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In order to study the water trophication of Dongping Lake, six water environment and water ecology survey experiments had been carried out since July 2019. The phytoplankton species, density and physicochemical indicators had been obtained. The trophic status of Dongping Lake was analyzed by using the phytoplankton indicator method, phytoplankton abundance evaluation method and comprehensive trophic state index method. The results show that cyanophyta and chlorophyta are the dominant species during the study period. Different dominant species appear at different monoitoring sites in different periods, but cyanophyta species are the dominant species and all show eutrophication. In general, the water body of Dongping Lake presents light-level of eutrophication. The water body has a higher degree of eutrophication, especially in summer, reaching to the moderate-level. [ABSTRACT FROM AUTHOR]

Phytoremediation is a typical environmentally friendly and economical method to in situ natural remediation of soil pollution. Therefore, it can widely be expected to serve as a very feasible way to remediate the contaminated rice paddy fields with high cadmium accumulation, particularly without changing the property and structure of farmland. This study aims to explore the remediation potential of high Cadmium (Cd) accumulated rice varieties (Yangdao 6 and Yuzhenxiang) in the contaminated farmland soil. A field plot experiment was conducted to investigate the accumulation regularity in rice, removal time and removal height of rice varieties with high cadmium accumulation. The content of Cd was also determined in 5 parts (root, stem 0-10 cm, stem 10-20 cm, stem above 20 cm and grain) of rice straw at 6 different growth stages (seedling, tillering, booting, full heading, ripening and full ripening). The results showed that the content of Cd in different parts of rice (Yangdao 6 and Yuzhenxiang) increased with the prolongation of the growth stage ranging from the booting to the mature stage, where the maximum was observed. Besides, the Cd distribution in different parts of rice decreased with the plant height in the same period. At the mature stage, the Cd content in roots, 0-10 cm stem, 10-20 cm stem, above 20 cm stem, and grain of rice varieties Yangdao 6 and Yuzhenxiang were 19.3, 11.8, 9.4, 8.1, and 3.9 mg/kg, and 19.5, 16.3, 14.3, 9.7 and 3.7 mg/kg, respectively. The Cd enrichment coefficients were also all greater than 1, indicating high Cd accumulation. The removal content of cumulative cadmium was ranked in order of the whole plant > the whole ground part > 10 cm above the ground > 20 cm above the ground, harvested rice straw of Yangdao 6 and Yuzhenxiang during the whole growth period. Specifically, the removal contents of cumulative cadmium in the rice straw of Yangdao 6 and Yuzhenxiang were up to 1 299.90 and 1 278.33 μg/plant, respectively, when the whole shoot was removed at the full maturity stage. The removal contents of cumulative cadmium in the rice straw of Yangdao 6 and Yuzhenxiang were up to 1652.11 and 1 547.70 μg/plant, respectively, when the whole plant was removed at the full maturity stage. The removal efficiency of soil Cd was 9.1% and 8.5%, respectively, when the rice varieties Yangdao 6 and Yuzhenxiang were planted once a year and the whole plant was removed. By contrast, the removal efficiency of soil Cd was 7.2% and 7.1%, respectively, when the whole shoot was removed. It infers that the Cd polluted rice roots were difficult to be removed. Therefore, it was strongly recommended to remove the rice straw by harvesting the whole ground part after the rice was fully ripe, particularly considering the remediation and operability. As such, the remediation of Cd contaminated rice paddy fields can remain the soil structure and local planting pattern in a long term, compared with dryland phytoremediation of Cd polluted farmland. The finding can provide a new insightful idea for the phytoremediation in cadmium-contaminated paddy fields. [ABSTRACT FROM AUTHOR]

