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Papuan orchid species have triggered orchid enthusiasts to be hunted for many purposes such as ornamental plants, medicinal plants and even to breeding program. This intention has encouraged locals who have the resources to trade orchids and extracted from their natural habitat without taking into consideration on how to sustain this resources for the future. Efforts should be conducted in order to enlighten the community not only to preserve this resources but also to encourage locals on how to gain benefit economically using orchids as income generate to support their daily life. Adding to this attempts, extension program such as using materials to create media to grow orchids from coconut waste and sago disposal as basic materials. We employed extension program, focus group discussion, short training course and practicum during the implementation of the activities in each location. Six sites were chosen where the orchid growers and nurseries run by locals and communities living in reserve and non-reserve areas. Five sites located in Jayapura Regency, Jayapura City, Keerom Regency, Biak Regency of Papua Province and Manokwari Regency at West Papua Province. Materials to create media to grow orchids comes from coconut waste and sago disposal as basic materials. Wires and metal screen were used to make frame for potting. We also use tools such as clipper to cut wire. All participants were actively showing their enthusiast to gain knowledge during the course. Some have ask about species name whereas others addressing question of how to reproduce the orchids as well as how to maintain the orchids using home made pot.Keywords: Extension program; community empowerment; organic waste; orchids

A tree may receive compression and flexure combination, and the structural analysis governed by the building code may be capable of estimating the tree’s safety in the built environment. This study proposed to refer to the building code to check the tree dimension adequacy resisting the load. This study simplified the case by focusing only on the self-weight and ignoring the external loads; therefore, the buckling analysis of a slender tapered round column subjected to compression is advocated. Buckling occurs when the tree’s structure can no longer maintain its original shape. Euler and Ylinen’s buckling stress analysis (Method 1) calculated tree safety with a 95% confidence level. This study also applied the Greenhill formula (Method 2) to determine the critical height of a tree receiving the stem weight, then modified it to include the crown weight (Method 3). The three methods calculated the critical height to determine the safety factor (Sf), that is, the ratio of the actual tree height (H) to the 95% confidence level estimated critical height (Hcr). The safety factors were then categorized as unsafe (Sf < 1.00), safe (1.00 < Sf < 1.645), and very safe (1.645 < Sf). This study demonstrated that Method 1 is the most reliable and applicable among other methods. Method 1 resulted in no unsafe trees, 10 safe trees, and 13 very safe trees among the observed excurrent agathis (Agathis dammara). Meanwhile, among the decurrent rain trees (Samanea saman (Jacq.) Merr), 5, 31, and 14 were unsafe, safe, and very safe, respectively.

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

Hartoyo APP, Khairunnisa S, Pamoengkas P, Solikhin A, Supriyanto, Siregar IZ, Prasetyo LB, Istomo. 2022. Estimating carbon stocks of three traditional agroforestry systems and their relationships with tree diversity and stand density. Biodiversitas 23: 6137-6146. One of the important sectors as a strategy for climate resilience and low carbon development in Indonesia is Forestry and Other Land Uses (FOLU) including the agroforestry sector. Carbon stock and tree diversity in an ecosystem are dynamic over time and still need to be investigated. This study was aimed: i) to estimate aboveground carbon stocks in the traditional agroforestry of Mount Halimun Salak National Park (MHSNP), in Kampung Birang and Kampung Merabu, East Kalimantan, as well as ii) to analyze the correlation between carbon stock with tree diversity and stand density in traditional agroforestry of Mount Halimun Salak National Park (MHSNP), in Kampung Birang and Kampung Merabu, East Kalimantan. The total plots were 75 plots (a plot size of 50x50 m), and carbon stock estimation was focused on pole and tree levels. The average carbon stock in the traditional zone of MHSNP, Kampung Birang, and Kampung Merabu respectively was 51.02 Mg C/ha, 96.25 Mg C/ha, and 82.17 Mg C/ha. The carbon stock in traditional agroforestry of all study sites was very significantly affected by the basal area (m2/ha) (p-val

