120 results on '"2 hydroxypropyl β cyclodextrin"'
Search Results
2. Inclusion Complex of Gedunin-2-Hydroxypropyl-Β-Cyclodextrin Prepared by Kneading and Freeze-Drying Methods: Synthesis and Structural Characterization
- Author
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Olusegun G. Ademowo, Olajire Aremu Adegoke, Adedibu C. Tella, Olubunmi Atolani, and Mary O. Ologe
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Freeze-drying ,2 hydroxypropyl β cyclodextrin ,Chemistry ,02 engineering and technology ,General Medicine ,Inclusion (mineral) ,010402 general chemistry ,021001 nanoscience & nanotechnology ,0210 nano-technology ,01 natural sciences ,0104 chemical sciences ,Nuclear chemistry - Abstract
The potential application of gedunin, a pharmacologically active limonoid, is limited in medicine because it has poor aqueous solubility. This study was aimed at preparation and characterization of an inclusion complex of gedunin and 2-hydroxypropyl-β-cyclodextrin (HBD) to increase the solubility in aqueous solvents and thus enhance the possibility of pharmaceutical formulation and oral administration of gedunin. Inclusion complex of gedunin isolated from Entandrophragma angolense heartwood with 2-hydroxypropyl-β-cyclodextrin (HBD) was prepared using freeze-drying and kneading methods. The gedunin-2-hydroxypropyl-β-cyclodextrin complex (GCD) was characterized using elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H-NMR) and X-ray diffraction analysis (XRD). Elemental analysis indicated that gedunin and HBD formed 1:1 stoichiometric inclusion complex. Results of FT-IR indicated that gedunin was stabilized in HBD cavity by intra-molecular hydrogen bonds and van der Waals forces. 1H-NMR revealed that the entire gedunin molecule was not trapped into the core of the HBD. Nevertheless, the fraction trapped may be sufficient to enhance the apparent solubility of gedunin. XRD results showed the formation of new solid crystalline phase. The results obtained by different characterization techniques clearly indicated that both kneading and freeze-drying methods led to inclusion complex formation which may enhance oral administration of gedunin.
- Published
- 2021
3. Gelatin scaffolds loaded with asiaticoside/ <scp>2‐hydroxypropyl‐β</scp> ‐cyclodextrin complex for use as wound dressings
- Author
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Piyachat Chuysinuan, Supanna Techasakul, Pitt Supaphol, Chasuda Choipang, Tanawat Buntum, and Orawan Suwantong
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Scaffold ,food.ingredient ,food ,2 hydroxypropyl β cyclodextrin ,Materials science ,Polymers and Plastics ,Wound dressing ,Gelatin ,Nuclear chemistry - Published
- 2020
4. Non-Covalent Bonding Interaction between Primaquine as Guest and 2-(Hydroxypropyl)-β-Cyclodextrin as Host
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Moorthiraman Murugan, Madi Fatiha, Rajaram Rajamohan, and Arumugam Anitha
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Primaquine ,Polymers and Plastics ,010405 organic chemistry ,Chemistry ,Non covalent ,Organic Chemistry ,In vitro cytotoxicity ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Liquid state ,2 hydroxypropyl β cyclodextrin ,Polymer chemistry ,Materials Chemistry ,medicine ,Absorption (chemistry) ,medicine.drug - Abstract
An inclusion complex of Primaquine (PQ) with 2-(Hydroxypropyl)-β-Cyclodextrin (2-HP-β-CD) in liquid state have been investigated by using absorption and fluorescence spectral techniques. From the s...
- Published
- 2020
5. Comparative study on the effect of two drying methods on the guest encapsulation behavior of osmanthus flavor‐2‐hydroxypropyl‐β‐cyclodextrin inclusion complex
- Author
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Liu Qinghe, Du Peiting, Jing Hu, Liu Shanshan, and Deng Weijun
- Subjects
Osmanthus ,2 hydroxypropyl β cyclodextrin ,biology ,Chemistry ,Organic chemistry ,General Chemistry ,biology.organism_classification ,Flavor ,Food Science ,Encapsulation (networking) - Published
- 2020
6. New Enhanced Method for Determination of Trace Sulfamethodxazole Based on the Fluorescence Behaviors of Cyclodextrins in Water Solutions
- Author
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Ping Wang, Xue-jing Si, Tun-hua Wu, and Hong-ling Wang
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Sociology and Political Science ,Correlation coefficient ,Clinical Biochemistry ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Fluorescence spectroscopy ,2 hydroxypropyl β cyclodextrin ,Control sample ,Environment water ,Spectroscopy ,Detection limit ,Cyclodextrins ,Sulfonamides ,Chromatography ,010405 organic chemistry ,Chemistry ,Water ,Fluorescence ,0104 chemical sciences ,Solutions ,Clinical Psychology ,Spectrometry, Fluorescence ,Thermodynamics ,Law ,Water Pollutants, Chemical ,Social Sciences (miscellaneous) - Abstract
The widespread occurrence of sulfonamides (SAs) in the environment water has rasied great concerns about their potential to antibiotics resistance. In this study, the fluorescence behaviors of sulfamethoxazole (SMZ) representing certain properties of the SAs mixed with three different kinds of cyclodextrins (CDs) in water solutions were investigated, respectively. The result reported that the shapes of the fluorescence peak and its position for the SMZ that were mixed with the CDs were almost the same as those of the standard SMZ, respectively. In addition, compared with the identical control sample the fluorescence of SMZ mixed with each of the CD was greatly enhanced. Therefore, a new simple, and sensitive spectrofluorimetric method for the determination of SMZ was established in water solutions. and the dynamic linear ranges varied from 0.01 to 0.7 mg/L with the detection limit of 7.1 ng/L. And the correlation coefficient was more than 99.9%. Significantly, this new method was successfully applied to direct determination of SMZ in pharmaceutical compounds. Moreover, the results showed that the SMZ could separately form the 1:1 supramolecular compound with each of the CD.
- Published
- 2020
7. Antihypertensive activity and molecular interactions of irbesartan in complex with 2‐hydroxypropyl‐β‐cyclodextrin
- Author
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Dimitrios Ntountaniotis, Eirini Christodoulou, Dimitrios Damalas, Georgios Liapakis, Georgia Valsami, Vlasios Karageorgos, Nikolaos Naziris, Nikolaos S. Thomaidis, Thomas Mavromoustakos, Maria V. Chatziathanasiadou, Andreas G. Tzakos, Maria Chountoulesi, Georgios Leonis, and Costas Demetzos
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Spectrometry, Mass, Electrospray Ionization ,Drug Compounding ,Molecular Conformation ,Molecular Dynamics Simulation ,01 natural sciences ,Biochemistry ,Irbesartan ,2 hydroxypropyl β cyclodextrin ,Drug Discovery ,medicine ,Humans ,Beneficial effects ,Antihypertensive Agents ,Pharmacology ,Molecular interactions ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Combinatorial chemistry ,2-Hydroxypropyl-beta-cyclodextrin ,0104 chemical sciences ,Drug Liberation ,010404 medicinal & biomolecular chemistry ,Freeze Drying ,Solubility ,Drug delivery ,Lipophilicity ,Molecular Medicine ,medicine.drug - Abstract
Irbesartan (IRB) exerts beneficial effects either alone or in combination with other drugs on numerous diseases, such as cancer, diabetes, and hypertension. However, due to its high lipophilicity, IRB does not possess the optimum pharmacological efficiency. To circumvent this problem, a drug delivery system with 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) was explored. The 1:1 complex between IRB and 2-HP-β-CD was identified through ESI QTF HRMS. Dissolution studies showed a higher dissolution rate of the lyophilized IRB-2-HP-β-CD complex than the tablet containing IRB at pH = 1.2. DSC results revealed the differences of the thermal properties between the complex and various mixtures consisting of the two components, namely IRB and 2-HP-β-CD. Interestingly, depending on the way the mixture preparation was conducted, different association between the two components was observed. Molecular dynamics (MD) simulations predicted the favorable formation of the above complex and identified the dominant interactions between IRB and 2-HP-β-CD. In vitro pharmacological results verified that the inclusion complex not only preserves the binding affinity of IRB for AT1R receptor, but also it slightly increases it. As the complex formulation lacks the problems of the tablet, our approach is a promising new way to improve the efficiency of IRB.
