305 results on '"A. Peired"'
Search Results
2. Circulating inflammatory cytokines predict severity disease in hospitalized COVID-19 patients: A prospective multicenter study of the European DRAGON consortium
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Barbara Polese, Marie Ernst, Monique Henket, Benoit Ernst, Marie Winandy, Makon-Sébastien Njock, Céline Blockx, Stéphanie Kovacs, Florence Watar, Anna Julie Peired, Sara Tomassetti, Cosimo Nardi, Stéphanie Gofflot, Souad Rahmouni, James PR Schofield, Rebekah Penrice-Randal, Paul J. Skipp, Fabio Strazzeri, Erika Parkinson, Gilles Darcis, Benoit Misset, Michel Moutschen, Renaud Louis, Etienne Cavalier, and Julien Guiot
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COVID-19 ,Biomarker ,Disease severity ,Cytokines ,Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Background: COVID-19 has put a huge strain on the healthcare systems worldwide, requiring unprecedented intensive care resources. There is still an unmet clinical need for easily available biomarkers capable of predicting the risk for severe disease. The main goal of this prospective multicenter study was to identify biomarkers that could predict ICU admission and in-hospital mortality. Methods: We prospectively recruited COVID-19 PCR positive patients in two hospitals, in Belgium and Italy. Blood samples were collected at hospital admission and 20 potential biomarkers were measured with the Luminex technology. Logistic regression models were performed to identify the biomarkers that, alone or together, were associated with patient disease severity. Results: Our study demonstrates that elevated levels of circulating inflammatory cytokines were associated with disease severity in COVID-19 hospitalized patients. CXCL10, IL-4, IL-6 and MCP-1 values were predictive of ICU admission. Elevated levels of IL-6 and MCP-1 were also associated with in hospital death in COVID-19 hospitalized patients. Conclusion: Altogether, elevated and correlated inflammatory cytokines in the blood of COVID-19 patients at hospital admission are predictive of disease severity and suggest a dysregulated inflammation induced by SARS-CoV-2 infection.
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- 2024
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3. Circulating inflammatory cytokines predict severity disease in hospitalized COVID-19 patients: A prospective multicenter study of the European DRAGON consortium
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Polese, Barbara, Ernst, Marie, Henket, Monique, Ernst, Benoit, Winandy, Marie, Njock, Makon-Sébastien, Blockx, Céline, Kovacs, Stéphanie, Watar, Florence, Peired, Anna Julie, Tomassetti, Sara, Nardi, Cosimo, Gofflot, Stéphanie, Rahmouni, Souad, Schofield, James PR, Penrice-Randal, Rebekah, Skipp, Paul J., Strazzeri, Fabio, Parkinson, Erika, Darcis, Gilles, Misset, Benoit, Moutschen, Michel, Louis, Renaud, Cavalier, Etienne, and Guiot, Julien
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- 2024
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4. Anti-slit diaphragm antibodies on kidney biopsy identify pediatric patients with steroid-resistant nephrotic syndrome responsive to second-line immunosuppressants
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Raglianti, Valentina, Angelotti, Maria Lucia, Cirillo, Luigi, Ravaglia, Fiammetta, Landini, Samuela, Palazzo, Viviana, Melica, Maria Elena, Antonelli, Giulia, Conte, Carolina, Buti, Elisa, Errichiello, Carmela, De Chiara, Letizia, Peired, Anna Julie, Lasagni, Laura, Buccoliero, Anna Maria, Allinovi, Marco, Montero, Anna Manonelles, Cruzado, Josep Maria, Bruschi, Maurizio, Ghiggeri, Gian Marco, Angeletti, Andrea, Anders, Hans-Joachim, Lazzeri, Elena, Mazzinghi, Benedetta, Becherucci, Francesca, and Romagnani, Paola
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- 2024
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5. Modeled microgravity unravels the roles of mechanical forces in renal progenitor cell physiology
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Maria Elena Melica, Francesca Cialdai, Gilda La Regina, Chiara Risaliti, Tommaso Dafichi, Anna Julie Peired, Paola Romagnani, Monica Monici, and Laura Lasagni
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Modeled microgravity ,Renal progenitors ,Podocyte ,Cyclosporine A ,Mechanobiology ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background The glomerulus is a highly complex system, composed of different interdependent cell types that are subjected to various mechanical stimuli. These stimuli regulate multiple cellular functions, and changes in these functions may contribute to tissue damage and disease progression. To date, our understanding of the mechanobiology of glomerular cells is limited, with most research focused on the adaptive response of podocytes. However, it is crucial to recognize the interdependence between podocytes and parietal epithelial cells, in particular with the progenitor subset, as it plays a critical role in various manifestations of glomerular diseases. This highlights the necessity to implement the analysis of the effects of mechanical stress on renal progenitor cells. Methods Microgravity, modeled by Rotary Cell Culture System, has been employed as a system to investigate how renal progenitor cells respond to alterations in the mechanical cues within their microenvironment. Changes in cell phenotype, cytoskeleton organization, cell proliferation, cell adhesion and cell capacity for differentiation into podocytes were analyzed. Results In modeled microgravity conditions, renal progenitor cells showed altered cytoskeleton and focal adhesion organization associated with a reduction in cell proliferation, cell adhesion and spreading capacity. Moreover, mechanical forces appeared to be essential for renal progenitor differentiation into podocytes. Indeed, when renal progenitors were exposed to a differentiative agent in modeled microgravity conditions, it impaired the acquisition of a complex podocyte-like F-actin cytoskeleton and the expression of specific podocyte markers, such as nephrin and nestin. Importantly, the stabilization of the cytoskeleton with a calcineurin inhibitor, cyclosporine A, rescued the differentiation of renal progenitor cells into podocytes in modeled microgravity conditions. Conclusions Alterations in the organization of the renal progenitor cytoskeleton due to unloading conditions negatively affect the regenerative capacity of these cells. These findings strengthen the concept that changes in mechanical cues can initiate a pathophysiological process in the glomerulus, not only altering podocyte actin cytoskeleton, but also extending the detrimental effect to the renal progenitor population. This underscores the significance of the cytoskeleton as a druggable target for kidney diseases.
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- 2024
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6. Doubts and concerns about COVID-19 uncertainties on imaging data, clinical score, and outcomes
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Cosimo Nardi, Andrea Magnini, Linda Calistri, Edoardo Cavigli, Anna Julie Peired, Vieri Rastrelli, Edoardo Carlesi, Giulia Zantonelli, Olga Smorchkova, Lorenzo Cinci, Martina Orlandi, Nicholas Landini, Edoardo Berillo, Chiara Lorini, Jessica Mencarini, Maria Grazia Colao, Leonardo Gori, Valentina Luzzi, Chiara Lazzeri, Elisa Cipriani, Manuela Bonizzoli, Filippo Pieralli, Carlo Nozzoli, Alessandro Morettini, Federico Lavorini, Alessandro Bartoloni, Gian Maria Rossolini, Marco Matucci-Cerinic, Sara Tomassetti, and Stefano Colagrande
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COVID-19 ,SARS-CoV-2 ,Acute respiratory disease ,Pneumonia ,Computed tomography ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background COVID-19 is a pandemic disease affecting predominantly the respiratory apparatus with clinical manifestations ranging from asymptomatic to respiratory failure. Chest CT is a crucial tool in diagnosing and evaluating the severity of pulmonary involvement through dedicated scoring systems. Nonetheless, many questions regarding the relationship of radiologic and clinical features of the disease have emerged in multidisciplinary meetings. The aim of this retrospective study was to explore such relationship throughout an innovative and alternative approach. Materials and methods This study included 550 patients (range 25–98 years; 354 males, mean age 66.1; 196 females, mean age 70.9) hospitalized for COVID-19 with available radiological and clinical data between 1 March 2021 and 30 April 2022. Radiological data included CO-RADS, chest CT score, dominant pattern, and typical/atypical findings detected on CT examinations. Clinical data included clinical score and outcome. The relationship between such features was investigated through the development of the main four frequently asked questions summarizing the many issues arisen in multidisciplinary meetings, as follows 1) CO-RADS, chest CT score, clinical score, and outcomes; 2) the involvement of a specific lung lobe and outcomes; 3) dominant pattern/distribution and severity score for the same chest CT score; 4) additional factors and outcomes. Results 1) If CT was suggestive for COVID, a strong correlation between CT/clinical score and prognosis was found; 2) Middle lobe CT involvement was an unfavorable prognostic criterion; 3) If CT score 50%, crazy paving as dominant pattern leaded to a 15% increased death rate, stacked up against other patterns, thus almost doubling it; 4) Additional factors usually did not matter, but lymph-nodes and pleural effusion worsened prognosis. Conclusions This study outlined those radiological features of COVID-19 most relevant towards disease severity and outcome with an innovative approach.
