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Objective To investigate the effects of different abstinence time on the parameters of sperm DNA damage, morphology and routine semen analysis. Methods A total of 1 128 semen samples were collected from male patients in the Center for Reproductive Medicine, Zhongda Hospital, Southeast University during September 2018 and February 2021. Routine semen analysis parameters, including sperm concentration, sperm motility, percentage of progressively motile sperm (PR), etc., were detected by the CFT-9201 computer-aided sperm analysis (CASA) system. Sperm morphology was observed after Diff-Quik staining, and sperm DNA fragmentation index (DFI) was detected by sperm chromatin structure analysis (SCSA). Results Abstinence time of 1 128 patients ranged from 0 to 30 days, with a median of 3. 0 [3.0, 5.0]. In addition to sperm motility (P = 0. 117) showed normal distribution, other parameters all showed non normal distribution (P <0. 01). Abstinence time was positively correlated with semen volume (r = 0. 21, P <0. 01), sperm concentration (r = 0. 098, P < 0. 01) and sperm DFI (r = 0. 068, P < 0. 05). Although abstinence time was negatively correlated with PR, sperm motility and percentage of normal morphology sperm, there was no significant correlation (P >0. 05). Sperm DFI was negatively correlated with PR, sperm motility and percentage of normal sperm morphology (P <0. 01), but not with sperm concentration (P > 0. 05). Conclusion With the extension of abstinence time, semen volume and sperm concentration increase, and sperm DFI also increases. Therefore, appropriately shortening abstinence time can reduce the production of sperm DNA fragments. Clinicians should consider the influence of abstinence time when explaining semen analysis results to patients. [ABSTRACT FROM AUTHOR]

The use of medical terms related to reproductive testing in domestic professional journals and clinical practice is currently very no-standard and chaotic, which easily results in the misunderstanding among readers such as clinicians, patients, laboratory technicians, and science popularization worker. In order to further standardize the use of terms related to reproductive testing and promote the standardized and orderly development of reproductive testing, the Reproductive Testing Branch of China Sexology Association initiated and organized the experts in the field of reproductive testing to jointly discuss and formulate this consensus based on the connotation of terms related to reproductive testing, in combination with the interpretation and description of relevant terms published in relevant guides, manuals, consensus and literatures. The consensus involves the standardization of terms related to semen analysis, serological examination and genetic test, aiming to provide reference for medical staffs engaged in reproductive medicine, andrology and reproductive testing. [ABSTRACT FROM AUTHOR]

Sperm morphology analysis has always been the focus and difficulty in the field of reproductive testing, with some problems such as strong subjectivity, difficulty in quality control and poor mutual recognition of the results among laboratories. In order to further standardize the process of sperm morphology analysis and improve the accuracy of analysis results, the Reproductive Testing Branch of China Sexology Association initiated and organized the experts in the field of sperm morphology analysis to jointly discuss and formulate this consensus based on the 5th and 6th editions of WHO Laboratory Manual for the Examination and Processing of Human Semen, in combination with the relevant literature published in recent years and clinical practice of China. This consensus covers the contents, methods and procedures of sperm morphology analysis, criteria for normal and abnormal sperm morphology, specific sperm deformities, evaluation process of sperm morphology, and the quality control, report contents, existing problems and prospects of sperm morphology analysis, and is intended to provide reference for medical personnel engaged in reproductive medicine, andrology and reproductive testing. [ABSTRACT FROM AUTHOR]