Two n-butanol zirconium crosslinkers were prepared using n-butanol zirconium as the central ionic reagent and 3-amino-1, 2-propanediol (APG) and N-methyldiethanolamine (MDEA) as the organic ligands, respectively. A series of water-based fracturing fluids were prepared with konjac glucomannan (KGM) solution and n-butanol zirconium crosslinker The morphology, structure and properties of fracturing fluid are systematically characterized by Fourier transform infrared spectrometry (FT-IR), nuclear magnetic resonance spectroscopy (NMR), high resolution mass spectrometer (HRMS), rheometer, scanning electron microscopy (SEM), and differential scanning calorimeter (DSC) . The reaction pathway of zirconate and KGM crosslinking is discussed. The sol-gel transition point is accurately determined by Winter-Chambon formula. The effects of reactant concentration and ligand species on storage modulus (G'), loss modulus (G″), and solgel transition time (tcr) are studied. It is found that compared with APG ligand, the organozirconium/KGM gel system with MDEA as ligand have better delayed cross-linking performance, better temperature and shear resistance. Its tcr shows a trend of first decreasing and then increasing with the increase of KGM concentration, and a decreasing trend with the increase of ligand concentration and crosslinking agent concentration. When the concentration of KGM solution is controlled at 4. 0 g/L, the delay time of gel system can be adjusted within 7~45 min at room temperature, the sol-gel transition temperature (Tsol-gel) is 71. 4 ℃, and its temperature resistance can reach 120 ℃. The viscosity of 171 mPa·s is maintained at a shear rate of 170 s-1 . The organic zirconium crosslinked KGM gel system provides a useful reference for the research and application in the field of petroleum exploitation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Asparaginase can inhibit angiogenesis and affect blood supply, and make damage to the vascular endothelial cells, thereby affecting the local blood supply of femoral head. OBJECTIVE: To investigate the effect of asparaginase on the avascular necrosis of femoral head induced by dexamethasone. METHODS: Balb/c male mice were provided by Laboratory Animal Center of Dalian Medical University, and the study was approved by the Experimental Ethics Committee of Affiliated Zhongshan Hospital of Dalian University. Sixty male Balb/c mice were randomly divided into four groups: asparaginase + dexamethasone group (n=15, 2 mg/L dexamethasone in the water, and intraperitoneal injection of 1 200 U/kg asparaginase), asparaginase group (n=15, intraperitoneal injection of 1 200 U/kg asparaginase), dexamethasone group (n=15, 2 mg/L dexamethasone in the water), and control group (n=15, no intervention). All mice were killed after 8 weeks. The body mass, coagulation factors, hematoxylin-eosin staining and immunohistochemical staining of the femoral head were observed. RESULTS AND CONCLUSION: (1) The body mass gain in the asparaginase + dexamethasone group was significantly lower than that in the other three groups. (2) The levels of coagulation factor III and V in the asparaginase + dexamethasone