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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Geology & Exploration is the property of Xian Research Institute of China Coal Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Astrocytes play an important role in the pathology of central nervous system diseases. The phenotypic and functional changes in astrocytes suggest that it may be an effective therapeutic target for central nervous system diseases. Our previous studies have confirmed that astragaloside can inhibit the lipopolysaccharide-induced astrocyte inflammatory response. Whether astragaloside can regulate the phenotype and function of astrocytes through Notch-1 and its downstream signaling pathway remains unclear. OBJECTIVE: To explore the effect of astragaloside on astrocyte activation and inflammatory response induced by inflammation and its possible mechanism. METHODS: Cerebral cortex astrocytes derived from neonatal C57BL/6 mouse were cultured in vitro. CCK-8 assay was used to determine the optimum concentration of astragaloside and Notch active inhibitor DAPT. The astrocytes were divided into five groups: PBS group, lipopolysaccharide group, lipopolysaccharide + astragaloside group, lipopolysaccharide + DAPT group and lipopolysaccharide + DAPT + astragaloside group. The secretion level of inflammatory factors was detected by ELISA, and the level of nitric oxide was detected by Griess method. The astrocytes and splenic mononuclear cells were co-cultured in Transwell chamber to observe the migration of CD4T cells. The expression of astrocyte activation marker GFAP, A1 marker C3 and A2 marker S100A10 as well as Notch 1 and Jag-1 was detected by immunofluorescence staining. The expressions of CFB, C3, S100A10, PTX3, Notch-1, Jag-1, and Hes were detected by western blot assay. RESULTS AND CONCLUSION: (1) According to the results of CCK8 assay, the final concentration of astragaloside was selected as 25 μmol/L and the final concentration of DAPT was 50 μmol/L for follow-up experiments. (2) Compared with PBS group, interleukin-6, interleukin-12 and nitric oxide secretion levels in the lipopolysaccharide group were significantly increased (P < 0.05, P < 0.05, P < 0.01). Compared with the lipopolysaccharide group, interleukin-6 (all P < 0.05), interleukin-12 (P > 0.05, P < 0.05, P < 0.05) and nitric oxide (P < 0.05, P < 0.01, P < 0.01) secretion significantly reduced in the lipopolysaccharide + astragaloside group, lipopolysaccharide +DAPT group, lipopolysaccharide + DAPT + astragaloside group. (3) Compared with the PBS group, the expression of GFAP that is the marker of activated astrocytes and the migration of CD4 T cells were significantly increased in the lipopolysaccharide group (P < 0.01). Compared with the lipopolysaccharide group, astrocyte activation was significantly inhibited and CD4 T cell migration was significantly reduced in the lipopolysaccharide + astragaloside, lipopolysaccharide +DAPT, lipopolysaccharide + DAPT + astragaloside group (P < 0.05, P < 0.05, P < 0.01). (4) Compared with the PBS group, the expressions of A1 markers C3 and CFB in the lipopolysaccharide group were increased (P < 0.01, P < 0.05), and the expressions of A2 markers S100A10 and PTX3 were decreased (P < 0.01, P < 0.05). Compared with the lipopolysaccharide group, C3 (all P < 0.01) and CFB (both P < 0.05) were significantly reduced and S100A10 (all P < 0.01) and PTX3 (P < 0.05, P < 0.05 and P > 0.05) were increased in the lipopolysaccharide + astragaloside, lipopolysaccharide +DAPT, lipopolysaccharide + DAPT + astragaloside group. (5) Compared with the PBS group, the expressions of Jag-1, Notch-1 and Hes in the lipopolysaccharide group were significantly increased (all P < 0.01). Compared with the lipopolysaccharide group, the expressions of Jag-1 (all P < 0.01), Notch-1 (all P < 0.01) and Hes (P < 0.05, P < 0.01 and P < 0.01) were significantly reduced in the lipopolysaccharide + astragaloside, lipopolysaccharide +DAPT, lipopolysaccharide + DAPT + astragaloside group. (6) The results indicate that astragaloside can promote the transformation of astrocytes from A1 to A2 by regulating Notch-1 signaling pathway, reduce the secretion of inflammatory factors and the migration of CD4 T cells, and thus inhibit astrocyte activation and inflammatory response. [ABSTRACT FROM AUTHOR]

Copyright of China Plastics / Zhongguo Suliao is the property of Journal Office of CHINA PLASTICS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Physical Education / Tiyu Xuekan is the property of Journal of Physical Education Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guangdong Agricultural Sciences is the property of South China Agricultural University, Guangdong Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Materials Engineering / Cailiao Gongcheng is the property of Journal of Materials Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of the Beijing Institute of Fashion Technology (Natural Science Edition) is the property of Beijing Institute of Fashion Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electroplating & Finishing is the property of Electroplating & Finishing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Polymer Materials Science & Engineering is the property of Sichuan University, Polymer Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Science Research & Application is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】Aroma is one of the important quality traits of rice, which directly affects consumers’ evaluation on cooking and eating quality of rice. The selection of aromatic rice resources and the identification and utilization of aroma genes has practical significance for the genetic breeding and the development of aromatic rice. The aroma gene, Badh2, is a major gene that has been cloned to control the aroma of rice. Clarifying the distribution and utilization frequency of different alleles of Badh2 in main aromatic rice varieties planted in South China will better serve the breeding practice of aromatic rice in this area.