Search

Background Our purpose was to determine if SIRT1 (rs4746720, rs3740051) genotypes have an influence on the development of pituitary adenoma (PA). Methods The study group included 142 patients with pituitary adenoma (PA) and the control group consisted of 826 healthy people. The genotyping of SIRT1 (rs4746720, rs3740051) was carried out using the real-time polymerase chain reaction method. Results Statistically significant results were obtained in the analysis of SIRT1 rs3740051. Significant differences in genotype (G/G, G/A, A/A) distribution were obtained comparing patients with PA without recurrence and PA with recurrence (0, 17.9, 82.1% vs. 6.7, 6.7, 86.7%, respectively, p = 0.022). Also, statistically significant differences were observed when comparing the genotype (G/G, G/A, A/A) distribution in the non-invasive PA group and the invasive PA group (3.4, 25.9, 70.7% vs. 0, 8.3, 91.7%, respectively, p = 0.003), and allele G was less frequently observed in invasive PA, than in non-invasive PA (4.2% vs. 16.4%, p p = 0.007) and each allele A (OR = 0.229; 95% CI:0.091–0.575; p = 0.002) were associated with lower odds of occurring an invasive PA. Conclusions Our study revealed that SIRT1 rs3740051 is associated with PA recurrence and invasiveness. The haplotype containing alleles C-A in rs12778366-rs3740051 was found to be associated with increased odds of PA development as well.

Purpose: Pituitary adenoma (PA) is an intracranial tumor with the reported prevalence rate of 16.7% [1]. The aim of the present study was to examine the possible involvement of HOXA9 mRNA expression in recurrence and invasiveness of PA. Methodology: The study was carried out at the Department of Ophthalmology and Department of Neurosurgery, Lithuanian University of Health Sciences. The restrospective analysis of MRI data was conducted by an experienced radiologist. HOXA9 mRNA values were investigated in 50 PAs and were normalized to the values of β-actin and GAPDH mRNA, which were used as internal controls. Results: We found no expression of HOXA9 in PA tumors, while the reference genes of β-actin and GAPDH were successfully expressed, indicating that the cDNA synthesis was successful. Conclusions. Our study suggests that HOXA9 mRNA expression is not associated with PA invasiveness and recurrence.

Pituitary adenomas (PAs) present a lot of clinical signs that include symptoms of excessive hormone secretion by the tumor or hormone deficits by the normal pituitary gland. All changes related to grow the tumor mass and compress of surrounding structures: the optic chiasm, cranial nerves [1]. The present study was performed to determine the association between sirtuin 1 (SIRT1) gene single nucleotide polymorphism and PA development and recurrence. Materials and methods: The study included 70 patients with PA. The reference group involved 162 healthy subjects. The genotyping of SIRT1 rs3740051 was performed using real-time polymerase chain reaction method. Results: Statistical analysis did not reveal significant differences in genotype (AA, AG, GG) distribution between the PA and control groups: 77.1%, 20.0%, 2.9% vs. 86.4%, 12.4%, 1.2%, respectively, p=0.202. SIRT1 rs3740051 polymorphism analysis in subgroups of PA by recurrence revealed differences in the genotype distribution between patients with recurrent PA and controls: G/G genotype was more frequent in patients with recurrent PA compared to healthy controls (12.5% vs. 1.2%, p=0.015). Binomial logistic regression analysis of the patients with recurrent PA and the control group revealed significant variables in the co-dominant (OR=11.667; 95% CI: 1.507-90.328; p=0.019) and recessive (OR=11.429; 95% CI: 1.494-87.425; p=0.019). Analysis of genotype distribution between patients with non-recurrent PA and control group did not exhibit any significant differences (p=0.158). Conclusion: The SIRT1 rs3740051 polymorphism associated with recurrent PA development.

Pituitary adenoma (PA) is one of the most common abnormalities in the sellar region. Even knowing that PA is a benign monoclonal neoplasm, it can cause serious complications such as ophthalmological, neurological and endocrinological abnormalities.[1,2] The purpose this investigation was to determine if the methylation status of MMP-14 promoter have an association with pituitary adenoma development. Methods: In this study we used 120 tissue samples of pituitary adenoma. The methylation status of MMP-14 promoter was investigated by methylation specific PCR reaction (MS-PGR). For statistical analyses the IBM program “SPSS Statistics 20” was used. Statistical analysis was conducted to investigate the associations between the methylation status and gender of PA patients. Results: It was discovered that promoter methylation of MMP-14 correlate with the male gender (58.8 % vs. 35.7 %, p=0.022) and unmethylated (non-silenced) MMP14 with the female gender (64.3 % vs. 41.7 %, p=0.027). Conclusion: This study reveals that silencing of the MMP-14 gene correlates to patients’ gender.