group were significantly higher than those in the other three groups (P < 0.05 or P < 0.01). (3) Compared with the other three groups, the empty bone lacunae rate was increased (P < 0.05), and trabecular fracture rate was decreased (P < 0.05), and the degree of osteonecrosis was more obvious in the asparaginase + dexamethasone group. (4) The absorbance value of osteoprotegerin in the asparaginase + dexamethasone group was significantly lower than that in the other three groups (P < 0.05 or P < 0.01). (5) These results indicate that asparaginase can promote avascular necrosis of femoral head in mice. [ABSTRACT FROM AUTHOR]

Background The obsessive-compulsive disorder （OCD） features complexity in etiological factors and high heterogeneity in clinical manifestations. OCD patients with different ages of onset vary in clinical symptoms and etiology. However, current studies on inpatients with early- and late-onset OCD are limited. Objective To explore the differences in clinical characteristics between early- and late-onset OCD inpatients as well as the factors affecting the onset age of OCD, so as to provide references for early screening and treatment of OCD patients. Methods This study was based on collected medical records of 540 patients with OCD who received inpatient treatments at the Affiliated Brain Hospital of Nanjing Medical University between March 2012 and March 2023. Patients with onset age above 18 were placed into early-onset group(n=310) and the others into late-onset group (n=230). Then differences in demographic data and clinical symptoms between two groups of patients were compared. Binary logistic regression was used to analyze the factors that affect the onset age of OCD. Results Observing the demographic data, there were significant differences between the two groups in the results in gender, marital status, family history of mental illness, ratio of comorbidities with other mental illnesses, occupational composition, education level and types of obsessive-compulsive symptoms(χ² =22. 302、170. 556, 9. 224, 13. 624, 242. 277, 59. 791, 7. 231, P<0. 05 or 0. 01). Also, the results in ages of onset and hospitalization between two groups were significantly different(Z=-19. 915, 16. 831, P<0. 01). In terms of clinical symptoms, the early onset group had a higher proportion of symptoms including obsessive thinking (χ² =11. 998, P<0. 05), ordering(χ² =7. 731, P<0. 05)and rituals(χ² =7. 714, P<0. 05), while the proportion of obsessive checking(χ² =8. 204, P<0. 05) and washing (χ² =7. 506, P<0. 05) symptoms were relatively low. In terms of risk factors, there were several independent risk factors that influence the onset age of OCD inpatients, including comorbid neurodevelopmental disorder, comorbid affective disorder, family history of schizophrenia and family history of affective disorder (OR=19. 587, 1. 830, 3. 065, 4. 431, P<0. 05). Among them, comorbid neurodevelopmental disorder was the core influencing factor, and female gender was a protective factor for early-onset patients(OR=0. 417, P<0. 01). Conclusion There are differences in demographic data and clinical symptom characteristics between early- and late-onset OCD inpatients, and comorbid neurodevelopmental disorder plays as a core risk factor affecting the onset age of OCD inpatients. [Funded by Jiangsu Province Key Research and Development Plan for Social Development Special Project(number, BE2021616)； Jiangsu Province Social Development General Project (number, BE2022678); Key Project of Nanjing Medical Science and Technology Development Fund(number, ZKX20029)]. [ABSTRACT FROM AUTHOR]