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- 2023
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7. Utility of bronchoalveolar lavage for COVID-19: a perspective from the Dragon consortium
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Sara Tomassetti, Luca Ciani, Valentina Luzzi, Leonardo Gori, Marco Trigiani, Leonardo Giuntoli, Federico Lavorini, Venerino Poletti, Claudia Ravaglia, Alfons Torrego, Fabien Maldonado, Robert Lentz, Francesco Annunziato, Laura Maggi, Gian Maria Rossolini, Simona Pollini, Ombretta Para, Greta Ciurleo, Alessandro Casini, Laura Rasero, Alessandro Bartoloni, Michele Spinicci, Mohammed Munavvar, Stefano Gasparini, Camilla Comin, Marco Matucci Cerinic, Anna Peired, Monique Henket, Benoit Ernst, Renaud Louis, Jean-louis Corhay, Cosimo Nardi, and Julien Guiot
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COVID-19 ,bronchoalveolar lavage ,interstitial pneumonia ,infections ,interventional pulmonology ,Medicine (General) ,R5-920 - Abstract
Diagnosing COVID-19 and treating its complications remains a challenge. This review reflects the perspective of some of the Dragon (IMI 2-call 21, #101005122) research consortium collaborators on the utility of bronchoalveolar lavage (BAL) in COVID-19. BAL has been proposed as a potentially useful diagnostic tool to increase COVID-19 diagnosis sensitivity. In both critically ill and non-critically ill COVID-19 patients, BAL has a relevant role in detecting other infections or supporting alternative diagnoses and can change management decisions in up to two-thirds of patients. BAL is used to guide steroid and immunosuppressive treatment and to narrow or discontinue antibiotic treatment, reducing the use of unnecessary broad antibiotics. Moreover, cellular analysis and novel multi-omics techniques on BAL are of critical importance for understanding the microenvironment and interaction between epithelial cells and immunity, revealing novel potential prognostic and therapeutic targets. The BAL technique has been described as safe for both patients and healthcare workers in more than a thousand procedures reported to date in the literature. Based on these preliminary studies, we recognize that BAL is a feasible procedure in COVID-19 known or suspected cases, useful to properly guide patient management, and has great potential for research.
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- 2024
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8. Doubts and concerns about COVID-19 uncertainties on imaging data, clinical score, and outcomes
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Nardi, Cosimo, Magnini, Andrea, Calistri, Linda, Cavigli, Edoardo, Peired, Anna Julie, Rastrelli, Vieri, Carlesi, Edoardo, Zantonelli, Giulia, Smorchkova, Olga, Cinci, Lorenzo, Orlandi, Martina, Landini, Nicholas, Berillo, Edoardo, Lorini, Chiara, Mencarini, Jessica, Colao, Maria Grazia, Gori, Leonardo, Luzzi, Valentina, Lazzeri, Chiara, Cipriani, Elisa, Bonizzoli, Manuela, Pieralli, Filippo, Nozzoli, Carlo, Morettini, Alessandro, Lavorini, Federico, Bartoloni, Alessandro, Rossolini, Gian Maria, Matucci-Cerinic, Marco, Tomassetti, Sara, and Colagrande, Stefano
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- 2023
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9. Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease
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Letizia De Chiara, Carolina Conte, Roberto Semeraro, Paula Diaz-Bulnes, Maria Lucia Angelotti, Benedetta Mazzinghi, Alice Molli, Giulia Antonelli, Samuela Landini, Maria Elena Melica, Anna Julie Peired, Laura Maggi, Marta Donati, Gilda La Regina, Marco Allinovi, Fiammetta Ravaglia, Daniele Guasti, Daniele Bani, Luigi Cirillo, Francesca Becherucci, Francesco Guzzi, Alberto Magi, Francesco Annunziato, Laura Lasagni, Hans-Joachim Anders, Elena Lazzeri, and Paola Romagnani
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Science - Abstract
Acute kidney injury is frequent, often fatal and can leave survivors with chronic kidney disease. Here the authors show that tubular cell polyploidy reduces early fatality sustaining residual function but promotes chronic kidney disease, which can be prevented by blocking YAP1
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- 2022
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10. Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes
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Maria Elena Melica, Maria Lucia Angelotti, Giulia Antonelli, Anna Peired, Carolina Conte, Letizia De Chiara, Benedetta Mazzinghi, Elena Lazzeri, Laura Lasagni, and Paola Romagnani
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Biology (General) ,QH301-705.5 - Abstract
Kidney diseases are a global health concern. Modeling of kidney disease for translational research is often challenging because of species specificities or the postmitotic status of kidney epithelial cells that make primary cultures, for example podocytes. Here, we report a protocol for preparing primary cultures of podocytes based on the isolation and in vitro propagation of immature kidney progenitor cells subsequently differentiated into mature podocytes. This protocol can be useful for studying physiology and pathophysiology of human kidney progenitors and to obtain differentiated podocytes for modeling podocytopathies and other kidney disorders involving podocytes.Graphical overview
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- 2023
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11. Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease
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De Chiara, Letizia, Conte, Carolina, Semeraro, Roberto, Diaz-Bulnes, Paula, Angelotti, Maria Lucia, Mazzinghi, Benedetta, Molli, Alice, Antonelli, Giulia, Landini, Samuela, Melica, Maria Elena, Peired, Anna Julie, Maggi, Laura, Donati, Marta, La Regina, Gilda, Allinovi, Marco, Ravaglia, Fiammetta, Guasti, Daniele, Bani, Daniele, Cirillo, Luigi, Becherucci, Francesca, Guzzi, Francesco, Magi, Alberto, Annunziato, Francesco, Lasagni, Laura, Anders, Hans-Joachim, Lazzeri, Elena, and Romagnani, Paola
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- 2022
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12. From kidney injury to kidney cancer
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Peired, Anna Julie, Lazzeri, Elena, Guzzi, Francesco, Anders, Hans-Joachim, and Romagnani, Paola
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- 2021
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13. The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs
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Agnieszka Smolinska, David S. Jessop, Kirk L. Pappan, Alexandra De Saedeleer, Amerjit Kang, Alexandra L. Martin, Max Allsworth, Charlotte Tyson, Martine P. Bos, Matt Clancy, Mike Morel, Tony Cooke, Tom Dymond, Claire Harris, Jacqui Galloway, Paul Bresser, Nynke Dijkstra, Viresh Jagesar, Paul H. M. Savelkoul, Erik V. H. Beuken, Wesley H. V. Nix, Renaud Louis, Muriel Delvaux, Doriane Calmes, Benoit Ernst, Simona Pollini, Anna Peired, Julien Guiot, Sara Tomassetti, Andries E. Budding, Frank McCaughan, Stefan J. Marciniak, and Marc P. van der Schee
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Medicine ,Science - Abstract
Abstract Face masks and personal respirators are used to curb the transmission of SARS-CoV-2 in respiratory droplets; filters embedded in some personal protective equipment could be used as a non-invasive sample source for applications, including at-home testing, but information is needed about whether filters are suited to capture viral particles for SARS-CoV-2 detection. In this study, we generated inactivated virus-laden aerosols of 0.3–2 microns in diameter (0.9 µm mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100 copies/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. The ability to detect nucleic acids on face mask filters may, however, find other uses worth future investigation.