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- 2020
8. Author response for 'Encapsulation of β‐carotene in 2‐hydroxypropyl‐β‐cyclodextrin/carrageenan/soy protein using a modified spray drying process'
- Author
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Mahalakshmi Lakshmanan, Anandharamakrishnan Chinnaswamy, and Jeyan A. Moses
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chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Chromatography ,chemistry ,medicine.medical_treatment ,Scientific method ,Spray drying ,Carotene ,medicine ,Soy protein ,Carrageenan - Published
- 2021
9. Review for 'Encapsulation of β‐carotene in 2‐hydroxypropyl‐β‐cyclodextrin/carrageenan/soy protein using a modified spray drying process'
- Author
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Yang Yuan
- Subjects
chemistry.chemical_compound ,Chromatography ,2 hydroxypropyl β cyclodextrin ,chemistry ,Scientific method ,medicine.medical_treatment ,Spray drying ,Carotene ,medicine ,Soy protein ,Carrageenan - Published
- 2021
10. Differential Properties of Nanoporous Nanosponges Prepared from β-Cyclodextrin and 2-Hydroxypropyl β-Cyclodextrin
- Author
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Anandam Singireddy, Mohan M. Varma, Sobhitarani Pedireddi, and Vijaya Ratna Jayanthi
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chemistry.chemical_classification ,2 hydroxypropyl β cyclodextrin ,Cyclodextrin ,Chemistry ,Nanoporous ,Nanosponges ,General Medicine ,Combinatorial chemistry - Published
- 2019
11. Antioxidant Activity and Hepatotoxicity of Flavonoids and Their Metal Complexes Through Co‐Administration of β‐Cyclodextrin
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Tahir Ali, Zaman Ashraf, Naveed Kausar Janjua, Erum Jabeen, Safeer Ahmed, Iram Murtaza, Saima Kalsoom, and Nosheen Masood
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chemistry.chemical_classification ,Antioxidant ,Cyclodextrin ,medicine.medical_treatment ,General Chemistry ,Metal ,2 hydroxypropyl β cyclodextrin ,chemistry ,Human plasma ,visual_art ,visual_art.visual_art_medium ,medicine ,Co administration ,Nuclear chemistry - Published
- 2019
12. 2-Hydroxypropyl-β-cyclodextrin-enhanced pharmacokinetics of cabotegravir from a nanofluidic implant for HIV pre-exposure prophylaxis
- Author
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Jesus Paez-Mayorga, Giacomo Bruno, Alessandro Grattoni, Cecilia Martini, Corrine Ying Xuan Chua, Jessica Rhudy, Madhuri Manohar, Nicola Di Trani, Mark A. Marzinke, Fernanda P. Pons-Faudoa, Greta Varchi, Kevin Gwenden, and Antons Sizovs
- Subjects
Male ,Anti-HIV Agents ,Pyridones ,Human immunodeficiency virus (HIV) ,Pharmaceutical Science ,HIV Infections ,02 engineering and technology ,Pharmacology ,medicine.disease_cause ,Article ,Rats, Sprague-Dawley ,03 medical and health sciences ,Pre-exposure prophylaxis ,chemistry.chemical_compound ,Drug Delivery Systems ,Cabotegravir ,2 hydroxypropyl β cyclodextrin ,Pharmacokinetics ,medicine ,Animals ,030304 developmental biology ,0303 health sciences ,business.industry ,fungi ,HIV ,Implant ,food and beverages ,021001 nanoscience & nanotechnology ,PrEP ,2-Hydroxypropyl-beta-cyclodextrin ,Rats ,Integrase strand transfer inhibitor ,Formulation ,chemistry ,Pre-Exposure Prophylaxis ,0210 nano-technology ,business ,Dosing Frequency - Abstract
Preexposure prophylaxis (PrEP) with antiretrovirals (ARV) can prevent human immunodeficiency virus (HIV) transmission, but its efficacy is highly dependent on strict patient adherence to daily dosing regimen. Long-acting (LA) ARV formulations or delivery systems that reduce dosing frequency may increase adherence and thus PrEP efficacy. While cabotegravir (CAB) long-acting injectable (CAB LA), an integrase strand transfer inhibitor (INSTI), reduces dosing frequency to bimonthly injections, variable pharmacokinetics (PK) between patients and various adverse reactions necessitate improvement in delivery methods. Here we developed a subcutaneously implantable nanofluidic device for the sustained delivery of CAB formulated with 2-hydroxypropyl-beta-cyclodextrin (beta CAB) and examined the pharmacokinetics (PK) in Sprague-Dawley rats for 3 months in comparison to CAB. Our study demonstrated beta CAB treatment group maintained clinically-relevant plasma CAB concentrations 2 times above the protein-adjusted concentration that inhibits viral replication by 90% (2xPA-IC90) and drug penetration into tissues relevant to HIV-1 transmission. Further, we successfully fitted plasma CAB concentrations into a PK model (R-2 = 0.9999) and determined CAB apparent elimination half-life of 47 days. Overall, our data shows the potential of sustained release of beta CAB via a nanofluidic implant for long-term PrEP delivery, warranting further investigation for efficacy against HIV infections.
- Published
- 2019
13. Thermodynamic parameters for the complexation of water-soluble betulin derivatives with (2-hydroxypropyl)-β-cyclodextrin determined by affinity capillary electrophoresis
- Author
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Viktoria V. Sursyakova, Anatoly I. Rubaylo, and Vladimir A. Levdansky
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Betulin ,Enthalpy ,02 engineering and technology ,Atmospheric temperature range ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Capillary electrophoresis ,Triterpenoid ,Water soluble ,chemistry ,Computational chemistry ,Materials Chemistry ,Molecule ,Physical and Theoretical Chemistry ,0210 nano-technology ,Spectroscopy - Abstract
The interaction between (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) and water-soluble betulin derivatives, betulin 3,28-disulfate (DSB) and betulin 3-acetate-28-sulfate (ASB), belonging to the class of pentacyclic lupane triterpenoids, was studied using affinity capillary electrophoresis. It was found that 1:1 and 1:2 complexes were formed. The stability constants of the complexes in the temperature range of 293.15–318.15 K were determined. The values obtained are sufficiently large; log K (1:1) and log K (1:2) are 4.25–5.02 and 6.08–7.59, respectively. This phenomenon can be explained by the presence of broad hydrophobic regions in the molecules of the compound studied. The stability constants decrease with increasing temperature. The stability constants for ASB complexes are slightly higher as compared to the constants for DSB complexes. The thermodynamic parameters for the complexation were calculated from the van't Hoff plots. The complexation was found to be controlled by the enthalpy change. The obtained values of stability constants at 298 K were compared with values for the β-CD complexes of the compounds under study and for the HP-β-CD and β-CD complexes of water-insoluble betulin derivatives. It was found that water-soluble betulin derivatives form more stable complexes with CDs as compared to water-insoluble derivatives (betulonic and betulinic acids), and the HP-β-CD complexes are more stable than the β-CD complexes.
- Published
- 2019
14. Investigating the oxyresveratrol β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin complexes: The effects on oxyresveratrol solution, stability, and antibrowning ability on fresh grape juice
- Author
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Chen Hongbin, Zong-Ping Zheng, Jie Chen, Lin Luan, Yong-Qiang Cheng, Jianfei He, and Fengxian Guo
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0106 biological sciences ,chemistry.chemical_classification ,Aqueous solution ,Cyclodextrin ,04 agricultural and veterinary sciences ,040401 food science ,01 natural sciences ,Oxyresveratrol ,chemistry.chemical_compound ,0404 agricultural biotechnology ,2 hydroxypropyl β cyclodextrin ,chemistry ,010608 biotechnology ,Physical stability ,Solubility ,Dissolution ,Food Science ,Nuclear chemistry - Abstract
The inclusion complexes of oxyresveratrol (Oxy) with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) were prepared and characterized by UV–vis spectroscopy, FT-IR spectroscopy, XRD, TG-DTG and SEM. Based on the optimal formulation, 222.23 ± 1.52 μg and 232.65 ± 2.64 μg of Oxy was included in 1.00 mg of the Oxy-β-CD and Oxy-HP-β-CD dried powder with the water solubility up to 14.44 ± 0.83 mg/mL and 47.33 ± 0.78 mg/mL, yielding about 30 and 100-fold increase in Oxy solubility (about 0.47 mg/mL), respectively. The physical stability and dissolution rate study showed that the formation of inclusion complexes significant enhanced the storage stability of Oxy and its dissolution rate. These results indicated that β-CD and HP-β-CD may be suitable delivery systems of Oxy. Furthermore, as illustrated in this study, inclusion complexes exhibited strong antibrowning effects on fresh grape juice in 24 h, showing its great potential application in the food industry.
- Published
- 2019
15. Dual 2-Hydroxypropyl-β-Cyclodextrin and 5,10,15,20-Tetrakis (4-Hydroxyphenyl) Porphyrin System as a Novel Chiral-Achiral Selector Complex for Enantioseparation of Aminoalkanol Derivatives with Anticancer Activity in Capillary Electrophoresis
- Author
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Mariola Napiórkowska and Błażej Grodner
- Subjects
Porphyrins ,capillary electrophoresis ,Pharmaceutical Science ,Antineoplastic Agents ,02 engineering and technology ,Buffers ,01 natural sciences ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,Electrolytes ,chemistry.chemical_compound ,Capillary electrophoresis ,Blood serum ,2 hydroxypropyl β cyclodextrin ,lcsh:Organic chemistry ,Limit of Detection ,Drug Discovery ,anticancer drug ,Amines ,Physical and Theoretical Chemistry ,drug monitoring ,010401 analytical chemistry ,Organic Chemistry ,Phosphate buffered saline ,Temperature ,Electrophoresis, Capillary ,Reproducibility of Results ,Stereoisomerism ,Repeatability ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Porphyrin ,2-Hydroxypropyl-beta-cyclodextrin ,0104 chemical sciences ,chiral selectors ,chemistry ,Chemistry (miscellaneous) ,Regression Analysis ,Molecular Medicine ,Enantiomer ,0210 nano-technology ,Selectivity ,Nuclear chemistry - Abstract
In this study, a complex consisting of 2-hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin, (named dual chiral-achiral selector complex) was used for the determination of two novel potential anticancer agents of (I) and (II) aminoalkanol derivatives. This work aimed at developing an effective method that can be utilized for the determination of I (S), I (R), and II (S) and II (R) enantiomers of (I) and (II) compounds through the use of a dual chiral-achiral selector complex consisting of hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin system by applying capillary electrophoresis. This combination proved to be beneficial in achieving high separation selectivity due to the combined effects of different modes of chiral discrimination. The enantiomers of (I) and (II) compounds were separated within a very short time of 3.6–7.2 min, in pH 2.5 phosphate buffer containing 2-hydroxypropyl-β-cyclodextrin and 5,10,15,20-tetrakis (4-hydroxyphenyl) porphyrin system at a concentration of 5 and 10 mM, respectively, at 25 °C and +10 kV. The detection wavelength of the detector was set at 200 nm. The LOD for I (S), I (R), II (S), and II (R) was 65.2, 65.6, 65.1, and 65.7 ng/mL, respectively. LOQ for I (S), I (R), II (S), and II (R) was 216.5, 217.8, 217.1, and 218.1 ng/mL, respectively. Recovery was 94.9–99.9%. The repeatability and reproducibility of the method based on the values of the migration time, and the area under the peak was 0.3–2.9% RSD. The stability of the method was determined at 0.1–4.9% RSD. The developed method was used in the pilot studies for determining the enantiomers I (S), I (R), II (S), and II (R) in the blood serum.