【Method】Genotypic analysis was conducted on Badh2 in 30 rice cultivars collected from different areas in South China with the KASP functional molecular marker. The aroma of all the cultivars were tested with different evaluation methods including chewing method, KOH soaking method and HS-SPME/GC-MS. Meanwhile, the aroma ancestries of the above aromatic rice cultivars were traced, and their genetic backgrounds of aroma origins were analysed.【Result】KASP molecular markers could accurately genotype the aroma gene Badh2. The Badh2 allele variations of 30 aromatic rice varieties planted in South China were all Badh2-E7 type. The results of the aroma test showed that all Badh2-E7 type varieties possessed aroma. The aroma source parent tracing analysis of the aromatic rice varieties revealed that the aroma source parents of aromatic rice varieties were relatively simple and mainly included Xiangyaxiangzhan, Basmati 370, and etc. Xiangyaxiangzhan accounted for about 30% of the aroma source parents, and Basmati 370 accounted for about 13% of the aroma source parents【. Conclusion】 Large scale and rapid identification of aromatic rice can be achieved through KASP genotyping technology. The Badh2 alleles of 30 aromatic rice varieties collected in South China are all the genotype of Badh2-E7, and the simplicity of the genetic background of the aroma gene may result from the single source of the aroma parent. In future aromatic rice breeding, new aroma genes can be introduced to enrich the genetic background of rice aroma. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the diagnosis of variable working condition gear faults, traditional deep learning models have low diagnostic accuracy and poor generality due to the problems of diverse gear operating conditions, few fault sample data, significant differences in data distribution, and imbalanced fault data. To address these problems, a variable working condition gear fault diagnosis (VWFD) method (modal) based on meta-learning techniques was proposed. Firstly, the original vibration signals of the gear were resampled using overlapping sampling to increase the number of fault samples. Secondly, the resampled fault data was transformed into time-frequency feature maps through short-time Fourier transform (STFT), making the data form more suitable for the model’s input to extract more complete fault features. Then, the Inception modules were introduced into the prototype network based on meta-learning technology to improve its feature expression ability and obtain more comprehensive fault feature information. Finally, various fault metric prototypes were established through the optimized prototype network, and fault classification was performed using a metric classifier to achieve gear fault diagnosis under variable working conditions. A series of comparative experiments were designed to verify the rationality of the proposed model structure and the introduction position and quantity of Inception modules, and the experimental results were analyzed. The research results show that the VWFD method can achieve higher diagnostic accuracy than other fault diagnosis methods. For example, in the 5-way 5-shot experiment group with the same load and different speed types of variables working conditions, the average diagnostic accuracy of VWFD is as high as 91.26%, while the diagnostic accuracy of support vector machine (SVM), convolutional neural networks (CNN), and prototypical nets (PN) are only 74.48%, 87.22%, and 89.56%, respectively. [ABSTRACT FROM AUTHOR]

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Materials Engineering / Cailiao Gongcheng is the property of Journal of Materials Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

AIM: To investigate the effect of astragaloside IV （AST IV） on cognition and pathology in Alzheimer disease （AD） mouse model, and to explore its underlying possible mechanism. METHODS: The APP/PS1 transgenic mice were randomly classified into AD+AST IV and AD groups, and C57BL/6 wild-type （WT） mice were used as control group（ WT group）. The mice were treated by intragastric administration for 2 months. Morris water maze and Ymaze tests were performed to evaluate the spatial cognitive function of the mice. Nissl staining was used to detect the number and morphology of neurons, while immunofluorescence staining was used to detect neuronal nuclear antigen （NeuN） and amyloid β-protein （Aβ） levels. The expression levels of neurite outgrowth inhibitor A （NogoA）, Nogo-66 receptor （NgR）, p75 neurotrophin receptor （p75NTR）, leucine rich repeat and immunoglobin-like domain-containing protein-1 （LINGO-1）, cyclic adenosine monophosphate （cAMP）, and protein kinase A （PKA） in the whole brain were detected by Western blot. RESULTS: Treatment with AST IV significantly improved cognitive function in APP/PS1 mice, and enhanced learning, memory and exploration abilities. Compared with WT mice, APP/PS1 mice exhibited increased Aβ deposition in the cerebral cortex and hippocampus （P<0. 