The successful isolation of DNA (Deoxyribonucleic Acid) is essential for the investigation process of forestry molecular genetics. Samples used are usually retrieved either from soft or juvenile plant organs because of their excellent DNA source. However, in certain cases, aforesaid samples are hard to obtain, as for forensic purposes. Alternatively, woods possess potential as alternative source of DNA whose extraction method has been developed with varying degrees of success. However, to date, effectiveness on tropical wood grown in Indonesia has not been widely reported. Therefore, objective of this study was to compare the results of DNA isolation of various dried and fresh wood samples by using two isolation methods: Cetyl Trimethyl Ammonium Bromide (CTAB) and Qiagen DNeasy Plant Mini Kit (QDPMK). Extraction results were visualized in agarose gels and quantified using Nanophotometer NP80 Implen which were then amplified using two universal primers: ITS and rbcL for detecting DNA signals. Extraction results from dried wood indicated no visualization in the gel, while fresh wood samples showed thick smeared bands on both extraction methods. Quantity test results denoted higher concentration in CTAB-extracted samples compared to samples extracted using QDPMK, in both types of samples, even though both resulted in optical density ratios outside the range of purity (λ260/280: 1,8–2,0 and λ260/230: 2,0, respectively). Success rates of ITS and rbcL primary amplification in dried wood samples were quite low yet outputs from the two methods did not differ significantly. Meanwhile, outcome of ITS and rbcL amplification on fresh wood samples had a fairly high success.

The pin-penetration device is a minimally destructive instrument that is widely used to estimate the physical properties of wood, e.g., density, with advantages such as reduced testing times, low costs, and fewer defects induced in the wood being tested. In this study, such a device was used on 25 Indonesian hardwood species with a strength class (SC) distribution from SC II to IV (according to the Indonesian classification of wood SCs). Tests were conducted on three different orthogonal planes, i.e., cross-sectional, radial, and tangential planes. The wood density ranged from 0.28 to 0.88 g/cm3, and the specific gravity was 0.25 to 0.76. The cross-sectional plane penetration depth of the wood was significantly greater than that of either the radial or tangential plane, whereas the pin penetration values of the radial and tangential planes were not significantly different. A prediction model for predicting the density and specific gravity of wood via pin penetration showed a significant regression. Thus, the use of a pin-penetration device was found to be suitable for estimating wood density and specific gravity in a range of SCs of tropical wood species regardless of species.