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- 2021
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14. Edema-like marrow signal intensity: a narrative review with a pictorial essay
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Maraghelli, Davide, Brandi, Maria Luisa, Matucci Cerinic, Marco, Peired, Anna Julie, and Colagrande, Stefano
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- 2021
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15. An urgent call for environmental accountability in nephrology clinical trials.
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Peired, Anna Julie, Laranjinha, Ivo, Demirtas, Gulay, Ferreira, Ana Carina, Gracin, Sonja, Knoeller, Susi, and Hourmant, Maryvonne
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PATIENTS' attitudes , *PATIENT experience , *MANAGEMENT of medical records , *MEDICAL personnel , *MEDICAL societies , *MANAGEMENT of electronic health records , *NEPHROLOGISTS - Abstract
The article discusses the urgent need for environmental accountability in nephrology clinical trials. It highlights the significant carbon dioxide equivalent (CO2e) emissions associated with clinical trials and emphasizes the lack of studies assessing the environmental impact of these trials. The article suggests various strategies to mitigate the environmental effects, such as optimizing trial site locations, reducing unnecessary data and sample collection, favoring local lab work, and minimizing travel for patients and the trial team. It also explores the potential benefits of digitalization, artificial intelligence (AI), and machine learning in improving the sustainability of clinical trials. The article calls for collective efforts from the nephrology community, including researchers, pharmaceutical companies, and funding bodies, to prioritize sustainability in clinical trials. [Extracted from the article]
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- 2024
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16. Laboratory data and broncho-alveolar lavage on Covid-19 patients with no intensive care unit admission: Correlation with chest CT features and clinical outcomes.
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Nardi, Cosimo, Magnini, Andrea, Rastrelli, Vieri, Zantonelli, Giulia, Calistri, Linda, Lorini, Chiara, Luzzi, Valentina, Gori, Leonardo, Ciani, Luca, Morecchiato, Fabio, Simonetti, Virginia, Peired, Anna Julie, Landini, Nicholas, Cavigli, Edoardo, Yang, Guang, Guiot, Julien, Tomassetti, Sara, and Colagrande, Stefano
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- 2024
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17. Utility of bronchoalveolar lavage for COVID-19: a perspective from the Dragon consortium
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Tomassetti, Sara, primary, Ciani, Luca, additional, Luzzi, Valentina, additional, Gori, Leonardo, additional, Trigiani, Marco, additional, Giuntoli, Leonardo, additional, Lavorini, Federico, additional, Poletti, Venerino, additional, Ravaglia, Claudia, additional, Torrego, Alfons, additional, Maldonado, Fabien, additional, Lentz, Robert, additional, Annunziato, Francesco, additional, Maggi, Laura, additional, Rossolini, Gian Maria, additional, Pollini, Simona, additional, Para, Ombretta, additional, Ciurleo, Greta, additional, Casini, Alessandro, additional, Rasero, Laura, additional, Bartoloni, Alessandro, additional, Spinicci, Michele, additional, Munavvar, Mohammed, additional, Gasparini, Stefano, additional, Comin, Camilla, additional, Cerinic, Marco Matucci, additional, Peired, Anna, additional, Henket, Monique, additional, Ernst, Benoit, additional, Louis, Renaud, additional, Corhay, Jean-louis, additional, Nardi, Cosimo, additional, and Guiot, Julien, additional
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- 2024
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18. Increased KL-6 levels in moderate to severe COVID-19 infection.
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Maureen Cambier, Monique Henket, Anne Noelle Frix, Stéphanie Gofflot, Marie Thys, Sara Tomasetti, Anna Peired, Ingrid Struman, Anne-Françoise Rousseau, Benoît Misset, Gilles Darcis, Michel Moutschen, Renaud Louis, Makon-Sébastien Njock, Etienne Cavalier, and Julien Guiot
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Medicine ,Science - Abstract
BackgroundThe global coronavirus disease 2019 (COVID-19) has presented significant challenges and created concerns worldwide. Besides, patients who have experienced a SARS-CoV-2 infection could present post-viral complications that can ultimately lead to pulmonary fibrosis. Serum levels of Krebs von den Lungen 6 (KL-6), high molecular weight human MUC1 mucin, are increased in the most patients with various interstitial lung damage. Since its production is raised during epithelial damages, KL-6 could be a helpful non-invasive marker to monitor COVID-19 infection and predict post-infection sequelae.MethodsWe retrospectively evaluated KL-6 levels of 222 COVID-19 infected patients and 70 healthy control. Serum KL-6, fibrinogen, lactate dehydrogenase (LDH), platelet-lymphocytes ratio (PLR) levels and other biological parameters were analyzed. This retrospective study also characterized the relationships between serum KL-6 levels and pulmonary function variables.ResultsOur results showed that serum KL-6 levels in COVID-19 patients were increased compared to healthy subjects (470 U/ml vs 254 U/ml, P 453.5 U/ml was associated with COVID-19 (AUC = 0.8415, P < 0.0001). KL-6 level was positively correlated with other indicators of disease severity such as fibrinogen level (r = 0.1475, P = 0.0287), LDH level (r = 0,31, P = 0,004) and PLR level (r = 0.23, P = 0.0005). However, KL-6 levels were not correlated with pulmonary function tests (r = 0.04, P = 0.69).ConclusionsKL-6 expression was correlated with several disease severity indicators. However, the association between mortality and long-term follow-up outcomes needs further investigation. More extensive trials are required to prove that KL-6 could be a marker of disease severity in COVID-19 infection.
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- 2022
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19. The SARS-CoV-2 viral load in COVID-19 patients is lower on face mask filters than on nasopharyngeal swabs
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Smolinska, Agnieszka, Jessop, David S., Pappan, Kirk L., De Saedeleer, Alexandra, Kang, Amerjit, Martin, Alexandra L., Allsworth, Max, Tyson, Charlotte, Bos, Martine P., Clancy, Matt, Morel, Mike, Cooke, Tony, Dymond, Tom, Harris, Claire, Galloway, Jacqui, Bresser, Paul, Dijkstra, Nynke, Jagesar, Viresh, Savelkoul, Paul H. M., Beuken, Erik V. H., Nix, Wesley H. V., Louis, Renaud, Delvaux, Muriel, Calmes, Doriane, Ernst, Benoit, Pollini, Simona, Peired, Anna, Guiot, Julien, Tomassetti, Sara, Budding, Andries E., McCaughan, Frank, Marciniak, Stefan J., and van der Schee, Marc P.
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- 2021
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20. Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury
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Elena Lazzeri, Maria Lucia Angelotti, Anna Peired, Carolina Conte, Julian A. Marschner, Laura Maggi, Benedetta Mazzinghi, Duccio Lombardi, Maria Elena Melica, Sara Nardi, Elisa Ronconi, Alessandro Sisti, Giulia Antonelli, Francesca Becherucci, Letizia De Chiara, Ricardo Romero Guevara, Alexa Burger, Beat Schaefer, Francesco Annunziato, Hans-Joachim Anders, Laura Lasagni, and Paola Romagnani
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Science - Abstract
The recovery of function upon acute kidney injury is thought to involve tubular cell dedifferentiation and proliferation. Here the authors show that Pax2+ progenitors regenerate tubules via cell division while other tubular cells support function recovery by undergoing hypertrophy through endoreplication.