01）, significant loss of Nissl bodies （P<0. 05 or P<0. 01）, and decreased number of neurons （P<0. 05）. However, AST IV treatment significantly reduced Aβ deposition （P<0. 05） and the loss of Nissl bodies（ P<0. 05）, and increased the number of neurons（ P<0. 05）. Compared with WT mice, the expression levels of NogoA, NgR, p75NTR and LINGO-1 in the whole brain tissue of APP/PS1 mice were significantly increased （P<0. 05 or P<0. 01）, whereas those of cAMP and PKA were significantly decreased （P<0. 05 or P<0. 01）. However, AST IV treatment significantly inhibited the expression of NogoA, NgR, p75NTR and LINGO-1 （P<0. 05 or P<0. 01）, and increased the expression of cAMP and PKA（ P<0. 05）. CONCLUSION: Treatment with AST IV reduced Aβ deposition and neuronal damage in the brains of APP/PS1 mice by inhibiting the expression of NogoA and NgR/p75NTR/LINGO-1 receptor complex and up-regulating the cAMP/PKA signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Railway Standard Design is the property of Railway Standard Design Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Polymer Materials Science & Engineering is the property of Sichuan University, Polymer Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Feed Research is the property of Editorial Board of Feed Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Abnormal mitochondrial dynamics is closely related to oxidative stress after affecting neurodegenerative diseases. Our previous studies have shown that eriodictyol can reduce nerve injury in Alzheimer’s disease, but whether it has a regulatory effect on mitochondrial dynamics is not clear. OBJECTIVE: To explore the mechanism of eriodictyol on SH-SY5Y cell apoptosis induced by hydrogen peroxide (H2O2). METHODS: SH-SY5Y cells were divided into three groups: PBS control group, H2O2 (250 μmol/L) group, and H2O2 (250 μmol/L) plus eriodictyol (10 μmol/L) treatment group (eriodictyol group). The intervention was performed for 24 hours. The kits were utilized to observe the level of malondialdehyde and the activity of superoxide dismutase. Cell morphology was observed under the microscope. TUNEL staining was used to observe the apoptosis. JC-1 staining was used to observe mitochondrial membrane potential. The expression levels of apoptotic proteins and mitochondrial fusion and fission protein-related proteins were detected by immunofluorescence staining and western blot assay. RESULTS AND CONCLUSION: (1) A significant decrease of malondialdehyde levels and a significant increase of superoxide dismutase activity were found in eriodictyol treated group, when compared with the H2O2 group. (2) Compared with the PBS control group, the H2O2 group showed decreased cell density, cytosolic hypertrophy, increased apoptosis, decreased mitochondrial membrane potential, decreased Bcl-2 expression, and increased Bax expression. Compared with the H2O2 group, above indexes were significantly improved in the eriodictyol group. (3) Compared with PBS control group, the expression levels of mitochondrial fission-related proteins dynamin-related protein 1 (p-DRP1) and mitochondrial fission protein 1 (FIS1) were elevated and the expression levels of mitochondrial fusion-related proteins optic atrophic protein 1 (OPA1) and mitofusin 2 (Mfn2) were decreased in the H2O2 group. Compared with the H2O2 group, eriodictyol treatment decreased the expression levels of p-DRP1 and FIS1 and increased the expression of OPA1 and Mfn2. (4) The results indicate that eriodictyol can inhibit H2O2-induced apoptosis in SH-SY5Y cells by regulating mitochondrial dynamics. [ABSTRACT FROM AUTHOR]

Acute pancreatitis often progresses rapidly, and if it is not controlled in time, it may lead to multiple organ injury and even death due to organ failure. At present, there is still a high proportion of patients with acute pancreatitis who died due to liver failure. The liver and the pancreas are interrelated physiologically and affect each other pathologically. This article elaborates on the molecular mechanism of liver injury in acute pancreatitis from the six aspects of the physiological relationship between the pancreas and the liver, cytokines, inflammatory response, oxidative stress, microcirculation disturbance, and intestinal flora translocation. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cell therapy can effectively fill the neurons lost in Alzheimer’s disease patients, but the specific mechanism of action needs to be further clarified. OBJECTIVE: To explore the therapeutic effects of bone marrow mesenchymal stem cells in APP/PS1 double transgenic mice with Alzheimer’s disease and its mechanism. METHODS: APP/PS1 double transgenic mice were selected as the research objects and were randomly divided into model and bone marrow mesenchymal stem cell groups (n=10 per group). Ten wild-type C57BL/6 mice of the same month and the same sex were selected as the normal control group. The mice were treated with bone marrow mesenchymal stem cells (1×106 /time) or normal saline, and repeated administration once a week, for 2 months via nasal cavity. Morris water maze test was applied to examine cognitive function of mice. Western blot assay was used to detect the expression of related factors of pathological markers, inflammatory molecules and oxidative stress proteins in the brain tissues of mice. