Synthesis and characterization of chitosan-gold nanoparticles (Cs-AuNPs) conjugate as an antibacterial.

AuNPs (gold nanoparticles) are a type of nanomaterial with a high biocompatibility. The goal of this research is to use the Turkevich method to make AuNPs utilizing HAuCl4.3H2O as a precursor and Na3C6H5O7 as a reduction agent. UV-Vis Spectrophotometer (UV-Vis), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), and Transmission Electron Microscopy (TEM) characterization of the resulting AuNPs revealed an excellent band of Surface Plasmon Resonance (SPR) of about 542 nm, high crystallinity, variable shape, and a size range of 20-40 nm. This feature indicates that the AuNPs produced can be used in biomedical applications. Chitosan is an excellent antibacterial biomaterial. Shrimp shells were used to synthesize chitosan nanoparticle (CsNPs) in three stages: deproteination, demineralization, and deacetylation. UV-Vis, FTIR, and XRD techniques were used to characterize the resultant chitosan, and it was discovered to be of good quality. The antibacterial properties of chitosan nanoparticles (CsNPs) and chitosan-AuNPs conjugate (Cs-AuNPs) were investigated using the agar diffusion method. CsNPs and Cs-AuNPs were found to effectively diminish the growth of Escherichia coli bacteria, but not Staphylococcus aureus or Enterococcus faecalis bacteria. The findings of this study will open a better understanding of CsNPs and AuNPs as biomedical materials because of their remarkable qualities. [ABSTRACT FROM AUTHOR]