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Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
- Source :
- Frontiers in Cellular and Infection Microbiology, Frontiers in Cellular and Infection Microbiology, Vol 7 (2017)
- Publication Year :
- 2017
- Publisher :
- Frontiers Media S.A., 2017.
-
Abstract
- Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.
- Subjects :
- 0301 basic medicine
virulence traits
Male
Cystic Fibrosis
lcsh:QR1-502
Achromobacter
lcsh:Microbiology
RNA, Ribosomal, 16S
Genotype
Child
Phylogeny
Original Research
Molecular Epidemiology
biology
Virulence
Antimicrobial
3. Good health
nrdA
Anti-Bacterial Agents
Infectious Diseases
Phenotype
Child, Preschool
Multigene Family
Female
Achromobacter spp
Locomotion
Microbiology (medical)
Adult
Adolescent
030106 microbiology
Immunology
Microbial Sensitivity Tests
Microbiology
03 medical and health sciences
Young Adult
Antibiotic resistance
Drug Resistance, Bacterial
Humans
antimicrobial resistance
Adhesins, Bacterial
Molecular epidemiology
Mucin
Biofilm
Infant, Newborn
Infant
biology.organism_classification
030104 developmental biology
Genes, Bacterial
Biofilms
Gram-Negative Bacterial Infections
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular and Infection Microbiology
- Accession number :
- edsair.doi.dedup.....f8624d502bb735f60c79768c5ea5d9b4