Back to Search
Start Over
New World Cactaceae Plants Harbor Diverse Geminiviruses
- Source :
- Viruses, Viruses, 2021, 13 (4), pp.694. ⟨10.3390/v13040694⟩, Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Viruses, MDPI, 2021, 13 (4), pp.694. ⟨10.3390/v13040694⟩, Volume 13, Issue 4, Viruses, Vol 13, Iss 694, p 694 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; The family Cactaceae comprises a diverse group of typically succulent plants that are native to the American continent but have been introduced to nearly all other continents, predominantly for ornamental purposes. Despite their economic, cultural, and ecological importance, very little research has been conducted on the viral community that infects them. We previously identified a highly divergent geminivirus that is the first known to infect cacti. Recent research efforts in non-cultivated and asymptomatic plants have shown that the diversity of this viral family has been under-sampled. As a consequence, little is known about the effects and interactions of geminiviruses in many plants, such as cacti. With the objective to expand knowledge on the diversity of geminiviruses infecting cacti, we used previously acquired high-throughput sequencing results to search for viral sequences using BLASTx against a viral RefSeq protein database. We identified two additional sequences with similarity to geminiviruses, for which we designed abutting primers and recovered full-length genomes. From 42 cacti and five scale insects, we derived 42 complete genome sequences of a novel geminivirus species that we have tentatively named Opuntia virus 2 (OpV2) and 32 genomes of an Opuntia-infecting becurtovirus (which is a new strain of the spinach curly top Arizona virus species). Interspecies recombination analysis of the OpV2 group revealed several recombinant regions, in some cases spanning half of the genome. Phylogenetic analysis demonstrated that OpV2 is a novel geminivirus more closely related to viruses of the genus Curtovirus, which was further supported by the detection of three recombination events between curtoviruses and OpV2. Both OpV2 and Opuntia becurtoviruses were identified in mixed infections, which also included the previously characterized Opuntia virus 1. Viral quantification of the co-infected cactus plants compared with single infections did not show any clear trend in viral dynamics that might be associated with the mixed infections. Using experimental Rhizobium-mediated inoculations, we found that the initial accumulation of OpV2 is facilitated by co-infection with OpV1. This study shows that the diversity of geminiviruses that infect cacti is under-sampled and that cacti harbor diverse geminiviruses. The detection of the Opuntia becurtoviruses suggests spill-over events between viruses of cultivated species and native vegetation. The threat this poses to cacti needs to be further investigated.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cactaceae
Phylogénie
[SDV]Life Sciences [q-bio]
01 natural sciences
Genome
Curtovirus
2. Zero hunger
Diversity
biology
Phylogenetic tree
Opuntia
QR1-502
Infectious Diseases
Geminiviridae
Curly top
Cactus
Becurtovirus
food.ingredient
Pathologie végétale
Genome, Viral
Microbiology
Article
diversity
Hemiptera
03 medical and health sciences
food
Virology
Animals
Virus classification
Géminivirus
Plant Diseases
H20 - Maladies des plantes
Virus des végétaux
biology.organism_classification
Recombination
recombination
Recombinaison
030104 developmental biology
Evolutionary biology
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses, Viruses, 2021, 13 (4), pp.694. ⟨10.3390/v13040694⟩, Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Viruses, MDPI, 2021, 13 (4), pp.694. ⟨10.3390/v13040694⟩, Volume 13, Issue 4, Viruses, Vol 13, Iss 694, p 694 (2021)
- Accession number :
- edsair.doi.dedup.....a8b25622ee34963b57bc57dbe65dedff