. Aksenova, Alexandr L. Gintsburg, M.S. Kunda, Natalia N. Ryzhova, Alexandr G. Chuchalin, Natalia E. Sharapova, Andrey N. Semenov, Elena L. Amelina, and Olga L. Voronina
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Adult ,Male ,0301 basic medicine ,Massively parallel sequencing ,Achromobacter ,lcsh:QH426-470 ,Adolescent ,Cystic Fibrosis ,Epidemic strain ,lcsh:Biotechnology ,030106 microbiology ,Lautropia mirabilis ,Cystic fibrosis ,Russia ,Microbiology ,Young Adult ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Genotype ,Genetics ,medicine ,Humans ,Microbiome ,Child ,Burkholderiales ,Aged ,biology ,Microbiota ,Research ,Burkholderia cepacia complex ,Genetic Variation ,Infant ,Middle Aged ,biology.organism_classification ,medicine.disease ,lcsh:Genetics ,030104 developmental biology ,Child, Preschool ,Whole genome sequencing ,Sputum ,Female ,medicine.symptom ,Achromobacter spp ,Biotechnology - Abstract
Background Microbes infecting cystic fibrosis patients’ respiratory tract are important in determining patients’ functional status. Representatives of Burkholderiales order are the most dangerous. The goal of our investigation was to reveal the diversity of Burkholderiales, define of their proportion in the microbiome of various parts of respiratory tract and determine the pathogenicity of the main representatives. Results In more than 500 cystic fibrosis patients, representing all Federal Regions of Russia, 34.0% were infected by Burkholderia cepacia complex (Bcc), 21.0% by Achromobacter spp. and 12.0% by Lautropia mirabilis. B. cenocepacia was the most numerous species among the Bcc (93.0%), and A. ruhlandii was the most numerous among Achromobacter spp. (58.0%). The most abundant genotype in Bcc was sequence type (ST) 709, and in Achromobacter spp. it was ST36. These STs constitute Russian epidemic strains. Whole genome sequencing of strains A. ruhlandii SCCH3:Ach33–1365 ST36 and B. cenocepacia GIMC4560:Bcn122 ST709 revealed huge resistomes and many virulence factors, which may explain the difficulties in eradicating these strains. An experience of less dangerous B. cenocepcia ST710 elimination was described. Massively parallel sequencing of 16S rDNA amplicons, including V1-V4 hypervariable regions, was used to definite “healthy” microbiome characteristics. Analysis of maxillary sinus lavage of 7 patients revealed infection with Proteobacteria of the same ST as pathogens from sputum, suggesting that the maxillary sinus is a source of infection in cystic fibrosis patients. Conclusions Characterization of the Russian epidemic bacterial strains in the sputum and sinuses of cystic fibrosis patients have better defined the importance of Burkholderiales bacteria. This information may aid in the development of effective approaches for treatment of this disease. Electronic supplementary material The online version of this article (10.1186/s12864-018-4472-9) contains supplementary material, which is available to authorized users.
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- 2018
20. Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
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Brankica Filipic, Milka Malesevic, Zorica Vasiljevic, Jovanka Lukic, Katarina Novovic, Milan Kojic, and Branko Jovcic
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0301 basic medicine ,virulence traits ,Male ,Cystic Fibrosis ,lcsh:QR1-502 ,Achromobacter ,lcsh:Microbiology ,RNA, Ribosomal, 16S ,Genotype ,Child ,Phylogeny ,Original Research ,Molecular Epidemiology ,biology ,Virulence ,Antimicrobial ,3. Good health ,nrdA ,Anti-Bacterial Agents ,Infectious Diseases ,Phenotype ,Child, Preschool ,Multigene Family ,Female ,Achromobacter spp ,Locomotion ,Microbiology (medical) ,Adult ,Adolescent ,030106 microbiology ,Immunology ,Microbial Sensitivity Tests ,Microbiology ,03 medical and health sciences ,Young Adult ,Antibiotic resistance ,Drug Resistance, Bacterial ,Humans ,antimicrobial resistance ,Adhesins, Bacterial ,Molecular epidemiology ,Mucin ,Biofilm ,Infant, Newborn ,Infant ,biology.organism_classification ,030104 developmental biology ,Genes, Bacterial ,Biofilms ,Gram-Negative Bacterial Infections - Abstract
Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.
