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乳腺肿瘤及甲状腺肿瘤发病率逐年增加, 临床诊疗手段存在一定局限性。生物电阻抗技术能基于细胞层 面电特性的变化区分肿瘤组织, 具有无创/微创、精准、实时、客观、经济简便等特点, 可与临床上检查手段及治疗方 法优势互补, 提高肿瘤诊疗的精准度。生物电阻抗技术在乳腺癌诊疗中具有显著优势, 尤其在术前肿瘤诊断和术中 淋巴结转移评估方面。在甲状腺结节相关临床应用中, 生物电阻抗技术可显著提高术前和术中对甲状腺结节良恶 性的诊断准确率, 尤其对细针抽吸活检结果不明确的病例诊断具有重要的补充作用。 [ABSTRACT FROM AUTHOR]

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母系表达基因3(MEG3)是第一个被发现作为肿瘤抑制因子的长链非编码RNA(lncRNA)。近年发现MEG3与神经胶质瘤、亨廷顿病和脑卒中等神经系统疾病的发病有关,可能是神经系统疾病新的生物标志物。MEG3在神经系统疾病中的作用涉及多种机制,包括参与基因调控、促进细胞增殖、抑制细胞凋亡、促进血管生成等。MEG3在神经胶质瘤中表达缺失,主要由于DNA甲基转移酶1介导的MEG3启动子的高甲基化所致,以肿瘤抑制因子的角色发挥抗肿瘤作用。MEG3在亨廷顿病中表达下调,通过表观遗传调控多种基因的表达影响神经元活动,而在缺血性脑卒中中表达上调,可通过直接与p53基因的DBD270-281位点结合而促进p53表达,从而促进神经元凋亡。 [ABSTRACT FROM AUTHOR]

Objective To investigate the role of miR-21 and p53 in hypoxic-ischemic injury of neural cells and to explore its mechanism. Methods The cultured mouse neuroblastoma cell line N2a was randomly divided into the miR-21 (20, 40, 60 nmol/L) transfection group, negative control group, and blank control group by using Lipo2000 liposome transfection method. Ischemia-reperfusion injury model was established by oxygen glucose deprivation and reperfusion. Ap-optotic index was measured by TUNEL staining, the expression of intracellular miR-21 and p53 mRNA was detected by real-time fluorescence quantitative PCR , and the expression of p53 protein was detected by Western blotting. Results Compared with blank control group and negative control group, the relative expression of miR-21 in the transfection group was higher (P<0.05) , and the relative expression of miR-21 was concentration-dependent (P< 0.05). The apoptotic index of miR-21 (60 nmol/L) transfection group was lower than that of negative control group and blank control group (P < 0.05). The expression of p53 in the cells transfected with miR-21 (40, 60 nmol/L) was lower than that in the negative control group and the blank control group (P < 0. 05) , and the higher the concentration, the lower the expression of p53 ( P <0. 05 ). Conclusion Overexpression of miR-21 can protect neurons from hypoxic-ischemic injury, which may be related to its negative regulation of p53 expression in N2a cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the expression and clinical significance of CD68 and vascular endothelial growth factor ( VEGF) in breast cancer tissues. Methods Immunohistochemistry was used to detect the expression of CD68 and VEGF in 230 cases of breast cancer tissues and the corresponding adjacent tissues. The relationship of the expression of two proteins with the clinicopathological parameters and the recurrence of breast cancer was analyzed. Meanwhile, the correlation between the two proteins was also analyzed. Results The positive rates of CD68 and VEGF in the breast cancer tissues were significantly higher than those in the adjacent tissues ( both P <0. 01 ). The positive rate of CD68 protein in the breast cancer was closely related to the clinical stage, lymph node metastasis, and ER status of breast cancer patients ( P < 0. 05). The positive rate of VEGF protein in the breast cancer was closely related to the ER status of breast cancer patients (P <0. 05 ). The positive rates of CD68 and VEGF proteins in the recurrent patients were significantly higher than those in the non recurrent patients ( both P <0. 05 ). The expression of CD68 protein was positively correlated with the expression of VEGF (rs =0.397, P<0.01). Conclusion The expression of CD68 protein and VEGF protein increase in the breast cancer tissues, and they may be involved in the occurrence, development, and recurrence of breast cancer. [ABSTRACT FROM AUTHOR]