Tree diversity in Asia's tropical and subtropical forests is central to nature-based solutions. Species vulnerability to multiple threats, which affect provision of ecosystem services, is poorly understood. We conducted a region-wide, spatially explicit assessment of the vulnerability of 63 socioeconomically important tree species to overexploitation, fire, overgrazing, habitat conversion, and climate change. Trees were selected for assessment from national priority lists, and selections were validated by an expert network representing 20 countries. We used Maxent suitability modeling to predict species distribution ranges, freely accessible spatial data sets to map threat exposures, and functional traits to estimate threat sensitivities. Species-specific vulnerability maps were created as the product of exposure maps and sensitivity estimates. Based on vulnerability to current threats and climate change, we identified priority areas for conservation and restoration. Overall, 74% of the most important areas for conservation of these trees fell outside protected areas, and all species were severely threatened across an average of 47% of their native ranges. The most imminent threats were overexploitation and habitat conversion; populations were severely threatened by these factors in an average of 24% and 16% of their ranges, respectively. Our model predicted limited overall climate change impacts, although some study species were likely to lose over 15% of their habitat by 2050 due to climate change. We pinpointed specific natural areas in Borneo rain forests as hotspots for in situ conservation of forest genetic resources, more than 82% of which fell outside designated protected areas. We also identified degraded areas in Western Ghats, Indochina dry forests, and Sumatran rain forests as hotspots for restoration, where planting or assisted natural regeneration will help conserve these species, and croplands in southern India and Thailand as potentially important agroforestry options. Our results highlight the need for regionally coordinated action for effective conservation and restoration.Especies de Árboles Valoradas y Amenazadas de Asia Tropical y Subtropical Resumen La diversidad de árboles en los bosques tropicales y subtropicales de Asia es un eje central para las soluciones basadas en la naturaleza. La vulnerabilidad de las especies ante las múltiples amenazas, las cuales afectan el suministro de servicios ambientales, es un tema poco comprendido. Realizamos una evaluación regional espacialmente explícita de la vulnerabilidad de 63 especies de árboles de importancia socioeconómica ante la sobreexplotación, incendios, sobrepastoreo, conversión del hábitat y cambio climático. Los árboles se seleccionaron para su evaluación a partir de listas nacionales de prioridades, y las selecciones fueron validadas por una red de expertos de 20 países. Usamos el modelado de idoneidad Maxent para predecir el rango de distribución de las especies, conjuntos de datos espaciales de libre acceso para mapear la exposición a las amenazas y rasgos funcionales para estimar la susceptibilidad a las amenazas. Con base en la vulnerabilidad a las amenazas actuales y al cambio climático, identificamos las áreas prioritarias para su conservación y restauración. En general, el 74% de las áreas más importantes para la conservación de estos árboles quedó fuera de las áreas protegidas y todas las especies estaban seriamente amenazadas en promedio en el 47% de su distribución nativa. Las amenazas más inminentes fueron la sobreexplotación y la conversión del hábitat; las poblaciones estuvieron seriamente amenazadas por estos factores en promedio en el 24% y 16% de su distribución, respectivamente. Nuestro modelo predijo un impacto general limitado del cambio climático, aunque algunas especies estudiadas tuvieron la probabilidad de perder más del 15% de su hábitat para el 2050 debido a este factor. Identificamos áreas naturales específicas en las selvas de Borneo como puntos calientes para la conservación in situ de los recursos genéticos forestales, más del 82% de los cuales estaban fuera de las áreas protegidas designadas. También identificamos áreas degradadas en los Ghats Occidentales, los bosques secos de Indochina y las selvas de Sumatra como puntos calientes para la restauración, en donde la siembra o la regeneración natural asistida ayudarán a conservar estas especies. Además, identificamos campos de cultivo al sur de India y Tailandia como potenciales opciones importantes de agrosilvicultura. Nuestros resultados resaltan la necesidad de acciones regionales coordinadas para la conservación y restauración efectivas.【摘要】保护亚洲热带和亚热带森林的树木多样性是基于自然的解决方案的关注重点。物种面对多种威胁的脆弱性会影响生态系统服务的供给, 但目前人们对此仍知之甚少。我们对63种具有社会经济意义的树种面对过度开发、火灾、过度放牧、栖息地转换和气候变化的脆弱性进行了全区域空间显式评估。我们进行评估的树种来自国家优先保护名录, 树种的选择得到代表 20个国家的专家网络的认可。研究使用Maxent适宜性模型预测了物种分布范围, 并用可获取的空间数据集绘制了威胁暴露情况, 用功能特征估计了威胁敏感性。我们利用暴露地图和敏感性估计结果得到了物种特异的脆弱性地图, 并根据物种对当前威胁和气候变化的脆弱性, 确定了保护和恢复的优先区域。总的来说, 对这些树种的保护最重要的区域有 74%在保护区之外, 所有树种平均在其原生分布区 47%的范围内受到严重威胁。最紧迫的威胁是过度开发和栖息地转换;树木种群平均在其 24%和16%的范围内受到这些因素的严重威胁。我们的模型预测得到的气候变化整体影响有限, 尽管一些物种到2050年可能会因气候变化而失去超过15%的栖息地。我们发现婆罗洲雨林中特定的自然区域应作为森林遗传资源原地保护的热点地区, 该区域82%以上的面积位于已有保护区之外。我们还确定了西高止山、中南半岛旱林和苏门答腊雨林的退化地区为恢复热点地区, 在这些地区种植树木或辅助自然再生将有助于保护树种, 而印度南部和泰国的耕地则是潜在的重要农林地。我们的结果强调了为有效保护和恢复而采取区域性协调行动的必要性。【翻译: 胡怡思;审校: 聂永刚】.