Reservoir impoundment has changed the temporal and spatial distribution of water temperature and caused ecological problems, such as ecosystem disorder and low- temperature underwater discharge in reservoir areas. This resulted in adverse effects on the growth and reproduction of indigenous fish. The 3-D water temperature model was established using the Delft3D model. The dynamic change process of water temperature in the transverse, longitudinal, and vertical dimensions of the Nuozhadu Reservoir under typical meteorological and hydrological conditions was simulated. Considering this context, and combined with the ecological water temperature demand of downstream fish, a layered water intake scheme of stacked beam doors with ecological water temperature as the goal was formulated. The most suitable location and time for releasing indigenous fish ( Tor sinensis) in Nuozhadu Reservoir were determined by combining the requirements of the species involving breeding and release. It was shown in the results that there are differences in the layered water intake scheme of stop logs in different years and the suitable location and time for proliferation and release. The universal layered water intake rules for stop logs were formulated based on the water temperature demand in typical years and the engineering conditions of stop logs. The most suitable location for placing Chinese snakehead fry was the Xiaoheijiang tributary, preferably from August to September. A scientific basis for developing a reservoir ecological dispatching strategy to alleviate the water temperature imbalance may be provided by the results of this study. [ABSTRACT FROM AUTHOR]

Traditional Chinese medicine(TCM) is based on the belief that the transformation of qi and blood strength and weakness is the process of human growth and aging, while the disorder of qi and blood is the root of the development of disease. " Women take blood as the basis and qi for use. " Menstruation, pregnancy, birth, and breast milk are based on qi and blood. Postpartum women are often in the states of qi and blood deficiency and blood stasis due to qi deficiency. In older women, the liver blood and kidney essence gradually decline, and qi and blood are also insufficient. According to the characteristics of the population and symptoms of female stress incontinence, it is believed that disorder of qi and blood is the basic pathogenesis of the disease, and the diagnosis should be based on the characterization of qi and blood. It is believed that the disorder of qi and blood in the five organs is the basis of the pathology, while stasis and dampness are important pathological products of the development of female stress incontinence. Therefore, when diagnosing, we should distinguish between the amount of qi and blood strength and the degree of qi stagnation and blood stasis; when treating, we should pay attention to invigorating the spleen and dispersing the stagnated liver qi, benefiting qi to activate blood circulation, and invigorating qi for ascending to restore urinary function. In addition, modern research based on TCM constitution, microscopic syndrome differentiation, and pharmacological analysis provides a scientific basis for the treatment of female stress incontinence by targeting qi and blood. [ABSTRACT FROM AUTHOR]