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- 2018
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21. Extensive biomarker analysis in a European cohort study of patients suffering from COVID-19: a perspective from the DRAGON consortium
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Guiot, Julien, primary, Ernst, Marie, additional, Maes, Nathalie, additional, Henket, Monique, additional, Peired, Anna, additional, Ernst, Benoit, additional, Tomassetti, Sara, additional, and Cavalier, Etienne, additional
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- 2023
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22. Polyploid tubular cells initiate a TGF-β1 controlled loop that sustains polyploidization and fibrosis after acute kidney injury
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De Chiara, Letizia, primary, Semeraro, Roberto, additional, Mazzinghi, Benedetta, additional, Landini, Samuela, additional, Molli, Alice, additional, Antonelli, Giulia, additional, Angelotti, Maria Lucia, additional, Melica, Maria Elena, additional, Maggi, Laura, additional, Conte, Carolina, additional, Peired, Anna Julie, additional, Cirillo, Luigi, additional, Raglianti, Valentina, additional, Magi, Alberto, additional, Annunziato, Francesco, additional, Romagnani, Paola, additional, and Lazzeri, Elena, additional
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- 2023
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23. Role of Sex Hormones in Prevalent Kidney Diseases
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Conte, Carolina, primary, Antonelli, Giulia, additional, Melica, Maria Elena, additional, Tarocchi, Mirko, additional, Romagnani, Paola, additional, and Peired, Anna Julie, additional
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- 2023
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24. Molecular Mechanisms of Renal Progenitor Regulation: How Many Pieces in the Puzzle?
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Anna Julie Peired, Maria Elena Melica, Alice Molli, Cosimo Nardi, Paola Romagnani, and Laura Lasagni
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renal progenitors ,molecular mechanisms ,kidney injury ,single-cell RNA sequencing ,molecular signature ,Cytology ,QH573-671 - Abstract
Kidneys of mice, rats and humans possess progenitors that maintain daily homeostasis and take part in endogenous regenerative processes following injury, owing to their capacity to proliferate and differentiate. In the glomerular and tubular compartments of the nephron, consistent studies demonstrated that well-characterized, distinct populations of progenitor cells, localized in the parietal epithelium of Bowman capsule and scattered in the proximal and distal tubules, could generate segment-specific cells in physiological conditions and following tissue injury. However, defective or abnormal regenerative responses of these progenitors can contribute to pathologic conditions. The molecular characteristics of renal progenitors have been extensively studied, revealing that numerous classical and evolutionarily conserved pathways, such as Notch or Wnt/β-catenin, play a major role in cell regulation. Others, such as retinoic acid, renin-angiotensin-aldosterone system, TLR2 (Toll-like receptor 2) and leptin, are also important in this process. In this review, we summarize the plethora of molecular mechanisms directing renal progenitor responses during homeostasis and following kidney injury. Finally, we will explore how single-cell RNA sequencing could bring the characterization of renal progenitors to the next level, while knowing their molecular signature is gaining relevance in the clinic.
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- 2021
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25. Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes
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Melica, Maria, primary, Angelotti, Maria, additional, Antonelli, Giulia, additional, Peired, Anna, additional, Conte, Carolina, additional, Chiara, Letizia, additional, Mazzinghi, Benedetta, additional, Lazzeri, Elena, additional, Lasagni, Laura, additional, and Romagnani, Paola, additional
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- 2023
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26. Podocyte Regeneration Driven by Renal Progenitors Determines Glomerular Disease Remission and Can Be Pharmacologically Enhanced
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Lasagni, Laura, Angelotti, Maria Lucia, Ronconi, Elisa, Lombardi, Duccio, Nardi, Sara, Peired, Anna, Becherucci, Francesca, Mazzinghi, Benedetta, Sisti, Alessandro, Romoli, Simone, Burger, Alexa, Schaefer, Beat, Buccoliero, Annamaria, Lazzeri, Elena, and Romagnani, Paola
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- 2015
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27. Podocyte Regeneration Driven by Renal Progenitors Determines Glomerular Disease Remission and Can Be Pharmacologically Enhanced
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Laura Lasagni, Maria Lucia Angelotti, Elisa Ronconi, Duccio Lombardi, Sara Nardi, Anna Peired, Francesca Becherucci, Benedetta Mazzinghi, Alessandro Sisti, Simone Romoli, Alexa Burger, Beat Schaefer, Annamaria Buccoliero, Elena Lazzeri, and Paola Romagnani
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Podocyte loss is a general mechanism of glomerular dysfunction that initiates and drives the progression of chronic kidney disease, which affects 10% of the world population. Here, we evaluate whether the regenerative response to podocyte injury influences chronic kidney disease outcome. In models of focal segmental glomerulosclerosis performed in inducible transgenic mice where podocytes are tagged, remission or progression of disease was determined by the amount of regenerated podocytes. When the same model was established in inducible transgenic mice where renal progenitors are tagged, the disease remitted if renal progenitors successfully differentiated into podocytes, while it persisted if differentiation was ineffective, resulting in glomerulosclerosis. Treatment with BIO, a GSK3s inhibitor, significantly increased disease remission by enhancing renal progenitor sensitivity to the differentiation effect of endogenous retinoic acid. These results establish renal progenitors as critical determinants of glomerular disease outcome and a pharmacological enhancement of their differentiation as a possible therapeutic strategy.
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- 2015
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28. Increased KL-6 levels in moderate to severe COVID-19 infection
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Cambier, Maureen, primary, Henket, Monique, additional, Frix, Anne Noelle, additional, Gofflot, Stéphanie, additional, Thys, Marie, additional, Tomasetti, Sara, additional, Peired, Anna, additional, Struman, Ingrid, additional, Rousseau, Anne-Françoise, additional, Misset, Benoît, additional, Darcis, Gilles, additional, Moutschen, Michel, additional, Louis, Renaud, additional, Njock, Makon-Sébastien, additional, Cavalier, Etienne, additional, and Guiot, Julien, additional
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- 2022
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29. Phenotypes of Post-COVID-19 Interstitial Lung Disease: Clinical, Radiological, and Pathological Correlations
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Gori, Leonardo, primary, Ravaglia, Claudia, additional, Luzzi, Valentina, additional, Ciani, Luca, additional, Biadene, Giulia, additional, Bambina, Sonia, additional, Marinato, Martina, additional, Dubini, Alessandra, additional, Cozzi, Diletta, additional, Cavigli, Edoardo, additional, Comin, Camilla, additional, Pasini, Valeria, additional, Puglisi, Silvia, additional, Cavazza, Alberto, additional, Poletti, Venerino, additional, Spinicc, Michele, additional, Bartoloni, Alessandro, additional, Peired, Anna, additional, Ferraro, Stefania, additional, Papa, Giulia Caterina, additional, Berillo, Edoardo, additional, Nardi, Cosimo, additional, Rosi, Elisabetta, additional, Lavorini, Federico, additional, and Tomassett, Sara, additional
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- 2022
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30. Telomerase activated thymidine analogue pro-drug is a new molecule targeting hepatocellular carcinoma
- Author
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Tarocchi, Mirko, Polvani, Simone, Peired, Anna Julie, Marroncini, Giada, Calamante, Massimo, Ceni, Elisabetta, Rhodes, Daniela, Mello, Tommaso, Pieraccini, Giuseppe, Quattrone, Alessandro, Luchinat, Claudio, and Galli, Andrea
- Published
- 2014
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31. Retinoids and Glomerular Regeneration
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Lazzeri, Elena, Peired, Anna Julie, Lasagni, Laura, and Romagnani, Paola
- Published
- 2014
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32. From kidney injury to kidney cancer
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Hans-Joachim Anders, Paola Romagnani, Francesco Guzzi, Elena Lazzeri, and Anna Julie Peired
- Subjects
0301 basic medicine ,030232 urology & nephrology ,Kidney ,Bioinformatics ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Renal Insufficiency, Chronic ,Risk factor ,urogenital system ,business.industry ,Acute kidney injury ,Cancer ,Acute Kidney Injury ,medicine.disease ,Kidney Neoplasms ,Pathophysiology ,3. Good health ,030104 developmental biology ,medicine.anatomical_structure ,Clinical research ,Nephrology ,acute kidney injury ,chronic kidney disease ,kidney cancer ,risk factor ,surgery ,survival ,Neoplasm Recurrence, Local ,business ,Kidney cancer ,Kidney disease - Abstract
Epidemiologic studies document strong associations between acute or chronic kidney injury and kidney tumors. However, whether these associations are linked by causation, and in which direction, is unclear. Accumulating data from basic and clinical research now shed light on this issue and prompt us to propose a new pathophysiological concept with immanent implications in the management of patients with kidney disease and patients with kidney tumors. As a central paradigm, this review proposes the mechanisms of kidney damage and repair that are active during acute kidney injury but also during persistent injuries in chronic kidney disease as triggers of DNA damage, promoting the expansion of (pre-)malignant cell clones. As renal progenitors have been identified by different studies as the cell of origin for several benign and malignant kidney tumors, we discuss how the different types of kidney tumors relate to renal progenitors at specific sites of injury and to germline or somatic mutations in distinct signaling pathways. We explain how known risk factors for kidney cancer rather represent risk factors for kidney injury as an upstream cause of cancer. Finally, we propose a new role for nephrologists in kidney cancer (i.e., the primary and secondary prevention and treatment of kidney injury to reduce incidence, prevalence, and recurrence of kidney cancer).