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells effectively improved the cognitive dysfunction of Alzheimer’s disease mice, increased the expression of APP protein and decreased expression of phosphorylated tau protein, reduced the expression of Toll like receptor 2, inducible nitric oxide synthase, nuclear transcription factor kappa B and increased the expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. It is concluded that bone marrow mesenchymal stem cells have a potential role in the treatment of Alzheimer’s disease; the mechanism exerts the effects of the anti-inflammation and antioxidation through nuclear factor erythroid 2-related factor 2/heme oxygenase-1 signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Materials Engineering / Cailiao Gongcheng is the property of Journal of Materials Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A triblock copolymer with mussel-inspired adhesive ability, poly(acryl hydrazide)-b-poly(N-(3, 4- dihydroxyphenylethyl) acrylamide)-b-poly(monomethoxypolyethylene glycol acrylate) (PAH-b-PAD-b-PmPEGA, abbreviated as HDP), was designed and synthesized via reversible addition fragmentation chain transfer polymerization process from three monomers, including 1-tert-butcarbonyl-2-acrylhydrazide(Boc-AH), N-(3, 4-dihydroxyphenylethyl) acrylamide(DA) and poly(ethylene glycol) methyl ether methacrylate (mPEGA). The copolymer was used for decorating gold nanorod (GNR) to obtain GNR-based nanocarrier. Chemotherapeutic drug doxorubicin (DOX) was conjugated onto the nanocarrier by an acid-labile hydrazone linkage, resulting in HDP-GNR-DOX nanodrug. The physicochemical properties of the nanocarrier, such as structure, stability, photothermal performance, and pH-responsive drug release, were characterized. Moreover, the in vitro anti-tumor effect of the nanodrug towards breast cancer cells (MCF-7) was evaluated. Results showed that the DOX content of the nanodrug was as high as 8.1% and the nanodrug exhibited excellent photothermal conversion ability, favorable stability and pH-responsive drug release behavior. Importantly, the results of cellular experiments demonstrated that the nanodrug could be effectively internalized by MCF-7. In the case of near infrared irradiation, the nanodrug showed high apoptosis-inducing ability on MCF-7, achieving highly efficient photothermal-chemotherapy of breast cancer. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Functional Polymers is the property of Journal of Functional Polymers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: More and more evidences show that ROCK signaling pathway is involved in inflammation and oxidative stress. Fasudil is an effective Rho kinase inhibitor. Our previous series of studies have proved that fasudil has neuroprotective effects. However, it is still unclear that whether fasudil has protective effect on oxidative damage to astrocytes and its possible mechanisms. OBJECTIVE: To explore the protective effect and mechanism of fasudil on lipopolysaccharide-induced oxidative injury in astrocytes. METHODS: Primary C57BL/6 mouse astrocytes were cultured and divided into PBS control group, lipopolysaccharide stimulation group (1 mg/L), lipopolysaccharide (1 mg/L) combined with fasudil (15 mg/L) treatment group. After 24 hours of treatment, MTT assay was used to detect the viability of astrocytes. The content of malondialdehyde and the activity of superoxide dismutase in astrocytes were measured using the kit to evaluate the level of oxidative stress. Immunofluorescence staining was used to detect the expression of cleaved-caspase-3, the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of heme oxygenase-1 (HO-1) in astrocytes. Western blot assay was used to detect the total Nrf2, nuclear Nrf2 and HO-1 protein levels. RESULTS AND CONCLUSION: (1) Compared with the PBS control group, the astrocyte viability in the lipopolysaccharide stimulation group was reduced, and fasudil treatment improved the cell damage induced by lipopolysaccharide. (2) Compared with the PBS control group, lipopolysaccharide resulted in a significant decrease in the activity of superoxide dismutase and a significant increase in the level of malondialdehyde in astrocytes (P < 0.01). Fasudil significantly increased the activity of superoxide dismutase (P < 0.01) and reduced the level of malondialdehyde (P < 0.01). (3) Compared with the PBS control group, the expression of cleaved-caspase-3 was significantly increased in the lipopolysaccharide stimulation group. Compared with the lipopolysaccharide stimulation group, the expression of cleaved-caspase-3 was decreased in the lipopolysaccharide combined with fasudil treatment group. (4) Fasudil could promote the nuclear translocation of Nrf2 and significantly increase the expression of total Nrf2, nucleus Nrf2 and HO-1 after lipopolysaccharide stimulation. (5) The results indicate that fasudil can inhibit lipopolysaccharide-induced astrocytic injury by regulating the Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]