BACKGROUND: Studies have confirmed that icariin can promote osteogenesis and prevent and treat steroid-induced avascular necrosis of the femoral head (SANFH). Understanding the drug-target-pathway regulation mechanism will be crucial for the effective treatment of SANFH. OBJECTIVE: To review the research progress of icariin in the prevention and treatment of SANFH based on the mechanism by which icariin regulates osteogenesis through Wnt, PI3K/AKT, mTOR and estrogen signaling pathways, in order to provide ideas and reference for the effective prevention and treatment of SANFH. METHODS: Relevant literatures published from 2014 to 2020 were retrieved from PubMed, Web of Science, CNKI, VIP and WanFang databases, using the keywords of “icariin, glucocorticoid, ONFH, osteonecrosis of the femoral head, signaling pathways, pathogenesis, Wnt, Wnt/β-catenin, PI3K/AKT, mTOR, ERs.” The old and repeated literatures were excluded, and a total of 56 literatures were finally included for analysis and discussion. RESULTS AND CONCLUSION: Icariin can promote bone metabolism and improve blood circulation, and its mechanism of action in the treatment of SANFH has become the focus of research. Icariin can regulate Wnt, PI3K/AKT, mTOR, estrogen receptor and other related signaling pathways to play an osteogenic role in the treatment of SANFH. Icariin has a regulatory effect on the signaling pathways related to SANFH. However, relevant experimental studies have not yet been able to specify its mechanism of action, and there are also few relevant clinical studies. Therefore, explorations on icariin’s mechanism of action and its clinical application will be the focus and direction of research. [ABSTRACT FROM AUTHOR]

Objective·To establish a model for predicting the prognosis in patients with colorectal cancer (CRC) using single cell RNA sequencing (scRNA-seq). Methods·scRNA-seq data of patients with CRC from Gene Expression Omnibus (GEO) database was used to filter out candidate genes, which were related to metastatic CRC. The least absolute shrinkage and selection operator (LASSO) regression, Logistic regression and Kaplan-Meier analysis were used to select and evaluate the significance of the hub gene filtered out in The Cancer Genome Atlas (TCGA) database, and to develop the prognostic prediction model of CRC. Decision curve analysis and receiver operating characteristic (ROC) curve were used to assess the clinical use of the prediction model. Results·Thirty candidate genes were filtered out from the scRNA-seq data which was downloaded in GEO database, and then 9 hub genes were selected by LASSO regression in the TCGA database. The hub-gene expression was scored for each patient. The scores had significant difference between the groups with and without recurrence both in the training set and the validation set (P<0.05). In addition, Logistic regression analysis was carried out to incorporate the two independent clinical variables of primary tumor grade (T stage) and metastasis status (M stage) into the scoreclinical variable integration model. Area under curve of the ROC curve in the training set and validation set were 0.775 and 0.705, respectively. Conclusion·A relatively stable model for predicting prognosis in CRC was constructed based on the results of scRNA-seq, which has certain guiding significance for treatment decision and prognostic prediction. [ABSTRACT FROM AUTHOR]

Dongping Lake has comprehensive functions such as flood control, water transfer, tourism and irrigation. The aquatic ecosystem health status directly affects the water safety of the water receiving area. In this regard, on the basis of summarizing the research status of physical and chemical properties, eutrophication, ecological water level, community of aquatic organism and surface sediment in Dongping Lake, shortcomings were pointed out in the research. Five research contents for studying the ecological water level and the health of the aquatic ecosystem were determined for Dongping Lake, including historical data collection and systematic analysis of Dongping Lake water ecosystem, water ecosystem monitoring, ecological water level research, water ecosystem health research and water ecosystem ecological scheduling model and comprehensive repair technology research of the lake. The recent research objectives of the lake were put forward and the key scientific issues and research methods in the research process were clarified. [ABSTRACT FROM AUTHOR]