- Published
- 2021
16. Differential Scanning Calorimetry (DSC) on Sartan/Cyclodextrin Delivery Formulations
- Author
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Costas Demetzos, Nikolaos Naziris, Thomas Mavromoustakos, Dimitrios Ntountaniotis, and Maria Chountoulesi
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chemistry.chemical_classification ,Cyclodextrin ,technology, industry, and agriculture ,02 engineering and technology ,021001 nanoscience & nanotechnology ,030226 pharmacology & pharmacy ,Combinatorial chemistry ,03 medical and health sciences ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Differential scanning calorimetry ,chemistry ,Drug delivery ,Molecule ,0210 nano-technology - Abstract
Differential scanning calorimetry (DSC) is a widely utilized method for the interactions of drug molecules with drug delivery systems (DDSs). Herein is described a protocol for studying the interactions and entrapment efficiency of the prototype sartan losartan and the polydynamic, structurally similar irbesartan inside the nontoxic 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD). The thermal scan properties of both sartan molecules have been studied when physically mixed or complexed with the cyclodextrin. The thermograms indeed showed significant differences between the mixtures and complexes, establishing DSC as a valuable method to characterize the state of the drugs in these pharmaceutical formulations.
- Published
- 2020
17. Study of Candesartan Cilexetil: 2-Hydroxypropyl-β-Cyclodextrin Interactions: A Computational Approach Using Steered Molecular Dynamics Simulations
- Author
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Thomas Mavromoustakos and Sofia Kiriakidi
- Subjects
Drug ,010304 chemical physics ,Chemistry ,media_common.quotation_subject ,010401 analytical chemistry ,technology, industry, and agriculture ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,Candesartan ,Molecular dynamics ,2 hydroxypropyl β cyclodextrin ,0103 physical sciences ,Amphiphile ,Lipophilicity ,medicine ,Umbrella sampling ,Drug metabolism ,media_common ,medicine.drug - Abstract
Cyclodextrins (CDs) are widely used in the pharmaceutical industry as transporters of lipophilic drugs due to their amphiphilic nature. The detailed study of the molecular interactions of drug complexes with CDs is very important in designing the best formulation for the transportation of lipophilic drugs. The drug: CD binding should be strong enough so that the complex is stable in the aquatic environment of the extracellular fluids, but also not very strong in order for the drug to be capable for being released in the proximity of the target receptor. In a recent study, we investigated the ΔG of binding between the commercially available, nontoxic 2-hydroxypropyl-β-cyclodextrin (2-HP-B-CD) and the antihypertensive drug candesartan cilexetil (CC), with the use of steered (or biased) molecular dynamics (sMD) and umbrella sampling method. This chapter describes comprehensively how to perform sMD and umbrella sampling in order to calculate the ΔGbinding of CC to the 2-HP-B-CD.
- Published
- 2020
18. RSM-CCD optimized in air synthesis of novel kappa-carrageenan/tamarind kernel powder hybrid polymer network incorporated with inclusion complex of (2-hydroxypropyl)-β-cyclodextrin and adenosine for controlled drug delivery
- Author
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Rajeev Jindal and Vasudha Vaid
- Subjects
Chromatography ,2 hydroxypropyl β cyclodextrin ,Polymer network ,Kappa-Carrageenan ,Chemistry ,Kernel (statistics) ,Drug delivery ,medicine ,Pharmaceutical Science ,Adenosine ,medicine.drug - Published
- 2022
19. Characterization of an aryl piperazine/2-hydroxypropyl-β-cyclodextrin association, a complex with antidiabetic potential
- Author
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John C. Stephens, Darren S. D. Martin, Gemma K. Kinsella, Robert Devine, and John B. C. Findlay
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Trifluoromethyl ,Aryl ,Diabetes ,2-Hydroxypropyl-β-cyclodextrin ,General Chemistry ,In vitro ,lcsh:Chemistry ,chemistry.chemical_compound ,Piperazine ,Differential scanning calorimetry ,2 hydroxypropyl β cyclodextrin ,Aryl piperazine ,chemistry ,lcsh:QD1-999 ,Stability constants of complexes ,Stoichiometry ,Biochemistry, Biophysics, and Structural Biology ,Nuclear chemistry - Abstract
This study explores the molecular association between 4-(thiophen-2-yl)-1-(4-(4-(trifluoromethyl)phenyl)piperazin-1-yl)butan-1-one (RTC1), an antidiabetic compound recently reported by our research group with challenging aqueous solubility, and 2-hydroxypropyl-β-cyclodextrin (HPBCD). The formation of a RTC1/HPBCD complex resulted in improved solubility. A phase-solubility diagram was used to determine the complex stability constant and stoichiometric ratio. 2D 1H NMR spectroscopy was utilized to study the molecular interaction between RTC1 and HPBCD in the complex. Differential scanning calorimetry and scanning electron microscopy was also employed to confirm complex formation. In vitro biological evaluation, using a glucose uptake assay, showed that the homogeneous RTC1/HPBCD complex solution showed the same activity to that of RTC1 alone, with no reduction in activity due to the presence of HPBCD.
- Published
- 2020
20. The effect of complexation with cyclodextrins on the antioxidant and antimicrobial activity of ellagic acid
- Author
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Emilija Jocic, Mirjana Popsavin, Ivan M. Savic, Srdjan Rakic, Ivana Savic-Gajic, and Vesna Nikolić
- Subjects
Magnetic Resonance Spectroscopy ,Antioxidant ,medicine.medical_treatment ,Pharmaceutical Science ,02 engineering and technology ,030226 pharmacology & pharmacy ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Anti-Infective Agents ,Ellagic Acid ,X-Ray Diffraction ,Candida albicans ,Spectroscopy, Fourier Transform Infrared ,Escherichia coli ,medicine ,Humans ,Organic chemistry ,Cyclodextrins ,Chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,Antimicrobial ,3. Good health ,0210 nano-technology ,Ellagic acid - Abstract
The aim of the paper was to develop the simple procedures for preparation of inclusion complexes of ellagic acid (EA) with cyclodextrins (CDs) and to investigate their antioxidant and antimicrobial activity.The structural characterization was carried out using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and nuclear magnetic resonance (NMR) methods. The phase solubility technique was used to investigate the interactions between 'host' and 'guest' molecules and to estimate the molar ratio between them. The antioxidant and antimicrobial activity of EA and inclusion complexes were determined.The apparent stability constants were found to be 117 dmThe stability constants indicated the rapid release of EA from the inclusion complexes in the aqueous medium at 25 °C. The antioxidant activity of EA was increased, while the antimicrobial activity was preserved after complexation with CDs.
- Published
- 2018
21. A water-based topical Chinese traditional medicine (Zicao) for wound healing developed using 2-hydroxypropyl-β-cyclodextrin
- Author
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Chin Mu Hsu, Song Cu Yu, Ta Chen Chen, Fuu Jen Tsai, and Yuhsin Tsai
- Subjects
Administration, Topical ,Proton Magnetic Resonance Spectroscopy ,medicine.disease_cause ,01 natural sciences ,Mass Spectrometry ,Rats, Sprague-Dawley ,2-Hydroxypropyl-beta-cyclodextrin ,Colloid and Surface Chemistry ,2 hydroxypropyl β cyclodextrin ,In vivo ,0103 physical sciences ,Image Processing, Computer-Assisted ,medicine ,Animals ,Medicine, Chinese Traditional ,Physical and Theoretical Chemistry ,Solubility ,Wound Healing ,010304 chemical physics ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Water ,Surfaces and Interfaces ,General Medicine ,Endocytosis ,Water based ,0104 chemical sciences ,Irritation ,Wound healing ,Chinese traditional medicine ,Chromatography, Liquid ,Drugs, Chinese Herbal ,Biotechnology - Abstract
Zicao is a traditional Chinese herbal medicine that has been used for the topical treatment of wounds in the form of oil-based ointment for several hundred years. To overcome the disadvantages of oil-based ointment such as irritation, discomfort, and difficulty in cleaning, this study developed a water-based topical formulation of Zicao. An ethanol extract of Zicao was included in 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to form a water-soluble Zicao-HP-β-CD complex. The formation of the Zicao-HP-β-CD complex was determined using LC-MS, 1H NMR, ROSEY, and solubility analysis. The bioactivity of Zicao-HP-β-CD complex in aqueous solution was evaluated using cellular uptake in vitro and experimental excision wounds in vivo. The LC-MS, 1H NMR, ROESY, and solubility analyses results show that Zicao extract was successfully included by the HP-β-CD. The results of the cellular uptake in vitro and wound healing in vivo suggest that the effect of Zicao was enhanced following the formation of the Zicao-HP-β-CD complex. Therefore, we concluded that complexation with HP-β-CD might provide a potential method for developing an effective water-based topical solution of Zicao.