Sengon (Falcataria moluccana) plantations, particularly in Java, have been facing threats from gall-rust disease caused by the pathogen Uromycladium falcatariae, which has caused considerable economic losses. Other species with morphological similarities with F. moluccana raises the question of whether the infected and non-infected sengon are the same species. This study aimed to confirm the haplotype of infected and non-infected sengon by gall-rust and compare them with a morphologically similar species of Albizia chinensis using the psbA-trnH intergenic spacer region molecular marker on chloroplast DNA. Wet wood samples from 32 infected plants and 32 non-infected plants were collected from Bogor and Ciamis, West Java, for analysis and comparison with A. chinensis sequences from the same gene region. The results showed that the infected and non-infected sengon came from the same haplotype with a genetic distance of 0 and InDel diversity of 0,031. Meanwhile, from 380 parallel aligned sites, there were 27 different sites between F. moluccana and A. chinensis. The genetic distance of the two species was classified as very low at 0.017. The psbA-trnH intergenic spacer region sequences of sengon in this study were the first to be uploaded to GenBank and can be accessed with accession numbers of LC456638.1 to LC456701.1. ABSTRAK Hutan tanaman sengon (Falcataria moluccana), khususnya di Pulau Jawa, pada umumya menghadapi serangan penyakit karat tumor yang disebabkan oleh patogen Uromycladium falcatariae, sehingga menimbulkan kerugian ekonomi yang cukup besar. Adanya spesies lain yang memiliki kemiripan morfologi dengan F. moluccana menimbulkan pertanyaan apakah sengon yang terserang dan tidak terserang karat tumor merupakan jenis yang sama. Penelitian ini bertujuan untuk mengonfirmasi jenis sengon yang terserang dan tidak terserang karat tumor dan membandingkan dengan spesies yang mirip secara morfologi, yaitu Albizia chinensis menggunakan penanda molekuler daerah psbA-trnH intergenic spacer pada DNA kloroplas. Sampel kayu basah dari 32 tanaman terserang dan 32 tanaman yang tidak terserang karat tumor diambil dari Bogor dan Ciamis, Jawa Barat, untuk dianalisis dan dibandingkan dengan sekuens A. chinensis dari wilayah gen yang sama. Hasil analisis menunjukkan bahwa sengon yang terserang dan tidak terserang berasal dari haplotipe yang sama dengan jarak genetik 0 dan keragaman InDel sebesar 0,031. Sementara itu, dari 380 situs yang sejajar, terdapat 27 situs yang berbeda antara F. moluccana dan A. chinensis. Jarak genetik kedua jenis tersebut tergolong sangat rendah yaitu 0,017. Sekuens daerah psbA-trnH intergenic spacer sengon dalam penelitian ini merupakan yang pertama diunggah di GenBank dan dapat diakses dengan nomer aksesi LC456638.1 sampai LC456701.1.

Several factors may compromise plant identifications provided by local informants, affecting the basis for incorporating local ecological knowledge (LEK) in scientific biodiversity assessments. Our study analyzes how site, informants’ background, and tree characteristics affect the consistency of folk identifications and the apparent correspondence between folk and Linnaean taxonomies. Twenty-eight informants performed in situ identifications of randomly selected trees at two sites in Indonesia and Vietnam. At both sites, older informants and informants who actively used trees had higher probabilities of proposing names for the trees. Naming consistency was higher where informants had good access to and daily use of the forest. Trees with particular morphological and anatomical characteristics, useful species, and landmark trees were more likely to be consistently named. Correspondences between folk and Linnaean taxonomies were clearer in the site with higher folk naming consistency. Findings contribute empirical evidence to inform research design and use of LEK in forest monitoring.

