miR-21 在小鼠神经细胞缺氧缺血损伤中的 作用及机制.

Objective To investigate the role of miR-21 and p53 in hypoxic-ischemic injury of neural cells and to explore its mechanism. Methods The cultured mouse neuroblastoma cell line N2a was randomly divided into the miR-21 (20, 40, 60 nmol/L) transfection group, negative control group, and blank control group by using Lipo2000 liposome transfection method. Ischemia-reperfusion injury model was established by oxygen glucose deprivation and reperfusion. Ap-optotic index was measured by TUNEL staining, the expression of intracellular miR-21 and p53 mRNA was detected by real-time fluorescence quantitative PCR , and the expression of p53 protein was detected by Western blotting. Results Compared with blank control group and negative control group, the relative expression of miR-21 in the transfection group was higher (P<0.05) , and the relative expression of miR-21 was concentration-dependent (P< 0.05). The apoptotic index of miR-21 (60 nmol/L) transfection group was lower than that of negative control group and blank control group (P < 0.05). The expression of p53 in the cells transfected with miR-21 (40, 60 nmol/L) was lower than that in the negative control group and the blank control group (P < 0. 05) , and the higher the concentration, the lower the expression of p53 ( P <0. 05 ). Conclusion Overexpression of miR-21 can protect neurons from hypoxic-ischemic injury, which may be related to its negative regulation of p53 expression in N2a cells. [ABSTRACT FROM AUTHOR]