- Published
- 2018
22. Predicting the Binding Mode of 2-Hydroxypropyl-β-cyclodextrin to Cholesterol by Means of the MD Simulation and the 3D-RISM-KH Theory
- Author
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Fumio Hirata, Takeshi Kikuchi, Tetsumi Irie, Yuji Hayashino, Masatake Sugita, and Hidetoshi Arima
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0301 basic medicine ,Molecular Conformation ,Economic shortage ,Molecular Dynamics Simulation ,010402 general chemistry ,01 natural sciences ,03 medical and health sciences ,Molecular dynamics ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Glycolipid ,Materials Chemistry ,Humans ,Physical and Theoretical Chemistry ,Binding site ,Cellular compartment ,Binding Sites ,Models, Statistical ,Chemistry ,Cholesterol ,Niemann-Pick Disease, Type C ,Cyclodextrin Derivatives ,2-Hydroxypropyl-beta-cyclodextrin ,0104 chemical sciences ,Surfaces, Coatings and Films ,030104 developmental biology ,Biophysics ,Thermodynamics ,lipids (amino acids, peptides, and proteins) ,Lysosomes ,Dimerization - Abstract
It has been found that a cyclodextrin derivative, 2-hydroxypropyl-β-cyclodextrin (HPβCD), has reasonable therapeutic effect on Niemann-Pick disease type C, which is caused by abnormal accumulation of unesterified cholesterol and glycolipids in the lysosomes and shortage of esterified cholesterol in other cellular compartments. We study the binding affinity and mode of HPβCD with cholesterol to elucidate the possible mechanism of HPβCD for removing cholesterol from the lysosomes. The dominant binding mode of HPβCD with cholesterol is found based on the molecular dynamics simulation and a statistical mechanics theory of liquids, or the three-dimensional reference interaction site model theory with Kovalenko-Hirata closure relation. We examine the two types of complexes between HPβCD and cholesterol, namely, one-to-one (1:1) and two-to-one (2:1). It is predicted that the 1:1 complex makes two or three types of stable binding mode in solution, in which the βCD ring tends to be located at the edge of the steroid skeleton. For the 2:1 complex, there are four different types of the complex conceivable, depending on the orientation between the two HPβCDs: head-to-head (HH), head-to-tail (HT), tail-to-head (TH), and tail-to-tail (TT). The HT and HH cyclodextrin dimers show higher affinity to cholesterol compared to the other dimers and to all the binding modes of 1:1 complexes. The physical reason why the HT and HH dimers have higher affinity compared to the other complexes is discussed based on the consistency with the 1:1 complex. On the one hand, in case of the HT and HH dimers, the position of each CD in the dimer along the cholesterol chain comes right on or close to one of the positions where a single CD makes a stable complex. On the other hand, one of the CD molecules is located on unstable region along the cholesterol chain, for the case of TH and TT dimers.
- Published
- 2018
23. Intracerebroventricular 2-hydroxypropyl-β-cyclodextrin improves not only neurological symptoms but also hepatic abnormalities in Niemann-Pick disease type C model mice and patients
- Author
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Tsuyoshi Shuto, Madoka Fukaura, Hidetoshi Arima, Takafumi Sakakibara, Katsumi Higaki, Tetsumi Irie, Muneaki Matsuo, Shunsuke Kamei, Toru Takeo, Yoshio Sakiyama, Naoki Ushihama, Naomi Nakagata, Hiroshi Katsuki, Keiichi Motoyama, Hirofumi Kai, Takumi Era, Yoichi Ishituka, Taishi Higashi, Yuki Kurauchi, and Yuki Kondo
- Subjects
medicine.medical_specialty ,Niemann–Pick disease, type C ,Endocrinology ,2 hydroxypropyl β cyclodextrin ,Chemistry ,Applied Mathematics ,General Mathematics ,Internal medicine ,medicine ,medicine.disease - Published
- 2018
24. Ultrasound-mediated molecular self-assemble of thymol with 2-hydroxypropyl-β-cyclodextrin for fruit preservation
- Author
-
Chongde Sun, Jue Wu, Cui Sun, Yue Wang, He Zhang, Jinping Cao, Lingxia Huang, and Jiebiao Chen
- Subjects
01 natural sciences ,Alternaria alternata ,Analytical Chemistry ,chemistry.chemical_compound ,0404 agricultural biotechnology ,2 hydroxypropyl β cyclodextrin ,X-Ray Diffraction ,In vivo ,Spectroscopy, Fourier Transform Infrared ,Thermal stability ,Solubility ,Thymol ,Botrytis cinerea ,Cyclodextrins ,Penicillium digitatum ,Calorimetry, Differential Scanning ,biology ,010401 analytical chemistry ,Penicillium ,Alternaria ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,040401 food science ,2-Hydroxypropyl-beta-cyclodextrin ,0104 chemical sciences ,chemistry ,Fruit ,Botrytis ,Food Science ,Nuclear chemistry - Abstract
In this study, the inclusion complex (IC) of thymol with 2-hydroxypropyl-β-cyclodextrin (HPβCD) was fast synthetized by ultrasonic technology and its antifungal activities were evaluated. The thymol/HPβCD-IC was characterized by UV-vis absorption spectroscopy, fluorescence emission spectroscopy, powder X-ray diffraction, FT-IR, 1H-NMR, TGA and DSC. The phase solubility studies proved that the aqueous solubility of thymol was significantly improved by forming the inclusion complex with HPβCD, and the thermal stability analysis showed that thymol/HPβCD-IC had a better thermal stability than pure thymol. The in vitro antifungal activities of thymol/HPβCD-IC against Botrytis cinerea, Penicillium digitatum and Alternaria alternata were significantly improved compared with pure thymol. Furthermore, the gray mold rot of tomatoes was evidently inhibited by thymol/HPβCD-IC treatment in vivo study. Therefore, the complexation with HPβCD assisted by ultrasound is a promising approach to solubilize and stabilize thymol for application as an antifungal agent in fruit preservation.
- Published
- 2021
25. Encapsulation of quercetin in β-cyclodextrin and (2-hydroxypropyl)-β-cyclodextrin cavity: In-vitro cytotoxic evaluation
- Author
-
Rajaram Rajamohan, Arumugam Praveena, and Samikannu Prabu
- Subjects
chemistry.chemical_classification ,Polymers and Plastics ,Cyclodextrin ,Chemistry ,02 engineering and technology ,General Chemistry ,Powder xrd ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,In vitro ,0104 chemical sciences ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Breast cancer cell line ,Materials Chemistry ,Ceramics and Composites ,Cytotoxic T cell ,0210 nano-technology ,Quercetin ,Nuclear chemistry - Abstract
The formation of host-guest inclusion complex of Quercetin (QRC) with β-Cyclodextrin (β-CD) and (2-Hydroxypropyl)-β-Cyclodextrin (HP-β-CD) is prepared by various methods such as physical method (PM), kneading method (KM) and co-precipitation method (CP). The solid inclusion complex is characterized by UV, Fluorescence, FT-IR, SEM, powder XRD and TG/DTA analysis. The cytotoxic activity of the solid complex is performed against breast cancer cell line and it is noticed that there is better activity than the QRC alone. Hence, the solid complex showed an improvement in the anticancer activity against MDA MB 231 cell line.
- Published
- 2017
26. Preparation of inclusion complex of praziquantel with 2-hydroxypropyl-β-cyclodextrin and pharmacokinetic property improvement
- Author
-
Chamakura V.N.S. Vara Prasad, Pang Yuchang, Yili Ding, Bingyun Wang, and Wutong Cui
- Subjects
Inclusion complex ,Aqueous solution ,Chromatography ,Chemistry ,General Chemical Engineering ,2-Hydroxypropyl-β-cyclodextrin ,Cmax ,Pharmacokinetic property evaluation ,General Chemistry ,Praziquantel ,2 hydroxypropyl β cyclodextrin ,Pharmacokinetics ,In vivo ,Proton NMR ,medicine ,Solubility ,QD1-999 ,medicine.drug - Abstract
An inclusion complex of praziquantel with 2-hydroxypropyl-β-cyclodextrin was designed and prepared by an optimized method and the inclusion complex was characterized by infrared absorption spectral, proton NMR spectral and scanning electron image studies. It was shown that the aqueous solubility of praziquantel has increased (~104 fold) in comparison with praziquantel alone, which is the best result so far for praziquantel solubility study. The in vivo pharmacokinetic properties of praziquantel in dogs such as Cmax, Tmax, AUC0-∞, and t1/2 have been improved significantly after complexation. The increased water solubility of the praziquantel in the complex resulted in the improved values of Cmax (4.82 μg/mL vs 0.51 μg/mL, 9.45 fold higher) and AUC0-∞ (98.06 μg h/mL vs 20.63 μg h/mL, 4.75 fold higher) in dog pharmacokinetic studies, which is useful to find a new praziquantel formulation as an anti-schistosomal agent.
- Published
- 2021
27. 2-Hydroxypropyl-β-cyclodextrin encapsulates dimethyl disulfide producing a controlled release formulation
- Author
-
Ouyang Canbin, Yuan Li, Qiuxia Wang, Dongdong Yan, Xiujun Tang, Lirui Ren, Hongyan Cheng, Bin Huang, Baoqiang Hao, and Aocheng Cao
- Subjects
chemistry.chemical_classification ,Environmental Engineering ,Bromomethane ,010504 meteorology & atmospheric sciences ,Cyclodextrin ,Chemistry ,Environmental pollution ,010501 environmental sciences ,01 natural sciences ,Pollution ,Controlled release ,2-Hydroxypropyl-beta-cyclodextrin ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Odor ,Reduced toxicity ,Delayed-Action Preparations ,Spectroscopy, Fourier Transform Infrared ,Humans ,Environmental Chemistry ,Organic chemistry ,Dimethyl disulfide ,Disulfides ,Waste Management and Disposal ,0105 earth and related environmental sciences - Abstract
Dimethyl disulfide (DMDS), a soil fumigant, is an effective, broad-spectrum compound that often replaces bromomethane (MB) in the prevention and treatment of soil-borne diseases. However, the disadvantages of DMDS include toxicity, volatility, pungent odor, risk of human exposure, and environmental pollution. Cyclodextrin (CD) has been widely used as a carrier of chemicals in many industries due to its functional advantages and safety. In this study, a DMDS-controlled release formulation was developed by encapsulating DMDS in the cavity of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD). This formulation reduced DMDS usage and production costs. Orthogonal experimental design, Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM), Thermal gravity analysis (TGA) characterization, efficacy comparison, safety, and other aspects of the evaluation showed that under the best preparation conditions, the encapsulation rate was 81.49%. The efficacy of DMDS@HP-β-CD was similar to unformulated DMDS. The efficacy duration of the formulation was about two times longer than DMDS, and it was safer to use. This study reveals a cyclodextrin-DMDS formulation with reduced toxicity, longer duration, environmental safety and sustainability.