- Published
- 2021
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33. List of Contributors
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Abecassis, Michael, primary, Abed, Ahmed, additional, Abelli, Massimo, additional, Ahmad, Sarwat, additional, Alessiani, Mario, additional, Alsharidi, Aynaa, additional, Altruda, Fiorella, additional, Amorese, Gabriella, additional, Angeletti, Andrea, additional, Angelico, Mario, additional, Angelico, Roberta, additional, Angelotti, Maria L., additional, Applegate, Robert J., additional, Atala, Anthony, additional, Attanasio, Chiara, additional, Axelrod, David, additional, Bababekov, Yanik, additional, Basu, Joydeep, additional, Becherucci, Francesca, additional, Belford, P. Matthew, additional, Benedetti, Enrico, additional, Benedetti, Valentina, additional, Benigni, Ariela, additional, Bertram, Timothy A., additional, Bestard, Oriol, additional, Blake, Joshua, additional, Boggi, Ugo, additional, Brasile, Lauren, additional, Bromberg, Jonathan S., additional, Brouard, Sophie, additional, Brovold, Matthew, additional, Burke, George W., additional, Busic, Mirela, additional, Butt, Zeeshan, additional, Caddeo, Silvia, additional, Calzone, Stefano, additional, Campistol, Josep M., additional, Carmagnola, Irene, additional, Carty, Fiona, additional, Castanares-Zapatero, Diego, additional, Chadjichristos, Christos E., additional, Chambers, Brooke E., additional, Chandran, Sindhu, additional, Chatziantoniou, Christos, additional, Chen, Ashton, additional, Chen, Linda, additional, Chen, Xiwu, additional, Chiono, Valeria, additional, Chiusa, Manuel, additional, Ciancio, Gaetano, additional, Ciardelli, Gianluca, additional, Coletti, Gino, additional, Coll, Elisabeth, additional, Collienne, Christine, additional, Colvin, Robert B., additional, Cortinovis, Monica, additional, Cosimi, A. Benedict, additional, Cravedi, Paolo, additional, D’Amico, Giuseppe, additional, Da Sacco, Stefano, additional, Danger, Richard, additional, Dantal, Jacques, additional, Davidson, Alan J., additional, De Chiara, Letizia, additional, De Fijter, Johannes W., additional, de la Rosa, Gloria, additional, Delmonico, Francis L., additional, Deng, Junhong, additional, Deurdulian, Corinne, additional, Dhal, Abritee, additional, Dionigi, Paolo, additional, Domínguez-Gil, Beatriz, additional, Drozdzik, Marek, additional, Dussaule, Jean-Claude, additional, Edgar, Lauren, additional, Egidi, Maria Francesca, additional, El Hennawy, Hany, additional, English, Karen, additional, Everly, Matthew J., additional, Fagoonee, Sharmila, additional, Famulari, Elvira Smeralda, additional, Farney, Alan C., additional, Figliuzzi, Marina, additional, Framarino-dei-Malatesta, Marialuisa, additional, Fumo, David E., additional, Gagliardini, Elena, additional, Gallon, Lorenzo, additional, Gandhi, Sanjay K., additional, Gautreaux, Michael D., additional, Geraedts, Anna, additional, Giannese, Domenico, additional, Giulianotti, Pier C., additional, Goligorsky, Michael S., additional, Griesemar, Adam, additional, Grinyó, Josep M., additional, Gruessner, Angelika C., additional, Gruessner, Rainer W.G., additional, He, Bulang, additional, Heher, Eliot, additional, Ho, Bing, additional, Hosgood, Sarah A., additional, Hotta, Kiyohiko, additional, Humar, Atul, additional, Humes, H. David, additional, Iaria, Giuseppe, additional, Imberti, Barbara, additional, Izpisua Belmonte, Juan Carlos, additional, Jochmans, Ina, additional, Katari, Ravi, additional, Kavvadas, Panagiotis, additional, Kawai, Tatsuo, additional, Kengla, Carlos, additional, Keys, Tristan, additional, Kidiyoor, Amritha, additional, Kidokoro, Kengo, additional, Kumar, Deepali, additional, Kutcher, Michael A., additional, Lai, Quirino, additional, Laterre, Pierre-François, additional, Lebbé, Céleste, additional, Legendre, Christophe, additional, Lennon, Rachel, additional, Li, Peng, additional, Lin, Jen-Jar, additional, Little, Melissa H., additional, Lu, Xiongbing, additional, Ludlow, John W., additional, Mahíllo, Beatriz, additional, Masereeuw, Rosalinde, additional, Matesanz, Rafael, additional, Matovinovic, Mirjana S., additional, Mazzinghi, Benedetta, additional, Mbiandjeu Toya, Serge Cedrick, additional, McEwen, Scott, additional, Melandro, Fabio, additional, Melchiorri, Loredana, additional, Menon, Madhav C., additional, Mirzazadeh, Majid, additional, Montag, Samantha, additional, Montgomery, Robert A., additional, Montiel, Virginie, additional, Montserrat, Nuria, additional, Morigi, Marina, additional, Morrill, Christian C., additional, Mourad, Michel, additional, Murphy, Sean V., additional, Murray, Patricia, additional, Nardo, Tiziana, additional, Netti, Paolo A., additional, Nguyen, Mark, additional, Nicholson, Michael L., additional, O’Callaghan, John M., additional, Ohler, Linda, additional, Orlando, Giuseppe, additional, Osafune, Kenji, additional, Oura, Tetsu, additional, Peired, Anna, additional, Peloso, Andrea, additional, Peng, Zhenzhen, additional, Perico, Norberto, additional, Perin, Laura, additional, Perrone, Vittorio, additional, Persad, Paul, additional, Peti-Peterdi, János, additional, Petrosyan, Astgik, additional, Pietrabissa, Andrea, additional, Pino, Christopher J., additional, Pirenne, Jacques, additional, Pisani, Francesco, additional, Ploeg, Rutger J., additional, Porrini, Esteban, additional, Pozzi, Ambra, additional, Pugliese, Alberto, additional, Pugliese, Luigi, additional, Rabelink, Ton J., additional, Rampino, Teresa, additional, Rees, Michael A., additional, Reinders, Marlies E.J., additional, Remuzzi, Andrea, additional, Remuzzi, Giuseppe, additional, Riquier-Brison, Anne, additional, Roca, Raquel G., additional, Rogers, Jeffrey, additional, Romagnani, Paola, additional, Rosales, Ivy A., additional, Rosenblum, Norman D., additional, Rota, Cinzia, additional, Ruggenenti, Piero, additional, Ruini, Francesca, additional, Sageshima, Junichiro, additional, El Salem, Fadi, additional, Sandal, Shaifali, additional, Sander, Veronika, additional, Santeramo, Ilaria, additional, Santos, Renato M., additional, Sarwal, Minnie, additional, Shah, Jigesh, additional, Shetty, Aneesha A., additional, Silengo, Lorenzo, additional, Siskind, Eric, additional, Skaro, Anton, additional, Snanoudj, Renaud, additional, Soker, Shay, additional, South, Andrew M., additional, Spasovski, Goce, additional, Stratta, Robert J., additional, Strom, Charles, additional, Stubenitsky, Bart, additional, Tamburrini, Riccardo, additional, Tang, Qizhi, additional, Tantissattamo, Ekamol, additional, Tasaki, Masayuki, additional, Tchelepi, Hisham, additional, Ticozzelli, Elena, additional, Traitanon, Opas, additional, Trillini, Matias, additional, Tzvetanov, Ivo, additional, Valentín, María O., additional, Vincenti, Flavio, additional, Vistoli, Fabio, additional, Vriend, Jelle, additional, Walker, Stephen J., additional, Wertheim, Jason A., additional, Williams, David F., additional, Wilm, Bettina, additional, Wilmer, Martijn J., additional, Wingert, Rebecca A., additional, Wittebole, Xavier, additional, Xia, Yun, additional, Xinaris, Christodoulos, additional, Yamada, Kazuhiko, additional, Yokoo, Takashi, additional, Yokote, Shinya, additional, Zandvliet, Maarten L., additional, Zent, Roy, additional, Zhang, Yuanyuan, additional, Zhao, David X., additional, Zhao, Lihui, additional, and Zhao, Susan Y., additional