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- 2017
21. Clinical impact of Achromobacter xylosoxidans colonization/infection in patients with cystic fibrosis
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Agnaldo José Lopes, E.A.S.R. Silva, Rosana Helena Vicente Pereira, Elizabeth Andrade Marques, and Mônica de Cássia Firmida
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Male ,0301 basic medicine ,Pathology ,Time Factors ,Cystic Fibrosis ,Physiology ,medicine.disease_cause ,Biochemistry ,Cystic fibrosis ,Gastroenterology ,0302 clinical medicine ,Reference Values ,Forced Expiratory Volume ,Colonization ,General Pharmacology, Toxicology and Pharmaceutics ,Child ,lcsh:QH301-705.5 ,Lung ,lcsh:R5-920 ,biology ,General Neuroscience ,Age Factors ,Achromobacter denitrificans ,General Medicine ,Achromobacter xylosoxidans ,medicine.anatomical_structure ,Child, Preschool ,Population study ,Female ,lcsh:Medicine (General) ,Achromobacter spp ,Adult ,Methicillin-Resistant Staphylococcus aureus ,medicine.medical_specialty ,Adolescent ,030106 microbiology ,Immunology ,Biophysics ,Microbiology ,Statistics, Nonparametric ,Young Adult ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,Clinical significance ,Clinical Investigation ,Retrospective Studies ,Analysis of Variance ,business.industry ,Infant ,Cell Biology ,biology.organism_classification ,medicine.disease ,Methicillin-resistant Staphylococcus aureus ,digestive system diseases ,lcsh:Biology (General) ,030228 respiratory system ,Case-Control Studies ,Gram-Negative Bacterial Infections ,business - Abstract
The rate of diagnosis of colonization/infection of the airways with Achromobacter xylosoxidans has increased in cystic fibrosis patients, but its clinical significance is still controversial. This retrospective, case-control study aimed to evaluate the clinical impact of A. xylosoxidans colonization/infection in cystic fibrosis patients. Individuals who were chronically colonized/infected (n=10), intermittently colonized/infected (n=15), and never colonized/infected with A. xylosoxidans (n=18) were retrospectively evaluated during two periods that were 2 years apart. Demographic characteristics, clinical data, lung function, and chronic bacterial co-colonization data were evaluated. Of the total study population, 87% were pediatric patients and 65.1% were female. Individuals chronically colonized/infected with A. xylosoxidans had decreased forced expiratory volume in 1 s (51.7% in the chronic colonization/infection group vs 82.7% in the intermittent colonization/infection group vs 76% in the never colonized/infected group). Compared with the other two groups, the rate of co-colonization with methicillin-resistant Staphylococcus aureus was higher in individuals chronically colonized/infected with A. xylosoxidans (P=0.002). Changes in lung function over 2 years in the three groups were not significant, although a trend toward a greater decrease in lung function was observed in the chronically colonized/infected group. Compared with the other two groups, there was a greater number of annual hospitalizations in patients chronically colonized/infected with A. xylosoxidans (P=0.033). In cystic fibrosis patients, there was an increased frequency of A. xylosoxidans colonization/infection in children, and lung function was reduced in patients who were chronically colonized/infected with A. xylosoxidans. Additionally, there were no differences in clinical outcomes during the 2-year period, except for an increased number of hospitalizations in patients with A. xylosoxidans.