- Published
- 2021
28. Effect of (2-hydroxypropyl)-β-cyclodextrin on the solubility and in vivo behavior of the myocardial perfusion imaging agent [18F] FRho6G-DEG
- Author
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James A. H. Inkster, Peter Morriss, and Alan B. Packard
- Subjects
Cancer Research ,Myocardial perfusion imaging ,Chromatography ,2 hydroxypropyl β cyclodextrin ,medicine.diagnostic_test ,In vivo ,Chemistry ,medicine ,Molecular Medicine ,Radiology, Nuclear Medicine and imaging ,Solubility - Published
- 2021
29. Silylation of 2-hydroxypropyl-β-cyclodextrin
- Author
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M. K. Grachev, G. I. Kurochkina, I. I. Levina, and A. V. Popkov
- Subjects
Reaction conditions ,2 hydroxypropyl β cyclodextrin ,Silylation ,010405 organic chemistry ,Chemistry ,Organic chemistry ,General Chemistry ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences - Abstract
The effect of the reaction conditions or the nature of the silylating agents on the silylation of hydroxypropyl-β-cyclodextrin with tert-butyldimethylchloro- and diphenyl-methylchlorosilanes is investigated.
- Published
- 2017
30. Inclusion complex of ITH12674 with 2-hydroxypropyl-β-cyclodextrin: Preparation, physical characterization and pharmacological effect
- Author
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Ana Ruiz-Nuño, Sheila Abril, Patrycja Michalska, Izaskun Buendia, Aneta Wojnicz, and Rafael León
- Subjects
Antioxidant ,Polymers and Plastics ,medicine.medical_treatment ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,2 hydroxypropyl β cyclodextrin ,Isothiocyanates ,Phase (matter) ,Spectroscopy, Fourier Transform Infrared ,Materials Chemistry ,medicine ,Organic chemistry ,Melatonin ,Aqueous solution ,Calorimetry, Differential Scanning ,Chemistry ,beta-Cyclodextrins ,Organic Chemistry ,021001 nanoscience & nanotechnology ,Combinatorial chemistry ,2-Hydroxypropyl-beta-cyclodextrin ,0104 chemical sciences ,Solubility ,Mechanism of action ,Active compound ,Proton NMR ,Inclusion (mineral) ,medicine.symptom ,0210 nano-technology - Abstract
ITH12674 is a multitarget drug, designed to exert a dual "drug-prodrug" mechanism of action, able to induce the phase II antioxidant and anti-inflammatory response for the treatment of brain ischemia. However, its physicochemical properties limit its potential preclinical development due to its low water solubility and instability towards heat and pH variations. In order to improve its properties, we prepared the inclusion complex of ITH12674 with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) by the freeze-drying method. The formation of the inclusion complex was confirmed by FT-IR spectroscopy, PXRD, DSC, 1H NMR and SEM techniques. Experimental results showed that the inclusion complex enhanced its water solubility and stability against heat, acidic and basic conditions. Furthermore, the inclusion complex, prepared in water solution, exerted the same potency to induce the phase II antioxidant response as the pure ITH12674. Thus the formation of the inclusion complex with HP-β-CD is a very effective method to stabilize and solubilize the active compound for its future preclinical development.
- Published
- 2017
31. Cavity Closure of 2-Hydroxypropyl-β-cyclodextrin: Replica Exchange Molecular Dynamics Simulations
- Author
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Jintawee Kicuntod, Manaschai Kunaseth, Nawee Kungwan, Khanittha Kerdpol, Seiji Mori, Hisashi Okumura, Thanyada Rungrotmongkol, Chompoonut Rungnim, and Peter Wolschann
- Subjects
Work (thermodynamics) ,Molecular dynamics ,Crystallography ,Conformational change ,2 hydroxypropyl β cyclodextrin ,Chemistry ,Replica ,Radius of gyration ,Solubility ,general_theoretical_chemistry - Abstract
2-Hydroxypropyl-β-cyclodextrin (HPβCD) has unique properties to enhance the stability and the solubility of low water-soluble compounds by inclusion complexation. Understanding of the structural properties of HPβCD and its derivatives based on the number of 2-hydroxypropyl (HP) substituents at the a-D-glucopyranose subunits is rather important. In this work, replica exchange molecular dynamics simulations were performed to investigate the conformational changes of single- and double-sided HP-substitution called as 6-HPβCDs and 2,6-HPβCDs, respectively. The results show that glucose subunits in both 6-HPβCDs and 2,6-HPβCDs have lower chance to flip than in βCD. Also, HP groups are occasionally blocking the hydrophobic cavity of HPβCDs, thus hindering the drug inclusion. We found that HPβCDs with high number of HP-substitutions are more likely to be blocked, while HPβCDs with double-sided HP-substitution are even more probable to be blocked. Overall, 6-HPβCDs with three and four HP-substitutions are highlighted as the most suitable structures for guest encapsulation based on our conformational analyses such as structural distortion, radius of gyration, circularity and cavity self-closure of the HPβCDs.
- Published
- 2019
32. Characterization of in vitro and in vivo bioactivity of a ferulic acid-2-Hydroxypropyl-β-cyclodextrin inclusion complex
- Author
-
Song Cu Yu, Fuu Jen Tsai, Chin Mu Hsu, and Yuhsin Tsai
- Subjects
Coumaric Acids ,Cell Survival ,Biocompatible Materials ,02 engineering and technology ,01 natural sciences ,Ferulic acid ,chemistry.chemical_compound ,Mice ,Colloid and Surface Chemistry ,Differential scanning calorimetry ,2 hydroxypropyl β cyclodextrin ,In vivo ,Cell Line, Tumor ,Malondialdehyde ,0103 physical sciences ,Animals ,Humans ,Physical and Theoretical Chemistry ,Carbon Tetrachloride ,Alternative methods ,Mice, Inbred ICR ,010304 chemical physics ,Superoxide Dismutase ,Water ,Surfaces and Interfaces ,General Medicine ,021001 nanoscience & nanotechnology ,Combinatorial chemistry ,In vitro ,Bioavailability ,2-Hydroxypropyl-beta-cyclodextrin ,Solutions ,Oxidative Stress ,chemistry ,Liver ,Solubility ,Proton NMR ,Hepatocytes ,Chemical and Drug Induced Liver Injury ,0210 nano-technology ,Glycoconjugates ,Hydrophobic and Hydrophilic Interactions ,Biotechnology - Abstract
Ferulic acid (FA) belongs to the family of phenolic acids and exhibits a wide variety of biological activities. However, the bioavailability of FA is not optimal, owing to its limited aqueous solubility. Several methods have been developed to increase FA bioavailability and enhance its cytoprotective effects. Complexing FA with cyclodextrins (CDs) may provide an alternative method to approach these goals. In this study, we prepared an FA-2-hydroxypropyl-β-CD (FA-HP-β-CD) complex, at a 1:1 M ratio of FA to HP-β-CD, which was characterized by 1H NMR, two-dimensional rotating frame spectroscopy and differential scanning calorimetry. Aqueous solubility of FA was improved after complexing with HP-β-CD. Furthermore, in vitro and in vivo experimental results indicated that the FA-HP-β-CD complex had greater bioactivity than FA alone. Therefore, we can conclude that the limitations of FA usage due to low aqueous solubility and bioavailability can be overcome by creating an HP-β-CD inclusion complex with the hydrophobic FA.
- Published
- 2019
33. Cavity Closure of 2-Hydroxypropyl-β-Cyclodextrin: Replica Exchange Molecular Dynamics Simulations
- Author
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Seiji Mori, Hisashi Okumura, Khanittha Kerdpol, Nawee Kungwan, Manaschai Kunaseth, Thanyada Rungrotmongkol, Jintawee Kicuntod, Chompoonut Rungnim, and Peter Wolschann
- Subjects
Conformational change ,Polymers and Plastics ,Chemistry ,Replica ,technology, industry, and agriculture ,General Chemistry ,Article ,carbohydrates (lipids) ,lcsh:QD241-441 ,Crystallography ,Molecular dynamics ,2 hydroxypropyl β cyclodextrin ,2-hydroxypropyl-β-cyclodextrin (HPβCD) ,conformational change ,stomatognathic system ,lcsh:Organic chemistry ,cavity self-closure ,polycyclic compounds ,Radius of gyration ,replica exchange molecular dynamics (REMD) ,Solubility - Abstract
2-Hydroxypropyl-&beta, cyclodextrin (HP&beta, CD) has unique properties to enhance the stability and the solubility of low water-soluble compounds by inclusion complexation. An understanding of the structural properties of HP&beta, CD and its derivatives, based on the number of 2-hydroxypropyl (HP) substituents at the &alpha, d-glucopyranose subunits is rather important. In this work, replica exchange molecular dynamics simulations were performed to investigate the conformational changes of single- and double-sided HP-substitution, called 6-HP&beta, CDs and 2,6-HP&beta, CDs, respectively. The results show that the glucose subunits in both 6-HP&beta, CDs have a lower chance of flipping than in &beta, CD. Also, HP groups occasionally block the hydrophobic cavity of HP&beta, CDs, thus hindering drug inclusion. We found that HP&beta, CDs with a high number of HP-substitutions are more likely to be blocked, while HP&beta, CDs with double-sided HP-substitutions have an even higher probability of being blocked. Overall, 6-HP&beta, CDs with three and four HP-substitutions are highlighted as the most suitable structures for guest encapsulation, based on our conformational analyses, such as structural distortion, the radius of gyration, circularity, and cavity self-closure of the HP&beta, CDs.