- Published
- 2017
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34. Principles of Kidney Regeneration
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Angelotti, Maria L., primary, Becherucci, Francesca, additional, Mazzinghi, Benedetta, additional, Peired, Anna, additional, and Romagnani, Paola, additional
- Published
- 2017
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35. Role of BAL in COVID-19 patients: a prospective multicentre study
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L Ciani, J Guiot, C Ravaglia, V Poletti, V Luzzi, L Giuntoli, L Gori, E Benoit, E Berillo, A Morettini, C Nozzoli, F Lavorini, A Peired, C Nardi, F Morecchiato, G M Rossolini, S Pollini, L Maggi, F Annunziato, M Matucci Cerinic, and S Tomassetti
- Published
- 2022
- Full Text
- View/download PDF
36. Phenotypes of post covid interstitial lung disease; clinical, radiological, and pathological correlations
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L Gori, C Ravaglia, V Luzzi, L Ciani, L Giuntoli, G Biadene, S Bambina, M Marinato, A Dubini, D Cozzi, E Cavigli, C Comin, V Pasini, S Puglisi, A Cavazza, V Poletti, M Spinicci, A Bartoloni, A Peired, S Ferraro, G Papa, E Berillo, C Nardi, E Rosi, F Lavorini, and S Tomassetti
- Published
- 2022
- Full Text
- View/download PDF
37. Utility of bronchoalveolar lavage for COVID-19: a perspective from the Dragon consortium
- Author
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Sara Tomassetti, Luca Ciani, Valentina Luzzi, Leonardo Gori, Leonardo Giuntoli, Federico Lavorini, Venerino Poletti, Claudia Ravaglia, Alfons Torrego, Fabien Maldonado, Robert Lentz, Francesco Annunziato, Laura Maggi, Gian Maria Rossolini, Simona Pollini, Ombretta Para, Greta Ciurleo, Alessandro Casini, Laura Rasero, Alessandro Bartoloni, Michele Spinicci, Mohammed Munavvar, Stefano Gasparini, Camilla Comin, Marco Matucci Cerinic, Anna Peired, Monique Henket, Ernst Benoit, Renaud Louis, Jean-Louis Corhay, Cosimo Nardi, and Julien Guiot
- Abstract
Diagnosing COVID-19 and treating its complications remains a challenge. This review reflects the perspective of some of the Dragon (IMI 2-call 21, #101005122) research consortium collaborators on the utility of bronchoalveolar lavage (BAL) in COVID-19. BAL has been proposed as a potentially useful diagnostic tool to increase COVID-19 diagnosis sensitivity. In both critically ill and non-critically ill COVID-19 patients, BAL has a relevant role in detecting other infections or in supporting alternative diagnosis, and can change management decisions in up to two-third of patients. BAL is used to guide steroid and immunosuppressive treatment and to narrow or discontinue antibiotic treatment reducing the use of unnecessary broad antibiotics. Moreover, cellular analysis and novel multi-omics techniques on BAL are of critical importance for the understanding of the microenvironment and interaction between epithelial cells and immunity revealing novel potential prognostic and therapeutic targets. The BAL technique has been described as safe for both patients and health care workers in more than a thousand procedures reported to date in the literature. Based on these preliminary studies, we recognize that BAL is a feasible procedure in COVID-19 known or suspected cases, useful to properly guide patient management and with great potential for research.
- Published
- 2022
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38. Differentiation of crescent-forming kidney progenitor cells into podocytes attenuates severe glomerulonephritis in mice
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Maria Elena Melica, Giulia Antonelli, Roberto Semeraro, Maria Lucia Angelotti, Gianmarco Lugli, Samuela Landini, Fiammetta Ravaglia, Gilda La Regina, Carolina Conte, Letizia De Chiara, Anna Julie Peired, Benedetta Mazzinghi, Marta Donati, Alice Molli, Stefanie Steiger, Alberto Magi, Niccolò Bartalucci, Valentina Raglianti, Francesco Guzzi, Laura Maggi, Francesco Annunziato, Alexa Burger, Elena Lazzeri, Hans-Joachim Anders, Laura Lasagni, and Paola Romagnani
- Subjects
Disease Models, Animal ,Mice ,Glomerulonephritis ,Podocytes ,Stem Cells ,Panobinostat ,Animals ,Humans ,General Medicine ,Kidney ,Article - Abstract
Crescentic glomerulonephritis is characterized by vascular necrosis and parietal epithelial cell hyperplasia in the space surrounding the glomerulus, resulting in the formation of crescents. Little is known about the molecular mechanisms driving this process. Inducing crescentic glomerulonephritis in two Pax2Cre reporter mouse models revealed that crescents derive from clonal expansion of single immature parietal epithelial cells. Preemptive and delayed histone deacetylase inhibition with panobinostat, a drug used to treat hematopoietic stem cell disorders, attenuated crescentic glomerulonephritis with recovery of kidney function in the two mouse models. Three-dimensional confocal microscopy and stimulated emission depletion superresolution imaging of mouse glomeruli showed that, in addition to exerting an anti-inflammatory and immunosuppressive effect, panobinostat induced differentiation of an immature hyperplastic parietal epithelial cell subset into podocytes, thereby restoring the glomerular filtration barrier. Single-cell RNA sequencing of human renal progenitor cells in vitro identified an immature stratifin-positive cell subset and revealed that expansion of this stratifin-expressing progenitor cell subset was associated with a poor outcome in human crescentic glomerulonephritis. Treatment of human parietal epithelial cells in vitro with panobinostat attenuated stratifin expression in renal progenitor cells, reduced their proliferation, and promoted their differentiation into podocytes. These results offer mechanistic insights into the formation of glomerular crescents and demonstrate that selective targeting of renal progenitor cells can attenuate crescent formation and the deterioration of kidney function in crescentic glomerulonephritis in mice.