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- 2016
22. Wirksame Aufbereitung von wiederverwendbaren Spendern für Flächendesinfektionstücher - der Teufel steckt im Detail
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Kampf, Günter, Degenhardt, Stina, Lackner, Sibylle, and Ostermeyer, Christiane
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lcsh:Public aspects of medicine ,lcsh:R ,lcsh:QR1-502 ,lcsh:Medicine ,lcsh:RA1-1270 ,tissue dispenser ,610 Medical sciences ,Medicine ,Tuchspender ,lcsh:Microbiology ,Article ,Flächendesinfektion ,ddc: 610 ,surface disinfection ,processing ,Aufbereitung ,Achromobacter spp - Abstract
Background: It has recently been reported that reusable dispensers for surface disinfection tissues may be contaminated, especially with adapted Achromobacter species 3, when products based on surface-active ingredients are used. Fresh solution may quickly become recontaminated if dispensers are not processed adequately. Methods: We evaluated the abilities of six manual and three automatic processes for processing contaminated dispensers to prevent recolonisation of a freshly-prepared disinfectant solution (Mikrobac forte 0.5%). Dispensers were left at room temperature for 28 days. Samples of the disinfectant solution were taken every 7 days and assessed quantitatively for bacterial contamination. Results: All automatic procedures prevented recolonisation of the disinfectant solution when a temperature of 60–70°C was ensured for at least 5 min, with or without the addition of chemical cleaning agents. Manual procedures prevented recontamination of the disinfectant solution when rinsing with hot water or a thorough cleaning step was performed before treating all surfaces with an alcohol-based disinfectant or an oxygen-releaser. Other cleaning and disinfection procedures, including the use of an alcohol-based disinfectant, did not prevent recolonisation. Conclusions: These results indicate that not all processes are effective for processing reusable dispensers for surface-disinfectant tissues, and that a high temperature during the cleaning step or use of a biofilm-active cleaning agent are essential., GMS Hygiene and Infection Control; 9(1):Doc09; ISSN 2196-5226
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- 2014
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23. Trends in ceftazidime‐avibactam activity against multidrug‐resistant organisms recovered from respiratory samples of cystic fibrosis patients.
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Farfour, Eric, Trochu, Erwan, Devin, Clotilde, Cardot Martin, Emilie, Limousin, Lucie, Jolly, Emilie, Vasse, Marc, Lesprit, Philippe, Roux, Antoine, and Picard, Clément
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STENOTROPHOMONAS maltophilia , *ACHROMOBACTER , *BURKHOLDERIA cepacia , *CEFTAZIDIME , *DISC diffusion tests (Microbiology) , *CYSTIC fibrosis , *PATIENTS - Abstract
The article discusses a study to examine in vitro susceptibility of Stenotrophomonas maltophilia, Achromobacter, and Burkholderia cepacia complex (Bcc) isolates to ceftazidime-avibactam and to other antibiotics by the disc diffusion method. Topics discussed include colonization of multidrug-resistant micro-organisms in cystic fibrosis (CF) patient, testing of minocycline against Bcc, and activity of ceftazidime-avibactam against Bcc.
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- 2018
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24. Poorly processed reusable surface disinfection tissue dispensers may be a source of infection
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Sibylle Lackner, Stina Degenhardt, Heike von Baum, Christiane Ostermeyer, Katrin Jesse, and Günter Kampf
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Achromobacter species ,biology ,Disinfectant ,Biofilm ,Achromobacter xylosoxidans ,Human decontamination ,Achromobacter ,Contamination ,biology.organism_classification ,Microbiology ,Reusable dispenser ,Clinical Practice ,Toxicology ,Disinfection ,Surface-active biocidal ingredients ,Infectious Diseases ,Achromobacter spp ,Surface disinfection ,Adaptation ,Drug Contamination ,Disease transmission ,Serratia marcescens ,Bacterial contamination ,Disinfectants ,Research Article - Abstract
Background Reusable surface disinfectant tissue dispensers are used in hospitals in many countries because they allow immediate access to pre-soaked tissues for targeted surface decontamination. On the other hand disinfectant solutions with some active ingredients may get contaminated and cause outbreaks. We determined the frequency of contaminated surface disinfectant solutions in reusable dispensers and the ability of isolates to multiply in different formulations. Methods Reusable tissue dispensers with different surface disinfectants were randomly collected from healthcare facilities. Solutions were investigated for bacterial contamination. The efficacy of two surface disinfectants was determined in suspension tests against two isolated species directly from a contaminated solution or after 5 passages without selection pressure in triplicate. Freshly prepared use solutions were contaminated to determine survival of isolates. Results 66 dispensers containing disinfectant solutions with surface-active ingredients were collected in 15 healthcare facilities. 28 dispensers from nine healthcare facilities were contaminated with approximately 107 cells per mL of Achromobacter species 3 (9 hospitals), Achromobacter xylosoxidans or Serratia marcescens (1 hospital each). In none of the hospitals dispenser processing had been adequately performed. Isolates regained susceptibility to the disinfectants after five passages without selection pressure but were still able to multiply in different formulations from different manufacturers at room temperature within 7 days. Conclusions Neglecting adequate processing of surface disinfectant dispensers has contributed to frequent and heavy contamination of use-solutions based on surface active ingredients. Tissue dispenser processing should be taken seriously in clinical practice.
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