- Published
- 2019
34. 2-Hydroxypropyl-β-Cyclodextrin Aggregates: Identification and Development of Analytical Techniques
- Author
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André Rodrigues Sá Couto, Thorsteinn Loftsson, Alexey Ryzhakov, Faculty of Pharmaceutical Sciences (UI), Lyfjafræðideild (HÍ), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Háskóli Íslands (HÍ), and University of Iceland (UI)
- Subjects
Lyfjafræði ,2-hydroxypropyl-β-cyclodextrin ,critical aggregation concentration ,02 engineering and technology ,lcsh:Technology ,030226 pharmacology & pharmacy ,Article ,Surface tension ,03 medical and health sciences ,Aggregation ,Critical aggregation concentration ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Dynamic light scattering ,Osmometer ,General Materials Science ,lcsh:Microscopy ,lcsh:QC120-168.85 ,Chromatography ,lcsh:QH201-278.5 ,lcsh:T ,Chemistry ,aggregation ,Viscometer ,Permeation ,021001 nanoscience & nanotechnology ,lcsh:TA1-2040 ,Efnasambönd ,Nanoparticles ,lcsh:Descriptive and experimental mechanics ,permeation ,nanoparticles ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,lcsh:Engineering (General). Civil engineering (General) ,0210 nano-technology ,lcsh:TK1-9971 - Abstract
It is extremely important for pharmaceutical formulators to have analytical methodology that provides efficient detection and quantification of HP&beta, CD aggregates. Five different methods were then evaluated for their potential to detect these aggregates and to determine critical aggregation concentration (cac): osmometry, viscometry, tensiometry, dynamic light scattering (DLS), and permeability studies. Overall, tensiometry was an inadequate method with which to study HP&beta, CD aggregation, since the addition of HP&beta, CD to water resulted in only minor changes in surface tension. Osmolality and viscosity studies have shown that for HP&beta, CD, solute&ndash, solvent interactions are the main contributors for the observed deviation from ideality. These deviations might be related to the presence of aggregates. The DLS method proved to be an effective method with which to detect HP&beta, CD aggregates and estimate their hydrodynamic diameter, although it presented some limitations concerning their quantification. In terms of the assessed methods, permeation studies were shown to be the best to study HP&beta, CD aggregation phenomena, since they were the only method where the detection of aggregates and the determination of apparent cac values was possible. Also, it was the least invasive for the HP&beta, CD samples and the method that provided more conclusive data. Results suggested that HP&beta, CD, as expected, has less tendency to form aggregates than &beta, CD.
- Published
- 2018
35. Study of the solubility, photostability and structure of inclusion complexes of carvedilol with β-cyclodextrin and (2-hydroxypropyl)-β-cyclodextrin
- Author
-
Ivana Savic-Gajic, Mirjana Popsavin, Agneš Kapor, Ljubiša Nikolić, Vesna Nikolić, and Ivan M. Savic
- Subjects
chemistry.chemical_classification ,Chromatography ,Cyclodextrin ,Chemistry ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,030226 pharmacology & pharmacy ,03 medical and health sciences ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Stability constants of complexes ,Molar ratio ,Phase (matter) ,medicine ,Solubility ,0210 nano-technology ,Carvedilol ,Food Science ,Nuclear chemistry ,medicine.drug - Abstract
Carvedilol is one of the most effective antihypertensive drugs used in the treatment of congestive heart failure. A major disadvantage of this pharmaceutical active substance is its limited solubility in water, gastric and intestinal fluids. One way to overcome this problem is the preparation of inclusion complexes. The aim of this study was to prepare the inclusion complexes of carvedilol with β-cyclodextrin (β-CD) and (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) and to investigate their physical properties. The formation of inclusion complexes with β-CD and HP-β-CD was confirmed using FTIR, 1H-NMR and XRD methods. Phase solubility studies indicate the formation of inclusion complexes in 1:2 molar ratio and the increase of carvedilol solubility. The stability constant (β 2) was found to be 3.4 × 104 and 5.1 × 104 M−2 for inclusion complexes of carvedilol:β-CD and carvedilol:HP-β-CD, respectively. Photostability of carvedilol was increased after preparation of inclusion complexes with β-CD and HP-β-CD. Based on the results of this study, it can be concluded that the prepared complexes of carvedilol improve the solubility and stability of carvedilol and give it an advantage to be applied for the design of new pharmaceutical formulations.
- Published
- 2016
36. Effect of 2-Hydroxypropyl-β-cyclodextrin on the diffusion behaviour of L-dopa in aqueous solutions
- Author
-
Miguel A. Esteso, M. Luísa Ramos, Derek G. Leaist, Marisa C.F. Barros, Patricia A.M. Prazeres, Ana C.F. Ribeiro, and Hugh D. Burrows
- Subjects
1h nmr spectroscopy ,Aqueous solution ,Chemistry ,Diffusion ,Taylor dispersion ,Analytical chemistry ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,3. Good health ,0104 chemical sciences ,2 hydroxypropyl β cyclodextrin ,020401 chemical engineering ,General Materials Science ,0204 chemical engineering ,Physical and Theoretical Chemistry ,Concentration gradient ,Ternary operation - Abstract
Ternary mutual diffusion coefficients (D11, D22, D12 and D21) measured by the Taylor dispersion method are reported for aqueous solutions of levodopa (L-dopa) (1) in the presence of 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) (2) at 25 °C and 37 °C and concentrations up to 0.005 mol · dm−3. The effects of HP-β-CD (HP-β-CD) on the transport of L-dopa are assessed by comparing the binary diffusion coefficient of aqueous L-dopa solutions with the main diffusion coefficient (D11) measured for ternary {L-dopa (1) + HP-β-CD (2)} solutions. 1H NMR spectroscopy suggests no significant complexation between HP-β-CD and L-dopa. However, coupled diffusion of L-dopa and HP-β-CD occurs, as indicated by non-zero values of the cross-diffusion coefficients, D12 and D21. HP-β-CD concentration gradients produce significant co-current coupled flows of L-dopa.
- Published
- 2016
37. Metal-free thermally-responsive pseudohybrid nanoparticles based on 2-hydroxypropyl-β-cyclodextrin
- Author
-
A. N. Zakharov, V. V. Spiridonov, Irina N. Topchieva, and I. G. Panova
- Subjects
Aqueous solution ,Chemistry ,General Chemical Engineering ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Phenolphthalein ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Metal free ,Chemical engineering ,Local Hyperthermia ,Yield (chemistry) ,Drug delivery ,0210 nano-technology - Abstract
Pseudohybrid nanoparticles of 800 nm in diameter based on self-assembled high-substituted 2-hydroxypropyl-β-cyclodextrin fabricated in the presence of iron(II) salts were found to be thermally responsive within narrow range of temperature. As-prepared metal-free nanoparticles are stable in aqueous solution at room temperature. However, the increase in temperature results in break-like collapse of nanoparticles to yield species of 400 nm in diameter. On the example of phenolphthalein, it was shown that nanoparticles loaded with model drug decompose to release superficial guest-host inclusion complexes. Local hyperthermia provokes nanoparticles decomposing and drug releasing that allows recognizing focuses of pathology in human body. It might be used for diagnostic medicinal aims and be also considered as basis for the construction of intravascular drug delivery/release systems.
- Published
- 2016
38. Effects of sulfamethoxazole-trimethoprim associated to resveratrol on its free form and complexed with 2-hydroxypropyl-β-cyclodextrin on cytokines levels of mice infected by Toxoplasma gondii
- Author
-
Maria Rosa Chitolina Schetinger, Virginia Cielo Rech, Nathieli B. Bottari, Matheus D. Baldissera, Marta M.M.F. Duarte, Aleksandro Schafer da Silva, Camila Tochetto, Alexandre A. Tonin, Giovana Camillo, Vivian S.K. Nishihira, Gustavo R. Thomé, Rafael A. Fighera, Vera Maria Morsch, and Fernanda Silveira Flores Vogel
- Subjects
Serum ,Drug ,media_common.quotation_subject ,Antiprotozoal Agents ,Biology ,Pharmacology ,Resveratrol ,Microbiology ,Group B ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Stilbenes ,Trimethoprim, Sulfamethoxazole Drug Combination ,Pi ,Animals ,Immunologic Factors ,Sulfamethoxazole/Trimethoprim ,media_common ,Mice, Inbred BALB C ,beta-Cyclodextrins ,Brain ,Toxoplasma gondii ,biology.organism_classification ,2-Hydroxypropyl-beta-cyclodextrin ,Toxoplasmosis, Animal ,Treatment Outcome ,Infectious Diseases ,chemistry ,Immunology ,Cytokines ,Free form ,Toxoplasma - Abstract
The aim of this study was to investigate the effects of resveratrol on its free form and complexed with 2-hydroxypropyl-β-cyclodextrin (HPβCD) when associated with sulfamethoxazole-trimethoprim (ST) on cytokines levels of mice (n = 60) experimentally infected by Toxoplasma gondii. Groups A and E were used as controls (untreated): negative and positive, respectively. The onset of treatment started 20 days post-infection (PI), and it lasted for 10 consecutive days. ST was administered orally in doses of 0.5 mg kg(-1) for groups B and F, while 100 mg kg(-1) was the dose for resveratrol in its free form (groups C - G), inclusion complex (groups D and H), and on free and inclusion complex together (groups I - J). On day 31 PI, blood samples were collected in order to evaluate the cytokine profile. The mice that received drug combination (I and J) showed a significant (P < 0.05) reduction in the number of cysts in the brain compared to other infected groups (E - H). The results showed that mice from the Group E had increased (P < 0.001) levels of pro-inflammatory cytokines, while IL-10 levels were reduced when compared to the Group A. Additionally, there were increased levels of IL-4 and IFN-γ in animals of groups C and D, respectively (P < 0.05). Animals of the Group B showed reduced levels of IL-1, IL-4, IL-6, TNF-α, and IFN-γ (P < 0.05). Mice infected and treated (groups F - J) showed increased levels of pro-inflammatory cytokines along with a reduction of IL-10. Treatment with the combination of drugs (the Group J) led to a protective effect, i.e. reduction in pro-inflammatory cytokines. Therefore, resveratrol associated with ST was able to modulate seric cytokine profile and moderate the tissue inflammatory process caused by T. gondii infection, as well as to reduce parasite multiplication.