- Published
- 2022
39. Phenotypes of post covid interstitial lung disease; clinical, radiological, and pathological correlations.
- Author
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Gori, L, primary, Ravaglia, C, additional, Luzzi, V, additional, Ciani, L, additional, Giuntoli, L, additional, Biadene, G, additional, Bambina, S, additional, Marinato, M, additional, Dubini, A, additional, Cozzi, D, additional, Cavigli, E, additional, Comin, C, additional, Pasini, V, additional, Puglisi, S, additional, Cavazza, A, additional, Poletti, V, additional, Spinicci, M, additional, Bartoloni, A, additional, Peired, A, additional, Ferraro, S, additional, Papa, G, additional, Berillo, E, additional, Nardi, C, additional, Rosi, E, additional, Lavorini, F, additional, and Tomassetti, S, additional
- Published
- 2022
- Full Text
- View/download PDF
40. Role of BAL in COVID-19 patients: a prospective multicentre study
- Author
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Ciani, L, primary, Guiot, J, additional, Ravaglia, C, additional, Poletti, V, additional, Luzzi, V, additional, Giuntoli, L, additional, Gori, L, additional, Benoit, E, additional, Berillo, E, additional, Morettini, A, additional, Nozzoli, C, additional, Lavorini, F, additional, Peired, A, additional, Nardi, C, additional, Morecchiato, F, additional, Rossolini, G M, additional, Pollini, S, additional, Maggi, L, additional, Annunziato, F, additional, Matucci Cerinic, M, additional, and Tomassetti, S, additional
- Published
- 2022
- Full Text
- View/download PDF
41. Utility of bronchoalveolar lavage for COVID-19: a perspective from the Dragon consortium.
- Author
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Tomassetti, Sara, primary, Ciani, Luca, additional, Luzzi, Valentina, additional, Gori, Leonardo, additional, Giuntoli, Leonardo, additional, Lavorini, Federico, additional, Poletti, Venerino, additional, Ravaglia, Claudia, additional, Torrego, Alfons, additional, Maldonado, Fabien, additional, Lentz, Robert, additional, Annunziato, Francesco, additional, Maggi, Laura, additional, Rossolini, Gian Maria, additional, Pollini, Simona, additional, Para, Ombretta, additional, Ciurleo, Greta, additional, Casini, Alessandro, additional, Rasero, Laura, additional, Bartoloni, Alessandro, additional, Spinicci, Michele, additional, Munavvar, Mohammed, additional, Gasparini, Stefano, additional, Comin, Camilla, additional, Cerinic, Marco Matucci, additional, Peired, Anna, additional, Henket, Monique, additional, Benoit, Ernst, additional, Louis, Renaud, additional, Corhay, Jean-Louis, additional, Nardi, Cosimo, additional, and Guiot, Julien, additional
- Published
- 2022
- Full Text
- View/download PDF
42. Differentiation of crescent-forming kidney progenitor cells into podocytes attenuates severe glomerulonephritis in mice
- Author
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Melica, Maria Elena, primary, Antonelli, Giulia, additional, Semeraro, Roberto, additional, Angelotti, Maria Lucia, additional, Lugli, Gianmarco, additional, Landini, Samuela, additional, Ravaglia, Fiammetta, additional, Regina, Gilda La, additional, Conte, Carolina, additional, De Chiara, Letizia, additional, Peired, Anna Julie, additional, Mazzinghi, Benedetta, additional, Donati, Marta, additional, Molli, Alice, additional, Steiger, Stefanie, additional, Magi, Alberto, additional, Bartalucci, Niccolò, additional, Raglianti, Valentina, additional, Guzzi, Francesco, additional, Maggi, Laura, additional, Annunziato, Francesco, additional, Burger, Alexa, additional, Lazzeri, Elena, additional, Anders, Hans-Joachim, additional, Lasagni, Laura, additional, and Romagnani, Paola, additional
- Published
- 2022
- Full Text
- View/download PDF
43. The Antiviral Cytokines IFN-α and IFN-β Modulate Parietal Epithelial Cells and Promote Podocyte Loss: Implications for IFN Toxicity, Viral Glomerulonephritis, and Glomerular Regeneration
- Author
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Migliorini, Adriana, Angelotti, Maria L., Mulay, Shrikant R., Kulkarni, Onkar O., Demleitner, Jana, Dietrich, Alexander, Sagrinati, Costanza, Ballerini, Lara, Peired, Anna, Shankland, Stuart J., Liapis, Helen, Romagnani, Paola, and Anders, Hans-Joachim
- Published
- 2013
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44. FC071: An HDAC Inhibitor Attenuates Crescentic Glomerulonephritis and Avoid Chronic Kidney Disease Enhancing Podocyte Progenitor Differentiation
- Author
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Maria Elena Melica, Giulia Antonelli, Roberto Semeraro, Maria Lucia Angelotti, Letizia De Chiara, Carolina Conte, Gianmarco Lugli, Anna Julie Peired, Gilda La Regina, Benedetta Mazzinghi, Elena Lazzeri, Laura Lasagni, and Paola Romagnani
- Subjects
Transplantation ,Nephrology - Abstract
BACKGROUND AND AIMS Crescentic glomerulonephritis (crescentic GN) encompasses a group of diverse disorders characterized by the presence of massive hyperplasia of parietal epithelial cells (PEC) as the main histopathological lesion at kidney biopsy. It is associated with a rapid decline in kidney function. Typically, crescent formation is the consequence of diverse upstream pathomechanisms involving the specific activation of PEC. PEC normally reside peacefully along Bowman capsule and represent in part renal progenitor cells (RPC). Previous studies observed RPC markers in crescents from patients with different types of glomerulonephritis. Similarities between stem cell niches of bone marrow and kidney, prompted us to hypothesized that crescents result from monoclonal expansion of a single RPC clone conceptually similar to monoclonal diseases originating from hematopoietic stem cells. According to this analogy, we further hypothesized that drugs known to cure monoclonal disease of the hematopoietic stem cells by enforcing their terminal differentiation could also attenuate crescentic glomerulonephritis. METHOD To address this hypothesis, we established a crescentic GN disease model in a conditional transgenic mouse based on the mT/mG and the Confetti reporter that allows lineage tracing and clonal analysis of RPCs. Animals were treated with known pharmacological inhibitors of clonal stem cell proliferation in myeloproliferative disorders. Crescentic lesions were characterized by super-resolution STED microscopy. Finally, we employed single cell RNA sequencing of human renal progenitor cultures to identify the immature progenitor subset-generating crescent in human to identify putative new biomarkers of crescentic GN to validate in biopsy of patients. RESULTS We observed that the crescentic lesions originated from the clonal expansion of single RPC, thus suggesting a clonal stem cell disorder. Therefore, we administrated a series of drugs known to ameliorates myeloproliferative neoplasms to our crescentic GN mouse model as potential therapeutic agents. Treatment with one of the compounds induced a reduction in both proteinuria and crescent formation. STED super-resolution imaging of glomeruli showed that this compound turned the uncontrolled hyperplasia of a specific immature PEC subset into a controlled differentiation into podocytes thereby restoring the injured glomerular filtration barrier. Moreover, delayed drug administration still induced proteinuria remission and avoided long-term development of chronic kidney disease (CKD), an effect associated to a continued generation of new podocytes and crescent regression over time. Single cell RNA sequencing of human RPC identified a new marker of the crescent-generating progenitor cells. Expression of this marker in biopsies of patients with crescentic GN associated with progression toward end stage kidney disease. Treatment of human PEC with the drug that in in vivo experiments showed a therapeutic effect on crescentic GN reduced proliferation of the immature progenitor subset promoting their differentiation into podocytes. CONCLUSION These results demonstrate that glomerular hyperplastic lesions derive from clonal amplification of a RPC subset and that shifting proliferation to podocyte differentiation reverses improves clinical outcome and avoid CKD.
- Published
- 2022
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45. Clinical‐Grade Isolated Human Kidney Perivascular Stromal Cells as an Organotypic Cell Source for Kidney Regenerative Medicine
- Author
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Leuning, Daniëlle G., Reinders, Marlies E.J., Li, Joan, Peired, Anna J., Lievers, Ellen, de Boer, Hetty C., Fibbe, Willem E., Romagnani, Paola, van Kooten, Cees, Little, Melissa H., Engelse, Marten A., and Rabelink, Ton J.