Background. Pituitary Adenoma (PA) is the most common pituitary neoplasm. It is classified into non-secreting and secreting forms, which are further divided into subgroups based on the type of hormone released into the circulatory system [1]. While the precise mechanism of PA development remains unknown, many genetic factors are being under the investigation. Aim. The aim of the present study was to evaluate, whether the frequency of the TRIB1 rs6987702 gene polymorphism can be associated with the development of pituitary adenoma. Materials and methods. This study enrolled 70 patients with a diagnosis of pituitary adenoma and a reference group comprised of 100 subjects. The genotyping test results of TRIB1 rs6987702 polymorphism were attained using the real-time polymerase chain reaction (RT-PCR) method. Results. The analysis of TRIB1 rs6987702 gene polymorphism affirmed no differences in the genotype distribution between patients with PA and control group subjects. Among 70 patients with PA, the distribution of T/T, T/C and C/C genotypes was 54.3%, 40% and 5.7%, while in a control group 50%, 41% and 9%, respectively (p > 0.05). Conclusion. TRIB1 rs6987702 gene had no predominant effect on the development in patients with pituitary adenoma.

Background Pituitary adenoma (PA) is a benign brain tumor that can cause neurological, endocrinological and ophthalmological aberrations. Till now there is a need to identify factors that can influence the tumor invasiveness and recurrence. The aim of this study was to evaluate the associations between the signal transducer and activator of transcription 3 (STAT3) promoter methylation, mRNA expression and the invasiveness or recurrence of PAs and patient clinical characteristics. Methods Study participants comprised of 102 subjects with a diagnosis of PA: 54 functioning and 48 non-functioning, 58 invasive and 30 non-invasive PAs and 14 relapses. The bisulfite treatment of tumor DNA and methylation-specific polymerase chain reaction (MS-PCR) method was used to determine the STAT3 gene promoter methylation. For the STAT3 mRNA expression, the first-strand cDNA was produced from total RNA by using reverse transcriptase and quantitative real-time PCR (qRT-PCR) was performed. Results In 10.78% (11/102) of PA tissues STAT3 gene promoter was methylated. A gender of male and patient group older than 60 years were significantly associated with reduced STAT3 mRNA expression (Mann-Whitney test, p = 0.025, p = 0.047, respectively). However, no more statistical differences were found between STAT3 promoter methylation, mRNA expression and patient clinical characteristics or PA invasiveness or recurrence. Conclusions Further investigations are needed to clarify the influence of STAT3 gene promoter methylation and mRNA expression changes in PAs.

Background and Objective. The aim of this study was to evaluate associations between visual functions (visual acuity, perimetry, optic nerve disc condition, and color contrast sensitivity) and pituitary adenoma (PA) diameter. Material and Methods. In the study, 20 patients with PA, which was confirmed by computed tomography or magnetic resonance imaging scans, were examined. The patients were divided into 2 groups: those with a PA diameter of ≤1 cm (14 eyes) and with a PA diameter of >1 cm (26 eyes). The control group comprised 40 healthy age- and gender-matched persons (80 eyes). The diameter of PA, visual acuity, and perimetry were analyzed; the F-M 100 hue test for color discrimination was used in patients with PA. Results. Visual acuity was better in the control group as compared with both groups of patients (1.0 vs. 0.90 [SD, 0.50] and 0.64 [SD, 0.21]; P=0.01; respectively). The results of the Farnsworth- Munsell 100 hue test were also better in the control group compared with the patients with PA of ≤1 cm and >1 cm (error score of 80.1 [SD, 53.0] vs. 131.8 [SD, 30.6] and 244.68 [SD, 51. 6], respectively; P=0.011). There was a very strong positive correlation between the error score of the F-M 100 hue test and PA diameter (r=0.905), but the correlation between the error score and visual acuity (r=–0.32), perimetry (r=0.21), and eye fundus changes (r=0.36) and PA diameter was weak. Conclusions. Our results showed that PA can cause the impairments of visual acuity, perimetry, and color contrast sensitivity. The computerized F-M 100 hue test can be one of the methods for an early diagnosis of chiasm damage in patients with PA.