- Published
- 2015
39. An improved method for the preparation of β-lapachone:2-hydroxypropyl-β-cyclodextrin inclusion complexes
- Author
-
Fernando de C. da Silva, Marcella de Sá Haddad Queiroz, Carolina G. S. Lima, Caroline D. Nicoletti, Débora Omena Futuro, and Vitor F. Ferreira
- Subjects
β lapachone ,Chemistry ,Pharmaceutical Science ,Liquid phase ,Improved method ,02 engineering and technology ,021001 nanoscience & nanotechnology ,030226 pharmacology & pharmacy ,Combinatorial chemistry ,Bioavailability ,03 medical and health sciences ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Thermal instability ,Solubility ,0210 nano-technology ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Ortho-naphthoquinone β-lapachone, a compound originally isolated from the Tabebuia sp tree, has showed pronounced activity against different parasites and cancer cells. However, its use presents limitations mostly related to its low bioavailability, solubility, thermal instability and photodecomposition. In order to overcome such disadvantages, this work aimed to evaluate different methodologies to promote the formation of inclusion complexes between β-lapachone and 2-hydroxypropyl-β-cyclodextrin. For this purpose, manual and automated mechanochemical processes as well as liquid phase methods were employed. The complexes were evaluated for entrapment efficiency (EE) and stability, that is, the maintenance of the entrapment over time. The samples which showed the best results were submitted to a thorough physicochemical and spectroscopic characterization, including 2D NMR techniques, and such analyses revealed that the mechanochemical process furnished the most stable inclusion complex.
- Published
- 2020
40. Betulin/2-hydroxypropyl-β-cyclodextrin inclusion complex: Physicochemical characterization and hepatoprotective activity
- Author
-
Wieslawa Misiuk, Artur Stepniak, Bayarmaa Erdenebayar, T. V. Ilyich, Elena Belonovskaya, Oxana J. Lukivskaya, Sluken Rakhmadieva, Irina Kuzmitskaya, Bartłomiej Pałecz, Siarhei Kirko, Elena Naruta, Ilya Zavodnik, Alexej Shlyahtun, and Vyacheslav Buko
- Subjects
chemistry.chemical_classification ,Betulin ,Enthalpy ,Infrared spectroscopy ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Triterpene ,chemistry ,Materials Chemistry ,Titration ,Physical and Theoretical Chemistry ,Inclusion (mineral) ,0210 nano-technology ,Spectroscopy ,Stoichiometry ,Nuclear chemistry - Abstract
The thermodynamic and spectroscopic parameters of the betulin/2-hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complex and its hepatoprotective activity were investigated in rats with nonalcoholic steatohepatitis (NASH). The pentacyclic triterpene betulin was isolated from birch bark and complexed with HPβCD. The parameters of inclusion complex formation were evaluated by infrared spectroscopy and differential scanning and isothermal titration calorimetries. The aqueous solubility and stability of betulin were significantly increased due to the formation of a stable inclusion complex with a stoichiometry of 1:1. The determined changes in molar enthalpy (ΔH 0) and free energy (ΔG
- Published
- 2020
41. Linking the concentrations of itraconazole and 2-hydroxypropyl-β-cyclodextrin in human intestinal fluids after oral intake of Sporanox®
- Author
-
Patrick Augustijns, Govert W. Somsen, Elena Domínguez-Vega, Joachim Brouwers, Jef Stappaerts, Philippe Berben, Matthias J.A. Vink, BioAnalytical Chemistry, and AIMMS
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Adult ,Male ,Antifungal Agents ,Itraconazole ,Duodenum ,Drinking ,Pharmaceutical Science ,Administration, Oral ,02 engineering and technology ,030226 pharmacology & pharmacy ,Excipients ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,2 hydroxypropyl β cyclodextrin ,Tandem Mass Spectrometry ,medicine ,Humans ,Tissue Distribution ,Water intake ,Solubility ,LC-MS/MS ,chemistry.chemical_classification ,Chromatography ,Cross-Over Studies ,Cyclodextrin ,Intestinal Secretions ,Chemistry ,Human intestinal fluids (HIF) ,Hydroxypropyl-β-cyclodextrin (HP-β-CD) ,General Medicine ,SDG 10 - Reduced Inequalities ,Permeation ,021001 nanoscience & nanotechnology ,2-Hydroxypropyl-beta-cyclodextrin ,Clinical trial ,medicine.anatomical_structure ,Free fraction ,Female ,Caco-2 Cells ,0210 nano-technology ,Biotechnology ,medicine.drug ,Chromatography, Liquid - Abstract
In a previously performed small-scale clinical study, healthy volunteers were asked to ingest an oral solution of itraconazole (Sporanox®) containing 40% 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) (i) with or (ii) without a standardized volume of water (240 mL) after which gastrointestinal and blood samples were collected. Although omitting water during the administration of Sporanox® resulted in noticeably higher duodenal concentrations of itraconazole, systemic exposure was almost unaffected. It is assumed that this discrepancy can be explained by differences in the extent of entrapment of itraconazole in the duodenum caused by differential complexation depending on the concentration of cyclodextrins. To further substantiate this hypothesis, the quantification of HP-β-CD concentrations in the aspirated intestinal fluids was performed by LC-MS/MS. When comparing the intestinal concentrations of itraconazole and HP-β-CD for one single healthy volunteer (HV02) in both test conditions, an excellent correlation was observed (Spearman's rank coefficient of 0.96). Moreover, the data suggest that, similar to aqueous buffer media, also in human intestinal fluids a non-linear relationship exists between itraconazole solubility and HP-β-CD concentration (Ap-type profile; Spearman's rank coefficient of 0.78), indicating that higher order complexes are formed at higher concentrations of HP-β-CD. This difference in extent of entrapment in the inclusion complexes helps to understand the observed impact of water intake on precipitation and permeation behavior of itraconazole in man. Without water intake, higher HP-β-CD concentrations resulted in less precipitation and increased duodenal concentrations of itraconazole. On the other hand, the stronger interaction at higher HP-β-CD concentrations reduced the free fraction of the drug explaining that increased intraluminal concentrations of itraconazole were not translated into an enhanced uptake. In conclusion, quantifying the concentrations of the solubilizing agent HP-β-CD in human intestinal fluids appeared to be of crucial importance to interpret the intraluminal behavior of an orally administered cyclodextrin-based solution. ispartof: EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS vol:13 pages:231-236 ispartof: location:Netherlands status: published
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- 2018
42. Hydroxyapatite Functionalized with 1,2-Hydroxypropyl-β-Cyclodextrin as Scaffolds for Antibiotic Release in Bone Tissue
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Francisco J. Otero-Espinar, Asteria Luzardo-Álvarez, José Blanco-Méndez, Aloia Gómez-Gratacòs, Beatriz Rey-Aira, and Andrea Freire-Dapena
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Antibiotic release ,chemistry.chemical_classification ,Cyclodextrin ,Chemistry ,Regeneration (biology) ,technology, industry, and agriculture ,Bone healing ,Bone tissue ,medicine.anatomical_structure ,2 hydroxypropyl β cyclodextrin ,medicine ,Solubility ,Bone regeneration ,Nuclear chemistry - Abstract
Background: The difficulty of eliminating bacteria infecting bone tissue during bone healing treatments or surgery makes it desirably to develop solid scaffolds capable of sustained release of antibiotics to achieve the regeneration of the damaged tissue. Methods: With this function in view, in this work we prepared HA scaffolds functionalized with1,2- Hydroxypropyl-β-Cyclodextrin to increase the loading and sustained release for ciprofloxacin. Results: The lowest amount of CD was sufficient to fabricate HA functionalized microparticles and allowing for the CPX loading to be increased more than 40 times with respect to the non-functionalized HA particles. Spray-drying process rendered spherical particles with irregular surface that improved the release and the solubility of low soluble CPX in pH 7.4 medium in form of free drug and cyclodextrin complexes although free cyclodextrins were also released to the medium. Conclusion: It is concluded that the synthesized microparticles based on HA functionalized with Hydroxypropyl -β-Cyclodextrin merit further investigation to be used as platform for delivering antibiotics in bone regeneration.