- Published
- 2017
- Full Text
- View/download PDF
46. Evidence for Renal Progenitors in the Human Kidney
- Author
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Lasagni, Laura, primary, Lazzeri, Elena, additional, Peired, Anna, additional, and Romagnani, Paola, additional
- Published
- 2016
- Full Text
- View/download PDF
47. Contributors
- Author
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Al-Awqati, Qais, primary, Arts, H.H., additional, Atala, Anthony, additional, Barnes, Felicity J., additional, Benigni, Ariela, additional, Bertram, John F., additional, Black, M. Jane, additional, Bonventre, Joseph V., additional, Buffington, Deborah A., additional, Bush, Kevin T., additional, Cao, Qi, additional, Carroll, Thomas, additional, Cosgrove, Melanie, additional, Costantini, Frank, additional, Cullen-McEwen, Luise, additional, Davidson, Alan J., additional, Dekel, Benjamin, additional, Dodd, Rachel C., additional, Dressler, Gregory R., additional, Duffield, Jeremy S., additional, Dziedzic, Klaudyna, additional, Ferenbach, David A., additional, Finkelstein, Julia B., additional, Goodyer, Paul, additional, Guay-Woodford, L.M., additional, Hammerman, Marc R., additional, Harris, David C.H., additional, Hiatt, Michael J., additional, Hoy, Wendy E., additional, Hughson, Michael D., additional, Huling, Jennifer C., additional, Humes, H. David, additional, Humphreys, Benjamin D., additional, Ilagan, Roger, additional, Knoers, Nine V.A.M., additional, Kopan, Raphael, additional, Kreidberg, Jordan A., additional, Kwartler, Callie S., additional, Lasagni, Laura, additional, Lazzeri, Elena, additional, Little, Melissa H., additional, Lu, Weining, additional, Macconi, Daniela, additional, Matsell, Douglas G., additional, McMahon, Andrew P., additional, Mendelsohn, Cathy, additional, Moeller, Marcus J., additional, Moritz, Karen M., additional, Nigam, Sanjay K., additional, Nishinakamura, Ryuichi, additional, O’Connor, A.K., additional, Oliver, Juan A., additional, Osafune, Kenji, additional, Oxburgh, Leif, additional, Park, Joo-Seop, additional, Peired, Anna, additional, Pino, Christopher J., additional, Pleniceanu, Oren, additional, Presnell, Sharon, additional, Puelles, Victor G., additional, Quaggin, Susan E., additional, Rabelink, Ton J., additional, Ranghini, Egon, additional, Rapoport, Scott, additional, Reinders, Marlies E.J., additional, Remuzzi, Giuseppe, additional, Ricardo, Sharon D., additional, Romagnani, Paola, additional, Scott, Rizaldy P., additional, Sequeira Lopez, Maria Luisa S., additional, Shepherd, Benjamin, additional, Short, Kieran M., additional, Smyth, Ian M., additional, Susztak, Katalin, additional, Sutherland, Megan R., additional, Taguchi, Atsuhiro, additional, Wang, Yiping, additional, Weber, Stefanie, additional, Westover, Angela J., additional, Yokoo, Takashi, additional, Yoo, James J., additional, and Yu, Jing, additional
- Published
- 2016
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48. Renal Cancer Stem Cells: Characterization and Targeted Therapies
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Anna Julie Peired, Alessandro Sisti, and Paola Romagnani
- Subjects
Internal medicine ,RC31-1245 - Abstract
Renal cell carcinoma (RCC) is a major neoplasm with high incidence in western countries. Tumors are heterogeneous and are composed of differentiated cancer cells, stromal cells, and cancer stem cells (CSCs). CSCs possess two main properties: self-renewal and proliferation. Additionally, they can generate new tumors once transplanted into immunodeficient mice. Several approaches have been described to identify them, through the expression of cell markers, functional assays, or a combination of both. As CSCs are involved in the resistance mechanisms to radio- and chemotherapies, several new strategies have been proposed to directly target CSCs in RCC. One approach drives CSCs to differentiate into cancer cells sensitive to conventional treatments, while the other proposes to eradicate them selectively. A series of innovative therapies aiming at eliminating CSCs have been designed to treat other types of cancer and have not been experimented with on RCC yet, but they reveal themselves to be promising. In conclusion, CSCs are an important player in carcinogenesis and represent a valid target for therapy in RCC patients.
- Published
- 2016
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49. Mesenchymal Stem Cell-Based Therapy for Kidney Disease: A Review of Clinical Evidence
- Author
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Anna Julie Peired, Alessandro Sisti, and Paola Romagnani
- Subjects
Internal medicine ,RC31-1245 - Abstract
Mesenchymal stem cells form a population of self-renewing, multipotent cells that can be isolated from several tissues. Multiple preclinical studies have demonstrated that the administration of exogenous MSC could prevent renal injury and could promote renal recovery through a series of complex mechanisms, in particular via immunomodulation of the immune system and release of paracrine factors and microvesicles. Due to their therapeutic potentials, MSC are being evaluated as a possible player in treatment of human kidney disease, and an increasing number of clinical trials to assess the safety, feasibility, and efficacy of MSC-based therapy in various kidney diseases have been proposed. In the present review, we will summarize the current knowledge on MSC infusion to treat acute kidney injury, chronic kidney disease, diabetic nephropathy, focal segmental glomerulosclerosis, systemic lupus erythematosus, and kidney transplantation. The data obtained from these clinical trials will provide further insight into safety, feasibility, and efficacy of MSC-based therapy in renal pathologies and allow the design of consensus protocol for clinical purpose.
- Published
- 2016
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50. SGLT2 inhibition requires reconsideration of fundamental paradigms in chronic kidney disease, ‘diabetic nephropathy’, IgA nephropathy and podocytopathies with FSGS lesions
- Author
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Anna Julie Peired, Paola Romagnani, and Hans-Joachim Anders
- Subjects
Nephrology ,medicine.medical_specialty ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,Nephropathy ,Diabetic nephropathy ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Focal segmental glomerulosclerosis ,Sodium-Glucose Transporter 2 ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Diabetic Nephropathies ,030212 general & internal medicine ,Renal Insufficiency, Chronic ,Dapagliflozin ,Sodium-Glucose Transporter 2 Inhibitors ,Aged ,Transplantation ,Proteinuria ,Glomerulosclerosis, Focal Segmental ,business.industry ,Glomerulonephritis, IGA ,medicine.disease ,female genital diseases and pregnancy complications ,IgA nephropathy ,chronic kidney disease ,dialysis ,fibrosis ,hyperfiltration ,Diabetes Mellitus, Type 2 ,chemistry ,medicine.symptom ,business ,Kidney disease - Abstract
In 2020, the Dapagliflozin and Prevention of Adverse Outcomes in Chronic Kidney Disease (DAPA-CKD) trial first demonstrated that inhibition of the sodium–glucose transporter-2 (SGLT2) with dapagliflozin attenuates the progression of chronic kidney disease (CKD) with proteinuria in patients with or without diabetes at an unprecedented effect size. These results have far-reaching implications for a series of traditional concepts in Nephrology. It now became obvious that CKD with and without diabetes involves a predominant SGLT2-driven pathophysiology compared with the other pathogenic pathways currently under consideration. As SGLT2 inhibition is similarly efficacious in diabetic and non-diabetic CKD with proteinuria, treating CKD rather than ‘diabetic nephropathy’ becomes the central paradigm. Indeed, in older adults with type 2 diabetes, CKD is rather of multifactorial origin. As the DAPA-CKD trial included more patients with immunoglobulin A nephropathy (IgAN) than any of the previous IgAN trials, dual renin-angiotensin/SGLT2 inhibition may become the new standard. The same applies for patients with podocytopathy-related focal segmental glomerulosclerosis lesions. From now on, IgAN and podocytopathy trials without SGLT2 inhibition as background therapy and without glomerular filtration rate decline as primary outcome criterion will be of limited value. These and other potential implications will trigger broad discussions and secondary research activities with conclusions difficult to predict today. However, one is for sure: Nephrology after the DAPA-CKD trial will be not the same as it was before. Finally!
- Published
- 2020
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