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Molecular analysis in plants requires the use of high quality DNA. Currently, there are a myriad of DNA extraction protocols, which still seems that their applicability across different species is not straightforward. The aim of the present study was to evaluate four different cetyl trimethylammonium bromide (CTAB)-based DNA extraction protocols for Heliconia species from lyophilized fully mature leaves. A split-plot design with two levels (six different species and four DNA extraction methods) was used, where treatments were distributed in a randomized block design with four replications. The response variables were total yield (µg) and purity (A260/A280 nm), and were determined by UV-spectrophotometry and agarose gel electrophoresis, respectively. Additionally, EcoRI enzyme restriction digestion and amplification of the Hc_D6 locus marker were used to evaluate the functionality of the extracted DNA. Analysis of variance (ANOVA) results indicated that the extraction methods had a significant effect on the DNA yield (F = 8.51, df = 3, P

High‐throughput imaging with single‐cell resolution has enabled remarkable discoveries in cell physiology and Systems Biology investigations. A common, and often the most challenging step in all such imaging implementations, is the ability to segment multiple images to regions that correspond to individual cells. Here, a robust segmentation strategy for microbial cells using Quantitative Phase Imaging is reported. The proposed method enables a greater than 99% yeast cell segmentation success rate, without any computationally‐intensive, post‐acquisition processing. We also detail how the method can be expanded to bacterial cell segmentation with 98% success rates with substantially reduced processing requirements in comparison to existing methods. We attribute this improved performance to the remarkably uniform background, elimination of cell‐to‐cell and intracellular optical artifacts, and enhanced signal‐to‐background ratio—all innate properties of imaging in the optical‐phase domain. © 2017 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

Introducción: El objetivo de este trabajo es describir la epidemiología de la infección por virus del papiloma humano (VPH) en un grupo de mujeres privadas de su libertad en el estado de Yucatán, México. Material y método: Se realizó un estudio de corte transversal en el cual se incluyeron a 82 mujeres. Se recolectaron datos epidemiológicos a través de una entrevista directa utilizando un cuestionario estructurado y se tomó muestras de células cervicales. La infección por VPH y los genotipos fueron identificados por técnicas de biología molecular. Resultados: La prevalencia global de VPH fue de 20,7%. Quince diferentes genotipos se identificaron; 60% de bajo riesgo, 26,7% de alto riesgo 13,3% no están clasificados en ninguno de los dos grupos. El genotipo VPH 6/11 fue el más común. El 23,5% (04/17) de las muestras positivas tuvieron infecciones múltiples, tres con dos genotipos, y una con tres. Se encontró asociación entre la infección por VPH y tabaquismo p= 0.0258, OR 3.79 IC 95%(1.01-15.58).Introduction: The aim of this work is describe the epidemiology of HVP amongst female inmates. Material and methods: A total of 82 women were studied in a cross sectional study. Epidemiological data were collected through a direct interview. Samples of cervical cells were taken. HPV and genotypes were identified by molecular test. Results: Global HPV prevalence was 20.7%. Fifteen different genotypes were identified 60% low risk HPV, 26.7% high risk HPV and 13.3% were not classified in any of the two groups. Types 6/11were the most common. 23.5% (04/17) of HPV positives samples had multiple infections, 3 with 2 genotypes and one with 3. Association between infection with HPV and smoking was found, p= 0.0258, OR 3.79 IC 95% (1.01-15.58).

The aim of this work is describe the epidemiology of HVP amongst female inmates.A total of 82 women were studied in a cross sectional study. Epidemiological data were collected through a direct interview. Samples of cervical cells were taken. HPV and genotypes were identified by molecular test.Global HPV prevalence was 20.7%. Fifteen different genotypes were identified 60% low risk HPV, 26.7% high risk HPV and 13.3% were not classified in any of the two groups. Types 6/11 were the most common. 23.5% (04/17) of HPV positives samples had multiple infections, 3 with 2 genotypes and one with 3. Association between infection with HPV and smoking was found, p= 0.0258, OR 3.79 IC 95% (1.01-15.58).