Arobaya AYS, Zuhud EAM, Siregar IZ, Irawati. 2022. Short Communication: Diversity and distribution of epiphytic orchid Dendrobium section Spatulata on the host plants in the Cycloop Mountain Nature Reserve of Papua, Indonesia. Biodiversitas 23: 2025-2034. Knowledge of species diversity and distribution is indispensable to assist in formulating a sound conservation strategy. This is truly significant for epiphytic orchids that grow on tree surfaces or phorophyte. The data and information are still limited despite their important role as biological indicators in forest ecosystems. The aim of the study was to carry out an inventory of epiphytic orchids and the phorophytes (host tress) in the Tropical Rain Forest of Cycloop Mountain Nature Reserve. The study took place in four sites located in the regency and city of Jayapura. Exploratory and sampling strip methods of 20x100 square meters were employed during the fieldwork. Orchids were observed and samples were collected based on their distribution in the host plants divided into five zones (Stem zone I and II; Crown zone III to V). The habitat's vegetation profiles were described using computer-aided design (CAD) verse 2018. Plant samples were identified in herbarium Manokwariense and the identified species were incorporated into CAD. Nineteen species of Dendrobium section Spatulata were identified as living as an epiphyte on the 12 phorophytes. These orchids and the host plants were described, determined and analyzed using PATN software. Most orchids are growing abundant in zone II and III. The phorophytes hosting the orchids in the lowland are mainly Syzygium and Intsia, while in the highland are Agathis and Podocarpus. Two types of vegetation profiles were described as savannah woodland in the low land area and coniferous trees vegetation in the highland above 650 m above sea level.

Rain tree (Samanea saman) is a dominant species commonly planted as a shade tree in urban landscapes in the tropics, including in Indonesia. This species is also targeted for routine inspection and monitoring within tree management systems. Management should particularly focus on tree health through assessment of tree growth conditions as well as tree stability. However, baseline information on the relationships of many key characteristics for the rain tree (e.g., growth, morphometrics, and biomechanics) is lacking. Therefore, an investigation was carried out to determine the relationship of morphometric parameters to tree growth, which included use of an acoustic tool to assess the elasticity of the tree stem. Fifty rain trees were selected for data collection and evaluated using stress wave velocity tools and visual morphometrics assessment. The results demonstrated that, in general, the trees had good vigor and stability. Statistical analysis showed a strong relationship between tree growth parameters and selected morphometric characteristics. The use of a nondestructive acoustic-based instrument enabled determining the modulus of elasticity, which provided equivalent results with the elasticity value obtained through a common method for determining tree stability. Only live crown ratio and tree slenderness parameters had a relationship with the dynamic modulus of elasticity. These results offer a means of measurement that is simpler, less expensive, and faster than current methods, although further testing is needed for different tree species and growth sites.

Jakarta has become one of the cities with the highest air pollution levels among emerging countries. In urban areas such as Jakarta, trees on the roadside have the potential for biomonitoring due to significant pollutants sourced from the traffic. The bark provides information about air pollution due to its furrowed surface and collects atmospheric particle matter (PM). Therefore, the structure of the bark and surface pollutants of 20 rain trees (Samanea saman(Jacq.) Merr.) in the four lots at Sudirman Central Business District (SCBD) Jakarta were assessed. The study aimed to evaluate of air condition in the SCBD area based on the analysis of the bark structure and concentrations of important pollutant elements of Pb (plumbum), Cd (cadmium), Cu (cuprum), Cr (chromium), Mn (manganese), and Zn (zinc) on the bark. The results showed the bark thickness ranges from 3-9 mm with an average of 6.13 mm. The cuticula was gray-black in color, the phloem was brownish, and the cambium layer was very thin. Cu, Cr, Mn, and Zn were detected based on the analysis of the elements. However, Cd was not found in all trees in these four lots. Furthermore, Mn is the dominant pollutant in all trees, more than 40 ppm or more than 69% of the total, followed by Cu, Zn, and Cr. Based on the result, pollutant analysis through accumulated matter on the bark have the potential to be used as biomonitoring in urban areas.

