1. Parasitic modulation of host development by ubiquitin-independent protein degradation
- Author
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Sophien Kamoun, Chih-Horng Kuo, Richard G. H. Immink, Shu-Ting Cho, Saskia A. Hogenhout, Akiko Sugio, Marco Busscher, Weijie Huang, Abbas Maqbool, Allyson M. MacLean, John Innes Centre [Norwich], Biotechnology and Biological Sciences Research Council (BBSRC), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of East Anglia [Norwich] (UEA), Wageningen University and Research [Wageningen] (WUR), Academica Sinica, Academia Sinica, The project was supported by Human Frontier Science Program RGP0024/2015 (to S.A.H. and R.G.H.I.) and Marie Curie International Incoming Fellowship FP7-PEOPLE-2010-IIF grant agreement 274444 (to A.M.M.). Additional support was received from Biotechnology and Biological Sciences Research Council grants BBS/E/J/000PR9797 (to S.A.H.), the John Innes Foundation (to S.A.H.), and Academia Sinica intramural funding (to C.K.)., and European Project: 274444,EC:FP7:PEOPLE,FP7-PEOPLE-2010-IIF,AY-WB EFFECTORS(2011)
- Subjects
Insecta ,Transcription, Genetic ,Arabidopsis ,phloem ,Ubiquitin ,developmental phase transition ,Phylogeny ,Protein Stability ,Effector ,Reproduction ,Plants, Genetically Modified ,Phenotype ,plant pathogen ,Cell biology ,Laboratory of Molecular Biology ,Genetic Engineering ,Plant Shoots ,zombie plant ,Proteasome Endopeptidase Complex ,vasculature-colonizing bacteria ,Phytoplasma ,Photoperiod ,Plant Development ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Protein degradation ,Biology ,Models, Biological ,non-culturable bacteria ,General Biochemistry, Genetics and Molecular Biology ,Host-Parasite Interactions ,Tobacco ,Animals ,Humans ,Laboratorium voor Moleculaire Biologie ,Parasites ,Amino Acid Sequence ,BIOS Plant Development Systems ,mycoplasma ,Ubiquitins ,Obligate ,Arabidopsis Proteins ,Host (biology) ,fungi ,insect vectors ,Obligate parasite ,Proteasome ,Proteolysis ,biology.protein ,ubiquitin-proteasome system ,targeted protein degradation ,Transcription Factors ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis - Abstract
International audience; Certain obligate parasites induce complex and substantial phenotypic changes in their hosts in ways that favor their transmission to other trophic levels. However, the mechanisms underlying these changes remain largely unknown. Here we demonstrate how SAP05 protein effectors from insect-vectored plant pathogenic phytoplasmas take control of several plant developmental processes. These effectors simultaneously prolong the host lifespan and induce witches' broom-like proliferations of leaf and sterile shoots, organs colonized by phytoplasmas and vectors, SAP05 acts by mediating the concurrent degradation of SPL and GATA developmental regulators via a process that relies on hijacking the plant ubiquitin receptor RPN10 independent of substrate ubiquitination. RPN10 is highly conserved among eukaryotes, but SAP05 does not bind insect vector RPN10. A two-amino-acid substitution within plant RPN10 generates a functional variant that is resistant to SAP05 activities. Therefore, one effector protein enables obligate parasitic phytoplasmas to induce a plethora of developmental phenotypes in their hosts.
- Published
- 2021