1. Enzimatics and antibacterial activities of Bacillus spp. from Abalone (Haliotis asinina)as a probiotic candidate.
- Author
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Faroby, Muhammad Azdy, Yuliana, Nita, Hidayati, Ernin, Sarkono, and Faturrahman
- Subjects
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BACILLUS (Bacteria) , *VIBRIO parahaemolyticus , *DIGESTIVE enzymes , *ANTIBACTERIAL agents , *ABALONES , *PROBIOTICS , *AGAR - Abstract
Microbiota in digestion have an important role in breaking down complex compounds in feed into simpler ones for easy digestion. Abalone requires bacterial extracellular enzymes to aid the digestive process by breaking down complex macromolecules. This study aims to determine the ability of Bacillus from abalone to hydrolyze complex macromolecules, antibacterial activities against pathogenic Vibrios and to select Bacillus that has the potential as a probiotic candidate. Test the ability of Bacillus to hydrolyze several complex compounds such as agar, fat,protein, and starch using SWC agar media which has been enriched by the substrate to be hydrolyzed. Hydrolysis test data were obtained from 2 methods, namely by observing the clear zone using cell culture and the clear zone formed from the cell-free supernatant containing the crude extract of the enzyme. The clear zone formed becomes an indicator of Bacillus capable of hydrolyzing complex compounds. The datawere analyzed descriptively through a semi-quantitative potency test. Test the ability of Bacillus isolates in inhibiting the growth of Vibrio sp. carried out by the well-diffusion method. The results showed that Bacillus SL1K1 was the best at hydrolyzing starch and agar with a starch hydrolysis index of 2.25 and a agar hydrolysis index of 4.73. Isolate Bacillus SL2K2 was the best at hydrolyzing lipids with a lipid hydrolysis index of 1.27, isolate Bacillus CA2K6 was the best at hydrolyzing proteins with a proteinhydrolysis index of 3.13. The enzymatic ability of Bacillus based on the clear zone formed showed that Bacillus SL1K1 isolate was the best at hydrolyzing agar-agar and starch. The antibacterial test results of the four isolates of Bacillus against Vibrio bacteria showed that the isolate of Bacillus CAK1 against Vibrio parahaemolyticus had the highest activity with an average inhibition zone of 14.5 mm. Thus, both Bacillus SLK1 and CAK1 isolates have the potential to be used as probiotic candidates. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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