Shorea parvifolia (Dipterocarpaceae) is a widely distributed tree species which is important in terms of ecosystem functioning as well as forestry in Southeast Asia. During glacial periods, substantial precipitation decline is believed to have occurred in Southeast Asia, which considerably changed the distribution of the species. Repeated glacial and inter-glacial fluctuations were found to have influenced the genetic structure of the species, which is important to know for conservation and sustainable use. Leaf samples were collected from 18 populations covering most of the natural distribution of this species including the Malay Peninsula, Sumatra, and Borneo Islands. We investigated these samples using sequence data for eight chloroplast DNA (cpDNA) regions and 14 nuclear EST-SSR loci. The nucleotide diversity of cpDNA is higher in Malay Peninsula populations but the genetic diversity of nuclear DNA is higher in Borneo populations. The genetic structure revealed by nuclear DNA clearly separated Borneo populations from the rest, with an FST value of 0.150, while the genetic structure obtained from cpDNA was less pronounced (FST value = 0.136). Tajima’s D and Fu and Li’s D* for cpDNA showed statistical significance only in populations from Borneo. These results suggested that there has been recent population expansion of S. parvifolia in Borneo.

Mangifera casturi Kosterm., a mango plant from Kalimantan Selatan, Indonesia, has limited genetic information, severely limiting the research on its genetic variation and phylogeny. We collected M. casturi’s genomic information using next-generation sequencing, developed microsatellite markers and performed Sanger sequencing for DNA barcoding analysis. These markers were used to confirm parental origin and genetic diversity of M. casturi hybrids. The clean reads of the Kasturi accession were assembled de novo, producing 259 872 scaffolds (N50 = 1 445 bp). Fourteen polymorphic microsatellite markers were developed from 11 040 microsatellite motif-containing sequences. In total, 58 alleles were produced with a mean of 4.14 alleles per locus. Microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers placed Kasturi, Cuban, Pelipisan, Pinari, and Hambawang in one group, with M. indica as the female ancestor. Meanwhile, the phylogenetic tree of ITS markers indicated several Mangifera species as ancestors of M. casturi. Thus, M. casturi very likely originated from the cross-hybridization of multiple ancestors. Furthermore, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. may have generated much genetic variation. The genetic information for M. casturi will be a resource for breeding improvement, and conservation studies.

Shorea is a genus of the Dipterocarpaceae family which shows high economic and ecological values. Currently, market demand for wood is still met from natural forests that are degrading due to over exploitation. The degraded forests are included in the rehabilitation program that require significant number of planting stocks, mainly from seedlings or wildlings. Correct species identification of planting stocks, particularly wildlings, is an important first step in planting activities and it is relevant with Shorea spp planting stocks due to leaf morphology traits. This study was aimed to examine the traits of leaf morphology of four Shorea species for easy field identification. Samples were taken from five trees for each species. Morphological trait identification was conducted by leaf observation and measurement. The results showed that all four species had the same apex shape. Six of the twelve morphological characteristic of the variable character can influence the morphological characteristics of the leaves, namely the roving leaf, leaf area, leaf length, aspect ratio, form factorand perimeter ratio of diameter. S. leprosula is similar to S. parvifolia, while S. ovalis is similar to S. guiso. Shorea leaf character S. parvifolia differentiated from S. leprosula, while Shorea ovalis similar to Shorea guiso. The LP variable (length from the base of the leaf to the widest point of the leaf) is a variable that can be used as aleaf morphological characteristic of the four Shorea which are compared because each one has a different value. S. ovalis has the highest LP value, followed by S. guiso and S. leprosula, while S. parvifolia has the lowest LP value. Keywords: apex shape, identification, leaf morphology traits, Shorea