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- 2018
43. The Development of a New Complexation Technique of Hydrocortisone Acetate with 2-Hydroxypropyl-β-Cyclodextrin: Preparation and Characterization
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Márcio Robert Mattos da Silva, Magaly Girão Albuquerque, Sheila Garcia, Márcia Soares Sader, Elisabete Pereira dos Santos, Rita de Cássia da Silva Assunção Barros, and Jackeline da Silva Coelho Oliveira
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2 hydroxypropyl β cyclodextrin ,Pharmaceutical technology ,Chemistry ,Posttranslational modification ,Pharmaceutics ,Nuclear chemistry ,Hydrocortisone acetate - Published
- 2018
44. Molecular encapsulation of amodiaquine in 2-hydroxypropyl β-cyclodextrin cavity. Characterization and its in vitro cytotoxicity
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Arumugam Anitha, Moorthiraman Murugan, and Rajaram Rajamohan
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Drug ,medicine.drug_class ,media_common.quotation_subject ,In vitro cytotoxicity ,02 engineering and technology ,Amodiaquine ,Mannich base ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Chloroquine ,medicine ,polycyclic compounds ,Spectroscopy ,media_common ,technology, industry, and agriculture ,Molecular encapsulation ,021001 nanoscience & nanotechnology ,Quinolone ,Combinatorial chemistry ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,carbohydrates (lipids) ,chemistry ,0210 nano-technology ,medicine.drug - Abstract
Amodiaquine has been used widely as an antimalarial drug. Amodiaquine is a mannich base 4-amino quinolone with a mode of action similar to that of chloroquine. The inclusion complex of amodiaquine with 2-(hydroxypropyl)-beta-cyclodextrin in solution phase is studied from the ground and excited state with absorption and fluorescence spectroscopic techniques, respectively. A binding constant and stoichiometric ratio between amodiaquine and 2-(hydroxypropyl)-beta-cyclodextrin are calculated by the use of Benesi–Hildebrand equation. The solid complexes are prepared by physical, kneading and coprecipitation methods. The solid complexes are characterized by Fourier-transform infrared spectral analysis, Differential scanning calorimetric curves and powder X-ray diffraction patterns. The anticancer activity was tested for pure amodiaquine and their complex with 2-(hydroxypropyl)-beta-cyclodextrin against MDA MB 231 cell line. It clearly showed that a significant improvement of anticancer activity of amodiaquine when forming a complex with 2-(hydroxypropyl)-beta-cyclodextrin.
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- 2018
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45. Gd3+-1,4,7,10-Tetraazacyclododecane-1,4,7-triacetic-2-hydroxypropyl-β-cyclodextrin/Pluronic Polyrotaxane as a Long Circulating High Relaxivity MRI Contrast Agent
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David H. Thompson, Yawo A. Mondjinou, Zhuxian Zhou, Seok-Hee Hyun, Aditya Kulkarni, and Zheng-Rong Lu
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Materials science ,Rotaxanes ,Stereochemistry ,MRI contrast agent ,Gadolinium ,Contrast Media ,chemistry.chemical_element ,Beta-Cyclodextrins ,Poloxamer ,Article ,Long circulating ,Heterocyclic Compounds, 1-Ring ,Mice ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Coordination Complexes ,Animals ,Tissue Distribution ,General Materials Science ,Cyclodextrins ,Mice, Inbred BALB C ,beta-Cyclodextrins ,Polyrotaxane ,Magnetic Resonance Imaging ,2-Hydroxypropyl-beta-cyclodextrin ,Monomer ,chemistry ,Nuclear chemistry - Abstract
A multivalent magnetic resonance imaging agent based on a 2-hydroxypropyl-β-cyclodextrin (HPCD):Pluronic F127 polyrotaxane carrier has been synthesized, and its blood pool contrast properties have been characterized. This Gd3+-DO3A-HPCD/Pluronic polyrotaxane construct is shown to circulate for more than 30 min and provide100-fold vascular enhancement relative to the monomeric Gd3+-DO3A-HPCD control that is rapidly cleared via the kidney. The high r1 relaxivity at 37 °C (23.83 mM(-1) s(-1) at 1.5 T; 34.08 mM(-1) s(-1) at 0.5 T), extended blood circulation, well-known pharmacology of the polyrotaxane precursors, and absence of acute toxicity make it a highly attractive blood pool contrast agent candidate.
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- 2015
46. Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes
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Linjia Liu, Vivek D. Badwaik, Yawo A. Mondjinou, Aditya Kulkarni, David H. Thompson, and Asher Demoret
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Polymers and Plastics ,Chemistry ,Cationic polymerization ,Bioengineering ,Nanotechnology ,02 engineering and technology ,Transfection ,Gene delivery ,Poloxamer ,Polyrotaxane ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Biomaterials ,Colloid ,2 hydroxypropyl β cyclodextrin ,Materials Chemistry ,Biophysics ,0210 nano-technology ,Intracellular ,Biotechnology - Abstract
A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-β-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density and threading efficiency of the PR(+) agent significantly influenced the colloidal stability and physical properties of the complexes, which impacted their intracellular transfection efficiencies. Taken together, our results suggest that HPCD: Pluronic PR(+) can be used as potent vectors for pDNA-based therapeutics.
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- 2015
47. Intermolecular interactions in rifabutin—2-hydroxypropyl-β-cyclodextrin—water solutions
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Yu. V. Yermolenko, V. Yu. Konyukhov, S. E. Gelperina, V. I. Polshakov, A. V. Anshakova, and Olga Maksimenko
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Rifabutin ,Molecular model ,Chemistry ,Stereochemistry ,Intermolecular force ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Computational chemistry ,Stability constants of complexes ,medicine ,Molecule ,Piperidine ,Physical and Theoretical Chemistry ,medicine.drug - Abstract
The possibility of a intermolecular complex rifabutin (RB)-2-hydroxypropyl-β-cyclodextrin (HP-β-CD) formed as a result of the interaction of the piperidine fragment of the RB molecule and the hydrophobic cavity of the HP-β-CD molecule was found. The stability constant of the intermolecular complex was determined.
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- 2015
48. A novel electrocatalyst with high sensitivity in detecting glutathione reduced by 2-hydroxypropyl-β-cyclodextrin enveloped 10-methylphenothiazine
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Xueni Li, Yimin Wang, Yan Lou, Jiani Liu, Ting Xiao, Longzhen Zheng, and Nan Zhang
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Detection limit ,Chemistry ,10-methylphenothiazine ,General Chemical Engineering ,Analytical chemistry ,General Chemistry ,Glutathione ,Electrochemical detection ,Electrocatalyst ,Experimental research ,Catalysis ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Nuclear chemistry - Abstract
This paper reports on an experimental research of a 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) enveloped 10-methylphenothiazine (MPT) catalyst (MPT-HP-β-CD) with high sensitivity and wonderful electrical conductivity for the electrochemical detection of reduced glutathione (GSH) in a neutral environment. We obtained a linear response range from 10−6 mol L−1 to 5.8 × 10−4 mol L−1 for GSH with a low detection limit of 2.87 × 10−7 mol L−1, providing a potentially usefulness in the development of sensors for non-enzyme detection of GSH.
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- 2015
49. Inhibitory effect of Angelica sinensis extract in the presence of 2-hydroxypropyl-β-cyclodextrin
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Fuu Jen Tsai, Chin Mu Hsu, and Yuhsin Tsai
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Carcinoma, Hepatocellular ,Angelica sinensis ,Polymers and Plastics ,Ferulic acid ,chemistry.chemical_compound ,2 hydroxypropyl β cyclodextrin ,Cell Line, Tumor ,Materials Chemistry ,Humans ,Cytotoxicity ,Inhibitory effect ,Angelica sinensis extract ,Active ingredient ,Chromatography ,Aqueous solution ,Dose-Response Relationship, Drug ,biology ,Chemistry ,Liver Neoplasms ,beta-Cyclodextrins ,Organic Chemistry ,Drug Synergism ,biology.organism_classification ,2-Hydroxypropyl-beta-cyclodextrin ,Drug Combinations ,Drugs, Chinese Herbal - Abstract
Angelica sinensis (AS) is a traditional Chinese medicinal herb. Its ethanolic extract contains active ingredients, such as ferulic acid, ligustilide, and butylidenephthalide, which are hydrophobic and have inhibitory effects on hepatoma cells. To increase the aqueous solubility/dispersibility of AS extract and study the consequent inhibitory effect on hepatoma cells, the ethanolic extract of AS was complexed with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), a cyclic oligosaccharide that has a hydrophilic outer surface and a hydrophobic central cavity. The AS-HP-β-CD complex (weight ratio of AS extract: HP-β-CD=1:5) was prepared and characterized. The effect of complexing the AS extract with HP-β-CD on Hep3B cell growth was investigated by analyzing cytotoxicity. Our results showed that cytotoxicity inhibition of AS-HP-β-CD complex was up to 94% and higher than that of AS extract (about 68%). These observations suggested that the use of HP-β-CD to stabilize AS extract in aqueous solution was possible for herbal medicine application.
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- 2014
50. Photochromism of the natural dye 7,4′-dihydroxy-5-methoxyflavylium (dracoflavylium) in the presence of (2-hydroxypropyl)-β-cyclodextrin
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Nuno Basílio, Fernando Pina, José M. Pedrosa, and Tânia Lopes-Costa
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chemistry.chemical_classification ,Base (chemistry) ,Chemistry ,Kinetics ,Photochemistry ,Chemical reaction ,Pigment ,Photochromism ,2 hydroxypropyl β cyclodextrin ,visual_art ,visual_art.visual_art_medium ,Physical and Theoretical Chemistry ,Natural dye ,Stoichiometry - Abstract
A photochromic system based on dracoflavylium, a natural pigment extracted from Dragon's blood, a resin appearing in the injury parts of the tree Dracaena draco, is studied in water. The photochromism arises from the irradiation of the trans-chalcone, giving a mixture of flavylium cation/quinoidal base as a photoproduct via cis-chalcone and hemiketal. The performance of the photochromic system can be improved in the presence of (2-hydroxypropyl)-β-cyclodextrin. A mathematical model to account for the details of the kinetics and thermodynamics of the system was deduced. The model is general for all the host-guest systems involving the flavylium network of chemical reactions with 1 : 1 stoichiometric association.
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- 2014
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