1. New World Cactaceae Plants Harbor Diverse Geminiviruses
- Author
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Gerardo R. Argüello-Astorga, Arvind Varsani, Michael C. Lund, Simona Kraberger, Pierre Lefeuvre, Joshua Schreck, Denis Filloux, Anthony Khalifeh, Simone G. Ribeiro, Raul Puente-Martinez, Jesús Aarón Avalos-Calleros, Andrew M. Salywon, Safaa G. Kumari, Philippe Roumagnac, Kehinde A. Oyeniran, Kara Schmidlin, Rafaela S. Fontenele, Lucas C. Majure, Amulya Bhaskara, Wendy C. Hodgson, Martin F. Wojciechowski, Matias Köhler, Darren P. Martin, Kendal Smith, Koenraad Van Doorslaer, Christian Vernière, Ilaria N. Cobb, Arizona State University [Tempe] (ASU), The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Desert Botanical Garden, Florida Museum of Natural History [Gainesville], University of Florida [Gainesville] (UF), University of British Columbia [Vancouver], University of Texas at Dallas [Richardson] (UT Dallas), School of Behavioral and Brain Sciences, Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Laboratório de Estudos em Vegetação Campestre (LEVCamp), Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil, School of Animal and Comparative Biomedical Sciences, University of Arizona, Arizona Cancer Center, International Center for Agricultural Research in the Dry Areas (ICARDA), Consultative Group on International Agricultural Research [CGIAR] (CGIAR), University of Cape Town, Institute of Infectious Diseases and Moleculare MedicineComputational Biolohy Group, Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Embrapa Recursos Genéticos e Biotecnologia [Brasília], Structural Biology Research Unit, Department of Clinical Laboratory Sciences, The molecular work was supported by a start-up grant awarded to A.V. from Arizona State University, USA., RAFAELA S. FONTENELE, ARIZONA STATE UNIVERSITY, USA, ANDREW M. SALYWON, DESERT BOTANICAL GARDEN, USA, LUCAS C. MAJURE, UNIVERSITY OF FLORIDA, USA, ILARIA N. COBB, ARIZONA STATE UNIVERSITY, USA, AMULYA BHASKARA, ARIZONA STATE UNIVERSITY, USA, JESÚS A. AVALOS-CALLEROS, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, GERARDO R. ARGÜELLO-ASTORGA, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, KARA SCHMIDLIN, ARIZONA STATE UNIVERSITY, USA, ANTHONY KHALIFEH, ARIZONA STATE UNIVERSITY, USA, KENDAL SMITH, ARIZONA STATE UNIVERSITY, USA, JOSHUA SCHRECK, ARIZONA STATE UNIVERSITY, USA, MICHAEL C. LUND, ARIZONA STATE UNIVERSITY, USA, MATIAS KÖHLER, UFRGS, MARTIN F. WOJCIECHOWSKI, ARIZONA STATE UNIVERSITY, USA, WENDY C. HODGSON, DESERT BOTANICAL GARDEN, USA, RAUL PUENTE-MARTINEZ, DESERT BOTANICAL GARDEN, USA, KOENRAAD VAN DOORSLAER, UNIVERSITY OF ARIZONA, USA, SAFAA KUMARI, INTERNATIONAL CENTER FOR AGRICULTURAL RESEARCH IN THE DRY AREAS (ICARDA), LEBANON, KEHINDE A. OYENIRAN, UNIVERSITY OF CAPE TOWN, SOUTH AFRICA, CHRISTIAN VERNIÈRE, CIRAD, FRANCE, DENIS FILLOUX, CIRAD, FRANCE, PHILIPPE ROUMAGNAC, CIRAD, FRANCE, PIERRE LEFEUVRE, CIRAD, FRANCE, SIMONE DA GRACA RIBEIRO, Cenargen, SIMONA P. KRABERGER, ARIZONA STATE UNIVERSITY, USA, DARREN P. MARTIN, UNIVERSITY OF CAPE TOWN, SOUTH AFRICA, ARVIND VARSANI, ARIZONA STATE UNIVERSITY, USA., University of Britsh Columbia [Vancouver], Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Human Biology, and Faculty of Health Sciences
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0106 biological sciences ,0301 basic medicine ,Cactaceae ,Phylogénie ,[SDV]Life Sciences [q-bio] ,01 natural sciences ,Genome ,Curtovirus ,2. Zero hunger ,Diversity ,biology ,Phylogenetic tree ,Opuntia ,QR1-502 ,Infectious Diseases ,Geminiviridae ,Curly top ,Cactus ,Becurtovirus ,food.ingredient ,Pathologie végétale ,Genome, Viral ,Microbiology ,Article ,diversity ,Hemiptera ,03 medical and health sciences ,food ,Virology ,Animals ,Virus classification ,Géminivirus ,Plant Diseases ,H20 - Maladies des plantes ,Virus des végétaux ,biology.organism_classification ,Recombination ,recombination ,Recombinaison ,030104 developmental biology ,Evolutionary biology ,010606 plant biology & botany - Abstract
International audience; The family Cactaceae comprises a diverse group of typically succulent plants that are native to the American continent but have been introduced to nearly all other continents, predominantly for ornamental purposes. Despite their economic, cultural, and ecological importance, very little research has been conducted on the viral community that infects them. We previously identified a highly divergent geminivirus that is the first known to infect cacti. Recent research efforts in non-cultivated and asymptomatic plants have shown that the diversity of this viral family has been under-sampled. As a consequence, little is known about the effects and interactions of geminiviruses in many plants, such as cacti. With the objective to expand knowledge on the diversity of geminiviruses infecting cacti, we used previously acquired high-throughput sequencing results to search for viral sequences using BLASTx against a viral RefSeq protein database. We identified two additional sequences with similarity to geminiviruses, for which we designed abutting primers and recovered full-length genomes. From 42 cacti and five scale insects, we derived 42 complete genome sequences of a novel geminivirus species that we have tentatively named Opuntia virus 2 (OpV2) and 32 genomes of an Opuntia-infecting becurtovirus (which is a new strain of the spinach curly top Arizona virus species). Interspecies recombination analysis of the OpV2 group revealed several recombinant regions, in some cases spanning half of the genome. Phylogenetic analysis demonstrated that OpV2 is a novel geminivirus more closely related to viruses of the genus Curtovirus, which was further supported by the detection of three recombination events between curtoviruses and OpV2. Both OpV2 and Opuntia becurtoviruses were identified in mixed infections, which also included the previously characterized Opuntia virus 1. Viral quantification of the co-infected cactus plants compared with single infections did not show any clear trend in viral dynamics that might be associated with the mixed infections. Using experimental Rhizobium-mediated inoculations, we found that the initial accumulation of OpV2 is facilitated by co-infection with OpV1. This study shows that the diversity of geminiviruses that infect cacti is under-sampled and that cacti harbor diverse geminiviruses. The detection of the Opuntia becurtoviruses suggests spill-over events between viruses of cultivated species and native vegetation. The threat this poses to cacti needs to be further investigated.
- Published
- 2021