Indriani F, Siregar U, Matra DD, Siregar IZ. 2019. Ecological aspects and genetic diversity of Shorea balangeran in two forest types of Muara Kendawang Nature Reserve, West Kalimantan, Indonesia. Biodiversitas 20: 482-488. Shorea balangeran (balangeran) belongs to the Dipterocarpaceae family that is distributed naturally in Indonesia with a wide range of ecological conditions including peat swamp and heath forests. Good adaptability of S. balangeran remains unknown concerning molecular (genetic) responses that are important to provide scientific knowledge for supporting forest restoration, especially for peatland restoration efforts. The main objective of this research was to analyze the ecological aspects and genetic diversity of S. balangeran in two types of forests, namely: peat forests (wetland) and heath forests (dry land). Field research was conducted in Muara Kendawangan Nature Reserve, West Kalimantan following standard vegetation analysis by establishing purposively sample plots (50 m x 50 m). Genetic diversity was assessed by RAPD analysis using ten random polymorphic primers. Results showed that both in heath and peat forests, S. balangeran was dominant, but only at pole and tree stages indicating that poor regeneration capacity of S. balangeran. This finding also confirmed the critically endangered status of S. balangeran as listed in the IUCN Red List of Threatened Species. Genetic analysis using RAPD did not show a clustering of S. balangeran between peat forest and heath forest populations, indicating that S. balangeran has good adaptability to grow in both forest types.

Teak (Tectona grandis Linn. f.), one of the most-valued tree species in the world, is slow-growing with a long period until it can be harvested; therefore, ensuring that only high-quality seedlings or clones are selected for planting is critical. The main objective was to determine performance and repeatability of selected clones in terms of growth and survival rates in different micro-environments. A 2-year clonal trial using 41 clones and a local seedling of teak as a control were grown at 4 microsites differing in spacing, soil fertility and alley crops to assess tree height, diameter and survival rate that was evaluated in Purwakarta, West Java, Indonesia using a randomized complete block design with four replicates of each clone at each microsite. Teak growth was influenced by clone (p

Background: Climate plays an important role in the growth process of various plant species, and the ebony species is no exception to this. Ebony (Diospyros celebica Bakh.) is a Sulawesi endemic flora species whose wood is widely used as a material for light to heavy construction and important raw material for Indonesia’s timber industry. Massive forest exploitation threatens preservation of the Diospyros celebica, so conservation is needed to save it from extinction. However, the difficulty in distinguishing Diospyros celebica in the tillering phase caused many seed collection errors, so a system was needed to identify plants correctly. This study aims to extract leaf morphological features, analyze the correlation between leaf morphological features and climate variables, and classify them based on the region where Diospyros celebica grows. Result: The results show that several leaf morphological characteristics were sufficiently correlated with climate variables such as MAT and MAP. In dry weather, the leaves of Diospyros celebica tend to have relatively small leaf sizes, whereas, in wetter weather, the leaves tend to have a much larger size. The classification results could distinguish leaf morphology based on growing regions with an accuracy rate of 94.59%. Conclusions: By obtaining a high level of classification accuracy, it can be interpreted that the size of the leaf morphology of the same species (Diospyros celebica) is different in each region, influenced by climate variables, in this case MAT and MAP.