BACKGROUND: There are many studies on the pathogenesis of steroid-induced avascular necrosis of the femoral head, but the relevant mechanisms remain unclear. Local obstruction of blood flow to the femoral head is an important cause of avascular necrosis of the femoral head. OBJECTIVE: To investigate the effect of angiotensin II on avascular necrosis of the femoral head. METHODS: Male Balb/c mice were randomly divided into three groups. In the dexamethasone plus angiotensin II group, 2 mg/L dexamethasone was added in drinking water, subcutaneous implantatian with osmotic pump capsules began on the first day, followed by pumping with 0.28 mg/(kg?d) angiotensin II for 4 weeks. Dexamethasone group dosage and usage were the same as dexamethasone plus angiotensin II group. Control group had normal feed. All mice were fed for 6 weeks. RESULTS AND CONCLUSION: (1) General condition and pathological changes showed that compared with the dexamethasone and control groups, the body mass increase was significantly decreased, and the serum total cholesterol, total triglyceride, empty lacunae rate and bone volume/tissue volume were significantly increased in the dexamethasone plus angiotensin II group (P < 0.05), and the degree of osteonecrosis was more obvious. (2) Immunohistochemical results showed that the osteoprotegerin protein expression level in the dexamethasone plus angiotensin II group was significantly higher than that in the other two groups (P < 0.05), indicating that the osteoclast was more active. (3) In summary, angiotensin II can obviously promote steroid-induced avascular necrosis of the femoral head in mice. [ABSTRACT FROM AUTHOR]

Ａｎ ｉｍｐｒｏｖｅｄ ｃｕｃｋｏｏ ｓｅａｒｃｈ （ＩＣＳ） ａｌｇｏｒｉｔｈｍ ｗａｓ ｄｅｖｅｌｏｐｅｄ ｔｏ ｉｄｅｎｔｉｆｙ ｔｈｅ ｔｈｅｒｍａｌ ｄｉｆｆｕｓｉｏｎ ｃｏｅｆｆｉｃｉｅｎｔｓ ｆｏｒ ｉｎｖｅｒｓｅ ｔｒａｎｓｉｅｎｔ ｈｅａｔ ｃｏｎｄｕｃｔｉｏｎ ｐｒｏｂｌｅｍｓ ｗｉｔｈ ｈｅａｔ ｓｏｕｒｃｅｓ． Ｔｈｅ ｈｅａｔ ｃｏｎｄｕｃｔｉｏｎ ｐｒｏｂｌｅｍ ｗｉｔｈ ｈｅａｔ ｓｏｕｒｃｅ ｗａｓ ｔｒａｎｓｆｏｒｍｅｄ ｉｎｔｏ ｏｎｅ ｗｉｔｈｏｕｔ ｈｅａｔ ｓｏｕｒｃｅ． Ｔｈｅ ｄｉｒｅｃｔ ｐｒｏｂｌｅｍ ｗａｓ ｓｏｌｖｅｄ ｗｉｔｈ ｔｈｅ ｂｏｕｎｄａｒｙ ｅｌｅｍｅｎｔ ｍｅｔｈｏｄ． Ｔｈｅ ｔｈｅｒｍａｌ ｄｉｆｆｕｓｉｏｎ ｃｏｅｆｆｉｃｉｅｎｔ ｗａｓ ｔｒｅａｔｅｄ ａｓ ｔｈｅ ｏｐｔｉｍｉｚａｔｉｏｎ ｖａｒｉａｂｌｅ， ａｎｄ ｔｈｅ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｔｈｅ ｃａｌｃｕｌａｔｅｄ ｔｅｍｐｅｒａｔｕｒｅ ａｎｄ ｔｈｅ ｍｅａｓｕｒｅｄ ｔｅｍｐｅｒａｔｕｒｅ ｗａｓ ｔａｋｅｎ ａｓ ｔｈｅ ｏｂｊｅｃｔｉｖｅ ｆｕｎｃｔｉｏｎ． Ｔｈｅ ｔｈｅｒｍａｌ ｄｉｆｆｕｓｉｏｎ ｃｏｅｆｆｉｃｉｅｎｔ ｗａｓ ｏｐｔｉｍｉｚｅｄ ｔｈｒｏｕｇｈ ｍｉｎｉｍｉｚａｔｉｏｎ ｏｆ ｔｈｅ ｏｂｊｅｃｔｉｖｅ ｆｕｎｃｔｉｏｎ ｗｉｔｈ ｔｈｅ ＩＣＳ ａｌｇｏｒｉｔｈｍ． Ｃｏｍｐａｒｉｓｏｎ ｂｅｔｗｅｅｎ ｔｈｅ ｒｅｓｕｌｔｓ ｏｆ ｔｈｅ ｃｏｎｊｕｇａｔｅ ｇｒａｄｉｅｎｔ ｍｅｔｈｏｄ （ＣＧＭ）， ｔｈｅ ｃｕｃｋｏｏ ｓｅａｒｃｈ （ＣＳ） ａｌ⁃ ｇｏｒｉｔｈｍ ａｎｄ ｔｈｅ ＩＣＳ ａｌｇｏｒｉｔｈｍ ｉｎｄｉｃａｔｅｓ ｔｈａｔ ｔｈｅ ＩＣＳ ａｌｇｏｒｉｔｈｍ ｉｓ ｌｅｓｓ ｓｅｎｓｉｔｉｖｅ ｔｏ ｉｔｅｒａｔｉｖｅ ｉｎｉｔｉａｌｉｚａｔｉｏｎ ｔｈａｎ ｔｈｅ ＣＧＭ， ａｎｄ ｔｈｅ ＩＣＳ ａｌｇｏｒｉｔｈｍ ｈａｓ ｈｉｇｈｅｒ ｅｆｆｉｃｉｅｎｔ ｃｏｎｖｅｒｇｅｎｃｅ ｔｈａｎ ｔｈｅ ＣＳ ａｌｇｏｒｉｔｈｍ． Ｔｈｅ ｎｕｍｅｒｉｃａｌ ｅｘａｍｐｌｅｓ ｗｅｒｅ ｄｅｖｏｔｅｄ ｔｏ ｔｈｅ ｉｎｆｌｕｅｎｃｅｓ ｏｆ ｔｈｅ ｍｅａｓｕｒｅｄ ｐｏｉｎｔ ｎｕｍｂｅｒ， ｔｈｅ ｎｅｓｔ ｎｕｍｂｅｒ ａｎｄ ｔｈｅ ｍｅａｓｕｒｅｄ ｎｏｉｓｅ． Ｔｈｅ ｒｅｓｕｌｔ ａｃｃｕｒａｃｙ ｄｅｃｒｅａｓｅｓ ｗｉｔｈ ｔｈｅ ｍｅａｓｕｒｅｄ ｐｏｉｎｔ ｎｕｍｂｅｒ， ａｎｄ ｔｈｅ ｉｔｅｒａｔｉｏｎ ｎｕｍｂｅｒ ｄｅｃｒｅａｓｅｓ ｗｉｔｈ ｔｈｅ ｎｅｓｔ ｎｕｍｂｅｒ． Ｍｏｒｅｏｖｅｒ， ｔｈｅ ｈｉｇｈｅｒ ｔｈｅ ｍｅａｓｕｒｅｄ ｎｏｉｓｅ ｇｏｅｓ， ｔｈｅ ｌｏｗｅｒ ｔｈｅ ｒｅｓｕｌｔ ａｃｃｕｒａｃｙ ｗｉｌｌ ｂｅ． Ｔｈｅ ｒｅｓｕｌｔｓ ｓｈｏｗ ｔｈａｔ ｔｈｅ ＩＣＳ ａｌｇｏｒｉｔｈｍ ｉｓ ａｃｃｕｒａｔｅ ａｎｄ ｅｆｆｉｃｉｅｎｔ ｆｏｒ ｔｈｅ ｉｄｅｎｔｉｆｉｃａｔｉｏｎ ｏｆ ｔｈｅｒｍａｌ ｄｉｆｆｕｓｉｏｎ ｃｏｅｆｆｉｃｉｅｎｔｓ. [ABSTRACT FROM AUTHOR]