Nugroho A, Matra DD, Siregar IZ, Haneda NF, Istikorini Y, Rahmawati R, Amin Y, Siregar UJ. 2021. Early growth evaluation and estimation of heritability in a sengon (Falcataria moluccana) progeny testing at Kediri, East Java, Indonesia. Biodiversitas 22: 2728-2736. Sengon (Falcataria moluccana) is widely cultivated as a monoculture plantation in community forests that still poses a high risk of pest and disease attacks. Although plants naturally show signs of resistance, there is still however a lack of understanding on the influence of relative plant resistance on growth due to environmental and genetic factors. This study was aimed to evaluate the early growth of a sengon progeny testing in Kediri, East Java and to estimate the genetic parameters with respect to its resistance and susceptibility to stem borer and rust disease. Progeny test was designed using randomized complete block design (RCBD) with four blocks as replications. Each block consists of 100 families which was grouped into two categories, 50 families for resistant group and 50 families for susceptible group. Observations were made at the age of 0 and 9 months after planting on 9 individuals per plot in each family. Growth characteristics were measured as germination (%), height (m), and diameter (cm). Results indicated that the sengon growth rate from resistant and susceptible plant groups showed a significant difference. Heritability values are classified as moderate, ranging between 0.107 to 0.133 and 0.102 to 0.150 for height and diameter variables, respectively. These information may be used for gradual selection by considering the appropriate intensity.

Background: Mangifera casturi Kosterm. is an endemic local mango fruit from Kalimantan Selatan, Indonesia. The limited genetic information available on this fruit has severely limited the scope of research into its genetic variation and phylogeny. This study aimed to collect genomic information from M. casturi using next-generation sequencing technology and to develop microsatellite markers and perform Sanger sequencing for DNA barcoding analysis.Results: The clean reads of the Kasturi accession of M. casturi were assembled de novo using a Ray assembler, producing 259,872 scaffolds with an N50 value of 1,445 bp. Fourteen polymorphic microsatellite markers were developed from 11,040 sequences containing microsatellite motifs. In total, 55 alleles were produced, and the mean number of alleles per locus was 3.93. Results from the microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers showed that Kasturi, Mawar, Pelipisan, Pinari, and Hambawang belong to one group, with M. indica as the female ancestor. In comparison, the phylogenetic tree of ITS markers indicated several Mangifera species as multiple ancestors of M. casturi. Conclusions: This study strongly suggested that M. casturi originated from the cross-hybridization of multiple ancestors. Further, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. was hypothesized to produce the observed high genetic variation. The genetic information for this fruit is also a resource for the breeding and improvement as well as for conservation studies of this species.

Objectives Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species. This progress is promising for further improvement in order to acquire more genomic data for research purposes. Therefore, the present study was carried out to determine the effectiveness of ONT in generating long-read DNA sequences using DNA isolated from leaves and wood cores of Macassar ebony (Diospyros celebica Bakh.). Data description Long-read sequences data of leaves and wood cores of Macassar ebony were generated by using the MinION device and MinKnow v3.6.5 (ONT). The obtained data, as the first long-read sequence dataset for Macassar ebony, is of great importance to conserve the genetic diversity, understanding the molecular mechanism, and sustainable use of plant genetic resources for downstream applications.

The purpose of this research was to invent types and abundance insects in sorghum plantation in Lalang Village, Rambutan District, Tebing Tinggi from July to August 2021, with an area of 12 m × 12 m. Insects were collected using sweep traps, pit fall traps, light traps and yellow sticky traps. The collected insects were collected and then were identified. Data analysis was carried out by descriptive quantitative with Purposive Random Sampling (PRS), which identified in the Pest labouratory-USU. The results research identified was four insects Orders (Coleoptera, Diptera, Hemiptera and Lepidoptera) with 9 families (Chrysomelidae, Scarabaeidae, Cecidomyiidae, Muscidae, Aphidae, Dephiciodae, Pentatomidae, Noctuidae, Pyraliidae) and 16 species. The highest insect pest population was at the time of the sixth sampling as many as 634 individuals/plot, the predator population was 248 individuals/plot, and the parasitoid population was 118 individuals. Calculation of richness index R1yst=8.49: R1sn=7.65, eveness index (Eyst=0.59; Esn=0.48) and insects diversity Shannon-Wiener index (H’yst=2.56; H’n=2.13). Then the parameter environmental measure of temperature (30.3-36.2°C), humidity (82-90%) were carried out during the sorghum plantation period.